In the sentence extracted from biomedical literature, identify all the chemical entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="chemical"> to denote a chemical.

### Input Text: CONCLUSION : Subacute exposure to PDN induced anxiety and reactivity of microglia .
### Output Text:
CONCLUSION : Subacute exposure to <span class="chemical">PDN</span> induced anxiety and reactivity of microglia . 