[Separation of ionic currents responsible for action potential generation in isolated neurons of frog spinal ganglia].
The technique was developed for isolating neurons from the frog spinal ganglia. The isolated cells completely preserved electrical excitability and were investigated under intracellular dialysis conditions. Fast and slow inward current components were found. They were separated by TTX and cadmium: the fast inward current component was blocked by TTX, while the slow inward current was blocked by cadmium. Slow inward current diminished in Na-free solution and calcium ions are supposed to participate in its transfer.