Structure-guided Mutational Analysis of the OB, HhH, and BRCT Domains of Escherichia coli DNA Ligase*

NAD+-dependent DNA ligases (LigAs) are ubiquitous in bacteria and essential for growth. LigA enzymes have a modular structure in which a central catalytic core composed of nucleotidyltransferase and oligonucleotide-binding (OB) domains is linked via a tetracysteine zinc finger to distal helix-hairpin-helix (HhH) and BRCT (BRCA1-like C-terminal) domains. The OB and HhH domains contribute prominently to the protein clamp formed by LigA around nicked duplex DNA. Here we conducted a structure-function analysis of the OB and HhH domains of Escherichia coli LigA by alanine scanning and conservative substitutions, entailing 43 mutations at 22 amino acids. We thereby identified essential functional groups in the OB domain that engage the DNA phosphodiester backbone flanking the nick (Arg333); penetrate the minor grove and distort the nick (Val383 and Ile384); or stabilize the OB fold (Arg379). The essential constituents of the HhH domain include: four glycines (Gly455, Gly489, Gly521, Gly553), which bind the phosphate backbone across the minor groove at the outer margins of the LigA-DNA interface; Arg487, which penetrates the minor groove at the outer margin on the 3 ®-OH side of the nick; and Arg446, which promotes protein clamp formation via contacts to the nucleotidyltransferase domain. We find that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation.

Escherichia coli NAD ϩ -dependent DNA ligase (LigA) 2 exemplifies a family of essential DNA replication/repair enzymes found in all bacteria. LigA catalyzes the sealing of 3Ј-OH/5Ј-PO 4 nicks in duplex DNA via a series of three nucleotidyl transfer reactions. (i) LigA reacts with NAD ϩ in the absence of nucleic acid to form a covalent ligase-(lysyl-N-)-AMP intermediate and release NMN; (ii) LigA-AMP binds to nicked duplex DNA and transfers the adenylate from the active site lysine to the 5Ј-PO 4 terminus to form an adenylylated nicked intermediate, AppDNA; (iii) LigA remains bound to the adenylylated nick and immediately catalyzes the attack of the nick 3Ј-OH on the 5Ј-phosphoanhydride linkage, resulting in a repaired phosphodiester and release of AMP (1-3).
Domain Ia is unique to NAD ϩ -dependent ligases and is the determinant of NAD ϩ specificity (9 -11). During the ligase adenylylation reaction, the Ia domain closes over the NAD ϩ nucleotide bound by the NTase domain, grabs the NMN moiety of NAD ϩ via multiple contacts to essential amino acids in domain Ia, and thereby orients the NMN leaving group apical to the attacking lysine nucleophile (Lys 115 in E. coli LigA) (6,10). Recognition of the AMP moiety of NAD ϩ and the catalysis of nucleotidyl transfer chemistry is accomplished by a constellation of essential amino acid side chains within the NTase domain that contact the adenine base, the ribose sugar, or the ␣-phosphate of the adenylate (5,6,8,12,13) (Fig. 1). Most of the essential NTase residues are located within a set of five peptide motifs that define a covalent nucleotidyltransferase superfamily, which includes ATP-dependent DNA ligases, ATP-dependent RNA ligases, and GTP-dependent mRNA capping enzymes (14). An N-terminal fragment of LigA, comprising just the Ia and NTase domains, is competent to catalyze ligase adenylylation (9,(15)(16)(17)(18), but it is unable to perform the second and third steps of the pathway. Deleting only the Ia domain from NAD ϩ -dependent ligases abolishes ligase adenylylation without affecting phosphodiester formation at a preadenylylated nick (step 3) (9 -11). These results, and others (9,15,16,18,19), implicate the C-terminal domains of NAD ϩdependent ligases in recognition of the DNA substrate.
The crystal structure of E. coli LigA bound to the nicked DNA-adenylate intermediate (8) revealed that LigA encircles the DNA helix as a C-shaped protein clamp (Fig. 1). The protein-DNA interface entails extensive DNA contacts by the NTase, OB, and HhH domains over a 19-bp segment of duplex DNA centered about the nick (Fig. 1). The NTase domain binds to the broken DNA strands at and flanking the nick, the OB domain contacts the continuous template strand surrounding the nick, and the HhH domain binds both strands across the minor groove at the periphery of the footprint. The zinc finger module bridges the OB and HhH domains, and it contributes only a single main-chain amide contact to the DNA backbone (8). Domain Ia makes no contacts to the DNA duplex, consist-ent with its dispensability for catalysis of strand closure on an AppDNA substrate (10). No electron density was observed for the C-terminal BRCT domain, notwithstanding that the crystals contained full-length LigA and there was empty space in the lattice adjacent to the HhH domain (8). There is a lack of consensus in the literature concerning the necessity and function of the BRCT domain of NAD ϩ -dependent DNA ligases. The fact that a second E. coli LigA paralog (LigB) and two entomopoxvirus ligases have NAD ϩ -dependent nick sealing activity, although they lack the BRCT domain (9,20,21), indicates that this structural module is not a defining requirement for NAD ϩ -dependent ligation. However, there have been several reports of the effects of deleting the BRCT domain of bacterial or viral LigA proteins, which range from deleterious (11,22,23) to mild or minimal (17)(18)(19), depending on which LigA enzyme is being investigated.
Here, we used the E. coli LigA-DNA crystal structure to guide a mutational analysis of the constituents of the OB and HhH domains that either make atomic contacts to the DNA substrate or are implicated in the formation of the ligase clamp. We thereby defined a suite of amino acid functional groups, distant from the LigA active site, that are essential for nick sealing. We also conducted a deletion analysis and alanine scan of the BRCT domain of E. coli LigA guided by an NMR structure of the BRCT domain of Thermus thermophilus LigA. 3

EXPERIMENTAL PROCEDURES
Ligase Mutants-Missense and nonsense mutations were introduced by PCR into the pET-EcoLigA expression plasmid as described previously (12). The entire ligA gene was sequenced in every case to confirm the desired mutation and exclude the acquisition of unwanted changes during PCR amplification and cloning. The expression plasmids were transformed into E. coli BL21(DE3). Mutant and wild-type ligases were purified from the soluble lysates of isopropyl-1-thio-␤-Dgalactopyranoside-induced BL21(DE3) cells by nickel-agarose chromatography as described (12). The protein concentrations were determined using the Bio-Rad dye reagent with bovine serum albumin as a standard.
Nick Ligation-Reaction mixtures (20 l) containing 50 mM Tris-HCl (pH 7.5), 10 mM (NH 4 ) 2 SO 4 , 5 mM dithiothreitol, 5 mM MgCl 2 , 20 M NAD ϩ , 1 pmol of 5Ј 32 P-labeled nicked duplex DNA substrate, and aliquots of serial 2-fold dilutions of wild-type or mutant ligases were incubated at 22°C for 20 min. The products were resolved by electrophoresis through a 15-cm 18% polyacrylamide gel containing 7 M urea in 0.5ϫ TBE (45 mM Tris borate, 1.25 mM EDTA). The extents of ligation were determined by scanning the gel with a Fujix BAS2500 imager. The specific activities of wild-type and mutant ligases were determined from the slopes of the titration curves in the linear range of enzyme dependence. The activities of the mutant ligases were normalized to the specific activity of wildtype LigA protein purified in parallel with that mutant and assayed in a parallel with the same preparation of radiolabeled DNA substrate. On average, the wild-type LigA sealed 26 Ϯ 4.9 fmol of nicks per fmol of input enzyme (average value for 10 different titration experiments with three different preparations of recombinant wild-type LigA).

RESULTS AND DISCUSSION
Alanine Scanning of DNA-binding Residues of the LigA OB Domain-The OB domain of E. coli LigA comprises a fivestrand antiparallel ␤ barrel plus an ␣ helix (8). The concave surface of the OB barrel nestles up to close to the backbone of the template strand ( Fig. 2A), donating hydrogen bonds to the phosphodiester oxygens from the Gln 330 , Arg 333 , Thr 334 , and Asn 355 side chains and the main-chain amides of residues 334, 352, 354, and 355. The template strand interface of the OB domain spans six consecutive phosphates centered about the nick ( Figs. 1 and 2A).
The OB domain makes additional contacts with the DNA in the minor groove opposite the adenylylated nick (Fig. 2B). Val 383 and Ile 384 project into the minor groove and make van der Waals interactions with the terminal cytosine bases on either side of the nick and the penultimate pentose sugar on the 5Ј side of the nick. Arg 379 makes water-mediated contacts in the minor groove to the base and sugar of the two template strand nucleosides flanking the 3Ј-OH at the nick. Gln 386 makes a water-mediated contact to the 5Ј-PO 4 strand (Fig. 2B).
To probe whether any of the aforementioned side chain contacts to DNA are functionally relevant, we introduced single-alanine substitutions for Gln 330 , Arg 342 , Asn 355 , Arg 379 , and Gln 386 . Double-alanine changes were introduced at vicinal residues Arg 333 -Thr 334 and Val 383 -Ile 384 . The wild-type and mutated E. coli LigA proteins were produced as N-terminal His 10 fusions and purified in parallel from soluble bacterial extracts by nickel-agarose chromatography (Fig. 3A, left panel). The extent of ligation of singly nicked 3Ј-OH/5ЈPO 4 DNA by wild-type LigA and each mutant was gauged as a function of input enzyme, and the specific activities were normalized to the wild-type value (defined as 100%). Our operational definition of an important amino acid (or dipeptide) was one at which alanine substitution reduced nick sealing activity to Յ10% of the wild-type level. By this criterion, the Arg 333 -Thr 334 dipeptide, Arg 379 , and the Val 383 -Ile 384 dipeptide were deemed essential for ligation ( Fig. 3B, left panel). An alignment of the primary structures of the OB domains of LigA enzymes from various bacterial genera (including Haemophilus, Mycobacterium, Bacillus, Staphylococcus, Enterococcus, and Thermus) and eukaryal DNA viruses (mimivirus and entomopoxvirus) reveals that the Arg 333 , Arg 379 , Val 383 , and Ile 384 side chains are conserved in all of the LigA proteins (Fig. 2C). Thr 334 is conserved in the LigA enzymes from bacteria, with the exception of Thermus filiformis (Fig. 2C).
By contrast, the alanine mutation at Gln 330 (which is not conserved in the LigA family; Fig. 2C) had no apparent effect on nick sealing, signifying that the bifurcated direct and water-mediated contacts of the Gln 330 amide group to two of the phosphates of the template DNA strand ( Fig. 2A) are not important for activity. The Q386A mutant had one-third the activity of wild-type LigA (Fig. 3B); thus, the water-mediated contact from Gln N⑀ to the phosphodiester backbone ( Fig. 2B) might contribute modestly, although not enough to meet our criterion of significance. In addition to this DNA contact, the Gln 386 O⑀ engages in a hydrogen bond with the Arg 379 side chain emanating from the neighboring ␤ strand (not shown); loss of this contact might contribute to the activity decrement of the Q386A mutant. Gln 386 is conserved as glutamine or glutamate in all of the bacterial LigA proteins but is replaced by tyrosine or isoleucine in the viral LigA clade (Fig. 2C).
Eliminating Asn 355 and its amide hydrogen bond to a phosphate on the template strand ( Fig. 2A) reduced nick sealing to one-third the wild-type level (Fig. 3B). It is conceivable that the effects of the N355A change are modest because the same phosphate receives functionally redundant hydrogen bonds from the main-chain amides of Asn 355 and its neighbor Ser 354 ( Fig.  2A). It is also possible that the 3-fold activity decrement of the N355A mutant reflects the loss of the hydrogen bond interaction with Arg 342 in the neighboring strand of the ␤ sheet ( Fig.  2A), rather than loss of phosphate contacts. Arg 342 itself participates in the same water-mediated phosphate contact as Gln 330 O⑀ ( Fig. 2A). The 5-fold decrement in nick sealing caused by the R342A change (Fig. 3B) better mimics the effects of N355A than Q330A, which implies that the role of Arg 342 might be to stabilize the OB ␤ sheet (via its contacts to Asn 355 on one side and Glu 328 on the other; Fig. 2A). The Arg 342 -Asn 355 pair is confined to E. coli LigA (Fig. 2C); a similar Lys-Asn pair is present in Haemophilus influenzae LigA, but all other aligned LigA enzymes have diverse substitutions at the equivalent positions.
Structure-Activity Relationships at Key Residues of the OB Domain-To refine the structure-function map of the OB domain, we introduced single-alanine changes at the essential Arg 333 -Thr 334 dipeptide (Fig. 4A). The specific activities of R333A and T334A were 0.8 and 21% of wild-type LigA, respectively (Fig.  4B). Thus, Arg 333 is essential by our criterion, but Thr 334 is not. The Thr 334 O␥ donates a hydrogen bond to a nonbridging phosphate oxygen atom of the same phosphodiester coordinated by Arg 333 (which engages the other nonbridging oxygen). The substantial residual activity of T334A could reflect functional redundancy of the missing O␥ hydrogen bond with the hydrogen bond donated to the same phosphate oxygen by the Thr 334 mainchain amide (Fig. 2A).
The essential Arg 333 side chain was substituted conservatively by lysine and glutamine (Fig. 4A). The finding that lysine resulted in significant gain of activity (to 22% of wild type), whereas glutamine did not (0.8%), attests to the importance of positive charge and the ionic interactions with the phosphate backbone seen in the crystal structure (Fig. 2). The eukaryal viral LigA proteins naturally have lysine in place of the Arg 333 side chain conserved among bacterial LigA proteins (Fig. 2C).
The OB fold Arg 379 residue defined as essential by the initial alanine scan was also replaced conservatively by lysine and glutamine (Fig. 4A). Although lysine afforded some recovery of function when compared with the R379A mutant (6.3% versus 0.7% of wild type, respectively), the R379K protein still satisfied our criterion for a significant mutational impairment. Glutamine (1.2% activity) had no salutary effect. We surmise that positive charge at position 379 does not suffice for nick sealing activity, which depends specifically on arginine, a residue that can form multidentate hydrogen bond contacts. Indeed, the crystal structure shows that Arg 379 not only makes a water-mediated contact to adjacent nucleosides of the template strand ( Figs. 1 and 2B), it donates hydrogen bonds from its terminal guanidinium nitrogens to main-chain carbonyls of Ala 349 and Val 383 (Fig. 2B). Ala 349 is located at the tip of the hairpin turn linking two of the flanking ␤ strands of the OB barrel that pack tightly against the DNA (Fig. 2B). We infer that Arg 379 is needed to stabilize the OB fold in a proper conformation for productive DNA binding. Arg 379 is conserved in bacterial LigA proteins but is replaced by lysine in the viral homologs (Fig. 2C).
The mutational effects at Val 383 and Ile 384 support their imputed role in deforming the local structure of the DNA duplex surrounding the nick (8). The two base pairs flanking the nick on the 3Ј side and the base pair at the nick 5Ј terminus adopt an RNA-like A helical conformation. Also, the inclination and twist angles between adjacent base pairs exhibit an abrupt distortion at the nick termini, which results in bending and unwinding of the double helix and widening of the major groove surrounding the nick. The contacts of Val 383 and Ile 384 with the nucleosides in the minor groove at the nick were proposed to be a principal factor in splaying apart the bases (8). The present mutational data suggest that this OB-induced distortion is a necessary component of the ligation pathway and are in accord with findings that Thermus LigA is sensitive to perturbations of the nick 3Ј-OH base pair in the minor groove (24).

Effects of Mutations at DNAbinding Residues of the LigA HhH
Domain-The HhH domain of E. coli LigA anchors the phosphodiester backbone at four points that are arrayed symmetrically with respect to the nick (Fig. 5A). The LigA HhH domain consists of two tandem (HhH) 2 modules, each composed of five ␣ helices, arranged with pseudo two-fold symmetry in the LigA structure. The four-point binding of the HhH domain at the periphery of the LigA-DNA footprint stabilizes a 10°DNA bend centered at the nick. The functional DNA binding units of the E. coli LigA HhH domain are the four "loop-helix" motifs: 452 DGMGD-KI 458 , 487 RMGPKS 492 , 519 EVGEA-T 524 , and 551 DVGIVV 556 . Each binds to a NpNpN trinucleotide via a network of hydrogen bonds from backbone amide nitrogens to the phosphate oxygens of the NpNpN element. The proximal phosphate (5Ј-NpNpN) fits into an oxyanion hole formed by the amino acids at the N terminus of the ␣ helix, which lacks a helix-capping side chain because each helix initiates with a conserved glycine (Gly 455 , Gly 489 , Gly 521 , or Gly 553 ) that contributes to the oxyanion hole (Fig. 1). The adjacent phosphate (5Ј-NpNpN) receives a hydrogen bond from the backbone amide of a hydrophilic amino acid in the loop located two or three residues upstream of the conserved glycine. In E. coli LigA, these polar side chains (Asp 452 , Arg 487 , Glu 519 , or Asp 551 ) straddle or penetrate into the DNA minor groove. In particular, Arg 487 projects deeply into the minor groove, where it makes multiple direct and water-mediated hydrogen bonding contacts to the bases and the sugar atoms of both DNA strands (Fig. 1).
To assess whether the oxyanion holes are functionally important, we introduced the aliphatic chains Ala and Val in lieu of Gly 455 , Gly 489 , Gly 521 , and Gly 553 . We also replaced the glycines with aspartates, thereby creating the potential for electrostatic repulsion between each of the HhH loop-helix motifs and the DNA phosphodiester backbone. The 12 Gly-to-Ala/ Val/Asp mutants of LigA were purified (Fig. 3A) and then tested for nick sealing in parallel with wild-type LigA (Fig. 3B). In the 452 DGMGDKI 458 loop-helix motif that contacts the 3Ј-OH DNA strand (Fig. 1), changing Gly 455 to alanine was well tolerated (44% activity), but nick sealing was suppressed 100-fold by valine or aspartate. Modeling a valine or aspartate side chain into the LigA-DNA crystal structure (without modifying the position of the polypeptide main chain) reveals a severe steric clash between the valine or aspartate side chain and the phosphate in the oxyanion hole (not shown). Although alanine would also impinge on the phosphate when modeled into the crystal structure (albeit less so than valine or aspartate), the biochemical data show that one extra methyl group can be accommodated without much effect on sealing, perhaps by a compensatory local adjustment in the main chain of the loop in the loophelix motif.
The 487 RMGPKS 492 motif contacts the template strand on the 3Ј-OH side of the nick (Fig. 1). We found that the G489A protein retained one-fourth of wild-type activity, whereas G489V and G489D had 3.2 and 0.8% of wild-type activity, respectively (Fig. 3B). The 551 DVGIVV 556 motif binds to the 5Ј-PO 4 DNA strand (Fig. 1). Although G553A had 38% activity, G553V and G553D were reduced to 0.9 and 2.6% activity, respectively (Fig. 3B). The loop-helix motif 519 EVGEAT 524 , which binds to the template DNA strand on the 5Ј-PO 4 side of the nick (Fig. 1), was uniquely sensitive to alanine substitution at the glycine of the oxyanion hole. The G521A mutant was 50-fold less active than wild-type LigA, and the G521V and G521D proteins had Ͻ0.1% of wild-type function (Fig.  3B). Modeling the mutations into the LigA-DNA crystal structure indicated that the valine and aspartates clashed with the bound DNA in each of the oxyanion holes, as did alanines (to a lesser extent). It is not obvious why Gly 521 was especially affected by the alanine mutation. It is possible that LigA is less able to adapt to the G521A change by adjusting the local main-chain conformation. We surmise from these results that each of the four DNA-binding oxyanion holes of the HhH is important for E. coli LigA function.
We also tested the effects of alanine substitutions at the four polar residues (Asp 452 , Arg 487 , Glu 519 , or Asp 551 ) of the respective loop-helix motifs positioned near or in the minor groove. The D452A, E519A, and D551A mutants (Fig. 3A) were 68, 120, and 71% as active as wild-type LigA, respectively (Fig. 3B), signifying that none of these acidic residues are important for nick sealing. By contrast, changing Arg 487 to alanine reduced activity to 6% of wild type (Fig. 3B). Conservative substitutions with lysine and glutamine (Fig. 4A) each restored activity to one-fourth of the wild-type level (Fig.  4B). We surmise from the mutational effects that Arg 487 makes important hydrogen-bonding interactions. Indeed, the crystal structure shows that Arg 487 penetrates deep into the minor groove and donates multiple hydrogen bonds to the nucleosides of the 5Ј-AAT trinucleotide of the template strand on the 3Ј-OH side of the nick (Figs. 1 and 5C), specifically from Arg 487 NH2 to the thymidine deoxyribose O4Ј and adenine N3 (5Ј-AAT) and from Arg 487 NH1 to the same adenine N3 (5Ј-AAT). Arg 487 makes water-mediated contacts with the adenosine deoxyribose O4Ј (5Ј-AAT) and adenine N3 (5Ј-AAT) (Fig. 5C). Arg 487 also makes van der Waals interactions with deoxynucleoside sugars of both DNA strands lining the minor groove (Fig. 5C). Either lysine or glutamine could sustain the van der Waals contacts and some of the hydrogen-bonding interactions of Arg 487 , thereby accounting for their ability to restore function partially. Arg 487 is either conserved or replaced by lysine in other bacterial LigA proteins (Fig. 5C).
Thr 524 in the 519 EVGEAT 524 loop-helix motif donates a hydrogen bond from O␥ to a nonbridging oxygen of the phosphate in the oxyanion hole. This is the only instance in the crystal structure of a contact of a side chain of the HhH domain with a DNA phosphate group. The main-chain amide of Thr 524 also donates a hydrogen bond to the same nonbridging phosphate oxygen. Replacing Thr 524 with alanine reduced nick sealing activity to 13% of wild type (Fig. 3B). Although this effect did not meet our cut-off criterion, we proceeded to test conservative serine and valine mutations and found that they increased activity to 48 and 38% of wild type, respectively (Fig. 4B).
Probing the Role of Interdomain Contacts of the HhH Domain Implicated in Clamp Closure-Although the formation of the LigA clamp is driven by the numerous DNA contacts discussed above, it also involves osculating interactions between the NTase domain and HhH domain (Fig. 5B). A detailed view of these contacts shows that they occur in the DNA major groove opposite the nick, where Arg 446 and Arg 447 of the HhH domain donate hydrogen bonds to the backbone carbonyls of NTase residues Gly 137 and Asp 138 , and there is a network of watermediated hydrogen bonds between the Asp 138 , Arg 510 , and Asp 450 side chains (Fig. 5B). To address the functional significance of these contacts, the HhH domain residues Asp 450 and Arg 510 were replaced individually by alanine (Fig. 3A). The R510A mutant was fully competent in nick sealing, and the D450A mutant was 55% as active as wild-type LigA. It was reported previously that changing Asp 138 to alanine had only a mild effect on nick sealing activity in vitro (38% of wild type) and did not affect LigA activity in vivo (13). Thus, the watermediated contacts of Asp 540 and Arg 510 to Asp 138 are not functionally important.
The Arg 446 -Arg 447 dipeptide was doubly substituted with alanine (Fig. 3A). This change elicited a 40-fold decrement in ligation (Fig. 3B). We then purified the R446A and R447A single mutants (Fig. 4A); their specific activities were 5.4 and 21% of wild-type LigA, respectively (Fig. 4B). We surmise that Arg 446 is critical per se for optimal nick sealing activity via its promotion of clamp closure. Although the mutational effects at Arg 447 did not meet our criterion of significance, the 5-fold activity decrement of R447A suggests that its contacts to the NTase domain provide some added clamp stability. Structure-activity relations at Arg 446 were obtained by testing conservative substitutions with lysine and glutamine (Fig. 4A). Lysine restored activity to 36% of wild type, whereas glutamine afforded 14% of wild-type function. These results suggest that hydrogen bonding is the relevant property of Arg 446 , consistent with its contacts to the main chain of the NTase domain. Note that neither Arg 446 nor Arg 447 contacts the DNA in the crystal structure.
Incremental Deletions of the BRCT Domain-Electron density for the BRCT domain was not visible in the crystals of DNA-bound E. coli LigA (8). The BRCT domain was also not visualized in the crystal structure of T. filiformis LigA (see corrected Protein Data Bank code 1V9P). A solution NMR structure of the isolated BRCT domain of T. thermophilus LigA is available in the Protein Data Bank. 3 The LigA BRCT fold comprises a four-strand parallel ␤ sheet overlaid by three ␣ helices (Fig. 6). Because the primary structures of the T. thermophilus and E. coli LigA BRCT domains are well conserved (37/79 positions of side chain identity plus 12/79 positions of side chain similarity; Fig. 6A), we regard the NMR structure of the T. thermophilus BRCT domain (Fig. 6B) as a surrogate for the homologous E. coli module and have exploited it to guide a mutational analysis. Initially, we generated three serial C-terminal truncations, LigA-(1-661), LigA-(1-643), and LigA-(1-636), designed to cleanly delete secondary structure elements of the BRCT domain (Fig. 6A). We also constructed a complete deletion of the BRCT domain, LigA-(1-586), that reprises the form of E. coli LigA that was visible in the DNA-bound crystal structure (8). The wild-type and truncated proteins were produced as His-tagged derivatives and displayed the expected decrements in size when analyzed by SDS-PAGE (not shown). Complete deletion of the BRCT domain reduced specific activity in nick sealing by LigA-(1-586) to 0.14% of the wild-type value (not shown). The specific activities of the LigA-(1-661), LigA-(1-643), and LigA-(1-636) proteins were Յ0.1% of the wildtype LigA activity (not shown). Thus, deletion of all or part of the BRCT domain elicits the same three order of magnitude decrement in nick sealing.
Our findings concerning the essentiality of the BRCT domain for E. coli LigA activity agree with previous conclusions concerning the critical role of the BRCT domain in LigA enzymes from Mycobacterium tuberculosis, Thermus species AK16D, and mimivirus (11,22,23). However, our results disagree with the report of Wilkinson et al. (19) that deletion of the BRCT domain of E. coli LigA resulted in only a 3-fold decrement in the rate of nick sealing by the LigA-(1-592) protein, when compared with their wild-type LigA enzyme. Their LigA enzymes, like ours here, were produced as N-terminal His 10 fusions. The nicked DNA substrates used in the two studies are similar, except that their substrate contained a fluorescein tag at the 5Ј end of the 3Ј-OH strand. To our inspection, the key difference between the two studies is that their wild-type LigA nick sealing rate (0.1 min Ϫ1 ), measured at nearly equimolar concentrations of enzyme (3.5 M) and nicked DNA (5 M), was about 13-fold less than our LigA activity (26 fmol ligation/fmol enzyme/20 min), calculated from the slope of a LigA titration curve under conditions of DNA excess at much lower DNA concentrations (50 nM). We have measured the rate of nick sealing by our LigA preparation at equimolar concentrations of enzyme and nicked DNA (50 nM); the reaction attained 67% of the end point value in 10 s (shorter reaction times were not tested). The calculated turnover number of 3.4 min Ϫ1 under these conditions was some 30-fold higher than that observed by Wilkinson et al. (19). Given the widely posited role for the LigA BRCT domain in DNA binding (11, 16,17,19,22,23), we surmise that the use of 100-fold higher concentrations of DNA and ⌬BRCT protein by Wilkinson et al. (19) might have mitigated the impact of the domain deletion in the sealing assay. This factor, and the comparatively feeble activity of their wild-type LigA preparation, could account for the disparity between their results and ours concerning the impact of BRCT deletions.
Effects of BRCT Deletions on AMPdependent Supercoil Relaxation Suggest a Role in DNA Binding-The structure of DNA-bound E. coli LigA (8) shows that the nick is covered completely by the NTase and OB domains (Fig. 1). Thus, the widely invoked role of the BRCT domain in DNA binding by LigA cannot be via direct nick recognition. Rather, the BRCT domain might enhance DNA affinity by: (i) promoting closure of the protein clamp around the duplex, via interdomain interactions, and/or (ii) contacting the DNA flanking the nick. To gauge the contributions of the BRCT domain to duplex DNA binding, we turned to an assay of LigA-catalyzed relaxation of DNA supercoils (25). This assay takes advantage of the microscopic reversibility of step 3 of the ligation reaction, whereby ligase apoenzyme can, in the presence of high concentrations of AMP and a divalent cation, catalyze attack by an AMP phosphate oxygen on the DNA phosphodiester backbone to form a nicked DNA-adenylate that is then resealed by forward catalysis of step 3. If the starting DNA substrate is a covalently closed supercoiled circular plasmid DNA, and if ligase releases the 3Ј-OH end of the AppDNA nick before executing forward step 3, the net result is incremental supercoil relaxation.
Full-length LigA relaxed negatively supercoiled plasmid DNA in the presence of 10 mM AMP to generate a mixture of partially relaxed topoisomers and fully relaxed circles plus a minor fraction of nicked circular products (Fig. 7). No supercoil relaxation by LigA was detected when AMP was omitted (data FIGURE 6. Mutational analysis of the BRCT domain of LigA. A, the amino acid sequence of the E. coli LigA BRCT domain (Eco) is aligned to the homologous segments of LigA enzymes from M. tuberculosis (Mtu) and T. thermophilus (Tth). The secondary structure elements of the TthLigA BRCT domain are displayed above the sequence, with ␤ strands depicted as arrows and ␣ helices depicted as cylinders. Positions of side-chain identity/similarity between TthLigA and EcoLigA BRCT domains are indicated by dots below the sequence. The C termini of serially deleted versions of EcoLigA studied herein are indicated below the sequence. Conserved basic amino acids that were subjected to alanine scanning are highlighted in green boxes. B, stereo view of the NMR structure of the TthLigA BRCT domain (Protein Data Bank code 1L7B). The side chains equivalent to those chosen for mutational analysis in Eco-LigA are shown as sticks and labeled with the EcoLigA residue numbers. C, aliquots (8 g) of the nickel-agarose preparations of wild-type (WT) LigA and the indicated mutants were analyzed by SDS-PAGE. The Coomassie Bluestained gel is shown. The positions and sizes (kDa) of marker polypeptides are indicated on the left. D, the specific activities of the mutant LigA proteins in nick sealing were determined by enzyme titration and normalized to that of wild-type LigA purified and assayed in parallel with the mutants. (Fig. 8). The BRCT domain mutations R614A and K627A strongly suppressed supercoil relaxation; K648A slowed relaxation by about an order of magnitude and K651A had little effect (Fig. 8).
Conclusions-By using the crystal structure of the LigA⅐ DNA-adenylate complex to guide mutational analyses of the OB and HhH domains, we defined functionally relevant components of the DNA-binding surface and amino acids that aid in forming the C-shaped protein clamp around the DNA helix. Studies of the LigA BRCT domain highlight its necessity for nick sealing.