Benzophenone Derivatives Showed Dual Anti-Inflammatory and Antiproliferative Activities by Inhibiting COX Enzymes and Promote Cyclin E Downregulation

Considering the promising antitumor effects of compounds with dual anti-inflammatory and antiproliferative activities, thus benzophenones analogs (2-7) were evaluated on in vivo antiinflammatory assay and molecular docking analysis. Those with the best molecular docking results were in vitro evaluated on cyclooxygenase (COX) enzymes and tested regarding antiproliferative activity. All derivatives displayed in vivo anti-inflammatory activity. Among them, the substances 2’-hydroxy-4’-benzoylphenyl-β-D-glucopyranoside (4), 4-hydroxy-4’-methoxybenzophenone (5) and 4’-(4’’-methoxybenzoyl)phenyl-β-D-glucopyranoside (7) showed the best values of Glide Score in COX-2 docking evaluation and 4 and 5 selectively inhibited COX-2 and COX-1 in vitro enzymatic assay, respectively. Thus, 4 and 5 were tested against breast cancer (MCF-7, MDA-MB-231, Hs578T) and non-small-cell-lung cancer (A549) cell lines. The estrogen-positive MCF-7 cell line was more responsive compared to other tested cell lines. They induced cell cycle arrest at G1/S transition in MCF-7 cell line once there was an increase in G0/G1 population with concomitant reduction of S population. The antiproliferative activity of these substances on MCF-7 was associated with their ability to inhibit cyclin E expression, a critical regulator of G1/S transition. Taken together, the data indicate that 4 and 5 have dual anti-inflammatory and antiproliferative activities and support further studies to evaluate their antitumor potential.


Introduction
Chronic inflammation is associated with different pathological processes including cancer development and cancer progression. 1,2 The constant exposure to inflammatory mediators such as arachidonic acid metabolites, cytokines, chemokines, and free radicals can contribute to uncontrolled cell proliferation, mutagenesis, angiogenesis, and activation of oncogenic pathways. 3,4 Nonsteroidal anti-inflammatory drugs (NSAIDs) have demonstrated potential in the prevention and treatment of cancer 2 and maybe incorporated in chemotherapy and radiotherapy regimens. 5 Besides cancer, other diseases are also associated with inflammation, which arouses interest in anti-inflammatory drug screening since the available NSAIDs have recognized side effects and limited efficacy in many of these cases. [6][7][8] Ketoprofen is a potent NSAID with excellent analgesic properties. It possesses a diphenylmethanone nucleus (benzophenone) and acts by inhibiting prostaglandin synthesis, as well as by inhibiting both forms of cyclooxygenase (COX-1 and COX-2). 9 It is effective and generally well-tolerated in a variety of inflammatory disorders, including osteoarthritis and rheumatoid arthritis. 10 Studies with ketoprofen have, in general, showing that it has efficacy equivalent to or greater than that of other NSAIDs. 11 Moreover, increased anti-inflammatory activity and high selectivity for COX-2 have been described for new ketoprofen derivatives. Some ketoprofenamides with a heterocycle have exhibited significant analgesic and anti-inflammatory activities when compared to the parent drug. 12 In this context, the diphenylmethanone nucleus has been shown to be a versatile pharmacophore group. [13][14][15][16] It has been reported that compounds containing carbohydrates in their structure display various pharmacological activities, including anti-inflammatory and cytotoxic effects. The presence of a saccharide moiety has been shown to be important in improving drug solubility, stability, and/or interaction with the receptor. 17 Therefore, the synthesis of benzophenone derivatives containing carbohydrate units has gained considerable interest regarding the evaluation of their potential as new anti-inflammatory drugs. 18 As mentioned before, benzophenones have potent anti-inflammatory activity, and the presence of carbohydrates in the structure may ultimately help to facilitate their interaction with the molecular target and improve their pharmacokinetic properties. [19][20][21] In addition, some glucoside derivatives have also shown anticancer activity 22,23 and antiinflammatory potential. 18 Thus, the development of new glucosides with dual anti-inflammatory and anti-cancer properties would be extremely relevant to the search for new clinically effective prototypes. Benzophenone glucosides can be synthesized using a classical method of reaction between the phenol and the base on the per-acetylglucosyl bromide, or by glucosylation of phenols through phase transfer, as an option possible with high yield. [24][25][26] In the present work, innovative glucosides benzophenones were synthesized by these two methods ( Figure 1) aiming to identify substances with dual anti-inflammatory and antiproliferative activities. Aglycone benzophenone precursors also were considered in this investigation. The glucosylated derivative 4 and the aglycone 5 displayed significant anti-inflammatory activity and selectively inhibited COX-2 and COX-1, respectively. The same substances inhibited cell proliferation in estrogenpositive breast MCF-7 cells due to their ability to modulate cyclin E expression and induce cell cycle arrest at G1/S transition.

Synthesis of peracetylglucosyl bromide 1
The α-D-glucosylbromide was synthesized previously described method 27 by the D-glucose peracetylation and further reaction of the peracetylated glucose with hydrobromide acid (Fluka, St. Louis, USA) in acetic anhydride (Proquímicos, Rio de Janeiro, Brazil). Their spectra data were coherent with literature data. 27 Synthesis of 4-hydroxy-4'-methoxybenzophenone 5 To a solution of 4,4'-dihydroxybenzophenone (9.3 mmol) in acetone (10 mL) (Synth, Diadema, SP, Brazil) was added Na 2 CO 3 (9.3 mmol) (FMaia Indústria e Comércio Ltda, BH, Brazil) and the mixture was stirred at room temperature for 20 min. After this time, iodomethane (Sigma-Aldrich, Saint Louis, MO, USA) was added (9.3 mmol) and the completion of the reaction was noted after 1 h by TLC. The acetone was evaporated, and the resulting product was partitioned into 10% NaOH (FMaia Indústria e Comércio Ltda, BH, Brazil) solution in water (m/v) and dichloromethane (Synth, Diadema, SP, Brazil). The basic layer was acidified to pH 1, extracted with dichloromethane and this new organic layer was washed with water, dried over anhydrous Na 2 SO 4 (FMaia Indústria e Comércio Ltda, BH, Brazil), and concentrated under reduced pressure. The product of interest was isolated from the crude product by column chromatography (hexane/ ethyl acetate; Synth, Diadema, SP, Brazil), with a yield of 21%. Their spectra data were coherent with literature data. 28 General procedure for the synthesis of glucosides 3 and 6 The glucosides 3 and 6 were synthesized by method A ("Reaction with glucosylbromide, phenol and base-method A" sub-section) and method B ('Reaction with phase transfer-method B" sub-section) to a comparison of yield in each method.

Reaction with glucosylbromide, phenol and base-method A 25
To a solution of peracetylglucosyl bromide (1, 1.7 mmol) in acetone (Synth, Diadema, SP, Brazil) was added 5 mL of an aqueous solution of the corresponding benzophenone (5.1 mmol) (Sigma-Aldrich, Saint Louis, MO, USA) and LiOH (4.6 mmol) (Sigma-Aldrich, Saint Louis, MO, USA) and the mixture was stirred at room temperature for 3 h, when the completion of the reaction was confirmed by TLC. The acetone was evaporated, and the resulting product was extracted with dichloromethane. The obtained organic layer was washed with HCl 10% and water until pH 7 was attained, then dried over anhydrous Na 2 SO 4 and concentrated under reduced pressure. The crude product was purified by recrystallization from isopropyl alcohol (Synth, Diadema, SP, Brazil).

Reaction with phase transfer-method B 24
The corresponding benzophenone (1 mmol) was solubilized in dichloromethane (5 mL) and to this solution was added 10% K 2 CO 3 (10 mL) (Proquímicos, Rio de Janeiro, Brazil) and the mixture was stirred at room temperature for 30 min. After this time, it was added to a reaction flask containing tetrabutylammonium bromide (0.34 mmol) (Sigma-Aldrich, Saint Louis, MO, USA) and finally, the peracetylglucosyl bromide (1.1 mmol) was solubilized in dichloromethane. The mixture was stirred at room temperature for 72 h when the end of the reaction was confirmed by TLC. The mixture was extracted with dichloromethane and the organic layer was washed with water until pH 7, dried over anhydrous Na 2 SO 4 , and concentrated under reduced pressure. The crude product was purified by recrystallization from isopropyl alcohol.
General procedure for the synthesis of glucosides 4 and 7 The procedure was performed according to de Souza et al. 29 The peracetylated glucosides (3 and 6, 0.2 mmol) were solubilized in a solution of KOH (FMaia Indúdtria e Comércio Ltda, Diadema, SP, Brazil) in MeOH (20 mL, 1 M) (Synth, Diadema, SP, Brazil) and stirred for 30 min at 0 ºC. The completion of the reaction was confirmed by TLC. The mixture was neutralized with 1 g of Amberlite ® IR120 resin. After neutralization, the resin was filtered off and washed with methanol. The collected filtrate was concentrated under reduced pressure, yielding the deacetylated glucosides derivatives 4 and 7.
Chemical characterization of the compounds 3, 4, 6 and 7 All the spectra data of IR, NMR, and mass (MS) are available in the Supplementary Information section (Figures S1-S16).

Animals
The animal experiment was approved by the Ethics Committee of the Federal University of Alfenas (506/2013). The adult male Swiss mice weighing 28-32 g, obtained from the Central Animal Facility of the Federal University of Alfenas, were housed under controlled light (12/12 h light-dark cycle) and temperature conditions (23 ± 1 ºC) with access to water and food ad libitum.

Croton oil-induced ear edema
Ear inflammation in the mouse was produced as described previously. 30,31 The assay was done using 7 animals per group of the substances or positive controls: ketoprofen and indomethacin (Sigma-Aldrich, Saint Louis, MO, USA). The substances and positive controls were administered orally 1 h before the application of 20 μL croton oil solution (Sigma-Aldrich, Saint Louis, MO, USA; 5% v/v in acetone) to the inner surface of each left ear and 20 μL of acetone to the right ear as a control. All samples, substances (aglycones 2 and 5, and the derivatives 3, 4, 6, and 7), and positive controls were administered in the same dose (0.5 mg per ear) according to established protocols. 30,32,33 Edema was measured 6 h after starting the experiment, determined as the weight difference between 6 mm plugs taken from the left and right ears. The data are expressed as mean ± standard error of the mean (SEM) and were analyzed by one-way analysis of variance (ANOVA) followed by the Newman-Keuls post-hoc test. P-values < 0.05 (P < 0.05) were considered significant.

Molecular modeling
The structures of the ligands, indomethacin and ketoprofen (standard drugs used in the pharmacological and experimental evaluations) were constructed using Maestro 9.2. 34 The software LigPrep 2.5 35 was used for the construction and preparation of the ligands involved in these studies. The crystallographic structures of cyclooxygenase 1 (COX-1) (Protein Data Bank (PDB) ID: 2OYU) and cyclooxygenase 2 (COX-2) (PDB ID: 3NT1) were obtained from the database PDB and the software Prime 3.0 36 was used for the preparation of this enzymes. The OPLS 2005 force field in the MacroModel 9.9 37 was used for optimization. Studies of molecular docking between COX-1 and COX-2 and the ligands were performed using the program Induced Fit Docking. 38 All computer programs belong to the Schrödinger suite.
Cyclooxygenase assay Substances 4, 5, and 7 were evaluated using a COX-1 and COX-2 (catalog No. 560101 Cayman Chemical, Ann Arbor, MI, USA) screening kit, according to the manufacturer's instructions and established protocol. 39 Ketoprofen was evaluated as the reference inhibitor for COX-1 and COX-2, and the vehicle was evaluated as the negative control. All substances were evaluated in the following concentrations: 0.01, 1, 10, and 100 µM in the final reaction volume. The concentration of each compound causing a 50% inhibition (IC 50 , µM) was calculated from the concentration-inhibition response curve, with data collected in triplicate. Sigma, Saint Louis, MO, USA) to obtain a stock solution, which was subsequently diluted in fresh medium at the appropriate concentrations immediately before use. Vehicle alone was used as the control and the final concentration of the solvent did not exceed 0.5% (v/v). The cell cultures were treated for 48 h with the different substances.

Cell viability assay
Cell viability was assessed in tumor and normal cells according to well-established protocols. 40,41 Cells were seeded into 96-well flat-bottom plates at a density of 5 × 10 3 cells well -1 or 1 × 10 4 cells well -1 depending on the cell line. Cell cultures were treated with substances 4 or 5 (concentration range 0-500 µM) for 48 h. Ketoprofen and cisplatin (Sigma-Aldrich, Saint Louis, MO, USA) were used as controls. All experiments were conducted in quadruplicate wells, and the data are presented as median ± standard deviation (SD) of three independent experiments. Viable cells with active metabolism can reduce resazurin (absorption peak at 600 nm) into the resorufin product (absorption peak at 570 nm). Cell viability analysis was performed previously. 42 Significant differences from the control group (DMSO) were determined using ANOVA followed by Tukey's post-hoc test.

Cell cycle analysis
Cell cycle analysis was performed according to Ferreira-Silva et al. 43 Briefly, cells were treated with 4 and 5 for 48 h in concentrations equivalent to IC 50 values. Cells were fixed with 75% ethanol (Sigma-Aldrich, Saint Louis, MO, USA) at 4 °C overnight, rinsed twice with cold phosphate-buffered saline (PBS). Afterward, cells were homogenized in dye solution (PBS containing 90 μg mL -1 propidium iodide (PI) and 3 mg mL -1 RNAase) (Sigma-Aldrich, Saint Louis, MO, USA), and deoxyribonucleic acid (DNA) was quantified 1 h after staining. The analysis was performed using a flow cytometer (Guava easyCyte 8HT, Luminex, Austin, Texas, USA). Results are presented as mean ± SD of three independent experiments. Significant differences from the control group (DMSO) were determined using ANOVA followed by Tukey's post-hoc test.

Chemistry
The glucosides of benzophenones were synthesized following classical methods, as shown in Figure 1. There are different described glycosylation methods involving the reaction of a glucosyl donor that generates an electrophilic compound, which interacts with a nucleophilic reagent to form a glycosidic linkage with the first chemical entity. 44 The development of improved methods of glycosylation is especially important and necessary due to the biological importance of these compounds. 45 In this work, two different methods for the synthesis of glucosides of benzophenones were employed (Figure 1). The starting benzophenone 4-hydroxy-4'-methoxybenzophenone 5 was synthesized by the reaction of 4,4'-dihydroxybenzophenone and iodomethane, with a yield of 21%. Initially, benzophenone glucosides were synthesized using a widely described classical method involving the nucleophilic attack of the phenoxide ion generated from the reaction between the phenol (corresponding benzophenone) and the base on the per-acetylglucosyl bromide. 25,26 The glucosides 3 and 6 were obtained in yields ranging from 14 and 25% by this method (A).
Considering these low yield values, their synthesis was repeated employing another method for the glucosylation of phenols that used tetrabutylammonium bromide as a phase transfer-method B. 24 The compounds synthesized by the phase transfer method were obtained in yields twice as high as those of the classical technique (54 and 51% yields for 3 and 6, respectively). Ester and ketone bands in 1740 and 1743 cm -1 , respectively, were observed in the IR spectra of these compounds ( Figures S1 and S9, SI section). The acetylated glucosides' 1 H NMR spectra showed singlets corresponding to acetylic hydrogens near 2 ppm; signals relative to the aromatic hydrogens were registered between 7.81-6.46 ppm (Figures S2 and S10, SI section). The carbonyls of these compounds' benzophenone rings were recorded between 200. .0 ppm, while acetylic carbonyls were registered around 170 ppm in the 13 C NMR spectra ( Figures S2 and S11, SI section).
All acetylated glucosides of benzophenones were deacetylated by stirring them in a potassium hydroxide/ methanol solution for 30 min, affording yields around 85% to the unprotected glucosides 4 and 7. 29 The IR spectra of these compounds did not show the ester bands registered for acetylated glucosides but, concomitantly, contained large bands close to 3300 cm -1 which correspond to the hydroxyl groups of deacetylated derivatives (Figures S5 and S13, SI section). As noted in the glucosides' 1 H NMR spectra, all of them were obtained as β-anomers (Figures S6 and S14, SI section). This was certified by the coupling constant of 7.3 Hz for the anomeric hydrogens, which was registered close to 5 ppm (Figures S6 and S14, SI section). Finally, only one carbonyl signal (benzophenone ring) was registered at 193-198 ppm in the 13 C NMR spectra ( Figures S7 and S15, SI section).

Anti-inflammatory results
The glucosylated derivatives 4, 6 and 7 effectively inhibited ear edema statistically similar to the starting benzophenones and the control drugs, all with p < 0.001 compared to the negative control (vehicle). The acetylated derivative 3 also inhibited ear edema, but with p < 0.01 compared to negative control. All the compounds were evaluated at the same dose (0.5 mg per ear) to assess antiinflammatory activity, which significantly inhibited edema according to one-way ANOVA followed by Newman-Keuls test (Figure 2). The values for edema inhibition for the free aglycones 2 and 5 were 55 and 53%, respectively, while the glucosides displayed 51, 67, and 73%, for 4, 6 and 7, respectively-statistically similar to the values found for the NSAIDs used as positive controls (62% of inhibition for indomethacin and ketoprofen).

Molecular docking analyses
In order to check the affinity profile of the synthesized compounds with cyclooxygenases 1 and 2 (COX-1, COX-2), a docking study was carried out. 46,47 The results of analysis of all compounds (2-7) showed Glide score (GScore) values between -14.245 and -8.677 kcal mol -1 (Tables S1 and S2, SI section) and they suggest that these high affinities can lead to the inhibition of such isoenzymes.
The results (Tables S1 and S2) revealed that compounds 4, 5, and 7 presented better GScore values in docking molecular of COX-2 enzyme than the other compounds. Hydrogen bond interactions of each compound's greatest affinity conformations could be visualized in Figures 3 and 4.
It was observed that the glucosides presented better values of GScore than the non-glucosylated derivatives, which showed a smaller number of hydrophobic interactions (good van der Waals) and a low profile of liposolubility. Considering the glucosylated compounds, the non-acetylated substances (derivatives 4 and 7) presented better values of affinity compared to the acetylated derivatives (3 and 6). The acetylated derivative 3 did not present GScore results in molecular docking studies that could be related to its molecular volume, leading to a steric hindrance in relation to the size of the COX-2 active site and corroborating the results of the edema bioassay lower percentage of edema inhibition (35%) compared to the other evaluated compounds.
The glucoside 4 presented the best value of Gscore for both isoenzymes (COX-1 = -15.230 kcal mol -1 and COX-2 = -14.245 kcal mol -1 ), with higher values of affinity than those presented by the standards drugs, indomethacin and ketoprofen. Although compound 4 showed no selectivity between the two isoenzymes, the results of the molecular docking study are in accordance with the edema inhibition results (51%). The glucosylated compound 7 presented the second-best GScore value for the two enzymes (COX-1 = -14.169 kcal mol -1 and COX-2 = -13.504 kcal mol -1 ), and the aglycone 5 showed the third-best GScore value for COX-2 (-11.330 kcal mol -1 ). Thus, compounds 4, 5, and 7 with better docking results were selected for in vitro evaluation on COX enzymes. The results of molecular docking showed that the other substances, as compound 6, also have the potential to inhibit the COX-1 or COX-2 enzyme, thus, they may be evaluated in future studies.

In vitro COX inhibition
In this context, after anti-inflammatory assay and molecular docking, the glucosides 4 and 7 and the aglycone 5 were evaluated about the inhibition of the isoenzymes COX-1 and COX-2 (Table 1). Derivative 4 showed selective inhibition for COX-2 at 4 µM, while 7 was inactive for both isoforms. The presence of the 4-methoxy Figure 2. Effect of benzophenone glucosides on croton oil-induced ear edema in mice. The substances, ketoprofen (Keto) and indomethacin (Indo) (0.5 mg per ear) were orally administered before the application of croton oil solution in acetone (20 μL per ear). Positive and negative control groups were significantly different. One-way analysis of variance (ANOVA) followed by Newman-Keuls test was employed. Data represent the mean ± SEM of 7 animals (**p < 0.01; ***p < 0.001). group and the absence of a 2-hydroxy group in substance 7, compared to substance 4, seem to have been critical to abolish its activity on COX-2. Substance 5 selectively and moderately inhibited COX-1 at 67.25 µM.
Additionally, it was possible to determine that the edema inhibition caused by substance 7 was not a result of COX-1 or -2's direct inhibition, as demonstrated by the enzymatic findings. They may be due to its molecular volume leading to a possible steric hindrance to allow access to the enzyme active sites. These results suggest that these compounds could have an innovative mechanism of action that deserves further investigation.

Antiproliferative activity
The antiproliferative potential of the substances 4 and 5 was evaluated against tumor cell lines derived from human breast cancer (MCF-7, Hs578T, and MDA-MB-231) and non-small cell lung cancer (A549). Cell viability was assessed after 48 h of treatment and IC 50 values were determined ( Table 2). The data showed that MCF-7 cells were the most responsive for both substance 4 and 5 compared to other evaluated tumor cells. The IC 50 values found for these substances on MCF-7 were lower than those found for ketoprofen; but higher compared to cisplatin, a powerful cytotoxic agent. In addition, substances 4 and 5 displayed low cytotoxic activity on PBMC, which were included in this study to evaluate the effects of these substances on normal cells.
Based on previous findings, MCF-7 was selected for further investigation to verify whether the reduction in cell viability promoted by substances 4 and 5 could be associated with their ability to inhibit cell proliferation; however, it is important to note that substances 4 and 5 had an interesting effect on Hs578T and MDA-MB-231, respectively. These cell lines are representative of triplenegative breast cancer, a subtype of breast cancer treated preferentially with cytotoxic chemotherapy due to the absence of therapeutic targets. The drug arsenal for triplenegative breast cancer treatment is extremely limited and the patients frequently develop resistance to currently available drugs. 48,49 In the next step, cell cycle analysis was performed to investigate a possible interference of the substances 4 and 5 on MCF-7 cell cycle progression after 48 h of the treatment. Cell cultures were photographed immediately before the cell cycle analysis to evidence morphological features of MCF-7 cells. The morphological pattern of cells treated with 4 was similar to observed in control cultures, however, the samples treated with 5 exhibited rounded cells or shrinkage cells (Figure 5a) indicating a cytotoxic effect of this compound on MCF-7 cells. Indeed, flow cytometry data showed that sub-G1 populations (dead cells) were 7.0-fold and 4.6-fold higher, respectively, in cultures treated with substances 5 and 4 in relation to control groups. In addition, it was demonstrated that substances 4 and 5 altered the MCF-7 cell cycle progression. There  was an increase of the G1 population with a concomitant reduction in the S population in treated samples compared to control groups (Figure 5b and Table 3). These findings indicate that substances 4 and 5 inhibited the cell cycle at G1/S transition. Further reduction of cyclin E expression was evidenced by immunoblot in samples treated with substances 4 and 5 (Figure 5c). Cell cycle progression is highly regulated by sequential activation and deactivation of specific cyclin-CDK (cyclin-dependent kinase) complexes, in which the cyclins are regulatory subunits. G1 progression and G1/S transition are regulated by cyclin D-CDK4/6 and cyclin E-CDK2 complexes, respectively. 50 G1/S transition is a critical step for cell proliferation. 51 Deregulated expression of cell cycle regulators is frequently observed in cancer cells, and overexpression of cyclins D and E have been reported in different subtypes of breast cancer. [52][53][54][55] Moreover, overexpression of cyclin E has been correlated with resistance to hormonal therapy and poor patient outcomes. 56,57 Cell cycle arrest at G1/S transition was reported in MCF-7 cells when exemestane, an aromatase inhibitor, was used in combination with NSAID. 58 Thus, substances 4 and 5 may be valuable prototypes for breast cancer therapy due to their ability to inhibit cyclin E expression and, in turn, provoke cell cycle arrest at G1/S transition. Taken together, the data show that compounds 4 and 5 have anti-inflammatory and antiproliferative activity on Ketoprofen was used as a typical anti-inflammatory drug. b Cisplatin was used as a typical antineoplastic drug. SD: standard deviation; ND: not determined because cell viability was not enough reduced to determine IC 50 values; NP: not performed.

Conclusions
Glucosyl analogs of ketoprofen were synthesized and evaluated for the first time as new anti-inflammatory and antiproliferative agents. Molecular modeling studies showed that glucoside 4 has a higher affinity for COX-1 and COX-2 than ketoprofen. In in vitro studies, the glucoside 4 selectively inhibited COX-2 while its aglycone, the benzophenone 5, selectively inhibited the COX-1 isoform. In addition, we demonstrated that these two compounds have in vivo anti-inflammatory effect and inhibit cell cycle arrest at G1/S transition in MCF-7 cells. Anti-inflammatory activity was attributed to their ability to inhibit selectively COX enzymes, but other pathways which were not evaluated also can contribute. While antitumor potential, at least in part, was due to their ability to modulate cyclin E expression. Thus, this study provides strong evidence that these substances have both antiproliferative and anti-inflammatory activities, deserving further studies to evaluate their applicability as an antitumor agent for breast cancer therapy.

Supplementary Information
Supplementary file (containing the NMR, MS, and IR spectra of the synthesized compounds and table of molecular docking results) is available free of charge at https://jbcs.sbq.org.br as a PDF file.