The traditional ethnic herb Tadehagi triquetrum from China: a review of its phytochemistry and pharmacological activities

Abstract Context Tadehagi triquetrum (Linn.) Ohashi (Fabaceae) (TT), is a traditional herbal medicine used especially in China’s ethnic-minority communities, such as the Zhuang, Dai, Li and Wa aeras. As an ethnic medicine, it has long been used to treat various diseases. Objective This review summarised the phytochemical and pharmacological progress on TT from 1979 to October, 2021 by highlighting its chemical classification, structural features, pharmacological applications and folk applications to provide inspirations and suggestions for accelerating further research of this traditional phytomedicine. Methods The information on TT in this article has been obtained using these multiple scientific databases including Scifinder, Web of Science, ScienceDirect, Wiley, ACS publications, Springer, PubMed, China Knowledge Resource Integrated Database from the China National Knowledge Infrastructure (CNKI), Google Scholar and Baidu Scholar. Some information was also collected from classic literature on traditional Chinese medicines. Results More than 70 compounds have been isolated and reported from TT to date by the comprehensive analysis of the current literature. A large number of traditional uses and pharmacological studies have exhibited diversified bioactivities of various TT extracts and its metabolites, including anti-inflammatory, antimicrobial, anti-hepatitis B virus, hepatoprotective, insecticidal, etc. Conclusions As a famous traditional medicine with a long history, TT has various medicinal uses and some of them have been supported by modern pharmacological researches. Further detailed studies on the action mechanisms, pharmacodynamics and structure-function relationships of single compounds or active constituents from TT are also required.


Introduction
The genus Tadehagi (Fabaceae), which consists of 6 species in the world, is mainly distributed in tropical Asia, Pacific Islands and Australia. Two of these plants growing only in China, namely Tadehagi triquetrum (Linn.) Ohashi and Tadehagi pseudotriquetrum (DC.) Ohashi, distributed widely in the southern tropical and subtropical regions of China, such as Guangxi, Guangdong, Hainan, Yunnan, and Hunan province (Wang et al. 2007). Tadehagi triquetrum is called 'Hu-Lu-Cha' and it is locally known as 'Tian-Dao-Bing' (Guangxi province), 'Bai-Lao-She' (Guangdong province), 'Niu-Chong-Cao' (Hainan province), 'Lan-Gou-She' (Jiangxi province), 'Cha-Bao' (Zhuang name) in Chinese. So far, according to 'The Plant List', T. triquetrum has 7 accepted synonyms (Table 1). It is a deciduous subshrub and grows on sunny barren slopes, undergrowth, and hilly areas and in thickets along the road with an altitude of 500-1400 m (Editorial Board of Chinese Flora, Chinese Academy of Sciences 1997). It grows 1-2 m tall and has erect stems with triangular prisms and sparsely short bristles. Its flowering period is from July to August and the fruiting period ranges from October to November. In addition, the microstructural characteristics of TT are mainly shown in the cross sections of the stems and leaves. (Jiangsu College of New Medicine 2000).
TT has been extensively used in traditional medicine. It was recorded for the first time in 'Sheng Cao Yao Xing Bei Yao' and the whole herb of TT has been included in the 1977 Edition of the Chinese Pharmacopoeia. According to the theory of traditional Chinese medicine (TCM), it has bitter taste and the property of cool, which possesses the effects of clearing heat, detoxifying, draining dampness, eliminating phlegm, expelling parasites and dispersing blood stasis. It has long been used to treat colds, acute tonsillitis, acute pharyngitis, hookworm disease, lung abscess, nephritis edoema, dysentery, jaundice, rheumatic arthritis and scabies in the form of a single herb or herbal preparation formula . In particular, people in the Zhuang minority areas of Guangxi province have long used it to treat hepatitis (Peng et al. 2008). In addition, TT is considered an edible herb as the famous non-camellia tea, called 'Hu-Lu-Cha', especially in Guangdong and Guangxi regions of China (Chen and Mei 2013). Its safety, as the dietary herbal medicine, makes it more common in people's daily life, with the enhancement of health awareness. These above therapic effects have been evident in several official ethnic pharmacography, folk applications and public praise. This 'evidence-based' therapeutic history and effects are reflected in the extensive traditional clinical application of TT or various TT formulations that are commonly sold as over-the-counter drugs or dietary supplements.
Previous phytochemical research on TT has revealed the presence of more than 70 secondary metabolites, including flavonoids, phenylpropanoids, phenolic compounds, triterpenoids, steroids and others. Modern pharmacological studies have exhibited diversified pharmacological activities of various TT extracts and these metabolites, such as anti-inflammatory, antimicrobial, anti-hepatitis B virus, hepatoprotective, insecticidal, etc. Given the above, TT has attracted more attention in recent years. As a precious remedial heritage of Chinese medicines, TT deserves to be further explored. However, no review of this research on the whole has been presented to date.
Therefore, in this present review, the phytochemistry and pharmacological effects on TT, have been comprehensively summarised to provide a solid base for further research. Such a systematic review on TT may be valuable for promoting its development into modern pharmaceuticals and improving its clinical uses.

Methodology
This literature review of Tadehagi triquetrum was conducted from 1979 to October, 2021, on information from multiple popular electronic databases, including Science Direct, PubMed, Wiley, Google Scholar, ACS publications, SpringerLink, and China National Knowledge Internet (CNKI). Plant taxonomy was verified by 'iPlant' database (http://www.iPlant.cn). Additional information was also collected from Chinese herbal medicine books (in our school library), PhD and MSc theses (downloaded from CNKI), read manually and analysed in groups. Key words used in the literature search were: Tadehagi triquetrum, phytochemistry, pharmacology, pharmaceutical activities, chemical constituents, and other related search terms. Relevant reports and articles that appeared in some news media or newspapers, as well as literature that was not published in formal professional magazines and periodicals, have been excluded.

Flavonoids
Flavonoids are one of the most common secondary metabolites in nature and have been found to possess numerous biological activities, which are known as possessing the C6-C3-C6 skeleton. They can be divided into many structure types based on the differences in the central C ring. Among all the constituents reported in this plant, flavonoids are the most abundant and have exhibited anthelminthic bioactivity. Up to now, 30 flavonoids (1-30) ( Figure 2) have been isolated and identified from this plant (Wen et al. 1999(Wen et al. , 2000Xiang et al. 2005;Zhang, Cheng, et al. 2011;Zhou et al. 2013;Wu et al. 2014;Jin et al. 2015;Hu et al. 2017;Unni Jayaram et al. 2020) including flavones (1-2), flavonols and their glycosides (1-14), flavanonols (15-17), flavanone (19,20), one flavan-3-ol (18), isoflavonoids (21-30). Some of them exist as flavonoid glycosides and the aglycones in all of these glycosides are derived from flavonols and Hedysarum triquetrum L. 5 Meibomia triquetra (L.) Kuntze 6 Pteroloma triquetrum (L.) Benth. 7 Tadehagi triquetrum subsp. triquetrum flavanones, which are mainly formed by the glycosylation of glucose, rhamnose and galactose. The C-5 and C-7 of TT flavonoids are oxidised in the form of hydroxylation or glycosylation. Investigation on the ethanol extract of the whole plant using bioactivity-guided isolation method led to the discovery of anthelminthic activity of three isoflavonoids (22, 24, 25) (Xiang et al. 2005). Isoflavonoids are very narrow in distribution and are very rare in other families of plants, except the Fabaceae. They are usually a major group of phytoestrogens possessing a wide variety of biological activities. Fabaceae plants are famous for their isoflavonoids, which are almost restricted to them. The structural feature of these isoflavonoids from TT is that they almost have one or two isoprenoid units . Compound 30 is considered as the first bi-isoflavanone possessing a C-3/C-3 000 linkage, which is an extremely rare carbon-carbon linked natural flavonoid dimer.

Phenylpropanoids
Phenylpropanoids are also major compounds and active constituents in TT. Nineteen phenylpropanoids (31-49) have been isolated and identified from TT (Xiang et al. 2005;Zhou et al. 2013;Jin et al. 2015;Wu et al. 2015;Hu et al. 2017), most of which exist in the glycoside form and have been called tadehaginoside derivatives ( Figure 3). These tadehaginoside derivatives generally contain three parts: a phloroglucinol, a glucosyl and a p-hydroxycinnamoyl. Such compounds were first reported in 2000 (Wen et al. 2000). These four compounds (31-34) from TT 0 s aerial part are rare dimeric tadehaginoside derivatives, which have a unusual bicyclo [2.2.2] octene skeleton or cyclobutene ring (Zhang et al. 2016). The plausible biogenetic pathway of their dimerisation could be through a [2 þ 2] cyclisation reaction. In screening for hypoglycaemic activity, these above dimers could stimulate glucose uptake in C2C12 mouse skeletal muscle myotubes. It is worth noting that phloroglucinol and its derivatives have been widely reported due to their structural novelty and the diversity of bioactivities. Clinically, it is used as nonatropinic antispasmodic agent, which can directly act on smooth muscle

Phenolic compounds
Up to now (Figure 4), eight phenolic compounds (50-57) have been identified from TT (Lv et al. 1995;Jin et al. 2015;Zhang et al. 2016;Hu et al. 2017). Many of these constituents possess multiple hydroxyl groups and are called polyphenols, most of which are weakly acidic and are modified by methoxylation and glycosylation or oxidisation. Due to the presence of hydroxyl groups, these phenolic compounds are considered to be major contributors to the antioxidant activity of TT.

Triterpenoids
Five triterpenoids ( Figure 5) have been isolated from TT, which are pentacyclic triterpenes including three skeletons: friedelane, ursane, and lupane type. Friedelane-type triterpenoids (58-59) from TT 0 s stems part are lipid-soluble component and they can be crystallised in solvents with low polarity such as petroleum ether mixed with chloroform (Yang et al. 1989). The latter three compounds (60-62) have increased their hydrophilicity due to the presence of carboxyl groups which could be converted from methyl oxidation at C-4 or C-28 (Wen et al. 2000;Xiang et al. 2005).

Miscellaneous compounds
In addition, several miscellaneous compounds ( Figure 6

Pharmacology
Modern pharmacological studies and plentiful clinical research literature have indicated that various extracts and purified compounds from TT had extensive range of biological activities.

Anti-inflammatory effects
The animal models of inflammation were performed by carrageenan-stimulated rats. An ethanol extract from the leaves of TT was evaluated with measuring the paw volumes at 1 h intervals for 3 h and comparing with control groups. The results suggested it at all the doses (100, 200, and 300 mg/kg) could significantly decrease paw volume compared to control (p < 0.05) and the maximum inhibition of paw edoema was found at 60 min in the 300 mg/kg dose group, which were comparable with standard drug diclofenac sodium. In addition, over-production of nitric oxide (NO) indicates tissue damages. It from 25 to 75 lg/mL exhibited moderate inhibitory of NO in a dose dependent manner. Besides, the carrageenan administration process also could result in increasing in cyclic adenosine monophosphate (AMP)-phosphodiesterase. In a phosphodiesterase activity test, It showed better inhibitory effect against cyclic AMP phosphodiesterase than the positive control, mefloquine. (Kalyani et al. 2011).

Hepatoprotective effects
Hepatopathy is featured by liver dysfunction and subsequent complications through inflammatory and oxidative stress occurred in hepatocytes. In China, traditional Chinese medicines (TCMs) including a large number of ethnologic herbs, play an important role in the treatment of various diseases. TT, as an old folk remedy, has been used very popularly by the Zhuang, Wa and Dai ethnic minority people and its long period of clinical application suggests TT has significant therapeutic effects for hepatopathy (Peng et al. 2008). Wa people in the folk often use the herbal prescription with TT as the principal medicine or monarch drug in TCM theory, to cure hepatitis and jaundice, and the TT decoction with stewed pork has been applied to treat hepatitis without jaundice. In vitro experiments on mice, the hepatoprotective effect of 70% ethanol extract of TT was investigated from the aspects of reducing transaminase, resisting oxygen free radicals, inhibiting lipid peroxidation and cytochrome P450 (Tang et al. 2016).   Carbon tetrachloride (CCl 4 ) is considered as a hepatotoxic revulsant by generating reactive species, such as trichloromethyl radical (CCl 3 À ), to destroy hepatocellular cells and undermine the liver functions. The hepatoprotective effect of tadehaginoside (38) isolated from TT 0 s aerial part was investigated using the hepatic injury model of CCl 4 -lesioned rats. The findings showed that it dose-dependently suppressed the cell proliferation of HepG2 cells in vitro, and it significantly lowered the serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), immunoglobulin E (IgE), and leukotriene (LT). The pathological examination suggested the hepatocellular damage was effectively mitigated by its treatment. Moreover, cytochrome c oxidase (COX) mRNA expression in hepatocytes was upregulated (Tang et al. 2020).
With the treatment of 38, the levels of nuclear factor E2related factor 2 (-Nrf2) and Kelch-like ECH-associated protein 1 (Keapl) were progressively increased, and the downstream enzymes consisting of c-glutamylcysteine synthetase (c-GCS), glutathione (GSH), and catalase (CAT), were activated and their contents in hepatocytes were gradually elevated, which may be involved with the downregulation of tumour necrosis factor alpha (TNF-a) and nuclear factor-kappa B (NF-jB)-expressed protein (Tang et al. 2014). In addition, 38 showed inhibitory effect on hepatic fibrosis induced by CCl 4 in mice and significant downregulation the activities of caspase-3 and caspase-8. These findings demonstrated that 38 from TT could be used to develop an effective hepatoprotective drug (Tang et al. 2017a).

Anti-hepatitis B virus effects
The inhibitory effect against hepatitis B surface antigen (HBsAg) of TT was first reported in 1986, which has no dose dependence (Xie et al. 1986). Previously, there have also been reports of a Chinese herbal decoction consisting of Artemisia capillaris Thunb., TT, Sargentodoxa cuneata R. and Elephantopus scaber L., to cure acute infectious hepatitis. Modern research found compound 38 can suppress the secretion of HBsAg, HBeAg and HBV-DNA from HepG2.215 cells in a dose-dependent manner, and the treated group with 40 lg/mL exhibited highest inhibitory of 83.2% against HBV-DNA, which was better than that of the nucleoside antiviral drug lamivudine (Tang et al. 2017b). Moreover, it can significantly increase the levels of intracellular signal transduction factors STAT1 and STAT2 mRNA.
Gannin formula is used to treat hepatic ascites clinically and has the characteristics of TCM and Zhuang medicine, including eight herbal medicines. Based on TCM theory, Plumbago zeylanica L. and Carapax trionycis were the monarch drugs, Astragalus propinquus Schischkin, Atractylodes macrocephala Koidz, Paeonia lactiflora Pall. and Angelica sinensis (Oliv.) Diels were the minister drugs, TT and Lycopus lucidus Turcz. Ex Benth. were adjuvant drugs (Yang et al. 2017). Cirrhosis ascites is one of the complications after hepatitis B develops into cirrhosis. Ganning formula could alleviate cirrhosis ascites and the remission rate of ascites reached 93.75% in the treatment group and 44.44% in the control group, with a significant different between the two group (p < 0.01) (Qin et al. 2016).

Antidiabetic effects
In the type 2 diabetic mouse model established by intraperitoneal injection of streptozotocin (STZ, 150 mg/kg), the ethyl acetate fraction, n-butanol fraction and 60% ethanol fraction could significantly reduce the level of fasting blood glucose. Of these, 60% ethanol extract of TT 0 s stems and leaves exhibited the most significant hypoglycaemic effect and its hypoglycaemic rate reached 23.61%. Chloroform fraction and petroleum ether fraction had no activity on diabetic mice (Li et al. 2012). The latest study found the n-butanol fraction from TT shown the a-glucosidase inhibitory on yeast and small intestine in mice (He et al. 2020). Therefore, the effective constituent of antidiabetics from TT maybe mainly exist in the n-butanol fraction, and a detailed investigation is necessary to identify the chemical basis which is responsible for its antidiabetic activity. The author utilised n-butanol to extract the 70% ethanol extract of TT 0 s stems and leaves to prepare the polyphenol-rich extract (PRE). It could lower hyperinsulinemia, improve oral glucose tolerance, and reduce hyperlipidaemia and liver fat content (p < 0.05). PRE treatment could increase the content of liver glycogen, and the activity of hepatic glucokinase and pyruvate kinase (p < 0.05). The eight compounds were isolated from PRE based on the bioactivity-guided isolation method. Six polyphenols (compounds 7, 8, 14, 31, 34, 39), particularly rutin (14), increased glucose consumption by hepatocytes, suggesting that polyphenols accounted for the antidiabetic effect of PRE (Lin et al. 2020).
Hyperglycaemia and insulin resistance (IR) are main characteristics of type 2 diabetic (T2DM). The activity of tadehaginosin (49) on consumption of glucose was evaluated on HepG2 cells. In the NBD-glucose (2-NBDG) uptake assay, its activity (10 mM) was comparable to insulin (0.1 mM), indicating a potent activity in stimulating glucose uptake by myotubes. Very interestingly, tadehaginosin (49) showed stronger activity than compound 48, since they belonged to two structurally related phenylpropanoids, one lignan and one cumarin. The results suggested tadehaginosin could be a potential therapeutic strategy for stimulating glucose consumption and be meaningful in treatment of diabetes .
In another study, compound 34 exhibited the hypoglycaemic activity, with an efficacy comparable to that of 100 nM insulin in the model of C2C12 mouse skeletal muscle myotubes (Zhang et al. 2016). Molecular docking results demonstrated that 34 could bind tightly to peroxisome proliferator-activated receptor c (PPARc), a key regulator of glucose homeostasis. A series experiments of molecular biology showed that 34 significantly enhanced the transcriptional activity of PPARc and increased the level of glucose transporter-4 (GLUT-4) protein whose expression was regulated by PPARc. Therefore, the above results indicate compound 34 is regarded as a most prominent compound for the treatment of diabetes.

Anti-gout effects
Zhuang medicines have been proved to effectively relieve clinical symptoms and improve immune function. 'Jinqian Hulu' decoction, as a traditional Zhuang prescription containing TT, has been used clinically in treatment of gout for decades and has achieved good clinical efficacy. Pharmacological studies in vitro have shown that the decoction could reduce the degree of toe swelling in mice with sodium urate injection and inhibit the synthesis of TNF-a and IL-1. Furthermore, it can significantly decrease the level of uric acid in hyperuricaemia mice of yeast intragastric administration, and suppress the activities of xanthine oxidase (XOD) and adenosine deaminase (ADA) which are key enzymes for purine metabolism to produce uric acid and a key link in the regulation of uric acid production (Tan et al. 2016). It is preliminarily shown that 'Jinqian Hulu' decoction has the effect of treating acute gouty arthritis, providing a theoretical basis for its further clinical use.
'Hulucha Chubi' decoction is another empirical formula based on traditional theory of Zhuang medicine, which contains four herbs with TT as the primary medicine. The clinical trial showed that this decoction could improve the symptoms and signs of acute gouty arthritis, which is an acute aseptic inflammatory response whose characteristic is intense pain caused by urate deposition. The results indicated its effect of reducing uric acid was significantly better than that of the control drug Diclofenac sodium Dual Release Eneric-coated Capsules (Temmler Ireland Ltd.) . The results confirmed its effectiveness and the validity of traditional medicine theory, and further provided the basis for its rational clinical application for gout arthritis.

Anti-atherosclerotic effects
Scientific investigations indicated that compounds 35 and 41 significantly reduced oxLDL-induced lipid accumulation and exhibited comparable effects to that of the positive control simvastatin. Compounds 35, 39 and 41 (10 lmol/L) prevented foam cells formation in RAW264.7 cells. And above three compounds could up-regulate of cholesterol efflux-related genes such as ABCA1 and ABCG1, and inhibit the expression of CD36 and SR-1, which are cholesterol influx-related genes. The structureactivity relationship study showed cinnamyl is the most pivotal group of these compounds .

Antihyperlipidemic effects
Tadehaginoside (38) can decrease oleic acid (OA)-elicited neutral lipid accumulation and intracellular contents of total cholesterol and triglyceride in the HepG2 cell model ). Its efficacy was comparable to that of the positive control simvastatin and was not due to its cytotoxicity. The PCR experiment indicated tadehaginoside decreased the transcription of lipogenesis-related transcription factors sterol regulatory element-binding proteins (SREBPs) and peroxisome proliferator-activated receptors (PPARs) .

Wound healing activities
Through clinical trials, Xiao have been used for the treatment of 89 cases of burn wounds and achieved good results from 1980 to 1994, using 'Jiu-Bai-Cha' lotion contained TT, Senecio scandens Buch.-Ham. ex D. Don, Sanguisorba officinalis L., and Bletilla striata (Thumb.) Rchb. f. The clinical observation found that it can relieve the burning pain, promote the blister subside and swelling decrease, prevent re-contamination and promote wound repair. The wounds of first-degree burns cured by this lotion generally did not leave paralysis marks, but only slight changes in the skin's pigment. The lotion can also be used as an oil agent, which has little irritation on the wound surface and is suitable for children burns (Xiao 1995).
In another study, the results showed that the ethanol extract obtained from the leaves of TT could increase collagen deposition and hydroxyproline content in the incision wound model of rat. And the experiment in the excision wound model indicated it can enhance epithelization and collagenation to significantly increase in the rate of wound contraction. Thus, TT could be regarded as a medicinal remedy for wound healing (Shirwaikar et al. 2003).

Antiparasitic and anthelminthic effects
'Quchong Shengxue' pills containing 51.6% TT, were clinically used to treat ancylostomosis with severe anaemia. The results showed that the clinical recovery rate reached 94.29% (Luo 2000).
The expelling effect of 10% of TT against rabbit coccidian was investigated using the McMaster's method, which suggested its effective dose to rabbit coccidia was 0.5-1.3 g/kg. Above the dose of 1.9 g/kg, it caused death in rabbits (Li et al. 2002b). In vitro test, cyclokievitone (23) had remarkable effects on inhibiting or killing coccidian eggs (Li et al. 2003a). The above findings implied its potential in veterinary medicines.

Anti-allergic effects
Allergic asthma is a disease characterised by chronic bronchitis and hyperreactivity of trachea involving multiple inflammatory cells. The 50% acetone extract of TT 0 s stems and leaves exhibited the anti-allergic effect on the rat model of type I allergy. It decreased the contents of IgE, leukotriene and histamine in the serum at the dose of 100 mg/kg. Compared with the positive control group, the treatment group with a dose of 100 mg/kg significantly reduced the number of eosinophilia and the area of inflammation in lung tissue (Zhou et al. 2011).

Anti-fungal and anti-bacterial effects
TT was found to be active in the anti-bacterial test by inhibiting cyclic AMP phosphodiesterase, with the inhibition ratio of 72.58 ± 1.89%, compared to the positive control mefloquine with 45.20 ± 3.30% (Chit et al. 2001). Zhang et al. described that it could be resistant to bacterial toxin . Clinically, the combination of herbs that contains 20% of TT, has been used to treat the candida vaginitis, which had a 76.7% cure rate (Zhang 1979).
Infantile pustules are a common skin infection caused by Staphylococcus aureus. The joint use of TT and mupiroxine could make the cure rate of disease reach 60% (Tian 2013).

Anti-mollusk effects
The crude powder and the decoction of TT had a good effect on the expulsion of Lymnaeidae mainly containing Radix swinhoei and Galba pervia, and the powder exhibited better effect. The powder group with the concentration of 0.5% (g/L) could kill all these molluscs within 17 hours (Li, Li et al. 2003). Besides, the tested extract of TT with the concentration of 1.6% also showed the anti-mullusk activity.
Compounds 46, 67 and 69 showed no activity against Radix swinhoei, which belonged to water-soluble compounds, but cyclokieviton (23) and kaempferol (3) with the concentration from 0.005-0.01% had the activity within 24 hours. Thus, it was suggested that they might be effective components of TT (Li et al. 2003b).

Antioxidative effects
TT is rich in polyphenolic compounds which have been considered to possess antioxidant activities. Because of the availability and the presence of flavonoids and phenolic compounds, 95% ethanol extract of TT's leaves was evaluated for its antioxidant activity in vitro. The extract could decrease significantly the concentration of DPPH-radical and H 2 O 2 , whose effect on scavenging of free radicals was close to the control ascorbic acid. This extract at the concentration of 50 lg/mL had the maximum H 2 O 2 scavenging effect (Kalyani et al. 2011).

Treatment of anorexia and dyspepsia
Infantile anorexia is a common disease in paediatrics, which usually occurs in infants under one year old. The patients mainly have symptoms such as anorexia and fatigue. 'Xiaoer Kang' granule is a kind of Chinese patent medicine including TT (Wang and Zhang 2010). The results from the clinical test showed that the total effective rate and significant efficiency of the treatment group were significantly higher than those of the control group, and the treatment group had a better effect in improving the accompanying symptoms .

Genitourinary function
Gao and Chen used the 'Gongying Hulu' decoction to treat prostate hyperplasia with the syndrome type of moist-heat on bladder in TCM (Gao 2004;Chen 2008). Sun has reported that the total effective rate of treating prostate hyperplasia using the decoction have reached 93.4% (Sun 2003). Meanwhile, the decoction has been used to treat 98 cases with prostate hyperplasia in combination with 'Buzhong Yiqi' pill, and the rate of clinical cure was 32.7% (Wang 1995). Wang and Xu have treated the acute urinary retention caused by prostatic hyperplasia and the effective rate of treatment was 78.6% (Wang and Xu 1998). Professor Jin has used TT to treat various genitourinary diseases .

Insect extermination effect
To verify the traditional record of deworming, its insect extermination effect was investigated towards the larvaes of Tenthredinidae and Arctiidae using its different dosage forms (Li et al. 2004). Since it actually was inactive against these larvae, the reported folk statement of 'removing and killing insects' may be understood as 'expelling' its adults so that they cannot lay eggs in the host, and then it is impossible for them to develop larvae, and another explanation was that the target of expulsion was the parasites.

Other applications
Deng often substitute TT for Artemisia capillaris Thunb., a commonly used traditional Chinese medicine with the effect of reducing dampness and removing jaundice (Jin et al. 2017). Li summarised TT's clinical applications as the main herb, such as treating carbuncle, heat dysentery and malnutrition (Ge and Li 1994). In addition, it can be used as feed additive with the purpose of promoting digestion and gaining weight in a mouse model (Li et al. 2002a).

Conclusions
As a famous traditional medicine with a long history, TT has many medicinal applications. It can be used as a single medicine or in combination with other TCMs in multiple ethnic groups to treat various ailments. Some medicinal uses are clearly recorded in traditional medicine classics and official ethnic literature, while others have been directly used in folk life through generations of oral transmission. Just because of its clinical efficacy and history, more and more researchers are interested in it. So, extensive phytochemistry and pharmacology investigations on TT have been carried out. Therefore, this review described and summarised the detailed chemical constituents and their biological activities of TT, which could provide the foundation for future research. To the best of our knowledge, 73 compounds are present in this plant and mainly include flavonoids, phenylpropanoids, phenolic compounds, triterpenoids and steroids. Among them, some compounds show good pharmacological activities, especially anti-atherosclerotic and antidiabetic activities. Studies indicated the phenylpropanoids in TT had the potential in treatment of diabetes disease, but their structureactivity relationship and the mechanism of action required deeper research. Some studies have found single compounds with novel skeletons, but their activities seemed to be unsatisfactory.
It is particularly worth mentioning that the results of modern pharmacological research do not seem to be as good as it is used in real life. This may involve multiple factors, such as the authenticity of TT, collection time, processing methods, extraction conditions. In particular, the extraction method of TT is decoction in folk clinical application, but in most reports, it is reflux extraction by ethanol. So, it is very necessary to go into the clinic and study its preparation process in advance. Besides, in many cases, its extracts performed better than single compounds, suggesting that these single pure constituents could not well explain the traditional medicinal activities of TT. This may indicate its mechanism of action is related to multiple components. Hence, it is necessary to develop a variety of pharmacological models and methods, such as network pharmacology strategies, to promote the research of multiple components and multiple targets of TCMs.
To be sure, many of pharmacological studies have been conducted with its extract or its formulations, and many of them are based on in vivo studies. But many of the early experiments were very superficial. A relatively large amount of research has focussed on its extracts and even powders, without really finding its effective ingredients. Therefore, the studies based on monomeric compounds are still lacking, especially in terms of activity screening, action mechanism and pharmacodynamics. Furthermore, more clinical trials should be conducted to verify its functions. On the whole, the research is not systematic and in-depth enough, specifically for one therapeutic effect. Of course, suitable pharmacological experimental models in vitro and in vivo, are also very vital and urgent.
In addition, studies on its composition analysis and quality control, such as component fingerprint, have not yet established clear and reliable strategies. In the future, further research is necessary to investigate phytochemical and pharmacological activities, including the deeper molecular mechanism, in order to discover active compounds from TT.