Sample size calculation was performed with GraphPadInstat program GraphPad Software Inc., San Diego, CA, USA.
El cálculo del tamaño de la muestra se hizo con el programa GraphPadInstat GraphPad Software Inc., San Diego, CA, EE. UU..
O cálculo do tamanho da amostra foi feito com o programa GraphPadInstat GraphPad Software Inc., San Diego, CA, EUA.