human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Pancreatic cancer remains the fourth most common cause of cancer-related death in the United States . Potent therapeutic strategies are urgently needed for pancreatic cancer . Cucurmosin is a novel type1 ribosome-inactivating protein ( RIP ) isolated from the sarcocarp of Cucurbita moschata ( pumpkin ) . Due to its cytotoxicity , cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells , but the specific mechanism is still unclear . We explored the function of cucurmosin in BxPC-3 pancreatic cancer cells using multiple cellular and molecular approaches such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay , flow cytometry , reverse transcription polymerase chain reaction ( RT-PCR ) , Western blotting and transmission electron microscopy for observing typical changes and formation of apoptotic bodies . We found that cucurmosin inhibited the proliferation of BxPC-3 cells in a time- and dose-dependent manner , and increased the cell population in the G0-G1 phase . With increasing concentration of cucurmosin , the expression of EGFR , p-PI3K , Akt , p-Akt , mTOR , p-mTOR , P70S6K-Î± , p-P70S6K-Î± , 4E-BP1 and p-4E-BP1 at the protein level was decreased , whereas the expression of p-Bad and caspase-9 was elevated . However , the mRNA expression of EGFR did not change . These findings suggest that cucurmosin can down-regulate the expression of EGFR by targeting . Cucurmosin induces the apoptosis of BxPC-3 pancreatic cancer cells via the PI3K/Akt/mTOR signaling pathway .
    OUTPUT:

agent
sustaining proliferative signaling;resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Pancreatic cancer remains the fourth most common cause of cancer-related death in the United States . Potent therapeutic strategies are urgently needed for pancreatic cancer . Cucurmosin is a novel type1 ribosome-inactivating protein ( RIP ) isolated from the sarcocarp of Cucurbita moschata ( pumpkin ) . Due to its cytotoxicity , cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells , but the specific mechanism is still unclear . We explored the function of cucurmosin in BxPC-3 pancreatic cancer cells using multiple cellular and molecular approaches such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay , flow cytometry , reverse transcription polymerase chain reaction ( RT-PCR ) , Western blotting and transmission electron microscopy for observing typical changes and formation of apoptotic bodies . We found that cucurmosin inhibited the proliferation of BxPC-3 cells in a time- and dose-dependent manner , and increased the cell population in the G0-G1 phase . With increasing concentration of cucurmosin , the expression of EGFR , p-PI3K , Akt , p-Akt , mTOR , p-mTOR , P70S6K-Î± , p-P70S6K-Î± , 4E-BP1 and p-4E-BP1 at the protein level was decreased , whereas the expression of p-Bad and caspase-9 was elevated . However , the mRNA expression of EGFR did not change . These findings suggest that cucurmosin can down-regulate the expression of EGFR by targeting . Cucurmosin induces the apoptosis of BxPC-3 pancreatic cancer cells via the PI3K/Akt/mTOR signaling pathway .
    OUTPUT:
