Expression, Purification, and Comparative Inhibition of Helicobacter pylori Urease by Regio-Selectively Alkylated Benzimidazole 2-Thione Derivatives

The urease enzyme has been an important target for the discovery of effective pharmacological and agricultural products. Thirteen regio-selectively alkylated benzimidazole-2-thione derivatives have been designed to carry the essential features of urease inhibitors. The urease enzyme was isolated from Helicobacter pylori as a recombinant urease utilizing the His-tag method. The isolated enzyme was purified and characterized using chromatographic and FPLC techniques showing a maximal activity of 200 mg/mL. Additionally, the commercial Jack bean urease was purchased and included in this study for comparative and mechanistic investigations. The designed compounds were synthesized and screened for their inhibitory activity against the two ureases. Compound 2 inhibited H. pylori and Jack bean ureases with IC50 values of 0.11; and 0.26 mM; respectively. While compound 5 showed IC50 values of 0.01; and 0.29 mM; respectively. Compounds 2 and 5 were docked against Helicobacter pylori urease (PDB ID: 1E9Y; resolution: 3.00 Å) and exhibited correct binding modes with free energy (ΔG) values of −9.74 and −13.82 kcal mol−1; respectively. Further; the in silico ADMET and toxicity properties of 2 and 5 indicated their general safeties and likeness to be used as drugs. Finally, the compounds’ safety was authenticated by an in vitro cytotoxicity assay against fibroblast cells.


Introduction
Urease is a dinickel enzyme that is found in several microbes, such as bacteria and fungi, in addition to plants and invertebrates. Urease was discovered in 1975 and was the first enzyme that nickel was vital for its action [1]. The function of urease is to catalyze the urea hydrolysis process to ammonia and carbonic acid [2].
Urease is an essential factor in the pathogenesis of various dangerous microbes. The pathogenic bacterium Helicobacter pylori can survive the acidic gastric through the utilization of urease to produce large amounts of ammonia and increase the pH [3].
Streptococcus salivarius depends on urease to produce ammonia, causing dental plaque and calculus [4]. Klebsiella pneumoniae and Proteus mirabilis rely on the urease to cause pneumonia, kidney stones, and urinary tract infections [5,6]. Additionally, the roles of urease in infection-induced reactive arthritis and acute pyelonephritis were reported [7,8].
In agriculture, the ureolytic bacteria increase ammonia amounts in the soil through fast urea degradation. This increase causes plant toxicity because of the high concentrations of ammonia and carbon dioxide [9].
In the present study, we have expressed and purified the urease enzyme from H. pylori. Furthermore, the urease inhibitory effects of region selectively alkylated benzimidazole-2thione derivatives have been investigated. Experimental enzyme kinetic and molecular docking studies were utilized to understand the mechanism of urease inhibition that was exhibited by the active compounds. The cytotoxicity activities of the active urease inhibitors were investigated as well.

Rationale of the Design and Synthesis
H. pylori urease has 12 active sites containing two Ni 2+ ions each. The enzyme has a tetrahedral form consisting of four triangle shapes [31]. The active site of H. pylori urease is covered by a flap of a barrel-like shape. At the bottom of the barrel, the Ni 2+ coordination site exists. This Ni 2+ site contains both a penta-and hexacoordinate nickel, with coordinating ligands (Asp362, His274, His248, His136, and His138). The binding pocket is mostly lined with hydrophobic amino acids, such as Gly279, His221, Ala169, and Ala365. Crystal structures of the ureases show that, in addition to the conserved residues in the active site, the ureases share conserved residues that make up the mobile flap that covers the active site [32,33] (Figure 1). Hydroxamic acid derivatives have emerged as the most promising candidates from among the different classes of inhibitors of urease. It was reported that hydroxamic acids with hydrophobic groups attached to them are more potent inhibitors of urease because they can easily penetrate the hydrophobic environment surrounding the active site. The -CONHO-moiety of the hydroxamic acid is also found to be necessary for chelation and inhibition of urease [34].
In this work, a series of benzimidazole derivatives having the function group (-NHCSNH-) is considered as a chemical isostere for -CONHO-moiety of hydroxamic acids. In addition, the -NHCSNH-moiety has the same configuration as urea (the classical substrate of hydrolase). Moreover, the benzene ring of the designed benzimidazole derivatives may facilitate the binding against the hydrophobic amino acid residues in the active site. Furthermore, different alkyl substitutions were carried out to increase the hydrophobicity of the synthesized compounds, hoping to reach a more efficient hydrolase antagonist ( Figure 2).

Synthesis of Benzimidazole-2-Thione Derivatives
In the present study, benzimidaole-thione (2) was utilized to prepare different derivatives through a regioselective alkylation (Scheme 1). The obtained compounds are the S, N-bis (alkylated) analogs 3a, 3b and 3c as well as the S-alkylated analogs 4a-i and N-alkylated analog 5. The strategy for synthesizing the desired alkylated analoges was based on the possible regioselective alkylation of 2 with different alkylating agents under different conditions. The benzimidazole-2-thione 2 was prepared in a good yield using two different methods according to the literature [35,36] (Scheme 1). Then, the reaction of 2 with equivalent amounts of the alkylating agents in the presence of K 2 CO 3 in dry acetone/DMF mixture with stirring overnight gave S,N-bis(alkylated) [36], respectively, in (35-45%) yield. The structure of products was assigned based on the spectral data as well as the elemental analysis.
The 1 H-NMR spectra of compounds 3a, 3b and 3c indicated bis alkylation of 2 at the sulphur and nitrogen atoms by showing the presence of singlet signals at δ = 4.20 ppm, δ = 5.80 ppm and δ = 4.46 ppm that was assigned to SCH 2, and the presence of singlet signals at δ = 5.13 ppm, δ = 5.80 ppm and δ = 5.61 that was assigned to NCH 2, respectively. The 13 C-NMR spectra of compound 3a showed the presence of 2CH 2 signals, one of them at δ = 34.2 ppm assigned to SCH 2 and δ = 44.7 ppm assigned to NCH 2 . Similarly, compound 3c showed SCH 2 at δ = 17.8 ppm and NCH 2 at δ = 31.8 ppm beside the remaining analysis that completely fit the proposed structures.
Also, the alkylation of 2 with a variety of alkyl halides (bromoethanol, 3-chloropropanol, chloropropan-2-ol,1-bromo-undecane, 2-bromo-1,1-diethoxyethane, 1-bromobutane, epichlorohydrin, 2-chloro acetic acid, and benzylbromide) could proceed through the formation of the corresponding S-alkylated analogs 4a-i, presumably due to the higher nucleophilicity of the sulphur atom. Consequently, the salts of the thiolate anions generated by protonabstraction from the thiol groups under the basic catalyst (K 2 CO 3 , Et 3 N and KOH) were initially formed and hence behaved as an ambient nucleophile in the nucleophilic substitution reactions. Direct coupling of the alkylating agents with 2 in K 2 CO 3 in dry acetone gave the corresponding S-alkylated derivatives 4a-i in 49-71% yields.
The 1 H-NMR spectra of compounds 4a-i showed the characteristic signal of SCH 2 in the range of δ =3.11 ppm to δ =4.79 ppm besides the NH group. The 13 C-NMR spectra for these derivatives showed SCH 2 signals ranging from δ = 31.3 ppm to δ = 39.7 ppm and C-S signals from δ = 146.2 ppm to δ = 153.1 ppm.
Compound 5 (Figure 3) was synthesized by the reaction of benzimdazole-2-thione 2 with chloroethoxymethane, in water/acetone containing potassium hydroxide base medium. The reaction afforded the corresponding 1-(ethoxymethyl)-1H-benzo[d]imidazole-2(3H)thione 5 in 65% yield, and the structure of 5 was established on analytical and spectral data. The IR spectrum showed bands due to NH and C=S at 3450 cm −1 and 1458 cm −1 and the mass spectrum of 5 showed a molecular ion peak at m/z 208, which compatible with molecular formula C 10 H 13 N 2 OS, 1 H-NMR spectrum showed the characteristic signal due to SCH 2 at δ = 5.66 ppm and NH at δ = 12.55 ppm. Both singlets, as well as 13 C-NMR, showed the presence of C=S at δ = 169.6 ppm and SCH 2 at δ = 27.1 ppm that completely fits the proposed structure 5.

Expression, Purification from H. pylori
The H. pylori DNA was extracted using a DNA extraction kit (Qiagene, Hilden, Germany). The Urease C gene was amplified by PCR using the purified genomic DNA as a template. Specific primers were synthesized to amplify the intact region of the genes according to Bickley [37]. The PCR product was then qualitatively analyzed on 1% agarose gel to give an expected size of 360 bp and deposited in the gene bank under the accession number of KF233428 ( Figure 4). The PCR product was recovered using the Thermo Scientific gel extraction kit, and the amplified product was then purified and used for cloning and expression purposes.

Cloning and Transmission of Purified PCR Product
The recombinant gene of the pH6HTN His6HaloTag T7-urease C was transformed into a BL21 E. coli strain, and the fusion protein was expressed. The recombinant urease enzyme was purified from the recombinant E. coli. The obtained urease enzyme was determined using the Bradford method. It was observed that 200 mg/mL activity was determined.
Moreover, the minimum activity of the enzyme after the evaluation was 20 mg/mL with a fold of 0.40. After that, the recombinant proteins from E. coli after being concentrated were injected on FPLC lop and detected by UV at the absorbance of 280 nm and the urease activity was observed in the first peak. The purified urease enzyme was analyzed by SDS-page for the fusion protein with predicted molecular masses of 45 KD ( Figure 5).  Different bacterial isolates were obtained, characterized, and identified as H. pylori using biochemical and molecular techniques. The molecular identification revealed that the selected isolates are H. pylori with identical 95 and 99%. Urease genes were amplified using specific primers and amplicons with molecular size 360 bp (H. pylori urease C gene) were observed ( Figure 6). The obtained results were similar to those reported by Agent's [38] and not agreed with by those obtained by Hossein [39]. The purified PCR products were quantified and then cloned into pGEM-T easy cloning vector, sub-cloned using EcoR1 into, pH6HTN His6HaloTag, prokaryotic expression vector, and the result revealed that white colons after transmission were observed and transferred into L.B medium containing IPTG. The overnight cells were subjected to lysis and protein purification. The protein purification by FPLC column chromatography showed single peaks ( Figure 6). The activities enzyme purified was 200 mg/mL with fold 0.40 with the recovery of 98%, these results were similar to 190,230 mg/mL that was reported by Mohamed [40] and Pınar [41] and different to 200 mg/mL. When comparing the activity of the recombinant urease with the standard urease enzyme (J. bean), the H. pylori purified urease gene enzyme showed a similar ammonia production to the standard urease enzyme. When the fractions were separated on SDS-PAGE gel faint band at molecular weight 45 kDa ( Figure 6) with the pooled fractions from the column chromatography, very distinct bands were observed in the FPLC fraction. The results are similar to those obtained by Hossein [39] and Leila [42]. Figure 6. Gel filtration-chromatography (FPLC) of H. pylori urease C gene equilibrated with gelpermeation buffer at 0.5 mL/min, where the 4-mL volume of recommended proteins was applied to the column after adjusting the salt concentration to running buffer. Proteins were detected at 280 nm.

Urease Inhibition Assay
The alkylated benzimidazole 2-thione derivatives were screened for urease inhibition. Only two compounds, 2 and 5 exhibited interesting inhibition activities against urease enzymes. Other compounds showed weak inhibition with percentage values less than 40% at 1 mM concentrations. The structures of the active compounds are shown in Figure 3.
The results in Table 1 shows that compound 2 was found more specific to H. pylori than the J. bean urease enzyme. Compound 5 was found more specific to H. pylori urease than the J. bean urease enzyme. It was observed that compound 2 is one of the non-competitive inhibitors against H. pylori, and J. bean ureases. This compound was able to decrease the V max value without affecting the affinity of the enzyme-substrate (K m value). On the other hand, compound 5 founded to be an uncompetitive inhibitor against H. pylori urease enzymes as it decreased both the V max and K m values. Controversially, the compound appeared to be a non-competitive inhibitor of J. bean urease as it only decreased the V max value without affecting the affinity of the enzyme-substrate (K m value). Other compounds from the alkylation of the benzimidazole 2-thione group, showed weak activity against all the urease enzymes.

Molecular Docking
Depending on Figure 1, which clarified the essential amino acid residues in the catalytic active site of H pylori hydrolase, we carried out the docking studies for the most active compounds 2 and 5 against Helicobacter pylori urease (PDB ID: 1E9Y, resolution: 3.00 Å). The docking studies were carried out to investigate the possible binding interaction of the synthesized compounds against the prospective biological target (Helicobacter pylori urease).
Validation of the docking process was achieved through the running of the docking procedure for only the co-crystallized ligand ((acetohydroxamic acid, HAE) against the active pocket. It was found that the produced RMSD value between the generated pose of the docked molecule and the original one was equal to 1.63 Å. This indicates the validity of the docking process ( Figure 7). At first, the binding mode of the classical urease inhibitor (acetohydroxamic acid, HAE) was investigated as a reference molecule. Additionally, the binding role of the two Ni +2 at the active site was clarified. Then, the binding pattern of the synthesized compounds (2 and 5) was examined to compare their binding mode with that of the reference molecule (HAE).
Comprehensive docking studies were carried out using MOE14.0 software. These studies resulted in free energy (∆G) values which indicate the binding interaction of the tested molecules with the target protein, as shown in Table 2.
The binding mode of the co-crystallized ligand (acetohydroxamic acid, HAE) against Helicobacter pylori urease showed binding energy of −7.78 Kcal. Mol −1 ). It exhibited four hydrogen bonds with the essential amino acid residues in the active site. In addition, such compounds exhibited three electrostatic interactions with Ni +2 which facilitate the interaction with the receptor. The C=O group was incorporated in the hydrogen bonding interaction with His221. Additionally, The OH group formed a hydrogen bond with Asp362. Furthermore, the NH group exhibited two hydrogen bonds with Ala365 and Asp362 ( Figure 8).  The results of the docking studies revealed that compounds 2 and 5 had occupied the same pocket and showed the like binding mode of the co-crystallized ligands. Interestingly, the hydrophobic moieties of the synthesized compounds were incorporated in many hydrophobic interactions in the active and resulted in higher binding energy than the co-crystallized ligand (Figure 9). The binding mode of compound 2 against Helicobacter pylori urease showed binding energy of −13.89 kcal/mol. It exhibited three hydrogen bonds, four hydrophobic interactions, and seven electrostatic attractions. In detail, the C=S group formed three hydrogen bonds with Gly279, Asp362, inside the urease subunit KCX219. In addition, The C=S group formed four electrostatic interactions with His221, His136, His274, and His138. Moreover, the C=S moiety formed two co-ordinate interactions with the Ni +2 . Additionally, the aromatic system formed four hydrophobic interactions with Ala365, Ala169, Cys321, and Ala365. Additionally, the imidazole ring formed one electrostatic attraction with Arg338 ( Figure 10). Compound 5 showed good binding mode against Helicobacter pylori urease with a high binding energy of −17.20 kcal/mol). The NH group formed one hydrogen bond with Ala365. The C=S group formed two hydrogen bonds with Asp362 inside the urease subunit KCX219. Additionally, it formed four electrostatic interactions with His274, His136, His138, His221. Additionally, it formed two co-ordinate interactions with the two nickel atoms in the active site. The ethoxymethyl group formed three hydrophobic interactions with His322, His248, and His221. The aromatic system formed one hydrophobic interaction with Cys321, and two electrostatic interactions with Arg338 and Met366 (Figure 11).

ADMET Studies
In silico ADMET parameters were investigated for the most active candidates 2 and 5. The co-crystallized ligand (acetohydroxamic acid, HAE) was used as a reference drug. Discovery Studio 4.0 was used to predict ADMET descriptors. The predicted descriptors are listed in Table 3. The results revealed that compound 2 has a medium BBB penetration level, whereas compound 5 showed high BBB penetration power.
For aqueous solubility and intestinal absorption levels, the tested compounds exhibited a good level. Both 2 and 5 were also predicted to be a non-inhibitor of CYP2D6. The plasma protein binding revealed that compound 2 exhibited plasma protein binding power < 90%. In contrast, compound 5 showed plasma protein binding power < 90% (Figure 12).

Toxicity Studies
Toxicity prediction was carried out for compounds 2 and 5 based on the validated and constructed Discovery Studio software [43,44]. Table 4 showed that the tested compounds have low toxicity. For the carcinogenic potency TD 50 rat model, compound 2 showed a TD 50 value of 74.796 mg/kg body weight/day, less than that of the reference drug HAE (82.223 mg/kg body weight/day). On the other hand, compound 5 showed a higher TD 50 value (127.982 mg/kg body weight/day) than HAE. Regarding the rat maximum tolerated dose model, the tested compounds 2 and 5 showed a maximum tolerated dose of 0.115 and 0.072 g/kg body weight, respectively. These values were less than the reference compound (0.135 g/kg body weight).
For the rat oral LD 50 model, the tested compounds showed oral LD 50 values of 0.271 and 0.314 mg/kg body weight/day, respectively. These values were less than that of HAE (1.090 mg/kg body weight/day). For the rat chronic LOAEL model, the tested compounds showed LOAEL values of 0.069 and 0.034 g/kg body weight, respectively, which were less than HAE (0.423 g/kg body weight). The tested compounds were predicted to be an irritant against the skin and ocular irritancy models.

In Vitro Cytotoxicity
The two active urease inhibiting compounds were subjected to a cytotoxicity test. The obtained data clarified that both compounds 2 and 5b showed cellular viability IC 50 at 0.031 and 0.062 µM, respectively. The result indicated that compounds 2 and 5 are not cytotoxic in high concentrations (Table 5).  1 H NMR spectra were recorded on a Bruker Avance AV NMR spectrometer at 300 or 400 MHz, whereas the 13 C NMR was recorded on the same instrument at 75 or 100 MHz, respectively, with TMS as the internal standard. Mass spectra were recorded on a Finnigan (MAT312) and Jeol (JMS.600H) instrument; HRMS was recorded with Thermo Finnegan (MAT 95XP). Solvents used were purified by simple distillation.
Method (i): A mixture o-phenylene diamine 1 (5.4 g, 0.05 mol), and thiosemicarbazide (4.6 g, 0.05 mol) was heated in an oil bath at 180-190 • C, where the mixture was melted and then solidified after 1 h. The reaction mixture was cooled and recrystallized from ethanol to give a brown solid. Method (ii): o-Phenylene diamine 1 (17.7 g, 0.1 mol) was suspended in a mixture of ethanol 100 mL, and water 15 mL. Carbon disulfide (9 g, 0.11 mol), and potassium hydroxide (6.3 g, 0.11 mol) was added, and the mixture was heated at reflux for 6 h. A charcoal 4 gm was added and refluxed for 10 min. The hot solution was filtration and diluted with water 100 mL, and 8 mL of glacial acetic acid. The precipitate was collected by filtration, and recrystallization from ethanol the glistening white crystals was obtained.

General Procedure for the Synthesis of Compounds 4a-i
A mixture of compound 2 (0.15 g, 1.0 mmol), and triethylamine (0.14 mL, 1.0 mmol) in dry acetone (25 mL) containing DMF (2 mL). Then the appropriate alkyl halides (1.2 mmol) were added. Stirring was continued overnight and the mixture was filtered and washed with acetone. The solvent was evaporated under reduced pressure and the product was subjected to column chromatography to give titled compounds 4a-i.

General Procedure for the Synthesis of Compound 5
A mixture of compound 2 (1.5 g, 0.01 mol) in aqueous potassium hydroxide (0.6 g, 0.01 mol) in water (15 mL) was treated with the appropriate alkyl halide (0.012 mol) in dry acetone (30 mL), and stirred at room temperature until the reaction was judged complete by TLC using EtOAc-n-hexane (4:6). After completion of the reaction, the solvent was evaporated under reduced pressure and the product was subjected to column chromatography to give titled compound 5.

Urease Expression and Purification
The bacterial DNA was extracted using a DNA extraction kit (Qiagene, Hilden, Germany). The Urease C gene was amplified by PCR using specific primers to amplify the gene according to Bickley [37] (5 TGGTAGAAAACGCTTTAGTA-3 ) as the reverse primer with a restriction site of EcoR1 and (5 AAACGCCCACACCCACATCTATCA-3 ) as the forward primer with a restriction site of EcoR I. The amplified PCR product was then qualitatively analyzed on 1% agarose gel. The PCR product was recovered using the Thermo Scientific gel extraction kit (Thermo Scientific, Waltham, MA, USA), and the purified PCR product was used for cloning and expression purposes. The expected size of the target fragment was 360 bp.

Cloning and Transmission of Purified PCR Product
The purified PCR product was ligated into the pGEM-T Easy cloning vector (Promega, Madison, WI, USA) and the ligation reactions were transformed into the competent E. coli DH-5-α according to the kit procedures. Plasmid DNA was isolated from the white recombinant cells using a Miniprep plasmid extraction kit (Thermo Scientific, Waltham, MA, USA).

Insert Released of Urease C gene from the Recombinant Plasmid
The purified DNA plasmid was subjected to restriction digestion using a fast digest EcoR1 restriction enzyme, and the reaction was performed in 20 µL reaction volume with recombinant units of enzyme and appropriate buffers at 37 • C for 5 min [the digestion reaction consists of 2 µL plasmid DNA (100 ng), 2 µL of enzyme buffer, 1 µL of restriction enzyme EcoR1 (50 U) and the volume was made up to 20 µL with nuclease-free water. The digested DNA was separated on 1% agarose gel to confirm the release of the insert, and the released gene insert was eluted using a gel extraction kit (Qiagene, Hilden, Germany).

Sub-Cloning of the Urease Gene into Expression Vector
Eluted DNA insert 1 µL (3 ng) was ligated with the pH6HTN His6HaloTag T7 expression vector 1 µ (1 ng/µ) (Promega, Madison, WI, USA) after being digested by the same restriction enzyme. The ligation mixture was incubated at 4 • C overnight and the reaction was transformed into the E. coli BL21 and plated on the LB/ampicillin/IPTG/X-gal plate, according to Sambrook [45].

Recombinant Urease C Enzyme Extraction and Purification
After 24 h of incubation, the cells were harvested by centrifuge at 12,000 rpm for 10 min at 4 • C; the pelleted cells were flooded with 2 mL of phosphate buffer (buffer Q, pH 7.5). The suspended cells were collected in a centrifuge tube and the previous step was repeated several times. The suspension was sonicated on ice for 5 min, and then centrifuged at 10,000 rpm for 30 min, and the supernatant was removed with a Pasteur pipette and subjected to purification by the FPLC system.

Recombinant Urease Concentration and Specific Activity Determination
Determination of protein content in the fractions was carried out according to the Bradford [46] method with bovine serum albumin as a standard and the urease specific activities were estimated according to the Weather [47] method, while the purity of the obtained urease enzyme was performed by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) according to Sambrook [48].

Urease Assay and Inhibition on the Recombinant Urease C
Reaction mixtures comprising 25 µL of urease enzymes solution and 55 µL of sodium phosphate buffers containing 100 mM urea were incubated with 5 µL of tested compounds above (0.5 mM concentration) at 30 • C for 15 min in 96-well plates. Urease activity was determined by measuring the ammonia production using the indophenol method as described by Weatherburn [47]. Briefly, 45 µL of each phenol reagent (1% w/v phenol and 0.005% w/v sodium nitroprussside) and 70 µL of alkali reagent (0.5% w/v NaOH and 0.1% active chloride NaOCl) were added to each well. The absorbance at 630 nm was measured after 50 min using a microplate reader (SPECTRO star Nano, Ortenberg, Germany). All reactions were performed in triplicate in a final volume of 200 µL. The results (change in absorbance per min) were processed by using MARS Data Analysis Version 2.41, software (SPECTRO star Nano, Ortenberg, Germany).
The assays were performed at pH 6.8. The percentage of inhibitions were calculated from the formula 100 − (OD test well /OD control ) × 100. Thiourea was used as the standard inhibitor of Urease.
The concentrations of the test compounds that inhibited the hydrolysis of substrates by 50% (IC 50 ) were determined by monitoring the effect of various concentrations of these compounds in the assays on the inhibition values. The IC 50 values were then calculated using Graph Pad Prism 6 software.

Toxicity Studies
The in silico toxicity profiles were calculated for compounds 2 and 5 using Discovery studio 4.0 [55][56][57][58], as shown in the Supplementary File.

In Vitro Cytotoxicity Assay
The active compounds were selected and subjected to cytotoxicity evaluation using the neutral red assay. Cytotoxic assays were performed according to Borenfreund and Puerner [62].

Conclusions
In conclusion, following the general features of urease inhibitors, compounds 2 and 5 were designed and synthesized through the alkylation of benzimidazole 2-thione deriva-tives. Both in vitro inhibition kinetics against H. pylori ureases and in silico molecular docking studies have been shown by both compounds. In silico ADMET and toxicity investigations indicated the general safety and drug-likeness of both compounds. The safety of the compounds was confirmed by an in vitro cytotoxicity assay against fibroblast cells. Compounds 2 and 5 could be effective agents against several serious pathogenic bacteria such as Helicobacter pylori, Streptococcus salivarius, and Klebsiella pneumoniae as well as several bacteria that infect plants. Further in vivo and preclinical studies would help in providing further insights into the pharmacological properties of these leading compounds.