HIV–1 Antigens in Neurons of Cocaine-Abusing Patients

Cocaine opens the blood-brain barrier by deregulating transcription of target genes. Here we show that cocaine at blood concentrations in drug abusers disrupts endothelial cell junctions in parallel with signaling by phosphorylation of extracellular signal-regulated kinase, myristoylated alanine-rich C kinase and myosin light chain. Cocaine effects may be important in vivo since the neurons of drug abusing patients with HIV-1 associated dementia displayed gp120, p24 and Nef.


INTRODUCTION
Although the incidence of HIV-1-associated dementia (HAD) has been decreasing since the advent of HAART, the prevalence of chronic neurological disease associated with HIV-1 has been increasing. Drug abuse may be an important risk factor in chronic neurological complications of HIV-1 infection. There are compelling reasons why cocaine abuse might increase the risk of HIV-1 infection of the brain [1]. Cocaine opens the blood-brain barrier to HIV-1 [2] by remodeling brain microvascular endothelial cells (BMVEC's) and disrupting virus macropinocytosis and degradation in lysosomes [3].
We have shown that HIV-1 may use two different mechanisms to penetrate the blood-brain barrier (BBB): a "Trojanhorse" transport into the brain in monocytes and lymphocytes [4,5] or macropinocytosis of cell-free virus in BMVEC's [6]. In this study, we examined the concentrations of cocaine patients had a history of drug abuse including cocaine with additional alcohol and opiate abuse in some; the last patient had no history of drug abuse) and two amyotrophic lateral sclerosis (ALS) brain tissues. The autopsy interval ranged between 5 to 6 h except that of one ALS patient, which was 22 h. The Manhattan HIV Brain Bank provided brain tissue of a patient with minor cognitive impairment who had brain lymphoma and was taking medically prescribed opiates. The UCLA Brain Bank provided HIV-1-negative control tissues (amyotrophic lateral sclerosis) and two brain tissues of HAD patients who died in the late 1980's. The protocol was reviewed and approved by the UCLA Institutional Review Board.

Immunohistochemistry (IHC)
Tissues were subjected to antigen retrieval solution (DAKO) in a steamer for 20 min and were treated with Dual endogenous enzyme block and Protein block (DAKO, Carpinteria, California, USA). IHC was performed by the Envision technique (with primary rabbit and mouse antibodies) or the LSAB 2 technique (with primary goat antibodies) (DAKO) as previously described [7]. The primary antibodies were anti-gp120, anti-p24, anti-Nef (all three, which are described below, were provided by the AIDS Research and Reference Reagent Program, NIH, Bethesda, USA), anti-NeuN (Chemicon, Temecula, California, USA), anti-CD68 and anti-GFAP (DAKO).
Two gp120 monoclonal antibodies were used: ID6 [8] and 670-30D [9]. The murine ID6 monoclonal antibody was generated after immunization of a mouse with a recombinant gp160 immunogen. Subsequent analysis using screening of phage display libraries with ID6 indicated that the epitope was contained within amino acids 86-100 of the highly conserved C1 region of gp120. One of the phage mimotopes generated by screening with ID6 (designated phage 3.3) bound to ID6 with a high affinity. Two IHC studies were done using ID6 absorbed with the relevant gp120 epitope. In the first, equal volumes of the ID6 antibody (1:100) and recombinant gp120 JR-FL (250 ng/μL) were incubated at 37 o C for 3 hrs and, after ultracentrifugation, the top half of the suspension was used in IHC. Recombinant gp120 was produced in Chinese hamster ovary cells as described [10]. In the second study, ID6 antibody (1:100) was incubated with phage 2.13 (negative phage mimotope) or phage 3.3 (positive phage mimotope) [8] (each at 1:800) and processed as above.

Transmission Electron Microscopy (TEM)
BMVEC cultures grown on cover slips in 24-well plastic dishes were fixed by immersion according to standard protocols that take into consideration our desire to produce excellent ultrastructural preservation of EC cytoskeletal filaments, microtubules, and EC junctional complexes. For this reason, we fixed the BMVEC at room temperature (25EC) to avoid disruption of EC cytoskeletal components such as microtubules. The fixative contained 2.5-3.0% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4 [11]. BMVEC were post-fixed with 1% osmium tetroxide, dehydrated in a graded series of ethanol, and en bloc stained with uranyl acetate [12]. The coverslips were infiltrated with liquid epoxy plastic overnight in a desiccator at 25EC. On the next day, in liquid plastic, the coverslips were sliced into several pieces and flat-embedded with the BMVEC positioned upward then polymerized in a 60EC oven overnight. For TEM and HVEM studies, plastic thick-and thin-sections were cut with either the Leica Ultracut UCT and Sorvall (MT-1) Ultramicrotomes and stained with lead and uranyl salts, according to standard protocols (Hayat, 1981). Thin-sections were collected on grids, stained with uranium and lead salts and examined with the FEI Morgagni Electron Microscope.

Western Blot Analysis
Confluent monolayers of BMVEC's were incubated overnight in serum-free DME/F12 medium and treated with cocaine as indicated. Cell extracts were prepared by washing cell monolayers three times with phosphate buffered saline (PBS), scraping the cells into SDS sample buffer (125 mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 2% 2mercaptoethanol), then passing them through a 26-gauge needle. After 5 min of boiling, the protein samples were centrifuged for 2 min and separated by sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-PAGE) [13]; the proteins were transferred to nitrocellulose membranes (30 V for 18 h or 90 V for 2 h) [14] and incubated with specific antibodies. The antibodies of interest included anti-phosphomyosin light chain 2 and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cell Signaling, Danvers, Massachusetts, USA); anti-phospho-extracellular signal-regulated kinases 1 and 2 (Erk1/2) (New England BioLab, Beverly, Massachusetts, USA); anti-phosphor-specific myristoylated alanine-rich C kinase substrate (MARCKS) (ProteinTech Group, Chicago, Illinois, USA); and anti-myosin light chain (MLC) (Sigma, St. Louis, Missouri, USA). Immunoreactive proteins were detected with enhanced chemiluminescent detection system (ECL) according to the manufacturer's directions (Amersham, Little Chalfont, UK). The experiments were repeated three times. To evaluate the protein loading, the membranes were stained with GAPDH or MLC antibody.
Cocaine concentrations 10 -8 M and below had minimal effects on BMVEC's. Benztropine is a therapeutic agent that, in addition to binding to the biogenic amine transporters and muscarinic receptors, binds with high affinity to a cocainebinding site on BMVEC's [3]. Similarly as cocaine, 10 -5 to 10 -7 M benztropine produced gaps between endothelial cells (not shown). 10 -5 to 10 -7 M cocaine treatment of BMVEC's induced phosphorylation of Erk 1/2, MARSKC and MLC (Fig. 2). Cocaine concentrations10 -8 and below did not consistently induce cell signaling. Benztropine has a higher affinity for the cocaine-binding site on BMVEC's than cocaine [3]. Accordingly, stimulation by 10 -6 M benztropine induced more prolonged signaling through Erk1/2 and MLC phosphorylation in comparison to cocaine (Fig. 2).

Ultrastructural Features of Monocyte Migration Across BMVEC's
In the BBB model, monocyte migration was stimulated by MCP-1 in the lower chamber. Monocytes migrated between the endothelial cells, which became thick above each pore (Fig. 3A). Monocytes migrated through the pore into the brain chamber (Fig. 3B). The rate of monocyte migration was increased by cocaine, as previously published [2]. 24-h treatment of BMVEC's with 10 -6 M cocaine produced robust vacuolization of the endothelial cell cytoplasm (Fig. 4) and increased monocyte transmigration, as previously published [15].  Fig. (4). Ultrastructural features of monocyte migration through the BMVEC model of the blood-brain barrier. Compared to the untreated cells (a), cocaine-treated BMVEC's (b) display cytoplasmic vacuolization (TEM, 10,000 x).
As shown above (Fig. 1), cocaine at the concentrations in the plasma of cocaine's addicts (10 -6 M to 10 -8 M) disrupts BBB. We have searched for increased evidence of HIV-1 in brain tissues of drug-abusing patients with HAD.

Frontal Lobe Neurons of Drug-Using Patients are Positive for gp120, p24 and Nef
To detect viral "foot-prints" in brain tissues, we performed IHC with the monoclonal gp120 antibody ID6 with brain tissues of six HAD patients, four with a history of drug abuse, one with a history of opiate use for medical reasons, and one without any history of drug use. All patients were intensively treated until their death with HAART. The neuropathology was remarkable for absence of perivascular infiltration by macrophages and lack of gp120 staining of microglia, which were noted in brain tissues of patients with HAD who died in the eighties (Fig. 5). The brain tissues of the patient without a history of drug abuse were completely negative for gp120 ( Table 1).
However, the five patients with a history of drug abuse or medical use of morphine had expression of gp120 (brown) in neurons, as shown by double staining with MAP-2 antibody (red) (Fig. 6a), but not in astrocytes (Fig. 6b).
To confirm the specificity of gp120 staining with the ID6 antibody, this antibody was absorbed with the phage 3.3 mimotope (Fig. 6d), which removed the reactivity, whereas absorption with the unrelated phage 2.13 had no effect on the staining (Fig. 6c). Absorption of the ID6 antibody with gp120 also reduced neuronal staining (not shown).
Furthermore, ID6 antibody did not stain neurons of control patients (e.g., ALS case) and unrelated antibody (CD20) did not stain neurons in HAD brain tissues. In addition, neurons in the frontal lobe tissues were positively stained by two different p24 (Fig. 7a,b) and two different Nef antibodies (Fig. 7c,d).

DISCUSSION
We previously demonstrated that cocaine "opens" the BBB constructed with BMVEC's to HIV-1 entry [2]. In addition, cocaine induced transcriptional alterations in BMVEC's genes important for cell signaling, cytoskeleton organization, cell swelling, vesicular trafficking, and cell adhesion, which may lead to acute and long-term changes in the properties of the blood-brain barrier. Cocaine induced gaps between endothelial cells as early as 30 min after exposure [16]. However, since these effects involved 10 -5 or 10 -6 M concentrations, some doubt has persisted about the in vivo effects of cocaine abuse associated with plasma concentrations below 10 -6 M. Here we show that cocaine concentrations as low as 10 -7 M induce cell signaling in parallel with disruption of cell junctions. Cocaine activates mitogen activated protein kinase (MAPK), protein kinase C (PKC), and MLC kinase pathways in BMVEC's as shown by an increase in ERK, MARCKS, and MLC phosphorylation, respectively. Paradoxically, the ERK response to cocaine appeared stronger at 10 -7 M compared to 10 -6 M cocaine. It has been known that cocaine dose response is not always linear. For example Carmaci et al. showed that cocaine might manipulate the cAMP PKA-Sig-1R and the cAMP-ERK-Sig-1R pathways in biphasic fashion [17]. MAPK and PKC path-ways are critically involved in an increase of endothelial paracellular permeability in different types of endothelium [18][19][20]. In addition, cocaine increased MLC phosphorylation, which initiates EC contraction and leads to gap formation and permeability increase [18,21]. The effects of cocaine appear to be pharmacological, since they are induced through a common binding site with benztropine [3].
HIV-1 neurotoxicity is attributed by one school to indirect mechanisms and by another school to direct virus presence in neurons. The indirect mechanisms, which have been more extensively investigated [22], are mediated by macrophage products, such as tumor necrosis factor-, plateletactivating factor, arachidonic acid and its metabolites, nitric oxide, quinolinic acid, and glutamate [23][24][25], and HIV-1 proteins, such as Tat and gp120, produced by macrophages and microglia [26][27][28]. Human studies of peripheral blood of HAD patients noted elevated cytokines and neurotoxins [22], and macrophage-specific proteins [29]. However, although some investigators still believe that neuronal toxicity is due solely to indirect mechanisms [30,31], neuronal expression of HIV-1 proteins, such as Vpr [32], and neuronal presence of HIV-1 DNA [33] have been described prior to our study and are made plausible by this study. We have shown (a) absence of robust perivascular macrophage infiltration, and (b) presence of HIV-1 antigens, gp120, Nef and p24, in neurons of drug using HAD patients. Our patients received intensive HAART therapy, which curtailed high-grade HIV-1 viremia suffered by patients in 1980's and could have blocked immunological activation and brain migration of macrophages [4]. Interestingly, most patients with HAD seen at UCLA had a history of cocaine abuse, which could be due to the in vivo effects of cocaine showed in vitro by this study. The novel immunohistochemical observations of HIV-1 antigens in neurons of drug abusing HAD patients may be related to the robust effects of co-caine even at the plasma levels below 10 -6 M, which may cause subtle breaks of BBB permitting penetration of viral proteins or infectious virus into the neuropil and, for unknown reason, selective invasion of neurons. In this regards, it is interesting that in a rat model gp120 is transported into cortical neurons by retrograde axonal transport from the striatum and hippocampus [34].

ACKNOWLEDGEMENTS
This project was supported by Grants HL63639 and HL63065 from the National Institutes of Health (to M.F.)