human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: BACKGROUND : Although quiescence ( reversible cell cycle arrest ) is a key part in the life history and fate of many mammalian cell types , the mechanisms of gene regulation in quiescent cells are poorly understood . We sought to clarify the role of microRNAs as regulators of the cellular functions of quiescent human fibroblasts . RESULTS : Using microarrays , we discovered that the expression of the majority of profiled microRNAs differed between proliferating and quiescent fibroblasts . Fibroblasts induced into quiescence by contact inhibition or serum starvation had similar microRNA profiles , indicating common changes induced by distinct quiescence signals . By analyzing the gene expression patterns of microRNA target genes with quiescence , we discovered a strong regulatory function for miR-29 , which is downregulated with quiescence . Using microarrays and immunoblotting , we confirmed that miR-29 targets genes encoding collagen and other extracellular matrix proteins and that those target genes are induced in quiescence . In addition , overexpression of miR-29 resulted in more rapid cell cycle re-entry from quiescence . We also found that let-7 and miR-125 were upregulated in quiescent cells . Overexpression of either one alone resulted in slower cell cycle re-entry from quiescence , while the combination of both together slowed cell cycle re-entry even further . CONCLUSIONS : microRNAs regulate key aspects of fibroblast quiescence including the proliferative state of the cells as well as their gene expression profiles , in particular , the induction of extracellular matrix proteins in quiescent fibroblasts .
    OUTPUT:

agent
evading growth suppressors;sustaining proliferative signaling
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: BACKGROUND : Although quiescence ( reversible cell cycle arrest ) is a key part in the life history and fate of many mammalian cell types , the mechanisms of gene regulation in quiescent cells are poorly understood . We sought to clarify the role of microRNAs as regulators of the cellular functions of quiescent human fibroblasts . RESULTS : Using microarrays , we discovered that the expression of the majority of profiled microRNAs differed between proliferating and quiescent fibroblasts . Fibroblasts induced into quiescence by contact inhibition or serum starvation had similar microRNA profiles , indicating common changes induced by distinct quiescence signals . By analyzing the gene expression patterns of microRNA target genes with quiescence , we discovered a strong regulatory function for miR-29 , which is downregulated with quiescence . Using microarrays and immunoblotting , we confirmed that miR-29 targets genes encoding collagen and other extracellular matrix proteins and that those target genes are induced in quiescence . In addition , overexpression of miR-29 resulted in more rapid cell cycle re-entry from quiescence . We also found that let-7 and miR-125 were upregulated in quiescent cells . Overexpression of either one alone resulted in slower cell cycle re-entry from quiescence , while the combination of both together slowed cell cycle re-entry even further . CONCLUSIONS : microRNAs regulate key aspects of fibroblast quiescence including the proliferative state of the cells as well as their gene expression profiles , in particular , the induction of extracellular matrix proteins in quiescent fibroblasts .
    OUTPUT:
