Apatite phosphate doped by cobalt as hight efficient catalyst of multi-component synthesis of therapeutic spiropyrimidine compound

Spiropyrimidines vanquish a significant situation in the field of heterocyclic chemistry, they are broadly utilized as an antibacterial, an inhibitor of multidrug opposition, or an antiplatelet and antithrombotic drug. Phosphate, the principal Moroccan mineral wealth occupies a vital spot in the economic sector and its valuation is a goal continually looked for. Among the conceivable outcomes of valorization, its utilization in heterogeneous catalysis which thought about an exceptionally encouraging new way. In this context, the focus on the reaction of the synthesis of spiropyrimidine, catalyzed by natural phosphate (NP) and by fluoroapatite (Fap), are used alone or doped by cobalt. These phosphate catalysts were characterized by XRD, IR and SEM, while the synthesized spiropyrimidine was identified by IR, GC–MS and NMR. The obtained yield with fluorapatite doped by cobalt (Co/Fap), at room temperature in ethanol is very high and shows a high impact performance induced by a synergistic effect compared to that of NP alone. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s13659-022-00359-8.


Introduction
In recent years, the chemical industry has experienced a growing need for environmentally friendly chemical processes based on sustainable technologies. This requires a shift from the concept of increasing the economic gains Page 2 of 6 Rhihil et al. Natural Products and Bioprospecting (2022) 12:35 of chemical processes to the concept of eliminating waste at the source and the atom economy. Accordingly, much attention has recently been given to the development of clean techniques for the synthesis of finer products. Many organic reactions had been catalyst by natural or synthetic's phosphate such as the Claisen-Schmidt condensation [1], known for the synthesis of chalcones utilized in the treatment of different diseases such as the fight against leukemia cancer. The Suzuki-Miyaura carbon coupling for the synthesis of biphenyls, is used in the therapy of disorders caused by atherosclerosis [2]. The notable Knoevenagel condensation for the preparation of benzylidene malononitrile (BMN) rumored to be powerful inhibitors of tyrosine kinase [3] and the synthesis of α-hydroxyphosphonates by KF/NP [4] and the transposition of α-hydroxyphosphonates by K/NP [5]. These items have organic exercises and drug applications, which had been catalyzed by natural phosphate (NP) [6] or fluoroapatite (Fap) and fluoroapatite alone or doped with metals, showing extremely very high performance.
The spiropyrimidines compounds are the most encountered heterocycles in medicinal chemistry with different antibacterial, antiviral, antitumor and anti-inflammatory applications. This synthesis is carried out by catalysis, a method used in organic chemistry transformations. It has known a very considerable development in the field of scientific research, more particularly heterogeneous catalysis, due to its ease of use, the reuse of the catalyst and its recovery.
The synthesis of biologically active heterocyclic products, by the catalysis of the multicomponent reaction is a powerful strategy to obtain spiropyrimidines in the presence of a solid catalyst by fluoroapatite doped with cobalt (Co/Fap).
N-substituted hexahydropyrimidines are spermidinenitroimidazole drugs for the treatment of lung carcinoma A549 [7]. N-(4-Aminobutyl) hexahydropyrimidine and N-(3-aminopropyl) hexahydropyrimidine have been shown to compete with spermidine for uptake by L1210 cells, as well as the conditions for optimal growth in suspension culture [8]. They form structural units in trypanothione reductase inhibitory ligands for the regulation of oxidative stress in parasitic cells [9], due to their significant biological activity, hexahydropyrimidines have received attention in recent years.

Preparation of the fluorapatite doped by cobalt (Co/ Fap) catalyst
Fluorapatite doped by cobalt (Co/FAP) was elaborated by co-precipitation, following a similar process as fluorapatite (Fap) [10]. The Ca 8 Co 2 (PO 4 ) 6 F 2 is prepared under the temperature of 80 °C, flack 7.93 g of (NH 4 ) 2 HPO 4 , 70 mL of NH 4 (OH), 1 g of NH 4 F in 250 mL ultra-pure water, and adding on the mixture 18.89 g of Ca(NO 3 ) 2 and 5.82 g of Co(NO 3 ) 2 6H 2 O dissolved in 150 mL of ultra-pure water. The mixture was heating for 3 h at pH 9, the formed powder is washed with ultra-pure water and dried for 24 h at 150 °C and then calcined at 600 °C for 2 h.

Characterization of the catalyst
The diffractogram of fluorapatite doped by cobalt Ca (10-2) Co 2 (PO 4 ) 6 F 2 is identified as that of the fluorapatite [7]. The outcomes are given underneath in the accompanying Fig. 1.
The X-ray diffraction pattern of the Co/Fap synthesis was highly selective where cobalt is incorporated in the structure. There is an absence of impurities such as calcium fluoride or phosphate, and cobalt or calcium oxide. Indeed, the X-ray diffraction pattern of the calcined Co/ Fap showed the appearance of a single solid phase which is similar to the fluorapatite (Ca 10 (PO 4 ) 6 F 2 ) [11].
The analysis of fluorapatite doped by cobalt using Scanning Electron Microscope (SEM) shows that the material agglomerates with a size under 2 µm (Fig. 2).
The IR spectrum of Co/Fap shows bands at 556 and 607 cm −1 connected with the PO 4 3− moiety as well as new groups at 2850 and 2926 cm −1 that didn't exist in the Co/Fap IR range. This implies that cobalt is very much embedded in the fluorapatite structure. Table 1 shows the IR retention groups of Co/Fap.

Procedure for the synthesis of spiropyrimidine and catalysts effect
Multi-component reactions allow getting objective molecules from more than two reagents. One of them is the multicomponent reaction for the synthesis of spiropyrimidines (Scheme 1). In this work, various tests were performed to examine the effectiveness of Co/Fap for catalysis of the multicomponent reaction of spiropyrimidine, while following the procedure (Fig. 3): In 25 mL boiling flasks the three beginning reagents: 2 mmol aniline, 1 mmol cyclohexanone and 3 mmol formaldehyde was mixed, 5 mL of ethanol as solvent had been added, the reaction blend was mixed at room temperature for 3 h. At the end of the reaction, the mixture was isolated from the catalyst by vacuum filtration, the solvent was evaporated by rotavapor, and the product item was gotten is purified by recrystallization. Several heterogeneous catalysts were tested in the multicomponent reaction of synthesis. The results are presented in Fig. 4.
In the wake of testing several solid catalysts in this reaction, including natural phosphate (NP), NP doped with lanthanum (La/NP), NP doped with cobalt (Co/ NP), fluorapatite (Fap), Fap doped with lanthanum (La/ Fap) and Fap doped with cobalt (Co/Fap), we noticed that Co/Fap gave the best yield (66%), and NP alone gave a yield of 21%, while without a catalyst, the reaction is inactive.
The speroperimidine 2,4-bis-phenyl-2,4-diazaspiro [5.5]undecane-7-one obtained was identified by gas chromatography GC-MS and characterized by IR, 1 H NMR and UV.     After the preliminary study which included the catalysts study with the same operative conduction and an amount of 20 mg, it was found that fluorapatite doped by cobalt is a good catalyst for the multicomponent reaction of spiropyrimidine synthesis.

Mass and solvent effects
The study of the mass impact of catalyst uses in the multicomponent spiropyrimidine reaction is performed using successively 20, 40, 60, 80, and 100 mg of the Co/ Fap catalyst, with following the procedure described above (Fig. 3). The results are regrouped in Fig. 5.
From the obtained results, it can be seen that 60 mg of the catalyst is the sufficient amount to obtain yield of 81%, beyond this amount, the yield drops to 74%.
The solvent is an important factor to promote contact between the components of the reaction mixture. To optimize this factor, several solvents have been used while keeping the same conditions as before. The obtained results are shown in Fig. 6.
It is noticed that ethanol gave a yield of 81% compared to acetonitrile and dichloromethane which gave a relatively low yield of 57% and 51% respectively (Fig. 6). While in the presence of water or without solvent, the reaction is practically inactive.

Kinetic study of the reaction and temperature effect
The yield of the reaction was performed at various temperatures from 25 °C to 65 °C (Fig. 7). It is noted that by increasing the temperature, the reaction yield decreases to 30% at 65 °C, while the best yield 81% is obtained at room temperature.
The reaction time was improved, while keeping the same experimental conditions, in the presence of Co/Fap. The synthesis of spiropyrimidine was performed under the same conditions as before and at room temperature of the reaction medium. The increase in the yield of the reaction was observed at 3 h of the reaction time, beyond that, a stabilization of the yields was reached (Fig. 8).  Table 2.
The comparison of the yields of spiropyrimidine synthesis, under the reaction operating conditions, in the presence of different catalysts, in a heterogeneous (category A) and homogeneous (category B) media, reported in the literature, with that obtained with fluorapatite doped by cobalt, shows that our catalyst can be considered among the most efficient catalysts, next to Dy(III)/Chitosan [16], taking into consideration the amount of catalyst used, the reaction temperature and the required advantages of the type of reaction media, in other words catalysis in a heterogeneous solid/liquid medium. So, Co/Fap allows to have a yield of 81%, at room temperature, a reaction time not exceeding 3 h and with only 50 mg of the catalyst.
The obtained results are very interesting and open another way to explore this phosphate catalyst in the synthesis of other compounds biologically active, and respecting the environment and the principles of green chemistry.

Conclusion
Given the importance of spiropyrimidine products and their biological activities as well as its applications in the medical field in chemotherapy as anti-cancer in the fight against lung carcinoma A549, against L1210 cells and leukemia, as well as for the regulation of oxidative stress in parasitic cells, in the context of environmental protection and clean chemical synthesis, the focused in heterogenous catalysis as one of the fundamental pillars of green chemistry, which aims at atoms saving and reducing the chemical impact on the environment.
The heterogeneous catalysis by phosphates has had a great development in several chemical processes, in particular in organic synthesis, with interesting catalytic efficiency, thanks to the facility of its preparation, its great reactivity and selectivity and its abundance, offering a great advantage compared to homogeneous catalysis. NP have both acidic and/or basic properties, structure flexibility, capacity to disperse a catalytic active phase, and low cost.
The focused is on the optimization of the spiropyrimidine synthesis reaction, studying the effects of influencing parameters on the reaction yield. From the results of the tests performed, a maximum yield of 81% was obtained using ethanol as solvent, with 60 mg of catalyst Co/Fap, working at room temperature and with a reaction time of 3 h.
This work allowed us to show the effectiveness of the Co/Fap catalyst as an efficient catalyst for this type of reaction, and opens the perspective to make further studies generalize this synthesis (Additional file 1).  Aniline (S)-proline 20 mol %/DMSO (5 mL) B 25 30 53 [18]