human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Certain components of apples have been shown to prevent cancer growth and impede cancer progression . We hypothesized that extracted apple polysaccharides ( APs ) might , therefore , have anticancer effects , through a mechanism involving the induction of apoptosis in cancer cells , partly via the NF-ÎºB pathway . Two human colorectal cancer ( CRC ) cell lines , HT-29 and SW620 , were exposed to different concentrations of APs ( 0.01 , 0.1 or 1 mg/ml ) . Cell apoptosis was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay by flow cytometry and incorporation of 5'-bromodeoxyuridine ( BrdU ) into DNA to identify the proliferating cell fraction , using fluorescence microscopy in vitro . The protein levels of NF-ÎºB/p65 , I-ÎºBÎ± , pI-ÎºBÎ± , Bax , Bcl-xl and Bcl-2 were evaluated by western blotting . The target sites of APs on CRC cells were assessed by flow cytometry . At concentrations of 0.1 and 1 mg/ml , APs showed apoptosis-inducing effects , increased expressions of Bax , nuclear p65 and cytoplasmic pI-ÎºBÎ± , and decreased expressions of Bcl-2 , Bcl-xl and cytoplasmic I-ÎºBÎ± . APs induced apoptosis by slightly activating the NF-ÎºB pathway ; the AP target site could be the Toll-like receptor 4 on the cell membrane . These results demonstrate the potential of APs as agents for clinical prevention and treatment of CRC .
    OUTPUT:

agent
resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: Certain components of apples have been shown to prevent cancer growth and impede cancer progression . We hypothesized that extracted apple polysaccharides ( APs ) might , therefore , have anticancer effects , through a mechanism involving the induction of apoptosis in cancer cells , partly via the NF-ÎºB pathway . Two human colorectal cancer ( CRC ) cell lines , HT-29 and SW620 , were exposed to different concentrations of APs ( 0.01 , 0.1 or 1 mg/ml ) . Cell apoptosis was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay by flow cytometry and incorporation of 5'-bromodeoxyuridine ( BrdU ) into DNA to identify the proliferating cell fraction , using fluorescence microscopy in vitro . The protein levels of NF-ÎºB/p65 , I-ÎºBÎ± , pI-ÎºBÎ± , Bax , Bcl-xl and Bcl-2 were evaluated by western blotting . The target sites of APs on CRC cells were assessed by flow cytometry . At concentrations of 0.1 and 1 mg/ml , APs showed apoptosis-inducing effects , increased expressions of Bax , nuclear p65 and cytoplasmic pI-ÎºBÎ± , and decreased expressions of Bcl-2 , Bcl-xl and cytoplasmic I-ÎºBÎ± . APs induced apoptosis by slightly activating the NF-ÎºB pathway ; the AP target site could be the Toll-like receptor 4 on the cell membrane . These results demonstrate the potential of APs as agents for clinical prevention and treatment of CRC .
    OUTPUT:
