Spermidine Feeding Decreases Age-Related Locomotor Activity Loss and Induces Changes in Lipid Composition

Spermidine is a natural polyamine involved in many important cellular functions, whose supplementation in food or water increases life span and stress resistance in several model organisms. In this work, we expand spermidine’s range of age-related beneficial effects by demonstrating that it is also able to improve locomotor performance in aged flies. Spermidine’s mechanism of action on aging has been primarily related to general protein hypoacetylation that subsequently induces autophagy. Here, we suggest that the molecular targets of spermidine also include lipid metabolism: Spermidine-fed flies contain more triglycerides and show altered fatty acid and phospholipid profiles. We further determine that most of these metabolic changes are regulated through autophagy. Collectively, our data suggests an additional and novel lipid-mediated mechanism of action for spermidine-induced autophagy.


Introduction
The understanding of the mechanisms underlying the aging process is of general interest; not least because it opens doors to modulate it and eventually postpone or prevent age-related pathologies and thus improve our health span. Aging research in the last three decades has elucidated a series of mutations in single genes that increase life span in many organisms [1]. In addition, dietary restriction (DR) has advanced to be the most reliable means to increase life span and reduce age-related diseases in many organisms [2], although the latest results from studies in non-human primates are not as promising as expected, at least in regard to life span [3]. Despite these findings, the most convenient method to counteract the detrimental effects of aging would be the simple ingestion of compounds with the ability to do so. In fact, several pharmacological interventions have been shown to hamper age-related diseases and to be beneficial for health and life span. For instance, resveratrol, a naturally occurring phenol, increases the life span of mice kept on a high-fat diet [4]. However, resveratrol does not exert any beneficial effect in healthy organisms [5]. The immunosuppressant drug rapamycin also increases the life span of rodents [5,6], but shows inconclusive effects in the fruit fly Drosophila melanogaster and -being an immunosuppressant -its actual use for humans remains doubtful [7,8].
Spermidine is a natural polyamine involved in many important molecular processes such as DNA stability, transcription, translation, apoptosis, cell proliferation, differentiation, and survival [9]. Intriguingly, its intracellular level decreases with age [10,11]. Indeed, a recent study shows that spermidine levels decrease in  year-old people compared to  year-olds [12]. However, in that same study levels in 90-106 year-old people were similar to those found in the youngest age group [12], suggesting that maintenance of high spermidine concentration during aging may contribute to longevity. In accordance with this concept, our previous work [13] established that food supplementation with spermidine increases the life span of the yeast Saccharomyces cerevisiae, the nematode worm Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and that of cultured human immune cells. A high-polyamine diet has also been reported to increase the life span and reduce the age-related pathology of a short-lived mouse strain [14]. Spermidine also increases stress resistance in yeast (heat and hydrogen peroxide) [13] and flies (paraquat and hydrogen peroxide) [15], while it decreases markers of age-related oxidative damage in mice [13]. Conversely, the intracellular depletion of spermidine by genetic mutations in the polyamine pathway decreases the life span of yeast [13] and mice [16].
In yeast, worms, flies and Hela cells, spermidine directly induces autophagy [13,17], the major cellular recycling mechanism, which is induced upon many interventions that increase life span [18,19]. In fact, we could previously show that spermidine's beneficial effects on aging are mainly due to the induction of autophagy [13]. Consistently, spermidine does not increase life span in autophagymutant yeast, worms and flies [13]. However, we found more recently that autophagy induction is not responsible for all the beneficial effects exerted by spermidine: For instance, the antinecrotic function of the propeptide of yeast cathepsin D is spermidine-dependent but autophagy-independent [20]. Also, spermidine can still increase resistance to hydrogen peroxide in autophagy-deficient flies [15]. Thus, under certain circumstances additional mechanisms other than autophagy must be responsible for spermidine-mediated cytoprotection.
Metabolic regulation has long been connected to life span modulation: many mutations known to increase life span also alter metabolism and nutrient sensing, for instance mutations in the IIS (Insulin-Like Signalling) pathway [21], the TOR (target of rapamycin) pathway [22], or in sensory systems [23]. Mounting evidence also places lipid metabolism as an important player during aging [24][25][26]. Many interventions that enhance life span, for example, also increase the abundance of triglycerides (TAG), which represents the major lipid store [27,28]. In fact, the metabolic targets associated to spermidine's beneficial effects might be multiple. Its molecular structure allows a plethora of different interactions that might go even beyond multiple ones already described [9,29].
In this work, we expand spermidine's scope of potential beneficial effects during aging by showing that it improves agedependent locomotor activity in D. melanogaster. Importantly, we additionally uncover an impact of spermidine on lipid composition, which involves an increase in weight and TAG levels as well as alterations in fatty acid and lipid profiles, most of which seem to be autophagy-dependent. These results are the first showing spermidine's potential to modulate lipid metabolism and suggest a possible involvement of this capability in the substance's mechanisms to exert beneficial effects during aging.

