Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m1A22-tRNA methyltransferase TrmK

The enzyme m1A22-tRNA methyltransferase (TrmK) catalyzes the transfer of a methyl group to the N1 of adenine 22 in bacterial tRNAs. TrmK is essential for Staphylococcus aureus survival during infection but has no homolog in mammals, making it a promising target for antibiotic development. Here, we characterize the structure and function of S. aureus TrmK (SaTrmK) using X-ray crystallography, binding assays, and molecular dynamics simulations. We report crystal structures for the SaTrmK apoenzyme as well as in complexes with methyl donor SAM and co-product product SAH. Isothermal titration calorimetry showed that SAM binds to the enzyme with favorable but modest enthalpic and entropic contributions, whereas SAH binding leads to an entropic penalty compensated for by a large favorable enthalpic contribution. Molecular dynamics simulations point to specific motions of the C-terminal domain being altered by SAM binding, which might have implications for tRNA recruitment. In addition, activity assays for SaTrmK-catalyzed methylation of A22 mutants of tRNALeu demonstrate that the adenine at position 22 is absolutely essential. In silico screening of compounds suggested the multifunctional organic toxin plumbagin as a potential inhibitor of TrmK, which was confirmed by activity measurements. Furthermore, LC-MS data indicated the protein was covalently modified by one equivalent of the inhibitor, and proteolytic digestion coupled with LC-MS identified Cys92 in the vicinity of the SAM-binding site as the sole residue modified. These results identify a cryptic binding pocket of SaTrmK, laying a foundation for future structure-based drug discovery.

The enzyme m 1 A22-tRNA methyltransferase (TrmK) catalyzes the transfer of a methyl group to the N1 of adenine 22 in bacterial tRNAs. TrmK is essential for Staphylococcus aureus survival during infection but has no homolog in mammals, making it a promising target for antibiotic development. Here, we characterize the structure and function of S. aureus TrmK (SaTrmK) using X-ray crystallography, binding assays, and molecular dynamics simulations. We report crystal structures for the SaTrmK apoenzyme as well as in complexes with methyl donor SAM and co-product product SAH. Isothermal titration calorimetry showed that SAM binds to the enzyme with favorable but modest enthalpic and entropic contributions, whereas SAH binding leads to an entropic penalty compensated for by a large favorable enthalpic contribution. Molecular dynamics simulations point to specific motions of the C-terminal domain being altered by SAM binding, which might have implications for tRNA recruitment. In addition, activity assays for SaTrmK-catalyzed methylation of A22 mutants of tRNA Leu demonstrate that the adenine at position 22 is absolutely essential. In silico screening of compounds suggested the multifunctional organic toxin plumbagin as a potential inhibitor of TrmK, which was confirmed by activity measurements. Furthermore, LC-MS data indicated the protein was covalently modified by one equivalent of the inhibitor, and proteolytic digestion coupled with LC-MS identified Cys92 in the vicinity of the SAM-binding site as the sole residue modified. These results identify a cryptic binding pocket of SaTrmK, laying a foundation for future structure-based drug discovery.
Enzyme-catalyzed post-transcriptional modifications of tRNA occur in all domains of life, contributing to tRNA stability and folding, proper aminoacylation, and translational fidelity. Among these modifications, methylation is the most abundant and diverse, occurring on both the ribose and nucleobase moieties of tRNA nucleosides at several positions (1)(2)(3). All methylation of tRNA adenine N1 (m 1 A) is found in the core region of the tRNA: m 1 A9, m 1 A14, and m 1 A22 in the D-arm and m 1 A58 in the T-arm (4). The m 1 A22 modification is present only in bacterial tRNA and was first detected in tRNA Tyr from Bacillus subtilis (5). While the biological role of this modification is still elusive, the enzyme responsible for it was identified in 2008, namely m 1 A22-tRNA methyltransferase (TrmK) (6).
TrmK catalyzes the transfer of a methyl group from SAM to tRNA, producing m 1 A22-tRNA and SAH (6) (Fig. 1). The enzyme belongs to the class-I methyltransferase family comprised of an N-terminal domain with a Rossmann-like fold for SAM binding and a C-terminal domain with a novel 4-helix fold (7). The latter is analogous to the tRNA-interacting Cterminal domain of the archaeal m 1 G37-tRNA methyltransferase Trm5 (8) and is proposed to play a similar role in TrmK (9).
While TrmK is not required for growth in B. subtilis (6), it is essential for survival and growth of the pathogenic Grampositive bacteria Staphylococcus aureus (10)(11)(12) and Streptococcus pneumoniae (13). Importantly, disruption of the TrmKencoding gene prevents S. aureus survival not only in rich media but during infection of the bloodstream, vitreous fluid, and cutaneous abscesses in mice (10). Moreover, the TrmKencoding gene is expressed almost constitutively in cell models of infection (14), during acute and chronic osteomyelitis (15), and in the highly infectious and multidrug-resistant USA300 strain of S. aureus during human cutaneous abscess and mouse kidney infection (16). Crucially, TrmK has no homolog in mammals (6), making TrmK a promising target for the development of novel antibiotics against S. aureus. This bacterium is a leading cause of healthcare-associated and community-associated infections worldwide, with many patients infected with methicillin-resistant S. aureus (MRSA). MRSA encompasses strains resistant to most antibiotics in clinical use (17). In Europe, MRSA infections are responsible for an average of 7000 deaths a year, the second highest among all drug-resistant bacterial infections (18), and in the USA, more deaths occur due to MRSA infection than to HIV/AIDS and tuberculosis combined (19). The World Health Organization ranked MRSA as a high priority in its list of bacteria against which novel antibiotics are urgently needed (20).
In the present work, we have cloned and expressed the gene encoding S. aureus TrmK (SaTrmK), purified the recombinant protein, and used X-ray crystallography to obtain highresolution structures of the enzyme. We employed isothermal titration calorimetry (ITC) and differential scanning fluorimetry (DSF) to characterize cofactor binding and performed molecular dynamics (MD) simulations to assess differences in flexibility upon ligand binding. We used a luminescence-based SAH-detection assay and LC-MS to uncover the determinants of SaTrmK activity toward tRNA and small RNA hairpins. Finally, we carried out in-silico screening of compounds and identified a site on SaTrmK amenable to covalent inhibition.

SaTrmK purification and biophysical characterization
SaTrmK was successfully purified to homogeneity with no other bands visible on a Coomassie Blue-stained gel following SDS-PAGE (Fig. S1). A typical yield was 100 mg of SaTrmK per liter of culture. The molecular mass of the protein was confirmed by electrospray-ionization (ESI)/TOF-MS to be 25,564.5 (Fig. S2), in agreement with the predicted molecular mass of 25,565.3. DSF assays yielded, upon data fitting to Equation 2, a melting temperature (T m ) of 40.3 ± 0.1 C, which increased modestly to 41.4 ± 0.1 C and more significantly to 45.9 ± 0.1 C in the presence of SAH and SAM, respectively (Fig. S3). These values suggest SAH binding has little effect on enzyme thermostability. SAM, however, can thermally stabilize the protein upon binding. The SaTrmK elution profile from an analytical gel-filtration column revealed a major peak at 16 ml (MW of 23 kDa) and a minor peak at 14.4 ml (MW of 50 kDa) (Fig. S4). This profile indicates most of the protein is monomeric, while a minor fraction is dimeric.
Crystal structures of SaTrmK apoenzyme, SaTrmK:SAM, and SaTrmK:SAH To shed light on the structural basis for cofactor binding and help inform future structure-based inhibitor design, crystals of SaTrmK apoenzyme, and the binary complexes SaTrmK:SAM and SaTrmK:SAH were obtained, and data were collected to a resolution of 1.3, 1.4, and 1.5 Å, respectively (Table S1). The 2mF o − DF c map showed electron density for SAM and SAH in the respective structures (Fig. 2). SaTrmK is typical of class-I methyltransferases, with an Nterminal domain where the cofactor binds and methyl transfer takes place and a C-terminal domain likely involved in tRNA recognition (8,9). Superposition of the three structures ( Fig. S5) revealed negligible effect of cofactor binding on Cα root-mean-square deviations (RMSDs), whose values were 0.15, 0.18, and 0.08 Å between SaTrmK and SaTrmK:SAM, SaTrmK and SaTrmK:SAH, and SaTrmK:SAM and SaTrmK:SAH, respectively. The SaTrmK apoenzyme structure superposes with the apoenzyme structures of B. subtilis TrmK (Protein Data Bank (PDB) entry 6Q56) (9) and S. pneumoniae TrmK (PDB entry 3KR9) (7), with which it shares, respectively, 44% and 42% amino acid sequence identity, with Cα RMSDs of 0.68 and 0.98 Å, respectively, with the most ostensible differences located in the coiled-coil motif of the C-terminal domain (Fig. S6).
The electron density data for the SaTrmK apoenzyme showed clearly defined density near the active site that could not be fitted with any of the small molecules involved in purification or crystallization of the enzyme, suggesting it was carried through with SaTrmK from protein expression. Intriguingly, citrate could be fitted perfectly into the electron density, making polar contacts with the side chains of His27, Tyr29, and Asn59 (Fig. S7A). It is possible the negatively charged groups of citrate are mimicking the phosphate group(s) of tRNA. DSF analysis indicated that citrate leads to an overall modest decrease in SaTrmK T m (Fig. S7B) that only becomes noticeable at high citrate concentrations, which is incompatible with the presumed tight binding required for the interaction to continue from protein expression to crystallization. Therefore, the significance of this observation, if any, is still elusive. Interestingly, seven tricarboxylic acid cycle intermediates, including citrate, have been shown to regulate the catalytic activity of a nucleic acid-processing enzyme, yeast type-II topoisomerase (21).

The cofactor binding site
The binding site for SAM is restricted to the N-terminal domain, and several polar and nonpolar interactions are made with residues via both main-chain and side-chain moieties to position the cofactor in the active site (Fig. 3A). SAM's COO − group forms a salt bridge with the ω-NH 2 and ωʹ-NH 2 groups of Arg7, while its NH 3 + group donates a hydrogen bond (Hbond) to the main chain CO-groups of Cys92 and Gly24 and to a water molecule (W1) which in turn H-bonds to Asp22. The 2ʹ-OH and 3ʹ-OH form H-bonds with another water molecule (W2) that itself H-bonds to Glu47 side chain COO − and Val48 main chain -NH. Adenine N1 and N6 atoms form H-bonds with Gly76 main chain -NH and Asp75 side chain COO − , respectively. Furthermore, the adenine plane is flanked on both sides by hydrophobic interactions with Met94, Leu98, Ile102, and Val48.
A superposition of the active sites of SaTrmK, SaTrmK:-SAM, and SaTrmK:SAH (Fig. 3B) shows there is local motion of some residues in order to accommodate the cofactor. In the region of the active site which binds the adenine moiety of SAM, the side chain of Glu47 moves slightly from its position in the apoenzyme but retains the same orientation. Interestingly, water molecule W2 is only observed in the structures when SAM and SAH are bound. Val48 moves 1 Å and rotates approximately 120 enabling both methyl groups to face away from SAM, and Asp75 also moves 1 Å and rotates  B, overlay of the cofactor-binding site of SaTrmK apoenzyme and the binary complexes with SAM and SAH. In both panels, oxygen is depicted in red, nitrogen in blue, sulfur in yellow, and carbon in either gray (SAM), teal (residues in SaTrmK:SAM), purple (residues in SaTrmK:SAH), or green (residues in SaTrmK apoenzyme). Water molecules are shown as red spheres. SaTrmK, S. aureus TrmK; TrmK, m1A22-tRNA methyltransferase.
EDITORS' PICK: Staphylococcus aureus m 1 A22-tRNA methyltransferase slightly. Their position in the apoenzyme would have caused a steric clash with SAM. Asp26, which is in the vicinity of the SAM binding site but does not interact with the cofactor, moves significantly upon cofactor binding from its position in the apoenzyme (Fig. S8).

SaTrmK binds SAM and SAH with distinct thermodynamics
In order to confirm binary complex formation in solution as observed in crystallo and gain insight into cofactor binding equilibrium thermodynamics, SAM and SAH binding to SaTrmK were determined by ITC (Fig. 4). The binding isotherms were best fitted to a single-site binding model with 1:1 (protein:ligand) stoichiometry. Binding of SAM to SaTrmK yielded a K D of 39 ± 4 μM, while binding of SAH resulted in a K D of 0.94 ± 0.05 μM. The Gibbs free energies (ΔG) were −5.92 ± 0.07 and −8.09 ± 0.03 kcal/mol for SAM and SAH binding to SaTrmK, respectively, with SAH binding favored by 2.17 ± 0.08 kcal/mol. Interestingly, while binding of both SAM and SAH are exothermic, they are driven by distinct thermodynamic functions. The SaTrmK:SAM complex is stabilized by modest but favorable enthalpic and entropic contributions, with ΔH of −3.46 ± 0.08 kcal/mol and TΔS of 2.5 ± 0.1 kcal/mol. On the other hand, the SaTrmK:SAH complex formation is enthalpically driven, with ΔH of −12.8 ± 0.2 kcal/mol which pays for an entropic penalty on binary complex formation, given a TΔS of −4.7 ± 0.3 kcal/mol.

SAM binding alters specific motions of SaTrmK
To gain insight into the dynamics of SaTrmK, MD simulations were carried out over 500 ns using the crystal structures as starting points (Fig. 5). The distance of the salt bridge between Arg7 and either SAM or SAH confirmed the ligands did not dissociate from the protein over the course of the simulations but showed that the salt bridge with SAM oscillated more in comparison with SAH ( Fig. 5A). Timedependent Cα RMSDs indicated only minor oscillations for the three structures (Fig. 5B), and the overall root-mean-square fluctuations were remarkably similar, with the most flexible region comprising the C-terminal domain (Fig. 5C). Moreover, correlated motions between protein domains are similar among the three structures (Fig. S9).
In order to uncover dominant motions of SaTrmK, principal component analysis was carried out, and the first three eigenvectors were isolated (Fig. 5D). This analysis revealed that the first two eigenvectors are dominated by specific motions of the C-terminal domain, but SAM binding is accompanied by a swap in the specific trajectories contributing to the highest root-mean-square fluctuations, as compared with SaTrmK apoenzyme and SaTrmK:SAH binary complex (Fig. 5E). Furthermore, the third eigenvector suggests that SAM and SAH binding reduces the amplitude of specific motions in the N-terminal domain, most notably those around residues implicated in cofactor binding, while increasing specific motions in the C-terminal domain, as compared with the apoenzyme (Fig. 5, D and E).
Molecular electrostatic potential surfaces were calculated for the three structures throughout the simulations, and snapshots were taken every 125 ns (Fig. S10). While the cofactor binding pocket is consistently dominated by negative charges in all structures, positive charges outside this pocket undergo some redistribution throughout the trajectories, influenced by the presence or absence of SAM or SAH.

SaTrmK-catalyzed methylation of tRNA Leu
The MTase-Glo methyltransferase assay, which detects luminescence generated in a luciferase-coupled reaction driven by ATP, which in turn is synthesized enzymatically from the SAH produced in the methyltransferase reaction (22), was used to quantify the methylation of S. aureus tRNA Leu as catalyzed by SaTrmK. This is a robust and sensitive assay used to characterize RNA methyltransferase activity (23) and to screen tRNA methyltransferase inhibitors (24), as it has been shown to result in lower false-positive rates than other methods (25). In the absence of tRNA, SaTrmK contributes no  background luminescence, as expected since the enzyme is not purified bound to SAH. This is evidenced by the crystal structure of the apoenzyme and no SAH detected by LC-MS after denaturation of SaTrmK which would have released any bound SAH (Fig. S11). Background luminescence possibly originating from contaminating ATP in the RNA samples was drastically diminished by dialysis of both tRNA and small RNA hairpins prior to use, and any residual background luminescence was subtracted from the reactions via controls lacking RNA. An SAH standard curve (Fig. S12) was used for quantification of the methylation reaction.
Activity of SaTrmK was detected when tRNA Leu , which contains an adenine at position 22, was used as substrate, but no SAH formation could be detected when either A22C-tRNA Leu , A22U-tRNA Leu , or A22G-tRNA Leu was tested as substrate ( Fig. 6A), suggesting methylation is conditional on an adenine at position 22. Furthermore, SAH production was linearly dependent on SaTrmK concentration with tRNA Leu as substrate (Fig. 6A, inset). To validate the assay further for detection of SaTrmK enzymatic activity, SAH production was measured in the presence and absence of sinefungin, a SAM analog that is a pan-methyltransferase inhibitor (26). Sinefungin (10 μM) led to 98% inhibition of SAH production (Fig. 6B). In the absence of SaTrmK, sinefungin had no effect on luminescence generated by the addition of SAH to the assay (Fig. S13), pointing to sinefungin preventing SAH production by inhibiting SaTrmK. When the tRNA Leu was hydrolyzed to nucleoside 5ʹ-monophosphates by nuclease P1 following the methylation reaction and subjected to LC-MS analysis, ions with the same mass/charge (m/z) and retention time as those for the N 1 -methyl-AMP standard were detected (Fig. 6C).
However, no such signal was detected when either SaTrmK was omitted from the methylation reaction or A22C-tRNA was used instead of tRNA Leu (Fig. 6C). This is in strict accordance with TrmK producing m 1 A22-tRNA (6). Moreover, by directly detecting the methylated adenine, the LC-MS-based assay provided orthogonal validation of the MTase-Glo Methyltransferase Assay for detection of SaTrmK activity.
SaTrmK-catalyzed methylation of short RNA hairpins B. subtilis TrmK has been reported to require a full-length tRNA for activity, since no methyl transfer was detected when smaller RNAs were tested as substrate (9). To confirm the same was true for SaTrmK, an RNA 18mer , predicted to form a hairpin similar to the D-arm of tRNA but with only four nucleotides in the loop and containing adenine at three positions (Fig. 7A), was designed and tested as a substrate of the enzyme. Surprisingly, SaTrmK accepted this RNA 18mer as a substrate (Fig. A). Catalytic activity increased to a level approaching that detected with tRNA Leu (Fig. 6A) when A10G-RNA 18mer replaced the original RNA 18mer (referred to as WT-RNA 18mer ), but it was undetectable with either A11G-RNA 18mer or U5C/A14G-RNA 18mer as the substrate (Fig. 7A). This suggests that A10G-RNA 18mer can be efficiently methylated by SaTrmK, pointing to a novel property of this enzyme.
The lack of methylation activity toward U5C/A14G-RNA 18mer was puzzling since A14 should be in a Watson-Crick pair with U5 in the WT-RNA 18mer , making its N1 unavailable as a nucleophile for the methyl-transfer reaction, and A11 would be the only expected methylation site in the A10G-RNA 18mer . Hence it is possible the C5 and G14 substitution caused a decrease in affinity for SaTrmK and/or reduction of A11 methylation efficiency. To probe the limits of RNA 18mer methylation further, a second-generation RNA 18mer was synthesized which contained G5, C14, and only one adenine, at position 11 (A11-RNA 18mer ). At higher concentrations of RNA and SaTrmK than previously used, significant methylation of A11-RNA 18mer was detected (Fig. 7B). To confirm SAH formation was indeed a result of SaTrmKcatalyzed methylation of A11 in A11-RNA 18mer and not an unforeseen artefact of the higher substrate and enzyme concentrations, a no-adenine (0A-RNA 18mer ) counterpart to A11-RNA 18mer harboring an A11G substitution was tested as a substrate for SaTrmK under the same conditions as A11-RNA 18mer , and no activity was detected (Fig. 7B). Again, orthogonal evidence for methylation of A11-RNA 18mer was obtained when A11-RNA 18mer was hydrolyzed to nucleoside 5ʹ-monophosphates by nuclease P1 following the methylation reaction and subsequently subjected to LC-MS analysis. Ions were detected with the exact m/z (362.086) expected for N 1methyl-AMP. However, no such signal was detected in a control sample where SaTrmK was omitted from the methylation reaction. A small peak rising slightly above the noise level was detected in the control with the same retention time as the presumed N 1 -methyl-AMP peak from the reaction sample, but with an m/z = 364.125 that does not correspond to N 1 -methyl-AMP (Fig. 7C).

Covalent inhibition of SaTrmK by plumbagin
In order to identify potential inhibitors of SaTrmK, in-silico screening of compounds based on molecular docking was carried out against the SaTrmK:SAM binary complex structure. This complex was chosen to increase the stringency of the screening against compounds that would compete with SAM for the cofactor-binding site, in an attempt to discover potentially novel druggable pockets in SaTrmK while increasing selectivity against the myriad other SAM-dependent methyltransferases in humans. The top hits from the screening were tested as inhibitors of SaTrmK, however, all but one, plumbagin (5-hydroxy-2-methyl-1,4-naphtoquinone), significantly interfered with the activity assay itself and were discarded. Plumbagin docked to a pocket adjacent to the SAMbinding site, making nonpolar contacts with Ile20 and Leu30 and polar interactions with Asp22 and Asp26 (Fig. 8A). Plumbagin (5 μM) led to 93% inhibition of SaTrmK-catalyzed methylation of tRNA Leu (Fig. 8B). In the absence of SaTrmK, plumbagin had a negligible effect (6% decrease) on luminescence generated by the addition of SAH to the assay (Fig. S14).
Plumbagin is a Michael acceptor and can undergo conjugate addition with amino and thiolate groups on its C3 position (27,28), and in the docking model, the C3 is 3.1 Å away from the thiol group of Cys92 (Fig. 8A). Thus, the hypothesis was considered that plumbagin inhibits SaTrmK via covalent attachment to Cys92 to form a Michael adduct. Incubation of SaTrmK with either the vehicle (1% DMSO) or with plumbagin followed by determination of the protein intact mass by ESI-MS yielded molecular mass of 25,564.9 and 25,751.1, respectively, indicating that upon incubation with plumbagin, SaTrmK mass increased by 186.2 (Fig. 8C). To identify the specific site of the modification, the samples were digested with trypsin and Glu-C and subjected to LC-MS/MS analysis. Only one peptide, spanning residues Asp84 -Lys101 (NH 2 -DVIDNITICGMGGPLIAK-CO 2 H) showed a difference in mass between vehicle-treated and plumbagin-treated samples (Fig. 8D), detected as [M+2H] 2+ = 923.4972 and [M+2H] 2+ = 1016.5033, respectively (Met94 is oxidized in both samples), which resulted in a mass difference of 186.0122; de novo sequencing showed the peptides have the same amino acid backbone but differ in mass by 186.0122 at Cys92 as indicated by the shift in the y-ion fragmentation series at y 10 (Fig. 8D). These results confirm that plumbagin inhibits SaTrmK via covalent attachment to Cys92, but the mass difference after the covalent modification is 2 mass units lower than the 188 expected for a Michael adduct (Fig. S15A).

Discussion
The high-resolution structures of SaTrmK presented here along with the functional characterization of the enzyme provide a starting point for understanding its catalytic mechanism and for designing specific inhibitors. Based on the crystal structures, SAM binding does not lead to significant conformational changes in the enzyme. Nonetheless, the different rotamer adopted by Asp26 upon SAM binding might point to a role for this residue as a general base to abstract a proton from the 6-NH 2 group of tRNA A22, thus increasing the nucleophilicity of N1. Supporting a role in catalysis, Asp26 is highly conserved among TrmK orthologues; however, in B. subtilis TrmK, replacement of the equivalent residue Asp29 for alanine had a detrimental but not completely impairing effect on catalysis (9). Moreover, similar reactions where adenine N1 acts as a nucleophile have been proposed to proceed without general base catalysis (29), likely taking advantage of the transient negative charge on N1 due to a natural resonance structure of adenine (30).
The ability of SaTrmK to bind SAM or SAH in the absence of tRNA, along with the cofactor binding site being located in a cleft in the N-terminal domain with the methyl group of SAM to be transferred pointing outwards, leads to the hypothesis the kinetic mechanism is ordered with SAM binding first to the enzyme and SAH departing last, since binding of the tRNA first would be expected to block access to the cofactor binding pocket. The K D measured here for the SaTrmK:SAH complex is in agreement with that reported for B. subtilis TrmK binding to SAH (1.7 μM) (9), whereas the K D for dissociation of the SaTrmK:SAM complex closely matches those reported for SAM binding to Sulfolobus acidocaldarius m 1 A9-tRNA methyltransferase (33 μM) (31) and to S. aureus rRNA methyltransferase OrfX (52 μM) (32). The 40-fold lower affinity of SaTrmK for SAM than for SAH is intriguing, and its structural basis is still elusive, given the remarkably similar interactions of both molecules with the active site. A 49-fold lower affinity for SAM as compared to SAH was also found with the human RNA methyltransferase methyltransferase-like 3, with K D of 0.04 μM for SAH and 1.97 μM for SAM (33).
MD simulations point to the C-terminal domain as the most conformationally flexible region of SaTrmK, in agreement with what was proposed for B. subtilis TrmK (9). In the archaeal class-I methyltransferase Trm5, an analogous, though not homologous, C-terminal domain is involved in tRNA binding away from the methylation site (8), and in B. subtilis TrmK, partial deletion of the C-terminal domain generated protein that could bind SAH with unaltered affinity but showed no catalytic activity (9). In SaTrmK, SAM and SAH binding increases the amplitude of specific motions of this domain as compared with the apoenzyme, suggesting that cofactor binding-modulated dynamics of the C-terminal domain might be involved in tRNA binding or m 1 A22-tRNA release.
In S. aureus, the m 1 A22 modification has been detected in vivo on several tRNAs (34), suggesting the presence of A22, not the identity of the tRNA itself, as the key factor in substrate selectivity by SaTrmK. This is in agreement with data for B. subtilis TrmK, where a broad substrate specificity was reported though the presence of G13 was also a requirement along with A22 (9). However, in contrast to the B. subtilis ortholog, which requires the full-length tRNA for activity, SaTrmK is able to methylate small RNA hairpins provided they harbor an adenine at certain positions. The relevance in vivo, if any, of this finding is yet unknown, but it is in line with reports for other tRNA methyltransferases which have been shown to accept small RNA hairpins as substrates in vitro, for instance Escherichia coli m 5 U54-tRNA methyltransferase (TrmA) (35) and m 1 G-tRNA methyltransferase (36).
Finally, a novel binding site was identified on SaTrmK by molecular docking, and the potential ligand plumbagin was shown to be a covalent inhibitor of the enzyme. The mechanism for covalent adduct formation is unclear. While an initial Michael addition is the most appealing hypothesis given the structure of plumbagin and its position relative to Cys92 as predicted by the docking model, extensive LC-ESI-MS/MS analysis of the SaTrmK-plumbagin adduct yields a mass difference of 186 instead of 188 as expected for a Michael adduct. One possibility is oxidation of a putative initial Michael adduct (Fig. S15B), which would result in the observed mass difference of 186. Michael adducts of plumbagin have been reported to undergo oxidation via an epoxide intermediate involving C2 and C3, for instance in the biosynthesis and organic synthesis of zeylanone (37). Again, the oxidation mechanism the plumbagin binding site on SaTrmK could accommodate is elusive. All that can be stated based on the experimental evidence is that incubation of SaTrmK with plumbagin leads to an inactivated enzyme via covalent modification of Cys92.
Elucidation of the exact mechanism for covalent modification is beyond the scope of this work, since plumbagin is not an attractive SaTrmK inhibitor per se in terms of drug development due to its lack of target selectivity, in light of its disrupting the function of several human proteins (38,39).
The key relevance of the inhibition of SaTrmK by plumbagin is the discovery of a cryptic pocket on the enzyme where a cysteine lies that is amenable to covalent modification. Cryptic pockets are potential binding sites on a protein that are not apparent from the structure of the apoprotein and usually did not evolve to bind a natural ligand, revealing themselves only when a compound binds to it (40). The plumbagin-binding site of SaTrmK is not where the cofactor binds, and judging by a docking model of tRNAbound B. subtilis TrmK (9), it is not involved in tRNA binding either. This increases the possibility that SaTrmK inhibition targeting this site will not be competitively overcome once the substrates accumulate. Furthermore, the demonstrated feasibility of covalently accessing Cys92 bodes well for increased potency and sustained target engagement of future inhibitors (41). One caveat is that Cys92 is not extensively conserved across TrmK orthologs (9), bringing about the possibility that a single mutation at position 92 could eliminate covalent inhibition without impairing catalytic efficiency. This scenario is mitigated, however, by the strict conservation of Asp22 and As26 and high conservation of the hydrophobic residues lining the pocket (9), offering opportunities for the design of alternative noncovalent inhibitors of SaTrmK that are specific, potent, and resilient.

Materials
Nucleoside 5ʹ-triphosphates, MgCl 2 , dithiothreitol (DTT), glycerol, lysozyme, DNAse I, kanamycin, spermidine, plumbagin, sinefungin, SAM, and SAH were purchased from Sigma-Aldrich. EDTA-free cOmplete Protease Inhibitor Cocktail tablets were from Roche. Isopropyl β-D-1thiogalactopyranoside, Hepes, and NaCl were purchased from Formedium, and N 1 -methyladenoside 5ʹ-phosphate (N 1 -methyl-AMP) was from Jena Bioscience. MTase-Glo Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega. All other chemicals were purchased from readily available commercial sources and were used without further purification. Tobacco etch virus protease (TEVP) was produced as previously described (42). The plasmid encoding the mutant T7 RNA polymerase Δ172-173 , which does not incorporate nontemplated nucleotides to the 3ʹ-end of RNA, was a kind gift from Dr John Perona of Portland State University, and the His-tagged enzyme was purified as previously described (43).

Cloning and expression of the gene encoding SaTrmK
The DNA encoding SaTrmK with a TEVP-cleavable N-terminal His-tag, purchased as a g-block (IDT Integrated DNA Technologies), was amplified by the PCR using the forward primer 5ʹ-GGAATTCCATATGCACCATCATC ATCACCAC-3ʹ and the reverse primer 5ʹ-CCCAAGCTTT CACAGCACACGCTCAATTA-3ʹ. The g-block was codonoptimized for expression in E. coli. The amplified DNA fragment was digested with NdeI and HindIII restriction enzymes and ligated into NdeI/HindIII-linearized pJexpress411 vector. The resulting plasmid was sequenced (Eurofins Genomics) to confirm insertion of the gene and that no mutation was introduced. The pJexpress411-SaTrmK construct was transformed into E. coli BL21(DE3) competent cells. The transformed cells were grown in 1 L of lysogeny broth containing 50 μg mL −1 kanamycin at 37 C to an optical density at 600 nm of 0.6. The culture was then equilibrated to 16 C, and expression was induced with 1 mM isopropyl β-D-1thiogalactopyranoside. Cells were allowed to grow for an additional 20 h, harvested by centrifugation at 6774g for 15 min, and stored at −20 C.

Purification of SaTrmK
All purification procedures were carried out at 4 C, and chromatographic steps employed an AKTA Start FPLC system (GE Lifesciences). Cells were allowed to thaw on ice for 20 min before being resuspended in buffer A (50 mM Hepes, 10 mM imidazole, 300 mM NaCl, pH 8.0) containing 0.2 mg mL −1 lysozyme, 0.05 mg mL −1 DNAse I, and half a tablet of EDTA-free cOmplete Protease Inhibitor Cocktail, disrupted in a high-pressure cell disruptor (Constant Systems), and centrifuged at 48,000g for 30 min to remove cell debris. The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 ml column (GE Healthcare) preequilibrated with buffer A. The column was washed with 10 column volumes of buffer A, and the adsorbed proteins were eluted with 20 column volumes of a linear gradient of 0% to 80% buffer B (50 mM Hepes, 300 mM imidazole, 300 mM NaCl, pH 8.0). Fractions containing the desired protein were pooled and dialyzed twice against 2 L of buffer C (20 mM Hepes, 150 mM NaCl, 2 mM DTT, 10% glycerol (v/v), pH 7.5). After dialysis, the protein was mixed with TEVP at a ratio of 1.3 mg of TEVP to 10 mg of SaTrmK. The protein was further dialyzed twice against 2 L of buffer C, then once against 2 L of buffer A. Samples were filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 ml column (GE Healthcare) pre-equilibrated with buffer A. The flow through was collected and analyzed by SDS-PAGE (NuPAGE Bis-Tris 4-12% Precast gels, ThermoFisher Scientific), concentrated using 10,000 molecular weight cut-off (MWCO) ultrafiltration membranes (Millipore), dialyzed twice against 2 L of 20 mM Hepes pH 8.0, aliquoted, and stored at −80 C. The concentration of SaTrmK was determined spectrophotometrically (NanoDrop) at 280 nm using the theoretical extinction coefficient (ε 280 ) of 15,930 M −1 cm −1 (Expasy) (44). The molecular mass of the protein, which contains an N-terminal glycine residue left following TEVP cleavage, was determined by ESI/TOF-MS.

SaTrmK oligomeric state determination
Size-exclusion chromatography was carried out at 20 C on a Superdex 200 10/300 Gl column pre-equilibrated with 20 mM Hepes pH 7.5, using an AKTA Purifier FPLC system (GE Healthcare). Samples (1 ml) were loaded onto the column at 1 mg/ml. Vitamin B12 (1350 Da), horse myoglobin (17,000 Da), chicken ovalbumin (44,000 Da), bovine γ-globulin (15,8000 Da), and bovine thyroglobulin (670,000 Da) (Bio-Rad) were used as MW standards. The logarithm of the MW of the standards were plotted against the ratios of the respective elution volumes (v e ) to the void volume (v 0 ). The points were then fitted to a linear regression, and the values of slope and intercept were used to determine the molecular weight of SaTrmK in solution.

Crystallization of SaTrmK
All crystals were grown at 20 C using SaTrmK (10 mg mL −1 ) in 20 mM Hepes pH 8.0. Sitting drops were set up by mixing 150 nl of protein with 150 nl of in-house and commercial stochastic screens. Diffracting crystals were obtained with 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, and 17% PEG 10,000. For SAM and SAH cocrystallization, the same condition was used, but SaTrmK was incubated with either SAM or SAH (1 mM) for 1 h prior to sitting drops being set up.
X-ray diffraction data collection and processing X-ray diffraction data were collected on beamline I04 at Diamond Light Source, UK, and processed and scaled using the automated processing pipeline with Xia2 (45) using either DIALS (46) or XDS (47). The structures were solved by molecular replacement using PhaserMR (48) using PDB entry 3KR9 (7) as the search model for the SaTrmK apoenzyme structure, which in turn was used as the search model for the SAH-bound and SAM-bound structures. Structures were refined using cycles of model building with COOT (49) and refinement with Refmac (50) and Phenix (51). Where clear electron density was observed, ligands (SAM, SAH, as well as glycerol, and citrate for the apoenzyme) were inserted in COOT using the existing coordinates and dictionaries. The structures were validated using MolProbity (52). The Rmerge was calculated with Equation 1, where I i (h) is the i th intensity measurement of a unique reflection h, and CI (h)D is the mean of all symmetry-related or replicate measurements of I (h).

MD simulations
MD simulations were carried out for SaTrmK apoenzyme and the SAM-and SAH-bound binary complexes using the AMBER99SB force field as implemented in GROMACS (53) using the respective crystal structures as starting points. To prepare the system for simulations, each structure was placed in a cubic box at 8 Å from the box boundary and solvated with TIP3P water model (54) then neutralized with potassium ions. Principal component analysis was carried out with gmx covar and gmx anaeig as implemented in GROMACS. Motional correlation studies used Wordom (55). Electrostatics were calculated using the Particle-Mesh Ewald sums method (56) with a real space cut-off of 10 Å, using order of 4 and a relative tolerance between long-and short-range energies of 10 −5 . Short range interactions were evaluated using a neighbour list of 10 Å, and the Lennard-Jones interactions, and the real space electrostatic interactions were truncated at 9 Å. The temperature was maintained at 300 K using Vrescale; hydrogen bonds were constrained using the LINCS algorithm. Energy minimization was carried out to reach a maximum force of no more than 10 kJ/mol using steepest descent algorithm. Mulliken charges of SAM and SAH were calculated for structures optimized by density-functional theory at B3LYP/6-31G(d) as implemented in GAMESS (57). The topology of SAM and SAH was defined with ACPYPE. Data reported are the mean of two independent simulations. Each structure was simulated twice for 500 ns, totaling 3 μs of simulation time. Electrostatic potential surfaces were calculated with the PDB2PQR pipeline (58) and snapshots taken every 125 ns of simulation.

In silico screening of SaTrmK-binding compounds
Simple chemical scaffolds such as benzene, pyridine, purine, and indole were used to explore the thermodynamic fit to SaTrmK:SAM complex via in silico molecular docking to ensemble conformations of SaTrmK:SAM generated by normal mode analysis. Docking studies were conducted using an in-house proprietary 7D-Grid technology that applies a geometric search algorithm to generate ligand conformations in the protein structure, which are then placed in a grid composed of quantum polarized probes to calculate the binding energies. Energy maps were computed using the fragment molecular orbital method (59). The scaffold that produced the best docked conformation was used as a template in a 3D-structure search against an in-house phytochemical database. The phytochemical database was composed of derivatives of azadirachtin from the neem tree, curcumin from the turmeric plant, cucurbitacin from squash, chlorogenic acids, and naphthoquinones from various plants.

In vitro transcription
DNA encoding for S. aureus tRNA Leu , A22C-tRNA Leu , A22G-tRNA Leu , A22U-tRNA Leu , or for small (18mer) RNA hairpins (RNA 18mer , A10G-RNA 18mer , A11U-RNA 18mer , U5C/ A14G-RNA 18mer , A11-RNA 18mer , and 0A-RNA 18mer ), all containing a T7 promoter sequence at the 5ʹ-end, were amplified using primers (IDT) listed in Table S2. PCR products were used as templates for in vitro transcription reactions. All in vitro transcriptions were carried out under RNAse-free conditions. Solutions were prepared in diethyl pyrocarbonate (DEPC)-treated water. To set up a 1-mL transcription reaction, the following components were mixed in in-vitro transcription buffer (40 mM Tris-HCl pH 8.0, 22 mM MgCl 2 , 0.2 mM spermidine, 20 mM DTT, and 0.1 μl Triton X-100) prior to incubation at 37 C for 4 h: 20 μg DNA template, 5 mM ATP, 5 mM CTP, 5 mM UTP, 6 mM GTP, 7.8 μM T7 RNA polymerase. The reaction was spun down to remove pyrophosphate. RQ1 RNAse-free DNAse (Promega) was added to the supernatant, followed by incubation at 37 C for at least 2 h. A phenol/chloroform extraction was carried out followed by an ethanol precipitation using 80 μl of 500 mM EDTA, 100 μl of 250 mM NaCl, and 9 ml of 100% ethanol. The RNA was left to precipitate at −80 C for at least 1 h and then centrifuged at 6774g for 1 h. The pellet was washed with 70% ethanol, dried, and resuspended in storage buffer (10 mM Mops pH 6.0 and 1 mM EDTA). RNAs were visualized in Novex TBE-Urea 15% Precast gels (ThermoFisher Scientific). Prior to use in any experiment, RNAs were desalted in a Bio-Rad Micro Bio-Spin column, dialyzed in Slide-A-Lyzer cassettes (Thermo Fisher Scientific) (2000 MWCO for RNA 18mer ; 10,000 MWCO for tRNA) against 200 ml of diethyl pyrocarbonate-treated water, unfolded by heating at 95 C for 10 min, and refolded by slowly cooling down at room temperature for 1 h. RNA concentration was determined spectrophotometrically at 260 nm. Secondary structure predictions for RNA 18mer were performed with RNAstructure (60).

SaTrmK activity assay
A Tecan Infinite Lumi plate reader was utilized for all SaTrmK activity measurements. The MTase-Glo Methyltransferase Assay Kit (Promega) was used to monitor the methyltransferase activity of SaTrmK. This discontinuous assay converts the reaction product SAH to ATP, which is used in a luciferase reaction, and luminescence is detected. Luminescence is correlated to SAH concentration using an SAH standard curve (0-2 μM SAH) determined under the same conditions as the activity assay; 16 nM was the lowest SAH concentration reliably detected under these conditions. All assays were performed at 20 C. Unless otherwise stated, a typical reaction mixture (20 μl) contained 100 mM Hepes pH 7.5, 3 mM MgCl 2 , 50 nM SaTrmK, 0.5 μM tRNA, or RNA 18mer , and 0.5 μM SAM. For the dependence of SAH formation on SaTrmK concentration, 0 to 150 nM enzyme was used. Inhibition by sinefungin was determined in the presence of 250 nM SaTrmK, 2 μM tRNA, and 2 μM SAM in presence or absence of 10 μM sinefungin. For the effect of sinefungin on the assay, 2 μM SAH replaced SAM, and SaTrmK was omitted. Inhibition by plumbagin was determined in the presence of 250 nM SaTrmK, 2 μM tRNA, 2 μM SAM, 1% DMSO in presence or absence of 5 μM plumbagin. For the effect of plumbagin on the assay, 2 μM SAH replaced SAM, and SaTrmK was omitted. For reactions with A11-RNA 18mer and 0A-RNA 18mer as substrates, 1 μM SaTrmK, 20 μM RNA, and 20 μM SAM were used. All reactions were incubated for 20 min, after which 5 μl MTase-Glo reagent was added, and the reaction was incubated for 30 min. Finally, 25 μl of the MTase-Glo detection solution was added, and the reaction incubated for an additional 30 min prior to luminescence counting. Control experiments lacked SaTrmK, and their signal was subtracted from the corresponding reaction signal. In the instances where the effect of inhibitor on the assay itself was tested, where SaTrmK was omitted, controls lacked SAH, and their signal was subtracted from the corresponding reaction signal with SAH. All measurements were performed at least in duplicate.

Detection of N 1 -methyl-AMP by LC-MS
Reactions (50 μl) for methylation of tRNA were carried out in 100 mM Hepes pH 7.5, 3 mM MgCl 2 , with 20 μM SaTrmK, 50 μM SAM, and 50 μM either tRNA Leu or A22C-tRNA Leu and incubated at 20 C for 2.5 h. The reaction (50 μl) for methylation of A11-RNA 18mer was carried out in 100 mM Hepes pH 7.5, 3 mM MgCl 2 , with 50 μM SaTrmK, 150 μM SAM and 150 μM A11-RNA 18mer , incubated at 20 C for 1 h, after which another 50 μM SaTrmK was added, and the reaction was incubated at 20 C for 1.5 h. For all experiments, controls lacking SaTrmK were performed under the same conditions. To each sample, 0.1 volumes of 3 M sodium acetate and 3 volumes of ethanol were added. Samples were incubated overnight at −80 C and subsequently centrifuged at 11,400g for 10 min. The pellet was dissolved in 44 μl of water, to which 5 μl of 500 mM sodium acetate pH 5.5 and 1 μl of nuclease P1 (100 U) were added, and the reaction was incubated at room temperature for 10 min, followed by addition of [ 15 N 5 ]AMP at 10 μM as an internal standard, and methanol at a final concentration of 80%. Samples were incubated at −80 C for 15 min and centrifuged at 11,400g for 10 min. The supernatant was collected, dried under N 2 and reconstituted with 50 μl of water. Commercial N 1 -methyl-AMP (10 μM) was treated in the same manner starting with the nuclease P1 reaction and used as a standard. LC-MS was performed using a Waters ACQUITY UPLC system coupled to a Xevo G2-XS QToF mass spectrometer equipped with an ESI source. The autosampler was maintained at 4 C throughout. Samples (10 μl) were loaded onto an Atlantis Premier BEH C 18 AX column (2.1 × 100 mm, 1.7 μm) (Waters) at 40 C and separated in (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile as mobile phase in the following sequence: 0 to 1 min 99% A and 1% B, 1 to 9 min linear gradient from 99% A and 1% B to 1% A and 99% B, 9 to 11 min 1% A and 99% B, 11 to 15 min 99% A and 1% B at a flow rate of 400 μl min −1 . Ion counts of the eluents were detected with a capillary voltage EDITORS' PICK: Staphylococcus aureus m 1 A22-tRNA methyltransferase of 2.5 kV in positive ion mode. The source and desolvation gas temperatures of the mass spectrometer were set at 120 C and 500 C, respectively. The cone gas flow was set to 50 L/h, while the desolvation gas flow was set at 800 L/h. An MS E scan was performed between 50 and 700 m/z. A lockspray signal was measured, and a mass correction was applied by collecting every 10 s, averaging three scans of 1 s each, using Leucine Enkephalin as a correction factor for mass accuracy.
SaTrmK thermal denaturation by DSF DSF measurements (λ ex = 490 nm; λ em = 610 nm) were performed in 96-well plates on a Stratagene Mx3005p instrument. Thermal denaturation assays (50 μl) of 7 μM SaTrmK were carried out in 500 mM Hepes pH 7.5 in the absence and in the presence of either 200 μM SAM, 10 μM SAH, or 0 to 640 μM citrate. Sypro Orange (5×) (Invitrogen) was added to all wells. Thermal denaturation curves were recorded over a temperature range of 25-93 C with 1 C min −1 increments. Control curves without the enzyme were subtracted from curves containing the enzyme. All measurements were carried out in triplicate. Data were fitted to Equation 2 (61), where F U is fraction unfolded, T is the temperature in C, T m is the melting temperature, c is the slope of the transition region, and LL and UL are folded and unfolded baselines, respectively.
Equilibrium binding by ITC ITC measurements were carried out at 20 C in a MicroCal PEAQ-ITC calorimeter (Malvern Instruments). SaTrmK and ligands (either SAM or SAH) were solubilized in 100 mM Hepes pH 7.5. An initial injection of 0.4 μl was performed, followed by 18 successive injections of 2 μl of ligand (either 1 mM SAM or 209 μM SAH) into 300 μl of either 40 μM SaTrmK (for SAM binding) or 35 μM SaTrmK (for SAH binding) with 150-s interval between successive injections and a reference power of 10 μcal s −1 . The heat of dilution for each experiment was measured by titrating SAM or SAH into 300 μl assay buffer and subtracted from the corresponding binding curve. All measurements were performed in duplicate. Binding curves were fitted to a single-site binding model as implemented in the PEAQ-ITC analysis software (Malvern Instruments). All values reported represent the mean ± standard error. One of the injections of SAM yielded an outlier, likely due to an air bubble, and was excluded from the fit.

SaTrmK intact mass and peptide analysis in the presence of plumbagin
SaTrmK (20 μM) was incubated in 100 mM Hepes pH 7.5, 3 mM MgCl 2 , 1% DMSO in the presence and absence of 30 μM plumbagin at 20 C for 20 min. Samples were diluted to obtain 1 μM protein in eluent A (95% water, 5% acetronitrile, 1% formic acid), from which 10 μl were loaded onto a Waters MassPREP desalting cartridge (2.1 mm × 10 mm) and analyzed by LC-MS using a Waters ACQUITY UPLC coupled to a Xevo G2 TOF mass spectrometer with Masslynx software. A linear gradient from 95% A and 5% B (5% water, 95% acetronitrile, 1% formic acid) to 5% A and 95% B over 6 min, before returning to 95% A and 5% B, was performed at flow rate of 200 μl min −1 . MS data were collected from 500 to 2500 m/z. The charged ion series was deconvoluted to 0.1-mass unit resolution using MaxEnt1 deconvolution algorithm using a full width at half maximum of 0.4 m/z. For proteolytic digestion analysis, SaTrmK was incubated with and without plumbagin under the same conditions described above. Samples were diluted to obtain 10 μM protein in 10 mM ammonium bicarbonate. To each 10-μL sample, 0.1 μg of trypsin was added and incubated at 30 C for 8 h, followed by addition of 0.1 μg of Glu-C and a further incubation for 8 h. Reactions were acidified and analyzed by LC-MS/MS on an Eksigent 2D ultra nanoLC coupled to a Sciex 5600+ mass spectrometer set up in trap-elute format. The sample was loaded onto a Thermoscientific 2-cm PepMap trap column and washed with 0.05% trifluoracetic acid for 5 min at 5 μl min −1 ; the trap was then switched in-line to the Thermo Scientific PepMap analytical column (75 μm × 15 cm), and a linear gradient of 0.1% formic acid to 0.1% formic acid and acetonitrile over 1 h applied. The eluting peptides were sprayed directly into the mass spectrometer. For discovery proteomics, the mass spectrometer was operated in data-dependent mode scanning MS from 400 to 1200 m/z, and the top 10 most intense peptides with charge states between 2+ and 5+ were selected for CID fragmentation with MS/MS data collected from 95 to 1800 m/z. The raw data were analyzed in Mascot 2.6 (Matrix Science) using the msconvert data extraction script (62). Data were searched against an in-house database of proteins (BMS_211,025. Fasta of 6003 protein sequences constructed in October 2021) to which the SaTrmK sequence was added using trypsin/Glu-C combined as digestion enzyme with error tolerant search settings to include any possible modification on the initial search. Subsequent searches used three missed cleavages and mass tolerances of 20 ppm on the peptides and 0.1 mass units on the fragmentation. No fixed modifications we included. Variable modifications included oxidation of methionine along with a bespoke plumbagin modification of +186 on cysteine. For confirmation of the peptide modification, the mass spectrometer was set to carry out MS scan on 400 to 1200 m/z and dedicated product ion scan fragmentation on Supporting information-This article contains supporting information.
Dr Pamela Sweeney was a Postdoctoral Fellow at the University of St Andrews, investigating the mechanism of S. aureus m 1 A22-tRNA methyltransferase, a promising target for antibiotic discovery against MRSA infections. Pamela and her collaborators identified a cryptic site on the enzyme which can be targeted by covalent inhibitors currently under development in the da Silva lab. Pamela is now a scientist with Crescendo Biologics, a biotech company in Cambridge.