Interaction of Lipocalin 2, Transferrin, and Siderophores Determines the Replicative Niche of Klebsiella pneumoniae during Pneumonia

ABSTRACT Pathogenic bacteria require iron for replication within their host. Klebsiella pneumoniae and other Gram-negative pathogens produce the prototypical siderophore enterobactin (Ent) to scavenge iron in vivo. In response, mucosal surfaces secrete lipocalin 2 (Lcn2), an innate immune protein that binds Ent to disrupt bacterial iron acquisition and promote acute inflammation during colonization. A subset of K. pneumoniae isolates attempt to evade Lcn2 by producing glycosylated Ent (Gly-Ent, salmochelin) or the alternative siderophore yersiniabactin (Ybt). However, these siderophores are not functionally equivalent and differ in their abilities to promote growth in the upper respiratory tract, lungs, and serum. To understand how Lcn2 exploits functional differences between siderophores, isogenic mutants of an Ent+ Gly-Ent+ Ybt+ K. pneumoniae strain were inoculated into Lcn2+/+ and Lcn2−/− mice, and the pattern of pneumonia was examined. Lcn2 effectively protected against the iroA ybtS mutant (Ent+ Gly-Ent− Ybt−). Lcn2+/+ mice had small foci of pneumonia, whereas Lcn2−/− mice had many bacteria in the perivascular space. The entB mutant (Ent− Ybt+ Gly-Ent−) caused moderate bronchopneumonia but did not invade the transferrin-containing perivascular space. Accordingly, transferrin blocked Ybt-dependent growth in vitro. The wild type and the iroA mutant, which both produce Ent and Ybt, had a mixed phenotype, causing a moderate bronchopneumonia in Lcn2+/+ mice and perivascular overgrowth in Lcn2−/− mice. Together, these data indicate that Lcn2, in combination with transferrin, confines K. pneumoniae to the airways and prevents invasion into tissue containing the pulmonary vasculature.

fundamental processes including cellular respiration and DNA synthesis and incorporated into heme and iron-sulfur cluster containing proteins (1,2). However, iron can be toxic by causing formation of hydroxyl radicals via the Fenton reaction. Under aerobic conditions, ferric [Fe(III)] iron is rendered insoluble through formation of oxyhydroxide polymers. To prevent these reactions, iron is generally complexed within enzymatic, carrier, and storage proteins in both humans and bacteria.
During an infection, invading bacteria must acquire iron for replication. In the mammalian host, this requires scavenging of iron from carrier proteins, such as transferrin in the blood, lactoferrin present along the mucosal surface, or heme from red blood cells (3). Some bacteria express surface receptors for these mole-cules and internalize them to access their iron. Others, including the Enterobacteriaceae family of Gram-negative bacteria, secrete iron-scavenging molecules called siderophores with extremely high affinities for iron. These siderophores can scavenge the trace amounts of free iron in the microenvironment or compete directly with mammalian carrier proteins for iron. The prototypical siderophore is enterobactin (Ent), which contains three catechol rings that coordinate a molecule of iron with the highest iron affinity of any known chelator (K d of 10 Ϫ35 M at physiological pH) (4). Pathogens may also produce siderophores in addition to Ent. Salmochelins are glycosylated forms of Ent (Gly-Ent), whose synthesis, export, and import proteins are encoded by the iroA locus (5). Yersiniabactin (Ybt) is a phenolate-type siderophore that is structurally distinct from Ent (6). This diversity of siderophores suggests specialization of function. One potential function is to evade host defenses. Indeed, Ent-dependent iron acquisition is completely disrupted by the host protein lipocalin 2 (Lcn2). Lcn2 is a cup-shaped protein containing a ligand binding pocket with high affinity for Ent. By binding Ent, Lcn2 is bacteriostatic to isolates of Escherichia coli that depend on Ent-scavenged iron for growth in serum (7,8). However, glycosylation of Ent is sufficient to prevent binding and support growth in serum and in a murine sepsis model (9). Another function of siderophores is tissue-specific iron acquisition. For example, Yersinia pestis Ybt is required to cause bubonic plague when inoculated subcutaneously but is dispensable in serum (10). However, these specialized siderophores may not be functionally equivalent to Ent. Yersiniabactin has a lower estimated affinity for iron than Ent (11). Gly-Ent is more hydrophilic than Ent and differs in its membrane partitioning and binding to serum albumin (5,12,13).
Klebsiella pneumoniae is a pathogenic member of the Enterobacteriaceae family and a leading cause of hospital-acquired pneumonia, sepsis, and urinary tract infections in the United States (14,15). This pathogen is of increasing concern due to increasing carriage of a K. pneumoniae carbapenemase (KPC), rendering some isolates resistant to almost all available antibiotics (16). In addition to its polysaccharide capsule, K. pneumoniae siderophores are known virulence factors (15). In a prospective sampling of colonizing and invasive K. pneumoniae clinical isolates, 81% encoded Ent, 17% encoded Ent and Ybt, and 2% encoded combinations of Ent, Gly-Ent, and Ybt synthesis systems (17). Ent ϩ Ybt ϩ strains were significantly overrepresented among respiratory tract isolates and KPC-encoding strains.
In a murine model of bacterial pneumonia, the severity of disease varies greatly based on the interaction of bacterial siderophores and Lcn2 (17). Consistent with its bacteriostatic effects, mice producing Lcn2 that were infected with Ent ϩ K. pneumoniae had a low lung bacterial burden, rare or no bacteria in the spleen, and no mortality. Lcn2 Ϫ/Ϫ mice had a high lung bacterial burden (Ͼ1,000 fold increase), a high spleen burden, and high mortality (Ͼ60% at 3 days). Ybt promoted growth in the presence of Lcn2, since Lcn2 ϩ/ϩ mice infected with an Ent ϩ Ybt ϩ or Ent ϩ Ybt ϩ Gly-Ent ϩ strain had a moderate bacterial burden in the lungs and spleen but low mortality (Ͻ25%). However, neither strain promoted the severe disease caused by Ent ϩ K. pneumoniae in the absence of Lcn2.
As shown for Ybt in Y. pestis, each siderophore may function only in a particular microenvironment. Therefore, differences in total bacterial burdens based on siderophore production may be due to global effects of Lcn2 or protection of certain sites within the lung. The lung is composed of distinct compartments, including the bronchial and bronchiolar airways, alveoli, interstitium, and vasculature. Infection of these sites by various bacterial and viral pathogens leads to disease patterns of bronchopneumonia, lobar pneumonia, or interstitial pneumonia that differ in severity. The host and bacterial factors that determine these patterns of pneumonia are not well understood. For K. pneumoniae, which causes bronchopneumonia and consolidation leading to severe lobar pneumonia (18,19), siderophores may determine the replicative niche of the bacteria and therefore the pattern of disease. To test this hypothesis, the pattern of infection and localization of bacteria was compared between Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice in-fected with strains of K. pneumoniae producing combinations of Ent, Ybt, and Gly-Ent.

RESULTS
Enterobactin promotes replication in the perivascular space only in the absence of Lcn2. To examine directly the severity of pneumonia in Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice, infected lungs were examined microscopically. Phosphate-buffered saline (PBS)mock-inoculated mice had normal lung architecture with little evidence of airway inflammation ( Fig. 1A and 1B) regardless of the presence or absence of Lcn2. To determine the effect of bacterial siderophores on the pattern of pneumonia, isogenic mutants of an Ent ϩ Gly-Ent ϩ Ybt ϩ K. pneumoniae strain ( Table 1) were used to infect mice. To compare the severity and localization of infection, the involvement of the airways and perivascular spaces was scored in a blinded fashion. Three days after inoculation with the iroA ybtS mutant (Ent ϩ Gly-Ent Ϫ Ybt Ϫ ), Lcn2 ϩ/ϩ mice had a mild total pathology score ( Fig. 2A) (mean ϭ 7.3) with slight airway localization ( Fig. 2B) (mean ϭ 1.3). The infection was characterized by localized areas of intense alveolar and bronchiolar inflammation consolidated into lobular/lobar pneumonia (Fig. 1A) and mild perivascular edema with some inflammatory cells (Fig. 1B). In Lcn2 Ϫ/Ϫ mice, the total pathology score was greater ( Fig. 2A) (mean ϭ 13.3) and the localization switched from airway to perivascular involvement (Fig. 2B) (mean ϭ Ϫ6.8, where a negative value denotes perivascular association). Although these mice had localized areas of alveolar and bronchiolar inflammation, there appeared to be enormous numbers of bacteria in the perivascular spaces intermixed with a moderate influx of inflammatory cells.
To confirm that these bacteria were K. pneumoniae, immunofluorescence analysis was performed. Staining of PBS mockcolonized mice demonstrated autofluorescent red blood cells in the vessel lumen but no staining in the perivascular space or lumen of the bronchioles (Fig. 3). Whereas the perivascular spaces of Lcn2 ϩ/ϩ mice contained essentially no bacteria, Lcn2 Ϫ/Ϫ mice had many intact rods that stained specifically with K. pneumoniae antiserum compared to results for the control lacking primary antibody. These data indicate that K. pneumoniae producing only Ent is able to replicate robustly in the perivascular space of Lcn2 Ϫ/Ϫ mice.
Yersiniabactin supports bacterial replication in the airways regardless of Lcn2. K. pneumoniae producing only Ybt is able to replicate to moderate numbers in Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mouse lungs (17,20). To determine how Ybt influences bacterial pneumonia, an entB mutant (Ent Ϫ Gly-Ent Ϫ Ybt ϩ ) was used to infect mice. In both Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice, the mice had a comparable, moderate pathology score ( Fig. 2A) (mean ϭ 11 and 9.3, respectively). Unlike the case of mice infected with the iroA ybtS mutant, there was little difference in the localization of infection between Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice (Fig. 2B) mean ϭ 2 and Ϫ0.5, respectively). Both sets of mice had patchy, intense inflammation in the bronchioles and dense alveolar infiltrates, consistent with bronchopneumonia. There was also variable inflammation within the perivascular spaces ( Fig. 1A and 1B). Based on immunofluorescence, there were many intact K. pneumoniae bacteria in the bronchioles and alveoli of both Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice (Fig. 3). However, there was no overgrowth of the entB mutant in the perivascular spaces of Lcn2 Ϫ/Ϫ mice. These data indicate that Ybt is sufficient to promote robust replication in the airways re-gardless of Lcn2 but cannot support growth in the perivascular space.
Lcn2 prevents invasion of the perivascular space by K. pneumoniae producing multiple siderophores. K. pneumoniae bacteria producing both Ent and Ybt, including the epidemic KPCproducing sequence type 258, are overrepresented among respiratory isolates (17). To determine how the combination of these siderophores may influence the pattern of pneumonia, an iroA mutant (Ent ϩ Gly-Ent Ϫ Ybt ϩ ) was used to infect mice. Lcn2 ϩ/ϩ mice had inflammation in both the bronchioles and alveoli ( Fig. 1A and 1B), similar to the bronchopneumonia pattern caused by the entB mutant. These mice had a moderate pathology score ( Fig. 2A) (mean ϭ 13.8) and airway localization ( Fig. 2B) (mean ϭ 7.3). Based on immunofluorescence, there were many intact bacteria within the inflamed bronchi, bronchioles, and alveoli. Few bacteria were present in the perivascular spaces relative to adjacent inflamed airways. Lcn2 Ϫ/Ϫ mice had a higher pathology score ( Fig. 2A) (mean ϭ 16.8) and a shift to perivascular localization ( Fig. 2B) (mean ϭ Ϫ8.2). There was bronchopneu-monia with intact bacteria seen by immunofluorescence throughout the airways. In addition, many bacteria were observed in the perivascular spaces of the lungs. Together, these data indicate that a strain producing both Ybt and Ent can cause pneumonia in the presence of Lcn2. However, attributable to its ability to bind Ent, Lcn2 prevents Ent-dependent replication in the perivascular space.
Some isolates of K. pneumoniae also secrete Gly-Ent, which is not bound by Lcn2. To determine the contribution of Gly-Ent to the development of pneumonia, two additional isogenic strains were used to infect mice. A ybtS mutant (Ent ϩ Gly-Ent ϩ Ybt Ϫ ) had variable pathology scores in Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice ( Fig. 2A) (means of 18 and 10, respectively). In Lcn2 ϩ/ϩ mice, the infection was more localized to the airways (Fig. 2B) (mean ϭ 1), whereas in Lcn2 Ϫ/Ϫ mice, the infection was more localized to the perivascular spaces (mean ϭ Ϫ4). The wild-type strain (Ent ϩ Gly-Ent ϩ Ybt ϩ ) caused a moderate bronchopneumonia ( Fig. 2A) (mean pathology score ϭ 7) in Lcn2 ϩ/ϩ mice with a slight airway predominance (Fig. 2B) (mean total score ϭ 2). Consistent with  the ability of Gly-Ent to promote growth in serum (8,17), there were also perivascular infiltrates that contained K. pneumoniae (Fig. 3). However, in Lcn2 Ϫ/Ϫ mice, there was more-severe infection ( Fig. 2A) (mean pathology score ϭ 16.4), with a marked increase in the amount of K. pneumoniae in the perivascular space ( Fig. 1 and 2B) (mean localization score ϭ Ϫ8). This correlates with the protection observed previously in Lcn2 ϩ/ϩ mice infected with the wild type, as measured by lung and spleen CFU (17). Therefore, Lcn2 offers partial protection during pulmonary infection, even against K. pneumoniae making multiple siderophores. The perivascular space contains serum exudate including transferrin. The observation that the iroA ybtS mutant can replicate in the perivascular space whereas the entB mutant cannot indicates nonredundant siderophore function. Increased vascular permeability is a defining component of acute inflammation, leading to leakage of protein-rich exudate from blood vessels (21). Therefore, the perivascular space likely contains blood components that inhibit growth of the entB mutant in serum (8). The iron carrier transferrin is present in large quantities and is the major source of iron in serum. To determine if the perivascular space contains transferrin as a marker of an inflammatory exudate, immunofluorescence analysis was performed (Fig. 4). Consistent with a well-controlled infection, Lcn2 ϩ/ϩ mice infected with iroA ybtS mutant K. pneumoniae showed intravascular transferrin visualized as lacy fluorescence (Fig. 4A). However, in all other infections ( Fig. 4A and 4B), transferrin was detected in the perivascular spaces. This is in contrast to the intraluminal staining of PBS-mock-colonized mice (Fig. 4D) and the autofluorescence of luminal red blood cells seen in the no-primary-antibody control (Fig. 4E). Consistent with the pattern seen by Klebsiellaspecific immunofluorescence (Fig. 3), 4=,6-diamidino-2phenylindole (DAPI)-stained bacteria were seen in transferrincontaining perivascular spaces of Lcn2 Ϫ/Ϫ mice infected with iroA mutant or iroA ybtS mutant but not entB mutant K. pneumoniae (Fig. 4C). These data, combined with the patterns of infection described above, indicate that Ent-producing K. pneumoniae can replicate in serum-containing tissue, whereas the Ybt-dependent entB mutant cannot.
Serum transferrin inhibits Ybt-dependent growth. The mechanism by which serum inhibits growth of Ybt-dependent K. pneumoniae is unclear. To test the hypothesis that Ybt has lower growth-stimulatory activity in serum than Ent and Gly-Ent, equimolar amounts of siderophore were used to stimulate growth of an entB ybtS mutant (Ent Ϫ Gly-Ent Ϫ Ybt Ϫ ). Without addition of siderophores, this mutant was significantly defective in serum growth compared to the wild type (Fig. 5A). Addition of either Ent or Gly-Ent significantly stimulated growth in serum (Fig. 5B) at 0.1 M, and both Ent and Gly-Ent restored growth to that of the wild type at 10 M. In contrast, addition of Ybt stimulated growth only at 25 M.
Next, the ability of serum to inhibit Ybt activity was tested under conditions of Ybt-dependent growth. LB medium treated with the iron chelator 2,2=-dipyridyl (DIP) was inoculated with K. pneumoniae mutants producing Ent (iroA ybtS), Ybt (entB), both (iroA), or neither siderophore (entB ybtS). The iroA and iroA ybtS mutants grew rapidly despite the addition of DIP. The entB mutant had significant growth compared to the entB ybtS mutant, attributable to endogenous production and utilization of Ybt (Fig. 6A). In contrast, all strains grew equivalently in LB alone (Fig. 6B). Addition of 1% serum had no significant effect on the entB mutant (Fig. 6C). However, addition of 10% serum significantly inhibited growth to levels comparable to that of the entB ybtS mutant (Fig. 6D). In contrast, iroA and iroA ybtS mutants grew robustly in both concentrations of serum.
To determine if transferrin is sufficient to reproduce the inhibitory effect of serum on the entB mutant, increasing amounts of of Lcn2 with enterobactin-and yersiniabactin-producing K. pneumoniae. Histological sections of lungs from Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice 3 days after retropharyngeal inoculation of 1 ϫ 10 4 CFU/ml of K. pneumoniae were scored by a pathologist for severity and extent of involvement of the airways and perivascular spaces; n Ն 4 mice per group. The mean total (A) or localization (B) score is shown for each mouse genotype infected with wild-type (WT) or mutant K. pneumoniae producing the siderophores indicated (ϩ). A positive localization score indicates airway predominance; a negative score indicates perivascular predominance. purified human transferrin were added ( Fig. 6E and 6F). Transferrin added at 2.5 mg/ml, which is within the physiologic concentration range of human serum (2 to 4 mg/ml) (22), inhibited the entB mutant but not the iroA or iroA ybtS mutant. However, addition of Lcn2 significantly inhibited growth of both the iroA and iroA ybtS mutants (Fig. 6G). Therefore, the combination of Lcn2 and transferrin-containing exudate could protect the perivascular space from K. pneumoniae producing Ent and/or Ybt.

DISCUSSION
The pattern of pneumonia, as visualized by radiographic or histological analysis, correlates with the etiologic agent causing the disease. Bacterial infection may present as aspiration pneumonia, bronchopneumonia, or lobar pneumonia. In contrast, viral and mycoplasma infections typically demonstrate an interstitial pattern (21,23). This study, comparing the pathology caused by K. pneumoniae bacteria producing various siderophores, indicates that the pattern of bacterial pneumonia is influenced by the ability of the pathogen to acquire iron. Ent ϩ K. pneumoniae represents the most common phenotype in our collection of clinical isolates (17). Representing this phenotype, the iroA ybtS mutant causes limited, multifocal pneumonia consistent with inoculation by aspiration. Ent ϩ Ybt ϩ K. pneumoniae bacteria are a significant subset of clinical respiratory isolates, including KPC-producing strains. The Ent ϩ Ybt ϩ iroA mutant elicits an infiltrate of neutrophils in the bronchi, bronchioles, and adjacent airways, consistent with bronchopneumonia. Infection with this type of K. pneumoniae can also progress to larger areas of consolidation in the lung.
The difference in lung pathology between Ent ϩ and Ent ϩ Ybt ϩ K. pneumoniae is dependent on the interaction of siderophores and Lcn2. Ent is counteracted by Lcn2, which effectively contains the infection to the site of inoculation. In contrast, Ybt is able to support replication in the presence of Lcn2, leading to greater lung pathology (Fig. 2) and greater density of bacteria in the lung (17).
In the absence of Lcn2, both types of K. pneumoniae cause a severe pneumonia with a distinct pattern of inflammation and infection around blood vessels. Recently, Ybt has been shown to bind copper in competition with iron during E. coli urinary tract infections and to prevent copper toxicity (24). Therefore, the effects of Ybt on K. pneumoniae growth may be attributable to copper binding, iron binding, or both. Preferential binding of Ybt to copper over iron may explain why exogenous Ybt can support growth of K. pneumoniae in serum but only at concentrations Ͼ100-fold higher than the effective dose of Ent or Gly-Ent. Similarly, Ybt may be unable to scavenge sufficient iron for growth in the presence of competing copper and transferrin, despite promoting growth on transferrin in isolation (11,25). If the primary function of Ybt is to bind copper, then the inhibitory effect of serum and transferrin on the entB mutant may be due to inhibition of siderophoreindependent iron acquisition rather than to Ybt itself. Because Ent can enhance copper toxicity (24), the iroA ybtS mutant may be more susceptible to copper toxicity than the iroA and entB mutants, contributing to its profound inhibition in Lcn2 ϩ/ϩ mice. In turn, the moderate pneumonia caused by the entB mutant may be enabled by prevention of copper toxicity instead of enhanced iron acquisition. However, the iroA and iroA ybtS mutants both cause severe disease in Lcn2 Ϫ/Ϫ mice, suggesting that the copper binding effect of Ybt is dispensable under certain conditions. show immunofluorescence staining of formalin-fixed paraffin-embedded histological sections of lungs from Lcn2 ϩ/ϩ and Lcn2 Ϫ/Ϫ mice 3 days after retropharyngeal inoculation of 1 ϫ 10 4 CFU/ml of WT or mutant K. pneumoniae encoding the siderophores indicated (ϩ), using rabbit anti-Klebsiella serum and Cy3-conjugated goat anti-rabbit secondary antibody (red) and the nucleic acid stain DAPI (blue), except for "No 1°," in which anti-Klebsiella serum was omitted. Vessels (V) and bronchioles (B) are shown; magnification, ϫ200.
Ent and Ybt differ in their ability to support growth within niches of the lung. When unimpeded by Lcn2, Ent can support growth in the airways and perivascular spaces of the lung. In contrast, the entB mutant is confined to the airways and does not promote growth in the perivascular spaces. This defect is attributable to the inability of Ybt to promote growth in the presence of serum transferrin. In contrast, lactoferrin did not have a consistent effect on growth in vitro (data not shown).
The inability of Ybt to support growth in serum indicates that the iron chelating ability of inflammatory exudate compensates for gaps in Lcn2 siderophore specificity. In the presence of Lcn2, changes in vascular permeability may allow efflux of inflammatory cells without providing an accessible iron source for bacteria. Specifically, Lcn2 inhibits Ent and serum transferrin inhibits Ybtdependent growth, either directly or indirectly. In the absence of Lcn2, all Ent-producing strains can invade the perivascular spaces. This leads to overwhelming bacterial growth in these spaces (Fig. 3) and corresponds with dissemination to the spleen and systemic illness (17).
The role of Gly-Ent (salmochelin) during K. pneumoniae lung infection remains unclear. In E. coli, production of Gly-Ent is sufficient to promote pulmonary infection in an avian air sac model (26). Accordingly, the ability of K. pneumoniae to produce Gly-Ent (ybtS mutant) compared to Ent alone (iroA ybtS mutant) correlates with worsened disease. Perhaps because only a portion of Ent is glycosylated (8,17), the ybtS mutant caused infections with various overall severities (Fig. 2). In Lcn2 ϩ/ϩ mice, the ability to produce Gly-Ent did not appear to worsen the infection compared to findings for mutants that produce Ybt. Despite their ability to grow in mouse serum containing Lcn2 (8), both Gly-Entproducing strains primarily infected the airways of Lcn2 ϩ/ϩ mice and switched to perivascular involvement in Lcn2 Ϫ/Ϫ mice. Furthermore, Lcn2 significantly inhibited bacterial density of the wild type (Ent ϩ Gly-Ent ϩ Ybt ϩ ) (17). Ent may be more efficient than  Gly-Ent at acquiring iron in the perivascular spaces, or the ability of Lcn2 to sequester Ent as a significant proportion of the total siderophore pool may account for its partial protection against the wild type that produces three siderophores.
In summary, this work demonstrates that the interaction between Lcn2, transferrin, and bacterial siderophores determines the replicative niche of K. pneumoniae in the lung. Transferrin is an efficient barrier against Ybt-dependent bacterial growth. Lcn2 is sufficient to inhibit the progression of pneumonia caused by K. pneumoniae producing only Ent, comprising the majority of isolates. Importantly, Lcn2 protects the perivascular space from a virulent subset of strains that produce multiple siderophores. Defense of the perivascular space is crucial, since invasion leads to intense bacterial growth, systemic spread, and potentially fatal disease.

MATERIALS AND METHODS
Bacterial strains and media. All K. pneumoniae strains used are rifampinresistant derivatives of ATCC 43816, and their construction has been described previously (Table 1). K. pneumoniae was cultured in Luria-Bertani broth with agitation at 37°C or agar at 30°C overnight, supplemented with rifampin (30 g/ml; all strains) and kanamycin (50 g/ml; KP20 and KP25).
Murine pneumonia model. All animal studies were approved by the University of Pennsylvania Institutional Animal Care and Use Committee. Lcn2 Ϫ/Ϫ mice (7) that had been backcrossed with C57BL/6 mice or their wild-type counterparts (Lcn2 ϩ/ϩ ) were used. Infections were carried out as previously described (17). Briefly, overnight cultures of K. pneumoniae were collected by centrifugation and diluted in phosphatebuffered saline, and 1 ϫ 10 4 CFU was administered in 50 l of saline into the pharynx of mice under isoflurane anesthesia. Three days after infection, mice were sacrificed by CO 2 asphyxiation. For histological examination, lungs were perfused with 10% normal buffered formalin (Sigma) via tracheal instillation and ligation, paraffin embedded, sectioned, and stained with hematoxylin and eosin (H&E).
Scoring of lung pathology. Histological sections were scored by a pulmonary pathologist in a blinded fashion, using a modified version of the system of Stapleton et al. (27). An airway severity score was assigned as follows: 0, no involvement; 1, localized infiltrates of neutrophils within the alveolar sacs with increased alveolar macrophages, no bacteria seen; 2, dense infiltrates of neutrophils in bronchioles and larger airways with involvement of adjacent alveoli, no bacteria seen; 3, consolidation of neutrophil inflammation in bronchioles and adjacent alveoli, lobular or lobar pneumonia, or intact visible bacteria seen at magnification ϫ40 but inflammatory cells greater than bacteria; 4, overwhelming infection with bacteria greater than inflammatory cells in alveoli or bronchioles. A perivascular severity score was assigned as follows: 0, no involvement; 1, mild perivascular infiltrates without involvement of the peribronchiolar regions, no bacteria seen; 2, moderate perivascular and peribronchiolar infiltrates, no bacteria seen; 3, severe perivascular and peribronchiolar infiltrates or intact visible bacteria seen at magnification ϫ40 but inflammatory cells greater than bacteria; 4, overwhelming infection with bacteria greater than inflammatory cells in perivascular and peribronchiolar regions. A modifier score for severity was determined for both airway and perivascular sites on the following scale: 0, no inflammation or infection seen;1, 1 to 10% involvement by either inflammatory cells or intact bacteria; 2, 11 to 20% involvement by either inflammatory cells or intact bacteria; 3, 21 to 30% involvement by either inflammatory cells or intact bacteria; 4, Ͼ30 percentage point involvement by either inflammatory cells or intact bacteria. For each mouse, a total score was calculated as (airway score ϫ airway modifier) ϩ (perivascular score ϫ perivascular modifier) and a localization score was calculated as (airway score ϫ airway modifier) Ϫ (perivascular score ϫ perivascular modifier).
Immunofluorescence microscopy. Slides with paraffin sections were warmed to 60°C, and paraffin was removed by stepwise treatment with xylene (twice, 4 min each), 100% ethanol (twice, 2 min each), and 1 min each of 95%, 90%, 80%, and 70% ethanol. Slides were then rinsed in water. For Klebsiella immunofluorescence, antigen retrieval was performed in Tris-EDTA buffer (Tris base, 1.2 g/liter; EDTA, 0.37 g/liter), pH 8, by microwaving for 15 min and cooling for 15 min (28). Slides were washed in saline and treated with starting block reagent for 10 min. The primary antibody was diluted in saline containing 0.1% bovine serum Final OD 600 s between strains were compared using one-way ANOVA with Tukey's multiple-comparison test (*, P Ͻ 0.05; **, P Ͻ 0.01; ns, not significant). albumin and 0.2% Triton X-100 and incubated on the slides overnight at 4°C. Rabbit anti-Klebsiella serum (United States Biologicals) was used at 1:4,000; rabbit anti-mouse transferrin (R&D Systems) was used at 1:50. Slides were washed in PBS and stained with Cy3-conjugated donkey antirabbit antibody diluted 1:600 in PBS with albumin and Triton X-100 for 30 min at 37°C. Slides were washed, stained with a 1:10,000 dilution of DAPI in water for 20 s, and washed in water, and coverslips were mounted with Kirkegaard & Perry mounting medium.
In vitro growth assays. Iron-limited medium was prepared by supplementing Luria-Bertani broth with the iron chelator 2,2=-dipyridyl (Acros) to a final concentration of 350 M. Where indicated, increasing concentrations of human serum, partially saturated human transferrin (Sigma), and recombinant human Lcn2 were added. Purification of Lcn2 was performed as previously described (17). Overnight cultures of K. pneumoniae were diluted 1:10,000 in saline, and 10 l was added to 100 l of iron-limited broth with or without supplements. Samples were incubated overnight in 96-well plates at 37°C. To assay the effect of exogenous siderophores, dilutions of K. pneumoniae were prepared and incubated as above in RPMI medium (Invitrogen) containing 10% human serum and increasing concentrations of the purified siderophores enterobactin, yersiniabactin, and salmochelin S4 (EMC microcollections). To measure bacterial density, samples were serially diluted in saline and plated on agar with appropriate antibiotics.