Glycal mediated synthesis of piperidine alkaloids: fagomine, 4-epi-fagomine, 2-deoxynojirimycin, and an advanced intermediate, iminoglycal

Glucal and galactal are transformed into 2-deoxyglycolactams, which are important building blocks in the synthesis of biologically active piperidine alkaloids, fagomine and 4-epi-fagomine. In one of the strategies, reduction of 2-deoxyglycolactam-N-Boc carbonyl by lithium triethylborohydride (Super-Hydride®) has been exploited to generate lactamol whereas reduction followed by dehydration was utilized as the other strategy to functionalize the C1–C2 bond in the iminosugar substrate. The strategies provide the formal synthesis of 2-deoxynojirimycin, nojirimycin and nojirimycin B. DFT studies were carried out to determine the reason for the failure of the formation of the 2-deoxygalactonojirimycin derivative. Further, DFT studies suggest that phenyl moieties of protecting groups and lone pairs of oxygen in carbamate group plays a vital role in deciphering the conformational space of the reaction intermediates and transition-state structures through cation–π or cation–lone pair interactions. The influence of these interactions is more pronounced at low temperature when the entropy factor is small.


Introduction
Polyhydroxylated alkaloids are of considerable interest as potential therapeutic agents, they are also used as an important tool to understand biological recognition processes. 1 Hence, their synthesis and biological activity studies have assumed signicance. These occur in the species of Streptomyces, family Leguminosae, Solanaceae, and Convolvulaceae and possess therapeutic potential. 1 Nojirimycin 1 ( Fig. 1) was the rst natural polyhydroxylated piperidine alkaloid isolated from a Streptomyces ltrate in 1966 by Inouye et al. 2 1,2-Dideoxyiminosugars exemplify a small but essential class of glycosidase inhibitors. 3 One of the members of this family, fagomine 5a, was isolated from the seeds of Japanese buckwheat Fagopyrum esculentum australe Moench 4 and also from the seeds of Castanospermum australe 5 (Leguminosae). It has also been reported that 4-epi-fagomine 5b (Fig. 1) acts as a potent glycosidase inhibitor. 6 The batzellasides 6 are a novel class of Calkylated iminosugars originally isolated from Batzella sp., a marine sponge from Madagascar 7a and are active against Staphylococcus epidermidis. Their unique structural features resemble an intriguing extension of the iminosugar frameworks, hence their synthesis 7b,c has attracted the attention of researchers who practice contemporary drug discovery.

Results and discussion
Reported synthesis of fagomine and 4-epi-fagomine involves either use of carbohydrate building blocks 8,9 or noncarbohydrate precursors. 10 Although nojirimycin 1 is very active glycosidase inhibitor, 2b it has been observed that the deoxy derivatives displays a broad range of activities and are much more stable than nojirimycin 1 itself thereby attracting interest of synthetic chemists on the synthesis of deoxynojirimycin. 11 We have designed the synthesis of fagomine 5a and 4-epifagomine 5b using carbohydrate building blocks as shown in Scheme 1. Fagomine 5a and 4-epi-fagomine 5b could be synthesized by deprotection and reduction of the corresponding 2-deoxyglycolactams 7a/b. By lactamization of d-hydroxy amides 8a/b, 2-deoxyglycolactams 7a/b could be obtained. d-Hydroxy amides 8a/b could be accessed by aminolysis of 2deoxyglycolactones 9a/b which in turn could be obtained from readily available tri-O-benzyl-D-glucal 10a or tri-O-benzyl-D-galactal 10b respectively (Scheme 1). Nitrogen from ammonia is the source of the heteroatom in our planned synthesis of piperidine alkaloids.
d-Hydroxy amides 8a/b under Albright Goldmann oxidation condition i.e. Ac 2 O and DMSO at rt provided the desired d-keto amides 11a/b which without purication was carried on for the next step. The crude of the above reaction was then treated with formic acid and NaBH 3 CN, to furnish the desired 2-deoxyglycolactams 7a/b. This crucial step comprises an intramolecular reductive amination, which involved condensation of the amine with the ketone to furnish the cyclized product. Formic acid complexes with the ketone carbonyl and increases its electrophilicity to facilitate the attack of amine, leading to the formation of a new C-N bond. The iminium ion 14 formed in situ aer dehydration from the intermediate 12a/b undergoes NaBH 3 CN reduction to form the desired 2-deoxyglycolactams 7a/b (Fig. 2).
It is noteworthy here to describe the stereochemical course of the reduction of Schiff base 14. The mechanism of this step presumably involves a hydride donation by the NaBH 3 CN reagent to the acyliminium ion 14a/b, initially formed by an acid-catalyzed dehydration of the hydroxy lactam substrates 12a/b. Attack of hydride can take place from b-face as well as from the a-face leading to the formation of 2-deoxyglycolactam. 14a The 2-deoxyglycolactam adopts a stable half chair conformation. 14b Here the reduction is governed by the stereoelectronically controlled transition states. Attack of hydride from the b-face results in an unfavorable twist boat conformation 15T which then ips to a favorable half chair conformation 15 ( Fig. 2(a)). However, as 15a/b was not formed it indicates that hydride approaches from the a-face of the piperidine ring, in which hydride orbital overlaps effectively with the orbitals of the double bond which in turn are in conjugation with the lactam carbonyl. Moreover, during a-face hydride attack there is no formation of twist boat conformation transition state, instead this directly leads to the formation of 2deoxyglycolactam product in stable half chair conformation 7a/ b ( Fig. 2(b)). None of the other products being formed and the NMR of the products 7a/b supports the stereoselective reduction step.
Reduction of the carbonyl group of 2-deoxyglycolactam 7a/ b shall furnish the desired benzyl protected fagomine 13a and 4epi-fagomine 13b which was successfully achieved by carrying out the reaction in THF with slow addition of LiAlH 4 at 0°C and then to rt and nally reux for around 4 h to yield benzyl protected fagomine 13a in 49% and benzyl protected 4-epi-fagomine 13b in 41% respectively from the corresponding 2deoxyglycolactams 7a/b (Scheme 2). Finally, following the reported procedure by Shipman et al. 8a or Vankar et al. 8b benzyl deprotection can be carried out to furnish fagomine 5a and 4epi-fagomine 5b respectively in 12% and 13% overall yields from the corresponding 2-deoxyglycolactones 9a/b.
Intrigued by the amazing chemistry of 2-deoxyglycolactams 7a/b, we envisioned that 2-deoxyglycolactams 7a/b can be utilized to synthesize an advanced intermediate viz. iminoglycal 17 from which the biologically important piperidine alkaloids such as nojirimycin and its analog 2-deoxynojirimycin derivative can be readily synthesized. Iminoglycals were obtained as important reaction products 15a,b or were intermediates 15c synthesized which are utilized further for C-O 15d or C-C bond forming reactions at C-1 of the piperidine nucleus, for the synthesis of natural products like (+)-deoxoprosophylline, 15e,f (+)-fagomine, 8a (−)-1-epi-adenophorine 15g and potential immunosuppressant compounds. 15h Although many syntheses of nojirimycin are reported, 11a it is noteworthy to mention that synthesis of 2-deoxynojirimycin derivative is less known, so far we came across only two references. 7b,c Hence, we formulated a retrosynthetic plan for its synthesis from a common intermediate as shown in Scheme 3.
We visualized that N-protected iminoglycal 17 can be readily obtained in 3 steps from 2-deoxyglycolactam 7 by (i) Nprotection, (ii) reduction of the carbonyl (iii) elimination. Appropriate stereoselective dihydroxylation of 17 will furnish protected nojirimycin or galactostatin derivative which on deprotection will give the desired nojirimycin 1 and galactostatin 3. On the other hand, 2-deoxyglycolactamol 19 can be obtained by (i) N-protection and (ii) partial reduction of the carbonyl group of 2-deoxyglycolactam 7. 2-Deoxynojirimycin 18a or 2-deoxygalactonojirimycin 18b can be synthesized from 19a/b by deprotection reaction condition.
Our idea was then to partially reduce the carbonyl group of 2deoxyglycolactams 7a/b and also to subsequently bring the dehydration of the lactamol hydroxy group. To proceed for that we started with 2-deoxyglycolactams 7a/b and treated with NaBH 4 in MeOH following the reported procedure 16c carried out on the similar type of substrates (Table 2, ESI †). However, in all the cases even with 2-deoxyglycolactam-N-Boc 20a/b and also under varying conditions and solvents desired product was not formed (Table 2, ESI †).
During our literature survey in the synthesis of fagomine, 4epi-fagomine, and nojirimycin, we visualized that so far Super-Hydride or LiBHEt 3 are used mainly for three purposes (i) regioselective epoxide ring opening 10c,17 (ii) reduction of the ester to alcohol 18 and (iii) displacement of triate -OTf by hydride. 9a There are hardly any references for the use of Super-Hydride in the reduction of amide carbonyl and which is utilized in the synthesis of piperidine alkaloids. On extensive search we came across only three references using Super-Hydride in the reduction of carbonyl but it is not used on iminosugar substrate. 19 This inspired us to use Super-Hydride in our synthetic strategy of iminosugar. As we proposed in our retrosynthetic plan in partial reduction of amide carbonyl and also partial reduction followed by elimination of OH group to introduce a double bond i.e. to generate an iminoglycal 18a/b, we took advantage of Super-Hydride here to play the double role. With ne-tuning of the reaction conditions using Super-Hydride the reaction shall proceed to furnish the desired products 21a and 17a/b (Scheme 4).
(sat) solution at −76°C to rt furnished the desired 2-deoxygluconolactamol 21a in 94% yield. The 1 H NMR spectrum of 2deoxygluconolactamol 21a d H 5.64 brs represents H1 protons deshielded due to -OH and also by N atom. Reduction happened chemoselectively at d-lactam carbonyl only, as the CO group of Boc was observed intact at 156.7 ppm in 13 C NMR spectrum. However, when 2-deoxygalacatonolactam-N-Boc 20b was treated with Super-Hydride under similar conditions, desired product 2-deoxygalactonolactamol 21b could not be formed but starting material was recovered (Scheme 4). Interestingly, during our literature survey we observed that 3,4-di-epiisomer of 21a was utilized by Yoda et al. 7b,c for the synthesis of (+)-batzellaside B 6 and its C8-epimer. By known methods viz. aq. HCl, MeOH, 76°C, and then hydrogenolysis with H 2 , Pd-C (10%), AcOH can easily furnish the desired product 2-deoxynojirimycin 18a from 21a.
It was then necessary to reduce and dehydrate both in situ the 2-deoxyglycolactam-N-Boc 20 to generate iminoglycal 17a/b. This was achieved when 2-deoxyglycolactam-N-Boc 20a/b was subjected to reaction conditions 19a,c by treating with Super-Hydride, in toluene at −76°C for 30 min, and then with TFAA and base DIPEA in presence of catalytic DMAP, then raising the temperature from −76°C to rt, starting material was consumed in 12 h, with 90% 17a and 87% 17b respectively from 20a and 20b (Scheme 4). It was noteworthy that carrying out the reaction at −76°C or −70°C had no profound effect on the yields of the products, we preferred carrying the reaction at lower temperature (−76°C) to the reported reaction condition 19c at −70°C. In 1 H NMR spectrum of iminoglucal 17a, d H 7.11-6.93 (m, 1H), 5.10-4.90 (m, 1H), corresponds to vinylic protons, 13 C NMR d C 101.5 indicates the chemical shi of b-carbon of enamine which is shielded by N atom. The other enamine a-carbon signal is merged with the aromatic carbons which appear in the range 128.6-126.6 ppm. However, in this case by 13 C NMR isomeric mixture of products could be predicted possibly due to the presence of a Boc group which attains different conformation, in the plane of piperidine ring and out of the plane of the piperidine ring.
To further extensively understand the reaction step for the selective conversion of 20a to 21a (Scheme 4), we performed the Density Functional Theory (DFT) calculations for this step of reaction involving both the substrates 20a and 20b. We have come up with the following explanation for the above selective conversions. The formation of 2-deoxygalactonolactamol 21b that could not be isolated under the conditions (a) given in Scheme 4 is endergonic. However, the formation of 17b under condition (c) (ii) from 20b further suggests that 21b is in dynamic equilibrium with 20a under condition (a), as the subsequent treatment of 21b formed in situ with TFAA and base DIPEA in the presence of catalytic DMAP condition (c) (ii) gives the desired dehydrated product 17b. To further elaborate the above point, a relative DFT studies involving the substrates 20a and 20b for this particular step of the reaction was performed. The computational details are provided in the ESI. † Two different pathways corresponding to the approach of the Super-Hydride to the re-and si-faces of the prochiral substrates (20a/b) have been investigated. Both modes of approach are associated with two different conformations (C 1 and C 2 ) of reactant and product complexes and the transition state geometry (see, computational details section (ESI †) for more details). Results of the gas-phase calculations are provided in Tables 3 and 4 (see ESI †) for 20a and 20b respectively. Please refer to Fig. S1 and S2 in the ESI † for the optimized gas-phase transition state geometries involving 20a and 20b. The results of gas-phase calculations reveal that the conformation C 2 is signicantly more stable than the conformation C 1 . Therefore, the free energy prole diagrams and all the further analyses are exclusively based on conformation C 2 . Furthermore, the solvent-phase calculations were performed only on conformation C 2 . The results of the solvent phase calculations are provided in Table 5 (see ESI †) and the optimized solvent-phase transition state geometries are represented in Fig. S3 (see ESI †).
Therefore, two different pathways (each with two different conformations of reactant and product complexes and transition-state geometries) have been investigated for both the substrates, 20a and 20b ( Fig. S1 and S2 in ESI †). Results of the gas-phase calculations are provided in Tables 3 and 4 in the ESI † for 20a and 20b, respectively. Tables 3 and 4 † reveals that conformation 2 for each mode of approach for both the substrates are energetically favorable. Therefore, the free energy prole diagrams and all the further analyses for both the substrates exclusively involve conformation 2. It is to be noted that the solvent-phase calculations were performed only on conformation 2 for both the pathways for the two substrates. Fig. 3 The gas-phase free energy profile for reduction of 2-deoxygluconolactam-N-Boc 20a to 2-deoxygluconolactamol-N-Boc 21a representing approach of the Super-Hydride to: (a) re-face of 20a and (b) si-face of 20a. R s , R c , T 1 , I 1 represent infinitely separated reactants, the reactant complex, the transition state geometry, and the product complex, respectively. Hydrogen atoms that are not part of the reaction coordinate have been removed for clarity. All the values are in kcal mol −1 . Hydrogen atoms that are not part of the reaction coordinate have been removed for clarity. Color codes: blackcarbon, coral oxygen, cyanhydrogen, light greenlithium, fuchsiaboron, bluenitrogen. The dotted pink lines represent cation-p interaction and dotted maroon lines represent bond breaking/bond forming.
The results of the solvent phase calculations are provided in Table 5, ESI. † Fig. 3 illustrates the gas-phase free energy prole for the conversion of 2-deoxygluconolactam-N-Boc 20a to 2-deoxygluconolactamol-N-Boc 21a involving both the re-and si-face pathways. A very strong interaction between the Super-Hydride and 20a has been observed, as the reactant complex in the pathway shown in Fig. 3(a) (for the re-face approach) is 25.5 kcal mol −1 lower in energy than the innitely separated reactants. Besides, the reactant complex for the si-face approach is 2.5 kcal mol −1 less stable as compared to the re-face reactant complex. The subsequent barriers for the conversion to 2-deoxygluconolactamol-N-Boc 21a for the re-and si-face approaches are 15.1 and 12.5 kcal mol −1 , respectively. However, the relative energies of two transition state geometries with respect to the innitely separated reactants are almost the same. Further, the product complex for the si-face pathway was found to be 3.0 kcal mol −1 more stable than the re-face approach (Fig. 3). Notably, both the pathways lead to stable product complexes (the intermediates for the next step of the reaction) with the reaction step being exergonic. Thus, one would expect that both the pathways are feasible for 20a in the given condition, which leads to product 21a that we were able to isolate.
The solvent phase calculations (employing toluene as solvent) for 20a reveal a more distinct trend. The re face pathway is kinetically favorable with a lower (by 2.5 kcal mol −1 ) barrier as compared to the si-face pathway ( Fig. S4a and b in ESI †). The reactant complexes for both the pathways are almost of the same energy. However, the product complex obtained for the siface approach is 3.7 kcal mol −1 lower in energy than the re-face product, depicting the si-face pathway is thermodynamically favorable. Moreover, both the pathways reveal the process being exergonic with thermally stable product complexes, which could be isolated aer subsequent hydrolysis. Fig. 4 illustrates the gas-phase free energy prole for the reduction of 2-deoxygalactonolactam-N-Boc 20b by Super-Hydride. The reactant complex for the re-face pathway, in this case, is 7.8 kcal mol −1 lower in energy than the si-face reactant complex, which according to the Boltzmann distribution law substantiates for more than 99.99% of the population in this microstate. Thus, any further chemical conversion is possible only through the re-face reactant complex, and hence it rules out the si-face pathway for the reduction of the 20b using Super-Hydride. The subsequent barrier for the reduction of the 2deoxygalactolactam-N-Boc 20b (through re-face approach) is 19.6 kcal mol −1 , with the overall reaction step being endergonic by 7.8 kcal mol −1 . The solvent-phase calculations also reproduce the gas-phase ndings with slightly altered energy values ( Fig. S4c and d, ESI †). The reactant complex for the re face approach in the solvent-phase was obtained to be 4.8 kcal mol −1 lower in energy than the si face reactant complex, which accounts for this microstate being occupied by about 99.99% of the total population (as per the Boltzmann distribution law). Thus, the solvent-phase results also indicate the selective reduction through the re face of the substrate 20b. The obtained barrier for the re face approach in the solvent-phase was obtained to be 17.6 kcal mol −1 . Importantly, this barrier with respect to the innitely separated reactants is merely 0.1 kcal mol −1 . Thus, it could be deduced that the barrier is easily surmountable at the reaction temperature (−76°C). However, the product complex obtained for this pathway in the solventphase is 3.8 kcal mol −1 higher in energy than the reactant complex, implying that the reaction of 20b with Super-Hydride is endergonic by 3.8 kcal mol −1 . This suggests that the product is signicantly unstable in the case of 2-deoxygalactolactam-N-Boc, and that is why it could not be isolated experimentally.
These results corroborate our original hypothesis that, 20a a relatively stable product under the condition (a) given in Scheme 4, and hence the corresponding 2-deoxyglucolactamol-N-Boc 21a could be isolated experimentally. However, 20b forms an unstable product complex, which converts back into the reactant under condition (a) Scheme 4, and thus could not be separated under the applied experimental conditions. However, the product was not extensively unstable and the barrier height was also surmountable at the given temperature. Therefore, when the conditions for the forthcoming step (TFAA and base DIPEA, catalytic DMAP then to rt) were enforced immediately, a successful conversion of 21b to 17b was achieved. Thereby provides the reason why 2-deoxyglucolactamol-N-Boc 21a could be isolated, whereas 2-deoxygalactolactamol-N-Boc 21b could not be.
By synthesizing the iminoglycal 17a/b we have functionalized the C-1 and C-2 position of iminosugar, which can grant access to the synthesis of various other biologically active Fig. 4 The gas-phase free energy profile for reduction of 2-deoxygalactonolactam-N-Boc 20b to 2-deoxygalactonolactamol-N-Boc 21b representing approach of the Super-Hydride to: (a) re-face of 20b and (b) si-face of 20b. R s , R c , T 1 , I 1 represent infinitely separated reactants, the reactant complex, the transition state geometry, and the product complex, respectively. Hydrogen atoms that are not part of the reaction coordinate have been removed for clarity. All the values are in kcal mol −1 . Hydrogen atoms that are not part of the reaction coordinate have been removed for clarity. Color codes: blackcarbon, coraloxygen, cyanhydrogen, light greenlithium, fuchsia boron, bluenitrogen. The dotted pink lines represent cation-p interaction and dotted maroon lines represent bond breaking/bond forming. piperidine alkaloids. As per our retrosynthetic plan dihydroxylation of iminoglycal can procure nojirimycin 1 and nojirimycin B 4 (mannojirimycin) (Scheme 4). Following the very well-established condition for dihydroxylation 20a with AD-mix a and AD-mix b (commercial reagent) in t-butyl alcohol-H 2 O (1 : 1) 0°C for 4 days, resulted in complete recovery of starting material. Strong chelating ligands and using methane sulfonamide are known to accelerate the dihydroxylation reaction which prompted us to try this reaction condition by using strong chelating ligands (DHQ) 2 AQN and (DHQD) 2 AQN. 20b,21 To our surmise, on treating iminoglucal 17a with (DHQ) 2 AQN (5 mol%), with K 3 Fe(CN) 6 as oxidant and K 2 CO 3 as a base, K 2 OsO 2 (OH) 4 (5.59 mol%) CH 3 SO 2 NH 2 as an additive in t-butyl alcohol : H 2 O (1 : 1) 0°C, was completed in 66 h, furnished the protected derivative of nojirimycin 22. However, even aer purication by preparative thin layer chromatography (PTLC) it was difficult to obtain the pure product nojirimycin 22 and it was contaminated with some uncharacterized impurities due to which the peaks in NMR were not very distinct for analysis. But the LC-MS and HRMS were in good agreement with the desired product nojirimycin derivative 22. Similarly, by treating iminoglucal 17a with (DHQD) 2 AQN (5 mol%), with K 3 Fe(CN) 6 , K 2 CO 3, K 2 OsO 2 (OH) 4 (5.59 mol%) CH 3 SO 2 NH 2 in t-butyl alcohol : H 2 O (1 : 1) 0°C for 60 h, furnished the protected derivative of nojirimycin 23. Similar to nojirimycin derivative 22 in this case also it was difficult to purify the nojirimycin derivative 23 by PTLC and it was contaminated with some uncharacterized impurities. Nojirimycin derivative 22 and 23 can be readily converted to nojirimycin 1 and nojirimycin B 4 by known methods of Boc deprotection and dehydrogenation as reported in the synthesis of nojirimycin and deoxynojirimycin.
All the attempts for the dihydroxylation of the iminogalactal 17b by following the same conditions as that for iminoglucal 17a with (DHQD) 2 AQN as well as (DHQ) 2 AQN could not furnish the desired product. Probably we could reason that, the axial OBn group at C-4 in the case of iminogalactal 17b blocks the approach of Osmium from b-face and a-face is blocked by Boc group, which is already occupying the a-face (Boc group placed trans to C-4 OBn group to minimize steric interaction). The situation is different in iminoglucal 17a where the OBn group at C-4 is in equatorial position doesn't hinder the entry of osmium atom from either of the facial attack, also the Boc group maintains the more stable equatorial position in the plane of the piperidine ring without hampering the dihydroxylation process.
By following the known reaction conditions, generally, Boc deprotection is obtained in quantitative yields 10c and debenzylation are obtained in yields of 85% 8a utilizing assumption for nal deprotection (Boc deprotection and debenzylation) starting from glucolactam 7a 2-deoxynojirimycin 21a can be readily obtained in 76%, and in 22% starting from 2-deoxygluconolactone 9a. Similarly, nojirimycin 1 can be synthesized in 22% from 2-deoxygluconolactam 7a and in 6% starting from 2-deoxygluconolactone 9a. Likewise nojirimycin B or mannojirimycin 4 can be synthesized in 52% from 2-deoxygluconolactam 7a and in 15% starting from 2deoxygluconolactone 9a. Initially, once again we tried to ascertain the failure of the dihydroxylation reaction with iminogalactal 17b using DFT studies but we observed that incorporating the heavier Os atom and bulky ligands made the calculation process very slow and time-consuming. DFT calculations for this problem still deserves attention for some more interesting results which will be undertaken in the future.

Conclusions
We have successfully synthesized fagomine and 4-epi-fagomine from 2-deoxygluconolactone and 2-deoxygalactonolactone, respectively from chiral pool approach employing less expensive reagents and easy to handle reaction conditions. By slight variation in reaction condition employing Super-Hydride, we have synthesized iminoglycal and functionalized the C-1 and C-2 position of iminosugar, which can serve as a handle for the synthesis of various other biologically active molecules. The partially reduced product by Super-Hydride i.e. 2-deoxynojirimycin derivative can be utilized for the synthesis of epimers of natural products such as batzellasides. Thus, the use of Super-Hydride opens a new route for the synthesis of different iminosugars. Also, formal synthesis of nojirimycin, nojirimycin B, and 2-deoxynojirimycin has been achieved. From the DFT studies, we could reason the failure of the formation of 2deoxygalactonojirimycin via Super-Hydride reduction of 2deoxygalactonolactam to 2-deoxygalactonolactamol.

General methods
The FT-IR spectra were recorded on a FT-IR-8300 Shimadzu spectrometer and are reported in cm −1 . NMR spectra were recorded on Bruker ACF 200 or AV200 (200 MHz for 1 H NMR and 50 MHz for 13 C NMR) or AV400 (400 MHz for 1 H NMR and 100 MHz for 13 C NMR) or JEOL ECX 400 (400 MHz for 1 H NMR and 100 MHz for 13 C NMR) or Bruker DRX-500 (500 MHz for 1 H NMR and 125 MHz for 13 C NMR) spectrometers using CDCl 3 as solvent. Tetramethylsilane (0.00 ppm) served as an internal standard in 1 H NMR and CDCl 3 (77.0 ppm) in 13 C NMR, respectively. Chemical shis are expressed in parts per million (ppm) and coupling constants (J) in hertz (Hz). Mass spectra were recorded on LC-MS/MSTOF API QSTAR PULSAR spectrometer, samples introduced by infusion method using the Electrospray Ionization Technique (ESI). HRMS (ESI) of samples was taken on an Orbitrap (quadrupole plus ion trap) and TOF mass analyzer. Analytical thin-layer chromatography (TLC) were performed on 0.2 mm coated Merck pre-coated silica gel (EM 60-F254) plates. Visualization was accomplished with UV light (254 nm) and exposure to either ethanolic phosphomolybdic acid (PMA) or p-anisaldehyde-acetic acid-sulfuric acid charring reagent followed by heating. PTLC separations were carried out on 0.25 mm E. Merck silica gel plates (60F254). All the melting points reported are recorded in an open capillary using Büchi melting point apparatus B-540 and are uncorrected. Optical rotations were recorded on Jasco P-2000 polarimeter Na-lamp, l = 589 nm. Flash chromatography was performed with Combi-Flash R f 200i equipped with UV/VIS and ELSD, Isco Teledyne Inc., USA using RediSep® column (SiO 2 ). All other chemicals were of analytical grade. Chemical nomenclature was generated using ChemDraw.