BRCA1/p220 loss triggers BRCA1-IRIS overexpression via mRNA stabilization in breast cancer cells.

BRCA1/p220-assocaited and triple negative/basal-like (TN/BL) tumors are aggressive and incurable breast cancer diseases that share among other features the no/low BRCA1/p220 expression. Here we show that BRCA1/p220 silencing in normal human mammary epithelial (HME) cells reduces expression of two RNA-destabilizing proteins, namely AUF1 and pCBP2, both proteins bind and destabilize BRCA1-IRIS mRNA. BRCA1-IRIS overexpression in HME cells triggers expression of several TN/BL markers, e.g., cytokeratins 5 and 17, p-cadherin, EGFR and cyclin E as well as expression and activation of the pro-survival proteins; AKT and survivin. BRCA1-IRIS silencing in the TN/BL cell line, SUM149 or restoration of BRCA1/p220 expression in the mutant cell line, HCC1937 reduced expression of TN/BL markers, AKT, survivin, and induced cell death. Collectively, we propose that BRCA1/p220 loss of expression or function triggers BRCA1-IRIS overexpression through a post-transcriptional mechanism, which in turn promotes formation of aggressive and invasive breast tumors by inducing expression of TN/BL and survival proteins.

Apoptosis evasion allows further transforming mutations to accumulate in cancer cells and increase the possibility of disease progression and/or resistance to therapy [12,13]. About 50% of breast cancers carry dysfunctional p53 [14], and thus fail to arrest the cell cycle when damaged becoming chemo-drug resistant [14]. Similarly, ~40% of breast cancers show increase AKT kinase activity [15] and are apoptosis and chemodrug resistant [16,17].
Here, we show that BRCA1-IRIS overexpression in breast tumor cells is, at least partially, BRCA1/ p220-dependent. BRCA1/p220 silencing in HME cells downregulated expression of the RNA destabilizing proteins, AUF1 and pCBP2, that bind to BRCA1-IRIS mRNA 3`-UTR and destabilize it. BRCA1-IRIS overexpression in HME cells triggered expression of the TN/BL markers, cytokeratins 5 and 17 (CK5 and 17), p-cadherin (CDH3), EGFR and cyclin E [35] as well as expression and activation of the survival factors, AKT and survivin. BRCA1-IRIS silencing or BRCA1/p220 overexpression in BRCA1/p220-mutant or TN/BL cancer cell lines reduced expression of these TN/BL markers, AKT and survivin and induced cell death. Our data show that BRCA1/p220 loss of expression or function generates aggressive breast cancer cells, in part, by upregulating BRCA1-IRIS expression, implying that chemotherapeutic targeting of BRCA1-IRIS could be pursued for breast cancer patients with BRCA1/p220-associated or TN/BL diseases.

BRCA1-IRIS overexpression in BRCA1/p220 none-/low-expressing breast cancer cells
We showed earlier an inverse correlation between BRCA1-IRIS and BRCA1/p220 expressions in breast cancer cell lines [30]. Here, we confirmed that by analyzing proteins and RNAs isolated from several exponentially growing breast cancer cell lines and 2 normal HME cell lines. As expected western blot analysis using mouse monoclonal antibodies [30,36] and real time RT/qPCR, respectively, showed that in normal HME cell lines expressing high levels of BRCA1/p220 protein ( Figure 1A) and mRNA ( Figure 1B), BRCA1-IRIS protein ( Figure 1A) and mRNA ( Figure 1B) levels were significantly lower. In contrast, in sporadic or BRCA1/ p220-mutant (e.g., HCC1937), low or none BRCA1/p220expressing cell lines, respectively (see figure 1A and 1B), BRCA1-IRIS mRNA and protein expression significantly increased ( Figure 1A and 1B).

BRCA1/p220 does not affect BRCA1-IRIS protein stability
To understand the underlying molecular mechanism behind this inverse relationship, we considered three mutually exclusive scenarios. BRCA1/p220 could directly or indirectly; a) suppress BRCA1-IRIS gene transcription, b) decrease BRCA1-IRIS mRNA stability, or c) trigger BRCA1-IRIS protein degradation (BRCA1/p220 forms an E3 ligase with BARD1, see [9,10]). To distinguish between these possibilities, BRCA1/p220 or BARD1 were silenced in HME cells for 72h (see Figure 2A, far right panels) and cells were exposed to 10µM of cycloheximide (protein synthesis inhibitor) during the last 24h.
The levels of BRCA1-IRIS and actin proteins were measured using western blot on proteins isolated from these cells using sonication. All data were normalized to actin protein level in siLuc/no cycloheximide treated cells, which was taken as 1 (Figure 2A, left). As expected BRCA1-IRIS protein level decreased following cycloheximide treatment in control-, BARD1-and BRCA1/p220-silenced cells (Figure 2A, left). In the absence of cycloheximide, however, BRCA1-IRIS protein level was higher in BRCA1/p220-silenced cells, compared to control and BARD1-silenced cells (Figure 2A, left). Moreover, the levels of BRCA1-IRIS and GAPDH mRNAs was measured using real-time RT/qPCR on RNAs isolated from these cells. All data were normalized to GAPDH analysis of BRCA1-IRIS and BRCA1/p220 in immortalized normal HME cell lines (HME1 and HME2), sporadic and one inherited (HCC1937) breast cancer cell lines. The RNA levels in (B) are normalized to the levels of GAPDH found in each cell line. www.impactjournals.com/oncotarget mRNA level in siLuc/no cycloheximide treated cells, which was taken as 1 ( Figure 1A, right). BRCA1-IRIS mRNA level increased in control and BARD1-silenced cells following cycloheximide treatment only ( Figure  1A, right), whereas in BRCA1/p220-silenced cells before and after cycloheximide treatment ( Figure 1A, right). These data argue against an effect of BRCA1/p220 and/ or BRAD1 on the stability of BRCA1-IRIS protein. In fact, previously we were unable to detect any interaction between BRCA1-IRIS protein and BRCA1/p220 or BARD1 proteins in vitro or in vivo (see [30]).

BRCA1/p220 destabilizes BRCA1-IRIS mRNA
Next, we studied whether BRCA1/p220 affects BRCA1-IRIS mRNA stability (known to be controlled by elements in the 3`-UTRs of mRNAs). A BRCA1-IRIS cDNA that includes the entire 3`-UTR of BRCA1-IRIS (see [30]) was cloned in a doxycycline (Dox) inducible (left) analysis of the fold induction in BRCA1-IRIS protein normalized to actin or mRNA normalized to GAPDH mRNA, respectively in HME cells silenced (for 72h) from control (Luc), BRCA1/p220 and BARD1 and treated or not with cycloheximide during the last 24h. Data represent the means ± SD from triplicate, done three independent times, whereas ** is a p≤0.01. Far right panels show the effects of BRCA1/p220 (upper panels) and BARD1 (lower panels) siRNA on the expression of their cognate protein in HME cells. RT/qPCR analysis (B) or western analysis (C) of BRCA1-IRIS mRNA or protein, respectively in parental, uninducible IRISa and inducible IRISb and IRISc HME cell lines following control or BRCA1/p220 silencing for 24, 48, 72 or 168h. Data in (B) represent the means ± SD from triplicate, done three independent times, whereas * is a p≤0.05 and ** is a p≤0.01. Right panels in (B) show analysis for BRCA1-IRIS overexpression in the different inducible cell lines (upper panels), and the effect of BRCA1/p220 siRNA on the expression of BRCA1/p220 protein at 0, 72 and 168h (lower panels). mammalian expression vector, infected in HME cells and one uninducible (IRISa) and two inducible (IRISb and c) clones were selected to study further ( Figure 2B, right upper panel). We reasoned that since BRCA1-IRIS is expressed in these cells from an exogenous promoter, they should be a good system to explore whether BRCA1/ p220 affects BRCA1-IRIS expression by a transcriptional or post-transcriptional mechanism. Thus parental, IRISa, b and c were grown in the absence or presence of Dox (2µg/ml), in the presence of Dox but cells were transfected with BRCA1-IRIS or BRCA1/p220 siRNAs. RNAs and proteins (using sonication) were isolated at 24, 48, 72 or 168h post-siRNA transfection and the expression of BRCA1-IRIS mRNA and protein in each treatment was measured using real time RT/PCR ( Figure 2B, left) and western blot ( Figure 2C), respectively.
In the absence of Dox the 4 cell lines expressed normal levels of BRCA1-IRIS mRNA (black lines in Figure 2B, left) and protein ( Figure 2C). Dox induced BRCA1-IRIS mRNA (compare green to black lines in Figure 2B, left) and protein ( Figure 2C) expression in IRISb and c and not parental or IRISa starting at 24h. BRCA1-IRIS silencing decreased BRCA1-IRIS mRNA (see red lines in Figure 2B, left) and protein ( Figure 2C) in all cell lines (although less pronounced in induced IRISb and IRISc). The expression returned to pre-siRNA transfection levels at 168h (see red lines in Figure 2B, left and 2C). BRCA1/p220 silencing ( Figure 2B, right lower panels), however, increased BRCA1-IRIS mRNA (blue lines in Figure 2B, left) and protein ( Figure 2C) levels in all cell lines starting at 24h. These effects also disappeared at 168h post-siRNA transfection (blue lines in Figure 2B, Expression of BRCA1-IRIS mRNA in BRCA1/p220-silenced HME, MCF7 and MDAMB231 cells (left) or BRCA1/p220 overexpressing MDAMB468 and SKBR3 cells (right). (C) Expression of the indicated proteins in BRCA1/p220-, AUF1-, pCBP2-or HuR-silenced HME cells (upper panel) and the expression of BRCA1-IRIS in these cells (lower panels). (D) Expression of the indicated proteins (72h, left) or BRCA1-IRIS mRNA (at 25 PCR cycle, at 72h) in control-, AUF1-, or pCBP2-silenced MCF7 or MDAMB231 cells. Stability (t 1/2 ) of GAPDH (E) or BRCA1-IRIS (F) mRNAs as detected using real-time RT-qPCR analysis in BRCA1/p220-, AUF1-, pCBP2-or HuR-silenced HME cells treated with Actinomycin D (Act D) during the last 10h. Data are normalized to the levels of 18S rRNA in each experiment and are represented as a percentage of the mRNA levels measured at time 0 (before Act D addition) or 2, 4, 6, 8 and 10h after Act D addition using a semi-logarithmic scale. Data are presented as the means ± SD from triplicates, done three independent times (in all cases p≤0.001 compared to sicontrol cells). www.impactjournals.com/oncotarget (B) Plasmids carrying BRCA1-IRIS or c-fos 3`UTRs downstream of the luciferase gene in RSV-plasmid were transfecetd in HME with siGFP, siBRCA1/p220, siAUF1, sipCBP2 or siHuR and the luciferase activity expressed from these different plasmids was measured using luminometer. Data are represented as means ± SD from triplicates done three independent times (in all cases p≤0.01 compared to RSV-Luc alone). (C) Representative PCR analyses of the binding of BRCA1-IRIS mRNA to AUF1, pCBP2, HuR in untreated HME cells cytoplamsic proteins (polysomes), while binding only to HuR in BRCA1/p220-silenced HME cells. (D) Immunoprecipitation of the indicated proteins in BRCA1/p220-silenced HME cells (left), or from BRCA1/p220-reconstituted HCC1937 cells (right). (E) Immunoprecipitated mRNPprotein complex as percentage of input using AUF1, pCBP2, HuR and BRCA1/p220 antibodies and IgG from cells transfected with control or BRCA1/p220 siRNA. (F) Representative PCR analyses of the binding of AUF1 and pCBP2 to BRCA1-IRIS mRNA in BRCA1/p220silenced HME cells (upper) or BRCA1/p220-overexpressing HCC1937 (lower) cytoplasmic proteins (polysomes). left and 2C) when the effect of BRCA1/p220 siRNA disappeared (see Figure 2B, right lower panels).
To ascertain that these effects are dependent on the 3`-UTR of BRCA1-IRIS mRNA and not an artifact from the plasmid 5`-UTR (known inducer of mRNA translation), BRCA1-IRIS + 3`-UTR cDNA was cloned into several other mammalian expression vectors. Transient transfection of any of the plasmids into HME cells with BRCA1/p220 siRNA led to stabilization of the BRCA1-IRIS mRNA (not shown). We thus concluded that BRCA1/p220 loss stabilizes BRCA1-IRIS mRNA through an effect on its 3`-UTR.

Identification of BRCA1/p220 induced transacting proteins that de-stabilize BRCA1-IRIS mRNA
In an attempt to identify whether BRCA1/p220 has an effect on mRNA stability, we searched recently performed gene expression microarray data comparing BRCA1/p220-expressing to BRCA1/p220-silenced HME cells for 3`-UTR binding and destabilizing proteins. Using this approach we found that the expressions of the mRNA 3`-ITR binding and destabilizing proteins, AUF-1 and pCBP2 (see introduction) were significantly decreased in BRCA1/p220-silenced HME cells (data not shown).
Compared to control treated cells, BRCA1/p220 silencing (72h) significantly reduced AUF1 and pCBP2 but not HuR protein levels in HME, MCF7 and MDAMB231 cells, and its overexpression (48h) in MDAMB468 and SKBR3 cells significantly enhanced AUF1 and pCBP2 but not HuR protein levels (see Figure 3A, right). In contrast, BRCA1/p220-silencing increased the level of BRCA1-IRIS protein in HME, MCF7 and MDAMB231 to different degrees (see Figure 3A, left), whereas its overexpression significantly decreased BRCA1-IRIS protein expression in MDAMB468 and SKBR3 cells ( Figure 3A, right). This was also confirmed at the mRNA level. Indeed in real-time RT/PCR and after normalization to the level of GAPDH mRNA in each cell line, we found that BRCA1/ p220 silencing increased BRCA1-IRIS mRNA in HME, MCF7 and MDAMB231 cells starting at 24h ( Figure  3B, left) until 72h, but returned to control levels at 168h ( Figure 3B, left). BRCA1/p220 overexpression, on the other hand, decreased the level of BRCA1-IRIS mRNA in MDAMB468 and SKBR3 cells starting at 24h and thereafter ( Figure 3B, right).

BRCA1/p220 effect on BRCA1-IRIS is posttranscriptional and not transcriptional
To rule out any effect of BRCA1/p220 on BRCA1-IRIS gene transcription and to directly assess the effect of BRCA1/p220, AUF1 or pCBP2 on BRCA1-IRIS mRNA stability, BRCA1-IRIS mRNA half-life (t 1/2 ) was analyzed in control-, BRCA1/p220-, AUF1-, pCBP2-or HuRsilenced (72h) HME cells following exposure to the de novo transcription inhibitor actinomycin D (Act D) during the last 10h. Total RNAs were collected at 2, 4, 6, 8 and 10h after Act D treatment and analyzed by real-time RT/ qPCR for BRCA1-IRIS and GAPDH expression.

Identification of destabilizing cis-acting elements in BRCA1-IRIS mRNA 3`-UTR
Indeed, in silico search of BRCA1-IRIS 3`-UTR showed the presence of several cis-acting putative ARE consensus binding elements for AUF1 (class II AU rich binding motifs, see red in Figure 4A) and for pCBP2 (C-rich binding motifs, see green in Figure 4A), suggesting that the 3`-UTR destabilizes BRCA1-IRIS mRNA, in vivo. To experimentally confirm that, the entire BRCA1-IRIS 3`-UTR was cloned downstream of the luciferase coding region in the RSV-Luc plasmid (see atop of Figure 4B). The 3`-UTR of c-fos (known ARE containing) was also www.impactjournals.com/oncotarget cloned downstream of the luciferase coding region in the same plasmid to be used as positive control. Backbone plasmid transfection in HME cells produced high-level basal luciferase expression ( Figure 4B). Introducing BRCA1-IRIS or c-fos 3`-UTR downstream of the luciferase gene in this plasmid abolished that expression when cotransfected in HME cells with siGFP and siHuR (negative controls, Figure 4B), but not when co-transfected with BRCA1/p220, AUF1 or pCBP2 siRNA ( Figure 4B). Taken together these data suggest that like c-fos 3`-UTR, BRCA1-IRIS 3`-UTR carry AUF1 and pCBP2 binding and mRNA destabilizing motif(s).
To measure endogenous association between these trans-acting factors and the cis-acting elements in BRCA1-IRIS 3`-UTR, cytoplasmic proteins (polysomes) were collected 72h after control-or BRCA1/p220-silencing in HME cells in conditions that maintain RNA stability and were processed for immunoprecipitation with BRCA1/ p220, AUF1, pCBP2, HuR or IgG (negative control) antibodies. All immunoprecipitated samples were DNaseI digested to ensure they were free from any genomic contaminations before they were RT/PCR interrogated for BRCA1-IRIS mRNA. GAPDH mRNA (none target) was not immunoprecipitated by any antibody (Figure 4C), and no PCR amplification was detected when no reverse transcriptase (RT) was added to the reactions ( Figure 4C). In siLuc-transfected cells, AUF1, pCBP2, HuR and not IgG or BRCA1/p220 antibodies co-immunoprecipitate BRCA1-IRIS mRNA ( Figure 4C). This association was abolished when BRCA1/p220 was silenced ( Figure 4C).
The amounts of BRCA1-IRIS mRNA immunoprecipitated from control or BRCA1/p220 silenced HME cells by IgG, AUF1, pCBP2, HuR and BRCA1/p220 antibodies were compared to the total BRCA1-IRIS mRNA in polysome extracts using RT/qPCR. While equal amount of BRCA1-IRIS mRNA was immunoprecipitated with AUF1, pCBP2 and HuR antibodies from control treated cells (~30-35% of total BRCA1-IRIS polysomic mRNA see siLuc in Figure 4E), the amount immunoprecipitated by AUF1 and pCBP2 dropped to <10%, while the amount immunoprecipitated by HuR antibody increased to ~50% in BRCA1/p220-silenced cells ( Figure 4E). In line with that, from polysome extracts, Sp1 antibody immunoprecipitated no BRCA1-IRIS mRNA from controls, BRCA1/p220-silenced HME cells or BRCA1/ p220-overexpressing HCC1937 cells ( Figure 4F). BRCA1/p220 silencing in HME cells decreased, whereas BRCA1/p220 overexpression in HCC1937 cells increased the level of BRCA1-IRIS mRNA immunoprecipitated by AUF1 or pCBP2 antibody ( Figure 4F). These data show that BRCA1-IRIS mRNA is a target for the mRNA de/ stabilizing factors AUF1, pCBP2 and HuR in normal and breast cancer cells, and that AUF1, pCBP2 and not HuR expression is BRCA1/p220-dependent in these cells.

Loss of BRCA1/p220 enhances TN/BL phenotype via BRCA1-IRIS overexpression
The fact that BRCA1/p220-associated and TN/ BL breast cancers commonly show early onset and aggressive diseases expressing no-/low-BRCA1/p220, made us wonder whether BRCA1/p220 loss enhances the TN/BL phenotype via upregulating BRCA1-IRIS. To test this hypothesis, BRCA1/p220 was silenced in the BRCA1/p220-high-/BRCA1-IRIS-low expressing HME cells ( Figure 5A, upper panels). Total RNAs and proteins collected from these cells were then probed for the expression of the TN/BL markers, CK5, CK17, CDH3, EGFR and cyclin E [35]. As expected, BRCA1/p220 silencing upregulated the expression of the mRNAs and proteins of these markers (which otherwise expressed at low levels in HME cells, see Figure 5A, lower panels). More importantly, BRCA1-IRIS co-silencing in these cells decreased the expression of these markers ( Figure  5A, lower panels).
Furthermore, BRCA1-IRIS silencing in the TN/BL cell line, SUM149 that expresses high levels of BRCA1-IRIS, CK5, CK17, CDH3, EGFR, cyclin E but no BRCA1/ p220 ( Figure 5B) significantly decreased the expression of these TN/BL markers mRNAs and proteins ( Figure  5B). Whereas, HCC1937, the BRCA1/p220 mutant cell line that expresses high levels of BRCA1-IRIS and TN/ BL markers, but no wild type BRCA1/p220 ( Figure 5C), reconstitution with full-length BRCA1/p220 cDNA or silencing BRCA1-IRIS in them ( Figure 5C) decreased the levels of TN/BL markers and BRCA1-IRIS mRNA and protein ( Figure 5C). These data establish that BRCA1/ p220 loss of expression or function enhances the TN/BL phenotype in breast cancer cells via upregulating BRCA1-IRIS expression.

Potential transcriptional links necessary for BRCA1/p220 or BRCA1-IRIS
Using in silico analysis we compared AUF1 and pCBP2 (~1000bp upstream of +1 position) promoters in search for common factors that could link BRCA1/p220 to these genes transcription. An Oct1 binding site was common between AUF1 and pCBP2 promoters, which is interesting, since BRCA1/p220 was shown recently to induce transcription of several genes, including GADD45 through binding to Oct1 [37]. Also using in silico analysis we compared the promoter regions of these TN/BL genes (~1000bp upstream of +1 position) for common transcription binding sites, and found that all but cyclin E share an AP1 binding site. Importantly, we recently showed that BRCA1-IRIS induces cyclin D1 expression through binding to c-Jun/AP1 [32].
To study that, c-Jun was silenced in the BRCA1-IRIS-overexpressing MDAMB231 cell line (72h, Figure 5E, left upper panels) and Oct1 was silenced in the BRCA1/p220-expression HME cell line (72h, Figure 5E, right upper panels). ChIP analysis of extracts isolated from these cells confirmed that c-Jun-silencing in MDAMB231 cells significantly reduced the amounts of CK5, CK17, CH3 and EGFR promoter fragments coimmunoprecipitated by BRCA1-IRIS antibody ( Figure  5E, left lower panels), and Oct1-silencing in HME cells, dramatically reduced the amount of AUF1 and pCBP2 promoter fragments co-immunoprecipitate by BRCA1/ p220 antibody ( Figure 5E, right lower panels).
To ascertain these relationships further, CK5, EGFR, AUF1, pCBP2 promoters were cloned upstream of the luciferase gene in the pGL3 reporter plasmid. Three Sp1 binding sites cloned upstream of the luciferase gene in this reporter plasmid was used as negative control. Positive controls for BRCA1-IRIS transcription activity was cyclin D1 promoter driving luciferase reporter (see [31]), and for BRCA1/p220 induced transcription activity was GADD45 driven luciferase reporter (see [37]). The pGL3-AUF1, -pCBP2 and -GADD45, -CK5, -EGFR and -CycD1 constructs were co-transfected with GFP-, BRCA1/p220-, Oct1-, BRCA1-IRIS-or c-Jun-silenced HME (inset in Figure 5F) or MDAMB231 (not shown) cells, respectively. Luciferase activity from each reporter following the different treatments was measured in each case 72h later.
As expected Oct1 silencing significantly decreased luciferase expression from GADD45, AUF1 and pCBP2 promoters ( Figure 5F, left), but had no effect on CK5, EGFR or cyclin D1 promoters ( Figure 5F, right). Importantly, BRCA1/p220 silencing also significantly suppressed luciferase expression from GADD45, AUF1 and pCBP2 promoters ( Figure 5F, left), and as expected since BRCA1/p220 silencing upregulates BRCA1-IRIS expression, a slightly increased in luciferase expression from CK5, EGFR and cyclin D1 promoters was measured in these cells ( Figure 5F, right). On the other hand, c-Jun silencing suppressed luciferase expression from cyclin D1, CK5 and EGFR promoters ( Figure 5F, right) and more importantly, BRCA1-IRIS silencing also significantly reduced luciferase expression from these promoters ( Figure 5F, right). Luciferase expression from AUF1, pCBP2 and GADD45 promoters was not affected by c-Jun or BRCA1-IRIS silencing ( Figure 5F, left). These data show that BRCA1/p220 induces expression of AUF1 and pCBP2 through binding to and activating Oct1 on their promoters, whereas BRCA1-IRIS enhances expression of this subset of TN/BL genes by binding to and activating c-Jun on their promoters.

Loss of BRCA1/p220 enhances tumor cell survival via BRCA1-IRIS overexpression
Next, we studied whether BRCA1-IRIS overexpression in no/low BRCA1/p220 expressing cells promotes their survival and hence drug resistance phenotype associated with TN/BL breast cancers. BRCA1-IRIS and BRCA1/p220 were separately or together silenced or BRCA1-IRIS was overexpressed alone or in BRCA1/p220-silenced (i.e. BRCA1/p220 silenced in induced IRISb cells) in HME cells. Sonicated proteins were analyzed for expression and activation of AKT and its down-stream targets survivin and BAD 72h later [38,39].
Conversely, BRCA1/p220 silencing upregulated expression of BRCA1-IRIS, AKT, p-AKT, survivin and p-Bad ( Figure 6A), which led to a slight but significant increase in viability and decrease in caspase3/7 activation ( Figure 6C). BRCA1-IRIS overexpression (i.e. induced IRISb cells) had no effect on BRCA1/p220 expression, dramatically increased AKT, p-AKT, survivin ( Figure  6B, also see [34]) and p-BAD expression ( Figure 6B) and significantly increased HME and HCC1937 cells viability ( Figure 6C and D). Indeed, BRCA1/p220 silencing in induced IRISb cells (which significantly increased BRCA1-IRIS, AKT, p-AKT, survivin and p-BAD levels, Figure 6B) or BRCA1-IRIS overexpression in HCC1937 dramatically reduced the level of activated caspase 3/7, which led to increase in cell number in both cell lines above control treated cells ( Figure 6C and D). Importantly, overexpressing of wild type or clinically relevant BRCA1/p220 mutant in HCC1937 could not overcome the inhibitory effect observed with BRCA1-IRIS silencing ( Figure 6D).
Finally, colony assay was used to assess the transformation capabilities of BRCA1-IRIS overexpression or BRCA1/p220 silencing. HME cells were transfected with control or BRCA1-IRISexpressing vector, HCC1937 cells were transfected with control or BRCA1/p220-expressing vector, and MDAMB468 cells were transfected with appropriate control, BRCA1-IRIS siRNA or BRCA1/p220expressing vector. All cell lines were grown in soft agar for 2 weeks, at which time formed colonies were stained and counted. Compared to control treated cells, BRCA1-IRIS overexpression increased the number of HME colonies ( Figure 6E, left), BRCA1/ p220 overexpression reduced the number of HCC1937 colonies ( Figure 6E, middle) and BRCA1-IRIS silencing or BRCA1/p220 overexpression reduced MDAMB468 colonies ( Figure 6E, right). These data show that BRCA1-IRIS overexpression, like BRCA1/ p220 dowregulation initiates and/or maintains the transformation of mammary cells.

DISCUSSION
BRCA1-IRIS is a novel BRCA1/p220 locus produced oncogene. BRCA1/p220 inhibition or BRCA1-IRIS overexpression in mammary epithelial cells enhances expression of cyclin D1, AKT and several other proliferation and survival proteins [31- Figure 6: The effects of BRCA1-IRIS and/or BRCA1/p220 on the expression and activation of survival proteins, cell survival and transformation. Expression or activation of the indicated proteins in control, BRCA1-IRIS, BRCA1/p220 silenced (A), BRCA1-IRIS overexpressing or BRCA1-IRIS overexpressing and BRCA1/p220-silenced (B) HME cells. (C) Percentage of viable (MTT assay analysis) or dying (caspase 3/7 assay analysis) cells, respectively in HME cells treated as in A and B. Data are presented as means ± SD from triplicate, done three independent times, whereas ** is a p≤0.01. (D) Analysis of viability detected using MTT assay and cell death detected using caspase 3/7 assays in BRCA1-IRIS-silenced and/or wild type or clinically relevant mutant BRCA1/p220 overexpressing HCC1937 cells. Data are presented as the means ± SD from triplicate, done three independent times, whereas ** is a p≤0.01. (E) Soft agar analysis of control or BRCA1-IRIS overexpressing HME cells (left), control or BRCA1/p220 overexpressing HCC1937 cells (middle), or control, BRCA1-IRIS-silenced or BRCA1/p22 34,40,41, and this study]. BRCA1/p220 loss [42] or BRCA1-IRIS overexpression (ElShamy, unpublished) confers tamoxifen resistance in mammary epithelial cells. These observations support the view that BRCA1-IRIS overexpressing cells are phenotypically equivalent to no/low BRCA1/p220 expressing cells. In this study we presented a mechanistical support for this notion. We showed that, at least partially, BRCA1/p220 loss increases BRCA1-IRIS expression by a post-transcriptional mechanism, explaining the inverse relationship between the two genes.
Human and rat BRCA1/p220 mRNA 3`-UTR (~1.5kb) contains several HuR binding sites [43]. The BRCA1-IRIS 3`-UTR (~300bp from intron 11), on the other hand contains binding sites for pCBP2 [22], AUF1 and HuR [21]. It is possible that HuR stabilizes both mRNAs, and that we fail to detect an effect of HuR on BRCA1-IRIS mRNA in HME cells is because the effect is perhaps masked by the fact that these cells are BRCA1/ p220 proficient and hence express high levels of AUF1, that binds the same sites as HuR [21]. When BRCA1/p220 expression and/or function are lost and AUF1 expression is dropped (e.g., in cancer cells), BRCA1-IRIS mRNA is perhaps stabilized by HuR.
In fact, we recently performed immunohistochemical analysis of breast cancer tissue microarray (containing >300 aggressive breast cancer tumor samples) and discovered that compared to normal tissue, BRCA1-IRIS is overexpressed in the majority of these tumors, that expressed very low levels of AUF1 and pCBP2, high levels of cytoplasmic HuR and no BRCA1/p220 (ElShamy WM, unpublished data). Cytoplasmic HuR was proposed recently to be an independent prognostic factor for familial breast cancers and a poor prognosis factor for sporadic and familial breast cancers, or could even be a contributing factor to the disease [44,45]. In fact, in our recent publication [33] we showed a strong correlation between BRCA1-IRIS overexpression and cytoplasmic localization of HuR, in vitro [33].
pCBP2 binds to and activates BRCA1/p220 promoter [46]. It is possible that a positive feedback mechanism between BRCA1/p220 and pCBP2 exists, which is broken in BRCA1/p220-mutant or TN/BL cells. Further studies to elucidate the role of posttranscriptional mechanisms controlling BRCA1/p220 and BRCA1-IRIS mRNA expression in normal and breast cancer cells are required to elucidate the mechanism(s) underlying the development of familial and TN/BL breast cancers.
The inverse relationship we propose here between expression of BRCA1/p220 and BRCA1-IRIS seems not to be complete. For instance, although MCF7 and MDAMB231 cell lines express high levels of BRCA1-IRIS, both cell lines still express detectable levels of BRCA1/p220 (see above). It is possible that while intriguing, this mechanism is perhaps not the only mechanism involved. In this regard, we recently found that BRCA1-IRIS was overexpressed in xenograft or orthotopic tumors generated using HME cells overexpressing TERT/LT/Ras V12 [47]. Oncogenic Ras is known to suppress expression of several transcription factors, including vitamin D during mammary epithelial cell transformation [48]. It is possible that vitamin D is a transcription suppressor of BRCA1-IRIS. However, this remains only a hypothesis until the promoter of BRCA1-IRIS has been cloned.
Alternatively, oncogenic Ras overexpression was shown recently to decrease the expression of AUF1 during mammary cell transformation [49]. It is possible that in BRCA1/p220 expressing breast cancer tumor cells, Ras V12 instead stabilizes BRCA1-IRIS mRNA leading to its protein overexpression. However, what was even more surprising is the fact that tumors generated using HME cells overexpressing TERT/LT/BRCA1-IRIS were BRCA1/p220-negative [47]. If true also in human tumors, this suggests that BRCA1/p220 loss of expression increases BRCA1-IRIS, which in a negative feedback mechanism suppress BRCA1/p220 expression.
It is possible that patients with tumors lacking BRCA1/p220 expression or function are hit twice. Once by losing the powerful tumor suppressor, BRCA1/p220, which is involved in DNA-damage repair, cell cycle arrest, transcription and chromatin remodeling and a second time by gaining the powerful oncogene, BRCA1-IRIS, which enhances cell proliferation and survival when overexpressed. This combined effect, perhaps, contributes to increase aggressiveness and drug resistance phenotypes in no/low BRCA1/p220 expressing breast tumors, and support the view that the two proteins affect a linear pathway(s), in which BRCA1/p220 silencing and/or BRCA1-IRIS overexpression gives survival advantages to cancer cells and promotes the formation of death resistant TN/BL breast cancer cells. We therefore propose that chemotherapeutical targeting of BRCA1-IRIS might be beneficial in eradicating BRCA1/p220-associated or TN/ BL tumors cancer diseases.

Cell culture and transfection
Human mammary epithelial cells were cultured in MEGM modified medium (Lonza). All other breast cancer cell lines used in this study were grown in RPMI-1640 supplemented with 10% FBS. Small interfering RNA (siRNA) targeting BRCA1/p220 and BRCA1-IRIS were described earlier [30], whereas BARD1, AUF, pCPB2 and HuR siRNAs were from Dharmacon. Transfections of plasmids with or without siRNAs were done using oligofectamine (Invitrogen). Plasmid transfection was done using lipofectamine 2000 (Invitrogen). Cells were www.impactjournals.com/oncotarget harvested after transfection at the indicated times.

Establishment of doxycycline-induced BRCA1-IRIS expression in HME cells
Full-length BRCA1-IRIS cDNAs containing the entire 3`-UTR was amplified from HME total RNA using primers described earlier [30] was cloned in the pRevTRE plasmid (Clontech). pRev-TRE-IRIS was subsequently transfected into selected, rtTA-producing HME clones followed by selection with 150µg/ml hygromycin B (Sigma). Tet-responsive expression of BRCA1-IRIS by doxycycline (1-2 µg, Clontech) was monitored by using real time RT/PCR and/or by western analysis. The authenticity of all constructs used for transfections was verified by sequencing.

Transient infection of BRCA1-IRIS or BRCA1/ p220 cDNA
In some experiments a lentivirus expressing fulllength BRCA1/p220 or BRCA1-IRIS was used to express either protein in transient expression. Verification of expression was done using western blotting.

RT/PCR analysis
Total RNA, isolated with Trizol (Gibco, Life Technologies) and DNaseI-treated, was routinely used in RT-PCR experiments using SuperScript One-Step RT-PCR with Platinum Taq (Invitrogen). Routinely, 5 μg of total RNA or 10 μg of poly A+ RNA were used as a template in each reaction for amplification of ~450bp of BRCA1-IRIS RNA (that is, nucleotides 3,744-4,199 of BRCA1-IRIS cDNA), ~486bp of BRCA1/p220 RNA (that is, nucleotides 4,674-5,160 of BRCA1 cDNA) or ~350bp of GAPDH RNA, according to the manufacturer's instructions.
Primers to amplify CK5, CK17, CDH3, EGFR and cyclin E mRNAs are shown in Supplemental Table 1.

mRNA stability
For mRNA half-life assessments, three independent experiments were performed. Actinomycin D (5µg/ ml) was added and total RNA was prepared at the times indicated; mRNA half-lives were calculated after quantifying by RT/qPCR, normalizing to 18S RNA levels (using a 1:20 dilution of the stock sample), plotting on logarithmic scales using GraphPad Prism, and calculating the time period required for a given transcript to undergo a reduction to one-half of its initial abundance (at time zero, before adding actinomycin D) using non-linear regression analysis. Comparisons of treatment outcomes were tested for statistical differences using the Student t-test for paired data. Statistical significance was assumed at a p-value of ≤ 0.05.

Immunoprecipitation of RNP complexes
Immunoprecipitation (IP) of AUF1, pCBP2 or HuR and BRCA1-IRIS mRNA complexes from HME cell lysates was used to assess the association of the endogenous proteins with endogenous BRCA1-IRIS mRNA. The IP assay was performed essentially as described earlier [33]. Following Immunoprecipitation, extensive washes and digestion of proteins in the IP material [33], the RNA was extracted and used to perform reverse transcription (RT) followed by PCR to detect the presence of BRCA1-IRIS mRNA using gene-specific primer pairs described in [30]. GAPDH mRNA was used to normalize the data. We routinely normalized the results by measuring in parallel the binding of BRCA1-IRIS mRNA to IgG and to anti-AUF1, pCBP2 or HuR antibodies [33].