An Overview of Y-Family DNA Polymerases and a Case Study of Human DNA Polymerase η

Y-Family DNA polymerases specialize in translesion synthesis, bypassing damaged bases that would otherwise block the normal progression of replication forks. Y-Family polymerases have unique structural features that allow them to bind damaged DNA and use a modified template base to direct nucleotide incorporation. Each Y-Family polymerase is unique and has different preferences for lesions to bypass and for dNTPs to incorporate. Y-Family polymerases are also characterized by a low catalytic efficiency, a low processivity, and a low fidelity on normal DNA. Recruitment of these specialized polymerases to replication forks is therefore regulated. The catalytic center of the Y-Family polymerases is highly conserved and homologous to that of high-fidelity and high-processivity DNA replicases. In this review, structural differences between Y-Family and A- and B-Family polymerases are compared and correlated with their functional differences. A time-resolved X-ray crystallographic study of the DNA synthesis reaction catalyzed by the Y-Family DNA polymerase human polymerase η revealed transient elements that led to the nucleotidyl-transfer reaction.

R eplication of genomic DNA is a prerequisite for cell proliferation. High-fidelity DNA polymerases, regardless of their diversity in amino acid sequence, three-dimensional structure, origin, or complexity of subunit composition, are uniformly accurate and processive, such that up to billions of Watson−Crick (WC) base pairs are copied rapidly with high fidelity. 1−3 However, these remarkable high-speed and highaccuracy machines have an Achilles heel that is the intolerance of any alteration in the four normal bases, A, G, T, and C. The presence of a chemically modified DNA base or a DNA strand break could cause stalling or collapse of a replication fork. Because genomic DNA is frequently damaged by environmental toxins, radiation, and endogenous and exogenous metabolites, 4 every living organism relies on two general pathways to cope with "road blocks" in DNA replication. 5,6 One is to use specialized translesion DNA polymerases to accommodate DNA base lesions and continue DNA synthesis past damaged sites. 7−9 Alternatively, a detour by the homologous recombination pathway can avoid troublesome lesion sites and allow replication to proceed. 10,11 DNA base lesions cause structural and chemical changes at the site of damage and inevitably hinder normal base pairing and base stacking in the vicinity, hence leading to general instability and distortion of the DNA double helix. 12−14 As a result, it is suggested that two different specialized polymerases are required to bypass a lesion in translesion DNA synthesis (TLS). 15 The first polymerase accommodates the damaged base and performs nucleotide incorporation directly opposite the lesion; the second polymerase extends the DNA primer beyond the lesion for several base pairs before the damaged DNA can be safely handled by a replicase (defined as a highfidelity and high-processivity polymerase dedicated to the replication of normal DNA genomes). Y-Family polymerases are generally employed in the first step of TLS. For the second step of TLS, Escherichia coli DNA pol II and eukaryotic pol ζ, both of which belong to the B-Family, have been shown to perform primer extension after a lesion or a mismatched base pair. 16−18 Because DNA lesions are varied in shape and chemical nature, a large fraction of DNA polymerases in each organism are specialized for repair and translesion synthesis, while replicases that copy genomic DNA of uniform WC base pairs consist of a relatively small fraction of polymerases in the genome. In E. coli, only one of the five total DNA polymerases is the high-fidelity and high-processivity replicase (pol III), 19 and the most abundant pol I has high fidelity, but low processivity, and performs short patch synthesis in DNA repair. 20 The remaining three, the Y-Family pol IV and pol V and the B-Family pol II, are involved in translesion and mutagenic DNA synthesis. 3,21−23 In humans, only five of 17 DNA polymerases are involved in normal genomic and mitochondrial DNA replication (pol α, δ, ε, and γ and telomerase). Of the remaining 12, four are Y-Family TLS polymerases [pol η, ι, κ, and Rev1 (see details below)], and eight others (pol β, λ, and μ, TdT, pol ν, θ, and ζ, and PrimPol) are required for TLS and repair of DNA breaks. 24−34 Individual Y-Family polymerases have different template base-binding sites and different preferences for the incoming nucleotide. 8,35 The general properties of Y-Family DNA polymerases are (1) the ability to conduct translesion synthesis with good accuracy, (2) a high error rate when copying normal DNA, (3) a lack of the 3′−5′ exonuclease activity and intrinsic proofreading ability, 36 (4) a low catalytic efficiency, and (5) a low processivity compared to that of DNA replicases. 9 The last two seemingly undesirable features ensure that they do not associate with or stay on a replication fork for more than several base pairs, which is thus advantageous in the scheme of DNA replication. The Y-Family polymerases are recruited to replication forks only when necessary. Regulation of their recruitment will be summarized in this review. For more comprehensive treatments, readers are referred to two recent reviews. 9,28 The focus of this review will be on the catalytic properties of Y-Family polymerases and comparisons with those of replicases.
Y-Family polymerases are divided into six major groups based on amino acid sequence. They are represented by E. coli pol IV (known as DinB) and pol V (of which UmuC is the catalytic subunit) and four human enzymes, pol η, ι, κ, and Rev1 7,9 ( Figure 1A). All Y-Family polymerases comprise two functional parts, the polymerase catalytic region of 350−500 residues and a regulatory region from 10 (as in DinB, Dbh, and Dpo4) to 600 residues (as in Rev1).
Among these six groups, bacterial DinB and its archaeal homologues, Dpo4 and Dbh, are related to eukaryotic pol κ by sharing a structural gap in the catalytic region ( Figure 2) and their tendency to make deletion mutation and ability to bypass bulky DNA adducts 8,37−39 (see details below). Initially, pol η and ι were considered close homologues, 40 but the two enzymes turn out to share little functional similarity. 9,41 Inactivation of POLH (the gene encoding pol η) in humans leads to extreme UV sensitivity and predisposition to skin cancers, and the genetic disease is known as the variant form of xeroderma pigmentosum (XPV); 42,43 however, the function of pol ι remains uncertain as Poli −/− and Poli +/+ mice are alike in all aspects of growth. 44 As the tenets of evolution would predict, the substrate specificity and prevalence of Y-Family polymerases are highly correlated with the abundance of naturally occurring DNA lesions. Ultraviolet radiation (UV) is an intrinsic part of sunlight, and UV-induced cis-syn pyrimidine dimers (cyclobutane pyrimidine dimers, or CPD) and 6-4 photoproducts are the oldest and most prevalent DNA lesions on earth. 45 Accordingly, UmuC, ubiquitous in bacteria, and pol η, found in all eukaryotes, primarily bypass UV-induced CPD lesions. 42,43,46 Because 6-4 photoproducts are efficiently removed by nucleotide excision repair, they are rarely encountered in the S phase. In addition, Rev1, which was originally identified and isolated because of its UV-induced expression and UV sensitivity in its absence, 47 is present universally among eukaryotes. Rev1 is a template-independent deoxycytidyl transferase and has more than 600 residues outside of the catalytic region (Figure 1). 48 The noncatalytic regions of Rev1 appear to regulate other Y-Family polymerases and contribute to TLS more than its catalytic activity. 49−52 Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants produced by incomplete combustion of fossil fuels and biomass, and they are also present in tobacco smoke and   the human diet. 53 PAHs can form DNA base adducts, for example, benzo[a]pyrene, one of the earliest identified carcinogens. 54 Dpo4 and eukaryotic pol κ in particular are specialized to bypass these DNA bulky adducts. 37,55−61 Their close relative E. coli pol IV (DinB) bypasses N2-furfuryl-dG adducts efficiently. 62 Interestingly, the absence of pol κ in cell cultures also causes mild UV sensitivity, and pol κ has been shown to participate in excision repair of UV lesions. 63,64 In addition, 7,8-dihydro-8-oxyguanine (8-oxo-G) is a major oxidative lesion and promutagenic. Replicative DNA polymerases tend to incorporate dATP opposite an 8-oxo-G, resulting in a G → T transversion mutation. 65 Dpo4, pol η and ι, and presumably Rev1 are able to bypass 8-oxo-G with correct dCTP incorporation 66−71 (Table 1).
Regulatory regions of Y-Family polymerases often contain a PCNA interacting peptide (PIP), a Rev1 interacting region (RIR), a ubiquitin-binding module (UBM), and a ubiquitinbinding zinc domain (UBZ) 8,9 (Figure 1). Rev1 is unique and has an N-terminal BRCT domain and a C-terminal proteinbinding domain (Figure 1). Structural studies of these regulatory modules are mostly conducted by NMR as summarized in Table 1 and shown in Figure 3. Every Y-Family polymerase interacts with the replication processivity cofactor, β-sliding clamp in bacteria and PCNA in archaea and eukaryotes, via its PIP or BRCT domain (Rev1). 72,73 When DNA is damaged, PCNA becomes ubiquitinated, 74,75 and each eukaryotic Y-Family polymerase uses UBM or UBZ to interact with ubiquitin. In addition, human Y-Family polymerases η, ι, and κ each has an RIR that binds the C-terminal domain of Human pol η, whose inactivation directly leads to cancer, 79−82 is among the most scrutinized. In addition to the regulatory elements listed above, pol η itself can be ubiquitinated on a Lys residue within the nuclear localization signal (NLS) segment ( Figure 3A), and this modification prevents the nearby PIP from interacting with PCNA 83 ( Figure  1B). Pol η is also phosphorylated by ATR kinase upon UV irradiation ( Figure 3A), which allows its TLS activity and checkpoint response to UV damage. 84 The Y-Family polymerase catalytic region is composed of a conserved catalytic core, which includes the finger, palm, and thumb subdomains, and an appendage of the little finger domain. 8 The palm, finger, and thumb subdomains are found in all DNA polymerases. The "little finger" domain (LF), also known as the polymerase-associated domain (PAD), 85 is unique to the Y-Family and has more sequence variability than the catalytic core. 8,86 Three-dimensional structures of the catalytic region of a number of Y-Family polymerases from bacteria and archaea to humans have been reported (Table 1 and references therein). DNA substrate is bound between the thumb and LF. The palm subdomain contains the catalytic carboxylates that coordinate two Mg 2+ ions, and the finger subdomain interacts with the template base and the incoming nucleotide ( Figure 3).
In DNA polymerases other than the Y-Family members, the finger subdomain undergoes a large conformational change from an open state represented by apoproteins to a closed state adopted by the catalysis ready enzyme−DNA−dNTP ternary complexes 87−92 (Figure 4A,B). In contrast, the catalytic core of Y-Family polymerases contains a preformed active site with the finger closed in the absence of DNA, dNTP substrate, or both 85,93−95 ( Figure 4C,D). Some Y-Family members can bind an incoming dNTP even in the absence of a base pair partner (templating base) and base stacking with the primer end. 96 Correlated with being preformed, the active site of Y-Family members is large and solvent-exposed 8,67,97 ( Figure 5). Thus, in a complete cycle of primer extension, from diffusion-in of dNTP and Mg 2+ ions and the nucleotidyl-transfer reaction to pyrophosphate release and DNA translocation, no significant domain movement is needed or observed. 98−100 In contrast, the closed catalytic center of a replicative polymerase can accommodate only a WC base pair between a template base and an incoming dNTP and DNA and dNTP are constrained to the correct alignment for the nucleotidyl-transfer reaction. 88,101 The finger subdomain of a replicase has to open to release reaction product pyrophosphate and allow DNA to translocate and also a dNTP to bind for the next round of DNA synthesis ( Figure 4A,B). The perfect alignment of a normal DNA and a correct dNTP in the active site of a replicative polymerase underlies its high fidelity and high catalytic efficiency. In contrast, the loose fit of substrates in Y-Family polymerases leads to a low fidelity, a low catalytic efficiency, and a low processivity of DNA synthesis.
■ LARGE CONFORMATIONAL CHANGES OF DPO4, DBH, AND POL κ Substrate-dependent large conformational changes do occur in some Y-Family members. The catalytic core (CC) and little finger (LF) are connected by a flexible linker in all Y-Family polymerases. In the DinB subfamily, including Dpo4, Dbh, and pol κ, the CC and LF freely move relative to each other in the absence of DNA. 86,93,100,102 Large conformational changes are induced upon DNA binding. 86,100,103 Because of limited interactions between the CC and LF in Dpo4, Dbh, and pol κ, a large structural gap divides the CC and LF of these polymerases even in the ternary complexes with DNA and dNTP 8,97 (Figure 2). Pol κ depends on its N-terminal extension (N-clasp) to hold the CC and LF together 102,103 ( Figures 1A and 2). The function of the structural gap in Dpo4, Dbh, and pol κ appears to accommodate minor groove bulky PAH adducts for lesion bypass 37,104 or nucleotides looped out of a DNA template strand leading to a deletion frameshift. 38,39,105 Similarly, a large gap between the CC and LF of Rev1 is filled by the N-terminal extension from the catalytic core 106,107 (Figures 1A and 2). In human Rev1, the finger and thumb are connected by a long loop insertion, and the CC is ring-shaped and encircles the DNA substrate. 107 In pol η, however, there is no structural gap and extensive interactions between the LF and the catalytic core stabilize the entire polymerase region (Figure 2). As a result, the apo pol η and DNA-bound complex structures are superimposable. 67,85,97 The interactions between the CC and LF of pol η form a "molecular splint" to hold the template strand in the B-form even in the presence of a UV cross-linked cis-syn pyrimidine dimer. 97 With this "molecular splint", pol η is able to insert dAMPs across a cis-syn thymine dimer and also extend the primer for two more nucleotides, 97,108 so that the resulting TLS product can be utilized by a replicase to continue DNA synthesis.
The rate of DNA binding has not been reported for most of the Y-Family polymerases. 35 The poor affinity of Dbh for DNA 109 perhaps is due to its unique linker between the CC and LF 86 rather than the large conformational changes per se. It will be interesting to study whether the lack of CC−LF interaction in Dpo4 and pol κ slows DNA substrate binding compared to that of pol η, in which CC and LF do not undergo such conformational changes.

■ SUBSTRATE SELECTION AND LESION BYPASS SPECIFICITY
Since the structures of Dbh, Dpo4, and yeast pol η were determined 12 years ago, the unusually large and solventaccessible active sites of Y-Family polymerases have been thought to underlie promiscuous lesion and dNTP selection. This notion has been supported by Dpo4's ability to bind and bypass many lesions (Table 1). Despite a generally enlarged replicating base pair-binding site, each Y-Family polymerase differs significantly in the size and shape of template basebinding site and has a different lesion preference. Pol η accommodates and accurately bypasses cis-syn pyrimidine dimers and also a 1,2-intrastrand d(GpG)−cisplatin crosslink 67,97,98 ( Figure 5), but it is blocked by 6-4 photoproducts, BPDE−dG, and other bulky adducts. 110 Neither pol κ nor pol ι is capable of bypassing the 3′ base of a cross-linked base dimer. Likewise, only pol κ, and not pol η or pol ι, can accommodate the minor groove BPDE−dG adduct and accurately incorporates dCTP opposite the lesion. [55][56][57]59,60 To achieve this feat, pol κ depends on the flexibility between the catalytic core and little finger domain, the large structural gap, and its unique Nterminal extension (N-clasp). 103,104 A couple of Y-Family polymerases have a specific preference for the incorporation of incorrect nucleotides, and the resulting mutations are sequence-specific. Pol η depends on two uniquely conserved residues (Arg61 and Gln38 in humans) for the accurate bypass of cis-syn pyrimidine dimers. 97,111 However, these two residues also play a key role in the efficient misincorporation of dGTP opposite a dT, particularly when the mismatched dT:dGTP replicating base pair is immediately preceded by an AT base pair, 99 in the DNA sequence called the WA motif. The misincorporation of dGTP by human pol η at the WA motif in undamaged DNA contributes to somatic hypermutation in the normal development process of the adaptive immune system. 112−114 Rev1 uses an arginine side chain to form two hydrogen bonds with the base of a dCTP and to serve as a template for dCTP incorporation, while a DNA template base is flipped out of the active site. 106,107 The biological consequence of poly-dC synthesis remains unclear. In parallel, pol ι has a narrow binding site for the replicating base pair and thus favors the Hoogsteen base pair. 115,116 It is well-known that pol ι prefers to incorporate dGTP opposite template dT instead of the correct dATP. 40,117 However, crystal structures of pol ι show that an incoming dGTP and templating dT form neither a wobble nor a Hoogsteen base pair, 116,118 and the molecular mechanism of  All DNA polymerases, regardless of the structure and fidelity differences, catalyze the same nucleotidyl-transfer reaction, that is the formation of a new phosphodiester bond between the 3′-OH of a primer strand and the α-phosphate of an incoming dNTP, and concomitantly break the phosphodiester bond between the αand β-phosphates of dNTP ( Figure 6A). The reaction requires two Mg 2+ ions in addition to a DNA template and primer and a dNTP, 8,24,119 and polymerases lower the energy barrier for product formation. All DNA polymerases contain two to three conserved carboxylates in the catalytic center. The Mg 2+ ions neutralize the catalytic carboxylates and triphosphates of dNTP and facilitate the alignment of the substrates for the chemical reaction. The reaction is pHdependent and analogous to acid−base catalysis, in which the nucleophile (3′-OH) needs to be deprotonated and the leaving group (pyrophosphate) needs to be protonated. 120 However, different from the classic acid−base catalysis in which conserved protein side chains serve as the general base and acid to extract and donate protons, DNA and RNA syntheses require two Mg 2+ ions for the catalysis. 121,122 Chemical reactions are heterogeneous because enzyme molecules are usually not synchronized. In addition, large conformational changes of many polymerases associated with each catalytic cycle easily obscure the small changes of chemical bond formation. Therefore, structural analyses of DNA polymerases were limited to static states of a homogeneous population. Crystal structures of a variety of DNA polymerases in complex with DNA and dNTP substrate have been reported, 24−26,89,92,119,123−125 where the nucleotidyl-transfer reaction was prevented by a nonreactive substrate, an inactivated polymerase, or the replacement of Mg 2+ with Ca 2+ because Ca 2+ supports the formation of the enzyme−DNA− dNTP ternary complex but not the chemical reaction. 122 Crystal structures of polymerase−DNA product complexes are also available. However, how the actual nucleotidyl-transfer reaction takes place is still unclear.
In the course of our structural studies of pol η by X-ray crystallography, we noted that both substrate and product complexes of pol η coexisted in the same crystal lattice. Taking advantage of the absence of large conformational changes and slow reaction rates, we set out to record the course of the nucleotidyl-transer reaction catalyzed by pol η using timeresolved X-ray crystallography. To achieve this goal, it is necessary to turn off and turn on a chemical reaction at will. After our failed attempt to make a caged dNTP that can be activated by laser light, 126 we succeeded in growing crystals of native pol η with normal DNA and dNTP at pH 6.0, and with one Ca 2+ per complex, no DNA synthesis took place. 127 These crystals were soaked in stabilization buffers at pH 6.8− 7.2 and continued to diffract X-rays to better than 2.0 Å resolution. Addition of 1 mM Mg 2+ in the soaking buffer at pH 7.0 initiated the nucleotidyl-transfer reaction in crystallo. After Mg 2+ exposure, at roughly 40 s intervals crystals were flashcooled in liquid nitrogen to stop the chemical reaction, and the frozen samples at different reaction stages were analyzed by Xray crystallography.
We found that binding of two Mg 2+ ions was necessary to align the 3′-OH of the primer strand and the α-phosphate of dNTP ( Figure 6B). With a single Ca 2+ occupying the B metalbinding site, the 3′-OH together with the deoxyribose at the primer end was oriented away from the dNTP. After two Mg 2+ ions had bound (at 40 s), the fully occupied A-site Mg 2+ coordinated by the 3′-OH and the α-phosphate of dNTP led to the alignment of the reactants and tightening of the catalytic center. Interestingly, there was a delay between the presence of the well-aligned reactants and the formation of the new phosphodiester bond. The delay may correspond to a ratelimiting step observed in solution. 128 Because the chemical reaction of the trillions of polymerase molecules in each crystal took place stochastically and at a different rate, when the new bond became detectable after Mg 2+ exposure for 80 s, every crystal contained two mixed reaction species, the substrate and product ( Figure 6B). With a diffraction resolution of 1.5−2.0 Å, the structures and proportions (occupancies) of the two reaction states of each crystal were readily refined. As the reaction progressed, the level of substrates decreased and the products accumulated. At 200 s, the crystals contained roughly equal amounts of the substrate and product immediately before and after the nucleotidyltransfer reaction, and a composite of the two resembles the hypothesized pentacovalent phosphoryl-transfer reaction intermediate ( Figure 6C). After 250 s, the two species seemed to reach equilibrium, with 70% being the product.
Our time-resolved study has revealed two transient elements in the chemical reaction. A water molecule, which formed a hydrogen bond with the 3′-OH, appeared at 80 s when the new bond started to form and disappeared at 200 s before the product peaked. This transient water was suspected to deprotonate the 3′-OH, but after substantial investigation, we found that this transient water is not the nominal general base because it can be replaced by a 2′-OH at the primer end without reducing the reaction rate. 127 Instead, a native dNTP may directly contribute to deprotonation of the 3′-OH because in the ternary complex of a nonreactive incoming nucleotide (dNMPNPP) a similar water molecule is stably bound to the 3′-OH without a chemical consequence. 97 The largest positional change in the transition from substrate to product occurred at the α-phosphorus of the incoming nucleotide, which moved 1.4 Å, and not the nucleophile 3′-O atom. It appears that the 3′-OH and dNTP held together by the two Mg 2+ ions in the catalytic center may stochastically approach each other to be within such a critically short distance that the pK a of the 3′-OH is reduced for the proton to leave readily. The transient water may be simply recruited to carry the proton away. In the absence of this particular water, such as in the case of the ribonucleotide substitution at the primer end, the proton may leave via 2′-OH to another water molecule. An alternative path of deprotonation of the 3′-OH has been proposed in dynamic simulation studies of three other DNA polymerases, 60,129,130 in which the proton leaves via the water coordinated by metal ion A to the end with the γ-phosphate of dNTP.
Among the two-Mg 2+ -ion-dependent DNA polymerases, a conserved general base is absent, and there is likely no fixed path for the proton to depart. Instead, the two Mg 2+ ions coordinated by the catalytic carboxylates perfectly align the primer end of DNA and dNTP, and the resulting electrostatic environment, including the contribution by the dNTP itself, likely reduces the pK a of the 3′-OH for the nucleophilic attack to take place.
The second transient element observed in the in crystallo reaction catalyzed by pol η is a third Mg 2+ . The third Mg 2+ , which replaced the conserved Arg (Arg61) that neutralizes the dNTP, started to appear after 140 s when the products began to accumulate and its occupancy increased with an increase in the level of products ( Figure 6B). Obviously, the +2 charged Mg 2+ is more effective than the +1 charged Arg in neutralizing the locally accumulated negative charge in the transition states. This Mg 2+ is coordinated by reaction products, primer n+1 , and pyrophosphate ( Figure 6C). The nature of this transient divalent cation has been verified by using Mn 2+ instead of Mg 2+ in the reaction buffer. The third metal ion has also been observed in the time-resolved study of the X-Family DNA pol β. 131 In a related study of bacteriophage N4 RNA polymerase (A-Family), the third metal ion is not observed, 132 and its absence could be due to the lack of spatial (2.0 Å instead of 1.5−1.7 Å as in the studies of pol η and β) and temporal resolution, in which a reaction intermediate state was not recorded. In addition to neutralizing the excess negative charge of the reaction intermediates and stabilizing products, the third Mg 2+ may play a role in protonating the pyrophosphate product. 127 The two transient elements, the water and the third metal ion, revealed by time-resolved analyses have escaped notice in conventional studies of either enzyme−substrate or enzyme− product complexes. The third metal ion may be a general feature of DNA synthesis and many reactions catalyzed by the two-metal ion mechanism. The transient nature of the third metal ion may explain the discrepancy between "three metal ions" reported by chemical studies of the reaction process catalyzed by a group I intron and "two metal ions" observed in the crystal structures of ribozyme−substrate and ribozyme− product complexes. 133,134 The time-lapse series of the in crystallo reaction also reveals a delay between substrate and cofactor binding and the actual chemistry, during which a transient water molecule emerges to hydrogen bond with the 3′-OH. Deprotonation of the 3′-OH could be the rate-limiting step, and the details of what leads to its pK a shift and the chemical reaction await future studies.

■ CONCLUDING REMARKS
In the 18 years since yeast Rev1 was first purified and shown to be a nucleotidyl-transferase, Y-Family DNA polymerases have been discovered and characterized. However, many questions are yet to be answered. The most fundamental question remaining is how so many DNA polymerases are coordinated at a replication fork to ensure that DNA synthesis occurs with the highest accuracy and efficiency in the presence of various roadblocks. The molecular details of how DNA polymerases switch when a lesion is encountered remain vague. With regard to the polymerase catalytic region, atomic structures of pol κ bypassing a BPDE adduct are yet to be obtained. While more protein modifications are likely to be found, the importance of the linear arrangement of PIP, RIR, UBM, and UBZ in each polymerase and whether these homologous functional parts can be exchanged between Y-Family members are not known.