human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: It has been shown that regulation of EGFR expression in prostate cancer cells is mostly at the transcriptional level. microRNA-133 ( miR-133 ) has long been recognized as a muscle-specific miRNA which may regulate myoblast differentiation and participate in many myogenic diseases . Recently , it has been reported that miR-133 is also involved in other tumors , such as bladder cancer , esophageal cancer and may regulate cell motility in these cancer cells . In the present study , we examined the expression and effects of miR-133 in two hormone-insensitive prostate cancer cell lines . The expression of miR-133a and miR-133b were analyzed by quantitative RT-PCR . After transfection of miR-133a and miR-133b , cell viability assay , luciferase assay , western blot analysis , cell migration and invasion assay were conducted in Du145 and PC3 cells . In this study , we showed that miRâ133a and miR-133b are expressed at the detection limit in two hormone-insensitive prostate cancer cell lines , PC3 and DU145 . Ectopic expression of miR-133 inhibited cell proliferation , migration and invasion in these cells . We also provide the first evidence that miR-133 may target EGFR . Our study provided the first glimpse of the functional role of miR-133 in two hormone-independent prostate cancer cell lines . These results may add to our knowledge on the molecular basis of prostate cancer progression .
    OUTPUT:

agent
activating invasion and metastasis;sustaining proliferative signaling
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: It has been shown that regulation of EGFR expression in prostate cancer cells is mostly at the transcriptional level. microRNA-133 ( miR-133 ) has long been recognized as a muscle-specific miRNA which may regulate myoblast differentiation and participate in many myogenic diseases . Recently , it has been reported that miR-133 is also involved in other tumors , such as bladder cancer , esophageal cancer and may regulate cell motility in these cancer cells . In the present study , we examined the expression and effects of miR-133 in two hormone-insensitive prostate cancer cell lines . The expression of miR-133a and miR-133b were analyzed by quantitative RT-PCR . After transfection of miR-133a and miR-133b , cell viability assay , luciferase assay , western blot analysis , cell migration and invasion assay were conducted in Du145 and PC3 cells . In this study , we showed that miRâ133a and miR-133b are expressed at the detection limit in two hormone-insensitive prostate cancer cell lines , PC3 and DU145 . Ectopic expression of miR-133 inhibited cell proliferation , migration and invasion in these cells . We also provide the first evidence that miR-133 may target EGFR . Our study provided the first glimpse of the functional role of miR-133 in two hormone-independent prostate cancer cell lines . These results may add to our knowledge on the molecular basis of prostate cancer progression .
    OUTPUT:
