Size- and charge-dependent non-specific uptake of PEGylated nanoparticles by macrophages

The assessment of macrophage response to nanoparticles is a central component in the evaluation of new nanoparticle designs for future in vivo application. This work investigates which feature, nanoparticle size or charge, is more predictive of non-specific uptake of nanoparticles by macrophages. This was investigated by synthesizing a library of polymer-coated iron oxide micelles, spanning a range of 30–100 nm in diameter and −23 mV to +9 mV, and measuring internalization into macrophages in vitro. Nanoparticle size and charge both contributed towards non-specific uptake, but within the ranges investigated, size appears to be a more dominant predictor of uptake. Based on these results, a protease-responsive nanoparticle was synthesized, displaying a matrix metalloproteinase-9 (MMP-9)-cleavable polymeric corona. These nanoparticles are able to respond to MMP-9 activity through the shedding of 10–20 nm of hydrodynamic diameter. This MMP-9-triggered decrease in nanoparticle size also led to up to a six-fold decrease in nanoparticle internalization by macrophages and is observable by T2-weighted magnetic resonance imaging. These findings guide the design of imaging or therapeutic nanoparticles for in vivo targeting of macrophage activity in pathologic states.


Introduction
As one of the most phagocytic cells in the human body, macrophages are among the first cells of the innate immune system to arrive at a site of injury, but also have been observed as permanent residents in certain organs, such as in the liver and bone marrow. [1][2][3] They function to clear pathogens and microbes, as well as host cell and matrix debris that are present at sites of tissue injury. Macrophages recognize and interact with this multitude of potential targets through a variety of mechanisms, including phagocytosis and receptor-mediated endocytosis. The latter is mediated primarily through pattern recognition receptors, which include toll-like receptors, the mannose receptor (CD206), and scavenger receptor A (CD204). [4][5][6] The polygamous nature of these pattern-recognition receptors is not restricted to natural ligands and targets. For example, CD204 has a wide range of molecular partners, leading to receptor-mediated endocytosis, distinct from the non-specific uptake due to pinocytosis. 7 For the purposes of this manuscript, we have defined this polygamous behavior as "non-specific" uptake or internalization. This is emphasized by evidence that CD204 has been shown to contribute to the non-specific uptake of nanoparticles surface-functionalized with carboxylic acids, antibodies, as well as synthetic polymers. 8,9 Therefore, the rational design of nanoparticles for in vivo use requires an application-driven minimization or optimization of such non-specific interactions between macrophages and synthetic nanoparticles. However, this area remains largely uninvestigated.
Non-specific interactions between macrophage receptors and nanoparticles may be dictated by a variety of characteristics, including particle size, shape, surface charge, and hydrophobicity, and facilitated by surface chemistry-specific complement activation on the nanoparticle. 10, 11 Doshi and Mitragotri treated macrophages at 4°C with a library of polystyrene microparticles exhibiting a variety of sizes and shapes to mimic bacterial dimensions, and observed optimal attachment for rod-shaped particles with the longest dimension at 2-3 µm. 11 However, the smallest particles investigated were in the range of 500 nm; work with such nanoparticles has yet to be extended to the sub-100 nm dimensional range, which is of interest in many in vivo applications. Raynal et al showed that macrophages exhibit size-dependent uptake of nanoparticles functionalized with dextran, but macrophages can interact directly with dextran, as their expression of a dextran receptor (SIGNR1) was later documented. 12,13 Therefore, in this work, we sought to investigate nonspecific uptake of synthetic nanoparticles by macrophages, extending the work of these earlier groups into sub-100 nm PEGylated nanoparticles. To our knowledge, this is the first investigation of the effects of nanoparticle size, surface chemistry, and charge on non-specific uptake by macrophages. The rationale for using a PEG-functionalized nanoparticle system to accomplish these objectives is that macrophages are unlikely to have specific receptors for poly(ethylene glycol) (PEG). Further, PEG can be easily modified to display various chemical functionalities, enabling the modulation of nanoparticle charge without significantly varying the bulk properties of the PEG coating. This is also a relevant model system for study because PEGylation of nanoparticles is commonly performed in order to render synthetic nanoparticles water-soluble and applicable for in vivo use. This is, in part, because PEG has been shown to discourage protein adsorption and opsonization on nanomaterial surfaces. 14 Therefore, we used block copolymers of poly(ethylene glycol)-bl-poly(propylene sulfide) (PEG-PPS), which are amphiphilic copolymers that are capable of forming micelles and stabilizing hydrophobic drugs and nanoparticles at their liquid, PPS core. 15,16 The incorporation of ultrasmall superparamagnetic iron oxides (USPIOs) into the micellar core of PEG-PPS block copolymers serves two functional purposes -enabling easy quantification of particle uptake through colorimetric assays, while also being a widely investigated contrast agent for T 2 -weighted magnetic resonance imaging (MRI). Through the use of a variety of materials processing techniques to form the micelles, including thin film hydration and direct hydration, the same starting PEG-PPS copolymers and iron oxide cores can lead to monodisperse micelles (PEG-PPS-USPIOs) exhibiting hydrodynamic diameters at 30 nm, 40 nm, or 100 nm. Additionally, PEG-PPS-USPIOs can be fashioned with different surface chemistries at the PEG terminus, enabling an examination of charge-dependent non-specific uptake of nanoparticles by the macrophages.
To demonstrate the utility of these studies, we evaluated a protease-activity MRI probe design against these results. To make activity probes, PEG chains containing a proteasecleavable peptide substrate were synthesized and conjugated to PPS, in order to fashion surfactants for the micellization of USPIOs. The resulting nanoparticles are "activatable" by protease activity through a $10 nm decrease in hydrodynamic diameter. Macrophages are therefore expected to internalize protease-treated nanoparticles differently than untreated nanoparticles. We hypothesize that these differences can be visualized via MRI with the aid of the contrast agent USPIOs encapsulated within the micelles. Taken together, the work presented here shows methods to design ideal nanoparticle dimensions and properties in order to better optimize nanoparticle behavior in vivo.

Materials and methods
All reagents were purchased from Sigma-Aldrich (St Louis, MO) and used as purchased unless otherwise noted below. Matrix metalloproteinase-9 (MMP-9), MMP-9 inhibitor, Fmoc-protected L-amino acids, and resins for solid-phase peptide synthesis were purchased from EMD Biosciences (Gibbstown, NJ). PEG reagents were purchased from Laysan Biosciences (Arab, AL). All dialysis supplies were ordered from Pierce Scientific (Rockford, IL) and used with modifications to the factory-provided protocol as indicated in the appropriate sections below. Copper transmission electron microscopy (TEM) grids with Formvar film and uranyl acetate were purchased from Electron Microscopy Sciences (Hatfield, PA). GIBCO ® RPMI-1640 medium, penicillinstreptomycin, and fetal bovine serum (FBS) were purchased from Life Technologies (Carlsbad, CA).
For Fourier transform infrared (FT-IR) spectroscopy, polymer samples were prepared by mixing with IR-grade KBr and pelleting on a KBr press (Specac, Slough, UK). FT-IR was performed on a Bruker Tensor 27 system (Billerica, MA).
Proton nuclear magnetic resonance (1H NMR) spectra were obtained at 400 MHz using a 9.4 Tesla Oxford magnet operated by a Bruker AV-400 console. The main NMR probe for the instrument is a 5 mm Z-gradient broadband inverse (BBI) probe with automatic tuning and matching capability (ATM).
Gel permeation chromatography (GPC) was performed on three resolving columns running in series (1 × TSKGel Alpha4000, 2 × TSKGel Alpha3000; Tosoh Bioscience, King of Prussia, PA) with dimethylformamide (DMF) + 0.1 M LiBr mobile phase. Columns were incubated at 60°C, and chromatograms were obtained with a Shimadzu SPD-10A UV detector and RID-10A refractive index detector (Shimadzu Scientific Instruments, Columbia, MD), and a Wyatt miniDAWN Treos multi-angle light scattering detector (MALS; Wyatt Technology, Santa Barbara, CA). Data collection and analysis was achieved through the Wyatt ASTRA software (v 5.3.4).

Encapsulation of USPIOs in PEG-PPS copolymers
Synthesis of hydrophobic, monodisperse USPIO core particles and their encapsulation in PEG-PPS copolymers was carried out as previously described. 16 In brief, USPIO cores of predictable diameters were first synthesized through thermal decomposition, by controlling the molar ratios of iron precursor to oleic acid introduced in the reaction feed (Supplementary Figure S1). A 1:2 mass ratio of dried hydrophobic USPIO cores to PEG-PPS polymers were then dissolved in toluene, vortexed to mix, sonicated for 5 seconds to break apart clumps, and then dried by rotary evaporation for 20 minutes. The dried polymer/USPIO mixture was then rehydrated in 3 mL of nanopure water and vortexed vigorously to suspend all particulates. Large clumps and byproducts were removed by magnetic pelleting, and the colloidal phase was collected and further centrifuged at 2500 g for 5 minutes to precipitate excess polymers. The supernatant is gently aspirated by pipette into fresh scintillation vials and stored at 4°C.
To fabricate fluorescent micelles, a 1:40:20.5 mass ratio of FITC-PEG-PPS:PEG-PPS: iron oxide cores was mixed and micellized as described above. Therefore, the overall mass ratio of polymers to iron oxides is preserved at 1:2 for all micellization procedures. To make "proximity-activated" USPIOs (PA-USPIOs) -which are able to respond to local MMP-9 activity, OA-USPIOs were encapsulated in MMP-9-cleavable mPEG-[M9C]-PEG-PPS polymers using the same protocol.

Pluronic ® -PPS nanoparticles and loading with USPIOs
Pluronic-stabilized PPS nanoparticles (NPs) were synthesized by inverse emulsion polymerization as described previously. 10,19 Pluronic F-127 (a block copolymer of polyethylene glycol and polypropylene glycol terminated by α and ω hydroxyl groups) was used alone or in combination with carboxyl-terminated Pluronic derivatized as previously described. 20 The hydrophobic core was stabilized by disulfide crosslinking of the linear PPS chains. 19 However, since crosslinking cannot reach completion, remaining free sulfhydryl groups on the NP surface were irreversibly capped by reaction with the alkylating reagent iodoacetamide. NP solutions were sterile-filtered, and then loaded with 3 nm USPIO cores through a direct hydration process. Then 100 µL of the hydrophobic OA-USPIOs (20 mg/mL in tetrahydrofuran [THF]) was added to 1 mL of the Pluronic-PPS NPs (15 mg/mL in water) with swirling, and was followed by removal of THF by rotary evaporation, and removal of non-encapsulated OA-USPIOs by filtration through 0.45 µm Teflon filters (Whatman Inc, Piscataway, NJ).

Nanoparticle characterization
Size and ζ-potential of NPs were investigated by dynamic light scattering (DLS) in a Malvern Zetasizer Nano-ZS with the reusable dip-cell kit (Malvern Instruments Ltd, Worcestershire, UK). For measurements of ζ-potential in submit your manuscript | www.dovepress.com Dovepress Dovepress serum media, nanoparticles were mixed with THP-1 growth medium and allowed to incubate at 37°C for 24 hours prior to DLS measurements. No further purification of the nanoparticles was performed. This is because the purification process ends up diluting the particles (along with the adsorbed proteins), and may lead to further protein exchange interactions with media used downstream of isolation procedures, as per the Vroman effect. 14 Therefore, in order to best mimic in vivo conditions, the nanoparticles were measured in the presence of serum. Measurements of hydrodynamic diameter demonstrated the presence of a peak at ,5 nm that corresponded to proteins, while nanoparticles could still be easily discerned within the 20-100 nm diameter range.
Transmission electron microscopy (TEM) was conducted on a Philips CM20 system (Amsterdam, the Netherlands) operating at 200 kV. Carbon film-backed copper grids were inverted onto droplets containing nanoparticle suspensions of interest and blotted dry. Images were collected using a CCD camera with AMT Image Capture Engine software (v 600.335h built on 29 Apr 2010; Advanced Microscopy Techniques, Danvers, MA), and sizing of the particles was automated using a particle analyzer on ImageJ software (v 1.43u). Images were thresholded, and then the built-in (Analyze Particles) function was used to measure the major and minor axes of the fit ellipses around each particle. After artificially discarding clumps of particles encompassed within single fit ellipses (usually identified by major and minor axes that were .10% different from one another), or ellipses drawn around globs in the carbon grid (usually identified by any dimension ,1 nm), the diameter of individual particles was taken to be the average of the major and minor axis.
For aqueous samples, nanoparticles on TEM grids were also counterstained with 3% uranyl acetate in water for 2 minutes, gently blotted dry, and dried in a vacuum desiccator for 2 hours prior to imaging.
The differentiation process leads to induction of cell adherence onto tissue culture polystyrene surfaces.
For nanoparticle co-incubation experiments, cells were washed once with phosphate buffered saline (PBS) to remove unbound cells, prior to addition of growth medium. The medium was supplemented with nanoparticles and fucoidan. Nanoparticle dosing was based on total iron concentration as measured through the colorimetric phenanthroline assay as previously described. 21 Final iron concentrations in the wells were calculated to be between 30 µM and 200 µM. For fucoidan competition experiments, media was supplemented with fucoidan to a final concentration of 0-500 µM.
At selected time points, cells were washed three times with PBS to remove unbound nanoparticles, and then lysed in 3 N HCl and 0.25% Triton X-100 for at least 2 hours. The strongly acidic environment also promotes solubilization of the endocytosed USPIOs via oxidation of the amphiphilic PEG-PPS shell on the nanoparticles into fully hydrophilic polymers, 19 as well as leaching and mineralization of the iron in the USPIO core. The cell lysate was analyzed for protein content using a commercial Lowry protein assay kit (Bio-Rad Laboratories, Hercules, CA), while iron content was measured using the colorimetric phenanthroline assay as previously described. 21 While the acidic conditions for the Lowry protein assay deviate significantly from the protocol described by the supplier (alkaline conditions), this does not significantly affect the sensitivity or results of the assay (Supplementary Figure S2).
Calculation of nanoparticle internalization was dependent upon nanoparticle type, as shown in Table 1. The rationale behind the two different measurement types is inherent to the loading efficiencies possible. Because PEG-PPS-USPIO samples are purified, all cell-nanoparticle interactions in experiments involving them involve an iron "tag". However, since Pluronic-PPS-USPIOs are a subpopulation of the nanoparticles used in this system, not all cell-nanoparticle interactions here involve the iron tag. Due to differing USPIO loading efficiencies across the different Pluronic-PPS surface chemistries available to us, an additional normalization method was required in order that resulting figures fully represented charge-dependent uptake of nanoparticles. The normalization of internalization data to the initially administered dose of iron was therefore used to report internalization of Pluronic-PPS-USPIOs (Table 1).
For cell viability experiments, cells were incubated for 24 hours with PEG-PPS-USPIOs, at a final iron dose of 30 µM, 60 µM, or 120 µM. After rinsing cells three times with PBS to remove unbound nanoparticles, they were submit your manuscript | www.dovepress.com Notes: PEG-PPS-USPIO internalization data was also represented as %ID/mg once in this manuscript ( Figure 3E) in order to facilitate comparison of results. Abbreviations: PEG, poly(ethylene glycol); PPS, poly(propylene sulfide); USPIO, ultrasmall superparamagnetic iron oxides.

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stained with a commercial calcein-AM/ethidium homodimer live/dead assay kit (Invitrogen, Carlsbad, CA), and quantified according to the manufacturer's instructions.

Protease-activatable nanoparticles
"Proximity-activated" USPIOs (PA-USPIOs) -which are able to respond to local MMP-9 activity, were formed as described for other PEG-PPS-USPIOs above. For protease experiments, 50 µL PA-USPIOs (iron dose = 600 µM) were incubated with 10 µL MMP-9 (final concentration = 2 µg/mL) in an aqueous buffer containing 0.1 M HEPES, 0.15 M NaCl, and 5 mM CaCl 2 (pH 7.2) for 24 hours at 37°C. For control experiments, PA-USPIOs were incubated with buffer only. Following cleavage, nanoparticles were added directly to cell cultures. The final concentration of iron and MMP-9 in the cell cultures were 120 µM and 400 ng/mL, respectively. In some control experiments, MMP-9 inhibitor was also added to the cell cultures, to a final concentration of 300 ng/mL.

Magnetic resonance imaging (MrI)
MRI was performed on a Varian 4.7 T horizontal bore imaging system. T 2 signal decay was measured using a Carr-Purcell-Meiboom-Gill (CPMG) spin-echo pulse sequence with N = 8 echoes with 6.5 ms echo spacing. The signal from each voxel at the eight imaging time points was fit to a mono-exponential signal decay model to determine T 2 for each voxel: A region of interest (ROI) was manually drawn using MATLAB (MathWorks, Inc, Natick, MA) for the first imaging time point and translated to the images from later echoes. The mean T 2 and standard deviation for each well was then calculated from all voxels within this ROI. Other imaging parameters included TR = 2 seconds, field of view = 22 mm × 22 mm, data matrix = 128 × 128, slice thickness = 1 mm, number of acquisitions = 24 (total scan time approximately 1 hour 45 minutes).
To prepare cells for MRI, the supernatant containing unbound nanoparticles in medium was aspirated and replaced with PBS, prior to scraping of the cells into the buffer (Corning Life Sciences, Lowell, MA). Cells were centrifuged into a pellet at 300 g for 5 minutes, and rinsed with PBS twice more. Cells were then fixed with 10% buffered formalin, gently mixed, and allowed to incubate for 30 minutes at room temperature before they were pelleted and imaged.

Results and discussion
The primary objective of this study was to investigate sizeand charge-dependent non-specific uptake of nanoparticles by macrophages. With the targeted size range being in the sub-100 nm hydrodynamic diameter range, the objectives required the synthesis of a library of highly monodisperse, water-soluble nanoparticles in order to reduce size overlap between different nanoparticle formulations and elucidate trends between size and uptake. Therefore, USPIO cores were synthesized by thermal decomposition in organic solvents, which led to oleic acidstabilized USPIOs (OA-USPIOs) of 3.0 ± 0.4 nm ( Figure 1A, n approximately 200) and 12.0 ± 1.0 nm ( Figure 1B, n . 400). Control over USPIO core diameters was accomplished by adjusting the molar ratios of oleic acid surfactant to iron pentacarbonyl precursor in the reaction feed, and to date, we have synthesized OA-USPIOs of up to 24 nm in diameter using this method (Supplementary Figure S1). These results extend previous work by Woo et al, 22 who showed the ability to synthesize particles from 5 nm to 19 nm in diameter using this same exact method. Additionally, we were also able to scale up this original synthesis and now are able to produce the uniform OA-USPIOs in 1 g amounts. To render the OA-USPIOs water-soluble, either a thinfilm hydration or a direct hydration method was employed, effectively encapsulating OA-USPIOs within micelles composed of amphiphilic PEG-PPS block copolymers (1.65 kDa PPS block, 4.2 kDa PEG block; Figure 1C-E). Prior to cell experiments, the micelles were sterile-filtered; size-number distributions of the completed USPIO-loaded micelles are shown in Figure 1F. However, due to the larger size of the 100 nm micelles, these materials tended to be caught in the Teflon filters and were thus used as synthesized. The 30 nm and 40 nm micelles were particularly stable in water and flocculated minimally even after storage for several months at room temperature ( Figure 1G). These two formulations were also extremely difficult to pellet by centrifugation or through the influence of an externally-applied 1 T neodymium magnet. The completed micelles exhibited ζ-potentials that were weakly anionic (Table 2), owing partly to the terminal mono-methyl ether group on the PEG block that is displayed on the nanoparticle surface.
Nanoparticles were next administered to THP-1 human leukemic macrophages in order to establish a quantitative basis for the remainder of the experiments, while also examining the kinetics of particle uptake. THP-1 cells were chosen for this study because uptake and processing of lipid nanoparticles by submit your manuscript | www.dovepress.com    THP-1 and primary human monocyte-derived macrophages is not significantly different between the two cell types. 23,24 We expected, therefore, that macrophage interactions with synthetic nanoparticles can be similarly modeled through this readily available, in vitro system.

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As an example, varying doses of the 100 nm PEG-PPS-USPIOs were administered to THP-1 macrophages. Because first-order rate equations are often used as governing equations in efforts to model receptor-mediated endocytosis of nanoparticles by macrophages, 25 the resulting 24-hour uptake profiles ( Figure 2A) were fit to first-order rate kinetic equations (Figure 2A and B). The successful curve-fit suggested that USPIO concentration is the primary determinant of uptake rate. The best-fit equations take the form: where [C] max represents the maximum possible concentration of iron in the cells and [C] is a measure of the accumulated iron content in the cells. As the fit equations show (Figure 2A) errors 25%-40%). Relative to the initial doses of USPIOs, macrophages receiving 30 µM, 60 µM, and 120 µM of iron endocytosed 8.4% ± 3.7%, 7.7% ± 3.2%, and 6.2% ± 0.9% of the maximum possible USPIOs, respectively. In order to ensure that the measurements excluded USPIO binding events not resulting in uptake, some experiments were also conducted at 4°C to block endocytosis, resulting in insignificant iron levels quantified in the lysates (Supplementary Figure S3). In addition, a live/dead cytotoxicity assay was also conducted in order to confirm that treatment of macrophages with the PEG-PPS-USPIOs resulted in minimal cell death (Supplementary Figure S4). Similar nanoparticle uptake kinetics were also observed for particles of smaller hydrodynamic diameters ( Figure 2C). The results also show that by mass, smaller nanoparticles are internalized less effectively than their larger counterparts. The 40 nm nanoparticles shown in this graph were based on a higher iron dose for easier visualization; however, the same nanoparticles, administered at the same 120 µM Fe dose as the other two samples, were internalized at 0.36 ± 0.55 µg/mg protein (curve not shown). Normalization of the 24-hour uptake data to the initially administered doses shows that THP-1 macrophages were able to internalize 1.1% ± 0.3%, 1.4% ± 2.3%, and 6.2% ± 0.9% of the 30 nm, 40 nm, and 100 nm PEG-PPS-USPIOs, respectively. When nanoparticle internalization is normalized to cell number, a 70% decrease in PEG-PPS-USPIO diameter corresponded with almost a ten-fold decrease in iron uptake per cell. This was supported by fluorescence microscopy experiments, where macrophages were treated with FITC-tagged nanoparticles ( Figure 2D), demonstrating the accumulation of 100 nm micelles within the macrophages. Despite longer exposure times at a lower magnification, the microscope was insufficiently sensitive to visualize the internalization of the 40 nm micelles by the macrophages. Taken together, these data suggest a positive correlation between nanoparticle size and their non-specific recognition and internalization by macrophages.
