The barramundi is a catadromous and hermaphrodite fish that inhabits coastal waters. The commercial and recreational fishing activities put this fish under anthropic pressure. Previous genetic studies have shown a strong regional structure. However, they used scarce sampling and only few markers (mtDNA & microsatellites). Only little is know about the small geographic and individual scale in that species. The aim of the project is to provide a fine-scale genetic study focusing on Van Diemen gulf area using a RAD-seq approach to understand the biology and population connectivity of barramundi.