human
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
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    INPUT: The functions of Rap-1A in oral carcinogenesis are largely unexplored . In this study , we examined the expression of Rap-1A at different malignant stages of oral cavity squamous cell carcinoma ( OCSCC ) . Semiquantitative RT-PCR , quantitative RT-PCR , and Western blotting were used to evaluate Rap-1A mRNA and protein expressions , respectively , in paired OCSCC patient specimens . To determine the possible correlation between Rap-1A expression and various clinical characteristics , 256 samples from patients with OCSCC were evaluated by immunohistochemical staining . Strong Rap-1A expression was a significant prognostic marker and predictor of aggressive OCSCC . The overall and disease-specific 5-year survival rates were significantly correlated with strong expression of Rap-1A ( P &lt ; 0.001 ) . Functionally , overexpressed Rap-1A could promote oral cancer cell migration and invasion by Transwell chambers and wound healing assay . Conversely , the suppression of Rap-1A expression using Rap-1A-mediated siRNA was sufficient to decrease cell motility . Furthermore , our data also illustrated that Aurora-A could not only induce mRNA and protein expressions of Rap-1A for enhancing cancer cell motility but also co-localize and form a complex with Rap-1A in the oral cancer cell line . Finally , immunohistochemical staining , indirect immunofluorescence , and Western blotting analysis of human aggressive OCSCC specimens revealed a significantly positive correlation between Rap-1A and Aurora-A expression . Taken together , our results suggest that the Aurora-A/Rap-1A pathway is associated with survival , tumor progression , and metastasis of OCSCC patients .
    OUTPUT:

agent
activating invasion and metastasis
The task is to decide the Hallmarks of Cancer (HOC) taxonomy of the article based on its abstract. The input is an abstract text. There are 10 topics you will need to decide whether the article is related to. Topics: sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, activating invasion and metastasis, genomic instability and mutation, tumor promoting inflammation, cellular energetics, avoiding immune destruction. The output should be topics from the above 10 topics, that are related to the input article. Please note one article can be related to multiple topics. Output format: provide a semicolon-separated list of relevant topics.
    ###
    INPUT: The functions of Rap-1A in oral carcinogenesis are largely unexplored . In this study , we examined the expression of Rap-1A at different malignant stages of oral cavity squamous cell carcinoma ( OCSCC ) . Semiquantitative RT-PCR , quantitative RT-PCR , and Western blotting were used to evaluate Rap-1A mRNA and protein expressions , respectively , in paired OCSCC patient specimens . To determine the possible correlation between Rap-1A expression and various clinical characteristics , 256 samples from patients with OCSCC were evaluated by immunohistochemical staining . Strong Rap-1A expression was a significant prognostic marker and predictor of aggressive OCSCC . The overall and disease-specific 5-year survival rates were significantly correlated with strong expression of Rap-1A ( P &lt ; 0.001 ) . Functionally , overexpressed Rap-1A could promote oral cancer cell migration and invasion by Transwell chambers and wound healing assay . Conversely , the suppression of Rap-1A expression using Rap-1A-mediated siRNA was sufficient to decrease cell motility . Furthermore , our data also illustrated that Aurora-A could not only induce mRNA and protein expressions of Rap-1A for enhancing cancer cell motility but also co-localize and form a complex with Rap-1A in the oral cancer cell line . Finally , immunohistochemical staining , indirect immunofluorescence , and Western blotting analysis of human aggressive OCSCC specimens revealed a significantly positive correlation between Rap-1A and Aurora-A expression . Taken together , our results suggest that the Aurora-A/Rap-1A pathway is associated with survival , tumor progression , and metastasis of OCSCC patients .
    OUTPUT:
