A Model of Systemic Inflammation to Study Neuroinflammation.
Increasing evidence suggests that neurodegeneration occurs in part because the environment is affected during disease in a cascade of processes collectively termed neuroinflammation. This is a reactive response of the central nervous system against noxious elements that interfere with tissue homeostasis. Neuroinflammation is mediated by inflammatory molecules released by microglial cells. Understanding and controlling interactions between the immune system and microglial activation might represent the key to prevent or delay the onset of central nervous system diseases. This chapter details techniques to generate and characterize an in vivo model of neuroinflammation based on a single intraperitoneal injection of lipopolysaccharide, which can be used to understand the wide variety of cellular and molecular mechanisms of neuroinflammation, as well as to identify new therapies by testing the anti-inflammatory properties of synthetic and natural molecules.