Spermidine improves locomotor performance in aged flies
We have previously demonstrated that spermidine-fed flies show an improved survival during aging [13]. We therefore decided to first assess if such improvement might correlate with a decreased incidence of an age-related phenomenon, the progressive decline of locomotor activity. For this purpose, we followed the ability of flies to climb the vertical wall of the vial, in which they were kept. The measurement was done weekly until none of the flies could reach the threshold (8 cm in 10 s). Expectedly, the climbing activity of wild type cells diminished with ongoing age (Fig. 1A) [30,31]. Spermidine feeding (1 mM) could, indeed, partly rescue this age-dependent decline in both female and male wild type animals (Fig. 1A, B). Importantly, spermidine did not have such effect in loss-of-function mutants for atg7 (atg7 2/2 ), a gene essential for autophagy. These animals showed a much faster decline in activity compared with wild type flies and no improvement with spermidine ( Fig. 1A, B). Thus, spermidine alleviates age-dependent locomotor impairment in flies in an autophagy-dependent manner.

Spermidine increases stored lipid content and alters lipid profiles
Age-related decline in motor performance is one of the phenotypes arising from neuronal aging, for which recent evidence suggests an important contribution of changes in the lipid membrane composition [32]. In fact, lipid metabolism seems to be a prominent player during aging [24][25][26] and many life spanenhancing interventions also increase the levels of TAG [27,28]. We thus decided to analyze the impact of spermidine feeding on fly lipid profiles. Even though treatment of wild type flies with 1 mM spermidine for one week did not affect food intake, glycogen or protein levels ( Fig. 2A-C), it increased the animals' TAG-content as measured both by thin layer chromatography (Fig. 3A) and a colorimetric assay (Fig. 3B). Interestingly, total and TAG-derived fatty acids displayed specific changes in male and female flies, respectively (Fig. S1, S2, Table S1). Of note, the ratio of saturated over unsaturated TAG-derived fatty acids declined upon spermidine treatment (Fig. 3C). Moreover, lipidomic electrospray-mass spectrometry analysis revealed a significant impact of spermidine on the levels of a multitude of lipid species. All lipid species were fully characterized (Table S2). While the phospholipids profile in the negative ion mode survey scans did not show any significant differences between spermidine-treated and untreated animals in both sexes (Fig. S3, S4), the positive survey revealed changes upon spermidine feeding: males displayed increased levels of EPCd38:1, PC32:2 and PE38 as well as enhanced saturation of EPCd34 (Fig. 4). In females, spermidine induced an increase in PE34:2 and PC34:4 (Fig. 5). Collectively, these findings show that spermidine affects lipid metabolism by virtue of increased TAG levels, altered fatty acids and phospholipids composition.

Autophagy regulates spermidine-mediated lipidomic changes
Lipid metabolism has recently been described to act at the crossroads of longevity and autophagy [33]. In order to test whether autophagy is connected to spermidine-mediated lipid changes, we analyzed this aspect in autophagy-deficient mutants (atg7 2/2 ). It should be noted that these mutant flies showed no significant differences on overall lipid levels ( Fig. 3A, B), even though they did present some changes in their fatty acid profiles (Table S1). Interestingly, spermidine maintained and even exacerbated its enhancing effect on TAG levels ( Fig. 3A, B) as well as on sex-specific changes in total and TAG-derived fatty acids (Fig. S1, S2, Table S1), with female flies showing a higher and male flies a lower overlapping changing TAG-derived fatty acids compared with those observed in wild type flies (Table S1). The decline observed in the ratio of saturated over unsaturated TAG-derived fatty acids was also maintained (Fig. 3C). Thus, the general TAG-increase observed with spermidine is autophagyindependent, although the level of TAG itself may be autophagydependent. In contrast, the profile of TAG-derived fatty acids triggered by spermidine seems to depend on functional autophagy. Intriguingly, spermidine failed to induce the effects on phospholipid profiles observed in wild type animals: In atg7 2/2 females, no differences whatsoever were observed ( Fig. 5) while in atg7 2/2 males spermidine decreased the saturation of EPCd34 as opposed to wild type animals ( Fig. 4). Altogether, this shows that spermidine exerts its effects on TAG-derived fatty acid and lipid profiles in an autophagy-dependent fashion.

Discussion
Our previous work has demonstrated the beneficial potential of spermidine in terms of age-related stress resistance and survival during aging [13,15]. In this study, we expand these findings by reporting a correlation of these benefits with a decreased incidence of the age-related decline in locomotor activity. Interestingly, a putative effect of polyamines on enhanced activity during aging has been previously anecdotally reported for mice fed a highpolyamine diet, which were described to be more active and to keep a thicker coat with age, but these variables were not measured [14]. Furthermore, we had already reported that spermidine rescues the decline in activity of flies under paraquat exposure [15]. In contrast to our results, a recent report showed no protective effects of spermidine on the age-dependent decline of climbing activity in flies [34]. While both studies use a similar protocol to assess climbing activity, we do find a positive effect in both sexes. Two main reasons may account for this discrepancy. Firstly, Gupta et al. used a much smaller sample size: one group of ten flies [34] compared with five groups of ten flies in the present study. Secondly, the percentage of old flies (30 days of age in that study) reaching the threshold is much lower than the percentage we report here at similar ages (between 4 and 5 weeks): around 45% in that report [34] versus approximately 80% in our study. Given the higher sample size and the seemingly more optimal physiological state of the flies used in this study, we rely on the herein reported data in anticipation of further studies that may resolve these varying results.
Age-dependent decline in motor performance has been suggested to be linked to lipid metabolism [32], which in general seems to be connected to the aging process [24][25][26]35,36]. Intriguingly, spermidine has been recently identified and characterized as a key factor in the differentiation of preadipocytes into mature adipocytes, and thus in the process of adipogenesis [37,38].
However, our results cannot answer whether changes in lipid level and composition are the causes for spermidine-mediated locomotor improvement. Locomotion is controlled by two main organs/ tissues: brain/neurons and muscles. On the one hand, it is known that spermidine interacts with brain cells: It has anti-inflammatory effects on microglia cells subjected to lipopolyssacharides exposure [39] and spermidine levels are linked to memory [34] as well as to disorders like Alzheimer's disease [40]. It would thus be no surprise that spermidine improves age-related locomotion by altering neuronal cells or communication between neurons and muscles. On the other hand, the effects of polyamines on muscle function have been much less studied and have focused mainly on smooth muscles, especially heart muscle cells. Anabolic agents have been shown to increase both skeletal muscle mass and  . Food intake, glycogen and protein contents. A) Mean +/2 SEM of food intake in wild type flies of both sexes fed 1 mM spermidine or not as measured by colorimetry (flies fed blue dye and dye intensity measured). 2 independent replicates were monitored (5 or 6 independent samples for each group in each replicate). B, C) Mean +/2 SEM of glycogen (B) and protein (C) content in wild type or atg7 2/2 flies of both sexes fed 1 mM spermidine or not as measured by colorimetry (anthrone reaction for B and Bradford reaction for C). 3 independent replicates were monitored (4 or 5 independent samples for each group in each replicate). **p,0.01; ***p,0.001. doi:10.1371/journal.pone.0102435.g002 polyamines levels, but a causal link between polyamines and muscle growth has not been investigated [41]. Of note, the initiation factor eIF5A, whose synthesis requires spermidine, increases skeletal muscle stem cell differentiation [42]. Since skeletal muscle is one of the tissues most prone to aging (sarcopenia, [43]), it is also equally plausible that spermidine acts directly or indirectly on muscles to improve locomotion in old age.
Our herein presented results suggest that at least some spermidine-mediated beneficial effects might be, indeed, related to lipid changes. For instance, we report that spermidine-fed flies show increased TAG levels, which has often been associated with increased life span [27,28,44,45]. In a similar manner, long-lived Drosophila (upon overexpression of dSir2) show an up-regulation in the expression of genes involved in fat metabolism [46] and, in turn, increased expression of genes related to fatty acid b-oxidation extends life span in flies [47]. In mice, the ability to maintain fat content under dietary restriction (DR) correlates with life span extension upon DR [26]. In light of our findings, we reanalysed our previously published microarray data on the effect of spermidine on gene expression in yeast, focusing on genes related to lipid metabolism (data available at https://www.ebi.ac.uk/ arrayexpress/experiments/E-MTAB-912/ [48], previously published in 13). In support of the present results, spermidine alters the expression of many genes involved in lipid metabolism, some being up-regulated (e,g., FAA1 and FAA4, long-chain fatty acyl-CoA synthetases or SKN1, involved in sphingolipid biosynthesis) and some being down-regulated (e.g., PIS1, a phosphatidylinositol synthase or ISC1, inositol phosphosphingolipid phospholipase C), showing that spermidine profoundly regulates the expression of lipid-associated genes.
Whether spermidine-mediated TAG increase can be associated to spermidine-induced life span extension is not clear, since autophagy mutants, in which spermidine fails to trigger longevity [13], display an even higher TAG-content. Thus, the observed TAG increase could be the result of spermidine's autophagyindependent survival effects [15,20], or other molecular or organismal consequences of spermidine treatment. Among the diverse lipid species that play a role in metabolism, fatty acids (FFAs), diacylglycerol (DAG), ceramide and cholesterol are considered the ones bearing lipotoxic potential, whereas TAG is generally thought as being a rather inert fat store. Of course, massive TAG deposition in various tissues other than adipose tissue (e.g. muscle, heart, liver) is considered lipotoxic [49], but TAG primarily serves at detoxifying FFAs and DAG than being toxic itself. Consequently, FFAs and DAG steady state levels are very low and they only serve as metabolic intermediates. An increase in TAG as a response to spermidine feeding could thus mean that this detoxification capacity is enhanced. Possibly, autophagy is directly involved in the process of lipid remodeling. A connection of autophagy to lipid turnover has been previously identified [50], but the extent of its interconnection might still be underestimated. Thus, the actual relevance of the herein described TAG-increase upon spermidine supplementation, i.e., an optimal amount for improved fitness, will have to be clarified in future studies.
Besides a higher TAG content, spermidine also altered TAGderived fatty acids towards a decreased ratio of saturation to unsaturation. Moreover, spermidine triggered profound changes in phospholipid profiles. Among these alterations, the increased saturation of ceramide phosphoethanolamine (CPE -or ethanolamine phosphate-ceramide species) EPCd34 in male flies appears most interesting. CPE is the main sphingolipid in invertebrates [51] and involved in signalling as well as an essential membrane component that provides scaffolding for organisation and stability. In this light, the effects of spermidine on EPCd34 saturation in males may affect signalling and membrane fluidity, which would have important consequences for aging. For instance, mitochondrial membrane fluidity is a critical factor during aging and neurodegeneration [52] and may modulate the response to high oxidative stress deriving from molecular damage. Further experiments will have to address whether there exists a causal relationship between changes in membrane fluidity and the beneficial effects of spermidine. It must be noted that we could not measure any changes in EPCd34 saturation of female flies. However, this particular lipid species is much less abundant in females and here a more fine-tuned modulation might occur that could not be detected in this study. We found that spermidine has qualitatively or quantitatively different effects on lipid levels and composition in males and females. Indeed, male and female flies exhibit different lipid profiles, especially differences in saturation levels in various lipid species [53]. Given the different nature of lipids, it is not surprising that spermidine may affect them differently. This may partly explain the differences of sexes we have observed on other variables upon spermidine treatment, e.g., life span [13], resistance to stress [15] or locomotor activity (this study).
According to our results, the above delineated qualitative and quantitative impact of spermidine on lipid levels and composition partly seems to depend on autophagy. Autophagy deficiency eliminates most of the effects of spermidine on lipid profiles or even reverses them as with the saturation ratio of EPCd34. On the other hand, lack of autophagy exacerbates spermidine-mediated TAG-accumulation, which as discussed above rather suggests that this effect is not associated to spermidine-mediated longevity, which is mainly autophagy-dependent [13]. Nevertheless, some of the other lipidomic changes (saturation ratio and fatty acid and phospholipid profiles) observed in spermidine-treated wild type flies and abrogated in autophagy-mutant animals might be linked to spermidine-induced longevity since the modulation of lipid metabolism has been recently connected to autophagy and longevity [25,33]. Still, the general role of autophagy on lipid metabolism remains a matter of debate as studies report contrasting effects [50,[54][55][56] and thus needs further investigation. Besides these considerations, it should be noted that spermidine seems to trigger metabolic effects beyond the lipid realm: for instance, while spermidine-treatment did not lead to any changes in protein or glycogen levels in wild type flies, autophagy-mutant females showed a higher glycogen (Fig. 2B) and males a higher protein level (Fig. 2C). This may point towards further metabolic aspects, in which regulatory processes connecting autophagy and spermidine are involved.
It will be interesting to see whether the herein described modulation of lipid level and composition interacts with some of the previously described mechanisms of spermidine-mediated beneficial effects, including general hypoacetylation and phospho-regulatory pathways [13,57,58] or PKB/Akt [59,60] and the MAPK pathway [60][61][62][63]. In fact, flies knocked-down for Loco, which regulates inhibitory G proteins that in turn regulate MAPK [64,65], show increased life span and enhanced stress that is associated with an increase in TAG levels compared with controls. These phenotypes are similar to those observed in spermidinetreated flies. Intriguingly, Loco knocked-down flies exhibit decreased cAMP levels, which may represent a molecular link connecting spermidine, MAPK and lipid metabolism and thus be worthy of future studies.
In summary, we here uncover the far-reaching impact of spermidine treatment on lipid levels and composition, which at least in part seems to be autophagy-dependent. It is tempting to theorize that these spermidine-induced changes might be involved in the plethora of beneficial effects that spermidine exhibits during stress and aging. Future studies will have to clarify the causality of the herein described phenomenon in order to establish a further branch of action in spermidine's extensive impact on cellular metabolism.

Flies Husbandry
The atg7 2/2 flies were kindly provided by T. Neufeld (University of Minnesota) [66]. Flies were collected as virgins and were kept unless otherwise stated on a standard cornmealsugar-yeast diet at 25uC. Males and females were kept separately. The food was changed twice a week and supplemented or not with spermidine (Sigma, S4139) at a 1 mM final concentration.

Triglycerides content
The protocol for measurement of triglycerides content by thin layer chromatography is as described in Al-Anzi et al. [67]. In each vial, 20 flies were kept and given spermidine or not for one week, after which the flies were then frozen in liquid nitrogen. Ten flies were homogenized in 250 ml of a 2:1 chloroform (VWR, 100777C):methanol (Sigma, 34860) solution, and 2 ml were spotted onto a TLC plate (VWR, 1.16835.0001). Each plate was run in 4:1 hexane (Sigma, 270504):ethyl ether (Fisher Scientific, D/2506/15) until the solvent reached almost the top of the plate. The plate was then dipped in ceric ammonium molybdate (2.5 g ammonium heptamolydbate tetrahydrate (Sigma, 431346), 1 g cerium (IV) sulphate hydrate complex with sulfuric acid (Sigma, 423351), 90 ml water, 10 ml concentrated sulfuric acid (Sigma, 258105). The plates were then dried with a hair dryer and a picture taken with an UVIdoc HD2 system (Uvitec). The area and mean grey value of the triglycerides spots were measured with ImageJ (http://rsbweb.nih.gov/ij/). Lard was run on each plate as a control. Three independent replicates were performed, each with four independent samples for each group. Triglycerides content was also measured using a commercial colorimetric kit (Cambridge Biosciences, Caymen triglyceride colorimetric assay kit 10010303). In each vial, 20 flies were kept and given spermidine or not for one week. Three independent samples were prepared for each group consisting of 10 flies (fresh tissue), which were put in a Eppendorf tube with 200 ml PBS and 750 ml of a 1:2 chloroform:methanol solution. The flies were homogenized and then transferred into glass vials. The samples were then mixed for 15 minutes on a vortex. A further 250 ml of chloroform was added and the samples vortexed, followed by 250 ml of water and vortexed again. The mixture was made biphasic by centrifugation for 5 min at room temperature at 2000 g. The lower phase was transferred into new vials, dried under nitrogen and re-suspended into 600 ml of a 1:2 chloroform:methanol solution. A portion (200 ml) was transferred into a new vial and all vials were dried under nitrogen. The lipids in the vials in which 200 ml were transferred were re-suspended in 250 ml of the standard diluent assay reagent and triglycerides measured following the manufacturer's instructions. Three independent replicates were performed.

Electrospray-mass spectrometry analysis
Lipid extracts from the vials in which 400 ml were transferred, were dissolved in 15 ml of choloroform:methanol (1:2) and 15 ml of acetonitrile:iso-propanol:water (6:7:2) and analyzed with a Absceix 4000 QTrap, a triple quadrupole mass spectrometer equipped with a nanoelectrospray source.
Samples were delivered using either thin-wall nanoflow capillary tips or a Nanomate interface in direct infusion mode (,125 nl/min). The lipid extracts were analyzed in both positive and negative ion modes using a capillary voltage of 1.25 kV. MS/ MS scanning (daughter, precursor and neutral loss scans) were performed using nitrogen as the collision gas with collision energies between 35-90 V. Each spectrum encompasses at least 50 repetitive scans. Tandem  Assignment of phospholipid species is based upon a combination of survey, daughter, precursor and neutral loss scans, as well previous assignments [68]. The identity of phospholipid peaks was verified using the LIPID MAPS: Nature Lipidomics Gateway (www.lipidmaps.org).

Identification and Quantification of Fatty acids
Full characterization and quantification of the fatty acids was conducted by conversion to the corresponding fatty acid methyl esters (FAME) followed by GC-MS analysis. Triplicate lipid extract aliquots were transferred to 2 ml glass vessels and spiked with an internal standard fatty acid C17:0 (20 ml of 1 mM) and dried under nitrogen. Fatty acids were released by base hydrolysis using 500 ml of concentrated ammonia and 50% propan-1-ol (1:1), followed by incubation for 5 h at 50uC.
After cooling the samples were evaporated to dryness with nitrogen and dried twice more from 200 ml of methanol:water (1:1) to remove all traces of ammonia. The protonated fatty acids were now extracted by partitioning between 500 ml of 20 mM HCl and 500 ml of ether, the aqueous phase was re-extracted with fresh ether (500 ml) and the combined ether phases dried under nitrogen in a glass tube. The fatty acids were converted to methyl esters (FAME), by adding diazomethane (3620 ml aliquots) to the dried residue, while on ice. After 30 min the samples were allowed to warm to RT and left to evaporate to dryness in a fume hood. The FAME products were dissolved in 10-20 ml dichloromethane and 1-2 ml analyzed by GC-MS on a Agilent Technologies (GC-6890N, MS detector-5973) with a ZB-5 column (30 M625 mm625 mm, Phenomenex), with a temperature program of at 70uC for 10 min followed by a gradient to 220uC at 5uC/min and held at 220uC for a further 15 min. Mass spectra were acquired from 50-500 amu. The identity of FAMEs was carried out by comparison of the retention time and fragmentation pattern with a FAME standards (Larodan) and bacterial FAME standards (Supelco).

Glycogen and Protein Contents
Glycogen content was measured as described in Van Handel [69]. In each vial, 20 flies were kept and given spermidine or not for one week after which flies were then frozen in liquid nitrogen.
Three flies were homogenized in 100 ml methanol and 50 ml of a saturated sodium sulphate (Sigma, 238597) solution. Samples were then centrifuged at 13000 g for 3 min and the supernatant removed. The pellet was then gently dissolved in 1 ml ultra pure water. Standards (10-50 mg glycogen, Sigma, G0885) were also prepared in 1 ml water. One hundred ml of the samples, standards or blank was then mixed gently with 3 ml of anthrone reagent (dilute 760 ml sulfuric acid into 300 ml water, add 150 mg anthrone (Sigma, 10740) to 100 ml of diluted sulfuric acid just before use), heated at 90uC for 20 min then cooled on ice. A portion of each sample (250 ml), standard and blank was transferred into a 96-well plate and the absorbance read at 630 nm. Three independent replicates were performed, each with 4 or 5 independent samples for each group.
For protein content, in each vial 20 flies were kept and given spermidine or not for one week after which flies were then frozen in liquid nitrogen. Twenty five flies were homogenized in 100 ml PBS and incubate at 70uC for 5 min. The samples were then diluted 1:10 in PBS ad centrifuge at maximum speed for 3 min. Standards (0.5-2 mg/ml) were prepared with albumin (VWR, A16951). Five ml of homogenates, standards or blank were added to 250 ml of Bradford reagent (Sigma, B6916) into a 96-well plate and mix for 30 s. The absorbance was read at 570 nm after leaving the plates 30 min at room temperature. Three independent replicates were performed, each with 4 or 5 independent samples for each group.

Food Intake
Food intake was measured as described in Libert et al. [23] in normal flies. Twenty flies were kept in each vial and given spermidine or not for one week. Then the flies were fed for 24 hrs a 5% sugar/yeast diet mixed with 0.1% of blue dye (food colorant, Dr Oetker). Flies were then frozen in liquid nitrogen. Five females or 8 males were homogenized in 200 ml PBS and centrifuged at 8000 g for 10 minutes. A portion of 150 ml was transferred into a 96-well plate and absorbance was measured at 630 nm. Standards were prepared from aliquots of dyed food to estimate the mass of food consumed. Two independent replicates were performed, each with 5 or 6 independent samples for each group.

Climbing Activity
Flies were kept in individual vials in groups of ten. Once a week, the percentage of flies in each vial able to reach 8 cm in 10 s was measured until no fly could reach the threshold. Three independent replicates were performed. The experiment was performed in both normal and autophagy-deficient flies.

Statistical Analyses
Data from glycogen and protein contents were expressed as content per mg of fly. Triglycerides content by colorimetric assay was expressed in microgram per fly. Data from triglycerides content by thin layer chromatography were weighted according to the weight of the flies in each sample. Data from food intake were expressed as intake per fly. The intensity data from the triglycerides content by thin layer chromatography were transformed into the inverse of the mean grey value to obtain a higher value for darker chromatography spots (the software ImageJ is set up so the darker the spot, the lower the mean grey value). For all Figure 5. Phospholipid profile of females positive survey. A-D) Positive ion ES-MS survey scans (600-1000 m/z) of total lipid extracts from female wild type (A, atg7 +/+ ) and atg7 2/2 (B) flies as well as from spermidine-fed female wild type (C, atg7 +/+ ) and atg7 2/2 (D) flies. Arrows indicate significant changes compared to normal flies untreated. doi:10.1371/journal.pone.0102435.g005 experiments, the effect of spermidine was tested using nonparametric t-tests. Sex and genotype were analysed separately. Figure S1 Total fatty acid profile of males. A-D) Identification and quantification of total fatty acid content from wild-type and atg7 2/2 male flies. Fatty acids from male wild type flies left untreated (A) or fed 1 mM spermidine (C) and male atg7 2/2 mutant flies left untreated (B) or fed 1 mM spermidine (D) were derivatised with diazomethane to the corresponding fatty acid methyl esters (FAMEs), together with an internal standard (C17:0). The samples were analyzed by GC-MS and the retention times and fragmentation patterns, compared with FAME standards. 3 independent replicates were monitored for each group. (TIF) Figure S2 Total fatty acid profile of females. A-D) Identification and quantification of total fatty acid content from wild-type and atg7 2/2 female flies. Fatty acids from female wild type flies left untreated (A) or fed 1 mM spermidine (C) and female atg7 2/2 mutant flies left untreated (B) or fed 1 mM spermidine (D) were derivatised with diazomethane to the corresponding fatty acid methyl esters (FAMEs), together with an internal standard (C17:0). The samples were analysed by GC-MS and the retention times and fragmentation patterns, compared with FAME standards. 3 independent replicates were monitored for each group. (TIF) Figure S3 Phospholipid profile of males negative survey. A-D) Negative ion ES-MS survey scans (600-1000 m/z) of total lipid extracts from male wild type (A, atg7 +/+ ) and atg7 2/2 (B) flies as well as from spermidine-fed male wild type (C, atg7 +/+ ) and atg7 2/2 (D) flies.