Phylogenetic confirmation of the genus Robbea (Nematoda: Desmodoridae, Stilbonematinae) with the description of three new species

ABSTRACT The Stilbonematinae are a monophyletic group of marine nematodes that are characterized by a coat of thiotrophic bacterial symbionts. Among the ten known genera of the Stilbonematinae, the genus Robbea Gerlach 1956 had a problematic taxonomic history of synonymizations and indications of polyphyletic origin. Here we describe three new species of the genus, R. hypermnestra sp. nov., R. ruetzleri sp. nov. and R. agricola sp. nov., using conventional light microscopy, interference contrast microscopy and SEM. We provide 18S rRNA gene sequences of all three species, together with new sequences for the genera Catanema and Leptonemella. Both our morphological analyses as well as our phylogenetic reconstructions corroborate the genus Robbea. In our phylogenetic analysis the three species of the genus Robbea form a distinct clade in the Stilbonematinae radiation and are clearly separated from the clade of the genus Catanema, which has previously been synonymized with Robbea. Surprisingly, in R. hypermnestra sp. nov. all females are intersexes exhibiting male sexual characters. Our extended dataset of Stilbonematinae 18S rRNA genes for the first time allows the identification of the different genera, e.g. in a barcoding approach. http://zoobank.org/urn:lsid:zoobank.org:pub:D37C3F5A-CF2B-40E6-8B09-3C72EEED60B0


Introduction
Stilbonematinae are a marine subfamily within the nematode family Desmodoridae (order Desmodorida) and occur worldwide in sulphidic sediments (reviewed in Tchesunov, 2013). They are characterized by an ectosymbiosis with sulphur-oxidizing bacteria that covers their cuticle in a genus-and sometimes even species-characteristic manner (Polz et al., 1992(Polz et al., , 1994Ott et al., 2004). Although the family Desmodoridae is probably polyphyletic (van Megen et al., 2009), molecular data (Kampfer et al., 1998;van Megen et al., 2009) suggest a monophyly of the subfamily Stilbonematinae. A distinct morphological synapomorphy of the subfamily is the complex glandular sense organ (GSO) described by Nebelsick et al. (1992) and Bauer-Nebelsick et al. (1995), that has so far only been found in members of the Stilbonematinae. This organ produces a special set of lectins (sugar-binding proteins) that seem to be involved in sustaining the specificity of the symbiosis (Bulgheresi et al., 2006. Otherwise very few common features unite the group, such as the lack of a buccal armature and the weak development of the pharynx (Ott et al., 2004). Some characters appear to have developed independently more than once. For example, there is a tendency towards the development of an enlarged muscular portion of the anterior part of the pharynx (corpus), which is conspicuous in all species of the genera Robbea and Catanema, and has been described for the single species of Parabostrichus (Tchesunov et al., 2012), whereas in other genera (Laxus, Leptonemella, Eubostrichus) it is present only in a few species. Other examples are the reduction of the amphidial fovea or the presence of a stiff corpus gelatum protruding from the amphidial fovea (Stilbonema, Catanema, Leptonemella partim) (Tchesunov, 2013). This complicates the assessment of the relationships between the various genera described until now.
In addition, the type species of several genera are either inadequately described or have features which are the exception rather than the rule in the subsequently described members of the genus. Liberal synonymization has added further confusion.
The genus Robbea, introduced by Gerlach (1956), is characterized by the clear separation of a muscular anterior corpus from a mostly glandular isthmus and posterior bulbus which make up the remaining portion of the pharynx. In some species the males have peculiar papillae in the ventromedian line of the cervical and post-cervical region, which resemble suction cups and have a stout conical setae in its centre. These appear to be unique for the genus Robbea and have not been described from other stilbonematine genera.
The aim of this study was to reinvestigate morphological characters that are shared by all species of the genus Robbea but clearly distinguish those from other stilbonematine nematode genera. We describe three new species of Robbea from shallow subtidal sands around the island Carrie Bow Cay on the Belize Barrier Reef (Caribbean Sea). We combine morphological and molecular analyses to clarify the status of the genus and support our findings by providing additional phylogenetic information on previously under-sampled genera.

Fig. 1.
Map showing sampling locations for the nematodes used in this study. 1, Carrie Bow Cay, Belize; 2, Elba, Italy; and 3, Sylt, Germany. Insert shows detailed locations in the vicinity of Carrie Bow Cay where the three new Robbea species were found. Asterisks mark the sampling locations of the individuals that were used for sequencing.
Leptonemella vicina was collected off the island of Sylt, Germany (55 00 0 54 00 N, 08 26 0 16 00 E) with sediment cores from an intertidal sand flat (Fig. 1). Worms were extracted from the sand after anaesthesia with MgCl 2 , shaking and decanting or simply shaking and decanting through a 32 mm mesh sieve. The live animals were sorted using a dissecting microscope and immediately fixed in 4% formaldehyde (for taxonomic preparations) or 2.5% glutaraldehyde in 0.1 sodium cacodylate buffer and postfixed in 2% OsO 4 (for SEM and semi-thin sections). For light microscopy the worms were placed into glycerol: water 1:9, slowly evaporated and finally mounted in pure glycerol; for SEM specimens were critical point-dried and coated with gold. Specimens for sequencing of the 18S rRNA gene were preserved in 70% ethanol or methanol.
Drawings were done using a camera lucida on a Reichert Diavar or a Leitz Ortholux. Nomarsky interference contrast photos were taken on a Reichert Polyvar; SEM on a JEOL JSM-35 CF and a Phillips XL20; semi-thin sections were cut on a Reichert Ultracut and photographed in a Reichert Polyvar.
DNA extraction, PCR amplification and sequencing of the 18S rRNA gene DNA for all sampled species was extracted and purified from single nematode specimens as described before (Schizas et al., 1997). The partial 18S rRNA gene (»1800 bp) was amplified by PCR with the general eukaryotic primers 1F (5 0 -GGTTGATYCTGCCAGT-3 0 ) (modified from Winnepenninckx et al., 1995) and 2023R (5 0 -GGTTCACCTACGRAAA-3 0 ) (modified from Pradillon et al., 2007) using the Phusion Ò DNA polymerase (Finnzymes, Finland). Cycling conditions were as follows: initial denaturation at 96 C for 5 min, followed by 35 cycles of 96 C for 1 min, 55 C for 1.5 min, 72 C for 2 min and final elongation at 72 C for 10 min. The purified PCR products (PCR Purification Kit; Qiagen, Hilden, Germany) were sequenced bidirectionally with the PCR primers and an internal reverse primer (5 0 -CAGA-CAAATCGCTCC-3 0 ) 1272 nucleotides downstream of the 1F primer. All sequencing reactions were performed with an ABI PRISM 3100 genetic analyser (Applied Biosystems, Foster City, CA, USA).
The generated 18S rRNA gene sequences and all sequences from the Stilbonematinae available in Gen-Bank, as well as four Draconematinae sequences as outgroup were aligned using MAFFT Q-INS-I, which considers the predicted secondary structure of the RNA for the alignment (Katoh et al., 2005). Alignments were manually inspected and 5 0 and 3 0 end-trimmed using Geneious software version 6 (Drummond et al., 2011). The optimal substitution model was assessed using the Akaike information criterion as implemented in MEGA 5.3 (Tamura et al., 2011) and the GTRCGCI model was chosen. Phylogenetic trees were reconstructed using maximum likelihood-(RAxML) (Stamatakis, 2006) and Bayesian inference-based (MrBayes) (Ronquist & Huelsenbeck, 2003) methods. The dataset was screened for chimeric sequences by calculating maximum likelihoodbased trees on three partitions of the alignments (0À600 bp, 601À1200 bp and 1201À1799 bp). The Stilbonematinae sequences Y16915 (designated Eubostrichus dianae) and Y16921 (designated Robbea hypermnestra) had statistically supported positions in 2 (Y16915) and 3 (Y16921) different genus level clades across all three partitions and were excluded from the final analysis. MrBayes was run for four Mio generations using four chains. Convergence was evaluated by plotting the generations versus logL and the burn-in was set to 1 Mio generations. Node stability was evaluated using posterior probabilities (pp, Bayesian inference) and bootstrap support (200 RAxML rapid bootstrap runs, maximum likelihood) (Stamatakis et al., 2008) with values above .80 considered significant.

Results
Class Chromadorea Inglis, 1983Subclass Chromadoria Pearse, 1942Order Desmodorida De Coninck, 1965Suborder Desmodorina De Coninck, 1965Superfamily Desmodoroidea Filipjev, 1922Family Desmodoridae Filipjev, 1922Subfamily Stilbonematinae Chitwood, 1936Robbea Gerlach 1956 Diagnosis. (Modified from Tchesunov, 2013): Stilbonematinae. Cuticle transversely striated, except for the head region and the tip of the tail. Cephalic capsule when present with a block-layer; cephalic setae as long or longer than the subcephalic setae, usually directed straight forward. Amphidial fovea well developed, spirally coiled or loop-shaped. Pharynx distinctly tripartite, corpus muscular and clearly set off from the narrow isthmus. Pharynx bulbus largely glandular. Gubernaculum variable in shape, with or without dorso-caudal apophysis. The males of R. tenax Gerlach 1963 and the three new species have cup-shaped ventral papillae in the post-pharyngeal region. These species share also a stout body with a not exceeding 100. The species R. gerlachi Boucher 1975 from which only a female has been described may also belong to this group. Symbiotic bacteria rod shaped, corn-kernel shaped or coccoid, usually covering the body as a monolayer.
Additional material. Several specimens in the author's collection and those used for SEM.
The sequences of the 18S rRNA gene are available from GenBank and have the accession numbers KJ414466-7.
Type locality. Subtidal sand bar in 10À50 cm depth at the north end of Carrie Bow Cay, Belize in coarse, poorly sorted calcareous sand with rubble ( Fig. 1).
Distribution. This robust species is extremely common in the above sand bar (compare Ott & Novak, 1989) and a similar sand bar at the south end of neighbouring South Water Cay.
Other localities are open sand patches between Thalassia testudinum beds and patch reefs to the west and north of Carrie Bow Cay in up to 1.5 m depth and medium, poorly sorted sand at the base of 'sand bores' south of Carrie Bow Cay in 5À6 m depth (Fig. 1).
Etymology. Named after Hypermnestra, the only one of the 50 daughters of the king of Argos, Danaos, who did not kill her husband in the wedding night as requested by her father although she was armed with a dagger. This refers to the presence of a spiculum in females (intersexes).

Description
Body cylindrical (Figs 2, 3), head diameter at level of cephalic setae 20À23 mm, diameter at level of posterior margin of amphidial fovea 40À54 mm in males, 33À42 mm in females, at end of pharynx 47À55 mm, maximum body diameter 55À82 mm, anal diameter 55À68 mm in males, 50À60 mm in females. Tail conical, 140À174 mm long in males and 120À150 mm in females (Figs 7,8).
Cuticle finely transversely striated except for the first 28À36 mm of the head (Figs 5À8, 14) and the last 35À50 mm of the tail (Fig. 15), annules 0.45 to 0.55 mm wide (18À22 annuli/10 mm); non-striated head cuticle reinforced (cephalic capsule). The anteriormost circle of head sensillae (inner labial sensillae) is represented by 6 fingerlike papillae, 2 mm long in lateral, subventral and subdorsal position surrounding the mouth opening ( Fig. 19). The second circle consists of 6 short outer labial sensillae, 3À6 mm long, on the margin of the membranous buccal field; 4 cephalic setae flanking the anterior margin of the amphidial fovea, 32À38 mm long; 3 circles of 8 subcephalic setae each, 28À38 mm long, on the non-striated part of the head region, the posteriormost at the level of the posterior margin of the amphidial fovea (Figs 11, 17); 8 rows of somatic setae along the whole length of the body, 20À35 mm long somatic setae alternating with only 5À12 mm long bristles (Fig. 4). In males, there is a distinct field of about 7À10 stout, conical setae, 5 mm long on the ventral side in front of the cloacal opening (Figs 8,25,26). Non-striated part of the tail in females with 2 pairs of stout setae, the first pair 7 mm long, shortly behind the end of the annulation; the second pair of 3À4 mm long, close to the tip of the tail (Fig. 15); in males with a small velum ventrally (Fig. 23). Three caudal glands with separate openings at the tail tip ( Fig. 16). Males have a 380À450 mm long row of 16À19 (mean 17.2, n D 16) conspicuous cuticular papillae along the mid-ventral line beginning at a distance of 65À95 mm from the anterior end at the level of the posterior bulbus of the pharynx (Figs 4,20). Papillae cup-shaped, with a diameter of 11À15 mm, on short annulated stalks, bearing a conical seta in its centre (Figs 21,22). Amphidial foveas situated at the anterior end bordering the buccal field, showing a distinct sexual dimorphism: small, slightly oval spirals with 1.25 turns in females, 13À18 mm long and 9À12 mm wide; in males much larger, elongated loops, 28À38 mm long and 16À20 mm wide (Figs 5,6,12,18,28,29).
Pharynx (Figs 4,27) 90À117 mm long, with a minute tubular buccal cavity, 7À12 mm long and 4 mm in diameter, leading into a conspicuous pyriform muscular corpus, 34À40 mm long and 18À25 mm wide, which is clearly set off from the following 32À40 mm long isthmus. Posterior bulbus subspherical, 20À38 mm long, 30À45 mm wide, largely glandular, containing only weak muscles. No cardia.
Nerve ring 50À65 mm from anterior end; no secretoryexcretory pore or ventral gland seen; 8 rows of glandular sense organs (two in each lateral and each median line) connect to the small somatic setae.
Females didelphic, ovaries reflexed, long uteri leading to a strongly cuticularized vagina; vulva (Fig. 13) at 41À45% of body length. Large eggs (190 £ 50 mm) are often present in one or both uteri, which also contain usually many sperm cells of the same shape and size as seen in males. All females are intersexes, having spicula and a gubernaculum in the anal region (Figs 7,9,30). Spicula and gubernaculum much smaller and of simpler construction than in males: length of the female spicula, 53À60 mm (chord) or 62À70 mm (arc); gubernaculum, 10À15 mm long with a similar sized apophysis. No traces of other parts of a male genital apparatus were found. A weakly cuticularized vulva primordium but no other female or male characteristic features could be seen in 7 of 11 large (probably 4th stage) juveniles.
The bacteria covering the cuticle of the worms (Fig. 14) are distributed over the whole body, leaving only the tip of the tail and the cephalic capsule uncovered. On the ventral side the bacteria rarely reach the basis of the cephalic capsule. The bacteria are rod shaped and are usually attached with their longitudinal axis perpendicular to the cuticle surface. In a few cases, however, bacteria were seen lying parallel to the cuticular annuli of the worm (Fig. 14). The thiotrophic bacterial ectosymbionts have been previously characterized (Bayer et al., 2009) and belong to the MONTS clade of Gammaproteobacteria  that also contains the symbionts of the mouthless nematode genus Astomonema and of gutless oligochaetes. The GenBank accession number for their 16S rRNA gene is EU711428.
Diagnosis. Species with a distinct cephalic capsule with block-layer; 16À19 cup-shaped stalked ventral papillae in the post pharyngeal region; pharynx almost equally divided into corpus, isthmus and bulbus; all females are intersexes; spicula weakly cephalate, gubernaculum with dorsocaudal apophysis, pronounced sexual dimorphism of the amphidial fovea. Symbiotic bacteria rod-shaped. Measurements. See Table 2.
Additional material. Several specimens in the authors' collection and those used for SEM. The sequence of the 18S rRNA gene is available from GenBank and has the accession number KJ414465.
Etymology. Named in honour of Klaus Ruetzler, CCRE programme director, friend and generous host on Carrie Bow Cay.

Description
Body slender, cylindrical (Fig. 32), head diameter at level of cephalic setae 20À25 mm, diameter at level of posterior margin of amphidial fovea 25À30 mm at end of pharynx 33À39 mm, maximum body diameter 40À60 mm, anal diameter 32À45 mm. Tail conical, 60À88 mm long (Figs  35, 36). Cuticle finely transversely striated except for the first 32À44 mm of the head and the last 30À42 mm of the tail, annules 0.5À0.65 mm wide (15À20 annuli/10 mm) (Figs 39, 42); head with a circle of 6 finger-like inner labial papillae surrounding the mouth opening (Fig. 38); 6 short outer labial sensillae, 2.5À4 mm long, at the margin of the membranous buccal field; 4 cephalic setae, flanking the anterior margin of the amphidial fovea, 21À27 mm long (Figs 33, 34, 37); a first circle of 8 subcephalic setae at the level of the amphidial fovea, a second circle approximately in the middle, a third circle at the posterior margin of the cephalic capsule; subcephalic setae, 18À27 mm long, 8 rows of somatic setae along the whole length of the body. Somatic setae of males in the region of the row of ventral papillae stout, 12À17 mm long, the following body setae thinner and shorter (8À13 mm). There is a transverse row of precloacal setae a short distance anterior to the cloacal opening (Figs 36,42,43). Somatic setae of females in cervical region 12À15 mm long, the following body setae 17À20 mm long. Males with a 250À320 mm long row of 15À17 conspicuous mid-ventral cuticularized papillae (Figs 34, 40), first papilla situated a short distance posterior to the end of the pharynx. Papillae with short annulated stalks, bearing central conical setae (Fig. 41). The non-striated part of the tail bears no terminal setae, in males there is a velum present (Figs 42, 44). Loop-shaped amphidial foveas (7À12 mm long, 10À12 mm wide), situated at the anterior end bordering the buccal field, with only slight sexual dimorphism. Pharynx 72À90 mm long; minute tubular buccal cavity, 10À15 mm long and 2À4 mm in diameter, leading into a conspicuous pyriform muscular corpus, 22À30 mm long and 16À18 mm wide, clearly set off from the following 24À41 mm long isthmus. Spherical bulb, 12À18 mm long and 17À20 mm wide, mainly glandular and containing only weak muscles. No cardia (Figs 33,34,45).
Nerve ring 42À60 mm from anterior end; no secretoryexcretory pore or ventral gland seen; there are 8 rows of glandular sense organs (two in each lateral and each median line) connecting to the small somatic setae.
A dense monolayer of rod-shaped symbiotic bacteria (Fig. 39) covers almost the whole body, beginning at the level of the pharyngeal terminal bulb or at the level of the posterior region of the cup-shaped cervical papillae (Fig. 46) and terminating with the cuticle striation at the tail tip or already at the level of the cloacal opening. Rows or plaques of smaller cocci stretch forward up to the anterior bulbus of the pharynx, but not up to the non-striated part of the head.   Additional material. Several specimens in the authors' collection and those used for SEM.
Measurements. See Table 3. The sequence of the 18S rRNA gene is available from GenBank and has the accession number KJ414464.
Distribution. Regularly in fine sand samples at several locations in the vicinity of the CBC laboratory (Fig. 1).

Description
Body slender, cylindrical (Figs 48,49), tapering only slightly towards anterior end, head diameter at level of cephalic setae 18À25 mm, diameter at level of posterior margin of amphidial fovea 20À29 mm, at end of pharynx 25À33 mm, maximum body diameter 33À42 mm, anal diameter 24À35 mm. Tail conical, 70À105 mm long; non-striated tail tip 17À19 mm long in males and 23À30 mm in females (Figs 52, 53, 62). Cuticle finely transversely striated except 24À28 mm of the anterior part of the head (cephalic capsule) and the tail tip, annules 0.6 to 0.7 mm wide (14À17 annuli/10 mm) (Fig. 57). Mouth opening surrounded by 6 finger-like inner labial papillae in lateral, subventral and subdorsal position (Fig. 56) followed by a circle of 6 short labial sensillae, 3À4 mm long, surrounding the membranous buccal field; a circle of 4 cephalic setae flanking the anterior margin of the amphidial fovea, 20À22 mm long; closely followed by a circle of 8 subcephalic setae, 16À26 mm long, a second circle near the end of the cephalic capsule (Figs 50, 51, 55); 8 rows of somatic setae along the whole length of the body, 5À8 mm long; a pair of 6À8 mm long setae at the begin of the non-striated part of the tail. No special precloacal setae. Mid-ventral line of males with a 150À170 mm long row of 8À9 conspicuous cup-shaped papillae (diameter 7 mm) positioned on short stalks. Papillae with central setae (1.5 mm long) (Figs 51, 59, 60). The first of these papillae is situated at a distance of 70À110 mm from the anterior end at the level of the pharyngeal terminal bulb. Amphidial foveas spiral with 2.5 turns, slight sexual dimorphism: in females oval, 12À13 mm long and 8À12 mm wide, situated directly at the anterior end bordering the buccal field; in males larger, somewhat elongated, 15À16 mm long, 12À14 mm wide, 1À4 mm from the anterior end (Figs 50,  51, 64).
A minute tubular buccal cavity, 7À10 mm long and 2À3 mm in diameter, leading into a tripartite, 60À95 mm long pharynx consisting of an anterior pyriform muscular corpus (19À30 mm long, 12À18 mm wide), the following isthmus (30À50 mm long) and the terminal bulb (12À18 mm long, 17À20 mm wide). Terminal bulb largely glandular, containing only weak muscles. No cardia (Figs 50,51,63,69).
Nerve ring 44À67 mm from anterior end; no secretoryexcretory pore or ventral gland seen; 8 rows of glandular sense organs (two in each lateral and each median line) connecting to the small somatic setae.
Females didelphic, ovaries reflexed, long uteri leading to the vagina, ventral gso enlarged in the region of the uteri; vulva at 52À55% of body length.
Epigrowth of symbiotic bacteria starting at a defined line at a distance from the anterior end (Figs 66, 67) showing a sexual dimorphism: in males the bacterial coat begins at 220À340 mm (2.7À3.5 pharynx length), in females at 155À195 mm (2À2.3 pharynx length). A monolayer of corn-kernel shaped bacteria (1.5 £ 0.8 mm) (Figs 57, 58) covers the remaining body except the nonstriated tip of the tail. At the start of the coat the body diameter abruptly becomes smaller to accommodate the thickness of the bacterial layer without increasing the consortium's diameter (Fig. 67). In some specimens patches of bacteria occur around the cup-shaped papillae; here rods lie parallel to the cuticle surface (Fig. 61). Occasionally larger coccoid bacteria are found on the normally symbiont-free anterior body part (Fig. 68). The role of these bacteria is unknown.
Suctorians are frequently attached to the cuticle in the posterior body region (Fig. 49). Diagnosis. Species with indistinct cephalic capsule; 8À9 cup-shaped ventral papillae in post-pharyngeal region; isthmus occupies more than 50% of pharynx length; spicula cephalate with velum, gubernaculums with dorso-caudal apophysis ending in a spherical swelling; amphidial fovea spiral in both sexes, larger in males. Symbiotic bacteria corn-kernel shaped, bacterial coat starting at a defined line 2 to 3.5 pharynx lengths from the anterior end.

Discussion
Systematics Gerlach (1956) erected the genus Robbea for a male animal collected on the coast of Brazil, which he named Robbea caelestis. A second species was subsequently described from the Maldive Islands by the same author, Robbea tenax Gerlach (1963). The material consisted of one female and three males, the latter showing conspicuous cervical papillae. Both Robbea species were later placed into the genus Catanema Cobb, 1920 by Platt & Zhang (1982). Recently Tchesunov (2013) proposed diagnoses for both Robbea and Catanema in which the major distinguishing character is the shape of the amphidial fovea, which is distinct in Robbea but is reduced to a small apical opening in Catanema. Other characters such as the degree to which the pharyngeal corpus is set off from the isthmus, 'clearly' in Robbea, 'distinctly' in Catanema (see Fig. 70 for the situation in Robbea hypermnestra), and the presence (Robbea) or absence (Catanema) of a cephalic capsule are less clear and not all descriptions published so far contain explicit statements regarding these characters. According to Tchesunov's criteria, the following species belong to the genus Robbea: R. caelestis   Hopper & Cefalu (1973) described as R. tenax from Florida and the three new species described herein. R. gerlachi Boucher 1975 has only been described from a female, but according to the shape of its amphidial fovea it should be placed into the genus Robbea. One additional species of Robbea has been suggested by Tchesunov (2013), but due to the lack of male specimens the species has not been described in detail yet.
To consolidate the taxonomic descriptions of the three newly described Robbea species on a molecular level we used phylogenetic 18S rRNA analyses. The tree shows a clear separation between the clade containing the three new Robbea species and the other Stilbonematinae (Fig. 71). We also provide additional 18S rRNA gene sequences for the genera Catanema and Leptonemella that only had a single 18S rRNA gene deposited prior to this study. The morphological characters of the three yet undescribed Catanema species included in the phylogenetic analyses conform to the diagnosis given by Tchesunov (2013). Our data confirm that both Robbea and Catanema, as well as all other genera of Stilbonematinae with available sequence data are represented by statistically supported genus level clades in 18S rRNA gene based phylogenetic analyses. This high resolution of the 18S rRNA gene finally allows to assign or to re-evaluate the correct taxonomic affiliation at the genus level for new or already deposited sequences.
For the moment this ends the confusion around these two genera to which we have added by assigning two stilbonematine species to the genus Robbea (namely Robbea sp. 1 and 2) in Bayer et al. (2009). We have reinvestigated the nematode material used in that paper where possible. In the case of Robbea sp. 1 from Calvi (Corse), there obviously had been a mix-up during the sample sorting, and two different nematode species were present in the sample analysed: a Laxus sp. that is morphologically indistinguishable from Laxus cosmopolitus described from the Adriatic  and a yet undescribed Catanema species. Judging from the phylogenetic position of the deposited 18S rRNA gene, a specimen of the former species was probably sequenced. Robbea sp. 2 from the Cayman Islands is a member of the Catanema clade (Fig. 71). Robbea sp. 3 is morphologically and phylogenetically identical to the newly sequenced Robbea hypermnestra sp. nov. specimens (Fig. 71). The 18S sequences for Robbea hypermnestra sensu Kampfer 1998 (nomen nudum) that were formerly deposited under Y19621 were identified as chimeric but the last 600 bp are identical to the correct Robbea hypermnestra sp. nov. sequences from this study as well as to the Robbea sp. 3 sequence published by Bayer et al. (2009). The sequences in the Kampfer et al. (1998) paper were generated from pools of up to 50 nematodes that likely were contaminated by other nematode species. Thus, the chimeric nature of the published sequences could likely be attributed to different priming bias in the forward and reverse primers used. In contrast, all sequences generated in the present work come from single nematode specimens. This has been made possible with high-yield DNA extraction methods based on Gen-eReleaser (Bioventures) (Schizas et al., 1997) or the Blood and Tissue kit (Qiagen) combined with highly sensitive and efficient polymerases such as the Phusion Ò DNA polymerase (Finnzymes). Successful PCR based sequencing of multiple genes from the DNA of a single meiofauna individual has been performed without amplification using specimens as small as 500 mm long microturbellarians . We thus emphasize that, wherever possible, single individuals should be used for PCR-based gene assays. While the diagnoses given by Tchesunov (2013) currently hold true for the genera Robbea and Catanema, they are based on characters such as the reduction of the amphidial foveas. However, these characters are also present in some species of other genera, such as Stilbonema and Leptonemella (e.g. in the type species L. cincta COBB 1920). Findings of new species may make emendation of diagnoses that were based on morphology alone necessary. Our results clearly indicate the necessity to provide molecular data to confirm the morphological identification and that larger taxon sampling is an important factor to be able to validate sequencing results and enable for example barcoding approaches.

Intersexes
Cases of intersexuality have been described from various terrestrial, parasitic or marine nematodes (e.g. Zhuo et al., 2009;Moura et al., 2014). In the latter, intersexes are most commonly reported as females with a functional reproductive system and rudimentary male sexual characters (e.g. Gourbault & Vincx, 1990;Riemann et al., 2003;Zhuo et al., 2009;Miljutina et al., 2013;Moura et al., 2014), just as observed in our newly described R. hypermnestra. However, only few intersex individuals are usually found within nematode populations (Gourbault & Vincx, 1990). As driving factor for intersexuality in nematodes, unfavourable environmental conditions (Davide & Triantaphyllou, 1968), hybridization between closely related species (Steiner, 1923;Krall, 1972) or genetic or chromosomal disorder (Roy & Gupta, 1975;Jairajpuri et al., 1977) have been hypothesized. Considering the clean and stable environment around Carrie Bow Cay and the fact that all females of R. hypermnestra are intersexes, environmental sex determination appears unlikely. We cannot exclude hybridization events with closely related species in the past. It is difficult to assign any specific function to the spicula observed in R. hypermnestra intersexes and the R. hypermnestra chromosome set has not been determined, thus our hypotheses will remain rather speculative. The spicula could be remnants from a previously hermaphroditic lifestyle, but since no hermaphroditic lifestyle is known from stilbonematine nematodes, the interpretations that R. hypermnestra females are in a transition to a hermaphroditic stage, is more likely. In fact, individuals of the wellstudied terrestrial nematodes C. elegans and C. briggsae are either male or hermaphrodites where the hermaphrodites are descendants from male or female ancestors and mutations in two independent pathways were sufficient for C. elegans to develop self-fertile hermaphrodites (Baldi et al., 2009). This indicates a high plasticity and flexibility in the expression of sexual phenotypes in nematodes in general. Hermaphroditism could be favourable at low effective population densities to ensure reproduction in the absence of a mating partner (Pires-DaSilva, 2007). To test whether this is the case, the distribution patterns and gender ratios of the R. hypermnestra populations need to be monitored systematically.

Ecological notes
The shallow subtidal sands around Carrie Bow Cay harbour a diverse interstitial meiofauna. Among these are representatives of several taxa known to harbour bacterial symbionts. These include the ciliate genus Kentrophoros, the mouthless nematode genus Astomonema (authors' unpublished observation), several species of the mouthless catenulid flatworm genus Paracatenula Gruber-Vodicka et al., 2011) and the gutless marine oligochaete genera Inanidrilus and Olavius (Ers eus, 1990). So far, stilbonematid nematode species of several genera have been described from shallow water habitats around Carrie Bow Cay. These include Laxus oneistus, Stilbonema majum, Adelphus rolandi, Eubostrichus dianae, E.parasitiferus and E. fertilis (Ott et al., , 2014Ott, 1997;Kampfer et al., 1998;Polz et al., 1999). We add to this diversity with our description of three new Robbea species that live in vicinity of Carrie Bow Cay (Fig. 1). Available physiological and molecular evidence shows that all (Paracatenula, Stilbonematinae) or the majority of bacterial symbionts (oligochaetes) are sulphur-oxidizing chemoautotrophs (SOBs) (Dubilier et al., 2006;Bayer et al., 2009;Gruber-Vodicka et al., 2011). Associations of SOBs with motile hosts appear to be beneficial when oxygen-containing surface layers are spatially separated from sulphidic deeper layers and vertical migration by the host can alternately supply the symbiotic bacteria with sulphide and oxygen (Giere et al., 1991;Ott et al., 1991).
Shallow sandbars in the immediate back reef area of Carrie Bow Cay consist of coarse to medium, unsorted carbonate sand of local origin that has been deposited under sheltered conditions and has a spacious interstitium with reduced, sulphidic conditions in layers several centimetres below the sediment surface (Ott & Novak, 1989). Here large species of Stilbonematinae (Laxus oneistus, Stilbonema majum) and also R. hypermnestra are found in high densities (Ott & Novak, 1989). In contrast, a diverse assemblage of smaller Stilbonematinae species such as E. dianae, E. fertilis, R. agricola and R. ruetzleri inhabit the fine sands in the vicinity of lagunal mangrove islands and Thalassia testudinum seagrass beds (Ott et al., 2014). Additionally to the described species, several undescribed species and possibly genera of stilbonematine nematodes can be found in the shallow water habitats in the vicinity of Carrie Bow Cay (authors' pers. observation).