Passivator-Free Microwave–Hydrothermal Synthesis of High Quantum Yield Carbon Dots for All-Carbon Fluorescent Nanocomposite Films

Based on the self-passivation function of chitosan, an efficient, and green synthesis strategy was applied to prepare chitosan carbon dots (CDs). The quantum yield of carbon dots reached 35% under the conditions of hydrothermal temperature of 200 °C, hydrothermal time of 5 h, and chitosan concentration of 2%. Moreover, the obtained carbon dots had high selectivity and sensitivity to Fe3+. Based on the Schiff base reaction between the aldehyde groups of dialdehyde cellulose nanofibrils (DNF) and the amino groups of CDs, a chemically cross-linked, novel, fluorescent composite film, with high transparency and high strength, was created using one-pot processing. Knowing that the fluorescence effect of the composite film on Fe3+ had a linear relationship in the concentration range of 0–100 μM, a fluorescent probe can be developed for quantitative analysis and detection of Fe3+. Owing to their excellent fluorescent and mechanical properties, the fluorescent nanocomposite films have potential applications in the fields of Fe3+ detection, fluorescent labeling, and biosensing.


Introduction
Quantum dots (QDs), mainly II-VI semiconductor nanoparticles, have attracted extensive attention due to their unique optical properties [1], such as fluorescence tunability [2], bioimaging [3], photodynamic therapy [4] and photocatalysis [5]. However, their application in some fields, particularly biomedicine, is hindered by their their metal content, and the environmental impact of deposited QDs is also a concern. In contrast, carbon dots (CDs) are fluorescent carbon nanomaterials with a diameter of less than 10 nm, and are mainly composed of carbon nanoparticles [6,7]. CDs not only have the same excellent fluorescence performance as traditional metal-based quantum dots, but also have good biocompatibility and non-toxicity of carbon materials. Their remarkable properties of fluorescence tunability, water solubility, optical and physicochemical stability, give them potential for applications in chemical sensing [8,9], drug delivery [10,11], photocatalysis [12], bioimaging [13] and biosensing [14], and they are expected to replace traditional semiconductor quantum dots. Similar to semiconductor quantum dots, CDs have many electrons and holes on the surface, which endow the CDs with electron acceptor and electron donor roles to act as oxidizing or reducing agents [15,16]. In addition, benefiting from the numerous hydroxyl, amino or carboxyl groups on the surface, CDs can construct luminescent materials by coordinating or non-covalent bonding with high molecular polymers or inorganic compounds [17][18][19]. Relevant studies have shown that chitosan can be used as a passivator to improve the fluorescence performance of carbon dots [20]. Based on the self-passivating function of chitosan, CDs can be prepared by using chitosan as a carbon source without an additional passivating agent or further modification.
in Figure 1. According to Lindh's [35] method, the content of DNF aldehyde group was determined to be 5.326 mmol/g, corresponding to an oxidation degree of 42.6%.

Chitosan Carbon Dots (CDs) Synthesis
A total of 2 g chitosan was fully dissolved in 100 mL 1% (w/w) acetic acid solution, and the mixture was sealed in a high-temperature and high-pressure reactor under argon protection. The high-pressure reactor was placed in a microwave synthesis reactor for 5h, with microwave power of 500 W and reaction temperature of 220 °C. A large amount of deionized water was then added to the reacted mixture and centrifuged at 10,000 rpm for 30 min to separate the yellow-brown product. The supernatant was successively filtered through 0.45 μm and 0.2 μm filters to remove impurities, and the remaining filtrate was chitosan carbon dots solution ( Figure 2). The quantum yield (Q) of chitosan carbon dots was calculated using the method of reference [36]. Quinine sulfate dissolved in 0.1 M H2SO4 solution (Qs = 0.54) was used as the standard, and the fluorescence emission spectrum and ultraviolet absorption spectrum of the reference substance and CDs were scanned. The relative quantum yield of CDs can be calculated with the following equation: (1) where Q is the quantum yield, F represents the peak area of the emission spectrum when the excitation wavelength Ex = 335 nm, and A is the absorbance of the sample at the excited wavelength of 335 nm. The subscripted "s" and "x" refer to the reference substance and the test samples, respectively.

Construction of All-Carbon Fluorescent Nanocomposite Films
A measured quantity of CDs and DNF suspensions (solid content 1.12%) were mixed and stirred in an ultrasonic reactor at 50 °C for 60 min. The mixture was suction filtered through a 0.1 μm filter membrane into a film with a thickness of 0.2 mm. The formed film was dried in a constant temperature and humidity oven at 50 °C for 24 h to obtain an allcarbon fluorescent composite film. The schematic diagram of the preparation process is shown in Figure 3. For the control experiments, fluorescent nanocomposite films with different CD contents, but the same amount of DNF, were prepared and designated as F-n, where n indicates the percentage of mCDs/mDNF.

Chitosan Carbon Dots (CDs) Synthesis
A total of 2 g chitosan was fully dissolved in 100 mL 1% (w/w) acetic acid solution, and the mixture was sealed in a high-temperature and high-pressure reactor under argon protection. The high-pressure reactor was placed in a microwave synthesis reactor for 5 h, with microwave power of 500 W and reaction temperature of 220 • C. A large amount of deionized water was then added to the reacted mixture and centrifuged at 10,000 rpm for 30 min to separate the yellow-brown product. The supernatant was successively filtered through 0.45 µm and 0.2 µm filters to remove impurities, and the remaining filtrate was chitosan carbon dots solution ( Figure 2). The quantum yield (Q) of chitosan carbon dots was calculated using the method of reference [36]. Quinine sulfate dissolved in 0.1 M H 2 SO 4 solution (Q s = 0.54) was used as the standard, and the fluorescence emission spectrum and ultraviolet absorption spectrum of the reference substance and CDs were scanned. The relative quantum yield of CDs can be calculated with the following equation: where Q is the quantum yield, F represents the peak area of the emission spectrum when the excitation wavelength Ex = 335 nm, and A is the absorbance of the sample at the excited wavelength of 335 nm. The subscripted "s" and "x" refer to the reference substance and the test samples, respectively. in Figure 1. According to Lindh's [35] method, the content of DNF aldehyde group was determined to be 5.326 mmol/g, corresponding to an oxidation degree of 42.6%.

Chitosan Carbon Dots (CDs) Synthesis
A total of 2 g chitosan was fully dissolved in 100 mL 1% (w/w) acetic acid solution, and the mixture was sealed in a high-temperature and high-pressure reactor under argon protection. The high-pressure reactor was placed in a microwave synthesis reactor for 5h, with microwave power of 500 W and reaction temperature of 220 °C. A large amount of deionized water was then added to the reacted mixture and centrifuged at 10,000 rpm for 30 min to separate the yellow-brown product. The supernatant was successively filtered through 0.45 μm and 0.2 μm filters to remove impurities, and the remaining filtrate was chitosan carbon dots solution ( Figure 2). The quantum yield (Q) of chitosan carbon dots was calculated using the method of reference [36]. Quinine sulfate dissolved in 0.1 M H2SO4 solution (Qs = 0.54) was used as the standard, and the fluorescence emission spectrum and ultraviolet absorption spectrum of the reference substance and CDs were scanned. The relative quantum yield of CDs can be calculated with the following equation: (1) where Q is the quantum yield, F represents the peak area of the emission spectrum when the excitation wavelength Ex = 335 nm, and A is the absorbance of the sample at the excited wavelength of 335 nm. The subscripted "s" and "x" refer to the reference substance and the test samples, respectively.

Construction of All-Carbon Fluorescent Nanocomposite Films
A measured quantity of CDs and DNF suspensions (solid content 1.12%) were mixed and stirred in an ultrasonic reactor at 50 °C for 60 min. The mixture was suction filtered through a 0.1 μm filter membrane into a film with a thickness of 0.2 mm. The formed film was dried in a constant temperature and humidity oven at 50 °C for 24 h to obtain an allcarbon fluorescent composite film. The schematic diagram of the preparation process is shown in Figure 3. For the control experiments, fluorescent nanocomposite films with different CD contents, but the same amount of DNF, were prepared and designated as F-n, where n indicates the percentage of mCDs/mDNF.

Construction of All-Carbon Fluorescent Nanocomposite Films
A measured quantity of CDs and DNF suspensions (solid content 1.12%) were mixed and stirred in an ultrasonic reactor at 50 • C for 60 min. The mixture was suction filtered through a 0.1 µm filter membrane into a film with a thickness of 0.2 mm. The formed film was dried in a constant temperature and humidity oven at 50 • C for 24 h to obtain an all-carbon fluorescent composite film. The schematic diagram of the preparation process is shown in Figure 3. For the control experiments, fluorescent nanocomposite films with different CD contents, but the same amount of DNF, were prepared and designated as F-n, where n indicates the percentage of m CDs /m DNF .

Characterization
The absorption spectra and transmittance of CDs solution and composite films were recorded on a UV-3200 UV-vis spectrophotometer (Mapada Instruments Co., Shanghai, China). The scanning wavelength range was 200-600 nm and the slit width was set at 2 nm. The optimal excitation wavelength and optimal emission wavelength of CDs solution were obtained by using an F-7000 fluorescence spectrometer (Hitachi, Ltd., Tokyo, Japan) with a scanning rate of 240 nm/min and a scanning frequency of 40 Hz at slits of 5/5 nm. The fluorescence properties of the composite film were characterized by setting excitation wavelength to 335 nm and emission wavelength range of 350-650 nm. An appropriate amount of FeCl3•7H2O was dissolved in deionized water to prepare Fe 3+ solutions with different concentrations, and the responsiveness of CDs and composite membranes to iron ions was investigated by the change in fluorescence intensity after adding Fe 3+ solution.
After diluting DNF and CDs solution, the microstructure of DNF and CDs was observed on a Tecnai G2-F20 Transmission Electron Microscope (FEI Co., Hillsboro, OR, USA) and a NanoScope IIIa MultiMode Atomic Force Microscope (Veeco Co., Plainview, NY, USA). After freeze-drying, the surface and cross section morphologies of the composite film were characterized using a Nova Nano SEM 230 (FEI Co.) and AFM.
The surface functional groups of the fluorescent composite films were characterized using a Nicolet 380 Fourier Transform Infrared spectroscopy (FTIR) (Thermo electron Instruments Co., Ltd., Waltham, MA, USA), in the form of KBr pellets with a resolution of 4 cm −1 . Thermal stability was analyzed in a thermogravimetric analyzer (TGA) (NE-TZSCH STA 449 F3 Jupiter ® , Selb, Germany), heating from 30 °C to 700 °C at a rate of 10 °C/min in a N2 atmosphere of 30 mL/min. The zeta potential of DNF and CDs was measured using an SZP-06 Zeta potential analyzer (Mütek, Filderstadt, Germany), and the positive and negative charges of DNF and CDs were analyzed by detecting the zeta potential.

Fluorescence Properties of CDs
As shown in Figure 4, the diluted CDs solution is transparent and clear pale yellow under natural light, and shows an excellent fluorescence effect, with strong blue fluorescence under UV excitation. The CDs have a strong UV-vis absorption feature centered at 292 nm, assigned to the n→π* transition of the surface moieties [37,38]. The phenomenon of emission spectra changing with the excitation wavelength (Figure 5a,b) is similar to that reported by Sun et al. [39]. Surface passivation may generate defect sites on the surface of

Characterization
The absorption spectra and transmittance of CDs solution and composite films were recorded on a UV-3200 UV-vis spectrophotometer (Mapada Instruments Co., Shanghai, China). The scanning wavelength range was 200-600 nm and the slit width was set at 2 nm. The optimal excitation wavelength and optimal emission wavelength of CDs solution were obtained by using an F-7000 fluorescence spectrometer (Hitachi, Ltd., Tokyo, Japan) with a scanning rate of 240 nm/min and a scanning frequency of 40 Hz at slits of 5/5 nm. The fluorescence properties of the composite film were characterized by setting excitation wavelength to 335 nm and emission wavelength range of 350-650 nm. An appropriate amount of FeCl 3 ·7H 2 O was dissolved in deionized water to prepare Fe 3+ solutions with different concentrations, and the responsiveness of CDs and composite membranes to iron ions was investigated by the change in fluorescence intensity after adding Fe 3+ solution.
After diluting DNF and CDs solution, the microstructure of DNF and CDs was observed on a Tecnai G2-F20 Transmission Electron Microscope (FEI Co., Hillsboro, OR, USA) and a NanoScope IIIa MultiMode Atomic Force Microscope (Veeco Co., Plainview, NY, USA). After freeze-drying, the surface and cross section morphologies of the composite film were characterized using a Nova Nano SEM 230 (FEI Co.) and AFM.
The surface functional groups of the fluorescent composite films were characterized using a Nicolet 380 Fourier Transform Infrared spectroscopy (FTIR) (Thermo electron Instruments Co., Ltd., Waltham, MA, USA), in the form of KBr pellets with a resolution of 4 cm −1 . Thermal stability was analyzed in a thermogravimetric analyzer (TGA) (NETZSCH STA 449 F3 Jupiter ® , Selb, Germany), heating from 30 • C to 700 • C at a rate of 10 • C/min in a N 2 atmosphere of 30 mL/min. The zeta potential of DNF and CDs was measured using an SZP-06 Zeta potential analyzer (Mütek, Filderstadt, Germany), and the positive and negative charges of DNF and CDs were analyzed by detecting the zeta potential.

Fluorescence Properties of CDs
As shown in Figure 4, the diluted CDs solution is transparent and clear pale yellow under natural light, and shows an excellent fluorescence effect, with strong blue fluorescence under UV excitation. The CDs have a strong UV-vis absorption feature centered at 292 nm, assigned to the n→π* transition of the surface moieties [37,38]. The phenomenon of emission spectra changing with the excitation wavelength (Figure 5a,b) is similar to that reported by Sun et al. [39]. Surface passivation may generate defect sites on the surface of carbon nanoparticles, and the defect sites can capture radiative excitons, causing fluorescence emissions [16,40,41]. The formation and surface functionalization of carbon nanoparticles occur simultaneously during hydrothermal carbonization. Abundant functional groups, such as carboxylic acids and amino groups, can introduce various defects on Nanomaterials 2022, 12, 2624 5 of 13 the surface, acting as excitation energy holes to induce a fluorescence effect. The results show that the best excitation and emission wavelengths corresponding to the maximum fluorescence intensity are 335 nm and 410 nm, respectively. Interestingly, when the excitation wavelength is higher than 335 nm, the fluorescence emission intensity, at about 530 nm, becomes relatively obvious with the increase in excitation wavelength and the decrease in peak fluorescence intensity, which cannot be ignored at excitation wavelengths over 400 nm. Emission spectra of the CDs solution at the optimal excitation wavelength (335 nm), and excitation spectra at the optimal excitation wavelength (410 nm), are shown in Figure 5c. It can be seen that there is a large energy difference between the excitation peak and the emission peak, and the quantum yield of CDs is calculated to be about 35%.
carbon nanoparticles, and the defect sites can capture radiative excitons, causing fluorescence emissions [16,40,41]. The formation and surface functionalization of carbon nanoparticles occur simultaneously during hydrothermal carbonization. Abundant functional groups, such as carboxylic acids and amino groups, can introduce various defects on the surface, acting as excitation energy holes to induce a fluorescence effect. The results show that the best excitation and emission wavelengths corresponding to the maximum fluorescence intensity are 335 nm and 410 nm, respectively. Interestingly, when the excitation wavelength is higher than 335 nm, the fluorescence emission intensity, at about 530 nm, becomes relatively obvious with the increase in excitation wavelength and the decrease in peak fluorescence intensity, which cannot be ignored at excitation wavelengths over 400 nm. Emission spectra of the CDs solution at the optimal excitation wavelength (335 nm), and excitation spectra at the optimal excitation wavelength (410 nm), are shown in Figure  5c. It can be seen that there is a large energy difference between the excitation peak and the emission peak, and the quantum yield of CDs is calculated to be about 35%.  carbon nanoparticles, and the defect sites can capture radiative excitons, causing fluorescence emissions [16,40,41]. The formation and surface functionalization of carbon nanoparticles occur simultaneously during hydrothermal carbonization. Abundant functional groups, such as carboxylic acids and amino groups, can introduce various defects on the surface, acting as excitation energy holes to induce a fluorescence effect. The results show that the best excitation and emission wavelengths corresponding to the maximum fluorescence intensity are 335 nm and 410 nm, respectively. Interestingly, when the excitation wavelength is higher than 335 nm, the fluorescence emission intensity, at about 530 nm, becomes relatively obvious with the increase in excitation wavelength and the decrease in peak fluorescence intensity, which cannot be ignored at excitation wavelengths over 400 nm. Emission spectra of the CDs solution at the optimal excitation wavelength (335 nm), and excitation spectra at the optimal excitation wavelength (410 nm), are shown in Figure  5c. It can be seen that there is a large energy difference between the excitation peak and the emission peak, and the quantum yield of CDs is calculated to be about 35%.

Morphology of DNF and CDs
DNF is rod-shaped, with a length of 100-300 nm and a diameter of 5-15 nm, and the average values are 220 nm and 11 nm, respectively ( Figure 6). Figure 7 shows the 2D and 3D images of CDs. CDs are spherical nanoparticles with diameters of 3-10 nm. The Zeta potentials of DNF and CDs were −32.6 mV and 77.6 mV, respectively. The higher the absolute value of Zeta potential, the stronger the electrostatic repulsion, which is conducive to better particle dispersion in water and to lower agglomeration. The higher Zeta potential of CDs is due to the presence of amino groups on the surface, indicating that the hydrothermal carbonization of chitosan is an effective method to synthesize amino functionalized fluorescent carbon dots.

Morphology of DNF and CDs
DNF is rod-shaped, with a length of 100-300 nm and a diameter of 5-15 nm, and the average values are 220 nm and 11 nm, respectively ( Figure 6). Figure 7 shows the 2D and 3D images of CDs. CDs are spherical nanoparticles with diameters of 3-10 nm. The Zeta potentials of DNF and CDs were −32.6 mV and 77.6 mV, respectively. The higher the absolute value of Zeta potential, the stronger the electrostatic repulsion, which is conducive to better particle dispersion in water and to lower agglomeration. The higher Zeta potential of CDs is due to the presence of amino groups on the surface, indicating that the hydrothermal carbonization of chitosan is an effective method to synthesize amino functionalized fluorescent carbon dots.

Iron Ion Responsiveness of CDs
As shown in Figure 8a, the fluorescence-quenching effect of Fe 3+ on CDs was analyzed using the emission spectra of CDs solution at Ex = 335 nm, with the addition of 1 mL Fe 3+ solution of different concentrations (0-100 μmol/mL), to 1 mL CDs solution. According to the calculation of the ratio P/P0 of the fluorescence intensity of the CDs solution with different Fe 3+ additions to that without Fe 3+ ions, the fluorescence-quenching of the CDs was analyzed, as shown in Figure 8b. With a trace amount of Fe 3+ ions (5 μmol), the fluorescence intensity of the CDs solution decreased to 30% of the original solution. When Fe 3+ content was more than 30 μmol, the fluorescence effect of the CDs solution almost

Morphology of DNF and CDs
DNF is rod-shaped, with a length of 100-300 nm and a diameter of 5-15 nm, and the average values are 220 nm and 11 nm, respectively ( Figure 6). Figure 7 shows the 2D and 3D images of CDs. CDs are spherical nanoparticles with diameters of 3-10 nm. The Zeta potentials of DNF and CDs were −32.6 mV and 77.6 mV, respectively. The higher the absolute value of Zeta potential, the stronger the electrostatic repulsion, which is conducive to better particle dispersion in water and to lower agglomeration. The higher Zeta potential of CDs is due to the presence of amino groups on the surface, indicating that the hydrothermal carbonization of chitosan is an effective method to synthesize amino functionalized fluorescent carbon dots.

Iron Ion Responsiveness of CDs
As shown in Figure 8a, the fluorescence-quenching effect of Fe 3+ on CDs was analyzed using the emission spectra of CDs solution at Ex = 335 nm, with the addition of 1 mL Fe 3+ solution of different concentrations (0-100 μmol/mL), to 1 mL CDs solution. According to the calculation of the ratio P/P0 of the fluorescence intensity of the CDs solution with different Fe 3+ additions to that without Fe 3+ ions, the fluorescence-quenching of the CDs was analyzed, as shown in Figure 8b. With a trace amount of Fe 3+ ions (5 μmol), the fluorescence intensity of the CDs solution decreased to 30% of the original solution. When Fe 3+ content was more than 30 μmol, the fluorescence effect of the CDs solution almost

Iron Ion Responsiveness of CDs
As shown in Figure 8a, the fluorescence-quenching effect of Fe 3+ on CDs was analyzed using the emission spectra of CDs solution at Ex = 335 nm, with the addition of 1 mL Fe 3+ solution of different concentrations (0-100 µmol/mL), to 1 mL CDs solution. According to the calculation of the ratio P/P 0 of the fluorescence intensity of the CDs solution with different Fe 3+ additions to that without Fe 3+ ions, the fluorescence-quenching of the CDs was analyzed, as shown in Figure 8b. With a trace amount of Fe 3+ ions (5 µmol), the fluorescence intensity of the CDs solution decreased to 30% of the original solution. When Fe 3+ content was more than 30 µmol, the fluorescence effect of the CDs solution almost disappeared, indicating that the obtained CDs have sensitivity and high selectivity to Fe 3+ ions. disappeared, indicating that the obtained CDs have sensitivity and high selectivity to Fe 3+ ions.

Mechanical Properties of Composite Films
It can be seen from Figure 9 that the tensile stress and strain of the composite film first increases and then decreases with the increase in the addition of CDs. When mCDs/mDNF was 1%, the tensile stress of the fluorescent composite film reached the maximum of 70.4 MPa, which was two times higher than that without CDs (35.1 MPa), and the strain was 13.1%. This is mainly because, with the increase in CDs' dosage, the chemical crosslinking degree of DNF and CDs derived from the Schiff base bond increases, and the interaction between them is enhanced, thereby significantly improving the tensile strength of the composite films. When mCDs/mDNF was higher than 1%, excessive CDs led to an increase in the probability of collision between them, which caused some CDs to agglomerate through hydrogen bonds, thus reducing the mechanical properties of composite films. Therefore, an appropriate quantity of CDs can effectively improve the mechanical strength of the fluorescent composite films.

Mechanical Properties of Composite Films
It can be seen from Figure 9 that the tensile stress and strain of the composite film first increases and then decreases with the increase in the addition of CDs. When m CDs /m DNF was 1%, the tensile stress of the fluorescent composite film reached the maximum of 70.4 MPa, which was two times higher than that without CDs (35.1 MPa), and the strain was 13.1%. This is mainly because, with the increase in CDs' dosage, the chemical crosslinking degree of DNF and CDs derived from the Schiff base bond increases, and the interaction between them is enhanced, thereby significantly improving the tensile strength of the composite films. When m CDs /m DNF was higher than 1%, excessive CDs led to an increase in the probability of collision between them, which caused some CDs to agglomerate through hydrogen bonds, thus reducing the mechanical properties of composite films. Therefore, an appropriate quantity of CDs can effectively improve the mechanical strength of the fluorescent composite films.

Mechanical Properties of Composite Films
It can be seen from Figure 9 that the tensile stress and strain of the composite film first increases and then decreases with the increase in the addition of CDs. When mCDs/mDNF was 1%, the tensile stress of the fluorescent composite film reached the maximum of 70.4 MPa, which was two times higher than that without CDs (35.1 MPa), and the strain was 13.1%. This is mainly because, with the increase in CDs' dosage, the chemical crosslinking degree of DNF and CDs derived from the Schiff base bond increases, and the interaction between them is enhanced, thereby significantly improving the tensile strength of the composite films. When mCDs/mDNF was higher than 1%, excessive CDs led to an increase in the probability of collision between them, which caused some CDs to agglomerate through hydrogen bonds, thus reducing the mechanical properties of composite films. Therefore, an appropriate quantity of CDs can effectively improve the mechanical strength of the fluorescent composite films.

Optical Performance Analysis of Composite Films
As shown in Figure 10a, compared with the F-0.2 film, the light transmittance of the F-1 composite film with CDs declines in the visible light region. This may be because, as the content of CDs increases, more Schiff base bonds are formed between CDs and Nanomaterials 2022, 12, 2624 8 of 13 DNF, and the internal structure of the composite film is more compact. The reduced transmittance of F-1 film is still higher than 85%, indicating that the composite film can maintain high transparency in the presence of CDs, and it therefore has application potential as a transparent luminous film. Figure 10b shows the emission spectra of composite films F-0.2 and F-1 at Ex = 335 nm. With increasing CDs content, the fluorescence intensity of the composite film increases slightly, which enhances the composite film as a luminescent film. The changing trend of the fluorescence intensity of the composite film F-1 over time (placed at room temperature without UV light), is shown in Figure 10c. The fluorescence intensity of F-1 film had almost no change one month later, indicating that the composite film has excellent fluorescence stability.

Optical Performance Analysis of Composite Films
As shown in Figure 10a, compared with the F-0.2 film, the light transmittance of the F-1 composite film with CDs declines in the visible light region. This may be because, as the content of CDs increases, more Schiff base bonds are formed between CDs and DNF, and the internal structure of the composite film is more compact. The reduced transmittance of F-1 film is still higher than 85%, indicating that the composite film can maintain high transparency in the presence of CDs, and it therefore has application potential as a transparent luminous film. Figure 10b shows the emission spectra of composite films F-0.2 and F-1 at Ex = 335 nm. With increasing CDs content, the fluorescence intensity of the composite film increases slightly, which enhances the composite film as a luminescent film. The changing trend of the fluorescence intensity of the composite film F-1 over time (placed at room temperature without UV light), is shown in Figure 10c. The fluorescence intensity of F-1 film had almost no change one month later, indicating that the composite film has excellent fluorescence stability.

Morphology of Composite Films
As shown in Figure 11, F-1 film displayed a more compact structure than F-0 film, contributing to the stronger mechanical properties of F-1 film. The Schiff base bond between CDs and DNF increases the internal network density of the composite film, and also creates more intermolecular forces to form more cross-linking sites, which, in turn, affect the pore structure, pore size and distribution of the film [42]. The AFM image and surface roughness parameter of F-1 film are shown in Figure 12. The surface of F-1 film was smooth, with the root square roughness (Sq) of 3.92 nm [34,43]. The surface kurtosis (Sku) of the composite film exceeded 3, implying that the surface morphology distribution is concentrated and the surface structure was relatively uniform [44]. DNF and CDs are well cross-linked in the F-1 film and are evenly distributed without agglomeration to show good compatibility.

Morphology of Composite Films
As shown in Figure 11, F-1 film displayed a more compact structure than F-0 film, contributing to the stronger mechanical properties of F-1 film. The Schiff base bond between CDs and DNF increases the internal network density of the composite film, and also creates more intermolecular forces to form more cross-linking sites, which, in turn, affect the pore structure, pore size and distribution of the film [42]. The AFM image and surface roughness parameter of F-1 film are shown in Figure 12. The surface of F-1 film was smooth, with the root square roughness (Sq) of 3.92 nm [34,43]. The surface kurtosis (Sku) of the composite film exceeded 3, implying that the surface morphology distribution is concentrated and the surface structure was relatively uniform [44]. DNF and CDs are well cross-linked in the F-1 film and are evenly distributed without agglomeration to show good compatibility.

Optical Performance Analysis of Composite Films
As shown in Figure 10a, compared with the F-0.2 film, the light transmittance of the F-1 composite film with CDs declines in the visible light region. This may be because, as the content of CDs increases, more Schiff base bonds are formed between CDs and DNF, and the internal structure of the composite film is more compact. The reduced transmittance of F-1 film is still higher than 85%, indicating that the composite film can maintain high transparency in the presence of CDs, and it therefore has application potential as a transparent luminous film. Figure 10b shows the emission spectra of composite films F-0.2 and F-1 at Ex = 335 nm. With increasing CDs content, the fluorescence intensity of the composite film increases slightly, which enhances the composite film as a luminescent film. The changing trend of the fluorescence intensity of the composite film F-1 over time (placed at room temperature without UV light), is shown in Figure 10c. The fluorescence intensity of F-1 film had almost no change one month later, indicating that the composite film has excellent fluorescence stability.

Morphology of Composite Films
As shown in Figure 11, F-1 film displayed a more compact structure than F-0 film, contributing to the stronger mechanical properties of F-1 film. The Schiff base bond between CDs and DNF increases the internal network density of the composite film, and also creates more intermolecular forces to form more cross-linking sites, which, in turn, affect the pore structure, pore size and distribution of the film [42]. The AFM image and surface roughness parameter of F-1 film are shown in Figure 12. The surface of F-1 film was smooth, with the root square roughness (Sq) of 3.92 nm [34,43]. The surface kurtosis (Sku) of the composite film exceeded 3, implying that the surface morphology distribution is concentrated and the surface structure was relatively uniform [44]. DNF and CDs are well cross-linked in the F-1 film and are evenly distributed without agglomeration to show good compatibility.

FTIR
In order to further explore the formation mechanism of the internal network of the composite film, the infrared spectra are shown in Figure 13, which shows that F-1 composite film has the characteristic absorption peaks of both DNF and CDs. The absorption peak at 1722 cm −1 is attributed to the stretching vibration of C=O of DNF and the peaks around 1060 cm −1 belong to the C-O stretching vibration of DNF and CDs. The peaks at 2800-2900 cm −1 can be assigned to the stretching vibration of C-H. The peaks near 3404 cm −1 correspond to the stretching vibration of O-H and N-H, while that of F-1 exhibits a redshift, which may be caused by the formation of Schiff base bonds. The strong absorption peak at 1545 cm −1 in F-1 film spectrum is attributed to the stretching vibration of the Schiff base bond (C=N) [45], which confirms the covalent bonding of DNF and CDs in the composite film, and is beneficial in improving the mechanical properties of the fluorescent composite film.

FTIR
In order to further explore the formation mechanism of the internal network of the composite film, the infrared spectra are shown in Figure 13, which shows that F-1 composite film has the characteristic absorption peaks of both DNF and CDs. The absorption peak at 1722 cm −1 is attributed to the stretching vibration of C=O of DNF and the peaks around 1060 cm −1 belong to the C-O stretching vibration of DNF and CDs. The peaks at 2800-2900 cm −1 can be assigned to the stretching vibration of C-H. The peaks near 3404 cm −1 correspond to the stretching vibration of O-H and N-H, while that of F-1 exhibits a redshift, which may be caused by the formation of Schiff base bonds. The strong absorption peak at 1545 cm −1 in F-1 film spectrum is attributed to the stretching vibration of the Schiff base bond (C=N) [45], which confirms the covalent bonding of DNF and CDs in the composite film, and is beneficial in improving the mechanical properties of the fluorescent composite film.

FTIR
In order to further explore the formation mechanism of the internal network of the composite film, the infrared spectra are shown in Figure 13, which shows that F-1 composite film has the characteristic absorption peaks of both DNF and CDs. The absorption peak at 1722 cm −1 is attributed to the stretching vibration of C=O of DNF and the peaks around 1060 cm −1 belong to the C-O stretching vibration of DNF and CDs. The peaks at 2800-2900 cm −1 can be assigned to the stretching vibration of C-H. The peaks near 3404 cm −1 correspond to the stretching vibration of O-H and N-H, while that of F-1 exhibits a redshift, which may be caused by the formation of Schiff base bonds. The strong absorption peak at 1545 cm −1 in F-1 film spectrum is attributed to the stretching vibration of the Schiff base bond (C=N) [45], which confirms the covalent bonding of DNF and CDs in the composite film, and is beneficial in improving the mechanical properties of the fluorescent composite film. Figure 13. FTIR spectra of CD, DNF and the composite film F-1. Figure 13. FTIR spectra of CD, DNF and the composite film F-1. Figure 14 shows the thermogravimetry (TG) and derivative thermogravimetry (DTG) curves of F-0 film and F-1 film. For F-1 film, the onset temperature of thermal decomposition is 262 • C, the temperature at the maximum weight loss rate is 316 • C, and the residual mass is 22.1%. the corresponding values of the F-0 film are lower than those of F-1 film, being 238 • C, 296 • C and 20.3%, respectively. This phenomenon illustrates the fact that the thermal stability of the F-1 film is enhanced compared to that of F-0 film. This is due to the chemical cross-linking and hydrogen bonding between CDs and DNF, which is conducive to a denser internal structure and better thermal stability of the fluorescent composite film. Figure 14 shows the thermogravimetry (TG) and derivative thermogravimetry (DTG) curves of F-0 film and F-1 film. For F-1 film, the onset temperature of thermal decomposition is 262 °C, the temperature at the maximum weight loss rate is 316 °C, and the residual mass is 22.1%. the corresponding values of the F-0 film are lower than those of F-1 film, being 238 °C, 296 °C and 20.3%, respectively. This phenomenon illustrates the fact that the thermal stability of the F-1 film is enhanced compared to that of F-0 film. This is due to the chemical cross-linking and hydrogen bonding between CDs and DNF, which is conducive to a denser internal structure and better thermal stability of the fluorescent composite film.

Responsiveness of Fluorescent Composite Film to Fe 3+
Based on the interaction between Fe 3+ and fluorescent CDs, the sensitivity of the F-1 film to Fe 3+ was investigated. An Fe 3+ solution (0-150 μM) was prepared to observe the effect of different concentrations of Fe 3+ ions on the fluorescence intensity of the composite film. According to the fluorescence-quenching degree, the change in the fluorescence intensity of the composite films with different concentrations of Fe 3+ ions was linearly fitted, and the regression curve is shown in Figure 15. The fluorescence quenching efficiency of F-1 film shows a linear response to Fe 3+ ions in the concentration range of 0-100 μM, indicating that the fluorescence intensity of the composite film can be effectively quenched by adding Fe 3+ ions. The calibration curve can be expressed as Y = 0.00638 + 0.0028 X. The limit of detection (LOD) of Fe 3+ is calculated to be 9.09 nM, using the 3σ criterion, which is lower than the detection limits of most other research [46][47][48][49][50]. Therefore, the fluorescence composite film has a high sensitivity to Fe 3+ and can be applied to detect trace Fe 3+ in the field of sensing and environmental monitoring.

Responsiveness of Fluorescent Composite Film to Fe 3+
Based on the interaction between Fe 3+ and fluorescent CDs, the sensitivity of the F-1 film to Fe 3+ was investigated. An Fe 3+ solution (0-150 µM) was prepared to observe the effect of different concentrations of Fe 3+ ions on the fluorescence intensity of the composite film. According to the fluorescence-quenching degree, the change in the fluorescence intensity of the composite films with different concentrations of Fe 3+ ions was linearly fitted, and the regression curve is shown in Figure 15. The fluorescence quenching efficiency of F-1 film shows a linear response to Fe 3+ ions in the concentration range of 0-100 µM, indicating that the fluorescence intensity of the composite film can be effectively quenched by adding Fe 3+ ions. The calibration curve can be expressed as Y = 0.00638 + 0.0028 X. The limit of detection (LOD) of Fe 3+ is calculated to be 9.09 nM, using the 3σ criterion, which is lower than the detection limits of most other research [46][47][48][49][50]. Therefore, the fluorescence composite film has a high sensitivity to Fe 3+ and can be applied to detect trace Fe 3+ in the field of sensing and environmental monitoring.

Conclusions
Based on the self-passivation function of chitosan, chitosan CDs with good fluorescent properties were synthesized using the microwave-hydrothermal method without passivators and strong acid solvents. This strategy is low-cost and environmentally friendly, which provides a new method for the large-scale preparation of highly aminated fluorescent CDs. The quantum yield of CDs is up to 35%, and their fluorescence quenching effect on Fe 3+ is sensitive. A fluorescent composite film with high strength and high trans-

Conclusions
Based on the self-passivation function of chitosan, chitosan CDs with good fluorescent properties were synthesized using the microwave-hydrothermal method without passivators and strong acid solvents. This strategy is low-cost and environmentally friendly, which provides a new method for the large-scale preparation of highly aminated fluorescent CDs. The quantum yield of CDs is up to 35%, and their fluorescence quenching effect on Fe 3+ is sensitive. A fluorescent composite film with high strength and high transparency was constructed based upon the Schiff base reaction between CDs and DNF. The existence of CDs can improve the mechanical properties and thermal stability of the composite film. Owing to the sensitive linear response to Fe 3+ concentration, the composite film can be used in the quantitative analysis and detection of Fe 3+ in environmental monitoring and biosensing fields.