External-stimulus-triggered conformational inversion of mechanically self-locked pseudo[1]catenane and gemini-catenanes based on A1/A2-alkyne–azide-difunctionalized pillar[5]arenes

Herein, we report a methodology for constructing mechanically self-locked molecules (MSMs) through the efficient intramolecular copper(i)-catalyzed alkyne–azide cycloaddition (CuAAC) of self-threaded A1/A2-azido-propargyl-difunctionalized pillar[5]arenes. The obtained monomeric “pseudo[1]catenane” and dimeric “gemini-catenane” were isolated and fully characterized using mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, and X-ray crystallography. Upon investigation by 1H NMR spectroscopy in chloroform, the observed motion for the threaded ring in the pseudo[1]catenane was reversibly controlled by the temperature, as demonstrated by variable-temperature 1H NMR studies. Two gemini-catenane stereoisomers were also isolated in which the two pillar[5]arene moieties threaded by two decyl chains were aligned in different topologies. Furthermore, the conformational inversion of pseudo[1]catenane and the gemini-catenanes triggered by solvents and guests was investigated and probed using 1H NMR spectroscopy, isothermal titration calorimetry, and single-crystal X-ray analysis.


Introduction
Mechanically interlocked molecules (MIMs) such as catenanes, rotaxanes, pseudo-rotaxanes, and molecular knots (knotanes) have been extensively synthesized and studied in recent decades, and they have found many applications in the design of molecular machines, chemical probes, molecular catalysts, and drug delivery systems. [1][2][3][4][5][6][7][8][9][10] Macrocyclic molecules such as crown ethers, cyclodextrin, cucurbituril, and calixarene are mainly used in the construction of MIM-based pseudo-rotaxanes, rotaxanes, and catenanes. 11 In addition, pillar[n]arenes have recently attracted considerable interest due to their relative ease of formation, their facile functionalization, and their exceptional ability to selectively encapsulate different kinds of guest molecules. 12,13 Taking advantage of their excellent hostguest properties, the pillar [5]arene-based [2]rotaxane was rst produced through end-capping by the reaction between a diamine axle and a bulky aldehyde stopper; however, the product yield was low. 14 Subsequently, many examples of pillar [5]arene-based [2]rotaxanes were reported based on the improved encapsulation of the axle and an optimized capping procedure. 15 In addition, pillar [5]arene-based [1]rotaxanes in which the rotor and the axle are bound covalently to form a macrocyclic compound were synthesized by capping the selfinclusion axle of the pseudo [1]rotaxane. 15 Moreover, a simple [2] catenane, in which two interlocked macrocycles were present, was prepared from a guest pyridinium derivative bearing alkene groups at both ends followed by a ring-closing metathesis reaction. 16 Mechanically self-locked molecules (MSMs), where one of the covalently linked macrocycles is threaded into the other macrocycle, have been found to offer fascinating molecular architectures and topological features. 17 Pseudo [1]catenanes or MSMs in which the guest alkyl cyclic chain is connected to one pillar [5]arene were initially produced from difunctionalized alkyne groups at the A1/A2 positions of the macrocycle and a 1,12-diazidododecane guest via a copper(I)-catalyzed alkyneazide 1,3-dipolar cycloaddition "click" (CuAAC) reaction. 18 Another example includes the preparation of a pseudo [1]catenane by a reaction between 1,4-dicyanobutane and 1,8-diaminooctane guests (i.e., bearing different lengths of diaminoalkane chains) with difunctionalized amino or carboxy groups present at the A1/A2 positions of the pillar [5]arene, respectively. 19,20 More recently, pseudo [1]catenanes based on the pillar [5]thiacrown and pillar [5]azacrown structures, wherein planar chiral inversion is triggered by a metal cation or acid and is controlled by an anion or a base, have been reported. 21,22 In addition, the chirality switching of planar chiral pseudo [1]catenanes has been reported to be regulated by guest molecules. 23 Since the early discovery of pillararenes, the CuAAC reaction has been employed in their functionalization to construct a wide variety of molecular architectures in high yields. Importantly, this reaction exhibits an excellent functional group tolerance, in addition to being compatible with a wide range of substrates. [24][25][26][27][28][29][30][31][32][33] Thus, we report the synthesis of a pseudo[1]catenane base of self-inclusion A1/A2-asymmetric difunctionalized pillar [5]arenes. In solution, stable pseudo [1]rotaxanes that are formed from the self-inclusion of the straight-chain bromodecyl group on the pillar [5]arene rim, enable the construction of pseudo [1]catenane and gemini-catenane structures via the conversion of bromine to an azide functional group followed by intramolecular CuAAC reaction. The role of external stimuli (i.e., a solvent or guest) in governing the conformational changes from self-included to dethreaded structures via hostguest interactions, which can consequently trigger conformational inversion, are probed by nuclear magnetic resonance (NMR) spectroscopy, isothermal titration calorimetry (ITC), and X-ray single-crystal diffraction. To the best our knowledge, this is the rst report of intramolecular self-locked pseudo [1]catenane and 1 : 1 gemini-catenane structures based on A1/A2asymmetric difunctionalized pillar [5]arenes and their conformational inversion.

Synthesis
Many pseudo [1]rotaxane based pillar[n]arenes have been reported in recent years due to their ability for self-inclusion when a long chain substituent is present on the macrocyclic rim. Recently, we reported the synthesis of a series of A1/A2asymmetric-defunctionalized pillar [5]arenes and their concentration-dependent supramolecular self-assembly behaviors. 34 Based on such systems, precursor compound 1-(10bromodecyloxy)-4-propargyloxybenzene was synthesized via a two-step reaction starting from hydroquinone. An A1/A2asymmetric difunctionalized pillar [5]arene bearing propargyl and bromo functional groups (Pillar-1) was then synthesized by the condensation of an asymmetric hydroquinone derivative and 1,4-dimethoxybenzene in a 1 : 4 ratio in the presence of paraformaldehyde and BF 3 $OEt 2 , as shown in Scheme 1. The azide derivative, Pillar-2, was obtained following the reaction of bromo-derivative Pillar-1 with sodium azide (NaN 3 ) in DMF at room temperature (Scheme 1).
The 1 H NMR spectrum of Pillar-1 (Fig. S6 †) shows a high upeld shi for the methylene proton resonances of the 10bromodecyloxy arm, thereby indicating the successful formation of the pseudo[1]rotaxane. Moreover, the self-inclusion of the long alkyl chain inside the pillar [5]arene cavity was not affected when the 1 H NMR spectrum of Pillar-1 was recorded at a higher temperature of 313 K (Fig. S6 †). To investigate whether the inclusion behavior of the 10-bromodecyloxy chain in the pillar [5]arene cavity involved the self-inclusion of a doublethreaded dimer (i.e., an interpenetration structure) or an nmer, 1 H NMR spectroscopy was carried out at a range of Pillar-1 concentrations (1-64 mM) in chloroform-d at 298 K (Fig. S7 †). The insignicant changes observed in the chemical shi data clearly demonstrate that the behavior of Pillar-1 is concentration independent, suggesting that Pillar-1 exists predominately in its single aggregate form (i.e., the pseudo[1]rotaxane). Further evidence was obtained from two-dimensional diffusion-ordered 1 H NMR (DOSY) experiments. More specically, the DOSY spectra of Pillar-1 at concentrations of 2 and 50 mM revealed a single set of signals, conrming the presence of a single aggregate form in solution. In the case where multiple aggregate sizes exist, a change in the diffusion coefficient (D) would be expected in the DOSY spectrum because larger aggregates possess larger hydrodynamic radii (R), and R is inversely proportional to D according to the Strokes-Einstein equation [D ¼ k B T/(6phR)], where T denotes the temperature, k B is the Boltzmann constant, and h is the dynamic viscosity of the solvent. As the concentration of Pillar-1 was increased from 2 to 50 mM, the weight-averaged diffusion coefficient (D) decreased from 6.72 Â 10 À10 to 5.62 Â 10 À10 m 2 s À1 , which was insufficient to suggest variation in the average aggregate dimensions at increasing concentrations (D 2 /D 50 ¼ 1. 19). Similarly, the azide derivative Pillar-2 showed an upeld shi for the protons of the long alkyl chain, thereby further indicating that selfinclusion of the alkyl chain took place inside the pillar [5] arene cavity.
Subsequently, azide derivative Pillar-2 containing the long alkyl chain was subjected to the CuAAC reaction at room temperature as outlined in Scheme 1. Thin-layer chromatography conrmed the consumption of the starting material, and three distinct spots were separated by column chromatography using a dichloromethane/ethyl acetate mixture for elution (80 : 20, v/v). The rst spot gave a high-resolution mass spectrometry (HRMS) signal at m/z 942.4905 [M + H] + , which is similar to the mass of Pillar-2 (i.e., m/z ¼ 941.4823 [M] + ). However, the 1 H NMR spectra of Pillar-2 and the unknown compound corresponding to the rst spot differed signicantly (Fig. S9 †) as did their melting points, i.e., 135-136 C for Pillar-2, and 209-210 C for the unknown. It should be noted here that the latter melting point corresponds to that of Pillar-3M. Thus, Scheme 1 Synthesis of a self-locked pseudo [1]catenane (Pillar-3M) and a gemini-catenane (Pillar-3D1-2) via the copper(I)-catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) reaction.
the notable upeld chemical shi observed for the methylene protons of the alkyl chain along with higher splitting pattern that reects their magnetically nonequivalent environments indicate the formation of a threaded self-locked pseudo [1]catenane, Pillar-3M, and precludes the formation of an endospirocyclic-like structure (Fig. 1a). Formation of the aromatic triazole moiety in this structure was conrmed by the downeld shi of the methine H12 proton from 2.16 to 5.65 ppm, in addition to the newly formed peaks observed at 124.5 and 140.6 ppm in the 13 C NMR spectrum of Pillar-3M (Fig. S35 †).
HRMS analysis of the isolated second (Pillar-3D1, vide infra) and third (Pillar-3D2, vide infra) spots showed similar m/z values of 1883.9242 [M + H] + and 1883.9063 [M + H] + , respectively, which represent double the mass of Pillar-3M. It was therefore suggested that these dimeric products are either nonthreaded 1 : 1 dimer or self-locked gemini-catenanes. 19 The presence of a partially reacted dimer was ruled out since the 1 H NMR data indicated the absence of a resonance corresponding to a propargyl group. Moreover, the unusual chemical upeld shi of the decamethylene protons excluded the presence of a nonthreaded dimer ( Fig. 1). Close inspection of the 1 H NMR spectra shown in Fig. 1 indicates signicant differences in the chemical shis for both the aliphatic and aromatic regions of the spectrum that was assumed to correlate to Pillar-3D1 (Fig. 1b) when compared to the spectrum of Pillar-3M (Fig. 1a), whereas the spec-trum that was considered to correlate to Pillar-3D2 (Fig. 1c) generally exhibited similar resonances to the 1 H NMR spectrum of Pillar-3M. For example, the methylene H11 protons of Pillar-3D1 split into two sets of signals with noticeably higher upeld shis (i.e., 4.74 and 4.85 ppm) compared to the same methylene signal in the Pillar-3M pseudo [1]catenane (5.49 and 5.58 ppm) and in the gemini-catenane-type structure Pillar-3D2 (5.52 and 5.59 ppm). Similarly, H8 split into two sets of signals with higher upeld shis of À1.75 and À1.65 ppm, whereas the H8 protons of Pillar-3M and Pillar-3D2 appeared as single signals at lower upeld shis of À1.02 and À1.03 ppm, respectively. These signicant Fig. 2 Different views of the X-ray single-crystal structure of the Pillar-3D1 gemini-catenane, in (a) CDCl 3 and (b) CD 2 Cl 2 . Red, grey/green/light blue, and purple represent  oxygen, carbon, and nitrogen, respectively. Hydrogen atoms are omitted for clarity.
Differences in the chemical shis observed for Pillar-3D1 suggests that the two pillar [5]arene rings are aligned in opposite directions (i.e., C2-symmetric, erythro), whereas Pillar-3D2 presents as the S2-symmetric isomer (achiral, 'thero'). All our attempts to separate the stereoisomers of Pillar-3M and Pillar-3D1 using chiral HPLC were unsuccessful at this point. The opposite alignment of the pillararene rings in Pillar-3D1 was con-rmed by single-crystal X-ray diffraction measurements following the growth of a suitable crystal from CHCl3 by the slow evaporation method (Fig. 2a). Unfortunately, all attempts to grow suitable crystals of Pillar-3M and Pillar-3D2 were unsuccessful. The melting points of the compounds isolated from these two spots were also dif-ferent, i.e., 252-253 C (second spot, Pillar-3D1) and 233-234 C (third spot, Pillar-3D2).
The unsymmetrical nature of the threaded cyclic system in Pillar-3M enables the molecular motions to be monitored by simple 1 H NMR spectroscopy. Interestingly, when the 1 H NMR spectrum was recorded at 298 K in chloroform-d immediately aer the preparation of Pillar-3M, the H12 methine hydrogen atom of the triazole moiety appeared at 6.25 ppm, whereas the H9 and H10 methylene protons of the octyl chain appeared as two sets of signals at À0.64 and À0.52 ppm and at 2.65 and 3.05 ppm, respectively (Fig. 3a). Aer allowing the NMR solution to stand for 24 h, the H9, H10, and H12 protons moved upeld, whereas the remainder of the aliphatic methylene protons shied downeld (Fig. 3b). In addition, the H11 methylene protons shied slightly downeld due to the effect of the deshielding region of the pillararene system. A similar movement of the proton signals was observed aer 48 h (Fig. 3c), while beyond 48 h, the system was in equilibrium and no further shis were detected. These results indicate that the triazole moiety moves inside the pillar [5]arene cavity and that the alkyl methylene chain moves out of the cavity due to aromatic donor-acceptor interactions between the triazole moiety and the aromatic groups in the pillararene ring. Upon drying and redissolving the sample in chloroform-d, the protons signals reset back to their original positions (Fig. 3a).
The observed molecular motion of the threaded cyclic ring in CDCl 3 was further investigated by variable-temperature (VT) 1 H NMR experiments (Fig. 4). Most notably, the chemical shi of the H12 methine proton of the triazole ring shied gradually upeld from 6.49 to 5.15 ppm when the temperature was increased from À40 to 50 C. In contrast, a downeld shi was observed for the H8 methylene protons, i.e., from À1.40 to À0.63 ppm. These results indicate that increasing the temperature led to movement of the triazole fragment inside the pillar [5]arene cavity, while at lower temperatures, the triazole ring was able to move outside of the pillararene cavity. The observed molecular motion of the threaded chain was therefore conrmed to be a reversible process induced by changes in temperature. No temperature-induced conformer inversion was observed during the VT-NMR study.
Conformational inversion studies. The chirality switching of chiral-planar pseudo [1]catenanes triggered by solvents, 18 metal cations, 21 acids, 22 and guest molecules 23 has been well documented in the literature. Such inversion occurs when one of the incorporated macrocycles in the bicyclic structure is dethreaded by aromatic ring tumbling. Thus, we screened a range of solvents (i.e., DMSO-d 6 , pyridine-d 5 , THF-d 10 , DMF-d 7 , and CD 2 Cl 2 ) for their ability to trigger the inversion of the Pillar-3M pseudo [1]catenane. The 1 H NMR spectra recorded in DMSO-d 6 , pyridine-d 5 , and THF-d 10 showed no signicant changes in chemical shis for the proton signals of Pillar-3M, indicating that dethreading did not take place (Fig. S10 †). In contrast, the proton signals of the decylene chain coalesced and shied downeld when the 1 H NMR spectra were recorded in DMF-d 7 and CD 2 Cl 2 (Fig. S11 †). For comparison, the stacked 1 H NMR spectra of Pillar-3M in CDCl 3 and CD 2 Cl 2 at 298 K are shown in    S12. † In the case of the CDCl 3 solvent system, the location of the methylene proton signals of the decylene chain in the far upeld region indicates that Pillar-3M adopted a self-included conformation (pseudo [1]catenane) as a result of the shielding effect of the pillar [5]arene cavity (Fig. S12a †). In contrast, the 1 H NMR spectrum of Pillar-3M in CD 2 Cl 2 revealed the presence of a dethreaded conformer due to peak coalescence and the downeld chemical shi of the decylene chain proton signals (Fig. S12b †). On the other hand, solvent trigger conformational inversion of gemini-catenanes from self-included "in" to the dethreading "out" conformers involve simultaneous tumbling of the functionalized aromatic units. Stacked 1 H NMR spectra for gemini-catenane, Pillar-3D1 in CDCl 3 and CD 2 Cl 2 are shown in Fig. S13. † Similarly, the threaded conformer Pillar-3D1 in CDCl 3 , show high upeld chemical shi for the methylene proton signals for the decylene chain caused by the shielding effect of pillar [5] arene cavity (Fig. S13a †), while in CD 2 Cl 2 , the decylene chain lactated outside the macrocyclic cavity as indicated by the downeld chemical shi and the coalesce of the methylene proton signals (Fig. S13b †). 1

H NMR recorder in CD 2 Cl 2 for
Pillar-3D2 shows similar behavior to Pillar-3D1 (Fig. S20 †). The conformational assignment of the de-threaded conformer of Pillar-3D1 was conrmed by single-crystal X-ray diffraction measurement when suitable crystal for analysis obtained from CH 2 Cl 2 /DMF solvent mixture (Fig. 2b).
Based on previous literature, it was considered that in CD 2 Cl 2 , the observed conformational change from self-included to dethreaded conformers was driven by host-guest complexation between the pillar [5]arene cavity and the CD 2 Cl 2 solvent. 23 To further investigate the inuence of host-guest complexation on the conformational change, adiponitrile was selected as a guest due to its ability to form highly stable 1 : 1 host-guest complexes with pillar [5]arenes, and because of its effectiveness in the conformational inversion of pseudo [1]catenane-based pillar [5]arenes. 19,23 Thus, Fig. 5 shows the 1 H NMR spectra recorded for the complexation of Pillar-3M with different concentrations of the adiponitrile guest G in CDCl 3 . Upon the addition of 1 molar equivalent of G to Pillar-3M, signicant downeld shis were observed for the methylene proton signals of the decylene chain, and the methylene proton signals of G appeared as highly shielded signals (Fig. 5c) as a result of dethreading of the alkyl chain and guest encapsulation inside the pillar [5]arene cavity. At a lower guest concentration, signals corresponding to both the self-included and dethreaded conformers were observed in the 1 H NMR spectrum (Fig. 5b).
The conformational inversion of gemini-catenanes Pillar-3D1 and Pillar-3D2 promoted by the adiponitrile guest was then studied by 1 H NMR spectroscopy. More specically, inspection of 1 H NMR spectra recorded in CDCl 3 upon the incremental addition of adiponitrile revealed that the inclusion of a single guest molecule in one pillar [5]arene cavity was sufficient to tumble the functionalized aromatic units of the gemini-catenane and trigger chiral inversion. These results are supported by the crystal structure of Pillar-3D1 obtained aer crystallization from CH 2 Cl 2 /DMF, which shows that only one pillar [5] arene cavity was occupied by a DMF molecule (Fig. S5 †). The 1 H NMR spectra of Pillar-3D1 obtained at different concentrations of adiponitrile are shown in Fig. 6. At a guest concentration of less than one equivalent, proton resonances corresponding to the self-included and dethreaded Pillar-3D1 conformers were observed in the 1 H NMR spectrum, as can be seen from the highly shielded signals of the host and guest components. Upon increasing the host-to-guest ratio to 1 : 1, no signals corresponding to the threaded dimer were observed, and the addition of two equivalents of adiponitrile consolidated the conformational change to the dethreaded conformer.
The isothermal titration calorimetry (ITC) technique has been successfully employed to study various reversible noncovalent supramolecular interactions in solution. [34][35][36] Quantitative information concerning the binding affinity and the thermodynamic parameters of the conformational change from the self-included to dethreaded conformers of Pillar-3M (10 mM) triggered by the adiponitrile guest were therefore obtained using ITC measurements. The experimentally-obtained binding molar ratio "n" of 0.91 is close to unity, which indicates a 1 : 1 host-to-guest complexation ratio, wherein the association constant, K 11 , was determined to be 4.93 AE 0.24 Â 10 3 M À1 . The negative enthalpy change (DH ¼ À11.86 kJ mol À1 ) and positive entropy change (TDS ¼ 5.26 kJ mol À1 ) for this process indicate that the guest-promoted conformational inversion of Pillar-3M was driven by both enthalpically and entropically favorable changes, and thus, it is a spontaneous process (i.e., DG ¼ À17.12 kJ mol À1 ). A similar trend was observed for the Pillar-3D1 and Pillar-3D2 gemini-catenanes, with an increased contribution of favorable entropy changes being determined (Table 1). In both cases, the experimental binding molar ratio "n" was close to 2, which indicates a 1 : 2 host-to-guest complexation ratio with association constants, K 12 , of 1.52 AE 0.08 Â10 3 (K 11 ¼ 1.43 AE 0.11 Â10 4 ) and 1.44 AE 0.14 Â 10 3 (K 11 ¼ 1.26 AE 0.09 Â10 4 ) being determined for Pillar-3D1 and Pillar-3D2, respectively. For comparison, an analogous ITC host-guest complexation experiment was carried out using the permethylated DMP5 pillar [5]arene macrocycle at 10 mM in the presence of adiponitrile as the guest. The calculated thermodynamic parameters show that the molecular binding behavior is driven by van der Waals interactions, with a major contribution also being present from a particularly favorable enthalpy change (DH ¼ À23.23 kJ mol À1 ; TDS ¼ 0.25 kJ mol À1 ). The measured association constant for the complexation of DMP5 with adiponitrile (K 11 ¼ 1.30 AE 0.07 Â 10 4 M À1 ) was found to be approximately one order of magnitude higher than Pillar-3M due to the more readily accessible macrocyclic cavity that can easily accommodate the guest molecule.

Materials and methods
Nuclear magnetic resonance (NMR) spectroscopy was carried out on a Bruker Avance II 600 MHz spectrometer (Bruker, Germany). Electron impact ionization (EI) mass spectrometry was performed using a Thermo Scientic DFS High-Resolution GC/MS mass spectrometer (Thermo Scientic, USA). Electro-spray ionization in high-resolution mode was carried out using a Waters Xevo G2-S Qtof LC MS/MS mass spectrometer (Waters, USA). Single-crystal data analysis was carried out using a Bruker X8 Prospector diffractometer (Bruker, Germany). Data were collected at À123 C (Oxford Cryosystems, UK). Flash column chromatography was performed using silica gel (Silica gel 60, 40-60 mesh ASTM, EMD Millipore, Merck KGaA, Germany). The DMF, acetonitrile, and dichloroethane solvents were distilled prior to use. All other reagents and solvents were of reagent grade purity and were used without further purication. Permethylated-pillar [5]arene (DMP5) was synthesized according to the literature. 37 Preparation of single crystals for X-ray diffraction Suitable single crystals of the synthesized inclusion complex Pillar-3D1 were grown by the slow solvent evaporation method from chloroform and a dichloromethane/DMF mixture. The data were collected on a Bruker X8 prospector diffractometer (Bruker, Germany) using Cu-Ka radiation at À123 C. The reection frames were then integrated with the Bruker SAINT Soware package using a narrow-frame algorithm. Finally, the structure was solved using the Bruker SHELXTL Soware Package and rened using SHELXL-2017/1. All non-hydrogen atoms were rened anisotropically and hydrogen atoms were rened using the riding model. CCDC 2049467 and 2081939. †

ITC measurements
All ITC studies were carried out on an Affinity ITC system (TA Instruments, USA). The data were analyzed using NanoAnalyze, Version 3.10.0. For the ITC host-guest complexation experiments, the DMP5, Pillar-3M, Pillar-3D1, and Pillar-3D2 hosts were dissolved in CHCl 3 (300 mL, 10 mM) and added to the reaction cell with CHCl 3 in the reference cell. A 100 mM adiponitrile solution was taken up into a syringe, and the titration was performed over 40 injections with a volume of 2 mL per injection and a time interval of 240 s between injections. 4-Propargyloxyphenol. Hydroquinone (6.60 g, 60 mmol) was dissolved in DMF (30 mL) at room temperature, and to this solution, potassium carbonate (12.42 g, 90 mmol) was added. This solution was stirred for 30 min and then stirred further at 80 C overnight aer the addition of propargyl bromide (4.64 mL, 60 mmol). Thereaer, the solvent was removed by evaporation under reduced pressure, and the crude product was extracted with dichloromethane. The desired compound was then puried by column chromatography using a dichloromethane/hexane mixture (75 : 25, v/v) and was obtained as a white solid following solvent evaporation (5.8 g, 65%). 1  1-(10-Bromodecyloxy)-4-propargylbenzene. 4-Propargyloxyphenol (2.20 g, 15 mmol) was dissolved in dry DMF (20 mL) at room temperature and potassium carbonate (2.80 g, 20 mmol) was added. The resulting solution was stirred for 30 min and then stirred further at 80 C overnight aer adding 1,10-dibromodecane (4.5 mL, 20 mmol). Then, the reaction mixture was poured into ice-cold water (250 mL) and the precipitate was ltered and washed with water. The desired compound was further puried by column chromatography using a dichloromethane/hexane mixture (60 : 40, v/v) and collected as a white solid aer solvent evaporation (2.9 g, 53%). 1 13  Copper(I)-catalyzed alkyne-azide 1,3-dipolar cycloaddition reaction of 1-(10-bromodecyloxy)-4-propargylpillar [5]arene Pillar-3M and Pillar-3D(1-2). 14,25 Pillar-2 (100 mg, 0.1 mmol) was dissolved in dry acetone (30 mL) and degassed for 5 min. Thereaer, a degassed solution of tetrakis(acetonitrile)copper(I) hexauorophosphate, Cu(CH 3 CN) 4   Conclusions A1/A2-difunctionalized pillar [5]arenes bearing 10-bromodecyloxy and propargyl substituents were synthesized by the co-cyclization of their corresponding hydroquinone derivatives. The 10-bromodecyloxy substituent in the pillar [5]arene rim (Pillar-1) undergoes self-inclusion, as evidenced by the unusual high upeld shi of the proton signals during nuclear magnetic resonance (NMR) spectroscopy. 1 H NMR concentration experiments and diffusion-ordered spectroscopy measurements demonstrated that the inclusion behavior of the long chain 10bromodecyloxy substituent was concentration-independent and resulted in the formation of a relatively stable pseudo [1]rotaxane. In addition, the pseudo[1]catenane and 1 : 1 gemini-catenanes were successfully synthesized by an intramolecular copper(I)catalyzed alkyne-azide 1,3-dipolar cycloaddition reaction following conversion to the A1/A2-difunctional azido-propargyl pseudo [1]rotaxane derivative (Pillar-2). In a chloroform solution, the molecular motion of the threaded ring in the unsymmetrical pseudo [1]catenane was induced by changes in the temperature, as demonstrated by variable-temperature 1 H NMR experiments. Furthermore, the conformational inversion triggered by solvents and a guest molecule was demonstrated by NMR, ITC, and X-ray analyses. The obtained data provide a molecular-level picture of the conformational inversion of the pseudo [1]catenane and gemini-catenanes triggered by an external solvent or guest stimulus. The calculated thermodynamic parameters obtained from the ITC measurements show that the conformational changes of the pseudo [1]catenane and the gemini-catenanes, i.e., from threaded to dethreaded conformers, are driven by favorable enthalpy and entropy changes. Further studies into chirality switching are currently underway in our laboratories, and the results will be presented in due course.

Conflicts of interest
There are no conicts to declare.