human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: The aim of this study was to determine whether isorhamnetin , an immediate 3'-O-methylated metabolite of quercetin , affects proliferation , cell death , and the cell cycle of human colon carcinoma ( HCT-116 ) cells . Isorhamnetin was found to be a potent antiproliferative agent in a dose- and time-dependent manner , with an IC50 of 72 Î¼M after 48 h of incubation as estimated by MTT assay . Flow cytometry and fluorescence microscopy analysis showed that isorhamnetin exerted a stimulatory effect on apoptosis and necrosis . Isorhamnetin also increased the number of cells in G2/M phase . Serum deprivation appeared to potentiate the effects of isorhamnetin on cell death and facilitated cell cycle progression to G0/G1 phase . These results suggest that isorhamnetin might mediate inhibition of HCT-116 cell growth through the perturbation of cell cycle progression and are consistent with the notion that G2/M checkpoints could be a conserved target for flavonoids in human colon cancer cells , leading to apoptotic and necrotic death . These antiproliferative , apoptotic , necrotic , and cell cycle effects suggest that isorhamnetin may have clinically significant therapeutic and chemopreventive capabilities . To our knowledge , this is the first report of the effect of isorhamnetin on human colon cancer cells .
    OUTPUT:

agent
resisting cell death;evading growth suppressors;sustaining proliferative signaling
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: The aim of this study was to determine whether isorhamnetin , an immediate 3'-O-methylated metabolite of quercetin , affects proliferation , cell death , and the cell cycle of human colon carcinoma ( HCT-116 ) cells . Isorhamnetin was found to be a potent antiproliferative agent in a dose- and time-dependent manner , with an IC50 of 72 Î¼M after 48 h of incubation as estimated by MTT assay . Flow cytometry and fluorescence microscopy analysis showed that isorhamnetin exerted a stimulatory effect on apoptosis and necrosis . Isorhamnetin also increased the number of cells in G2/M phase . Serum deprivation appeared to potentiate the effects of isorhamnetin on cell death and facilitated cell cycle progression to G0/G1 phase . These results suggest that isorhamnetin might mediate inhibition of HCT-116 cell growth through the perturbation of cell cycle progression and are consistent with the notion that G2/M checkpoints could be a conserved target for flavonoids in human colon cancer cells , leading to apoptotic and necrotic death . These antiproliferative , apoptotic , necrotic , and cell cycle effects suggest that isorhamnetin may have clinically significant therapeutic and chemopreventive capabilities . To our knowledge , this is the first report of the effect of isorhamnetin on human colon cancer cells .
    OUTPUT:
