human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Gadd45a , the first well-defined p53 downstream gene , can be induced by multiple DNA-damaging agents , which plays important roles in the control of cell cycle checkpoint , DNA repair process and signaling transduction . Our previous findings suggested that Gadd45a maintains cell-cell adhesion and cell contact inhibition . However , little is known about how Gadd45a participates in the suppression of malignancy in human cancer cells . To examine the functions of Gadd45a in cell invasion and metastasis , we performed the adhesion , wound-healing and transwell assays in Gadd45a ( +/+ ) and Gadd45a ( -/- ) MEF cell lines . We found the adhesion , migration and invasive abilities were much higher in Gadd45a deficient cells . We furthermore applied high-throughput cDNA microarray analysis and bioinformatics analysis to analyze the mechanisms of Gadd45a gene in invasion and metastasis . Compared with the Gadd45a wild type cells , the Gadd45a deficient cells showed a wide range of transcripts alterations . The altered gene pathways were predicted by the MAS software , which indicated focal adhesion,cell communication,ECM-receptor interaction as the three main pathways . Real-time PCR was employed to validate the differentially expressed genes . Interestingly , we figured out that the deregulations of these genes are caused neither by genomic aberrations nor methylation status . These findings provided a novel insight that Gadd45a may involve in tumor progression by regulating related genes expressions .
    OUTPUT:

agent
activating invasion and metastasis
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: Gadd45a , the first well-defined p53 downstream gene , can be induced by multiple DNA-damaging agents , which plays important roles in the control of cell cycle checkpoint , DNA repair process and signaling transduction . Our previous findings suggested that Gadd45a maintains cell-cell adhesion and cell contact inhibition . However , little is known about how Gadd45a participates in the suppression of malignancy in human cancer cells . To examine the functions of Gadd45a in cell invasion and metastasis , we performed the adhesion , wound-healing and transwell assays in Gadd45a ( +/+ ) and Gadd45a ( -/- ) MEF cell lines . We found the adhesion , migration and invasive abilities were much higher in Gadd45a deficient cells . We furthermore applied high-throughput cDNA microarray analysis and bioinformatics analysis to analyze the mechanisms of Gadd45a gene in invasion and metastasis . Compared with the Gadd45a wild type cells , the Gadd45a deficient cells showed a wide range of transcripts alterations . The altered gene pathways were predicted by the MAS software , which indicated focal adhesion,cell communication,ECM-receptor interaction as the three main pathways . Real-time PCR was employed to validate the differentially expressed genes . Interestingly , we figured out that the deregulations of these genes are caused neither by genomic aberrations nor methylation status . These findings provided a novel insight that Gadd45a may involve in tumor progression by regulating related genes expressions .
    OUTPUT:
