human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Currently there is no good hepatocyte model for studying growth hormone ( GH ) function that reflects its normal physiological roles . Here we report the establishment of a functional hepatocyte cell line , SDRL-1 , from the liver of young male spontaneous dwarf rats ( SDR ) , with isolated GH deficiency . This line has been maintained in Dulbecco's Modified Eagle Medium ( DMEM)/F12 medium supplemented with 10% fetal bovine serum ( FBS ) with retention of a near diploid karyotype for extended periods of time . When grown as a monolayer sheet , it displayed a pavement-like appearance and contact inhibition . These cells have a poorly developed rough endoplasmic reticulum ( r-ER ) , few mitochondria and glycogen granules , and produce a small amount of albumin and Î±-fetoprotein , that is enhanced when grown on a collagen gel sponge . Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner . When the cells were cultured with GH-supplemented medium , the number of mitochondria and glycogen granules increased together with the r-ER and Golgi apparatus . A number of microvilli were observed on the surface of the cultured cells , further suggesting that this cell line is composed of normally functioning hepatocytes . In summary , we established a novel hepatocyte cell line ( SDRL-1 ) , that appears to display normal function , which we propose can serve as a good in vitro model for studying GH-target organ interactions .
    OUTPUT:

agent
evading growth suppressors
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Currently there is no good hepatocyte model for studying growth hormone ( GH ) function that reflects its normal physiological roles . Here we report the establishment of a functional hepatocyte cell line , SDRL-1 , from the liver of young male spontaneous dwarf rats ( SDR ) , with isolated GH deficiency . This line has been maintained in Dulbecco's Modified Eagle Medium ( DMEM)/F12 medium supplemented with 10% fetal bovine serum ( FBS ) with retention of a near diploid karyotype for extended periods of time . When grown as a monolayer sheet , it displayed a pavement-like appearance and contact inhibition . These cells have a poorly developed rough endoplasmic reticulum ( r-ER ) , few mitochondria and glycogen granules , and produce a small amount of albumin and Î±-fetoprotein , that is enhanced when grown on a collagen gel sponge . Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner . When the cells were cultured with GH-supplemented medium , the number of mitochondria and glycogen granules increased together with the r-ER and Golgi apparatus . A number of microvilli were observed on the surface of the cultured cells , further suggesting that this cell line is composed of normally functioning hepatocytes . In summary , we established a novel hepatocyte cell line ( SDRL-1 ) , that appears to display normal function , which we propose can serve as a good in vitro model for studying GH-target organ interactions .
    OUTPUT:
