human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: Cancer therapy with rapamycin has been successfully implemented for kidney cancer , glioblastoma and prostate cancer . However , there are few studies concerning the effects of rapamycin on the treatment of human melanoma . In this study , we investigated whether rapamycin may be a promising strategy for the effective treatment of melanoma and explored the possible mechanism for this by culturing M14 cells in vitro and treating with rapamycin at three concentrations ( 10 , 50 or 100 nmol/l ) . MDC and LC3B staining , western blot analysis , flow cytometry and transmission electron microscopy were employed . We revealed that rapamycin induced autophagy and inhibited the proliferation of M14 cells in a concentration-dependent manner , Furthermore , western blot analysis revealed an upregulated expression of Bcl-2 and downregulated expression of Bax in M14 cells . In conclusion , rapamycin induced autophagy and inhibited the growth of M14 cells . The mechanism may involve regulation of the expression of Bcl-2 family proteins . Rapamycin appears to be a promising strategy for the effective treatment of melanoma .
    OUTPUT:

agent
resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: Cancer therapy with rapamycin has been successfully implemented for kidney cancer , glioblastoma and prostate cancer . However , there are few studies concerning the effects of rapamycin on the treatment of human melanoma . In this study , we investigated whether rapamycin may be a promising strategy for the effective treatment of melanoma and explored the possible mechanism for this by culturing M14 cells in vitro and treating with rapamycin at three concentrations ( 10 , 50 or 100 nmol/l ) . MDC and LC3B staining , western blot analysis , flow cytometry and transmission electron microscopy were employed . We revealed that rapamycin induced autophagy and inhibited the proliferation of M14 cells in a concentration-dependent manner , Furthermore , western blot analysis revealed an upregulated expression of Bcl-2 and downregulated expression of Bax in M14 cells . In conclusion , rapamycin induced autophagy and inhibited the growth of M14 cells . The mechanism may involve regulation of the expression of Bcl-2 family proteins . Rapamycin appears to be a promising strategy for the effective treatment of melanoma .
    OUTPUT:
