human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: p53 plays an important role in regulating a wide variety of cellular processes , such as cell cycle arrest and/or apoptosis . Dysfunction of p53 is frequently associated with several pathologies , such as cancer and neurodegenerative diseases . In recent years substantial progress has been made in developing novel p53-activating molecules . Importantly , modulation of p53 interaction with its main inhibitor , Mdm2 , has been highlighted as a promising therapy target . In this regard , bimolecular fluorescence complementation ( BiFC ) analysis , by providing direct visualization of protein interactions in living cells , offers a straightforward method to identify potential modulators of protein interactions . In this study , we developed a simple and robust Venus-based BiFC system to screen for modulators of p53-p53 and p53-Mdm2 interactions in live mammalian cells . We used nutlin-3 , a well-known disruptor of p53-Mdm2 interaction , to validate the specificity of the assay . The reduction of BiFC signal mediated by nutlin-3 was correlated with an increase in Puma transactivation , PARP cleavage , and cell death . Finally , this novel BiFC approach was exploited to identify potential modulators of p53-Mdm2 complex formation among a commercially available chemical library of 33 protein phosphatase inhibitors . Our results constitute " proof-of-concept " that this model has strong potential as an alternative to traditional target-based drug discovery strategies . Identification of new modulators of p53-p53 and p53-Mdm2 interactions will be useful to achieve synergistic drug efficacy with currently used anti-tumor therapies .
    OUTPUT:

agent
resisting cell death
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: p53 plays an important role in regulating a wide variety of cellular processes , such as cell cycle arrest and/or apoptosis . Dysfunction of p53 is frequently associated with several pathologies , such as cancer and neurodegenerative diseases . In recent years substantial progress has been made in developing novel p53-activating molecules . Importantly , modulation of p53 interaction with its main inhibitor , Mdm2 , has been highlighted as a promising therapy target . In this regard , bimolecular fluorescence complementation ( BiFC ) analysis , by providing direct visualization of protein interactions in living cells , offers a straightforward method to identify potential modulators of protein interactions . In this study , we developed a simple and robust Venus-based BiFC system to screen for modulators of p53-p53 and p53-Mdm2 interactions in live mammalian cells . We used nutlin-3 , a well-known disruptor of p53-Mdm2 interaction , to validate the specificity of the assay . The reduction of BiFC signal mediated by nutlin-3 was correlated with an increase in Puma transactivation , PARP cleavage , and cell death . Finally , this novel BiFC approach was exploited to identify potential modulators of p53-Mdm2 complex formation among a commercially available chemical library of 33 protein phosphatase inhibitors . Our results constitute " proof-of-concept " that this model has strong potential as an alternative to traditional target-based drug discovery strategies . Identification of new modulators of p53-p53 and p53-Mdm2 interactions will be useful to achieve synergistic drug efficacy with currently used anti-tumor therapies .
    OUTPUT:
