The diversity and utility of arylthiazoline and aryloxazoline siderophores: challenges of total synthesis

Siderophores are unique ferric ion chelators produced and secreted by some organisms like bacteria, fungi and plants under iron deficiency conditions. These molecules possess immense affinity and specificity for Fe3+ and other metal ions, which attracts great interest due to the numerous possibilities of application, including antibiotics delivery to resistant bacteria strains. Total synthesis of siderophores is a must since the compounds are present in natural sources at extremely small concentrations. These molecules are extremely diverse in terms of molecular structure and physical and chemical properties. This review is focused on achievements and developments in the total synthesis strategies of naturally occurring siderophores bearing arylthiazoline and aryloxazoline units.


Introduction
Iron is an exceptionally versatile cofactor that is indispensable for a plethora of biochemical reactions in both mammalian hosts and pathogenic microorganisms. Its acquisition and regulation is crucial for efficacious microbial growth and survival. Deciency can hinder vital cellular processes such as respiration and proliferation, while an excess may cause toxic effects by inducing oxidative damage through reactive oxygen species produced in the Fenton and Haber-Weiss reactions. 1 In the pre-oxygen era, microorganisms were able to exploit highly soluble Fe(II). Although iron is still abundant in the Earth's crust, in the aerobic environment Fe(III) exists as insoluble ferric hydroxide, largely inaccessible to microorganisms.
To supply and regulate iron, microorganisms have evolved sophisticated iron acquisition and trafficking systems. 2, 3 Under iron depleted conditions, bacteria and fungi produce siderophores, 4 low molecular weight (MW under 1500 Da) molecules with high binding affinity for ferric iron, capable of solubilizing the ferric hydroxide polymers and sequestering iron from a host's proteins and ligands. Low iron concentrations trigger a "signal" to start biosynthesis of the appropriate siderophores and the proteins involved in siderophore uptake machinery. As soon as sufficient amount of iron is transported and accumulated inside the cell, its acquisition system is turned off. 5 Since the early 1950s, when the rst three siderophores were isolated and identied as growth factors of bacteria, 270 different siderophores have been structurally characterized (from over 500 identied) and this number is growing. 6 The process of isolation of siderophores from natural sources is very tedious, inefficient, and thus expensive. The unique chemical structure diversity and numerous applications both in medicine and industry, prompted scientists to prepare these molecules by total synthesis (more in Sections 3.1 and 3.2). Over the last decades only two reviews concerning the synthesis of naturally occurring siderophores were published. 7,8 Total synthesis allows for obtaining analogs of naturally occurring siderophoresmimics, which are extremely important in biomedical applications, for example in the Trojan horse strategy (antibiotic therapy). This approach allows for a deeper investigation of the relationship between structure and metal complexing properties or bioactive function. In the retrosynthetic analysis essential to plan the total synthesis of siderophores, genetic and biochemical knowledge of siderophore biosynthesis is extremely useful. Siderophore retrosynthesis is based on nding the structure of target compound synthons that would correspond to the following stages of biosynthesis. 8,9 The review covers total synthesis strategies of naturally occurring siderophores with arylthiazoline and aryloxazoline subunits. The keynote of this work is to present known strategies for preparation of this particular group of siderophores with a detailed analysis of the newest and most efficient approaches. This overview allows tracking the progress in this area made over the years, and identifying the possibilities for further development. In addition, the review describes the synthesis of analogues of some siderophores with arylthiazoline and aryloxazoline subunits, thus showing the impact of structural variations on the activity of these compounds.
Main classication of naturally occurring siderophores is based on the type of functional groups involved in the chelation of iron ions includes three basic groups such as catecholate, hydroxamate, and a-hydroxycarboxylate (Fig. 1). 10 To date more than 100 siderophores bearing analogs of this type of donors have been isolated. 11 In our review, we would like to focus on siderophores that have an aromatic phenolate and/or catecholate fragment in their structure linked directly to a thiazoline/oxazoline moiety, which will be called arylthiazolines/ aryloxazolines (this nomenclature is not commonly used in siderophores classication).
Numerous reviews have been published in the literature describing the types of siderophores produced by microbes, their properties, functions, as well as their acquisition machineries; for a comprehensive discussion, the readers are referred to selected reviews and the references therein. [3][4][5][6][7][8][9][10][11][12] Arylthiazoline and aryloxazoline-type siderophores, so far have been only rarely discussed as a group, 8,13 showing the diversity of the structures and the total synthesis approach, all illustrated by most prominent examples.
2 Origins and total synthesis of arylthiazoline and aryloxazoline siderophores and analogues

Arylthiazoline siderophores: origin and total synthesis
The group of arylthiazoline-based siderophores are characterized by the presence of a 2-thiazoline framework in the structure of a substituted aromatic ring and two or three linearly connected heterocyclic subunits. Phenolic hydroxyl group, the nitrogen atom of the thiazoline ring and usually a terminal carboxyl group are involved in iron coordination. The group members differ mainly in substituents of the phenolic ring, and modications of the terminal thiazolidine fragment. Moreover, the conguration of the stereogenic centres should be also taken into account (Fig. 2).
2.1.1 Pyochelin. Pyochelin is a prototype of arylthiazolinebased siderophores. It was rst isolated in 1978 from iron-decient cultures of Pseudomonas aeruginosa ATCC 15692 by Liu and Shokrani. 14 Cox and co-workers were pioneers in studying pyochelin properties, 15,16 and established its structure in 1981. 17 Fig. 1 The iron-binding moieties in various types of siderophores. Pyochelin is produced mainly by Gram-negative Pseudomonas 18 and Burkholderia, 19 highly aggressive opportunistic pathogenic bacteria that are common causative agents for severe respiratory infections affecting patients with compromised immunity. Moreover, P. aeruginosa is responsible for highly lethal hospital-acquired infections as it is unaffected by many disinfecting agents and its strains are oen resistant to the majority of antibiotics. 20 However, pyochelin was also isolated from Gram-positive bacteria, Streptomyces. 21 This unusual siderophore is built of one phenol moiety connected to thiazoline ring which is attached to thiazolidine part with a methyl-substituted nitrogen atom and a carboxylic group attached to the adjacent position. It has three chiral centres at positions C-4 0 , C-2 00 and C-4 00 with absolute congurations (R,R,R) (pyochelin I), but the C-2 00 -centre readily isomerizes to the (S) form (pyochelin II) yielding a equimolar mixture of two epimers. 22 On the other hand, Schalk et al. 23 reported about four diastereoisomers which were obtained by organic synthesis: pyochelin I (4 0 R,2 00 R,4 00 R), pyochelin II (4 0 R,2 00 S,4 00 R), neopyochelin I (4 0 S,2 00 R,4 00 R) and neopyochelin II (4 0 S,2 00 S,4 00 R) with varying proportions depending on starting compound (Fig. 2).
The synthesis of pyochelin was rst described in 1988 by Cox and co-workers in a three-step non-stereoselective procedure. 16 The acid intermediate 1 was prepared by method of Mathur et al. 24 and converted to the corresponding aldehyde 2, which was condensed further with L-N-methylcysteine 3, providing a mixture of pyochelin isomers 4, with an overall yield of approximately 5% (Scheme 1).
In a following paper, 25 by optimising the reaction conditions, the overall yield was improved to 65%. The performed synthesis allowed to obtain four out of eight possible stereoisomers in the 4 : 1 : 1 : 4 ratio (Fig. 3). Based on the comparison of NMR spectra to the one of 4-methylpyochelin I methyl ester, whose structure was resolved by X-ray crystallography, Cox assigned the absolute conguration of four stereoisomers, i.e., pyochelins I and II and neopyochelins I and II.
An improved synthesis of pyochelin was described by Zamri and Abdallah, who proposed a stereocontrolled synthetic pathway and obtained a mixture of four diastereoisomers in the 2 : 1 : 2 : 5 ratio (Scheme 2). 26,27 In the new proposed synthesis, the method of Mathur et al. 24 for acid preparation (1, Scheme 2) was replaced by a protocol described by Bergeron et al. to avoid epimerization of the C-4 0 stereogenic centre. 28 The acid 1 was converted to N-methoxy-N-methyl hydroxamate intermediate 7; N,O-dimethylhydroxylamine was coupled to compound 1 using diethyl cyanophosphonate (DECP) as coupling reagent, providing hydroxamate 7 in excellent yield (94%); reduction of the hydroxamate 7 by excess LiAlH 4 afforded an aldehyde 2. The nal condensation step was accomplished by the known methodology between aldehyde 2 and L-N-methylcysteine hydrochloride 3 in a 4 : 1 mixture of ethanol and water in the presence of potassium acetate, and yielded 70% of pyochelin 4 as a mixture of four diastereoisomers. In natural pyochelin, the absolute congurations are (4 0 R) and (4 00 R). During the synthesis of pyochelin, partial epimerization occurs at C-4 0 while the absolute conguration at C-4 00 remains unaffected thus providing a mixture of pyochelin isomers: pyochelin I and II, and neopyochelin I and II.
Synthetic analogs of pyochelin can enrich the library of biologically active molecules, to tune the chemical or physical properties of the pyochelin, as well as to provide a tool for the investigation of the structure-function relationship of the transporters. An appropriate architecture of ligands and the geometry of complexes allow a control of efficient iron coordination and bacterial recognition. However, the epimerization at the C-2 00 chiral centre shows the difficulty in preparing enantiopure synthetic pyochelin analogs.
The rst pyochelin analogs were only accessed by mutasynthesis (Fig. 4). 29 The mutasynthetic pyochelin analogs as mixtures of two rapidly interconverting isomers I (4 0 R,2 00 R,4 00 R) and II (4 0 R,2 00 S,4 00 R) were compared to pyochelin in their ability to transport iron in P. aeruginosa. Isomeric forms were transformed into methyl esters and separated (in case of 8c only one form I was obtained). 4-Methylpyochelins 8b and epi-8b were more active than pyochelin in 55 Fe(III) transport, while the other two analogs 5-uoropyochelin 8a and epi-8a and 6-azapyochelin 8c demonstrated a decreased iron uptake.
Extensive work in the eld of synthetic pyochelin analogs was performed by the group of Schalk, Mislin and their coworkers. The Schalk group focused on studying the properties of several biologically active analogs and conjugates of pyochelin 9a-c, as will be described on several examples. In the rst generation, the effect of the conguration on bacterial uptake was tested (Fig. 7). 30 Uptake rates of 55 Fe(III), tested on P. aeruginosa (ATCC 15692) as well on its pyoverdine defective mutant CDC5 (pPVR2), suggested that the conguration at carbon C-4 0 has no effect on the biological properties of pyochelin. Zinc complexation of the mixture (4 0 R,2 00 R,4 00 R) and (4 0 R,2 00 S,4 00 R) pyochelins provided exclusively (2 00 R) diastereomer, which indicated template direction effect. Moreover, substitution in the aromatic part (R 1 , Fig. 7) or amine group in thiazolidine part (R 2 , Fig. 5) did not affect dramatically the biological properties of the corresponding analogs compared to pyochelin. In an additional series of pyochelin analogs, the thiazoline ring was replaced by a thiazole moiety (Fig. 6). 31 The series was prepared using the synthetic protocol for pyochelin analogs through Weinreb amide intermediate, 32 and tested for 55 Fe uptake by P. aeruginosa, strains PAO1 and PAD07. As phenol function is crucial for the coordination of Fe(III), 33 analogs bearing a methylated hydroxyl group failed to transport Fe(III), and only analogs 10a, 10c, 10e and 10f were able to transfer iron inside the bacteria.
2.1.2 Yersiniabactin, micacocidin and piscibactin. Micacocidin belongs to a well-known family of zinc-containing metallophores. 34 It was isolated from Pseudomonas culture primarily as Zn(II) complex together with Cu(II) and Fe(III) analogs, and extensively studied by the Aburahi Laboratories. 35 The micacocidin structure was determined by single crystal Xray crystallographic analysis of its Zn(II) complex, 36 which indicated the presence of an aliphatic side chain on the aromatic ring and a methyl substituent in the nitrogen atom in the thiazolidine ring. This metabolite is also produced by the economically relevant plant infecting bacterium Ralstonia solanacearum (which produces staphyloferrin B as main siderophore), 37,38 and shows strong activity against Mycoplasma pneumoniae, a pathogen responsible for community-acquired pneumonia. 34 Total synthesis of micacocidin was performed by Ino and coworkers. [39][40][41] There are ve stereogenic carbon atoms in its structure. Cysteine was utilised as a chiral source for C-9, C-12 and C-18 centres, and for generation of the (S) secondary alcohol on C-14 by a stereoselective reduction of ketone. C-10 chirality was generated at a nal stage by formation of a Zn complex.
Arylthiazoline part was synthesised starting from 3-methoxy-N,N-dimethylbenzylamine 11 which was converted to intermediate 12 in 5 steps and a total yield of 70%, using the procedure described by Kamikawa. 42 Next, condensation of 12 with pmethoxybenzyl (PMB)-protected D-cysteine provided compound 13 which was cyclized using PCl 5 to obtain thiazoline derivative in 95% yield. Methyl deprotection of both ether and ester using boron tribromide provided carboxylic acid 14, which was subsequently converted to Weinreb amide 15 using bis(2-oxo-3oxazolidinyl)phosphinic chloride (BOP-Cl) coupling reagent. Following phenol protection by tert-butyldiphenylsilyl (TBDPS) group, the Weinreb amide was reduced by LiAlH 4 to afford aldehyde 16, the desired segment of micacocidin (Scheme 3).
The second synthon 41 was prepared starting from thiazolidine 18 which could be obtained from L-cysteine hydrochloride 17 in a two-step route, 43 followed by the reaction with carbonyldiimidazole (CDI) and a subsequent condensation with methyl isobutyrate, leading to keto-ester 19. Reduction of the ketone function and construction of an oxazolidinone ring provided compound 20. Condensation of 20 with 2-methyl-(S)cysteine methyl ester hydrochloride using CDI yielded 21. Reaction with TFA in reuxing toluene resulted in cyclization of To understand structure-antymicoplasma activity relationship of micacocidin, Ino and co-workers prepared several derivatives by modifying hydroxyl and carboxylic groups in the skeleton of the siderophore isolated from the fermentation of Pseudomonas sp. No. 57-250 (Fig. 8). 44 MIC values were determined for three strains: Mycoplasma gallisepticum, M. pneumoniae, M. hyopneumoniae. Compounds 27 and 30g were active, while a bulky ester group PCB 30a, amide derivatives 30b-d as well as methyl or MOM derivatives at the phenolic position 30fh, 30j-k resulted in a reduced activity. According to the authors, the lack of bulky groups and the presence of hydrogen bonds are required for activity, and the active derivatives have to possess a similar spatial conformation. The relationship between conguration and activity was examined as well.
Regardless of the C-9 conguration, C-10 (R) isomers presented a similar activity to micacocidin 29, while C-10 (S) isomers exhibited a reduced activity. These results supported the authors' proposal that the potency of siderophores depends on the ability of the derivatives to adopt a folded conformation.
In an additional work, 45 micacocidin analogs 31a-f were generated by exploiting precursor-directed biosynthesis approach. The series varied in alkyl side chain, thus did not induce signicant changes in the spatial folding. Almost all gallium complexes showed promising activities against M. pneumoniae ATCC 29342, and comparable to the native micacocidin. Derivative 31b was an exception, and exhibited a signicantly reduced activity. A minimum alkyl chain length might be important for the interaction with the biological target ( Fig. 9).
Yersiniabactin was rst isolated from Yersinia bacterial strain, a causative agent of a broad range of diseases such as plague, bowel infections and reactive arthritis. 46 Aerwards it was found that this strong chelating agent is not limited to one particular bacteria species and can be utilized by different kinds of microbes such as Enterobacteriaceae (E. coli and S. enterica). [47][48][49] The yersiniabactin system is the most frequently carried, genetically non-conserved siderophore system in uropathogenic E. coli. 50 The siderophore contains a phenol and a thiazolidine ring, as well as two thiazoline rings. Its stereochemistry is striking for the existence of ve chiral centres, with a mixture of two C-10 epimers being isolated during structure elucidation studies. 37,38 Following the preparation of micacocidin, Ino and coworkers accomplished a total synthesis of yersiniabactin ( Fig. 10). 51 The rst building block was prepared starting from condensation of Weinreb amide 32 (ref. 52) and 2-methoxybenzoyl chloride 33, which provided ester 34. Next, in a 5-step route thioamide 36 was obtained which was converted to 7 by the reaction with the Burgess reagent. Further hydroxyl group protection and reduction of Weinreb amide 7 yielded aldehyde 37 (Scheme 6).
The second synthon for yersiniabactin was prepared according to the reaction conditions used in the total synthesis of micacocidin (Scheme 4). 41 Compound 23 which was an intermediate in the synthesis of micacocidin was fully deprotected to provide derivative 38, and condensation with the rst building block 37 afforded thiazolidine 39. Ester 39 hydrolysis provided the desired yersiniabactin siderophore 40 (Scheme 7).
Piscibactin, a siderophore isolated from subspecies Piscidia of Photobacterium damselae, is known as a causative agent of sh pseudotuberculosis, a malady that causes large economic losses in marine environment. 53 High mortality rate and wide geographical distribution makes it a major problem in the mediterranean shing industry. Synthesis of piscibactin is also considered as a virulence factor in other bacteria, such as Yersinia enterocolitica responsible for severe enteric inammation in humans. 53 Piscibactin isolation was rst reported in 2012 together with an additional metabolite, prepiscibactin, presumably a possible intermediate of piscibactin biosynthesis. 53 The piscibactin structure was determined by Souto et al. 53 and the studies performed allowed to establish the stereochemistry of piscibactin as (9R,10S,12R,13S).
A successful approach for piscibactin synthesis (close analogue of yersiniabactin) was accomplished by Jiminez et al. in 2021. 54 Before, Segade and co-workers proposed a total synthesis of prepiscibactin. 55,56 Retrosynthetic analysis of piscibactin was based on three synthons, and in all three cysteine was utilized as starting material (Fig. 11). Piscibactin was prepared from two key synthons, and D-and L-cysteine were used as chiral sources for C-9 (S) and C-12 (S), respectively (Fig. 11). Applying a similar methodology as for pyochelin preparation, 26 the rst synthon was obtained through the formation of Weinreb amide, and reduction of the resulting hydroxamic ester with LiAlH 4 (Scheme 2).
The second synthon, thiazoline based amino thiol was prepared utilizing multi-functionalized carboxylic acid 41 and (S)-a-azido-methylcysteine. 57 Synthetic route for full protected carboxylic acid 41 was accomplished through protection of Lcysteine 6 and a subsequent transformation in a stereoselective manner leading to nal building block formation 41 in 20% of overall yield (8 steps). For preparation of thiazoline fragment 46 it was necessary to couple the activated acid of the protected statine 39 with a freshly prepared thiol 42 using EDCl. In the Staudinger reduction and a subsequent intramolecular aza-Wittig reaction, thioester 41 was transformed into the thiazoline 44 in good yield. Further deprotection of amino thiol 44 led to the second building block 46 in 42% yield. Condensation of thiazolinic aldehyde 2 with thiazoline 46 in dichloromethane afforded an epimeric mixture of methyl esters of piscibactin which were hydrolyzed and submitted without purication to complexation with gallium using Ga(acac) 3 . Final HPLC separation gave piscibactin Ga 3+ complex 47 and its 9S epimer in ratio 2 : 1 and 10% yield (Scheme 8).
2.1.3 Anguibactin. Anguibactin was rst isolated in 1986 from marine bacterial strain Vibrio anguillarum 775 by Actis et al. 58 It is a common sh pathogen responsible for vibriosis, a deadly haemorrhagic septicaemic disease affecting various marine and freshwater sh. The disease has high morbidity and mortality rates and causes severe economical loses in the sh industry. 59 This siderophore was of particular interest for scientists for one more reason. Its structure resembles very closely another iron carrier compound, namely the oxazoline- derived acinetobactin (vide infra) utilized by multidrug-resistant Acinetobacter baumannii, an opportunistic pathogen responsible for many lethal hospital-acquired infections. 60 Acinetobactin was found to be unstable and readily converted to its isoxazolidinone form while anguibactin was proven to be resistant to isomerization.
The structure of anguibactin can be described as being built of two planar sections, one containing the phenyl-thiazole system with its substituents and another with the imidazole ring. It was rst elucidated in 1986, 58 and the initial suggestion was later conrmed by van der Helm et al. solving an X-ray structure in 1989. 61 The total synthesis of angiubactin was carried out using a convergent approach. 60 It was initiated by preparation of the phenylthiazole core in a similar synthetic pathway as for pyochelin, 26 by condensation of L-cysteine and aryl nitrile 5a (Scheme 2). The imidazole fragment 49a was prepared in two ways. In 2010 Takeuchi et al. 62 proposed its synthesis through histamine dihydrochloride 48 transformations into 4-(2-chloroethyl)imidazole hydrochloride 49 using SOCl 2 and a subsequent coupling reaction with N-tert-butoxycarbonyl (Boc)-Obenzyloxyamine 50, 63 followed by deprotection, 64 yielding Obenzyloxyhistamine 51a in 66% total yield (Scheme 9, part A). In the second approach proposed in 2015 by Kim et al., 65  a terminal olen 52a was rst synthesized from N-Boc-O-benzyloxyamine 50 and 4-bromobut-1-ene. Dihydroxylation under Ujohn conditions afforded diol 53 which was transformed into a-hydroxyketone 54 through the selective oxidation of the secondary hydroxyl group with NaBrO 3 /NaHSO 3 . 66 In the nal step, the Cu(II)-promoted imidazole formation with ammonia and formaldehyde was performed followed by deprotection with TFA which resulted in desired histamine N-oxide derivative 51a in 40% overall yield (Scheme 9, part B).
A stereoselective coupling of the intermediate 57 to a free amine of N,O-imidazole 58 mediated by HATU in dichloromethane provided product 59 (er ¼ 98 : 2). The use of tetrabutylammonium iodide (TBAI) and boron trichloride (BCl 3 ) led to a clean removal of both benzyl and methyl groups and successfully generated compound 61. Along with 61, its 3-deoxy derivative 62 was also prepared in an analogous manner (Scheme 10).
2.1.4 Ulbactin. Ulbactin is a natural siderophore which was isolated in two epimeric forms (ulbactin F and G, in the ratio 17 : 1, respectively) from marine bacterial genus of Brevibacillus 67 extracted from an unidentied sponge found near Iwate, Japan. Although Brevibacillus sp. is not considered to be a dangerous microbe, the structures of identied compounds are captivating because its core is also shared by Pseudomonas aeruginosa siderophore pyochelin, though a peculiar tricyclic ring system is present.
The compounds possess an unusual heterocyclic structure in which two thiazolidine rings are fused to form a tricyclic ring system. Aer successful isolation and chromatographic purication, ulbactin F was obtained as pale yellow crystals and its structure was elucidated by Igarashi et al. 67 A series of studies on the structure of ulbactin F allowed its identication and determination of the absolute conguration as (4 0 R,3 00 R,6 00 R,9 00 R, and 9 00 R). Ulbactin G with the same molecular composition is the epimeric form of ulbactin F with absolute conguration (4 0 R,3 00 S,6 00 R,9 00 R, and 9 00 R). 68 A peculiar feature of ulbactin F and G, namely the 6,9-imino-1H,3H,5H-thiazolo [4,3-c] [1,4]thiazepin-5-one tricyclic ring system containing two sulfur atoms and two nitrogen atoms makes it very intriguing. This ring system, but lacking N-methyl group, was also reported for ulbactin D. 67 Scheme 9 Two synthetic routes for the formation of the imidazole part 51a: (A) from histamine hydrochloride, 62 (B) from N-Boc-O-benzyloxyamine and 4-bromobut-1-ene. 65 Scheme 10 Total synthesis of angiubactin 61 and its analog 62. 60 As ulbactin F is the major metabolite (vide supra), this diastereomer was targeted in the total synthesis. 68 Convergent synthetic pathways were applied, with several building blocks prepared. Synthesis of the phenylthiazolidine core followed the pathway used for pyochelin, converting salicylnitril to aldehyde 2. 26 The thiazolidine synthons were prepared from protected enantiomeric cysteines. Reaction of the two fragments in ethanol/water with NaOAc, and then heating in an ethyl acetate/ methanol mixture, provided the product 69 in total yield of 12% and its epimer, epi-69, as a side product (Scheme 11).

Aryloxazoline siderophores: origin and total synthesis
Oxazolines are a large family of heterocyclic imino ethers possessing a ve-membered ring structure. Within the oxazoline family, 2-oxazolines have been most extensively studied so far and found broad applications in various elds. This is an important structural motif and pharmacophore of natural products and bioactive compounds (including siderophores, Fig. 12). 69 The aryloxazoline/oxazole moiety is present in many siderophores and plays a crucial role in coordination mode and iron transport machinery in microorganisms.
2.2.1 (À)-Yanglinmycin, spoxazomicins and madurastatins. In 2013, Zhang's group isolated (À)-yanglingmycin, 2aryl-substituted 4,5-dihydrooxazole derivative from the fermentation broth of Streptomyces djakartensis. 70 Though it was not clearly stated in the original paper, this compound is an (S) enantiomer of spoxazomicin C. Due to the presence of various functionalities and its biological activities, yanglingmycin has been considered as a potential component of pharmaceutical drugs, polymeric materials, insecticides and so on. 71,72 To investigate the bactericidal activities of yanglingmycin, the Zhang group 70 decided to synthesize this molecule and its analogs. In order to achieve the intended goal, an efficient synthetic pathway for the preparation of 2-aryl/ heteroaryloxazolines from nitriles under metal-and catalystfree condition was used. 73 Benzonitrile and its substituted analogs 5a-c were applied as the starting materials. In the initial step, a Pinner reaction was performed to afford the corresponding methyl benzimidate hydrochlorides 70a-c, 74 which were then reacted with various amino acid methyl ester hydrochlorides 71-73. 75 The nal products were obtained by an efficient reduction of esters into alcohols using LiAlH 4 , yielding (À)-yanglingmycin 74a and its analogs 74b-c, ent-74a-c, 75, ent-75, 76 and ent-76 in total yields of 62% and 52-65%, respectively (Scheme 12). 76 For a deeper exploration of the antibacterial potency, a series of hydroxyl ester rac-77a-z and hydroxyl ether rac-78a-o derivatives of the racemic (AE)-yanglingmycin and its phenyl substituted analogs rac-74a-h were prepared (Schemes 13 and 14). 77 To obtain 2-aryl-substituted 4,5-dihydrooxazole analogs rac-74a-h, arylnitriles 5a-h were reacted with serinol in the presence of sodium carbonate (Scheme 13). Hydroxyl ester derivatives of (AE)-yanglingmycin rac-77a-z were synthesized through esterication reactions using a catalytic amount of EDC, and hydroxyl ether derivatives 74a-o were obtained by etherications of the alcoholic hydroxyl group in the presence of a base. Additionally, a uorinated analog rac-77 was afforded via (AE)-yanglingmycin rac-74a treatment with DAST (68% yield) (Scheme 14). In vitro antibacterial studies against four Gramnegative and three Gram-positive bacteria strains revealed that some derivatives are more active than (AE)-yanglingmycin rac-74a. Structure-activity relationship analysis clearly showed that antimicrobial activity was lost aer most derivatizations of alcoholic hydroxyl group and in the absence of phenolic hydroxyl substituent. On the other hand, incorporation of a short-chain ester group, an electron-decient ether moiety and uorination of alcoholic hydroxyl group were found bene-cial for the antibacterial potency.
In 2019 Sun et al. proposed a mild catalytic approach to 2oxazolines via oxetane ring-opening reaction (a model substrate was synthetized by acylation of commercially available 3-amino oxetane). 78 This synthetic protocol is a useful tool for preparation of a diverse family of natural products with 2-oxazoline unit including spoxazomycin C rac-74a and its analogs, such as spoxazomycin D 82 and madurastatin B1 81 in racemic form (Scheme 15). It is worth emphasizing that 2-aryl-substituted 4,5dihydrooxazole derivatives are crucial building blocks in total syntheses of many natural products such as mycobactin type siderophores etc.
2.2.2 Acinetobactin, pseudomonine and msbactin. Acinetobactin is a siderophore which contains catechol-oxazoline moiety, hydroxamate unit and imidazole ring. It was rst described as the siderophore of highly resistant human pathogen A. baumannii, 79 and later on to be also produced by the sh pathogen Aeromonas salmonicida subsp. salmonicida 80,81 (a Gram-negative g-proteobacteria identied as the causative agent of furunculosis, a devastating disease affecting cultured and wild sh worldwide). The structure of acinetobactin was initially proposed, 79 but further research revealed facile and spontaneous rearrangement from oxazolinyl hydroxamate into isooxazolidinone form. 82,83 This phenomenon is similar to the previously observed conversion of prepseudomonine to pseudomonine, another siderophore produced by Pseudomonas uorescens with salicylate-oxazoline moiety in the structure (Scheme 16). 84 The isooxazolidinone of pseudomonine is generated through the rearrangement of a preceeding oxazoline hydroxamate condensation product (prepseudomonine). Walsh et al. revealed that the mode of heterocyclization for N-O-C Scheme 12 The synthetic route to yanglingmycin enantiomers and its analogs. 76 Scheme 13 Synthesis of (AE)-yanglingmycin and its phenyl-substituted analogs. 77 bond formation follows the intramolecular S N 2 mechanism and the oxygen of a hydroxamate intermediate attacks the b-carbon of the Thr-derived oxazoline leading to isooxazolidinone formation. This S N 2 rearrangement reaction involves an inversion of conguration at the b-carbon of Thr side chain. 82 Moreover, subsequent studies showed the dependence of rearrangement from oxazoline-containing form to isooxazolidinone isomer on pH and temperature. 85,86 Both isomeric forms are physiological siderophores relevant for bacteria in processes of binding and cellular delivery of Fe(III).
Apart from acinetobactin, strains of human pathogenic A. baumannii may produce two other siderophores: baumannoferrin 87 (a hydroxamate siderophore) and msbactin. 88 Production of acinetobactin and baumannoferrin is highly conserved among clinical isolates while msbactin production appears to be less common. Fimsbactin is structurally related to acinetobactin because of the presence of the catechol and hydroxamate moieties together with an oxazoline ring; it possesses additionally oxazoline and 1,3-or 1,4-diaminopropane fragments. 88 Preacinetobactin 86a and prepseudomonine 86f consist of three signicant structural subunits, responsible for metal chelation, namely catechol/phenol oxazoline, hydroxamate and imidazole fragments. The proposed synthetic routes for the formation of these siderophores are based on two condensations of key heterocyclic motifs, i.e. aryloxazoline and imidazole part. The imidazole fragment 51a was prepared in the same manner as described for anguibactin (Scheme 9). The synthesis of the second building block, aryloxazoline moiety, was initiated with preparation of O-protected benzoic acid 83ab precursors. 86,89 Crucial steps for the synthesis of phenol oxazoline moiety include amide formation via coupling of L-threonine methyl ester with protected acid 83a-b using various coupling reagents (EDC/HOBt, TBTU) and Ishihara's dehydrative cyclization using Mo(VI) oxide catalyst 90 (49-70% total yield). Takeuchi et al. proposed a different route to aryloxazoline part applying methyl imidate of 2,3-dihydroxybenzoic acid and L-threonine derivative (15% total yield). 62 In order to identify the key structural motifs of preacinetobactin 86a and prepseudomonine 86f that inuence their Fe(III) binding and cellular delivery, various analogs were synthesized. 86 Novel analogs were designed based on the modication of the iron-binding parts, i.e. aromatic, hydroxamate and imidazole fragments (Scheme 17). Proposed ironbinding mode for preacinetobactin revealed that catechol dihydroxyl and imidazole groups play crucial roles in iron binding. What is more interesting, phenolic oxazoline derivatives including prepseudomonine indicate that the 3 0 -hydroxyl group in the aromatic part is not crucial for metal binding. Further modication shows that N-methylation of the imidazole ring in both N 1 and N 3 positions did not decrease the ironbinding tendency and N 1 position is a promising drug conjugation site because modications at this site did not interfere with the iron delivery function. Additionally, all hydroxamate bridge modications abolished iron delivery in the cellular uptake machinery. Wencewicz et al. 91 prepared a rigid preacinetobactin analog via oxidation of oxazoline moiety to oxazole in order to block siderophore utilization in the pathogen growth process (Scheme 18). The strategy including rigid siderophore analogs preparation can be crucial in the synthesis of new antivirulence agents.
The stereoselective preparation of msbactins was proposed by Kim's group. 92,93 Their strategy for the synthesis was based on retrosynthetic analysis in which the framework construction was accomplished via amide bond formation in condensation reaction of two building blocks, aryloxazoline carboxylate and polyamine derivatives followed by catecholate part addition through ester formation (Fig. 13).
The preparation of the oxazoline carboxylates started from the coupling of o-xylyl-protected 3,4-dihydroxyl benzoic acid 83a 90 with L-Ser-OMe, L-Thr-OMe or D-Thr-OMe in the presence of EDC and HOBt reagents. The obtained precursors 91, 84a and ent-84a were transformed into oxazoline forms via Ishihara's dehydrative cyclization catalyzed by a Mo(VI) oxide catalyst 94 followed by mild saponication using potassium trimethylsilanolate 95 which afforded the desired products 92, 85a and ent-85a in good yields (Scheme 19, part A). The synthesis of functionalized polyamine building blocks was accomplished by amide bond formation between putrescine 92 or N-monoacetyl- possessing the spermidine moiety are represented by compounds like parabactin, agrobactin, norspermidine based -uvibactin, vibriobactin and vulnibactin, and 1,3-diaminopropane basedserratiochelin A.
For the rst time parabactin was isolated in 1975 by Tait from Paracoccus denitricans. 97 In the rst approach the structure of parabactin was not well dened, because of the unstable nature of oxazoline ring. Several years later, Neilands and coworkers 98,99 demonstrated that not an N-(2-hydroxybenzoyl)-Lthreonyl fragment (parabactin A) but rather a (2hydroxyphenyl)-4-carboxyl-5-methyl-2-oxazoline moiety is connected to the central nitrogen atom of the spermidine backbone in the original siderophore. Under the acidic conditions of Tait's isolation, the oxazoline ring was opened to the threonyl derivative (acid labile oxazoline ring). Moreover, Neilands' group showed that the hydrogen atoms of the oxazoline ring were trans to each other (Scheme 21). 99 Agrobactin was isolated from iron-decient cultures of Agrobacterium tumefaciens, the organism known to induce crown-gall in higher plants. A. tumefaciens not only produces a cancer-causing plasmid but is also interesting as a vector for incorporating foreign DNA into plants. 100 Exposure of agrobactin to acid opened the oxazoline ring giving agrobactin A, in which the UV spectra undergo a characteristic redshi, same like in the case of parabactin. 99 Fluvibactin was isolated and puried from Vibrio uvialis in 1993. It contains only one catechol-oxazoline unit and is structurally related to agrobactin from which it differs only in its triamine-backbone. 101 A stereochemically modied version of uvibactin efficiently removed iron without increasing microbial growth. 102 Vibriobactin (produced by Vibrio cholerae) is structurally highly similar to agrobactin with three minor alterations: (i) the polyamine backbone is norspermidine, (ii) it contains two Lthreonines per molecule, forming two oxazoline rings, and (iii) it contains three DHB moieties as aryl caps. The discovery of the second oxazole ring in vibriobactin suggested some interesting possibilities in its metal-binding capacity. 103 Although V. cholerae can use heme directly as an iron source, it has been shown that vibriobactin is also an important virulence factor. 104 Vulnibactin, which was isolated from culture supernatants of Vibrio vulnicus M-2799 grown in a low-iron medium, is possessing two salicylate caps that are tethered to the oxazoline ring. 105 Virulent Vibrio vulnicus is a halophilic estuarine bacterium that causes fatal septicemia and necrotizing wound infections in humans with high serum iron levels and vulnibactin enables it to acquire iron from highly iron saturated host proteins. 106 Vulnibactin is structurally related to vibriobactin, from which it differs only in its salicyloyl moieties on two oxazoline rings, while uvibactin contains only one oxazoline ring consisting of L-threonine and 2,3-dihydroxybenzoic acid attached to the central nitrogen of norspermidine. 105 Serratiochelin A was isolated from Serratia sp. V4 and discovered in S. marcescens in 1974. 107 Furthermore, in 2020 serratiochelin A was isolated from an iron-dependent co-culture of Shewanella sp. and the mechanism of its degradation to Scheme 21 The acid labile oxazoline ring. 98,99 Scheme 22 Synthesis of parabactin 106 and agrobactin 107 proposed by Bergeron and co-workers. 110,115 serratiochelin C was proposed. 108 Serratiochelins are biscatecholate siderophores that could be tetra-or hexadentate, moreover, serratiochelin A and B contain a propane-1,3diamine backbone. 109 So far total synthesis of six aryloxazoline iron chelators based on a polyamine backbone were described, namely the trisamines parabactin, agrobactin, uvibactin, vibriobactin and vulnibactin, and the diamineserratiochelin A. The pioneering works in polyamine-based siderophores synthesis and explorations were performed by Bergeron's group. They proposed total syntheses of parabactin, agrobactin, uvibactin, vibriobactin and vulnibactin. At the turn of years, this research eld was supplemented by other scientists.
Based on performed research connected with structure exploration, in 1982 Bergeron's group synthesized parabactin 106 employing a stereospecic procedure under acid free conditions to preserve the oxazoline ring. 110 A proposed synthesis employed N 1 ,N 8 -bis(2,3-dimethoxybenzoyl) spermidine 100 as a starting material, a unique reagent for the generation of spermidine phenolamides. 111 In the rst step, N-Cbz-L-threonine 101 was condensed with N 1 ,N 8 -bis(2,3dimethoxybenzoyl)spermidine 100 in the presence of coupling reagents such as NHS and DCC under basic conditions. Aerwards, both carbobenzoxy and methyl protecting groups were removed from threonylamide 102. In the last step, the most critical one, coupling of 2-hydroxybenzimido ethyl ester 104, obtained before in a Pinner reaction 112 with the deprotected threonyl amide 103 was involved. As a result of this synthetic route, parabactin 106 was obtained in 18% total yield (Scheme 22).
Another synthetic route to parabactin 106 was proposed by Fujita in 1984. 113 The researchers decided to attach the catechol moieties in the nal step of the synthesis ("inside-out" approach) in contrast to Bergeron's pathway. 110 The key intermediates for parabactin formation were dihydro-1,3-oxazole derivative (obtained in 7 steps, 40% total yield) and N 1 ,N 10bis(benzyloxycarbonyl)-spermidine. 114 In the nal step, condensation of both intermediates and deprotection of terminal amine groups of the spermidine backbone followed by incorporation of the catechol fragment led to parabactin 106 in 5% total yield.
A new concept of norspermidine based synthesis of oxazoline-containing siderophores was proposed by Ishihara and co-workers. 90 The basis of this methodology lies in the oxazoline core construction at an early stage in total synthesis. Based on previously investigated approach for oxazoline and thiazoline rings formation via Mo(VI) oxides, 94,121 preparation of uvibactin 120 and vibriobactin 124 was reported. In the synthesis, three building blocks were used: norspermidine 117, 2,3-dialkoxybenzoate 116, and 2-(o,m-dialkoxyphenol)oxazoline 115 prepared from N-(o,m-dialkoxybenzoyl)-L-threonine epi-82a via the Mo(VI) oxide catalyzed method. Dehydrative cyclization of N-(o,m-dialkoxybenzoyl)-L-threonine epi-84a was performed using MoO 2 (TMHD) 2 (Scheme 24). Aerwards, a selective amide formation was conducted using Sb(III) alkoxide-catalyzed esteramide transformation 122 of primary amine groups in norspermidine 117. The amidation of the secondary amine group in products 118 and 122 was achieved using the respective carboxylic acid 115 and EDC, HOAt coupling reagents in the presence of base. In the course of this transformation uvibactin 120 and vibriobactin 124 were obtained in overall yields of 65% and 50%, respectively (Scheme 25). This improved methodology eliminates the most inefficient step in the Bergeron methodology involving 2,3-dihydroxybenzimidate formation for oxazoline scaffold construction.
The most recent synthesis of uvibactin 120 and vibriobactin 124 was described in 2013 by Raymond's group. 101 In their approach, 2-mercaptothiazoline was used to prepare a building block necessary to install the catechol-amide units in all siderophores. The 1,3-thiazolodine-2-thione functionality reacted with the primary amine group in norspermidine 117 in a very selective way. The central amine group in norspermidine 117 due to low reactivity 90 was functionalized using HATU as a coupling reagent followed by benzyloxycarbonyl-N-Cbz-Lthreonine treatment. In the nal step, a catechol-oxazoline building block obtained via Bergeron's methodology 110,115 was used leading to the desired siderophores in total yields of 20% (compound 120) and 15% (compound 124).
In the group of synthesized polyamine siderophores, equipped with aryloxazoline moiety serratiochelin A 129 Scheme 25 Total synthesis of fluvibactin 120 and vibriobactin 124. 90 containing the 1,3-diaminopropane chain is also found. The synthetic route reported by Ehlert et al. for serratiochelin A 129 formation 107 was based on Bergeron's procedure described for agrobactin 105. 115 In the initial stage, the catechol-amide fragment of 126 was formed through N-(3-aminopropyl)benzylamide 123 125 coupling with 2,3-dimethoxybenzoic acid 109. Subsequently, benzyl protection from the terminal amine group was removed, followed by N-(L-N-t-butoxycarbonylthreonyloxy) succinimide 127 incorporation. At the end all protecting groups were removed and the oxazoline core was formed using ethyl imidate of 2,3-dihydroxybenzoic acid 105 in total yield 10% (8 steps) (Scheme 26).
Many research groups have been interested in the synthesis of analogs of naturally occurring polyamine-based siderophores. The main goal for this trend is to investigate the relationship between structure and iron transporting ability. 124 Moreover, there is an increased interest in developing new antibiotic treatments by linking antibiotics to siderophore moieties (Trojan horse strategy). Many efforts were put into the structural modications of spermidine-containing catecholtype siderophore, parabactin 106 and its natural analog agrobactin 107. 124,125 All modications of these siderophores had to be performed in such a way as to preserve the ability for iron binding and microbial growth under conditions of limited iron access. The aryloxazoline part directly linked to the central amino group in the polyamine chain was mainly modied (Fig. 14). The performed research showed that the original structure of polyamine siderophore is still favoured as compared to analogs in coordination and iron transport system study.
In the previously cited work of Bergeron et al. 118 on the synthesis of L-uvibactin 120, the synthesis of unnatural enantiomer D-uvibactin ent-120 and L-homouvibactin 145 were also presented (Scheme 27). This research revealed that modi-cations of the polyamine chain of the uviabctin 120 (L-homouvibactin 145) do not affect bacterial growth and iron uptake machinery of P. denitricans. However, an invertion of the conguration of two carbon atoms in the oxazoline ring (D-uvibactin, ent-120) inhibits microbial growth. D-Fluvibactin ent-120 still functions as deferration agent with iron clearing efficiency similar to L-uvibactin 120, but due to the stereochemistry of the created ferric complex it cannot be utilized by the microorganisms.
2.2.4 Mycobactin-type siderophores. Mycobacterium tuberculosis, a species responsible for tuberculosis, serves as a source Scheme 27 Synthesis of L-fulvibactin 120 and its unnatural analogs. 118 of various lipopeptidic siderophores called mycobactins. 126 Also strains of genus Nocardia produce similar lipid-soluble intracellular iron-binding compounds (nocobactins, formylobactins, brasilibactins, amamistatins and others). 127 Their common features include the presence of two amide and one ester connections, a seven-membered caprolactam ring at one end, and 2-aryloxazole or 2-aryloxazoline at the opposite end (Fig. 15).
The mycobactins are now quite well-known siderophores since mycobactin P was rst isolated from Mycobacterium phlei in 1949. 128 Due to the position of the hydrophobic alkyl tail (R 5 or R 6 ), mycobactins are classied into two types: P-type (aer mycobactin P) or M-type (aer mycobactin M), respectively. 129 Mycobactin J is one of the P-type mycobactins and is produced by M. avium ssp. paratuberculosis which is the causative agent of Johne's disease in cattle. 130 The carboxymycobactins are structurally related to the mycobactins except that their R 5 or R 6 alkyl side chain (Fig. 15) is shorter and terminates in a carboxylic acid, resulting in much greater hydrophilicity. 131 Pathogenic mycobacteria strains like M. tuberculosis and M. bovis express carboxymycobactins as the sole extracellular siderophores. 132,133 Nocobactins NA1 and NA2 were isolated from Nocardia asteroides grown under conditions of iron deciency. They are related in their structure to the mycobactins what was described by Ratledge and Snow in 1974. 134 Amamistatin B (isolated from the actinomycete Nocardia -Nocardia asteroides 135 ) is also structurally related to mycobactins siderophore as it contains lysine-derived N-formyl hydroxylamine. Amamistatin A has anti-proliferative, but not cell-killing, effects against several kinds of human tumor cell lines. 135 Amamistatin's anti-cancer activity is probably due to histone deacetylase enzymes inhibition mediated by the N-formyl hydroxylamine ligand. 136 Brasilibactin A, a siderophore found in Nocardia brasiliensis 137 contains oxazoline moiety and a pentadecyl substituent. Brasilibactin A is a membrane-bound siderophore with structural similarity to the mycobactin class of siderophore in mycobacteria and possesses a nearly identical molecular nucleus, which includes a hydroxamic acid, an N-hydroxyformamide, and a 2-(2-hydroxyphenyl)-D 2 -1,3-oxazoline. 138 Nocardichelins A and B were isolated from the Nocardia sp. Acta 3026 and the structural characterization of the compounds was performed by mass spectrometry and NMR spectroscopy. The chemical structures of these siderophores are closely related to mycobactin siderophores. In contrast, comparison of nocardichelins A and B to brasilibactin A reveals that only the 4,5-dihydro-2-(2-hydroxyphenyl)-4-oxazolecarboxylic acid moiety is identical. 139 First preparations of mycobactin type siderophores were described by Miller and coworkers. 142,143 They prepared mycobactin S2, an analog of mycobactin S with a long alkyl chain of the mycobactic acid residue replaced by a methyl group. 142 However, the modication resulted in lowered lipid solubility which was found essential for the siderophore activity in mycobacteria. 143 A total synthesis of mycobactin S was thus designed using a similar strategy: coupling of cobactin T and mycobactic acid derivative, i.e. two compounds formed in the saponication process of mycobactins. 143 The rst component was prepared from two building blocks, one 148 containing 2aryloxazoline residue which was constructed from methyl salicylate 146 and L-serine benzyl ester, using Burgess' reagent to induce cyclization (Scheme 28). It was connected with L-lysine derivative 150 containing hydroxamic acid moiety and a pentadecane chain (Schemes 29 and 30). Cobactin T component was also based on the blocked L-lysine as a staring material, converted to the corresponding hydroxylamine 151 which underwent cyclization to yield a seven-membered ring (caprolactam derivative) with a preserved conguration of a stereogenic centre 152. Coupling with (R)-3-hydroxybutanoic acid and deprotection gave cobactin T (153, Scheme 30). Its reaction with mycobactic acid followed by treatment with TFA afforded mycobactin S 155 (27% yield of the last stages). As it could be seen, L-amino acids and 3-hydroxybutanoic acid were used as sources of chirality.
Eleven years later, Miller's group accomplished the synthesis of amamistatin B, one of siderophores isolated form a strain of Nocardia. 127 Using methods previously developed in Miller's laboratory, 136 they prepared 2-aryl-oxazole and D-lysine-derived hydroxamic acid; a third component, (S)-3-hydroxy-2,2dimethyldecanoic acid was also obtained following the procedure introduced by Yokokawa et al. (vide infra). 144 The last building block, a cyclic hydroxamic acid 157, was synthesized by a modied route from Cbz-L-lysine 149 in 19% overall yield (Scheme 31); its conguration was preserved in the caprolactam derivative formed in the cyclization step. This component was then reacted with hydroxydecanoic acid 156, and the product esteried using a linear hydroxamate 160. Two enantiomers of the latter were used (one is shown in Scheme 31), which allowed preparation (aer deprotection and reaction with oxazole part 163) of amamistatin B 164 and its epimer. A similar protocol was applied in the synthesis of an analog lacking two hydroxyl groups, however, only the original siderophore was (moderately) active against M. tuberculosis.
A similar synthetic approach was used earlier by Yokokawa, Shioiri, and co-workers who prepared amamistatin A, which differs only by the presence of a methoxy substituent from B form. 144 In the paper describing the synthesis, a stereoselective was constructed by a stepwise addition of subsequent building blocks to the caprolactam derivative 152 (Scheme 33; this compound 152 and a linear hydroxamate 173 were prepared by previously described methods 143 ), and the overall yield of the seven steps was ca. 7.5% (part of the unreacted intermediates, however, could be recovered).
Among other compounds from the mycobactin family, a total synthesis of J form 191 was described by Kapur and coworkers in 2018. 145 The specic component of this compound, Scheme 36 Assembling of four components into mycobactin J 191. 145 Scheme 37 Synthesis of synthons used for preparation of carboxymycobactin T. 146 a long-chain carboxylic acid 178 with a double C]C bond with (Z) conguration, was prepared in 48% overall yield via oxidation of 1-tetradecanol 176 to tetradecanal, its Corey-Fuchs conversion to alkyne, its esterication with chloroformate, and hydrolysis (Scheme 34). Thus obtained acid 177 with a carboncarbon triple bond was stereospecically hydrogenated over Lindlar catalyst to give (Z)-pentadec-2-enoic acid 178. It was attached to a mycobactic acid fragment which in turn was constructed from salicylic acid 179, L-threonine methyl ester, and a fragment derived from Cbz-L-lysine 184; oxazoline 181 formation was achieved by molybdate-mediated cyclization with a retention of conguration (Schemes 35 and 36). The second synthon also required L-lysine which was converted to a cyclic amide using HATU; coupling with (2S,3R)-3-hydroxy-2methylpentanoic acid 189 (a source of two stereogenic centres in the nal molecule) resulted in the cobactin fragment 190. However, in contrast to Miller's preparations, 142,143 the attempts of esterication to yield mycobactin J were unsuccessful. Scheme 38 Solid-phase synthesis of carboxymycobactin T 203. 146 Scheme 39 Preparation of two diastereomers of hydroxyacid-caparolactam part of brasilibactin A 208. 147 Instead, a chiral pentanoic acid derivative 189 was rst connected to mycobactic acid 188, and a cyclic hydroxamic acid 186 was added in a subsequent step yielding the desired nal product 191 (21% yield over three steps).
Carboxymycobactin T 203 and its three analogs were prepared by Slomczynska and co-workers using a solid-phase approach. 146  caprolactam derivative 197 (Scheme 37) to which subsequent fragments were connected (Scheme 38): (S)-3-hydroxybutyric acid 198, another L-norleucine-derived component 200, and 2-(2-hydroxyphenyl)oxazoline-4-carboxylic acid 181 which preparation was described by Miller's group. 143 To the obtained skeleton, a monomethyl ester of heptane-1,7-dioic acid 202 was attached as a side chain and the carboxymycobactin T 203 was removed from the resin. Its epimer with the opposite conguration of carbon atom bearing methyl group was prepared by the same route with (R)-3-hydroxybutyric acid ent-198 as one of components; in case of two analogs bearing amide instead of ester linkage, separation of diastereomeric mixture appeared necessary due to unexpected epimerization.
In 2007, two research groups reported on a synthesis of brasilibactin A. In a paper of Mitchell and Shaw, preparation of two diastereomers differing in the conguration of the bhydroxyacid component allowed the assignment of absolute conguration of the naturally occurring form. 147 This fragment was prepared using the titanium-mediated aldol reaction of chiral thiazolidinethione auxiliary 204 with hexadecanal which led to syn products 205 and dia-205 with the absolute conguration dependent on the amount of (À)-sparteine added (45% yield and diasteromeric ratio >95 : 5 were noted in both cases, Scheme 39). Coupling with caprolactam-based amine 186 released the auxiliary and resulted in the respective diastereomeric products 206 and dia-206. Their subsequent reactions with the known building blocks derived from D-lysine 173 and, nally, oxazoline fragment 181 prepared from D-serine followed the previously reported procedures and led to brasilibactin A 208 (found to be a 17R,18S isomer; Scheme 40) and its (17S,18R)-analog. Independently, Ying and Hong prepared four diastereomers differing by congurations of these two stereocentres. 138 The four hydroxyacid components 213, ent-213, dia-213 and dia 0 -213 were prepared by diastereoselective aldol reactions from N-propanoyloxazilidinones (syn isomers) and Opropanoylnorephedrines (anti selectivity, Scheme 41). The Scheme 42 Preparation of three components 219, 224 and 229 of nocoardimicin B. 148 overall synthetic strategy was similar to the one of Mitchell and Shaw, and the stereochemical assignment of natural brasilibactin A 208 was identical.
Banks and Moody prepared nocardimicin B 233 from four usual components, though they modied the synthesis of particular building blocks. 148 The 2-aryloxazole part 219 was obtained by rhodium-catalyzed reaction of 2-benzoyloxybenzonitrile 216 with dimethyl diazomalonate 217; removal of the methoxy group and hydrolysis gave the 2-aryloxazole-4carboxylic acid 219 in ca. 67% yield (Scheme 42). The linear hydroxamic acid component 231 was prepared by a previously established method, while for the b-hydroxycarboxylic acid, an anti-selective aldol reaction was applied between a lactatederived (R)-ketone and dodecanal under Patterson conditions. 149 Standard four-step manipulation provided the desired hydroxyacid 224 in 25% overall yield. The cyclic hydroxamic acid 229 was prepared using ring-closing metathesis (RCM) as a key step. O-Benzyl-N-Boc-hydroxylamine 50 was converted into N-allyl derivative 226 which in turn reacted with (R)-Bocallylglycine 227 to give the respective amide 228. RCM employing Grubbs' catalyst yielded a seven-membered caprolactam derivative with a double bond 229 (59% yield from hydroxylamine derivative, Scheme 42). Starting from this building block 229, all fragments were connected subsequently, and the nal step involved a hydrogenative deprotection, but also reduction of the C]C bond, and yielded the expected siderophore 233 (Scheme 43).
Concluding this part, sidorophores containing an oxazole or oxazoline ring exhibit a signicant similarity of four main chiral synthons which can be appropriately assembled. Part of these fragments inherit chirality of the starting amino acid residues, however, others are prepared in stereoselective reactions (for example, 3-hydroxycarboxylic acid in aldol reaction). Though a total synthesis of all known members of the family has not been accomplished yet (as exemplied by other mycobactins, nocobactin, and formobactin), the general procedure for their preparation should not differ much from their analogs obtained in the laboratories.

Conclusions
In this comprehensive review we presented the total synthesis of naturally occurring siderophores bearing arylthiazoline and aryloxazoline subunits. During the years a development of synthetic strategies has been observed to nd the optimal approach for synthons used for many siderophores. The progress of characterization techniques allowed to determine congurations of all stereogenic centres and to correctly describe all stereoisomers. The yield of the total synthesis and enantiomeric purity of prepared compounds have been greatly improved which is important from the application point of view. Amounts of siderophores required in biomedical studies are typically in the range of hundred milligrams, two orders of magnitude more than typically isolated from natural sources.

Conflicts of interest
There are no conicts to declare.