***TASK*** the task is to summarize an input biomedical literature in six sentences 
    ***INPUT*** the input is a biomedical literature 
    ***OUTPUT*** the output is the summary of an input biomedical literature in six sentences 
    ***DOCUMENTATION*** 
    ***EXAMPLES*** 
    Input: Two trials were conducted to investigate the individual and combined effects of in-ovo injection of creatine monohydrate ( CMH : ) and glucose on the somatic characteristics and energy status of broiler embryos and hatchlings and the growth performance of chicks during the first wk posthatch . In trial 1 , 550 17.5-day-old living , fertile eggs were r and omly assigned to 1 of 5 treatment groups , including 1 ) a noninjected control , 2 ) a 0.4 mL diluent-injected control , or 0.4 mL diluents containing 3 ) 6 mg CMH , 4 ) 25 mg glucose , or 5 ) a combination of 6 mg CMH and 25 mg glucose ( identified as CMH+G : ) . In trial 2 , after hatch , 48 hatchlings with individual body weight ( BW : ) close to the average BW of their incubation group were r and omly selected and placed in 6 replicates of 8 birds each to evaluate their growth performance during the first wk posthatch . Compared with the noninjected and the 0.4-mL diluent-injected control groups , individual injection of CMH or glucose did not affect the hatching time , hatchability , somatic characteristics , or concentrations of glycogen and glucose in the liver and pectoralis major ( PM : ) muscle , although injection of CMH increased ( P < 0.05 ) concentrations of creatine ( CR : ) and phosphocreatine ( PCR : ) in the PM muscle on d 19 of incubation ( E19.5 ) . However , the CMH+G treatment increased ( P < 0.05 ) BW relative to set egg weight ( SEW : ) on E19.5 , as well as residual yolk sac weight on the day of hatch . Moreover , the CMH+G treatment also increased ( P < 0.05 ) concentrations of glycogen and glucose in the liver and concentrations of Cr and PCr in the PM muscle on E19.5 . Chicks in the CMH+G group also exhibited higher BW gain during the first wk than controls ( P < 0.05 ) . These results indicate that combined injection of CMH and glucose during the last stage of incubation have a synergistic effect on improvement of the energy status of embryos and hatchlings , which is useful for enhancing embryo development , and subsequently improving chick growth during the early posthatch stages 
    Output:
    
Nevertheless , in general , in ovo feeding of carbohydrates decreases the hatch rate , improves the hatch weight , but it does not seem to influence the post-hatch performance of broilers  .
The inoculation of 75 mg of glucose in the albumen seems to bring better results .