human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: OBJECTIVE To study the effect of necrostatin ( Nec-1 ) on apoptosis induced by aluminum ( Al ) , and approach the mechanism . METHODS Neural cell death model was made by 4 mmol/L Al treated neuroblastoma cells ( SH-SY5Y ) . Cell viabilities were detected at different concentrations of Al and/or Nec-1 . Hoechst 33342/PI double staining was used to observe apoptosis and ( or ) necrosis that were quantified by flow cytometry using Annexin V/PI double staining . Apoptotic pathway was tested by activities of Caspase-3 , Caspase-8 and Caspase-9 . In addition , the expression of NF-kappa B and Cyt-c was measured by immunocytochemistry . RESULTS Cell viabilities were significantly decreased with the increasing concentrations of Al ( P &lt ; 0.05 ) , which could be significantly upregulated by 60 micromol/L Nec-1 ( P &lt ; 0.05 ) and were correlated with the concentrations of Nec-1 ( P &lt ; 0.05 , P &lt ; 0.01 ) . Apoptosis and necrosis were observed under fluorescent microscope and quantified by flow cytometry , which suggested an increasing trend of apoptotic and necrotic rates ( P &lt ; 0.05 , P &lt ; 0.01 ) . Whereas , Nec-1 could not only decrease the necrotic rate but also apoptotic rate as well ( P &lt ; 0.05 , P &lt ; 0.01 ) . Data of Nec-1 on caspases activities showed that Nec-1 could not affect Caspase-9 activity ( P &gt ; 0.05 ) and Cty-c protein expression as well ( P &gt ; 0.05 ) . However , Nec-1 could reduce Caspase-8 activity significantly ( P &lt ; 0.05 , P &lt ; 0.01 ) and increase NF-kappa B protein expression ( P &lt ; 0.05 , P &lt ; 0.01 ) and finally decrease Caspase-3 activity ( P &lt ; 0.05 ) . CONCLUSION Nec-1 could reduce cell apoptosis induced by Al , through Caspase-8 pathway , and up-regulate the expression of NF-kappa B protein .
    OUTPUT:

agent
resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: OBJECTIVE To study the effect of necrostatin ( Nec-1 ) on apoptosis induced by aluminum ( Al ) , and approach the mechanism . METHODS Neural cell death model was made by 4 mmol/L Al treated neuroblastoma cells ( SH-SY5Y ) . Cell viabilities were detected at different concentrations of Al and/or Nec-1 . Hoechst 33342/PI double staining was used to observe apoptosis and ( or ) necrosis that were quantified by flow cytometry using Annexin V/PI double staining . Apoptotic pathway was tested by activities of Caspase-3 , Caspase-8 and Caspase-9 . In addition , the expression of NF-kappa B and Cyt-c was measured by immunocytochemistry . RESULTS Cell viabilities were significantly decreased with the increasing concentrations of Al ( P &lt ; 0.05 ) , which could be significantly upregulated by 60 micromol/L Nec-1 ( P &lt ; 0.05 ) and were correlated with the concentrations of Nec-1 ( P &lt ; 0.05 , P &lt ; 0.01 ) . Apoptosis and necrosis were observed under fluorescent microscope and quantified by flow cytometry , which suggested an increasing trend of apoptotic and necrotic rates ( P &lt ; 0.05 , P &lt ; 0.01 ) . Whereas , Nec-1 could not only decrease the necrotic rate but also apoptotic rate as well ( P &lt ; 0.05 , P &lt ; 0.01 ) . Data of Nec-1 on caspases activities showed that Nec-1 could not affect Caspase-9 activity ( P &gt ; 0.05 ) and Cty-c protein expression as well ( P &gt ; 0.05 ) . However , Nec-1 could reduce Caspase-8 activity significantly ( P &lt ; 0.05 , P &lt ; 0.01 ) and increase NF-kappa B protein expression ( P &lt ; 0.05 , P &lt ; 0.01 ) and finally decrease Caspase-3 activity ( P &lt ; 0.05 ) . CONCLUSION Nec-1 could reduce cell apoptosis induced by Al , through Caspase-8 pathway , and up-regulate the expression of NF-kappa B protein .
    OUTPUT:
