human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: Normal human lung fibroblast diploid cells , WI-38 , become senescent after a definite number of divisions . VA-13 is a line of immortalized cells established by transformation of WI-38 cells by SV40 virus . To determine whether SV40 large T ( SV40-T ) antigen is essential for this immortalization of WI-38 cells we introduced an antisense gene for T antigen into VA-13 . Two morphologically different types of antisense transformant ( VA-AS5-8 and VA-AS37-8 ) were obtained . In both antisense transformants the expression of T antigen was reduced by more than 70% as compared to that in the parent cells . The morphology of the antisense transformants indicated a partial conversion to the senescent phenotype of WI-38 . The relative number of cells in the S phase of the antisense transformants was decreased as compared to that in cultures of VA-13 and about 50% of cells were at G1/0 . The doubling time of the transformants was prolonged to close to the doubling time of WI-38 . The level of expression of retinoblastoma protein ( pRB ) complexed with SV40-T antigen of the antisense transformants was significantly decreased although the level of total pRB was much higher than that in VA-13 . The pRB was present exclusively in the underphosphorylated form . Thus , the decreased level of formation of the complex between SV40-T and pRB or the underphosphorylation of pRB may explain the suppression of growth of antisense transformants . Together , these results show that an antisense gene for SV40-T antigen can efficiently block the cell proliferation and the cell immortalization of VA-13 cells .
    OUTPUT:

agent
enabling replicative immortality;sustaining proliferative signaling
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: Normal human lung fibroblast diploid cells , WI-38 , become senescent after a definite number of divisions . VA-13 is a line of immortalized cells established by transformation of WI-38 cells by SV40 virus . To determine whether SV40 large T ( SV40-T ) antigen is essential for this immortalization of WI-38 cells we introduced an antisense gene for T antigen into VA-13 . Two morphologically different types of antisense transformant ( VA-AS5-8 and VA-AS37-8 ) were obtained . In both antisense transformants the expression of T antigen was reduced by more than 70% as compared to that in the parent cells . The morphology of the antisense transformants indicated a partial conversion to the senescent phenotype of WI-38 . The relative number of cells in the S phase of the antisense transformants was decreased as compared to that in cultures of VA-13 and about 50% of cells were at G1/0 . The doubling time of the transformants was prolonged to close to the doubling time of WI-38 . The level of expression of retinoblastoma protein ( pRB ) complexed with SV40-T antigen of the antisense transformants was significantly decreased although the level of total pRB was much higher than that in VA-13 . The pRB was present exclusively in the underphosphorylated form . Thus , the decreased level of formation of the complex between SV40-T and pRB or the underphosphorylation of pRB may explain the suppression of growth of antisense transformants . Together , these results show that an antisense gene for SV40-T antigen can efficiently block the cell proliferation and the cell immortalization of VA-13 cells .
    OUTPUT:
