Embryos with "heteroplasmy," or the presence of both maternal and paternal mitochondrial DNA generated!

A new study published in the Proceedings of the National Academy of Sciences (PNAS) has succeeded in creating embryos with "heteroplasmy," or the presence of both maternal and paternal mitochondrial DNA. This new innovation will allow scientists to study treatments for mitochondrial diseases in humans as well as the significance of mitochondrial inheritance for livestock.

When parents pass along their genes to their children, most of the DNA from the mother and father is evenly divided. However, children only receive one type of DNA, called mitochondrial DNA, from their mothers, while the fathers' mitochondrial DNA is naturally removed from the embryos.

"As many as 4,000 children are born in the U.S. every year with some form of mitochondrial disease, which can include poor growth, loss of muscle coordination, learning disabilities and heart disease," senior author said. "Some scientists believe some of these diseases may be caused by heteroplasmy, or cells possessing both maternal and paternal mitochondrial DNA. We have succeeded in creating this condition of heteroplasmy within pig embryos, which will allow scientists to further study whether paternal heteroplasmy could cause mitochondrial diseases in humans."

For their study, researchers identified two separate ubiquitin-binding proteins, called SQSTM1 and valosin-containing proteins (VCP), within embryos they believed were responsible for removing paternal, sperm-contributed mitochondria and their genetic cargo.

They experimented by inhibiting SQSTM1 and VCP separately, but found that even when one protein was incapacitated, the other protein still carried out the duty of disposing of the paternal mitochondria inside the fertilized egg. However, when they inhibited both proteins simultaneously, the paternal mitochondria were not removed and remained within the embryos.

"This research is important because we now know for sure what processes lead to the deletion of paternal mitochondrial DNA from embryos," senior author said. "This knowledge will enable us to further explore how some children may develop devastating mitochondrial diseases. From there, we can create treatments and therapies that may help prevent or reduce the effects of heteroplasmy and other mitochondrial disorders."

Rating

Unrated

Rating:

Comments

Main Comment

By Brahma,
posted August 24th 2016, 3:14 PM

Rating:

This is an important step forward towards controlling mitochondrial borne human diseases or disorders in human subjects. This technique in all probability would supplement the existing mitochondrial replacement therapy.

Don't fill this field, it is here to catch spam-bots that fill in forms automatically