1. Introduction {#sec1}
===============

Infection of small ruminants with gastrointestinal nematodes (GINs) remains a serious pathological problem across the world affecting animal health, welfare and production, as there is now a critically high level of drug resistance ([@bib16]). These GINs are able to develop resistance to new synthetic (anthelmintic, AH) drugs within a few years ([@bib33]) and, therefore, sustainable livestock farming can no longer rely on deworming with AH drugs. New approaches for the sustainable control of GIN seek to lower parasite numbers to a manageable level or to modify their biological development and life cycle, rather than to eliminate them completely. Medicinal plants have been used since antiquity to treat worm infections ([@bib15]) and anti-parasitic properties of plants against GIN have been linked to the presence of proteinases ([@bib28]) and other secondary plant metabolites. Plant phenolics, flavonoids and condensed tannins have received considerable attention over the last few years ([@bib5; @bib13; @bib14]) as plants that produce these compounds grow in all regions of the world. Condensed tannins (CT; *syn*. proanthocyanidins) can have direct and indirect AH effects ([@bib22; @bib14]), as well as having the potential to enhance the host\'s innate immune response ([@bib32]). The hypothesis for direct effects of polyphenolic compounds has been substantiated by several *in vitro* assays against different life-cycle stages ([@bib4; @bib26]).

Condensed tannins are oligomers or polymers of flavan-3-ols and are classified into different subgroups. The two major CT types are procyanidins (PC), which have two OH-groups, and prodelphinidins (PD), which have three OH-groups in the B-ring ([Fig. 1](#fig1){ref-type="fig"}). The stereochemistry at the heterocyclic C-ring gives rise to either 2--3 *cis-* or 2--3 *trans-*flavan-3-ols and the average polymer length of CT mixtures is described in terms of mean degree of polymerisation (mDP). It is important to note that most condensed tannin-containing forages tend to contain complex mixtures of PC and PD homo- and hetero-polymers that are difficult to separate. However, a few plants specialise in the synthesis of either PCs or PDs and can thus serve as valuable sources for the different tannin types and as research tools for probing structure--activity relationships.

Since tannins and other flavonoids co-occur in plants, it is pertinent to investigate their combined potencies. To this end, we selected polyphenols, which are known to occur in willow (*Salix* spp, Salicaceae), as this species represents a potentially valuable nutraceutical forage ([@bib24; @bib9; @bib25]). Moreover, breeding programmes are currently focussing on increasing the *Salix* wood production as a renewable source of energy ([@bib18]) and, therefore, it is timely to explore, which phenolic compositions can contribute most to the AH properties of the leaf by-products. The phenolic composition of willow leaves has been studied already by several groups ([@bib17; @bib27; @bib10]) and we recently reported that willow tannins cover a wide range of PC/PD ratios (from 15/85 to 98/2) and *cis*/*trans* flavan-3-ol ratios (from 2/98 to 81/19) ([@bib11]). We now describe the isolation of distinct PC and PD types from specialist CT plant sources in order to cover the full spectrum of *Salix* tannins and tested their AH effects in the presence of commercially available phenolic monomers that are known to be present in *Salix* leaves. This allowed us to test two hypotheses; the first stated that CT and other naturally occurring polyphenolic monomers exert synergistic inhibitory effects on the *in vitro* L3 exsheathment of *Haemonchus contortus* and the second that these AH interactions depend on CT types.

2. Materials and methods {#sec2}
========================

2.1. Chemicals and plant materials {#sec2.1}
----------------------------------

Arbutin, luteolin and taxifolin were purchased from Apin Chemicals Ltd (Abingdon, U.K.); vanillic acid and naringenin from Alfa Aesar (Blackpool, U.K.); gallocatechin and quercetin from Sigma--Aldrich (Gillingham, U.K.); phosphate buffered saline (PBS) from bioMerieux (Lyon, France), Milton solution, sodium hypochlorite 2% w/v and sodium chloride 16.5% w/v from Milton (Inibsa laboratorios, Barcelona, Spain). Goat willow (*Salix caprea*), black currant (*Ribes nigrum*) and red currant (*Ribes rubrum*) leaves were collected in July and August 2012. Tilia (Linden) flowers (*Tilia* × *europaea*) were purchased from Flos (Mokrsko, Poland). These plant samples were chosen as they allowed the testing of different tannin types, all of which are known to occur in willow.

2.2. Preparation of plant extracts and tannin fractions {#sec2.2}
-------------------------------------------------------

Freeze-dried, powdered plant material (25 g of each species) was extracted with an acetone:water mixture (7:3, v/v; 300 ml) ([@bib35]) and filtered. The filtrate was extracted with dichloromethane (250 ml) and the organic phase discarded. The aqueous phase was rotary evaporated under vacuum at 40 °C to remove residual organic solvents and then lyophilised. The freeze-dried extract was re-dissolved in distilled water, filtered under vacuum to remove insoluble particles and applied to a Sephadex LH-20 column. Fraction 1 was eluted with acetone/water (3:7, v/v) and fraction 2 with acetone/water (1:1, v/v). Acetone was removed in a rotary evaporator and the aqueous residue freeze-dried. Tannins were quantified and characterized by thiolysis with benzylmercaptan ([@bib12]). Tannin composition was determined in terms of the percentage of PCs and PDs, *cis*- and *trans-*flavan-3-ol subunits and mean degree of polymerisation (mDP), which were calculated according to [@bib12].

2.3. Bioassays {#sec2.3}
--------------

### 2.3.1. Haemonchus contortus strains {#sec2.3.1}

Third-stage larvae (L3) were obtained from sheep or goats infected with two monospecific strains of *H. contortus* that were susceptible to AH drugs ([@bib1]). The Juan strain was obtained from donor sheep, which were kept indoors and infected monospecifically with *H. contortus*, and the larvae had been maintained in the laboratory for four months. This strain was used initially to investigate the effects of four F1- and four F2-tannin fractions and of several phenolic compounds. The INRA strain was obtained from monospecifically infected goats and the larvae had been maintained in the laboratory for 1 month before use. This strain was used to repeat experiments with the F2 tannin fractions, two of the flavonoids (quercetin and luteolin) and also with the tannin-flavonoid mixtures. The facilities hosting the animals and the trial was performed according to French ethical and welfare rules (agreement number C 31 555 27 of 19 August 2010).

### 2.3.2. Larval exsheathment inhibition assay (LEIA) {#sec2.3.2}

The LEIA was performed as described previously ([@bib4]). Briefly, third-stage larvae were incubated with the tannin or flavonoid test solutions at 23 °C for 3 h, washed with PBS and centrifuged 3 times. The pelleted larvae were suspended in 200 μl water (ca 360--420 nematode L3 larvae) and 40 μl were removed to count the proportion of exsheathed larvae at 0 min. The remaining larvae (160 μl) were then subjected to the artificial exsheathment process by adding 40 μl of a Milton solution (2% w/v sodium hypochlorite and 16.5% sodium chloride) diluted in PBS, where the Milton:PBS ratio varied from 1 in 250 to 1 in 330 (this ratio was tested at the start of each experiment per batch in order to ensure 100% exsheathment within 60 min in the negative control). The numbers of ensheathed and exsheathed larvae were counted under a microscope at 0, 20, 40 and 60 min after contact with the Milton solution to investigate the proportion of exsheathed larvae over time. The tannin fractions were prepared as final serial concentrations of 600, 300, 150, 75, 37.5 μg fractions/ml and all other phenolic compounds at 1000, 500, 250, 100, 60 μM in PBS (0.1 M phosphate, 0.05 M NaCl, pH 7.2). The experiments with tannin-flavonoid mixtures were conducted using mixed solutions containing the same final concentrations of F2 fractions (600, 300, 150, 75, 37.5 μg fractions/ml) and combined with a fixed flavonoid concentration (either 60 or 30 μM quercetin or luteolin) in PBS. Four replicates were performed for each test solution and the negative control, L3 in PBS, was run in parallel.

2.4. Statistical analysis {#sec2.4}
-------------------------

The 50% effective concentration (EC~50~-value) was calculated using PoloPlus^©^ (version 1.0; LeOra Software Company, Petaluma, California, USA). These EC~50~-values are reported on the basis of tannin content in the F1 and F2 fractions (see [Table 1](#tbl1){ref-type="table"}). To assess possible synergistic effects of the tannin/flavonoid combinations, we used the observed inhibitory effects of the individual treatments to calculate the additive inhibitory effect that would be predicted to be achieved by the mixture under the assumption of independent action, using the definition of Bliss additivity ([@bib6]). The observed effect of the mixture was then compared to this predicted additive effect, with deviations from the predicted values indicating either synergy (greater effect) or antagonism (lower effect) ([@bib34]).

3. Results {#sec3}
==========

3.1. Condensed tannin analysis {#sec3.1}
------------------------------

The tannin fractions from the four plant species differed significantly in composition as shown in [Table 1](#tbl1){ref-type="table"}. The CT contents ranged from 43.4 to 99.8 g CT/100 g fraction; the mean degree of polymerisation (mDP) from 2.3 to 19.0; the tannin PC/PD ratios from 5.1/94.9 to 100/0 and the *cis/trans* flavan-3-ol ratios from 6.0/94.0 to 95.4/4.6. The fractions that eluted early from the Sephadex LH-20 column (F1 fractions) had lower CT contents (43.4--70.4 g CT/100 g fraction) and mDP-values (2.3--5.4) than the later eluting F2 fractions (CT-contents were 88.9--99.8 g CT/100 g fraction and mDP values were 6.4--19.0). Goat willow leaf and tilia flower tannins were mostly PCs, but black and red currant leaf tannins were mostly PDs. Stereochemistry added an additional distinguishing feature: red currant and tilia tannins contained predominantly *cis*-flavan-3-ols, but black currant and goat willow tannins had mostly *trans*-flavan-3-ols.

3.2. In vitro larval exsheathment inhibition by condensed tannins and other phenolic compounds {#sec3.2}
----------------------------------------------------------------------------------------------

All tannin samples were first screened with four-month old larvae (Juan *H. contortus* strain from sheep) in the *in vitro* larval exsheathment inhibition assay. This experiment was then repeated with one-month old larvae (INRA *H. contortus* strain maintained on goats) and focussed only on the more potent F2 fractions. Results with the Juan strain gave 1.7--4.6-fold lower EC~50~-values than with the INRA strain ([Table 2](#tbl2){ref-type="table"}). Further experiments will be necessary to elucidate the underlying causes of this difference, such as larval age or origin, as older larvae from sheep were more sensitive to CTs (P \< 0.05) than younger larvae from goats.

These initial studies with the Juan strain showed that the EC~50~-values were generally lower with the F2 tannins than the corresponding F1 fractions from each plant source ([Table 2](#tbl2){ref-type="table"}). The Table and [Fig. 2](#fig2){ref-type="fig"} also show that the PDs from black and red (in the web version) currant leaves tended to be more active than the PCs from tilia flowers and goat willow leaves.

The phenolic monomers differed greatly in their ability to inhibit larval exsheathment: arbutin, vanillic acid and taxifolin had no activity, whereas gallocatechin showed some activity at 1000 μM. However, naringenin, quercetin and luteolin were highly effective at 250 μM. Quercetin and luteolin were chosen (at 30 and 60 μM) for further investigation in combination with the different CTs.

3.3. In vitro larval exsheathment inhibition by condensed tannin-quercetin mixtures {#sec3.3}
-----------------------------------------------------------------------------------

Preliminary experiments using mixtures of serial CT concentrations with 200 or 100 μM quercetin completely inhibited exsheathment. Therefore, much lower quercetin concentrations (60 and 30 μM) were tested in the mixture experiments. The EC~50~-values of the mixture experiments with CTs and quercetin (30 and 60 μM) are shown in [Table 2](#tbl2){ref-type="table"}. The mixtures of PC fractions with the high quercetin concentration (60 μM) gave 3.8--5.7 fold lower EC~50~-values than these tannin fractions on their own; at the lower quercetin concentration (30 μM) the mixture gave a 2.3 fold lower EC~50~-value than the tilia tannin fraction. However, the mixtures of PD fractions with either quercetin concentration gave only 1.6--2.0 fold lower EC~50~-values than tannin fractions on their own.

The inhibition profiles generated by the mixtures revealed a dose--response relationship between *H. contortus* exsheathment and CT or quercetin concentrations as illustrated for the red currant PDs and quercetin ([Supplementary Fig. 1](#appsec1){ref-type="sec"}). Mixed solutions containing low CT concentrations (37.5 and 75 μg of fractions/ml) combined with either quercetin concentration (30 or 60 μM) gave inhibition profiles that were similar to the quercetin solutions on their own (e.g. [Supplementary Fig. 1A and B](#appsec1){ref-type="sec"}). However, mixed solutions with higher CT concentrations (300 and 600 μg of fractions/ml) and either quercetin concentration achieved \> 90% inhibition. Quercetin with 150 μg of CT fractions/ml gave intermediate profiles (e.g. [Supplementary Fig. 1C](#appsec1){ref-type="sec"}). [Fig. 2](#fig2){ref-type="fig"} depicts the separate effects of each of the PC and PD fractions (at 300 μg of fraction/ml) on exsheathment and two quercetin or luteolin concentrations (30 and 60 μM). It also shows the combined effects of the mixed CT + flavonoid solutions. Tilia and goat willow PCs gave very similar inhibition profiles ([Fig. 2](#fig2){ref-type="fig"}A and B) whether on their own or mixed with quercetin. Red currant PDs, however, were more potent on their own than the black currant PDs ([Fig. 2](#fig2){ref-type="fig"}C and D). Tilia PCs were less active than black currant PDs on their own, but in the presence of flavonoids, all tilia PCs proved more active than the PD + flavonoid mixtures; this difference was significant (*P* \< 0.05) for the luteolin combinations at 30 μM (see next section).

3.4. In vitro larval exsheathment inhibition by condensed tannin-luteolin mixtures {#sec3.4}
----------------------------------------------------------------------------------

Experiments were also performed with CT + luteolin mixtures utilising the PC tannins from tilia flowers and PD tannins from black currant leaves. The EC~50~-values of these experiments are shown in [Table 2](#tbl2){ref-type="table"}. The mixture of tilia PC tannins with the higher luteolin concentration (60 μM) completely inhibited exsheathment so that the EC~50~-value could not be calculated. However, with the lower luteolin concentration (30 μM), the mixture gave a 4.7 fold lower EC~50~-value than the PC fraction on its own (75.9 vs 356 μg/ml). In comparison, the mixtures of black currant PDs with high (60 μM) and low (30 μM) luteolin concentrations were 8.9 and 1.6 fold more active than the PDs on their own (EC~50~-values of 31.2 and 170 vs 277 μg/ml). [Fig. 2](#fig2){ref-type="fig"}E depicts the separate effects of the tilia PCs and luteolin, and also the combined effects of the CT + luteolin mixtures. Luteolin at 30 μM produced a similar inhibition as the PCs on their own (300 μg fraction/ml), but luteolin at 60 μM was much more active.

Mixed solutions of lower CT concentrations (37.5, 75 and 150 μg fraction/ml) with the low luteolin (30 μM) concentration gave inhibition profiles similar to luteolin alone (data not shown), whereas mixed solutions at higher CT concentrations (300 and 600 μg fraction/ml) achieved \> 95% inhibition (see [Fig. 2](#fig2){ref-type="fig"}E and F for the profiles at 300 μg fraction/ml). In contrast, combinations of all CT concentrations with luteolin at 60 μM gave generally greater than 95% inhibition ([Fig. 2](#fig2){ref-type="fig"}E and F give two examples). The tilia PC + luteolin and the black currant PD + luteolin mixtures (at 30 and 60 μM) gave similar inhibition patterns ([Fig. 2](#fig2){ref-type="fig"}E and F); however, there is some evidence that the mixtures with 60 μM luteolin and low PD concentrations (37.5, 75 and 150 μg fraction/ml) were not as effective as the corresponding PC mixtures (see also [Fig. 4](#fig4){ref-type="fig"}A and B).

3.5. Synergistic effects between condensed tannins and quercetin {#sec3.5}
----------------------------------------------------------------

The CT-quercetin mixtures revealed synergistic effects with CTs. [Fig. 3](#fig3){ref-type="fig"} shows that the observed inhibition was much greater than the predicted additive inhibition calculated from the separate CT and quercetin data points (i.e. predicted additive effect): synergy was demonstrated between quercetin and tilia PCs ([Fig. 3](#fig3){ref-type="fig"}A) at 150 and 300 μg fraction/ml after 60 min, and with goat willow PCs ([Fig. 3](#fig3){ref-type="fig"}C) at 300 μg fraction/ml. Synergy was also observed with black currant PDs at 300 μg fraction/ml ([Fig. 3](#fig3){ref-type="fig"}B) and red currant PDs at 150 and 300 μg fraction/ml ([Fig. 3](#fig3){ref-type="fig"}D). In addition, synergy could be detected at 40 min but at a slightly lower level (not shown).

