In the sentence extracted from biomedical literature, identify all the chemical entities. The required answer format is the same sentence with HTML <span> tags to mark up specific entities. 
### Entity Markup Guides:
Use <span class="chemical"> to denote a chemical.

### Input Text: The expression analysis of Ca ( 2 + ) handling proteins demonstrated that aconitine promoted Ca ( 2 + ) overload through the expression regulation of Ca ( 2 + ) handling proteins .
### Output Text:
The expression analysis of <span class="chemical">Ca</span> ( 2 + ) handling proteins demonstrated that <span class="chemical">aconitine</span> promoted <span class="chemical">Ca</span> ( 2 + ) overload through the expression regulation of <span class="chemical">Ca</span> ( 2 + ) handling proteins . 