Immunodiffusion: detection of a murine leukemia virus (Rauscher).
Homologous and heterologous antiserums from several species of animals have been prepared against the Rauscher murine leukemia virus. The Ouchterlony technique, adapted to very small quantities, has been used to demonstrate at least two or three antigens in Rauscher virus preparations. Both infected-host materials and tissue-culture fluids were used as antigens. When monkey antiserum was used, one of the Rauscher virus antigens cross-reacted with an antigen in the virus strains isolated by Friend and Moloney, but there was apparently no reaction with the Moloney virus when guinea-pig antiserum was used.