3.6. Synergistic effects between condensed tannins and luteolin {#sec3.6}
---------------------------------------------------------------

Two of the slightly weaker AH tannins, tilia PCs and black currant PDs, were also evaluated for synergy at two luteolin concentrations. [Fig. 4](#fig4){ref-type="fig"} shows the effects after 60 min. In the presence of 60 μM luteolin, tilia PCs exhibited clear synergy at all concentrations, but black currant PDs showed synergy only at 300 μg fraction/ml. At the lower luteolin concentration (30 μM), tilia PCs acted synergistically at 300 μg fraction/ml and black currant PDs at 150 and 300 μg fraction/ml.

4. Discussion {#sec4}
=============

We chose the LEIA as it has advantages over other bioassays in that it is sensitive, reproducible and relevant to processes that occur *in vivo* ([@bib2; @bib3]). This assay was, therefore, chosen for evaluating the different CTs and flavonoids. The main aim was to investigate their combined effects as tannin-containing plants usually contain mixtures of CTs and other flavonoids.

PCs and PDs are the most common condensed tannins in forages and browse plants ([@bib21; @bib31]) and tend to occur as complex combinations of homo- and hetero-polymers ([@bib29]) that are difficult to separate. However, well-defined tannin types are needed for unravelling the relationships between tannin structures and AH activities. A more straightforward approach, therefore, is to exploit plants that specialise in producing relatively pure but different tannin types ([@bib35]). The ultimate aim of this strategy is to provide plant breeders with targets for enhancing the nutraceutical properties of new animal feeds or varieties. Thus we isolated tannins as relatively pure homopolymers of 95.7--100% PCs and 89.9--94.7% PDs ([Table 1](#tbl1){ref-type="table"}) and also obtained two procyanidin subsets and two prodelphinidin subsets with low and high *cis/trans* flavan-3-ol ratios. Quercetin and luteolin are widely distributed in plants ([@bib38; @bib20]) and represent two important flavonoid subgroups, i.e. flavonols and flavones, respectively ([Fig. 1](#fig1){ref-type="fig"}), and were thus chosen for the combination experiments with CTs. Initial studies tested the AH effects of the four tannin types at several concentrations. Whilst the results confirmed that PDs tended to be better anthelmintics than PCs ([@bib23; @bib7; @bib26]), little is known about whether stereochemistry of the flavan-3-ol subunits has any effect. Our data provided an indication that the *cis/trans* stereochemistry of the flavan-3-ol subunits did not impact on AH activities ([Table 1](#tbl1){ref-type="table"}) in agreement with a previous study ([@bib35]), although further work will be needed as the average polymer sizes also varied for the PD subset (mDP-values were 10 and 19).

The data showed that tannin concentrations, which ranged from 43.4 to 99.8 g CT/100 g fraction, were not correlated with EC~50~ values. This points to the possibility that 'impurities' in these fractions may have moderated tannin activities. Typical impurities in aqueous acetone extracts and partially purified tannin fractions tend to consist of low molecular weight phenolics and flavonoids ([@bib31]).

Given that we observed increased inhibition of exsheathment with a mixture of tannins and quercetin or luteolin, we next quantified whether this effect was additive or synergistic in nature. Synergy refers to phenomena where two or more agents together produce an effect greater than would be predicted from their individual contributions. There are various mathematical models available to assess synergy, all of which have advantages and disadvantages ([@bib37]). Using the Bliss model ([@bib34]), we observed synergistic effects between all CTs and quercetin or luteolin in terms of AH activity during the exsheathment of 3rd stage *H. contortus* larvae ([Figs. 3 and 4](#fig3 fig4){ref-type="fig"}). These CTs were tested at serial concentrations of 37.5, 75, 150, 300, 600 μg fraction/ml, which are physiologically relevant as extractable tannins range from 350 to 900 μg/ml in the sheep gut ([@bib30; @bib5]). However, no such information was found for quercetin or luteolin. It would seem that the AH effects of tilia PCs were slightly more enhanced, especially at lower concentrations, by quercetin or luteolin than the AH effects of PDs. A likely explanation could be that PCs offer more scope for enhancement as they tend to have weaker AH activities than PDs ([@bib7; @bib26]).

