Next, the membrane was incubated overnight at 4°C with anti-NCX1 rat monoclonal antibodies 1:1500 dilution, Swant® Swiss antibodies, Bellinzona, CH, SWI, anti-SERCA2 rat monoclonal antibodies 1:2500 dilution, Abcam Inc., MA, USA, anti-PLB rat monoclonal antibodies 0.25 µg/ml, Upstate Biotechnology, Lake Placid, NY, USA or anti-phospho-Ser-PLB rat monoclonal antibodies 1:1000, Upstate Biotechnology, Lake Placid, NY, USA.
Enseguida, la membrana fue incubada durante la noche a 4 ºC con anticuerpos monoclonales de Rata anti-NCX1 dilución 1:1500, anticuerpos suizos Swant, Bellinzona, CH, SWI, anticuerpos monoclonales de Rata anti-SERCA2 dilución 1:2.500, Abcam Inc., MA, EUA, anticuerpos monoclonales de Rata anti-PLB 0,25 mg/mL, Upstate Biotechnology, Lake Placid, NY, EUA o anticuerpos monoclonales anti-fosfo-Ser-PLB de Rata 1:1000, Upstate Biotechnology, Lake Placid, NY, EUA.
Em seguida, a membrana foi incubada durante a noite a 4 °C com anticorpos monoclonais de camundongo anti-NCX1 diluição 1:1500, anticorpos suíços Swant®, Bellinzona, CH, SWI, anticorpos monoclonais de camundongo anti-SERCA2 diluição 1:2.500, Abcam Inc., MA, EUA, anticorpos monoclonais de camundongo anti-PLB 0,25 mg/mL, Upstate Biotechnology, Lake Placid, NY, EUA ou anticorpos monoclonais anti-fosfo-Ser-PLB de camundongo 1:1000, Upstate Biotechnology, Lake Placid, NY, EUA.