human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: OBJECTIVE To sort and identify side population ( SP ) cancer stem cells ( CSC ) in human prostate cancer ( PCa ) cell lines . METHODS Stem-like cells were isolated from five PCa cell lines Du145 , IA8 , LNCaP , TSU-Pr and PC-3 using FACS based on CD133+ CD44+ immunophenotype and SP in Hoechst staining . The in vitro growth pattern and tumorigenicity of SP stem cells were verified by soft agar colony-formation trial . LNCaP/SP cells were selected for further identification of stem cell properties using immunostaining , proliferation and invasion assay . Eventually , tumorigenicity and metastasis ability of LNCaP/SP were confirmed by xenograft experiments . RESULTS The percentages of CSCs of the CD133 CD44 + immunophenotype were extremely low in the five PCa cell lines . On the contrary , the percentages of the isolated SP cells were significantly higher in Du145 ( [ 0.15 +/- 0.02]% ) , IA8 ( [ 0.60 +/- 0.07 ]% ) , LNCaP ( [ 0.8 +/- 0.1]% ) and TSU-PrL ( [ 2.0 +/- 0.4]% ) , but none was detected in PC-3 . Besides , IA8/SP , LNCaP/SP and TSU-PrL/SP cells showed a significantly greater colony-forming efficiency than non-side population ( NSP ) cells ( P &lt ; 0.05 ) . Compared with LNCaP/NSP cells , LNCaP/SP cells exhibited high expressions of integrin alpha2 , Nanog , CD44 , OCT4 and ABCG2 , remarkably enhanced invasive and proliferative potentials in vitro , and markedly increased tumorigenicity and metastasis ( P &lt ; 0.01 ) . CONCLUSION SP sorting is more suitable than CD133+ CD44+ selection for enriching CSCs from PCa cell lines , and LNCaP/ SP represents a typical CSC population .
    OUTPUT:

agent
activating invasion and metastasis
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: OBJECTIVE To sort and identify side population ( SP ) cancer stem cells ( CSC ) in human prostate cancer ( PCa ) cell lines . METHODS Stem-like cells were isolated from five PCa cell lines Du145 , IA8 , LNCaP , TSU-Pr and PC-3 using FACS based on CD133+ CD44+ immunophenotype and SP in Hoechst staining . The in vitro growth pattern and tumorigenicity of SP stem cells were verified by soft agar colony-formation trial . LNCaP/SP cells were selected for further identification of stem cell properties using immunostaining , proliferation and invasion assay . Eventually , tumorigenicity and metastasis ability of LNCaP/SP were confirmed by xenograft experiments . RESULTS The percentages of CSCs of the CD133 CD44 + immunophenotype were extremely low in the five PCa cell lines . On the contrary , the percentages of the isolated SP cells were significantly higher in Du145 ( [ 0.15 +/- 0.02]% ) , IA8 ( [ 0.60 +/- 0.07 ]% ) , LNCaP ( [ 0.8 +/- 0.1]% ) and TSU-PrL ( [ 2.0 +/- 0.4]% ) , but none was detected in PC-3 . Besides , IA8/SP , LNCaP/SP and TSU-PrL/SP cells showed a significantly greater colony-forming efficiency than non-side population ( NSP ) cells ( P &lt ; 0.05 ) . Compared with LNCaP/NSP cells , LNCaP/SP cells exhibited high expressions of integrin alpha2 , Nanog , CD44 , OCT4 and ABCG2 , remarkably enhanced invasive and proliferative potentials in vitro , and markedly increased tumorigenicity and metastasis ( P &lt ; 0.01 ) . CONCLUSION SP sorting is more suitable than CD133+ CD44+ selection for enriching CSCs from PCa cell lines , and LNCaP/ SP represents a typical CSC population .
    OUTPUT:
