Odor Characteristics of Novel Non-Canonical Terpenes

Several non-canonical, methylated terpenes have been described as products of genetically modified Escherichia coli recently, and the aroma properties of 28 odor-active methylated derivatives of prenol, isoprenol, bornane, camphene, carene, citronellol, fenchol, geraniol, limonene, linalool, terpineol, and farnesol were characterized for the first time in the current study. Twelve methylated monoterpenes exhibited a particularly intense and pleasant odor and were therefore chosen for the determination of their respective odor thresholds (OTs) in comparison to their non-methylated equivalents. In addition to the determination of OTs based on the literature value for the internal standard, (2E)-decenal, the threshold values of the compounds with individually determined OTs of the participants were calculated. This enabled a more precise identification of the OTs. Among the non-canonical terpenes, the lowest OTs in the air were found for 2-methyllinalool (flowery, 1.8 ng L−1), 2-methyl-α-fenchol (moldy, 3.6 ng L−1), 2-methylgeraniol (flowery, 5.4 ng L−1), 2-methylcitronellol (citrus-like, 7.2 ng L−1), and 4-methylgeraniol (citrus-like, 16 ng L−1). The derivatives of geraniol, linalool, and citronellol showed very pleasant odor impressions, which could make them interesting for use as flavoring agents in the flavor and fragrance industry.


Introduction
Isoprenoids are flavor compounds, which are known for their great structural diversity and their intense odor impressions. Most isoprenoids are formed from the C 5 -prenyl pyrophosphate precursors isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). The repeated appearance of isoprene units in terpene structures was enunciated as the isoprene rule [1]. Completed isoprenoid structures contain one or more isoprene units and differ in the occurrence of double bonds, carbonyl, carboxyl, keto, and hydroxyl groups. Aliphatic structures are named terpenes, whereas structures with functional groups are called terpenoids. Both terpene and terpenoid structures have been detected as secondary metabolites in plants, animals, and microorganisms [2]. Especially, short-chain terpenoids are relevant as aroma compounds, including hemi-(C 5 ), mono-(C 10 ), and sesquiterpenoids (C 15 ). A common example for hemiterpenoids is prenol, which occurs, e.g., in hop or ylang-ylang flowers [3]. Monoterpenoids and monoterpenes include highly odor-active compounds such as linalool with a citrus-and lavender-like scent, thymol with a thyme-like flavor, and limonene with a fresh, orange-like odor of the (R)-enantiomer and a pine-like flavor of the (S)-enantiomer. Farnesol and (S)-nerolidol are examples of sesquiterpenoids that are associated with a flowery scent [2].
Furthermore, Drummond et al. investigated the S-adenosyl methionine (SAM)-dependent IPP methyltransferase from Streptomyces monomycini and transferred the responsible genes in E. coli [10]. This enabled the formation of the methylated precursors (E)-, (Z)-4-methyl-IPP, 4-methyl-DMAPP, 4,4-dimethyl-IPP, and 4,4-dimethyl-DMAPP, which were released in the form of C6 and C7 alcohols. Some of these methylated precursors were accepted by a native E. coli farnesyl pyrophosphate (FPP) synthase, and the corresponding C11, C12, C16, and C17 compounds were formed. Examples of terpene alcohols identified in the mentioned study include (Z)-4-methylisoprenol 13, (E)-4-methylisoprenol 14, (E)-and (Z)-4-methylprenol 15 and 16, 4,4-dimethylprenol 17, 4,4-dimethylisoprenol 18, 4-methylgeraniol 19, 8-methylgeraniol 20, and 4-methylfarnesol 21 ( Figure 3). The biotechnological production using E. coli enabled the generation of a wide range of novel compounds, which have not been analyzed regarding their flavor properties so far. Due to their similarity to potent odor-active terpenes, they exhibited interesting flavor characteristics. Odor perception depends on the volatility of the compounds and the molecule geometry, which determines the interaction of the odotopes with the corresponding olfactory receptor proteins. Individual perceptions may differ between panelists, and the odor threshold (OT) values are not predictable so far by computational simulation [11,12]. Furthermore, fragrance impressions typically differ between the enantiomers. For instance, the mean OT of (+)-nootkatone is approximately 800-fold higher compared to that of its (-)-enantiomer (0.6-1.0 µg L −1 and 400-800 µg L −1 in water) [13]. The odor impression of (S)carvone reminds one of caraway, whereas (R)-carvone has a minty odor [14]. The methylation of aroma compounds may also lead to aroma changes and different aroma thresholds. For example, ethyl vanillin smells vanilla-like but has an OT that is four times lower than that of vanillin [15]. 2-Nonanone has a fresh, sweetish, green, and weedy flavor, whereas 2-decanone is perceived as orange, peach-like, floral, and fatty [16,17].
The determination of OTs in water (OTw) is often performed according to Czerny et al., where the component is diluted in water and evaluated in descending concentrations in a triangle test in comparison to blanks that do not contain the aroma compound [18]. Teranishi et al. used the air to water partition coefficient to calculate the corresponding OT in air (OTair). According to their theory, the OT in air is proportional to the threshold in water, only depending on the relative portions of the flavor compound in the air and dissolved in water [19]. Ullrich and Grosch established a method to determine the OT in air using gas chromatography-olfactometry (GC-O) and an internal standard (IS) [20]. The standard needs to be pure, chromatographically separated from the target compound, and must have a known OT in the air. In recent studies (2E)-decenal became the most commonly used IS [21][22][23][24]. Odor perception depends on the volatility of the compounds and the molecule geometry, which determines the interaction of the odotopes with the corresponding olfactory receptor proteins. Individual perceptions may differ between panelists, and the odor threshold (OT) values are not predictable so far by computational simulation [11,12]. Furthermore, fragrance impressions typically differ between the enantiomers. For instance, the mean OT of (+)-nootkatone is approximately 800-fold higher compared to that of its (-)-enantiomer (0.6-1.0 µg L −1 and 400-800 µg L −1 in water) [13]. The odor impression of (S)-carvone reminds one of caraway, whereas (R)-carvone has a minty odor [14]. The methylation of aroma compounds may also lead to aroma changes and different aroma thresholds. For example, ethyl vanillin smells vanilla-like but has an OT that is four times lower than that of vanillin [15]. 2-Nonanone has a fresh, sweetish, green, and weedy flavor, whereas 2-decanone is perceived as orange, peach-like, floral, and fatty [16,17].
The determination of OTs in water (OT w ) is often performed according to Czerny et al., where the component is diluted in water and evaluated in descending concentrations in a triangle test in comparison to blanks that do not contain the aroma compound [18]. Teranishi et al. used the air to water partition coefficient to calculate the corresponding OT in air (OT air ). According to their theory, the OT in air is proportional to the threshold in water, only depending on the relative portions of the flavor compound in the air and dissolved in water [19]. Ullrich and Grosch established a method to determine the OT in air using gas chromatography-olfactometry (GC-O) and an internal standard (IS) [20]. The standard needs to be pure, chromatographically separated from the target compound, and must have a known OT in the air. In recent studies (2E)-decenal became the most commonly used IS [21][22][23][24].
In this study, 28 methylated hemi-, sesqui-, and diterpenes were analyzed to determine whether they are odor-active. Especially interesting compounds were investigated by means of GC-O to determine the OT air of the methylated compounds in direct comparison to those of their non-methylated analogs. To investigate whether the published OT air of the IS (2E)-decenal is representative of the participants, the OT in water was determined for every participant and used to calculate the individual OTs in the air.

Odor Description of Methylated Hemi-, Mono-, and Sesquiterpenes
The odor impressions of methylated hemi-, mono-, and sesquiterpenes (Table 2) and of their analogous non-methylated compounds (Supplementary Materials Table S2) were described independently by 15 participants. All methylated compounds except for 6-methylfarnesol (line 6) were described with the same attributes by at least three participants. Only seven participants noted a weak odor impression for 6-methylfarnesol, whereas the others did not smell anything. The comparison of the methylated prenol derivatives with prenol and methylated isoprenol derivatives with isoprenol indicated that the position of the methyl group had an influence on the respective odor quality (Tables 2 and S2). Table 2. Odor descriptions of pure methylated hemi-, mono-, and sesquiterpenes, which were given by at least three participants, with the number of mentions in parentheses (n = 15).

Odor Thresholds in Air
The OT air of the methylated compounds were determined in comparison to their non-methylated counterparts, which were commercially available. To each terpene mixture, (2E)-decenal was added as the internal standard. The D-values of the internal standard and the other compounds slightly differed between participants. The D-value is defined according to the literature as the dilution factor in which the compound can be smelled in the lowest concentration [20] (Supplementary Materials Table S3). Odor descriptions of the participants during GC-O were similar to the descriptions of the dilutions of the standards in propandiol (Tables 2 and S4). The OT air were determined with the help of the literature value of (2E)-decenal of 2.7 ng L −1 and, additionally, with the individually determined OT air of each participant as described above (Figure 4) [17]. The thresholds of (2E)-decenal determined for the participants were higher than those reported in the literature. The OT air values of 1-methylcamphene, 4-methyl-3-carene, 2-methylcitronellol, 2-methylgeraniol, 4-methylgeraniol, and 2-methyllimonene were comparable to those of their non-methylated equivalents. 8-Methylgeraniol, 2-methylnerol, and 2-methyllinalool showed higher OTs than the respective corresponding C 10 compounds.

Odor Thresholds in Air
The OTair of the methylated compounds were determined in comparison to their nonmethylated counterparts, which were commercially available. To each terpene mixture, (2E)-decenal was added as the internal standard. The D-values of the internal standard and the other compounds slightly differed between participants. The D-value is defined according to the literature as the dilution factor in which the compound can be smelled in the lowest concentration [20] (Supplementary Materials Table S3). Odor descriptions of the participants during GC-O were similar to the descriptions of the dilutions of the standards in propandiol (Tables 2 and S4). The OTair were determined with the help of the literature value of (2E)-decenal of 2.7 ng L −1 and, additionally, with the individually determined OTair of each participant as described above (Figure 4) [17]. The thresholds of (2E)decenal determined for the participants were higher than those reported in the literature. The OTair values of 1-methylcamphene, 4-methyl-3-carene, 2-methylcitronellol, 2-methylgeraniol, 4-methylgeraniol, and 2-methyllimonene were comparable to those of their nonmethylated equivalents. 8-Methylgeraniol, 2-methylnerol, and 2-methyllinalool showed higher OTs than the respective corresponding C10 compounds.

Discussion
The odors of 28 methylated terpenes were described. All of the studied C 6 -, C 7 -, and C 11 compounds were perceived as aroma-active, but the methylated farnesol derivatives had only weak odors. The odor impression of a substance depends on different factors.
Besides the air-to-water partition coefficient, the individual associations to known odor impressions and the interactions with the receptors in the olfactory epithelium are essential for the individual perception of the substances [25]. The descriptions of the odor characteristics varied among the participants, but the panel agreed on a set of attributes that represented the respective essential characteristics.
Methylated aliphatic monoterpenes and methylated monoterpenoids showed the most intense odor impressions. They have molecular masses close to those of other highly odor-active compounds and high structural similarity to monoterpenes, which are welldescripted aroma compounds. Furthermore, the odor of a compound depends on the distribution between hydrophilic and lipophilic structure elements [26,27]. This matches the observation that the terpenoids had marginally lower OTs than the aliphatic terpenes.
The human nose has approximately 430 different types of receptors [25]. The odor impression of a compound is the result of their interaction with different odotopes, which creates a pattern of signals, associated with a familiar odor. Thereby, the odor impression is dependent on the individual receptors of the nose, the association based on memories of the flavor, the health status, the age, and on other individual factors. Therefore, odor descriptions may differ between persons, and the individual thresholds can vary significantly [25,28].
Some of the non-canonical terpenes imparted especially interesting odor impressions. Methylcitronellol exhibited a very pleasant, intense aroma, which combined a citrus odor with intense flowery flavors. It may thus represent an interesting fragrance ingredient for cosmetics. Furthermore, the odor of methylcarene was described as fruity, sweetish, and coniferous forest-like, whereas (S)-carene has a resinous odor, resembling a coniferous forest. The influence of the position of the methyl group and of the stereochemistry was shown for geraniol and nerol. Geraniol with a double bound in the (E)-configuration has a citrus-like and flowery odor, whereas its isomer nerol, with the double bound in the (Z)-configuration, has a resinous, citrus-like, and flowery odor. The methylation of both compounds led to changes in the odor descriptions. The methylation of geraniol in position 8 led to a more resinous odor, the methylation in position 4 to a lemon-like odor, and the methylation in position 2 did not change the odor impression. All nerol derivatives showed a citrus-like, fruity odor but had slightly different odors. While nerol was described as resinous, flowery, citrus, and terpene-like, 2-methylnerol was sweetish, flowery, fresh, citrus, and orange-like. In contrast, 4-methylnerol was ascribed as green, fruity, flowery, and citrus-like. Furthermore, the flavor of (R/S)-methyllinalool stood out as very pleasant, similar to linalool but with notes of lemon and bergamot. Several synthetic terpenoids were developed to meet the rising need for flavoring agents. Some have intensive and highly pleasant aroma properties. For instance, the derivatives of ionone Iso E Super Plus ® (CAS 140194-26-9) and (-)-georgywood ® (CAS 828933-31-9) have odor thresholds of only few pg L −1 and are widely used in the cosmetics industry [29,30]. According to their odor properties, the novel geraniol and linalool derivatives could also be interesting flavoring agents, especially considering the fact that linalool and geraniol are two of the most often used flavor compounds in cosmetics, deodorants, and showering agents [31,32].
OTs air of several monoterpenes have been determined in previous studies according to the method of Ullrich et al. [20]. Nevertheless, it is necessary to determine both odor descriptions and OTs by the same panelists to directly compare methylated and nonmethylated equivalents. Overall, similar odor descriptions and OTs values as those reported in the literature have been determined in this study for monoterpenoids, but deviations were found for some compounds (Table 3). (R)-linalool 0.036 [35] 0.098 ± 0.064 n.d. = not determined.
In particular, the OTs of the two enantiomers of citronellol were 20-fold higher than those reported by Schoenauer and Schieberle [34] but were comparable to the values determined by Elsharif and Buettner [33,34]. The individual human perception of odors varies greatly in terms of quality, threshold, pleasantness, and intensity, as it depends, e.g., on the health status, genetics, age, gender, and aroma compound [36]. Nevertheless, the panel was sensitive for all of the analyzed compounds.
The comparison of the OTs of non-canonical terpenes with those of their canonical equivalents revealed some significant differences. While similar OTs were determined for methylated carene, nerol, limonene, and 2-methylgeraniol, the thresholds of methyllinalool and 4-methylgeraniol were higher than those of their non-methylated counterparts. Surprisingly, the OT of methylcitronellol, which had a similar odor impression as citronellol, was lower than that of citronellol.
According to Teranishi et al., the OT in the air is directly proportional to the OT W , only depending on the air-to-water partition coefficient [19]. Two of the panelists could detect the odor of (2E)-decenal during GC-O in all dilutions up to 1:64 and one participant up to 1:128. The panelist who perceived the odor up to 1:128 dilution also had the lowest OT W (0.5 ± 0.1 µg L −1 ). The OT in water of the two participants who detected the odor until a dilution of 1:64 were 0.9 ± 0.3 and 1.8 ± 0.6 µg L −1 in water. The thresholds determined in water of all participants differed from the literature threshold of (2E)-decenal (0.3 µg L −1 ) [19]. The lowest concentration at which the participants could detect the odor during GC-O was proportional to the individual OT in water. Using the threshold from the literature leads to a less precise determination of the OTs by GC-O as the same value was taken for all participants even if their OTs differed and they perceived the odor until different dilution steps. Furthermore, the sensitivity of the human nose can also be different for different compounds [36]. Accordingly, the determination of individual OTs of the IS is as important as the individual determination of the thresholds of the new compounds.
For some compounds, the OT adopted for the internal standard did not significantly influence the calculations of the OTs. However, there was a strong influence observed for some of the evaluated compounds. For instance, the standard deviations calculated for 2-methylcitronellol, citronellol, 8-methylgeraniol, 4-methylnerol, and geraniol were multiple-fold smaller, with the individually determined OTs compared to those using the fixed literature OT. On the other hand, for α-fenchol, (R)-limonene, and 2-methyllinalool, the standard deviations were higher with the individually determined thresholds. Overall, the method proposed here is more precise, and the calculated thresholds of the analyzed compounds were higher when the individually determined OTs of (2E)-decenal were used for calculation. The individual OTs of an IS may be used to determine OTs for additional ISs, which could be more similar to the analyzed compounds, as suggested by Ullrich and Grosch [20]. If the air-to-water partition coefficient or the Henry constant are known, every substance could be used to calculate the threshold in the air.

Sensory Analysis
Fifteen participants (eight women, seven men, 23-34 years) described odors of the pure compounds, dissolved in 1,2-propandiol, freely. Therefore, 1 µL or 0.95 mg were dissolved in 200 µL of 1,2-propandiol, and 4 µL of the solutions were placed on a filter paper strip and marked with a three-digit code. The intensity of each odor impression was evaluated from 0 (no odor) to 5 (very intense odor).

Sensory Analysis
Fifteen participants (eight women, seven men, 23-34 years) described odors of the pure compounds, dissolved in 1,2-propandiol, freely. Therefore, 1 µL or 0.95 mg were dissolved in 200 µL of 1,2-propandiol, and 4 µL of the solutions were placed on a filter paper strip and marked with a three-digit code. The intensity of each odor impression was evaluated from 0 (no odor) to 5 (very intense odor).

Gas Chromatographic Analysis
The retention indices of the analytes on a polar column and their respective mass spectra were measured with a gas chromatography-mass spectrometry (GC-MS) sys-tem. An Agilent 7890A GC, together with an Agilent 7000B MS triple Quad (Agilent Technologies, Santa Clara, CA, USA) equipped with a VF-WAXms column (30 m, ID 250 µm, film thickness 0.25 µm; Agilent Technologies), were used. Helium 5.0 (Nipon Gasses GmbH, Hürth, Germany) was used as the carrier gas with a constant flow rate of 1.56 mL min −1 . The gas flow was split 1:1 between the MS and the ODP port (ODP 3, GER-STEL GmbH & Co. KG, Mülheim a.d., Ruhr, Germany). One microliter of the sample solution was injected in a splitless liner at 250 • C. The oven was heated to 40 • C (3 min)/5 • C min −1 /240 • C (12 min). The mass spectrometer was equipped with an electron ionization source (230 • C, 70 eV) and operated in scan mode (m/z 33-300).
The retention indices on the non-polar DB-5ms column (30 m-320 µm-0.25 µm) were determined by means of a gas chromatography-flame ionization detector system (GC-FID) with a 7890 A GC (Agilent technologies). Measurements were performed as indicated above, except for the following parameters: the carrier gas was hydrogen 5.0 (Nipon Gasses GmbH) with a flow rate of 2 mL min −1 , and the oven was heated with the same ramp to 320 • C (12 min). The FID was heated at 250 • C. Retention indices (RI) were calculated according to van den Dool and Kraatz [37]. Chiral analyses were performed using a GC-FID 6890A (Agilent Technologies) equipped with a Hydrodex β-6-TBDM column (25 m-250 µm, Macherey Nagel GmbH & Co. KG, Düren, Germany). One microliter was injected in a splitless liner, which was heated to 250 • C. The GC oven was heated at 80 • C (0 min)/2 • C min −1 to 150 • C/20 • C min −1 to 250 • C (5 min). The pressure was constant at 0.8 bar, with nitrogen as the carrier gas.
The ratios of (E) and (Z) isomers were calculated with Formula (1).

Odor Thresholds of the Internal Standard (2E)-Decenal
The odor threshold of the internal standard (2E)-decenal in water (OT W,IS ) was determined in pure water, as described by Hammer et al. [21]. The initial concentration was 38 µg L −1 , and the solution was diluted 1:2 (v/v) nine times. The tests were carried out in triplicate by each of the three participants, who also performed the GC-O analyses.
The corresponding individual odor threshold in the air of the internal standard (OT air,IS ) was then calculated for each participant according to Teranishi et al. with the help of the previously determined odor threshold in water (OT W,IS ) and the air-to-water partition coefficient K W with Formula (5) [19].
OT air,IS = OT W,IS · K W = OT W,IS · c air c W

Odor Thresholds in Air
The analyses were done according to Hammer et al., with adapted oven temperature ramps [21]. The compounds were dissolved in methanol, and the concentrations were chosen individually according to their respective aroma potency. The compounds were analyzed in four mixtures (Table 4). Table 4. Composition of the four mixtures used for the GC-O analysis for determination of the odor thresholds in the air. to 240 • C (4 min). The mixtures were successively diluted 1:2 (v/v) with methanol. The determination of the OT air by GC-O was done by one man and two women, which were 24-29 years old. Samples were analyzed in a random order, and each participant noted the odor individually.

Compounds
The OT air of the analyzed compound X (OT air,X ) was calculated with the OT air of the IS (2E)-decenal (OT air,IS ), the initial concentration of the analyzed compound c x , the D-value of the IS D IS , the initial concentration of the IS (c IS ), and the D-values of the analyzed compound D x and of the IS D IS with Formula (6).
OT air,x = OT air,IS ·c x ·D IS c IS ·D x (6)

Conclusions
This study characterized 28 novel methylated terpenes regarding their odor and their chemical characteristics, including mass spectra and retention indices on two columns of different polarities. Thirteen of the evaluated non-canonical terpenes showed intense aroma impressions, and the OTs were determined for the first time in comparison to those of eleven reference terpenes. Individual determination of the OTs of the IS enabled us to determine the thresholds more precisely and to expand the options for an internal standard.

Patents
Parts of this study are included in a European Patent "The use of non-canonical terpenes or terpenoids as aroma chemicals", Sommer, S., Fraatz, M.A., Zorn, H. (19 May 2022, EP 22174377.6).

Conflicts of Interest:
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results.
Sample Availability: Samples of the compounds are not available from the authors.