Ubiquitin-dependent regulation of COPII coat size and function

Packaging of proteins from the ER into COPII-vesicles is essential for secretion. In cells, most COPII-vesicles are ~60-80nm in diameter, yet some must increase their size to accommodate 300-400nm procollagen fibers or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII-coats have remained elusive. Here, we have identified the ubiquitin ligase Cul3Klhl12 as a regulator of COPII coat formation. Cul3Klhl12 catalyzes the monoubiquitination of the COPII-component Sec31 and drives the assembly of large COPII coats. As a result, ubiquitination by Cul3Klhl12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitination controls the size and function of a vesicle coat.

COPII-vesicles are surrounded by a coat consisting of the Sar1 GTPase, Sec23-Sec24 adaptors, and an outer layer of Sec13-Sec31 heterotetramers 12 . These coat proteins selfassemble into cuboctahedral structures with a diameter of ~60-80nm, which are too small to accommodate a procollagen fiber with a length of 300-400nm [13][14][15] . Thus, collagen transport in cells must involve factors that are absent from in vitro self-assembly reactions. Indeed, TANGO1 and its partner cTAGE5 interact with collagen and Sec23/24, thereby recruiting collagen to nascent COPII coats 16,17 . The deletion of TANGO1 in mice resulted in collagen deposition defects similar to those caused by loss of COPII 18 , and mutations in human TANGO1 are associated with premature myocardial infarction 19 . However, TANGO1 is not known to regulate the size of COPII-coats and mechanisms that permit the COPII coat to accommodate a large cargo remain poorly understood.
By analyzing mouse embryonic stem cell (mESC) division, we have identified Cul3 Klhl12 as a regulator of COPII coat formation. Cul3 Klhl12 monoubiquitinates Sec31 and drives assembly of large COPII-coats. As a result, ubiquitination by Cul3 Klhl12 is essential for collagen export, a step that is required for integrin-dependent mESC division. We conclude that monoubiquitination determines the size and function of a vesicle coat.

Cul3 regulates mESC morphology
To provide insight into stem cell-specific division networks, we depleted ubiquitination enzymes from mESCs and scored for effects on proliferation and morphology. We found that loss of the ubiquitin ligase Cul3 caused mESCs to form tightly packed cell clusters with prominent actin cables and aberrant adhesions, as seen by confocal microscopy analysis of actin and vinculin localization (Fig. 1a). A similar phenotype was observed upon depletion of UBA3, a component of the Nedd8-pathway that activates Cul3 (Fig. S1a). Cul3-depleted mESCs were delayed in proliferation (Fig. S1b, d), yet retained their pluripotency, as seen by Oct4-and alkaline phosphatase-staining and the absence of differentiation markers in expression analyses (Fig. S1c, e, f; Fig. S2b). In contrast to mESCs, depletion of Cul3 had weaker consequences in fibroblasts (Fig. 1a), although a previously reported increase in multinucleation was observed ( Fig. S1g; 20 ).
Several observations show that the mESC-phenotypes were caused by specific depletion of Cul3. First, multiple siRNAs targeting distinct regions of the Cul3-mRNA had the same effects on mESCs, with a close correlation between knockdown efficiency and strength of phenotype (Fig. S2a). Second, microarray analysis showed a strong reduction in Cul3-mRNA upon siRNA-treatment, whereas no other gene was significantly and reproducibly affected (Fig. S2b). Third, siRNAs that target closely related proteins, such as other cullins, did not disturb the morphology of mESCs (Fig. S2c). mESCs from compaction (Fig. S3a). Among several possibilities, higher RhoA-activity in the absence of Cul3 could result from RhoA stabilization or defective integrin-signaling. Stabilization of RhoA by co-depletion of all RhoA-specific Cul3-adaptors, the Bacurds 22 , did not affect mESC morphology (data not shown). By contrast, depletion of components of integrin-signaling pathways phenocopied the loss of Cul3 in mESCs (Fig. S3b); partial reduction in Cul3-levels showed synthetic lethality with dasatinib, an inhibitor of the Src kinase that acts downstream of integrin-activation (Fig. S3c); and β1-integrin was absent from the plasma membrane of Cul3-depleted mESCs (Fig. 1b).
Cul3 could regulate integrin-synthesis and trafficking, or it could allow for efficient deposition of ECM proteins to prevent integrin internalization 3 . To distinguish between these possibilities, we grew mESCs on growth factor-depleted matrigel to provide an exogenous ECM. Strikingly, under these conditions, β1-integrin was found at the plasma membrane of Cul3-depleted mESCs and no cell clustering was observed (Fig. 1b). Thus, Cul3 controls integrin-signaling in mESCs, most likely by supporting the establishment of a functional ECM.

Klhl12 is a key Cul3-adaptor in mESCs
Cul3 recruits substrates through adaptors with BTB-domains [23][24][25][26] , yet siRNA-approaches did not yield roles for BTB-proteins in ESCs. As an alternative strategy to isolate Cul3adaptors, we made use of the observation that stem cell regulators are highly expressed in ESCs, but downregulated upon differentiation 27 . Using affinity purification and mass spectrometry, we identified 31 BTB-proteins that interact with Cul3 in mESCs ( Fig. S4a; Table S1). When analyzed by qRT-PCR and immunoblot, we found that three adaptors, Klhl12, KBTBD8 and IBTK, were highly expressed in mESCs, but downregulated upon differentiation (Fig. 2a, b; Fig. S3d). Next, we depleted these adaptors from mESCs that were sensitized for changes in integrin-signaling by treatment with dasatinib. Importantly, depletion of Klhl12, but no other BTB-protein, resulted in mESC compaction, as seen with loss of Cul3 (Fig. 2c). Accordingly, endogenous Klhl12 effectively binds Cul3 in mESCs (Fig. S4b). These experiments, therefore, identify Klhl12 as a key substrate-adaptor for Cul3 in mESCs and the Cul3 Klhl12 ubiquitin ligase as an important regulator of mESC morphology.

Cul3 monoubiquitinates Sec31
To isolate the substrates of Cul3 Klhl12 , we constructed 293T cell lines that allowed for the inducible expression of FLAG Klhl12. By affinity chromatography and mass spectrometry, we identified the COPII-proteins Sec13 and Sec31 as specific binding partners of Klhl12 ( Fig.  3a; Table S2). Immunoblotting confirmed retention of endogenous Sec13 and Sec31 in Klhl12-purifications, but not in precipitates of other BTB-proteins (Fig. S5a). As seen in pulldown assays, Klhl12 directly bound Sec31, but not Sec13 (Fig. S5c, d), and this interaction was mediated the N-terminus of Sec31 (Fig. S6a) and the Kelch-domain of Klhl12 (Fig. S6b). In cells, ~30% of endogenous Klhl12 was associated with Sec13/31 ( Fig.  3b; Fig. S5b). Consistent with such a prominent interaction, Sec13/31 and Klhl12 colocalized in punctae, which likely represent ER-exit sites of COPII-vesicles ( Fig. 3c; 28 ). Importantly, siRNAs that compromise COPII resulted in mESC compaction (Fig. 3d), suggesting that Cul3 Klhl12 and the COPII-coat act in the same pathway.
In vitro, Cul3 Klhl12 catalyzed the monoubiquitination of Sec31 (Fig. 3e), which was not observed if a Klhl12-mutant with a defective Sec31-binding interface was employed (Fig.  3f). Sec31 was also monoubiquitinated in cells, which was strongly increased upon expression of Klhl12 (Fig. 3g). Klhl12-mutants unable to bind Sec31 abolished its monoubiquitination (Fig. 3h), which is likely due to dimerization with and inactivation of endogenous Klhl12 (Fig. 3a, Fig. S6c). Sec31-monoubiquitination was also strongly diminished upon expression of dominant-negative Cul3 (Fig. 3g) or depletion of Cul3 Klhl12 by siRNA (Fig. 3i). As seen upon expression of lysine-free ubiquitin, Sec31 was monoubiquitinated at one preferred and an alternative, less prominently used lysine (Fig.  3g), consistent with proteomic analyses that identified Lys647 and Lys1217 in Sec31A as ubiquitination sites 29,30 . However, neither mutation of these residues nor any other of the 65 lysine residues of Sec31 blocked ubiquitination by Cul3 Klhl12 (data not shown), revealing flexibility in the actual modification site.

Cul3 regulates the size of COPII coats
To identify a role for monoubiquitination by Cul3 Klhl12 , we induced Klhl12-expression in cells and followed the fate of Sec31 by microscopy. Shortly after Klhl12-induction, the majority of Klhl12 and Sec31 co-localized in small punctae (Fig. 4a). Over time, these punctae grew into much larger structures that contained most of Sec31, as well as other COPII-components, such as Sec13 or Sec24C (Fig. 4a, b). As seen by high-resolution confocal imaging, the large structures were hollow and spherical with a diameter of 200-500nm, and they were decorated with the proteins of the COPII-coat and with Klhl12 (Fig. 4c). Accordingly, thin-section electron microscopy revealed large, crescent-shaped tubules, possibly of ER origin, in cells transfected with Klhl12 (Fig. 4d). Immunogoldlabeling EM showed comparable structures of 200-500nm that were decorated with Klhl12 (Fig. 4d). The Klhl12-dependent structures neither contained a cis-Golgi protein; Ergic-53, which is absent from procollagen transport vesicles 31 ; ER-membrane markers that do not accumulate at ER-exit sites 32 ; nor endosomal or autophagosomal markers (Fig. S7a-c). Importantly, Sec31-binding deficient mutants, including Klhl12 FG289/290AA , neither colocalized with Sec31 nor induced formation of large structures ( Fig. 4e; Fig. S7d), and depletion of Sec31 blocked formation of large structures by Klhl12 (Fig. 4b). Thus, binding of Klhl12 to Sec31 triggers formation of large COPII-containing structures.
When Klhl12 was expressed with a Cul3-mutant that blocks Sec31-ubiquitination (Cul3 1-250 ), COPII-structures were not enlarged (Fig. 4e). In addition, depletion of Cul3 by siRNAs, which also abolishes Sec31-monoubiquitination, prevented formation of large COPII-structures by Klhl12 (Fig. 4a, c). By contrast, if Klhl12 was expressed with lysinefree ubiquitin to allow mono-, but not multiubiquitination, large COPII-structures were readily detected (Fig. 4c, e), and these structures were enriched for ubiquitin, consistent with monoubiquitination being non-proteolytic (Fig. S7e). Thus, monoubiquitination by Cul Klhl12 promotes formation of large COPII-structures, which likely represent a mixture of nascent coats at ER-exit sites and budded coats on large COPII-vesicles or tubules.

Cul3 is required for collagen export
Our screen linked Cul3 Klhl12 to the establishment of the stem cell ECM, which requires collagen secretion. Thus, the Cul3 Klhl12 -dependent increase in COPII-size might function to promote collagen export from the ER. To test this hypothesis, we expressed Klhl12 in IMR90 cells, which at steady state accumulate collagen in the ER due to inefficient export. Strikingly, Klhl12, but not Klhl12 FG289/290AA or unrelated BTB-proteins, triggered depletion of procollagen I from intracellular ER-pools (Fig. 5a). As a result, increased collagen levels were detected in the supernatant of cells expressing Klhl12, but not Klhl12 FG289/290AA (Fig. 5b). When secretion was inhibited with brefeldin A, or if collagen folding in the ER was impaired by removal of ascorbate from the medium, procollagen remained within Klhl12-expressing cells (Fig. 5a). Time-resolved experiments showed that Klhl12 strongly accelerated collagen export from IMR90 cells (Fig. 5c). Shortly after inducing secretion, Klhl12 and collagen were detected at overlapping locations ( Fig. S7f), all of which indicates that Cul3 Klhl12 facilitates collagen traffic from the ER.
Blockade of Sec31-ubiquitination by dominant-negative Cul3 interfered with the Klhl12dependent export of collagen from IMR90 cells (Fig. S8a). Similarly, depletion of Cul3 Klhl12 from engineered HT1080 fibrosarcoma cells severely impaired collagen export, and most cells retained high levels of collagen in their ER ( Fig. 5d; Fig. S8b). In contrast, smaller COPII-cargoes, such as fibronectin or EGF receptor, were properly localized in the absence of Cul3 (Fig. S8c, d). Similar observations were made in mESCs, where depletion of Cul3 led to a strong intracellular accumulation of collagen IV, comparable to the effects observed upon loss of Sec13 ( Fig. 5e; Fig. S8e). Thus, Cul3 Klhl12 is required for collagenexport, while it is less important for the trafficking of smaller COPII-cargo.
If promoting collagen export were the key role of Cul3 in mESCs, the phenotypes of Cul3depletion might be mitigated by addition of collagen in trans. Indeed, this was the case: when mESCs were plated on purified collagen-IV, depletion of Cul3 did not cause cell clustering, and β1-integrin was detected at the plasma membrane (Fig. 1c). We conclude that promoting collagen secretion is a key a function of Cul3, in agreement with its role in driving the assembly of large COPII-coats.

Discussion
In this study, we have identified Cul3 Klhl12 as an essential regulator of collagen export, which is required for mESC division. Deletion of Cul3 in mice results in early embryonic lethality with completely disorganized extraembryonic tissues 33 , a phenotype that can in part be attributed to its role in collagen secretion. Moreover, Klhl12 has been identified as an autoantigen in the connective tissue disorder Sjogren's syndrome 34 , raising the possibility that aberrant function of Cul3 Klhl12 might be related to disease. Cul3 Klhl12 monoubiquitinates Sec31 and promotes formation of large COPII-coats that can accommodate unusually shaped cargo. As a result, Cul3 is essential for the secretion of procollagen fibers, while it is not required for the transport of smaller or more flexible molecules, such as fibronectin, EGF receptor or β1-integrin. Thus, Cul3 Klhl12 appears to be specifically required for the COPII-dependent transport of large cargo.
How ubiquitination affects COPII-size or structure coats is not known. None of the 65 lysine residues of Sec31 was essential for ubiquitination by Cul3 Klhl12 , showing that Cul3 can target alternative lysine residues if the primary site is blocked. Despite this flexibility, Cul3 Klhl12 does not stoichiometrically ubiquitinate Sec31. Thus, if Sec31-ubiquitination performs a structural role, then few ubiquitinated molecules must suffice to produce large COPII-coats, and these vesicles must tolerate considerable variation in the modification site. Alternatively, as often seen with monoubiquitinated proteins, modified Sec31 might recruit an effector that delays COPII-budding or promotes coat polymerization. As Cul3 Klhl12 ubiquitinates other proteins 35 , Sec31 may not be its only substrate in the secretory pathway. Identification of the complete set of Cul3 Klhl12 -substrates and potential effector molecules should reveal the mechanism underlying the ubiquitin-dependent regulation of vesicle size.
Our findings have the potential to be translated into therapeutic strategies. We envision that agonists of Cul3 Klhl12 -function mitigate consequences of Sec23A-mutations in craniolenticulo-sutural dysplasia or Sar1-mutations in chylomicron retention disease 10,11 . By contrast, interfering with Cul3-activity may counteract increased collagen deposition during fibrosis or keloid formation 36 . Given the strong clustering phenotypes observed in Cul3depleted mESCs, inhibition of Cul3 Klhl12 might impair the proliferation of metastatic cells, which display features of undifferentiated cells 37,38 . Thus, our identification of Cul3 Klhl12 as a regulator of COPII size and function provides an exciting starting point to understand and therapeutically exploit key events in protein trafficking.

Methods summary
For stem cell culture, mouse D3 ESCs were maintained in GIBCO Dulbecco's Modified Eagle ESC medium containing 15% FBS, 1x sodium pyruvate, 1x NEAA, 1mM β-ME and 1000u/ml LIF (Millipore), and grown on gelatin-coated culture plates. Doxycyclineinducible 293T Trex FLAG BTB stable cell lines were made with Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and maintained with blasticidin and hydromycin B.
For screening, two siRNA oligos were designed against 40 mouse ubiquitin ligases (Qiagen). 10pmol of siRNA oligo and Lipofectamine 2000 were pre-incubated in a gelatin-coated 96-well plate. D3 mESCs were seeded at 15000 cells/well on top of the siRNA mixture, and the morphology of ESC colonies was examined by bright-field microscopy 48h post transfection.
To identify Cul3 Klhl12 substrates, doxycycline-inducible 239T cell lines expressing FLAG Klhl12 or FLAG Klhl9 were induced for 48h. Cleared lysate was subjected to anti-FLAG M2 affinity gel (Sigma), and precipitations were eluted with 3xFLAG peptide (Sigma). Concentrated eluates were analyzed by SDS-PAGE, and specific bands were identified by mass spectrometry analysis by the Vincent J. Coates Proteomics/Mass Spectrometry Laboratory.
For confocal microscopy, cells fixed in paraformaldehyde and permeabilized with TritonX-100 were incubated with primary antibodies for 2h and Alexa-labeled secondary antibodies (Invitrogen) for 1h. Pictures were taken on Zeiss LSM 510 and 710 confocal microscopes and analyzed with LSM image browser and Imaris 3D imaging processing software. Images were processed for contrast enhancement to remove noise.

Plasmids, protein, antibodies
Human Cul3 and Klhl12 were cloned into pcDNA4 and pcDNA5 vectors for expression in mammalian cells. Cul3, Sec31A and Sec13 were also cloned into pCS2 vector for IVT/T and expression in mammalian cells. pcDNA4-Cul3 N250 contains the first cullin repeat of the N-terminal Cul3 (1-250aa) which is sufficient for binding BTB proteins, but not Rbx1 and serves as a dominant negative for Cul3/BTB-mediated ubiquitination. The Klhl12 mutants FG289AA, RL342AA, RGL369AAA, RE416AA, YDG434AAA and RCY510AAA were made by site-directed mutagenesis.
Cul3 and Rbx1 were cloned into pFastBac, co-expressed in Sf9 ES insect cells using the Bac-to-Bac baculovirus expression system (Invitrogen) and purified as a complex by Ni-NTA agarose (Qiagen). Similarly, the Sec31A/Sec13 heterodimer and UBA1 were purified from Sf9 ES insect cells. UbcH5c and Ubc12 were cloned into pQE vector and purified from BL21(DE3) bacterial cells. Ubiquitin was cloned into pET and pCS2 vector with a Nterminal 6xHis tag. The pET-His-ubiquitin was used for bacterial purification whereas pCS2-His-ubiquitin was expressed in mammalian cells. Wildtype ubiquitin, APPBP1-UBA3 and NEDD8 were purchased from Boston Biochem.
To purify recombinant Klhl12 for ubiquitination assays, we expressed pMAL-TEV-Klhl12his and pMAL-TEV-Klhl12 FG289AA -his in BL21(DE3) cells, purified the proteins on amylose resin, cleaved them by TEV protease, and re-purified them on Ni-NTA agarose. WT-Klhl12 and mutants were also cloned into pMAL vector and purified as MBP-tagged proteins for in-vitro protein binding assays.
All shRNAs were cloned in pSuper-GFP neo vector (from Oligoengine) into BglII and XhoI sites. The GFP-Bcl2-Cb5 construct, a fusion between Bcl2 and cytochrome b5, was purchased from Clontech.
We raised mouse monoclonal antibodies against human Klhl12 and human Klhl13. Both antibodies are available at Promab Biotechnologies (cat. # 30058 and # 30067). We also raised antibodies against Sec13, Sec24C, and Sec24D. Other antibodies used in this study are: LF-67 (Anti sera for Type I procollagen) was obtained as a generous gift from Dr. Larry Fisher.

Cell culture
The D3 mouse embryonic stem cells (mESC) were maintained in ESC medium containing 15% FBS, 1x sodium pyruvate, 1x NEAA, 1mM β-ME and 1000u/ml LIF (Millipore, cat. # ESG1107) in GIBCO Dulbecco's Modified Eagle Medium, and grown on 0.1% gelatincoated tissue culture plates. Hela cells, 293T cells, 3T3 cells and IMR90 cells were maintained in DMEM plus 10% FBS. Dialyzed FBS was bought from Hyclone. The doxycycline-inducible 293T Trex Klhl12-3xFLAG stable cell line was made with Flp-In™ T-REx™ 293 Cell Line system from Invitrogen. Stable cell lines expressing other BTBproteins were generated accordingly. These cell lines were maintained with 10% TET(-) FBS, blasticidin and hydromycin B as instructed and expression was induced by 1ug/ml doxycycline.
Human lung fibroblasts IMR-90 cells were obtained from the Corielle Institute: NIA (National Institute on Aging) Aging Cell Repository. For generating procollagen stable HT-1080cell lines, we cloned proalpha(1) into a pRMc/CMV-vector and selected for neomycin resistance 39 . This vector was provided as a generous gift by Neil Bulleid. Cells were kept in a 37°C incubator at 5%CO 2 .

siRNA screen in mouse ES cells
siRNA oligos against 40 mouse ubiquitin E3 enzymes were pre-designed by Qiagen and handled as instructed. Two different siRNA oligos against each gene were included in the initial screen. 10pmol of siRNA oligos and 0.25ul of Lipofectamine2000 were pre-incubated in a 0.1% gelatin-coated 96-well plate in 20ul of OPTIMEM for 15min at room temperature. The D3 mESCs were trypsinized and seeded at 15000 cells/well in 80ul of ESC medium on top of the siRNA mixture. Fresh medium was added to the cells the next day and the morphology of ES cell colonies were examined using bright-field microscopy at 48h post transfection. Hit validation was performed with additional siRNAs that were purchased from two distinct vendors (Qiagen, Dharmacon) and that target different sites of the Cul3 mRNA. Knockdown efficiency was tested by qRT-PCR and immunoblot.

Rescue of Cul3-siRNA phenotype in mESCs by matrigel and collagen-IV
D3 mESCs were cultured on tissue culture dishes coated with gelatin (negative control), growth-factor depleted matrigel (BD Biosciences, cat# 356231), or purified collagen-IV (BD Biosciences, cat# 354233). Matrigel and collagen-IV were applied at 10μg/cm 2 . Cul3 was depleted 24h later using our standard siRNA transfection protocol, and mESC morphology was analyzed by confocal microscopy against β1-integrin, actin, and DNA.

Drug treatments of Cul3-depleted cells
To study the synthetic lethal effect of Src-inhibition with Cul3 knockdown, we treated wildtype and Cul3-depleted D3 mESCs with 0, 25, 50, 100nM of dasatinib for 18h before the phenotypes were analyzed by light microscopy.
To study the effect of Rho-inhibition on Cul3 knockdown, Cul3-depleted D3 mESCs were treated with ROCK inhibitor Y27632 at 10uM for 24h before phenotype analysis. Alternatively, RhoA was co-depleted using specific siRNAs.

Cell cycle analysis
To assess the division rate of Cul3-depleted mESCs, we treated cells with control, Cul3-, or Ube2C/Ube2S-siRNA and seeded at 3×10 5 cells/well in gelatin-coated 6-well plates. The specificity of Ube2S-and Ube2C-siRNAs was tested before 40 . The cells were trypsinized at 2, 3 and 4d post transfection and counted by hemocytometer.

ES cell differentiation analysis
To differentiate mouse ES cells into embryoid bodies (EBs), we trypsinized undifferentiated D3 mouse ES cells, washed once with LIF-free ESC media, and seeded the cells at 2×10 6 cells/dish onto 10-cm Corning Ultra-Low-Attachment Dishes (Corning cat. # 3262) containing 10 ml of ESC medium without LIF. After 24h, the cells were dissociated from the plate by gentle pipetting of the medium and collected in a 15ml Falcon tube by centrifugation. The supernatant was aspirated off and the cells were re-seeded onto 10-cm Corning Ultra-Low-Attachment Dishes containing fresh ESC medium without LIF. Medium was changed every other day for a total of 6 or 9d. Total RNA of ESCs and EB samples was extracted using TRIzol (Invitrogen, cat. # 15596-026) and chloroform. The expression of pluripotent markers and BTB genes at various time points during differentiation was analyzed using quantitative real-time PCR.
As a complementary experiment, D3 mESCs were treated with control or Oct4 siRNA. 48h after transfection, cells were collected and total RNA was extracted using TRIzol as above. The expression of pluripotent markers, tissue specific genes and BTB genes in control and Oct4-depleted cells were analyzed using qRT-PCR.

Quantitative real-time PCR analysis
We used TRIzol (Invitrogen, cat. # 15596-026) and chloroform to extract total RNA from cells. The first-strand cDNAs were synthesized by using Revertaid first strand cDNA synthesis kit (Fermentas, cat. # K1621). Gene-specific primers for qRT-PCR were designed by using NCBI Primer-Blast. The quantitative RT-PCR reaction was done with the Maxima SYBR Green/Rox qPCR system (Fermentas, cat. # K0221).

Identification of Cul3 Klhl12 -substrates
To identify Cul3 Klhl12 substrates, we generated a doxycycline-inducible hKlhl12-3xFLAG stable cell line using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen). As controls, we generated stable cell lines expressing other BTB proteins including Klhl9. Klhl12-3xFLAG and Klhl9-3xFLAG expression was induced in 30×15cm-plates by 1ug/ml of doxycycline for 48h, and cells were collected by centrifugation and lysed by douncing 40 times in PBS+0.1%NP40. The cell lysate was cleared by centrifugation and then subjected to anti-FLAG M2 affinity gel (Sigma, cat. # A2220-5mL) at 4C for 4h on a rotator. Immunoprecipitations were eluted by 300ul of 200ug/ml 3xFLAG peptide (Sigma, cat. # F4799-4MG) in PBS. The elution was repeated three times for 1h at room temperature. Eluates were pooled, concentrated to 100ul using Amicon Ultra-0.5, Ultracel-10 Membrane (Millipore, cat. # UFC501008) and run on a SDS-PAGE gel. The gel was stained by SimplyBlue™ SafeStain (Invitrogen, cat. # LC6060), and specific gel bands were cut out and sent for mass spectrometry analysis by the Vincent J. Coates Proteomics/Mass Spectrometry Laboratory at UC Berkeley.

Immunoprecipitation of endogenous protein complexes
To confirm the interaction of endogenous proteins, we lysed Hela cells or D3 mESCs by freeze-thaw twice in 20mM HEPES buffer pH7.5, 5mM KCl, 1.5mM MgCl2, 1x protease inhibitor cocktail (Roche). Specific antibodies against Cul3, Sec13 or Sec31 conjugated to protein G agarose beads were added to the cleared cell lysate and incubated at 4C for 4h. Protein complexes were eluted with gel-loading buffer at 95°C. Endogenous proteins in complexes were detected by immunoblot using specific antibodies against Cul3, Sec13, Sec31, or Klhl12.
To detect ubiquitination of endogenous COPII components, we incubated Hela cell extract with pre-immune serum or antibody against Sec13 conjugated to protein G agarose beads at 4C for 4h. Protein complexes were eluted with SDS gel-loading buffer at 95°C. Ubiquitinated proteins in the complex were detected by immunoblot against ubiquitin.

In-vitro protein interaction assays
To dissect the Klhl12 and Sec31A interaction, we coupled 20ug recombinant MBP Klhl12, various mutants or MBP as a control to 15ul amylose resin by incubating at 4C for 1h. Cul3, Sec31A and mutants were expressed from pCS2 and labeled with 35 S-Met using TnT Sp6 Quick Coupled Trsnc/trans Syst (Promega, cat. # L2080). The labeled Cul3 or Sec31A were incubated with MBP-Klhl12 or mutants at 4C for 3h. Beads were washed 4x with TBST and 2x with TBS, and incubated in SDS loading buffer at 95°C. Samples were run on SDS-PAGE and results were visualized by autoradiography.

Transmission Electron Microscopy
Mock-and Klhl12-transfected HeLa cells were grown to 70% confluence as a monolayer on an Aclar ® sheet (Electron Microscopy Sciences, Hartfield, PA). The cells were fixed for 30min in 0.1M cacodylate buffer, pH 7.2, containing 2% glutaraldehyde, and subsequently washed with buffer prior to post-fixation with 1% Osmium tetroxide on ice. This was followed by staining with 1 % aqueous Uranyl Acetate for 30 min at room temperature. For dehydration with progressive lowering of temperature, each incubation period was 10 min, with exposure to 35% ethanol at 4°C, to 50% ethanol and 70% ethanol at −20°C, and 95%, and 100% ethanol at −35°C. Cells were restored to room temperature in 100% ethanol before flat embedding in an Epon resin. Thin (70-100nm) sections were collected on Formvar-coated 200-mesh copper grids and post-stained with 2% aqueous uranyl acetate and 2% tannic acid. The sections were imaged at 120 kV using a Tecnai 12 Transmission Electron Microscope (FEI, Eindhoven, Netherlands).
For the purpose of immunolabeling, HeLa cells expressing FLAG Klhl12 or doxycyclineinducible 293T Trex FLAG Klhl12 stable cell lines were fixed in 2% paraformaldehyde and 0.5% glutaraldehyde and embedded in LR white resin. Fixation and infiltration were performed in a microwave oven (Pelco model 3450, Ted Pella, Inc., Redding, CA). 70 nm thick sections were picked on 100-mesh nickel grids coated with Formvar film and carbon, incubated in blocking buffer (5% BSA, 0.1% fish gelatin, 0.05% Tween20 in PBS) for 30 min, and followed by incubation with αFLAG antibody at a dilution of 1/40 for 1h. Goat anti-mouse IgG conjugated with 10 nm gold (BD Biosciences) was used as the secondary antibody at a diltuion of 1/40 for 1 h. Sections were poststained in 2% uranyl acetate for 5 min.

Gene expression analysis by microarray
To compare gene expression profiles of WT-mESCs versus Cul3-depleted mESCs, we transfected D3 mESCs with control or Cul3-siRNA, followed by growth on gelatin-coated 6-well plates. 48h later, total RNA was extracted by TRIzol and chloroform, and further purified using RNeasy Mini Kit (Qiagen, cat. # 74104). Microarray analysis was performed by the Functional Genomics Laboratory (UC Berkeley) using Affymetrix Mouse 430A 2.0 chip.

Analysis of collagen export from cells
IMR-90 human lung fibroblasts grown on 100mm dishes in DMEM/10% FBS were transfected with FLAG Klhl12, FLAG Klhl12 FG289AA , FLAG Keap1 and pcDNA5-flag using nucleofection kit R (bought from Lonza) as described in the manufacturer's protocol and plated on 6 well plate with 25mm coverslips. When indicated, co-transfections with 2μg each of FLAG Klhl12 and dominant-negative Cul3 were performed. Dialyzed 10% FBS media was used for ascorbate free transfections. Brefeldin A (Sigma) was used at a concentration of 2.5mg/ml and cells were incubated for 30min. MG132 was used at 20μM for 2h, choloroquine was used at 200μM for 1h. Media was collected the next day and cells on coverslips were fixed with 3% paraformaldehyde for 30min and remaining cells on a plate were used to prepare lysates. Cells on coverslips were permebealized with 0.1% Triton for 15min at room temperature followed by blocking with 1%BSA for 30min. Primary antibodies used were polyclonal anti Procollagen (LF-67,diluted 1:1000) and anti-flag (diluted 1:200). Secondary antibodies were Alexa fluor 546 donkey anti-rabbit IgG and Alexa Fluor 488 goat ant-rabbit IgG (diluted 1:200). After staining cells with appropriate primary and secondary antibodies, we fixed coverslips on slides using mounting reagent containing DAPI. Images were analyzed with a Zeiss LSM710 confocal microscope and captured with Zen10 software. Merges of images were performed with ImageJ and LSM image Browser. Media collected from 6-well plates was normalized with respect to lysate protein concentration estimated using BCA method. Media and lysates of each reaction were checked by immunoblot analysis. Tubulin was used as loading control for lysates. Ascorbate chase experiments were done by adding ascorbate (0.25mM ascorbic acid and 1mM asc-2phosphate) to Klhl12-transfected cells, followed by incubation for 5, 10, 30 and 60min.
A human fibrosarcoma cell line (HT1080) stably transfected with proalpha1(1) was used for Cul3 knockdowns. Cul3-and Klh12-shRNAs targeting two different regions in both genes were cloned into pSuperGFP and transfected using Lipofectamine 2000. pSuper GFP was used as negative control. Cells were grown on 25 mm coverslips in 6-well plates and fixed 2d post transfection. Collagen staining was done using LF-67 (1:1000) and ER was stained with anti-PDI (1:1000) antibody. Fibronectin and EGFR were stained in parallel experiments. Fibronectin expression was induced in HT1080 using 1uM dexamethasone before Cul3 knockdowns. ER retention or secretion was scored in cells expressing GFP shRNAs. Cells without GFP shRNAs and transfected with pSUPER GFP were quantified as well. Images were taken on a Zeiss LSM 710 confocal microscope and visualized with LSM image browser. Lysates were prepared from remaining cells on 6 well and checked for knockdown efficiency.   Cul3 is required for integrin-localization to the mESC plasma membrane. D3 mESCs were plated on gelatin (top two rows), growthfactor depleted matrigel, or collagen-IV. Following Cul3-depletion, cell compaction and integrin-targeting to the plasma membrane were analyzed by confocal microscopy (actin, red; β1-integrin, green; DNA, blue).  a. Immunoprecipitates of FLAG Klhl12 or FLAG Klhl9 were analyzed by Silver staining and mass spectrometry. Asterisk: non-specific band; double asterisk: breakdown product of Klhl12. b. Sec13 was immunoprecipitated from HeLa cell lysates, and Sec31 and Klhl12 were detected by immunoblot. c. Klhl12 co-localizes with COPII, as seen by confocal microscopy (Klhl12, green; Sec13, red; DNA, blue). d. D3 mESCs grown on gelatin and depleted of Sec13 were analyzed for compaction by phase (top) or confocal microscopy (actin, red; vinculin, green; DNA, blue). e. Cul3 Klhl12 monoubiquitinates Sec31. Cul3 Nedd8 -Rbx1 was incubated with Klhl12, Sec13/31, and ubiquitin or His ubiquitin. f. In vitro ubiquitination of Sec31 by Cul3 Klhl12 or Cul3 Klhl12-FG289AA was performed as above. g. Sec31 is monoubiquitinated in vivo. Upper panels: Ubiquitin conjugates were purified under denaturing conditions from MG132-treated 293T cells expressing His ubiquitin, HA Sec31, Klhl12, Cul3, or dominant-negative Cul3, and analyzed by αSec31-Western. Lower panels: the same experiment was performed with lysine-free His ubiquitin, which only allowed Sec31-monoubiquitination on at least two sites (Sec31 ubi1 and Sec31 ubi* ). h. Ubiquitin conjugates were purified from 293T cells expressing Klhl12 or Sec31-binding deficient Klhl12-mutants. i. Cul3 is essential for Sec31-ubiquitination in vivo. 293T cells were transfected with His ubiquitin and siRNAs, and ubiquitin conjugates were analyzed for Sec31 by Western.   of IMR90 cells transfected with FLAG Klhl12, or FLAG Klhl12 FG289AA was analyzed by immunoblotting. c. Collagen-I localization was analyzed IMR90 cells expressing Klhl12, after re-addition of ascorbate. d. HT1080 cells stably expressing collagen-I were transfected with shRNAs against Cul3 and analyzed by confocal microscopy (transfection control GFP, green; PDI, blue; collagen-I, red). Error bars: standard deviation, n=3. e. D3 mESCs were treated with control siRNAs or siRNAs targeting Cul3 or Sec13 and analyzed by confocal microscopy (collagen-IV, green; actin, red; DNA, blue).