Quantification of DNA and RNA with absorption and fluorescence spectroscopy.
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in cell and molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques-Hoescht 33258 dye binding, ethidium bromide binding are described. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.