The mechanism(s) of the observed synergistic actions are not yet known. Tannins have been shown to deform nematode surfaces ([@bib13; @bib35; @bib36]). Interestingly, exposure to extracts from tannin-containing plants (which would also have contained flavonoids) caused degeneration of muscle cells, accumulation of electron-dense vesicles and marked intracellular disorganisation ([@bib8]). These authors speculated whether the lesions observed in the cuticle of ensheathed L3 larvae could have been due to accumulation of metabolic products, which in turn could have produced cellular toxicity by blocking the metabolic exchange with the environment. Quercetin is well known as an inhibitor of P-glycoproteins (P-gp), which play an important role in the transport of xenobiotics ([@bib19]). It is not known whether this activity also contributes to enhancing the anthelmintic effects of tannins.

To summarise, this study provided the first evidence of synergistic effects between condensed tannins and two common flavonoids, quercetin and luteolin, in terms of inhibiting the *in vitro* exsheathment of *H. concortus* L3 larvae. Some of the synergistic effects occurred at lower PC than PD concentrations, perhaps implying that the generally weaker AH activity of PCs can benefit more from the addition of quercetin. These findings suggest that opportunities should be investigated for increasing anthelmintic activity by mixing plant materials that contain condensed tannins and quercetin or luteolin flavonoids, or to select plants with enhanced tannin and quercetin or luteolin contents.

Conflicts of interest {#sec5}
=====================

The authors declared that there is no conflict of interest.

Supplementary data {#appsec1}
==================

The following is the supplementary data related to this article:

C. Klongsiriwet is grateful to the Royal Thai Government for a PhD scholarship. Hildred PYO-Farm (Goring-on-Thames, Reading, U.K.) kindly provided leaves from black and red currant bushes and Mr and Mrs D. and A. Prudence (Goring-on-Thames, Oxfordshire, U.K.) provided the goat willow (*Salix caprea*) leaves. J. Quijada is grateful for financial support from the European Commission (Marie Curie Initial Training Network, PITN-GA-2011-289377, "LegumePlus").

Supplementary data related to this article can be found at [http://dx.doi.org/10.1016/j.ijpddr.2015.06.001](10.1016/j.ijpddr.2015.06.001){#intref0010}.

![Structures of condensed tannins, flavonoids (flavan-3-ols, luteolin, quercetin, naringenin and taxifolin) and other polyphenols (arbutin and vanillic acid).](gr1){#fig1}

![Larval exsheathment of *Haemonchus contortus* in the presence of different condensed tannin types (at 300 μg fraction/ml), which were isolated from tilia flowers, goat willow leaves, black currant leaves and red currant leaves, and quercetin or luteolin (30 and 60 μM) and their combinations (CT = condensed tannins; PC = procyanidin tannins; PD = prodelphinidin tannins).](gr2){#fig2}

![Synergistic effects between condensed tannins (CT) and quercetin (60 and 30 μM) on the inhibition of *Haemonchus contortus* larval exsheathment after 60 min.](gr3){#fig3}

![Synergistic effects between condensed tannins (CT) and luteolin (60 and 30 μM) on the inhibition of *Haemonchus contortus* larval exsheathment after 60 min.](gr4){#fig4}

###### 

Composition of condensed tannins (CT) isolated from tilia flowers and leaves of goat willow, black currant and red currant.

  Plant source    CT fraction    CT-content (g CT/100 g fraction)   mDP[a](#tbl1fna){ref-type="table-fn"}   PC[b](#tbl1fnb){ref-type="table-fn"} %   PD[b](#tbl1fnb){ref-type="table-fn"} %   *cis*[c](#tbl1fnc){ref-type="table-fn"}*%*   *trans*[c](#tbl1fnc){ref-type="table-fn"}*%*
  --------------- -------------- ---------------------------------- --------------------------------------- ---------------------------------------- ---------------------------------------- -------------------------------------------- ----------------------------------------------
  Goat willow     F1             70.4 (±1.7)                        2.26 (0.0)                              96.2 (0.0)                               3.8                                      6.0 (0.0)                                    94.0
  F2              97.3 (±2.9)    6.35 (0.1)                         95.7 (0.0)                              4.3                                      8.9 (0.2)                                91.1                                         
  Tilia           F1             52.5 (±1.5)                        2.89 (0.0)                              100 (0.0)                                0.0                                      88.6 (0.1)                                   11.4
  F2              90.8 (±10.6)   8.80 (0.0)                         100 (0.0)                               0.0                                      95.4 (0.1)                               4.6                                          
  Black currant   F1             66.4 (±0.4)                        2.78 (0.0)                              7.1 (2.4)                                92.9                                     22.0 (0.2)                                   78.0
  F2              99.8 (±2.4)    9.67 (0.1)                         5.3 (0.0)                               94.7                                     17.8 (0.1)                               82.2                                         
  Red currant     F1             43.4 (±3.8)                        5.41 (0.1)                              10.1 (0.0)                               89.9                                     62.5 (0.5)                                   37.5
  F2              88.9 (±11.9)   19.0 (0.3)                         5.1 (0.0)                               94.9                                     74.6 (0.2)                               25.4                                         

mDP: mean degree of polymerisation.

PC, PD: percentage of procyanidin or prodelphinidin tannins.

*cis, trans*: percentage of *cis*- or *trans*-flavan-3-ols.

###### 

The 50% effective concentrations of tannin fractions (F1 and F2) and two flavonoids when tested *in vitro* in the exsheathment inhibition assay of *Haemonchus contortus* third stage larvae with either the Juan or INRA strains and 50% effective concentrations of CT plus two flavonoids (EC~50~-values; μg of CT or flavonoid/ml) at 30 and 60 μM concentrations on *H. contortus* third stage larvae INRA strain.

  Plant source or compound   CT fraction              EC~50~ values (μg/ml) (95% CI)                                                                                                    
  -------------------------- ------------------------ -------------------------------- ------------------------ ---------------------- ---------------------- ------------------------- ----
  **PC rich fractions**                                                                                                                                                                 
  Goat willow                F1                       299 (273--324)^Y^                --                       --                     --                     --                        --
  F2                         176 (140--222)^r,W^      394 (366--423)^b,s,U^            103 (72.3--149)^a,C^     --                     --                     --                        
  Tilia                      F1                       142 (109--199)^U,V^              --                       --                     --                     --                        --
  F2                         140 (116--168)^r,U^      356 (298--423)^b,s,T^            62.4 (42.8--90.1)^a,A^   156 (81.6--338)^c,A^   \<37.5                 75.9 (47.5--116)^a,d,A^   
  **PD rich fractions**                                                                                                                                                                 
  Black currant              F1                       178 (162--196)^W,X^              --                       --                     --                     --                        --
  F2                         60.1 (28.8--90.1)^r,R^   277 (234--338)^b,s,S^            137 (89.3--232)^a,D^     164 (129--210)^c,A^    31.2 (10.9--52.0)^d^   170 (129--230)^c,e,B^     
  Red currant                F1                       95.3 (71.4--126)^T^              --                       --                     --                     --                        --
  F2                         75.8 (54.3--99.0)^r,S^   126 (101--155)^b,s,R^            78.8 (56.7--110)^a,B^    --                     --                     --                        
  **Luteolin**                                        \<71.5                           17.1 (13.8--20.2)        --                     --                     --                        --
  **Quercetin**                                       \<75.5                           21.0 (19.2--23.0)        --                     --                     --                        --

^a,b,c,d,e,r,s,A,B,C,D,R,S,T,U,V,W,X^: Lower case letters are used to indicate significant differences within rows and upper case letters within columns (P \< 0.05).

^r,s,R,S,T,U,V,W,X^ These letters indicate significant differences between the EC~50~-values of Juan and INRA strains (experiments are based on tannin fractions) (P \< 0.05).

^a,b,c,d,A,B,C,D^ These letters indicate significant differences between EC-~50~ value of the tannin-flavonoid combination experiments with the INRA strain (P \< 0.05).
