Aliquots of RT reaction containing 2.0 µg of cDNA were added to a mixture containing TaqMan Universal PCR Master Mix Applied Biosystems and custom assay containing sense and anti-sense primers and Taqman probe Applied Biosystems, Foster City, CA, USA specific for each gene, and the volume completed to 25ml with water treated with DEPC.
Alícuotas de la reacción de RT conteniendo 2,0 µg de cADN fueron adicionadas a una mezcla conteniendo TaqMan Universal PCR Master Mix Applied Biosystems y ensayo customizado conteniendo primers "sense" y "anti-sense" y sonda Taqman Applied Biosystems, Foster City, CA,USA específicos para cada gen, y el volumen completado para 25 ml con agua tratada con DEPC.
Alíquotas da reação de RT contendo 2,0 µg de cDNA foram adicionadas a uma mistura contendo TaqMan Universal PCR Master Mix Applied Biosystems e ensaio customizado contendo primers "sense" e "anti-sense" e sonda Taqman Applied Biosystems, Foster City, CA, USA específicos para cada gene, e o volume completado para 25 ml com água tratada com DEPC.