human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: BACKGROUND The importance of cell-surface nucleolin in cancer biology was recently highlighted by studies showing that ligands of nucleolin play critical role in tumorigenesis and angiogenesis . By using a specific antagonist that binds the C-terminal tail of nucleolin , the HB-19 pseudopeptide , we recently reported that HB-19 treatment markedly suppressed the progression of established human breast tumor cell xenografts in the athymic nude mice without apparent toxicity . METHODS The in vivo antitumoral action of HB-19 treatment was assessed on the spontaneous development of melanoma in the RET transgenic mouse model . Ten days old RET mice were treated with HB-19 in a prophylactic setting that extended 300 days . In parallel , the molecular basis for the action of HB-19 was investigated on a melanoma cell line ( called TIII ) derived from a cutaneous nodule of a RET mouse . RESULTS HB-19 treatment of RET mice caused a significant delay in the onset of cutaneous tumors , several-months delay in the incidence of large tumors , a lower frequency of cutaneous nodules , and a reduction of visceral metastatic nodules while displaying no toxicity to normal tissue . Moreover , microvessel density was significantly reduced in tumors recovered from HB-19 treated mice compared to corresponding controls . Studies on the melanoma-derived tumor cells demonstrated that HB-19 treatment of TIII cells could restore contact inhibition , impair anchorage-independent growth , and reduce their tumorigenic potential in mice . Moreover , HB-19 treatment caused selective down regulation of transcripts coding matrix metalloproteinase 2 and 9 , and tumor necrosis factor-alpha in the TIII cells and in melanoma tumors of RET mice . CONCLUSIONS Although HB-19 treatment failed to prevent the development of spontaneous melanoma in the RET mice , it delayed for several months the onset and frequency of cutaneous tumors , and exerted a significant inhibitory effect on visceral metastasis . Consequently , HB-19 could provide a novel therapeutic agent by itself or as an adjuvant therapy in association with current therapeutic interventions on a virulent cancer like melanoma .
    OUTPUT:

agent
inducing angiogenesis;evading growth suppressors;activating invasion and metastasis
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: BACKGROUND The importance of cell-surface nucleolin in cancer biology was recently highlighted by studies showing that ligands of nucleolin play critical role in tumorigenesis and angiogenesis . By using a specific antagonist that binds the C-terminal tail of nucleolin , the HB-19 pseudopeptide , we recently reported that HB-19 treatment markedly suppressed the progression of established human breast tumor cell xenografts in the athymic nude mice without apparent toxicity . METHODS The in vivo antitumoral action of HB-19 treatment was assessed on the spontaneous development of melanoma in the RET transgenic mouse model . Ten days old RET mice were treated with HB-19 in a prophylactic setting that extended 300 days . In parallel , the molecular basis for the action of HB-19 was investigated on a melanoma cell line ( called TIII ) derived from a cutaneous nodule of a RET mouse . RESULTS HB-19 treatment of RET mice caused a significant delay in the onset of cutaneous tumors , several-months delay in the incidence of large tumors , a lower frequency of cutaneous nodules , and a reduction of visceral metastatic nodules while displaying no toxicity to normal tissue . Moreover , microvessel density was significantly reduced in tumors recovered from HB-19 treated mice compared to corresponding controls . Studies on the melanoma-derived tumor cells demonstrated that HB-19 treatment of TIII cells could restore contact inhibition , impair anchorage-independent growth , and reduce their tumorigenic potential in mice . Moreover , HB-19 treatment caused selective down regulation of transcripts coding matrix metalloproteinase 2 and 9 , and tumor necrosis factor-alpha in the TIII cells and in melanoma tumors of RET mice . CONCLUSIONS Although HB-19 treatment failed to prevent the development of spontaneous melanoma in the RET mice , it delayed for several months the onset and frequency of cutaneous tumors , and exerted a significant inhibitory effect on visceral metastasis . Consequently , HB-19 could provide a novel therapeutic agent by itself or as an adjuvant therapy in association with current therapeutic interventions on a virulent cancer like melanoma .
    OUTPUT:
