A case of oligoasthenoteratozoospermia with AZFc deletion and persistent oxidative stress

Even though the exact function of most genes on the Y chromosome is still not known, the evidence suggest that they are directly involved in male fertility regulation.[1] The Y chromosome AZFc region deletions result in severe oligospermia or azoospermia. The candidate genes on AZFc region have testis specific expression; however, their exact role is still not known.[2] DAZ genes (major spermatogenic candidate on AZFc) encode proteins located in human late spermatids and in sperm tails, which suggests a function of DAZ proteins in the RNA metabolism of late spermatids, mostly the storage or transport of testis-specific mRNA, the translation of which is repressed until the formation of mature spermatozoa. Hence, deletion of DAZ genes was supposed not to interfere with sperm maturation but to result in a gradual reduction of mature spermatozoa.[3] Oligo asthenoteratozoospermia (OAT) is a condition of abnormally low sperm count, motility, and high count of misformed spermatozoa in the ejaculate. Of all the OAT patients, approximately 30%, are diagnosed as idiopathic. OAT is rarely associated with Y chromosome microdeletion.[4] In the present study, we report a case of infertile man with a clinical picture of OAT associated with AZFc deletion and persistent elevated levels of reactive oxygen species (ROS) even after antioxidant therapy.


Introduction
Even though the exact function of most genes on the Y chromosome is still not known, the evidence suggest that they are directly involved in male fertility regulation. [1] The Y chromosome AZFc region deletions result in severe oligospermia or azoospermia. The candidate genes on AZFc region have testis specific expression; however, their exact role is still not known. [2] DAZ genes (major spermatogenic candidate on AZFc) encode proteins located in human late spermatids and in sperm tails, which suggests a function of DAZ proteins in the RNA metabolism of late spermatids, mostly the storage or transport of testis-specific mRNA, the translation of which is repressed until the formation of mature spermatozoa.
Hence, deletion of DAZ genes was supposed not to interfere with sperm maturation but to result in a gradual reduction of mature spermatozoa. [3] Oligo asthenoteratozoospermia (OAT) is a condition of abnormally low sperm count, motility, and high count of misformed spermatozoa in the ejaculate. Of all the OAT patients, approximately 30%, are diagnosed as idiopathic. OAT is rarely associated with Y chromosome microdeletion. [4] In the present study, we report a case of infertile man with a clinical picture of OAT associated with AZFc deletion and persistent elevated levels of reactive oxygen species (ROS) even after antioxidant therapy.

Case Report
The patient was a 35-year-old man, presented with primary infertility of five years duration. Y-chromosomal microdeletions are associated with severe oligozoospermia or azoospermia. AZFc microdeletions have been always associated with severe oligozoospermia or azoospermia with a rare occurrence in individuals with other infertility phenotypes. We report here a rare case of an infertile man carrying AZFc deletion, whose semen picture is oligoasthenoteratozoospermia complexed with seminal oxidative stress. Anti-oxidant therapy could make no change in either oxidative stress biomarker levels of semen, seminal parameters or serum hormone levels. Therefore, oligoasthenoteratozoospermia in the present case correlates with AZFc deletion, and high content of abnormal sperm eventually might be responsible for persistently elevated reactive oxygen species levels.
Understanding the function of genes in AZFc region could help decipher the exact cause of the phenotype in such cases.  were measured as described elsewhere. [5] Values for sperm count, morphology, and motility were low [ Table 1].
Seminal plasma levels of lipid peroxides and protein carbonyl group (PCG) were elevated, whereas vitamin A, vitamin E, superoxide dismutase (SOD), and catalase were lower than the normal limit. The level of vitamin C was within normal limits [ Table 1]. These findings were confirmed by a second set of tests performed on a different occasion. In view of these findings, a presumptive diagnosis of oligoteratoasthenozoospermia (OAT) associated with oxidative stress was made.
The patient was prescribed Anoxee supplement oncedaily for a period of two months followed by Mucuna pruriens seeds powder at the dose of five gm/day orally for three months to alleviate the oxidative stress. We have earlier demonstrated effectiveness of M. pruriens in treatment of oxidative stress associated male infertility. [5] At the end of therapy, the laboratory analyses were repeated [ Table 1]. None of the parameters under investigation showed a desired improvement. Before deciding upon further treatment, molecular analyses of Y-chromosome were carried out according to the guidelines suggested by European Academy of Andrology (EAA). [6] The results revealed intact AZFa and AZFb, whereas AZFc was completely deleted [ Figure 1].
Further analyses using another set of primers confirmed the b2/b4 deletion pattern of AZFc. The two markers sY254 and sY255 are specific to the DAZ (deleted in azoospermia) gene, which is normally present in four copies in AZFc region. [6] The analysis suggested that abnormal sperm production was associated with AZFc deletion. in 5-10% of infertility cases. [6] The above studies have suggested that AZFc deletion is largely associated with reduced sperm concentration (oligozoospermia  [7] ). The spermatogenic factors responsible for elevated oxidative stress are abnormal morphology, [8] reduced sperm motility, [9] and lower sperm count. [10] Abnormal sperm morphology was well-known cause of