human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: AIM To develop a simple , fast and inexpensive approach as well as an instrument for detection of gene mutation . METHODS Pyrosequencing based on bioluminometry assay was employed to detect gene mutation . Pyrosequencing is a method of sequencing by synthesis step-by-step using four enzymes , DNA-polymerase , ATP sulfurylase , luciferase and apyrase . The signal was produced by detecting pyrophosphate released during a dNTP incorporation . For mutation detection , a DNA fragment was amplified by PCR at first , followed by a single-stranded DNA preparation . In the second step , a short primer was annealed to the position just before the mutation point . Finally , specific dNTPs were added in terms of the template sequence . The mutation species can be readily determined by the sequence . RESULTS A new instrument was developed for gene mutation detection by pyrosequencing . To iteratively inject small amount of each dNTP for the sequencing reaction , capillaries were used to connect dNTP reservoirs and the reaction chamber . Each dNTP was delivered by adding a gas pressure on the top of a corresponding dNTP reservoir , by which 0.2 microL of dNTP can be exactly added each time . It was theoretically proved that undesired liquid seep through the capillary did not affect the sequencing reactions in pyrosequencing . In addition , the three possible variants ( wildtype , mutant and heterozygote ) of a mutant point Cys275Ser in P53 gene exon 8 were determined by pyrosequencing using the instrument . A simple method was also described for rapidly distinguishing the type of a variant . CONCLUSION The developed method is very simple , and the corresponding instrument is inexpensive and easy to operate , which can be used to detect many types of mutation .
    OUTPUT:

agent
genomic instability and mutation
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: AIM To develop a simple , fast and inexpensive approach as well as an instrument for detection of gene mutation . METHODS Pyrosequencing based on bioluminometry assay was employed to detect gene mutation . Pyrosequencing is a method of sequencing by synthesis step-by-step using four enzymes , DNA-polymerase , ATP sulfurylase , luciferase and apyrase . The signal was produced by detecting pyrophosphate released during a dNTP incorporation . For mutation detection , a DNA fragment was amplified by PCR at first , followed by a single-stranded DNA preparation . In the second step , a short primer was annealed to the position just before the mutation point . Finally , specific dNTPs were added in terms of the template sequence . The mutation species can be readily determined by the sequence . RESULTS A new instrument was developed for gene mutation detection by pyrosequencing . To iteratively inject small amount of each dNTP for the sequencing reaction , capillaries were used to connect dNTP reservoirs and the reaction chamber . Each dNTP was delivered by adding a gas pressure on the top of a corresponding dNTP reservoir , by which 0.2 microL of dNTP can be exactly added each time . It was theoretically proved that undesired liquid seep through the capillary did not affect the sequencing reactions in pyrosequencing . In addition , the three possible variants ( wildtype , mutant and heterozygote ) of a mutant point Cys275Ser in P53 gene exon 8 were determined by pyrosequencing using the instrument . A simple method was also described for rapidly distinguishing the type of a variant . CONCLUSION The developed method is very simple , and the corresponding instrument is inexpensive and easy to operate , which can be used to detect many types of mutation .
    OUTPUT:
