Biochemical Characterization of New Gemifloxacin Schiff Base (GMFX-o-phdn) Metal Complexes and Evaluation of Their Antimicrobial Activity against Some Phyto- or Human Pathogens

Four novel ligand-metal complexes were synthesized through the reaction of Fe(III), pleaseCo(II), Zn(II), and Zr(IV) with Schiff base gemifloxacin reacted with ortho-phenylenediamine (GMFX-o-phdn) to investigate their biological activities. Elemental analysis, FT-IR, 1H NMR, UV-visible, molar conductance, melting points, magnetic susceptibility, and thermal analyses have been carried out for insuring the chelation process. The antimicrobial activity was carried out against Monilinia fructicola, Aspergillus flavus, Penicillium italicum, Botrytis cinerea, Escherichia coli, Bacillus cereus, Pseudomonas fluorescens, and P. aeruginosa. The radical scavenging activity (RSA%) was in vitro evaluated using ABTS method. FT-IR spectra indicated that GMFX-o-phdn chelated with metal ions as a tetradentate through oxygen of carboxylate group and nitrogen of azomethine group. The data of infrared, 1H NMR, and molar conductivity indicate that GMFX–o-phdn reacted as neutral tetra dentate ligand (N2O2) with metal ions through the two oxygen atoms of the carboxylic group (oxygen containing negative charge) and two nitrogen atoms of azomethine group (each nitrogen containing a lone pair of electrons) (the absent of peak corresponding to ν(COOH) at 1715 cm−1, the shift of azomethine group peak from 1633 cm−1 to around 1570 cm−1, the signal at 11 ppm of COOH and the presence of the chloride ions outside the complex sphere). Thermal analyses (TG-DTG/DTA) exhibited that the decaying of the metal complexes exists in three steps with the final residue metal oxide. The obtained data from DTA curves reflect that the degradation processes were exothermic or endothermic. Results showed that some of the studied complexes exhibited promising antifungal activity against most of the tested fungal pathogens, whereas they showed higher antibacterial activity against E. coli and B. cereus and low activity against P. fluorescens and P. aeruginosa. In addition, GMFX-o-phdn and its metal complexes showed strong antioxidant effect. In particular, the parent ligand and Fe(III) complex showed greater antioxidant capacity at low tested concentrations than that of other metal complexes where their IC50 were 169.7 and 164.6 µg/mL, respectively.


Introduction
The crystal structures of several free fluoroquinolones molecules have been determined in several research [1][2][3]. It is interesting to note that in most cases the carboxylic group is not deprotonated and the hydrogen atom of this group is hydrogen bonded to an adjacent 4-oxo atom.
In few examples such as gemifloxacin, lomefloxacin, norfloxacin, moxifloxacin, and ciprofloxacin, the carboxylic group is protonated and the molecules exist in a zwitterionic form with protonated terminal nitrogen of the piperazine ring in a solid state [4][5][6]. Crystal structures of fluoroquinolones complexes indicate that neutral fluoroquinolones in the zwitterionic state are capable of forming simple complexes bidentately through one of the carboxylic oxygen and ring carbonyl oxygen [7][8][9][10].

Physico and Chemical Characterization of GMFX-o-phdn and Its Complexes
The elemental installation of the separated compounds in addition to their physical properties is shown in Table 1, which are in a decent concurrence with the proposed chemical formulae. The results acquired specified that all the isolated complexes were established by the reaction of metal ions and all the complexes reported here are hydrates with varying degrees of hydration with GMFX-o-phdn in a 1:1 molar ratio for all those metals. All complexes are stable in air, colored, non-hygroscopic powders, and soluble in DMSO and DMF. The molar conductance measurements of all the complexes in DMF solution were in the range of 173.10-272.40 Ω −1 mol −1 cm 2 [30] [typified 1:3 electrolytic for (A) complex and 1:2 for (B), (C) and (D) complexes]. The relatively high values signalize electrolytic nature of the complexes which attributed to the non-bonding of the chloride anions to the metal ions in the inner coordination sphere [17,30]. The magnetic susceptibility measurements of the solid complexes were done at room temperature and the calculated values are shown in Table 1. The magnetic moments of Fe(III) and Co(II) complexes were found at 5.81 and 5.10 B.M [31].

IR Spectra and Mode of Bonding
Careful implementing of the IR spectra of complexes and rapprochement with that of the ligand were executed to find out donation information to clarify the technique of bonding of the ligand across diverse metal ions. Thus, a deliberated implementing of the IR spectrum of the ligand was performed and the effectiveness of the metal ion binding in the vibration frequencies was analyzed. The most substantial IR spectral bands of all systems and their assignments are collected in Table 2 and their spectra are offered in Figure S1. The data educe the following observations: the GMFX-o-phdn infrared spectrum reveals the obscurity of bands due to the o-phenylenediamine group ν(NH 2 ) and ν(C=O) of gemifloxacin. Instead, newly formed very strong band at 1633 cm −1 is obtained showing the complete condensation of the amino groups with the keto group demonstrating the development of the linkage of the Schiff base [32,33]. Table 2. Selected infrared absorption frequencies (cm −1 ) for GMFX-o-phdn and its metal complexes. The IR spectra of all complexes containing hydration and coordination water molecules display bands around 3434 cm −1 due to ν(O-H) vibration mode of the water molecules which is also supported by elemental analyses [34,35]. Moreover, the appearance of bands around 840, and 600 cm −1 in the spectra of all complexes were attributed to rocking and wagging vibration of the coordinated water [36,37]. The two bands spotted at 1715 and 1633 cm −1 in the spectrum of GMFX-o-phdn were referred to the stretching vibration of carboxylic ν(COOH) and the azomethine group ν(C=N), respectively [36][37][38]. The missing of the band at 1715 cm −1 in all complexes and the shift of the distinctive band of azomethine group to a lower value from 1633 cm −1 to 1528 cm −1 specified the participation of one oxygen of the carboxylate group and C=N group in the interaction with metal ion forming six-membered rings (Scheme 1) [35]. The bonding is also reinforced by the occurrence of new bands with medium intensity appearing at 640-492 cm −1 which assigned to ν(M-O) and ν(M-N) stretching vibrations, respectively [38].

UV-Vis Absorption Spectra
The electronic spectral data are often useful in the estimation of results provided by other methods of structural examination. The assignments of the important electronic spectral bands ( Figure S2) of ligand and its metal ion complexes are shown in Table 3. Assorted bands in the electronic spectra of GMFX-o-phdn demonstrated bands at 33,898 and 31,746 cm −1 (Table 3) which may be assigned to π-π* and n-π* transitions, respectively; these transitions take place in the condition of unsaturated hydrocarbons containing groups of ketones or groups of azomethine [39,40]. The complexes exhibited new bands in the range of 22,222-20,408 cm −1 , which may be attributed to a combination of ligand-metal charge transfer (MLCT) [41]. The electronic spectrum of (A) complex shows absorption band at 17,241 cm −1 corresponding to 6 A 1 -4 T 2 (G) transitions and the spotted magnetic moment value at 5.81 B.M, suggesting the complex's high octahedral spin [38,42]. The electronic spectrum of Co(II) complex, [CoC 42 H 58 F 2 N 12 O 13 Cl 2 ] ( Figure S2), shows recognizable bands at 18,181 and 16,949 cm −1 ; the presence of these bands are consistent with those expected for six coordinate Co(II) complex [43]. These bands can be assigned respectively to 4 T 1g (F) → 4 T 1g (P) and 4 T 1g (F) → 4 T 1g (F) transitions [44,45]. Molar absorptivity (ε) obtained from the electronic spectra of complexes were recorded by using the relation: A = εcl, where A = absorbance, c = 1 × 10 −3 M, l = length of cell (1 cm) [46].

Thermal Studies (TG and DTG)
TG and DTG analyses were performed for affirmation of the molecular structure of the complexes, their thermal stabilities, and revelation of the diverse types of solvents of crystallization. The temperature ranges and weight losses together with DTG peaks are indexed in Table 5 and showed in Figure S4. All synthesized compounds are subjected to thermo gravimetric analysis within temperature range from 25 • C to 1000 • C in nitrogen. The GMFX-o-phdn was thermally decomposed in two sequential decomposition steps. The first and second steps with estimated mass loss of 98.40% (calculated mass loss = 98.65%) within the temperature ranges 32-114 • C and 114-810 • C may be attributed to the loss of 1.5H 2 O and 18C 2 H 2 + 2HF + 2NH 3 + 2C 2 N 2 + CO 2 + 2NO 2 + 2N 2 . The TG curve of complex (A) has three degradation steps, the first one has 7.87% weight loss (calculated weight loss = 7.91%) in the range 33 • C to 133 • C, indicating the removal of five hydrated water molecules. The second step with weight loss 37.20% (calc = 37.40%) at maximum temperature 203 • C supporting the removal of two coordinating H 2 O and 15C 2 H 2 molecules. The third step with two maxima at 317 • C, 435 • C with 42.80% weight loss (calc: 42.42%), corresponding to the removal of 3C 2 H 2 + 3HCl + 2HF + CO + 2NH 3 + 1.5H 2 O + 2NO + N 2 molecules and forming 0.5Fe 2 O 3 and five carbon as residue. TG thermograms of the three solid complexes (B), (C), and (D) showed three decomposition steps. The first step occurs at 67, 113, 62, and 74 • C maxima temperatures, respectively, with the mass loss of 8.10%, 9.50%, and 7.90% corresponding to the loss of lattice water. The second step occurs at 212, 326, and 172 • C maxima temperatures, respectively, with the weight loss 38.20%, 30.70%, and 23.70% corresponding to the loss of 15C 2 H 2 + 2H 2 O, 12C 2 H 2 + 2H 2 O, and 9C 2 H 2 + 2H 2 O for (B), (C), and (D) complexes. The third step with 436, 394, 366, and 448 • C maxima temperatures, respectively, with a weight loss 41.17%, 47.80%, and 53.40% giving CoO + 5C, ZnO + 4C, and ZrO2 + 4C as final products. The decomposition mechanisms are only based in speculation and thermal analysis without a complementary technique (gas chromatography). The suggested residues confirmed only on the basis of weight loss% calculation. According to the prior DTA survey, the DTA nitrogen thermogram of GMFX-o-phdn with metal complexes under study offers several phases, as shown in Figure 1. In the DTA curves, the chemical modifications that follow the removal of water, anion, and ligand molecules are spotted as exo-or endothermic peaks. At 88, 189, 282, and 439 • C, the GMFX-o-phdn offers these four peaks. The peaks 88, 282, and 439 • C are endothermic peaks with −4.77, −0.35, and −0.55 µV, respectively, except 189 • C is exothermic peak with 11.01 µV with this activation energy. The (A) complex has severalpeaks (endothermic and exothermic) of 1.20, 2.51, and −0.63 µV activation energies at different temperatures of 137, 203, and 328 • C respectively. The complex (B) manifested three peaks at 72, 119, and 329 • C for A. Evaporation of absorbed water is imputed to the first and second peaks and well-defined as endothermic peaks at 72 and 119 • C and activation energy of −3.24 µV and −0.99 µV. At 329 • C, the third peak reflects −1.39 µV of decomposition and activation energy. DTA curve of (C) complex manifests two peaks at 67 and 395 • C. The first endothermic peak with activation energy of −3.84 µV at 67 • C matched to lack of water molecules hydration and the last peak at 395 • C matched to the abstraction of coordinated water and GMFX-o-phdn molecules with activation energy of −0.53 µV. Finally, DTA curve of (D) complex presented four peaks at 80, 197, 331, and 448 • C. The first endothermic peak with activation energy of −4.36 µV at 80 • C matched to lack of water molecules hydration and the last three peaks at 197, 331, and 448 • C matched to the abstraction of coordinated water and Schiff base GMFX-o-phdn molecules with activation energies of 1.50, −1.92, and −1.49 µV.

Calculation of Activation Thermodynamic Parameters
Kinetic parameters of the decomposition steps were specified by non-isothermal approaches such as Horowitz-Metzger (HM) and Coats-Redfern (CR) methods [48,49].
Coats-Redfern equation: Horowitz-Metzger equation: The higher activation energy (Ea) values represent the thermal stability of the complexes and are summarized in Table 6. For the subsequent decomposition phases, the increase of the ∆G* value implies that the rate of elimination of the GMFX-o-phdn would be less than that of the prior GMFX-o-phdn and the increase of T∆S* from one phase to another, Figure S5. Compared to the previous complex that needs more energy, T∆S*, this can be due to the structural rigidity of the residual complex following exclusion of one or more GMFX-o-phdn for its rearrangement before undergoing any modulation. Entropy has been shown to have negative numbers in all complexes, which further proposed their stability [50]. The positive value of ∆H* imply that the decay is endothermic. Where: ( a ) correlation coefficients of Arrhenius plots; ( b ) standard deviation; (Ea) the activation energy; (∆S * ) the entropy of activation; (∆H*) the enthalpy of activation; and (∆G*) Gibbs free energy.

Antifungal Effect
The obtained results showed that the studied GMFX-o-phelin ligand exerted promising antifungal activity against all the tested pathogenic fungi except M. fructicola (Table 7). Whereas the highest activity of the parent ligand was observed in the case of P. italicum at 1000 ppm (Table 7). On the other hand, all studied metal complexes showed fungicidal effect against A. flavus and B. cinerea in a dose-dependent manner, where the highest activity was observed against A. flavus using complex (B) at 1000 ppm and against B. cinerea using cycloximide 50 µg/mL, complex (A) 1000 ppm and (B) at 1000 ppm ( Table 7). The only antifungal activity against M. fructicola was observed in the case of (B) at the three tested concentrations. Regarding P. italicum, cycloximide 50 µg/mL showed the highest significant inhibition activity followed by (A) at 1000 ppm, whereas (C) at 1000 ppm showed only moderate activity.

Antibacterial Effect
Regarding the antibacterial activity of the studied GMFX-o-phdn and its metal complexes, the obtained results showed that all tested treatments were able to inhibit the growth of tested pathogenic bacteria especially at the higher tested concentration (400 µg/mL) compared to the positive control tetracycline (50 µg/mL) as illustrated in Figure (

Mechanism of Antimicrobial Action
The obtained results of antimicrobial test showed that the tested ligands and their metal complexes were able to inhibit the growth of all studied strains in a dose-dependent manner. In particular, the fungicidal effect of the studied compounds could be due to the chemical structure of the free ligand itself as well as the toxicity of the studied metal ions [51][52][53]. The explanation for the promising biological activity of the studied complexes can very well be traced back to several reasons: (i) The concept of cell permeability and chelation process can reduce the polarity of a metal ion through the partial sharing of the positive charge with the donor groups of the ligand; (ii) the chelation process can also increase the delocalization of electrons on the whole chelate ring and enhance the lipophilic nature of the synthesized complex which facilitate its passage into the lipid membranes for penetrating the microbial cells [17,36]; (iii) the potential antimicrobial efficacy of the studied ligand and its metal complexes is thought to be highly related to the specific toxicity of the inorganic salts of metals ions. The obtained results gave a good insight for the possible use of these new prepared compounds for safely controlling different phytopathogens.
On the other hand, the antifungal and antibacterial activity of the studied gemifloxacin ligand and its metal complexes could be correlated also to their ability to inhibit both DNA gyrase and DNA topoisomerase IV enzymes [54]. Furthermore, various metal ions as cobalt, copper, nickel, and zinc were potentially used against several pathogens, where they form low molecular weight complexes and therefore penetrate more efficiently into microbial cells [54].
Recently, most of the antimicrobial drug resistance of some bacterial pathogens is a critical world issue especially for some important pathogens such as: E. coli, P. aeruginosa, Enterococcus spp., and Staphylococcus spp. [55,56]. Therefore, search for effective treatments and control of such serious microorganisms remains an important challenge for many researchers all over the world. Several research reported the antimicrobial activity of fluoroquinolones ligands against many pathogenic microorganisms, but they are less active against G-ve bacteria than ciprofloxacin [57,58]. In particular, gemifloxacin ligand demonstrated promising antimicrobial activity, compared to ciprofloxacin and levofloxacin, especially against G +ve bacteria such as: S. aureus, penicillin-susceptible and penicillin-resistant strains of Streptococcus pneumoniae [59][60][61][62]. On the other hand, some fluoroquinolones ligands such as gemifloxacin and moxifloxacin are less active against P. aeruginosa as is ciprofloxacin, hence the obtained results showed promising antibacterial effect against this pathogenic bacterial either in the case of GMFX-o-phdn or the complexes (A), (B), and (C). On the same context, the obtained results of the current research are in agreement with Morissey and Smith [63] who reported that the following three studied quinolones ligands (ofloxacin, levofloxacin, and ciprofloxacin) showed higher antibacterial activity against P. aeruginosa than other G-ve bacteria [64]. Meanwhile, the use of many fluoroquinolones such as gatifloxacin, moxifloxacin, and gemifloxacin in clinical field for controlling some pathogenic microorganisms resulted to be safe and had no cytotoxic effects. However, there is still concern about the possible emergence of pathogens resistance [65,66].

Antioxidant Activity
Results of the antioxidant activity of GMFX-o-phdn and its metal complexes showed strong antioxidant effect either at 500 or 250 µg/mL (Figure 3). In particular, the parent ligand and G.Fe complex showed greater antioxidant capacity at low tested concentrations than that of other metal complexes where the IC 50 of GMFX-o-phdn was 169.7 and 164.6 µg/mL, respectively (Table 8). Whereas the highest significant antioxidant activity was reported for the metal complexes observed in the case of (A) complex at 500 µg/mL ( Figure 3). On the other hand, (B) and (C) complexes showed the lowest antioxidant activity where the IC 50 was 300.2 and 362.2 µg/mL, respectively compared to other tested compounds ( Table 8). The RSA % of the GMFX-o-phdn metal complex could be due to their hydrogen donating ability [51,67]. In addition, the high scavenging activity of the parent ligand may explain its moderate antimicrobial effect because the reduced compounds are unable to penetrate microbial cell walls, which is characterized by its negative charge, therefore these compounds became difficult to break down and lose the microbe cytoplasm [51].

Materials and Reagents
Whole chemicals applied were of the analytical reagent grade (AR), and of highest purity available. They include gemifloxacin (GMFX), ortho-phenylenediamine (o-phdn), ferric chloride, cobalt chloride hexahydrate, zinc chloride monohydrate, zirconyl chloride heptahydrate, potassium dichromate, concentrated sulfuric acid, commercial grade concentrated nitric acid 69%, hydrogen peroxide 20%, disodium salt EDTA, ammonium hydroxide, ammonium chloride, Variamine Blue, Eriochrome Black T, gallein (Pyrogallolphthalein), and silver nitrate and were provided by Obour Pharmaceutical Industrial Company (Cairo, Egypt), Sigma Aldrich chemicals (Darmstadt, Germany), respectively. Organic solvents used were absolute ethyl alcohol and dimethylformamide (DMF). Distilled water was commonly applied in all preparations. All glassware were steeped overnight in chromic mixture (potassium dichromate + concentrated sulfuric acid) rinsed thoroughly with bidistiled water and dried in an oven at 100 • C.

Preparation of GMFX-o-phdn Schiff Base
An ethanolic solution was made by mixing GMFX (2 mmol, 0.77 g) with o-phenylenediamine (1 mmol, 0.108 g) then was once refluxed in the existence of 1 mL of glacial acetic acid for 4 h. The resulting combination was once settled on a water bath then cooled down to 0 • C. The dark red precipitate was filtered off, washed multiple times with ethanol, and dried below vacuum over CaCl 2 (Scheme 2).

Instruments
The elemental analyses were performed using a Perkin Elmer 2400 CHN elemental analyzer. The M % content was evaluated using three analytical methods like complexometric titration, thermogravimetry, and atomic absorption. For complexometric titration A digestion procedure was performed to breakdown the metal complexes. Digestion was carried out by addition of 2 mL nitric acid and 1 mL of hydrogen peroxide to the beaker containing a measured weight of metal complex. Then the beaker was placed on the hot plate temperature but not higher than 85 • C and covered with an elevated watch glass and allowed to cool and dilute to 25 mL in a volumetric flask with bi-distilled water. The percentage of the metal ions were determined gravimetrically by transforming the solid products into metal oxide, and also by using atomic absorption method [25]. Atomic absorption analysis was carried out by direct method to estimate the total metal content at the corresponding wavelength. Several reference standard solutions of each metal were prepared with a specific concentration. Spectrometer model PYE-UNICAM SP 1900 fitted with the corresponding lamp was used for this purpose. FT-IR spectra in KBr discs were recorded in the range from 4000-400 cm −1 with FT-IR 460 PLUS Spectrophotometer. 1 H NMR spectra were recorded on Varian Mercury VX-300 NMR Spectrometer using DMSO-d 6 as the solvent. TG-DTG measurements were done under N 2 atmosphere within the temperature range from room temperature to 1000 • C using TGA-50H Shimadzu, the mass of sample was accurately weighted out in an aluminum crucible. Electronic spectra were analyzed using UV-3101PC Shimadzu. The absorption spectra were recorded as solutions in DMSO-d 6 . Room temperature magnetic susceptibilities of the powdered samples were analyzed on a Sherwood scientific magnetic balance using Gouy balance at room temperature using Hg[Co(CSN) 4 ] as the calibrant. Melting points were recorded on a Buchi apparatus. All measurements were carried out at ambient temperature with freshly prepared solutions. The molar conductance of 1 × 10 −3 M solutions of the ligands and their complexes in DMF was measured at room temperature using CONSORT K410.

Antifungal Activity Assay
Tested fungi. The following tested phytopathogenic fungi, Monilinia fructicola, Aspergillus flavus, Penicillium italicum, and Botrytis cinerea, were cultured on potato dextrose agar (PDA) and stored at 4 • C as pure cultures in the mycotheca of School of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Potenza, Italy. All tested fungi were previously identified by morphological and molecular methods. Fungicidal assay. The fungicidal activity of the tested compounds was determined by incorporation assay in PDA medium [65] at concentrations 1000, 800, and 400 µg/mL. PDA Petri dishes (Ø 90 mm) were inoculated with each single fungal disks (Ø 5 mm). All plates were incubated at 22 ± 2 • C for 96 h and the antifungal effect was evaluated by measuring the diameter of the mycelium growth (mm). The growth inhibition percentage (GI%) was calculated according to Zygadlo et al. [66] (Formula 1) compared to cycloheximide 50 µL/mL.
where GI (%) is the percentage of mycelium growth inhibition, Gc is the average diameter of fungal mycelium in PDA (control), and Gt is the average diameter of fungal mycelium on the treated PDA dish.

Antibacterial Activity Assay
Tested bacteria. The tested pathogenic bacterial strains Escherichia coli, Bacillus cereus, Pseudomonas fluorescens, and P. aeruginosa were conserved as pure cultures in the collection of SAFE.
Bactericidal assay. The antibacterial activity of the tested compounds were evaluated following the disc diffusion method [65,66]. For this trial, the bacterial suspension of each tested strain was prepared in sterile Millipore H 2 O and incorporated with soft agar (0.7%) at ratio 1:9 (v/v) to reach 10 8 colony forming units (CFU/mL). Four milliliter of each suspension was poured in Petri dishes (Ø 90 mm) filled with king B nutrient media (KB). Blank discs (6 mm) (OXOID, Milan, Italy) were placed on each Petri dish and 15 µL of each tested compound was added at 400, 200, and 100 µg/mL concentrations. Tetracycline (50 µg/mL) was used as positive control. The antimicrobial effect was determined after 24 h at 37 • C by measuring the average diameter of inhibition zones (mm) ±SDs of three replicates.

Antioxidant Activity
Antiradical activity of the studied compounds was evaluated using 2,2 -azinobis (3ethylbenzthiazoline-6-acid) (ABTS •+ ) assay following the methodological procedures of the basic principles of Martysiak-Żurowska and Wenta [68]. The ABTS method is considered the most sensitive method and is characterized by higher repeatability for observing the kinetics of specific enzymes. The detectability and sensitivity of ABTS •+ are higher than the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay [51,69]. The stock solution of the ABTS radical was prepared by dissolving 38 mg of ABTS in 10 mL of an aqueous sodium persulphate solution (2.45 mM). The mixture was dark stored for 16 h. For the analysis, 1 mL of stock ABTS •+ solution was diluted in ethanol (1:30). Twenty microliter of sample was added to 980 µL of ABTS •+ solution for 2 h in dark at room temperature. After centrifuging (5 min, 8000 rpm), absorbance was measured at 734 nm against the reference solvent (ethanol). The solutions were prepared fresh for the analysis and all determinations were carried out in triplicate. Evaluation of the radical scavenging activity (RSA%) of the studied ligand and its metal complexes was carried out at 250, 500, 1000, and 2000 µg/mL concentrations (Formula (2)): RSA % = (1 − A t /A c ) × 100%; (6) where A t is the absorbance of sample and A c is the absorbance of colorimetric radical substance without sample.

Statistical Analysis
The obtained results of the antimicrobial assays were subjected to one-way ANOVA for the statistical analysis. The significance level of the outfindings was checked by applying Tukey B Post Hoc multiple comparison test with a probability of p < 0.05 using statistical Package for the Social Sciences (SPSS) version 13.0 (Prentice Hall: Chicago, IL, USA, 2004).

Conclusions
Chemical structures of a novel GMFX-o-phdn Schiff base ligand and its mononuclear metal complexes were portrayed employing diverse physicochemical techniques. The current research disclosed an octahedral geometry around the metal complexes as specified from UV-Visible spectra and magnetic moment measurements. IR spectra disclosed that the bis-Schiff base ligand acts as a tetra dentate ligand and its pattern of coordination is through the nitrogen atoms of the azomethine and oxygen of the carboxylate group. The thermal behavior was studied in order to give an idea about thermal decomposition of the complexes. Moreover, 1 H NMR spectra for complexes demonstrate a new peak at 3.45 and 3.50 ppm, due to the existence of water molecules in the complexes. On the other hand, the studied complexes exhibited promising antimicrobial activity against most of the tested phytopathogens and strong antioxidant effect.