1. Introduction {#s0005}
===============

In changing climatic scenario, plants often face periods of soil and atmospheric water deficits and/or salinity stress because of scarcity of water availability and deteriorating quality across the globe. As per the available reports, 32--84% poor quality ground water is used for irrigation in different states of India ([@b0180], [@b0100]). Simultaneously, lack of precipitation, high rate of evapo-transpiration and un-sustainable use of water resources could also lead to drought and salinity problems in lesser productive arid and semi-arid regions. In response to abiotic stresses, plants use their in-built ability to adjust in stressful environment by altering various physiological, biochemical, cellular and molecular processes that eventually improve their growth and development.

Pearl millet \[*Pennisetum glaucum* (L.) R. Br. emend. Stuntz.\]-wheat (*Triticum aestivum* L.) is an important cropping system of the water scarce, salt affected, marginal lands underlain with salty groundwater in semi-arid regions. Moreover, pearl millet is highly vigorous, quick growing and relatively more drought and salinity tolerant major cereal crop after barley ([@b0125]). Whereas, wheat is second most important food crop, which is consumed approximately by 36% of the population and considered as semi tolerant crop with a salt tolerance limit of 6 dSm^−1^ ([@b0065], [@b0245]). However, under deficit irrigation with saline water, the production losses owing to different environmental stresses is a major concern to cope with rising food needs ([@b0250]). Application of plant bioregulators (PBRs) could play a crucial role in improving crop growth, development and productivity ([@b0195]). Among many PBRs examined to enhance the stress tolerance, salicylic acid (SA) and thiourea (TU) are widely used in different crops under variety of stresses controlled conditions ([@b0275]). SA is an important phytohormone that regulates a wide range of metabolic and physiological reactions in plants like cell growth, germination, seedling establishment, respiration, responses to abiotic stresses ([@b0105]), water stress in wheat ([@b0025]) and salinity and water stresses in barley ([@b0085]). Whereas Thiourea (TU) is a synthetic PBR having 36% nitrogen and 42% sulfur that gained importance in plant stress tolerance. Therefore, the present study was conducted at field scale to evaluate the stress mitigation effect of salicylic acid and thiourea for increasing production of pearl millet-wheat cropping system.

2. Materials and methods {#s0010}
========================

2.1. Experimental details {#s02000}
-------------------------

The field study on pearl millet-wheat was conducted during 2016--17 and 2017--18 at ICAR-CSSRI experimental farm located at Nain village, Panipat having inland salinity and poor quality water ([Table 1](#t0005){ref-type="table"}). The study was conducted in double split plot design with three replications. It comprised of 36 treatment combinations i.e. three irrigation regimes based on crop evapo-transpiration (ET~c~; calculated using FAO Penman-Monteith method with already established crop coefficients) ([@b0080]) viz., irrigation water equivalent to 100% (I~1~), 80 (I~2~) and 60 (I~3~)of crop ET at critical growth stages in the main plots and four saline irrigation water treatments (by mixing available saline groundwater) having 2, 4, 8 and 12 dS m^−1^ electrical conductivity (EC) in sub plots and application of two plant bioregulators - viz., salicylic acid (SA) and thiourea (TU) along with control (without PBRs) in sub sub plots. Salicylic acid (SA) and Thiourea (TU) were applied through seed priming and twice in foliar spray. Seed priming was done with 1 mM salicylic acid (SA) and 500 ppm thiourea (TU) at sowing and as foliar sprays at specified critical growth stages in both crops (based on concentration standardized in laboratory germination test). In case of pearl millet, bioregulators were applied at vegetative (21 days after emergence) and flowering stages (55--60 DAS) and in wheat at booting (60--70 DAS) and milking stages (90--95 DAS) with equal amount of normal distilled water in control. All agronomic management practices i.e. fertilizer, irrigation, weed and insect pest were uniformly followed as per recommendation for the two crops in the area.Table 1Physico-chemical properties of the experimental field soil and composition of irrigation water.Soil propertiesSoil depth (cm)0--1515--3030--6060--90pHs7.43 ± 0.907.59 ± 1.117.62 ± 1.337.65 ± 0.57~ECe\ (dS\ m~^-1^~)~7.61 ± 2.329.28 ± 2.1510.23 ± 2.2610.03 ± 2.14Organic carbon (g kg^−1^)4.67 ± 0.622.67 ± 0.441.77 ± 0.301.58 ± 0.25Available Nitrogen (N) kg ha^−1^127.81 ± 14.04115.50 ± 10.25107.46 ± 10.698.6 ± 6.57Available Phosphorus (P) kg ha^−1^33.77 ± 4.2827.80 ± 6.8726.00 ± 4.7123.90 ± 4.39Available Potassium (K) kg ha^−1^209.40 ± 10.59203.53 ± 9.01201.35 ± 5.77196.10 ± 8.11Composition of irrigation water2 dS m^−1^4 dS m^−1^8 dS m^−1^12 dS m^−1^EC~iw~(dS m^−1^)1.914.108.1012.10pH~iw~7.787.797.917.95[^1]

2.2. Plant material and observation recorded {#s0015}
--------------------------------------------

Pearl millet variety-HHB-146 and wheat variety KRL 210 recommended for salinity and drought prone areas of north western plain zones (NWPZ) of India were used ([@b0140]). Plant height (cm) of randomly selected plants and grain yield (t ha^−1^) of net treatment plot were recorded at harvest. While for physiological and biochemical traits, the leaf sample from randomly selected and tagged plants, were collected after 14 days of foliar spray at reproductive stage of respective crops, quickly sealed in humified polythene bags, maintained at low temperature in ice filled boxes and transported to the laboratory. Relative water content (RWC%) was calculated by [@b0305], chlorophyll content (mg g^−1^ FW) was assessed using [@b0095] by dimethyl sulfoxide (DMSO) calculation according to [@bib326]. Membrane injury (MI %) estimated with [@b0075], chlorophyll fluorescence (Fv/Fm) was recorded by dark adaptation method ([@b0325]) using fluorescence meter (Mini PAM, Walz) after adapting the leaf in dark for 25 min by using leaf clip on the tagged plant in the field. [@b0040] method was used for proline content (mg g^−1^ FW) estimation using 3% sulphosalicylic acid. Lipid peroxidation was recorded in terms of MDA (malondialdehyde) content using trichloroacetic acid and TBA (thiobarbituric acid) with the help of [@b0090]. An extinction coefficient 155 mM^−1^ cm^−1^ was taken for MDA content (nmole g^−1^ FW) calculations.

2.3. Statistical analysis {#s0020}
-------------------------

All the recorded data were analysed using analysis of variance (ANOVA) technique (SAS) for split split plot design using SAS 9.2 software ([@b0235]) and pair wise comparisons were made using Tukey's test at *p* ≤ 0.05.

3. Results {#s0025}
==========

In pearl millet, significantly greater height was recorded during 2017 (202.08 cm) in comparison to 2016 (185.56 cm), but there were no significant differences due to various deficit irrigation regimes ([Table 2](#t0010){ref-type="table"}). Plant height decreased significantly with increasing salinity stress except under S~1~ (4 dS m^−1^) which had acquired the maximum height among all the salinity levels (199.61 cm). Application of both SA (8.4%) and TU (7.2%) significantly increased plant height over control, but statistically equivalent to each other ([Table 2](#t0010){ref-type="table"}). In case of wheat, the year 2016--17 recorded significantly taller plants (93.41 cm) than the year 2017--18 (91.22 cm). The plant height decreased significantly with increasing each level of drought (I~1~ to I~3~) and salinity (S~0~ to S~3~) stress ([Table 2](#t0010){ref-type="table"}). There was significant increase in plant height with application of SA (10.8%) and TU (13.7%) over control, but magnitude of the increment was significantly higher in TU (97.07 cm) over SA. Interaction effect of deficit irrigation regimes and differential irrigation water salinity brought significant differences in height of both the crops. Integration of S~1~ + I~3~ recorded taller plant height (205.82 cm) than combinations of I~1~ with all salinity levels except S~2~ + I~2~, S~2~ + I~3~ and S~3~ with all the irrigation water regimes in pearl millet. In wheat, the association of G~2~ (TU) + S~0~ significantly increased plant height as compared to the most of remaining combinations of treatments. S~0~ with I~3~ resulted in significantly higher plant height as compared to rest other combinations except S~1~ + I~3~.Table 2Effect of deficit irrigation regimes, differential irrigation water salinity and plant bioregulators on physiological traits.Pearl milletWheatTreatments/ Studied traitsPH (cm)RWC (%)TC(mgg^−1^)Fv/FmPH(cm)RWC (%)TC(mgg^−1^)Fv/FmYears2016/2016--17185.56^B^77.48^B^1.61^B^0.60593.41^A^72.87^A^2.150.652017/2017--2018202.08^A^80.40^A^1.70^A^0.61991.22^B^70.77^B^2.130.64SEd±2.330.560.040.0190.780.120.060.01CD (*P* = 0.05)5.201.250.09NS1.740.27NSNSDeficit irrigation regimesI~1~ (100% ET)193.1279.421.710.61695.64^A^72.39^A^2.35^A^0.68^A^I~2~ (80% ET)194.5078.711.660.60792.12^B^71.91^B^2.13^B^0.65^B^I~3~ (60% ET)193.8478.691.600.61289.19^C^71.17^C^1.94^C^0.62^C^SEd±2.860.690.050.0160.960.150.070.01CD (*P* = 0.05)NSNSNSNS2.130.340.170.02Differential salinity levelsS~0~ (2 dS m^−1^)198.27^A^84.89^A^1.77^A^0.666^A^102.50^A^75.18^A^2.36^A^0.69^A^S~1~ (4 dS m^−1^)199.61^A^84.09^A^1.73^A^0.623^B^97.98^B^73.83^AB^2.28^A^0.67^A^S~2~ (8 dS m^−1^)191.86^B^76.19^B^1.60^B^0.609^B^89.28^C^71.95^B^2.07^B^0.64^B^S~3~ (12 dS m^−1^)185.53^C^70.59^C^1.52^B^0.549^C^79.51^D^66.32^C^1.85^C^0.60^C^SEd±2.941.150.050.0131.321.100.080.01CD (*P* = 0.05)5.812.270.090.0272.622.170.150.02PBRsG~0~ (control)184.17^B^74.94^B^1.48^B^0.61785.35^C^69.48^B^1.94^B^0.62^B^G~1~ (1 mM SA)199.73^A^80.79^A^1.74^A^0.60794.53^B^72.08^A^2.21^A^0.66^A^G*~2~*(500 ppm TU)197.56^A^81.08^A^1.75^A^0.61297.07^A^73.91^A^2.27^A^0.67^A^SEd±2.540.990.040.0141.140.950.070.01CD (*P* = 0.05)5.031.970.08NS2.261.880.130.02[^2]

RWC is a measurement of cellular and tissue hydration caused by cellular water deficit. Both the crops showed similar trends for decrease or increase in RWC except the study years i.e. pearl millet showed higher values during 2017 (80.40%), while wheat during 2016 (72.87%). In both crops, RWC decreased with increasing level of drought that was statistically significant in case of wheat. The RWC decreased significantly at 8 dSm^−1^ and further at 12 dSm^−1^ salinity stresses ([Table 2](#t0010){ref-type="table"}). Significantly higher RWC was recorded i.e. 80.79 and 81.08% in pearl millet and 72.08 and 73.91% in wheat, respectively by the application of SA and TU ([Table 2](#t0010){ref-type="table"}). Interaction effects of deficit irrigation regimes, irrigation water salinity and PBRs were found significant in both the crops. Chlorophyll content was also affected by study years, deficit irrigation regimes, varying levels of irrigation water salinity and PBRs. In case of pearl millet, deficit irrigation caused non-significant reduction in the chlorophyll content, whereas, it was significantly lower at EC~iw~ 8 and 12 dS m^-1^salinity as compared to 4 dS m^−1^ and control (S~0~). Under salinity, the chlorophyll content was reduced by 9.6 and 14.12% at EC~iw~ 8 and 12 dS m^−1^, respectively in comparison to control ([Table 2](#t0010){ref-type="table"}). In case of wheat, similar trends of reduction in chlorophyll content were noted, but there was no significant effect of the study years, whereas each level of deficit irrigation significantly reduced the chlorophyll content. Both the applied PBRs significantly enhanced total chlorophyll content in both crops over control ([Table 2](#t0010){ref-type="table"}). SA and TU were statistically equivalent with corresponding values of 1.74 and 1.75 mg g^−1^ in pearl millet and 2.21 and 2.27 mg g^−1^ in wheat, respectively. There was no significant effect of studied years, irrigation regimes and PBRs on Fv/Fm (chlorophyll fluorescence) in pearl millet, except for salinity stress, where the Fv/Fm decreased significantly with increasing irrigation water salinity. In case of wheat, the Fv/Fm values decreased significantly with deficit irrigation (0.68 at 100% and 0.62 at 60% ET~c~) and salinity (0.69 at EC~iw~ \~ 2 dS m^−1^ and 0.60 at EC~iw~ \~ 12 dS m^−1^). PBRs were found beneficial and attained significantly higher Fv/Fm values i.e. 0.66 with SA and 0.67 with TU as compared to without PBRs (0.62).

Different salinity levels, PBRs and years of study had significant effect on membrane injury in pearl millet leaves, but remained unaffected by deficit irrigation regimes ([Table 3](#t0015){ref-type="table"}). Higher membrane injury was observed during the year 2016 (35.02%) as compared to (23.21%) in 2017. With increasing irrigation water salinity from S~0~ (2 dS m^−1^) to S~3~ (12 dS m^−1^), progressive increase in membrane injury (25% to 33.34% in pearl millet and 42.77% to 46.99% in wheat) was recorded. A significant reduction (\~8% in pearl millet and 2--3% in wheat) in membrane injury were recorded under SA and TU application as compared to control ([Table 3](#t0015){ref-type="table"}). Deficit irrigation did not cause any significant effect on proline accumulation in pearl millet while salinity resulted in significantly higher accumulation of proline i.e. 1.85 mg g^−1^ FW at EC~iw~ \~ 4 dS m^−1^, 2.57 mg g^−1^ FW at EC~iw~ \~ 8 dS m^−1^ and 3.07 mg g^−1^ FW at EC~iw~ \~ 12 dS m^−1^ in comparison to control (1.63 mg g^−1^ FW at EC~iw~ \~ 2 dS m^−1^). Both the PBRs had brought significant decrement in proline accumulation (20.97% reduction by SA and 28.99% reduction by TU) in comparison to untreated plots ([Table 3](#t0015){ref-type="table"}). In case of wheat, study years didn't show any effect on proline accumulation. Wheat subjected to harsher environment under I~3~ (ET~c~ -- 60%) irrigated plots had attained significantly higher value of proline (2.68 mg g^−1^) as compared to I~2~ (2.44 mg g^−1^) and I~1~ (2.18 mg g^−1^). With each increasing salinity level, proline accumulation had significantly increased. The highest and lowest values were obtained under S~3~ (2.81 mg g^−1^) and S~0~ (2.11 mg g^−1^), respectively ([Table 3](#t0015){ref-type="table"}). Both applied PBRs had resulted into lower values of proline. Further, TU (2.24 mg g^−1^) had significantly lower proline accumulation as compared to SA (2.41 mg g^−1^). Interactive effect of years × irrigation regimes × salinity levels × PBRs was found significant on proline accumulation. In pearl millet the maximum accumulation was got in 2016 with I~2~ + S~3~ (4.03 mg g^−1^) in comparison to all other combinations except same salinity under I~1~ (3.83 mg g^−1^) in the same year. In wheat combination of lowest salinity level with and no PBRs application maintained the lower values of proline as compared to others. Combination of 2017--18 + S~3~ (12 dS m^−1^) + G~0~ (Control) resulted into the highest value (3.46 mg g^−1^) as compared to rest others ([Table 3](#t0015){ref-type="table"}).Table 3Effect of deficit irrigation regimes, deferential irrigation water salinity and plant bioregulators on biochemical traits.Treatments/ Studied traitsPearl milletWheatYearsMI (%)Proline (mg g^−1^ FW)LP (nmole g^−1^ FWMI (%)Proline (mg g^−1^ FW)LP (nmole g^−1^ FW2016/2016--1735.02^A^2.49^A^6.85^A^43.65^B^2.322.34^B^2017/2017--201823.21^B^2.08^B^5.57^B^45.58^A^2.552.46^A^SEd±0.500.090.160.390.100.03CD (*P* = 0.05)1.110.190.370.88NS0.07Deficit irrigation regimesI~1~ (100% ET)28.462.186.1443.29^B^2.18^C^2.38I~2~ (80% ET)29.182.326.2244.75^A^2.44^B^2.39I~3~ (60% ET)29.712.356.2645.80^A^2.68^A^2.43SEd±0.610.100.200.480.120.04CD (*P* = 0.05)NSNSNS1.070.28NSDifferential salinity levelsS~0~ (2 dS m^−1^)25.00^D^1.63^D^5.43^C^42.77^C^2.11^D^2.25^B^S~1~ (4 dS m^−1^)27.79^C^1.85^C^5.57^C^43.82^BC^2.31^C^2.29^B^S~2~ (8 dS m^−1^)30.33^B^2.57^B^6.72^B^44.87^B^2.51^B^2.45^A^S~3~ (12 dS m^−1^)33.34^A^3.07^A^7.10^A^46.99^A^2.81^A^2.45^A^SEd±0.840.100.190.640.070.06CD (*P* = 0.05)1.660.190.371.260.130.12PBRsG~0~ (control)34.63^A^2.67^A^7.41^A^46.06^A^2.65^A^2.40G~1~ (1 mM SA)26.61^B^2.11^B^5.60^B^44.09^B^2.41^B^2.40G~2~(500 ppm TU)26.10^B^2.07^B^5.61^B^43.70^B^2.24^C^2.40SEd±0.730.080.160.550.060.05CD (*P* = 0.05)1.440.170.321.090.12NS[^3]

Lipid peroxidation (LP) did not show any significant effect of deficit irrigation in both the crops while salinity stress led to a significant increase in the content of MDA in both the crops i.e. 30.75% increase in MDA content in pearl millet and 8.88% in wheat at EC~iw~ \~ 12 dS m^−1^ ([Table 3](#t0015){ref-type="table"}). Application of PBRs (SA and TU) was found to reduce oxidative damage in pearl millet by reducing MDA content by 24% over control (no PBRs) but such effect was not observed in wheat ([Table 3](#t0015){ref-type="table"}).

Na^+^/K^+^ and Na^+^/Ca^2+^ ratio are indicators of stress tolerance in plants. The study years did not cause any significant variation in Na:K ratio in pearl millet. Among varying irrigation regimes, higher Na:K ratio was obtained in I~2~ (80% ET~c~) (0.48) irrigation regime over other regimes ([Table 4](#t0020){ref-type="table"}). The Na:K ratio was significantly increased by each progressive increase in salinity level and the uppermost value (0.57) was obtained under S~3~ (12 dS m^−1^). There was significant reduction in Na:K ratio by employing the PBRs (SA and TU; 0.40) as compared to control (0.60).Table 4Effect of deficit irrigation regimes, deferential irrigation water salinity and plant bioregulators on Na:K, Na:Ca and grain yield.Treatments/ Studied traitsPearl milletWheatNa:KNa:CaGrain Yield(t ha^−1^)Na:KNa:CaGrain Yield(t ha^−1^)Years2016/2016--170.462.95 ^A^2.11^B^0.371.914.63^A^2017/2017--20180.462.60^B^2.43^A^0.392.004.25^B^SEd±0.010.090.020.010.100.04CD (*P* = 0.05)NS0.200.05NSNS0.09Deficit irrigation regimesI~1~ (100% ET)0.452.762.34^A^0.371.854.60^A^I~2~ (80% ET)0.482.852.25^B^0.381.954.42^B^I~3~ (60% ET)0.472.732.22^B^0.402.074.29^C^SEd±0.010.110.030.010.120.05CD (*P* = 0.05)NSNS0.06NSNS0.12Differential salinity levelsS~0~ (2 dS m^−1^)0.38^D^2.44^C^2.35^A^0.30^D^1.59^C^4.95^A^S~1~ (4 dS m^−1^)0.43^C^2.57 ^BC^2.37^A^0.33^C^1.72^C^4.92^A^S~2~ (8 dS m^−1^)0.47^B^2.89^B^2.29^B^0.39^B^2.03^B^4.57^B^S~3~ (12 dS m^−1^)0.57^A^3.30 ^A^2.07^C^0.50^A^2.55 ^A^3.32^C^SEd±0.010.090.020.010.090.07CD (*P* = 0.05)0.020.180.050.020.190.14PBRsG~0~ (control)0.60^A^3.80 ^A^2.08^B^0.45^A^2.01 ^A^4.03^C^G~1~ (1 mM SA)0.40^B^2.37^B^2.38^A^0.36^B^1.74^B^4.55^B^G~2~(500 ppm TU)0.40^B^2.38^B^2.35^A^0.33^C^1.66^B^4.73^A^SEd±0.010.080.020.010.070.06CD (*P* = 0.05)0.020.160.040.020.150.12[^4]

The study years and irrigation regimes had no significant effect on Na:K raio in wheat, although, the lowest and highest ratios were recorded under I~3~ (0.40) and I~1~ (0.37), respectively. Application of saline water irrigation resulted into significant increase in Na:K ratios with increase in each level of salinity ([Table 4](#t0020){ref-type="table"}). Use of plant bioregulators significantly reduced the ratio over control (0.45), further, the reducing effect of TU (0.33) was significantly better over SA (0.36) Interaction effects of deficit irrigation, saline irrigation water and growth regulators were also found significant. In pearl millet significant interaction effect of deficit irrigation regimes and saline irrigation water was noticed on Na: K ratio of grain and the significantly higher ratio were obtained by S~3~ (12 dS m^−1^) under I~2~ and I~3~ regimes (respective value, 0.64 and 0.63). Combined effect of saline irrigation water and PBRs was noticed significant on Na: K ratio of grain and the maximum value was observed under S~3~ + G~0~ (0.79) which remained at par with S~2~ + G~0~ combination (0.68) only. In wheat, the group of S~3~ (12 dS m^−1^) + G~0~ (Control) + I~1~ (60% ET) \[0.98\] was recorded higher values as compared to other combinations of these treatments except S~3~ (12 dS m^−1^) + G~1~ (SA) + I~2~ (80% ET) \[0.83\] and S~3~ (12 dS m^−1^) + G~0~ (Control) + I~2~ (80% ET) \[0.97\].

In case of pearl millet, lower Na:Ca ratio (2.60) was recorded in year 2017 while for wheat in 2016--17 (1.91). Irrigation regimes had no significant effect on Na:Ca ratio in both the crops ([Table 4](#t0020){ref-type="table"}). From EC~iw~ 4 dSm^−1^ onwards, a significant increment was observed in Na:Ca ratio in both the crops and the extents of increase were 5.33, 18.44 and 35.25% in pearl millet and 8.18, 27.67 and 60.38% in wheat under S~1~, S~2~ and S~3~, respectively over S~0~ ([Table 4](#t0020){ref-type="table"}). PBR\'s application significantly reduced the Na:Ca ratio by 37.6 and 37.4% in pearl millet and 13.43 and 17.41% in wheat by SA and TU, respectively ([Table 4](#t0020){ref-type="table"}).

Pearl millet grain yield was significantly higher (15.16%) in year 2017 (2.43 t ha^−1^) as compared to 2016 (2.11 t ha^−1^). Under different irrigation regimes, the grain yield was significantly higher (2.34 t ha^−1^) in irrigation level I~1~ (100% ET) compared to deficit irrigations at I~2~ (80% ET) (2.25 t ha^−1^) and I~3~ (60% ET) (2.22 t ha^−1^). Among different salinity levels, grain yield declined significantly by 2.55% (2.29 t ha^−1^) at S~2~ (8 dS m^−1^) and further by 11.91% (2.07 t ha^−1^) under subsequent higher salinity S~3~ (12 dS m^−1^) level as compared to S~0~ (\~2 dS m^−1^; 2.35 t ha^−1^) except S~1~ (4 dS m^−1^; 2.37 t ha^−1^) with at par yield ([Table 4](#t0020){ref-type="table"}). Application of SA and TU resulted in significant increase in grain yield i.e. 14.42 (2.38 t ha^−1^) and 12.98% (2.35 t ha^−1^), respectively over the G~0~ (2.08 t ha^−1^). However there was no significant difference between both PBRs. In wheat ([Table 4](#t0020){ref-type="table"}), significantly higher grain yield (4.63 t ha^−1^) was obtained in 2016--17 as compared to 2017--18 (4.25 t ha^−1^). Deficit irrigation regimes caused significant reduction in grain yield (p \< 0.05) i.e. 3.91% under I~2~ (80% ET~c~) and 6.74% under I~3~ (60% ET~c~).

Among different irrigation water salinity levels, S~0~ (4.95 t ha^−1^) saline water has recorded the highest grain yield that was equivalent to S~1~. The grain yield was significantly reduced at 8 dS m^−1^ (S~2~; 4.57 t ha^−1^) and subsequently at S~3~ (3.32 t ha^−1^). The application of both PBRs (SA and TU) significantly improved the wheat grain yield over control. The application of TU (4.73 t ha^−1^) proved significantly better than SA (4.55 t ha^−1^) in increasing wheat grain yields.

Co-relation analysis

The association of different traits with grain yield under the effect of deficit irrigation using different salinity water irrigation and use of PBRs was assessed by correlation analysis. There were significant changes in the studied traits with the application of both the PBRs in relation with grain yield ([Fig. 1](#f0005){ref-type="fig"}). Both the PBRs showed effectiveness in mitigating the adverse effect of stresses in the two crops, but SA was found more effective for pearl millet while TU proved relatively better for wheat.Fig. 1Pearson's correlation coefficients for association using SPSS v19 among different physiological and biochemical traits with grain yield in pearl millet and wheat.

4. Discussion {#s0030}
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Pearl millet--wheat is an important cropping system covering around 2.26 million ha arid and semi-arid tracts in India having water deficit and osmotic stress conditions ([@b0315], [@b0145]). Survival of plants, in stressful environments by manipulating growth, physiological, biochemical and molecular responses; is an important indicator of their ability to overcome multiple and simultaneous stresses ([@b0160]).

Plant response to environmental stresses is fundamentally analysed by growth analysis. In both pearl millet and wheat, decline in plant height in response to salinity and drought, occurs mainly due to decrease in osmotic potential of the soil solution in presence of excess soluble salts. Decrease in osmotic potential of soil solution causes ion imbalance and toxicity by hindering water and nutrients uptake in plants ([@b0135], [@b0320]). Stress either due to drought or salinity is known to decrease plant water uptake ability that leads to reduced plant growth and impaired metabolic processes by the accumulation of toxic ions ([@b0140]). In this study, both the crops showed decrease in RWC under stress conditions. But the application of SA and TU maintained the hydration of cells up to an optimal level, under stress conditions, through accumulation of osmolytes, which sustained water uptake and increased RWC of tissues ([@b0205]). Decrease in chlorophyll content under water deficit and salinity stress either in pearl millet or in wheat could be attributed to the rate of degradation of chlorophyll or due to reduction in activity of chlorophyll biosynthesis enzymes ([@b0265], [@b0170]). Further aggravated stress symptoms have also been observed with greater reductions in chlorophyll concentration ([@b0140]). Chlorophyll is an important molecule associated with photosynthesis, and these plant bio-regulators by maintaining cellular osmoticum helps in improving the chl a and b contents ([@b0045], [@b0240]). Generally Fv/Fm reflects the maximum efficiency of the light absorbption and its conversion into chemical energy which is considered as the most powerful eco-physiological tool to study the photosynthetic process in plants ([@b0175], [@b0185]). In the present study, Fv/Fm declined under the effect of abiotic stresses either individual or combined, might be due to the disturbances in the integrity of membrane and function of thylakoids in chloroplasts ([@b0290]). Increased gas exchange attributes due to application of salicylic acid and thiourea might be helpful in the improvement of the photosynthetic performance and chlorophyll florescence enabling plant to tolerate environmental stresses ([@b0120], [@b0295], [@b0205]).

Cell membrane stability is an established index to evaluate crop plants against abiotic stress tolerance ([@b0135]). Significant changes were noted on MI in both pearl millet and wheat under drought and salinity. Abiotic stresses particularly drought and salinity enhances the degree of membrane fatty acids saturation by changing the properties of proteins that ultimately increased permeability of plasma membrane ([@b0060], [@b0215], [@b0210]). Salicylic acid and thiourea have been found to mitigate the negative effect of stresses on MI through the activation of anti-oxidative defense machinery that is connected with the active oxygen species accumulation and plays a vital role in maintaining redox state of membrane proteins ([@b0220], [@b0030]).

Plants generally protect themselves against abiotic stresses by the osmolytes accumulation and their elevation is attributed to a stress tolerance mechanism ([@b0270]). Proline an important stress indicator stabilizes and protects macromolecules against the severities of ROS by improving osmotic adjustments ([@b0150], [@b0015]). Results of present study recorded higher proline content with increased intensity of stressesin both the crops. The results also indicated significant participation of SA and TU in maintaining cell turgor, boosting nitrogen assimilation and decline in proline oxidase activity as suggested earlier ([@b0055], [@b0115], [@b0205]). Increased proline regulated water potential in plant cells enhanced the plant defense under various abiotic stresses ([@b0155], [@b0255]). Abiotic stress induced ROS caused alteration of cell membrane produced by oxidation of acids of the lipid bi-layer ([@b0050]). In this study also MDA content increased in pearl millet and wheat under saline conditions, whereas deficit irrigations had little effect. Higher MDA level might induce more leakage of electrolytes from cells and increased H~2~O~2~ accumulation ([@b0130]), as a consequence of lipid per-oxidation has long been taken as an indicator of stress tolerance. The application of SA and TU might helped in maintenance of fatty acid breakdown, also led to enhanced activity of antioxidant enzymes and resulted in MDA content decrease ([@b0010], [@b0205]).

Stresses increased Na^+^/K^+^ in both the crops, that might ascribe to K^+^ and Na^+^ competition at the plasma membrane, K^+^ transport inhibition in xylem tissues and/or Na^+^ induced K^+^ efflux from the roots ([@b0165], [@b0140]). Salinity caused significant increase in the ratio of Na^+^/Ca^2+^ in both the crops. Reduced Ca^2+^ uptake under stress environments induced reduction in the binding of Ca^2+^ to the plasma membrane ([@b0225], [@b0260]), resulting in a change in membrane permeability that can be detected as leakage of K^+^ from the cell ([@b0070]). PBRs i.e. SA and TU were found effective in reducing the Na^+^/K^+^ and Na^+^/Ca^2+^ ratio in pearl millet and wheat. Besides accumulating compatible solutes like proline and sugars, these PBRs also helped in elevating the level of K^+^ (a major compatible inorganic solute) in plant cell that have an osmo-protectant function to protect plants during osmotic and/or ionic stresses ([@b0205], [@b0255]).

About 15-16% more grain yield of pearl millet was recorded in study year 2017 than 2016 may be due to 29 mm higher rainfall in 2017. In case of wheat, grain yield was found more in study year 2016--17 than 2017--18 ([Table 4](#t0020){ref-type="table"}). During 2016--17 cropping season, rainfall received (93.30 mm) was 23.10 mm (32.9%) higher than same period in 2017--18 (70.2 mm). This helped to leach down more of the excess soluble salt added with saline water irrigation below root zone and also nullified the effect of moisture deficit treatments. In addition, temperature and moisture conditions during vegetative phase and relatively dryer weather conditions during grain development resulted in better growth and productivity may be another reason for higher yields in first year. Enhanced grain and biomass yields and yield attributes of crops due to congenial climatic conditions also reported earlier ([@b0285]). Imposed matric stress i.e. from I~1~ regime to I~2~ and further I~3~ decreased the pearl millet and wheat yield (3.91 and 6.74%) ([Table 4](#t0020){ref-type="table"}) are in agreement with finding of [@b0020], who reported 6.83 and 41.98% average reduction in wheat grain yield under 80 and 60% ET~c~ based deficit irrigations. Imposed deficit irrigation (80 and 60% ET~c~) reduced wheat yield due to lowering of water potential, reduction in photosynthesis, decrease in leaf expansion, impaired photosynthetic machinery, premature leaf senescence as well as due to a reduction in the assimilate partitioning and activities of sucrose and starch synthesis enzymes ([@b0280], [@b0300]). Under saline irrigation of 8 (S~2~) and 12 dS m^−1^ (S~3~), pearl millet and wheat yield declined by 2.55, 7.68 and 11.91, 32.93%, respectively, compared to 2 dS m^−1^ (S~0~) treatment. The decrease in yield under salinity might be due to high osmotic stress caused ruptured cell membrane, reduced osmotic potential, decreased photosynthesis, protein synthesis and translocation/mobilization of nutrient/food materials from plant parts to grain that ultimately reduced dry matter partitioning in source to sink and declined yield of pearl millet and wheat ([@b0140]). These facts also supported that plant under salinity stress faces many abnormalities in physiological, morphological and biochemical processes ([@b0190]), and lead to reduced yield. Reduction in grain yield due to increasing irrigation water salinity could be due to higher Na^+^ and lower K^+^ content in plant. Pearl millet and wheat yield was significantly enhanced by priming and spraying both of bioregulators (SA and TU) as compared to control (G~0~). The application of TU found superior in case of wheat that increased 3.96 and 17.36% grain yield as compared to SA and control, respectively. [@b0035] found increased wheat grain yield by tune of 24.39% by spraying 0.75 mM SA. Mobilization of dry matter (reserves)/sucrose from leaves to grains via effects on phloem loading increased in wheat with TU spray at tiller stage. Salicylic acid, a phenolic phytohormone, regulates the growth and development of plants, photosynthesis, transpiration, ion absorption and transport ([@b0110]), and thus its application has shown positive responses in mitigating drought ([@bib327]) and osmotic stress ([@bib328]). Salicylic acid also has immense role in enhancing root system and thus can rectify the salt induced growth and production inhibition and greater biomass.

5. Conclusion {#s0035}
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Plant height and grain yield reduction were recorded with increasing moisture and salinity stresses in both pearl millet and wheat. Abiotic stresses specially drought and salinity were mitigated by seed priming and foliar spray of salicylic acid and thiourea at critical growth stages of both the crops. The increasing counteractive effect of PRBs to salt stress was observed with increasing level of salinity. SA and TU use in both crops improved RWC and TC and recovered MI, proline, sodium concentration, lipid peroxidation. TU proved more effective in wheat but SA performed better for pearl millet that too in mild salinity. Thus, the two PBRs can be recommended, after the large scale field testing and standardization of their economic spray schedules, for improving the pearl millet-wheat crop performance under marginal quality irrigation conditions.

Declaration of Competing Interest
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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgement {#s0045}
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This research was supported by grants from the University Grants Commission Fellowship, India 2016 (F./201516/ NFO-2015-17OBC-RAJ-38714/(SAIII/Website). The authors are thankful to Director, NDRI and Director, CSSRI, Karnal for providing necessary facilities for the research work.

Author contribution {#s0050}
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R.K.Y. conceived and developed the work. T. Y. conducted the experiments and analysed the results. A.K., R.K.Y. and R. K. participated to data discussion and critically revised the manuscript. T.Y., A.K., G.Y. and M.K. wrote the manuscript. All authors read and approved the final manuscript.

Peer review under responsibility of King Saud University.

[^1]: Data of soil properties are expressed as the means ± standard deviation of 108 experimental plots. Characters of pH~s~ and pH~iw~- pH of soil saturation paste and irrigation water, respectively; EC~e~ and EC~iw~- Electrical conductivity of soil saturation paste extract and irrigation water, respectively.

[^2]: Data presented are means ± S.E.M of three independent replications. Two-way ANOVA followed by Tukey's test was used for analysis. Different letters denote significant differences at p \< 0.05. PH -- plant height; RWC -- Relative water content; TC- Total chlorophyll; Fv/Fm- Fluorescence variable/ Fluorescence maximum.

[^3]: Data presented are means ± S.E.M of three independent replications. Two-way ANOVA followed by Tukey's test was used for analysis. Different letters denote significant differences at p \< 0.05. MI -- membrane injury; LP -- Lipid peroxidation.

[^4]: Data presented are means ± S.E.M of three independent replications. Two-way ANOVA followed by Tukey's test was used for analysis. Different letters denote significant differences at p \< 0.05. Na:K- Sodium: Potassium; Na:Ca -- Sodium:Calcium.