Given the lack of any specific targeting moieties on the micelle surface, this evidence suggested that within the nanoparticle size range investigated, macrophages were able to optimally recognize and internalize PEGylated nanoparticles of .100 nm diameter. Further, smaller nanoparticles seemed to experience significantly less non-specific uptake by the macrophages. One of the mechanisms of uptake is likely through receptor-mediated endocytosis via CD204 -as PEG-PPS-USPIO internalization can be effectively blocked by co-administration of nanoparticles with fucoidan, which is a well-known CD204 ligand ( Figure 2E). 4 We next investigated the effects of nanoparticle charge on non-specific uptake. Because the sub-40 nm nanoparticles provided a satisfactorily minimal baseline uptake over 24 hours, we opted to focus on nanoparticles of this size for this section of the study. End-carboxylated, -aminated, and -thiolated Pluronic were used as surfactants in inverse emulsion polymerization as described previously. 10 The resulting Pluronic-PPS nanoparticles were loaded with USPIOs via direct hydration ( Figure 3A-C), and delivered to THP-1 macrophages under the same conditions described for the other cell experiments above. Since Pluronic polymers are PEG-containing block co-polymers, the properties of Pluronic-PPS are not very different from those of PEG-PPS used in the other studies shown here, and, in effect, still produce PEG-PPS-coated USPIOs. Pluronic-PPS enables facile synthesis schemes necessary to produce the various endfunctionalized polymers used in this work that would otherwise be more difficult to generate from PEG-PPS coatings. 19 In order to account for differences in USPIO loading efficiencies across the library of Pluronic-PPS nanoparticle formulations, uptake was not only reported as [Fe]/[Protein] as above, but further normalized to initial doses of iron and reported as percent injected dose/protein (%ID/mg protein; Figure 3D, Table 1). We hypothesized that this system would enable us to parse out the roles of surface charge from size on nanoparticle internalization, leading us to identify the sensitivity of size and charge on nanoparticle non-specific uptake by macrophages.
The Pluronic-PPS-USPIOs initially exhibited surface charges from −23 mV up to +9 mV, but following incubation in 10% serum media for 4 hours, all nanoparticle formulations experienced significant changes in ζ potential ( Figure 3D). Therefore, while the ζ potential of the nanoparticles was tunable to some extent by varying the surface chemistry of the nanoparticles, electrostatic interactions with serum proteins and components, as well as protein adsorption and opsonization processes contributed to significant changes in nanoparticle properties. The addition of serum into the incubation medium for these studies is intended to reflect an interaction environment that includes important components of the in vivo environment. Since there is no opportunity for nanoparticle purification following intravenous injection, we elected to allow nanoparticle interaction with serum proteins during ζ potential measurements, and later on, incubation with THP-1 cells. One outcome of this approach, and equally true in vivo, is the modulation of initial nanoparticle ζ potential by serum protein adsorption. These processes have been studied in detail for the Pluronic-PPS nanoparticle system, as submit your manuscript | www.dovepress.com Dovepress Dovepress agglomeration, as no flocculation or sedimentation was observed in any of the samples following treatment with serum. This observation was true of all nanoparticle formulations regardless of surface chemistry, possibly owing to the colloidal stability of Pluronic-PPS nanoparticles as shown previously. 19 The two formulations that were most efficiently internalized were the nanoparticles displaying the terminal OH (12% ± 5% ID/mg protein) and the 10%COOH/90% OH/SH (11% ± 5%/mg). Uptake correlated with nanoparticle charge as measured in serum, yielding a parabolic trend with maximum uptake observed for cationic and strongly anionic nanoparticles ( Figure 3D, R 2 = 0.94, inset). However, because interactions with serum compressed the range of nanoparticle ζ potentials, we were unable to experimentally explore uptake of the nanoparticles beyond the −10 mV to 0 mV range. Despite the narrow window of ζ potentials covered by the data, the trends suggest that ζ-potential of all nanoparticle formulations (color-coded by surface chemistry) was originally measured in PBS following synthesis, and again following incubation in 10% serum media. While modulation of surface chemistry allows for a wide range of ζ-potentials, this range is compressed due to interactions between nanoparticles and media components. Uptake of nanoparticles correlated with their surface charge as measured in media (inset; red dotted boxes indicate source of data for x-axis), according to a parabolic distribution. To account for differences in USPIO loading efficiency across the different Pluronic-PPS nanoparticle formulations, nanoparticle uptake was normalized to the initial dose administered as well as cell content indirectly, via a protein assay. Error bars indicate standard deviation for three independent experiments. (E) Cell internalization data is plotted versus nanoparticle ζ-potentials measured in 10% serum media (solid squares).
In order to determine which nanoparticle feature may be more determinant of non-specific interactions with macrophages, the effects of nanoparticle diameter have also been plotted for comparison (open squares). Abbreviations: OA-USPIOs, oleic acid-stabilized ultrasmall superparamagnetic iron oxides; PBS, phosphate buffered saline; PPS, poly(propylene sulfide); TEM, transmission electron microscopy.
reported by Thomas et al. 10 In particular, varying the surface chemistry of this nanoparticle system influenced the ability of the nanoparticles to become functionalized with the C3 complement proteins. 10 More generally, this phenomena is well known in the synthetic gene delivery field, in which cationic nanoscale carriers of pDNA or siRNA rapidly interact with albumin and other serum proteins in vivo, and is consistent with the findings reported here. 26 This is significant because many consider that a minimum ζ potential of ±30 mV is necessary in order to form stable nanoparticle suspensions. 27 Because electrostatic interactions and adsorption processes between serum proteins and the nanoparticle surface are inevitable following in vivo administration, higher ζ potential magnitudes may actually promote these processes, and in turn, opsonization processes ultimately leading to nanoparticle clearance from the bloodstream. The observed decrease in the magnitude of the nanoparticle ζ potentials did not correspond with increased submit your manuscript | www.dovepress.com Dovepress Dovepress non-specific uptake of nanoparticles may be promoted by nanoparticle cationicity or high anionicity. This is consistent with previous observations. 13 Uptake of nanoparticles in serum was minimized in the range of ζ potentials from −9.0 mV to −3.5 mV. A threefold increase in uptake was measured for identically sized nanoparticles having ζ potentials in serum from −3.5 mV to −0.8 mV, representing a 77% decrease in anionicity. In comparison, a four-fold change in uptake was observed for a 60% decrease in PEG-PPS-USPIO diameter (100 nm to 40 nm). Over these ranges and conditions, macrophage uptake of these nanoparticles is 42% more sensitive to size than to ζ potential ( Figure 3E).
To expand on this conclusion, we synthesized PEG-PPS-USPIOs containing an MMP-9-degradable peptide (M9C) within the PEG chain ( Figure 4A and B). This design results in particles that respond to active MMP-9 in the environment by releasing a layer of PEG, effectively leading to a decrease in nanoparticle diameter. Probes for MMP-9 activity are of wide interest because of the upregulation of MMP-9 in the progression of atherosclerosis. [28][29][30] Based on the studies described earlier, we hypothesized that this experimental contrast agent would experience less uptake by macrophages following treatment with MMP-9, relative to the as-synthesized, intact form.
Because these changes in nanoparticle internalization may be due to MMP-9-mediated modifications on the cell membranes, a series of control experiments were also performed, using non-cleavable, 40 nm PEG-PPS-USPIOs (Supplementary Figure S5). In these experiments, co-administration of non-cleavable nanoparticles with MMP-9 did not lead to significant differences in nanoparticle internalization. Further, co-administration with a MMP-9 inhibitor also did not affect internalization. Taken together, the results suggest that the variations in PA-USPIO internalization by the THP-1 cells were attributable to the size of the nanoparticles, as the nanoparticle ζ potentials did not vary significantly before versus after treatment with MMP-9. Further, cellular capacity for nanoparticle internalization was unaffected by exposure to protease.
To determine if these MMP-9-dependent differences in nanoparticle internalization result in statistically significant changes in sample T 2 relaxation, and therefore, clinically relevant detection of MMP-9 activity in cell samples, 60 nm PA-USPIO-treated cells were pelleted and imaged on a 4.7 T magnetic resonance imaging scanner ( Figure 4F). Through the use of a CPMG spin-echo pulse sequence, MMP-9-treated PA-USPIOs appeared to exhibit higher mean gray intensities versus cells incubated with untreated PA-USPIOs.
While the PA-USPIOs exhibited T 2 = 4.82 ± 0.02 ms, the PA-USPIOs on the macrophages exhibited T 2 = 23.2 ± 3.5 ms. These rather strong changes in T 2 are somewhat surprising assuming that approximately 1% of the administered dose was taken up by the macrophages as measured in the earlier sections of this work. This implies that for each imaging slice, the concentration of iron responsible for T 2 signal modulation within that slice is about 100-fold less in the nanoparticle-treated cell samples versus the positive control. To quantify this phenomenon, the calculated values above (from first-principle measurements) can be plugged into the R 2 relaxivity equation: in order to produce a measure of how effective the USPIOs are in modulating the local negative contrast. Given that measured T 2 in the cell samples is only approximately five times higher than the measurements in the positive control, the iron concentration in the cell samples would need to be about a fifth of the concentration in the positive control in order to maintain the same R 2 value. As we have seen, this submit your manuscript | www.dovepress.com (E) Buffer-treated or MMP-9-pretreated nanoparticles were delivered to THP-1 macrophages for 24 hours in standard growth medium. As a control, PBS was used in place of the nanoparticles. For both PA-USPIO formulations tested, the decrease in nanoparticle size following MMP-9 treatment results in less effective nanoparticle internalization by the macrophages. Error bars represent standard deviations from three to six independent experiments. (F) T 2 -weighted MrI of THP-1 cells treated with MMP-9-cleaved PA-USPIOs appeared brighter than cells incubated with untreated PA-USPIOs, indicating that less cleaved nanoparticles were internalized by the macrophages versus the untreated PA-USPIOs. Note: *P , 0.05 by Student's t-test. Abbreviations: DLS, dynamic light scattering; MMP-9, matrix metalloproteinase-9; PA-USPIOs, proximity-activated ultrasmall superparamagnetic iron oxides; MrI, magnetic resonance imagery; PBS, phosphate buffered saline; PEG, poly(ethylene glycol); PPS, poly(propylene sulfide).

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is hardly the case, and based on our data, we can conclude that the R 2 values in the cell sample would have to be on the order of 20-fold larger than the R 2 of the free-floating PA-USPIOs. These results indicate that following internalization by the macrophages, the PA-USPIOs are being manipulated in such a way that increases their ability to exert T 2 contrast. This phenomenon can be explained by previous observations that aggregated or clustered superparamagnetic nanoparticles result in higher R 2 versus fully dispersed, singlet nanoparticles. 16,31 Others have demonstrated via TEM that following endocytosis of iron oxide nanoparticles, macrophages can process the particles into lysosomes, where dense clusters of particles can usually be observed. 32 submit your manuscript | www.dovepress.com

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Taken together, these other observations help explain how even a small amount of nanoparticle uptake results in a marked change in T 2 contrast in the system.

Conclusion
PEGylated nanoparticles are internalized by macrophages in a size-dependent fashion for diameters between 30 nm and 100 nm. Charge-uptake relationships were investigated by varying the surface properties of nanoparticles. While the data supports the possibility that cationic and strongly anionic nanoparticles may be internalized most effectively, within the ranges investigated, nanoparticle size, not charge, is a stronger determinant of non-specific uptake by macrophages. Based on this information, an MMP-9-sensitive nanoparticle was developed that decreases in size following treatment with MMP-9. Macrophages respond to MMP-9-treated nanoparticles in a predictable fashion, and cleaved nanoparticles were consistently phagocytosed less efficiently than their untreated counterparts, demonstrating the effects of dynamic nanoparticle size modulation on macrophage uptake. These MMP-9-induced differences in uptake are also detectable via MRI. Despite the low levels of overall uptake over the 24 hour incubation periods (#1% initially administered dose), a significant increase in macrophage R 2 was observed. Presumably, and consistent with quantitative analysis, the clustering of nanoparticles into endosomes following endocytosis results in an increase in nanoparticle R 2 , providing amplification of negative MR image contrast. The results presented here inform the design of nanoparticles to target or evade macrophages in future in vivo applications.