In the sentence extracted from biomedical literature, identify all the chemical entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="chemical"> to denote a chemical.

### Input Text: Number and total anionic site surface in the remaining part of the glomerular basement membrane ( lamina densa and lamina rara interna ) were not significantly changed .
### Output Text:
Number and total anionic site surface in the remaining part of the glomerular basement membrane ( lamina densa and lamina rara interna ) were not significantly changed . 