human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Objective To investigate the effect of bufalin on nucleus-mitochondria localization of human telomerase reverse transcriptase(hTERT) by exploring its effect on proliferation and apoptosis in human esophageal squamous carcinoma EC9706 cells . Methods EC9706 cells were treated with bufalin at various concentrations , and then the cell growth inhibition of EC9706 cells was examined by CCK-8 assay and the 50% inhibitory concentration ( IC(50) ) was calculated.Cell cycle analysis was performed by flow cytometry with PI staining , and nucleus morphology of apoptosis were observed by fluorescence microscopy with Hoechst 33342 staining . The apoptotic index was measured by flow cytometry with Annexin V-FITC/PI double staining. hTERT subcellular localization and protein expression were determined by Western blotting and multiple immunofluorescence labling combined with laser confocal scanning microscopy . Results The proliferation of EC 9706 cells was significantly inhibited by bufalin along with the increase of processing time and concentrations ( p&lt;0.01 ) . After the EC9706 cells were exposed to 100 nmol/L bufalin,the number of cells gradually decreased in G(1) phase and increased in S and G(2)/M phases(p&lt;0.05) . The typical nucleus morphological changes of apoptosis were observed and the apoptotic index was increased(p&lt;0.01) . The expression of hTERT decreased in nucleus but increased in mitochondria(p&lt;0.05) . Conclusions Bufalin can inhibit the proliferation of human esophageal squamous carcinoma EC9706 cells in a time- and dose-dependent manner . It can arrest cell cycle in S and G(2)/M phases and induce the apoptosis of EC 9706 cells. hTERT is localized in both nucleus and mitochondria,and can be partially translocated from nucleus to mitochondria during the bufalin-induced apoptosis .
    OUTPUT:

agent
sustaining proliferative signaling;resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Objective To investigate the effect of bufalin on nucleus-mitochondria localization of human telomerase reverse transcriptase(hTERT) by exploring its effect on proliferation and apoptosis in human esophageal squamous carcinoma EC9706 cells . Methods EC9706 cells were treated with bufalin at various concentrations , and then the cell growth inhibition of EC9706 cells was examined by CCK-8 assay and the 50% inhibitory concentration ( IC(50) ) was calculated.Cell cycle analysis was performed by flow cytometry with PI staining , and nucleus morphology of apoptosis were observed by fluorescence microscopy with Hoechst 33342 staining . The apoptotic index was measured by flow cytometry with Annexin V-FITC/PI double staining. hTERT subcellular localization and protein expression were determined by Western blotting and multiple immunofluorescence labling combined with laser confocal scanning microscopy . Results The proliferation of EC 9706 cells was significantly inhibited by bufalin along with the increase of processing time and concentrations ( p&lt;0.01 ) . After the EC9706 cells were exposed to 100 nmol/L bufalin,the number of cells gradually decreased in G(1) phase and increased in S and G(2)/M phases(p&lt;0.05) . The typical nucleus morphological changes of apoptosis were observed and the apoptotic index was increased(p&lt;0.01) . The expression of hTERT decreased in nucleus but increased in mitochondria(p&lt;0.05) . Conclusions Bufalin can inhibit the proliferation of human esophageal squamous carcinoma EC9706 cells in a time- and dose-dependent manner . It can arrest cell cycle in S and G(2)/M phases and induce the apoptosis of EC 9706 cells. hTERT is localized in both nucleus and mitochondria,and can be partially translocated from nucleus to mitochondria during the bufalin-induced apoptosis .
    OUTPUT:
