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by Don Bleek | November 1, 2012 · 4:25 pm
Shoe Fetish: Rihanna Wearing $1,230 Azzedine Alaïa Mesh Ankle Booties
Last night, pop singer Rihanna was crowned "Queen of West Hollywood" during the city's annual Halloween Carnival. The mayor of West Hollywood presented Riri with the key to the city. Riri was accompanied by her mother Monica. Later that evening, Riri headed over to a Halloween Ball at Greystone Manor and partied it up with Chris Brown and Pais Hilton. For the event, Riri styled in $1,230 Azzedine Alaia Mesh Ankle Booties paired with a $1,545 black Dolce & Gabbana lace panel skirt and a $450 Ribbed Dartford Top by The Row. Rihanna is scheduled to release her new album, entitled Unapologetic on November 19. To support the LP, Riri will embark on her 777 tour which kicks off November 14 and wraps November 20. D
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Tagged as Rihanna, Shoe Fetish: Rihanna Wearing $1230 Azzedine Alaïa Mesh Ankle Booties
LeBron & Savannah, Dwyane & Gabrielle And Chris & Adrienne Showed Off Their Iced Out Championship Rings
Most likely Savannah, Gabrielle and Adrienne weren't with their men shooting in the gym but they have been sitting courtside cheering on LeBron, Dwyane and Chris for the past two seasons. In return, the Miami Heat ballers created duplicate NBA championship rings for the ladies. Peep the pics below:
LeBron's fiancée Savannah
LeBron's and Savannah showing off their rings
Gabby & Dwyane looking happy and deeply in love
Chris & Adrienne Bosh flashing their rings
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Filed under Entertainment
Tagged as LeBron & Savannah Dwyane & Gabrielle And Chris & Adrienne Showed Off Their Iced Out Championship Rings
Jay-Z Brought Back The First Sports Arena To Brooklyn Since 1957
Brooklyn's rapper and Hip-Hop mogul Shawn Corey Carter aka Jay-Z brought back the first sport arena in over 55 years to his stomping grounds. Brooklyn hasn't had a sport arena since Dodgers President Walter O'Malley moved the team to Los Angeles after the 1957 season. O'Malley moved the team to the California coast because the city refused to help subsidize the new stadium.
"When the Dodgers left, it was another punch in the face to the fact that Brooklyn's best days may not be ahead, but may have been behind us. It was depressing," said Brooklyn Borough President Marty Markowitz. Fast-forward to decades later, the $1 billion Barclays Center has received millions in public money and on September 21, 2012, the sport arena was opened to the general public. Nets owner Mikhail Prokhorov, Forest City Ratner Companies chairman and CEO Bruce Ratner and Mayor Bloomberg cut the ribbon in the ceremonial opening. Supporters and Brooklyn natives cheered on as the lights were turned on and the ribbon was cut.
The Barclays Center is a multi-purpose indoor sport arena located on 620 Atlantic Avenue. It sits partly on a platform over the Metropolitan Transportation Authority-owned Vanderbilt Yards. Barclays has all black seats and mental hanging lights with a polished wooden floor design with the Net's black and white logo in the center. It also features an exclusive VIP D'USSE Riser and Jay-Z's 40/40 club which overlooks the court and the streets of Brooklyn. The arena which was designed by the architect firm Ellerbe Becket is named after London-based Barclays PLC. Sources reported that the banking and financial services company has agreed to pay the team between $200-$400 million dollars over the next 20 years for he naming rights.
In 2004, Barclays Center was conceived by Bruce Ratner of real estate's developer Forrest City Ratner, the NY office of Forrest City Enterprises, who also acquired the Nets. The projected date for the arena to open was in 2006 but it was delayed six years because of lawsuits, controversies involving residents, lack of financing and the use of eminent domain. In 2009, it was announced that Russian billionaire Mikhail Prokhorov agreed to become a principal owner of the Nets and a key investor in the arena for a $200 million deal.
Rapper Jay-Z is a minority partial owner of the team and encouraged the team's relocation to Brooklyn from New Jersey. "I feel like Jay-Z did something that no other rapper could do. He's a good business man and role model. He showed people in general that if you work hard you can attain a high level of success, no matter where you come from, said Quazee DeJesus a NY resident.
According to NYC Mayor Michael Bloomberg, "the project has created more than 1,500 jobs." Developer Bruce Ratner said he was glad the arena is finally open after its completion was delayed. The arena also symbolizes the rise of Brooklyn borough in recent years. It will also attract tourists from all over the world.
Having a sports arena in the borough is very impactful because Barclays will also bring a lot of other businesses to Downtown, Brooklyn. This is just the beginning to many of restaurants, shops, boutiques, brokers and apartments. Carlo Scissura, President and CEO of the Brooklyn Chamber of Commerce told the NY Post, "Clearly, this is an incredible opportunity for local and national businesses to explode along Flatbush and Atlantic Avenues." More businesses have to be built around Barclays in order for Brooklyn to become one of NYC's landmarks and business communities. Sources are reporting that Dave & Busters. Panera Bread, TGI Friday's and Starbucks have been shopping for space by the sports arena.
There's nothing better than staying healthy! The house that Jay-Z built features organic and gluten-free fare at its concession stands which includes beer ($9), hotdogs ($5.25) and chocolate bars among other snacks for ($4.75). Barclays is the first arena to feature organic and gluten-free fare. "I think I feel healthier in terms of my stomach and being able to eat and process food faster," Nets players Kris Humphries told Men's Fitness. Jay-Z made history once again!
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Tagged as Barclays Center, Brooklyn Nets, Jay-Z, Jay-Z Brought Back The First Sports Arena To Brooklyn Since 1957
Styling On Them Hoes: Angela Simmons In $895 Giuseppe Zanotti Bow Platform Sandals & Carrying A Chanel Bag


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Having worked with String for many years, Swedish stylist Lotta Agaton created a complete String House for Stockholm Design Week. The incredible display, of which I've seen many beautiful images shared on Instagram, shows the versatility of the iconic shelving units, and was designed to go hand in hand with the new campaign imagery. With impeccable styling by Lotta, these were shot on location at the Villa Frösakull by Jonas Lindvall. Showcasing how the shelves can be used in different areas of the home, I've picked out a few favourites to share with you today.
You all know how much I love our String System, but these have got me thinking about other opportunities at home. The bedroom and wardrobe layout is amazing, but I'm also loving the laundry set up. Ours is calling out for these wall-mounted shelves. The String+ accessories such as the hooks and organisers would also be really useful. For plenty more ideas and inspo, visit String.


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Additional Specialized Areas of Focus
They've had to make a very deep analysis of issues and interpretations under New York law and have done it extremely thoroughly and quickly.
— Chambers USA
We work as a team beautifully.
Our energy clients entrust us with bet-the-company cases worth billions of dollars, but also know we can handle smaller matters efficiently.
Our team has been resolving energy disputes for over 30 years. Our clients span the oil & gas and power/renewables sectors, as well as related industries like mining and shipping. In addition to our extensive construction experience, we handle disputes involving shareholder/JV/partnership agreements, JOAs and concessions, M&A deals, hedging contracts, long-term supply agreements, power purchase agreements, and O&M agreements.
Many of our energy clients' most critical disputes are resolved in arbitration. They turn to us for our market-leading arbitration expertise and insights into the process and key players.
We represent or have represented:
Brazilian company in a tripartite LCIA-administered arbitration under the UNCITRAL Rules arising out of a joint operating agreement. (Brazilian law; Paris seat)
Multinational owners of a power plant in Central America in a $1.2 billion ICC arbitration against a Chinese contractor concerning termination of an EPC contract and financing agreement. (New York law; Singapore seat)
German parent and its US subsidiary in an ICDR arbitration against a Canadian infrastructure company concerning supply of turbines to a major wind farm project in Texas. (New York law; New York seat)
Subsidiary of a European energy company in an ICC arbitration over termination of a Spanish contractor for a BOT hydro dam project in Central America, also involving litigation over security. (New York law; Miami seat)
US energy company in a Singapore-seated SIAC arbitration of post-acquisition claims for breach of warranties/fraud arising from the purchase of a power plant in Pakistan. (English law; Singapore seat)
Multinational engineering company in an ICC arbitration concerning warranties for remediation of a site used for petrochemical activities. (Florida law; New York seat)
Owner of a combined cycle power plant in Guatemala in advising on claims arising out of installation of a defective turbine, including claims against the OEM as well as the installer.
US energy company in an ICSID arbitration against a Latin American state, seeking recovery for breaches of international law arising out of interference in the power generation and distribution markets.
European oil company against a multinational oil company in an ICC arbitration in a dispute arising out of a JOA for a concession in Venezuela. (New York and Venezuelan law; New York seat)
US oil company in an UNCITRAL conciliation against a North African state-owned entity in a dispute concerning contractual renegotiation.
European energy company in an ICDR arbitration over tax allocations in a JV agreement for an LNG project in the Caribbean (and overseeing local litigation strategy). (New York and Trinidad law; New York seat)
African state-owned petroleum company in court proceedings in New York and Washington D.C. regarding the enforcement of a multi-billion dollar arbitration award in US federal court; the arbitration underlying the award involved a complex dispute with JV partners regarding profit-sharing and tax implications.
Brazilian corporation in an ICDR arbitration and parallel US litigation concerning international sales contracts for fuel supply. (New York law; New York seat)
Multinational engineering company in an ICC arbitration arising out of a JV for construction/refurbishment of an oil refinery project in Mexico. (New York seat; New York law)
US power company against an instrumentality of an Indian State Government in an ad hoc arbitration in India. (New York and Indian law; Indian seat)
Middle East oil and gas company in New York litigation against a major US investment bank regarding claims arising out of a complex hedging program.
Multinational company in a SIAC arbitration in Singapore concerning claims for fraud and breach of warranty following the acquisition of a co-generation plant in Pakistan. (English law; Singapore seat)
Multinational energy company on managing and ultimately settling a series of alleged breaches of an M&A agreement for the acquisition of a bundle of energy-related assets arising out of the largest intra-South American energy deal.
European energy company in an international law dispute against a sovereign, with claims brought under the ECT and the applicable BIT regarding the sovereign's regulatory restructuring of its energy sector.
Multinational energy company in reviewing and coordinating multiple commercial arbitrations and litigations affecting power and gas assets throughout Brazil, including settling a long-standing dispute with the Brazilian energy regulator.
James Hosking, Founding Partner
james.hosking@chaffetzlindsey.com
Yasmine Lahlou, Partner
yasmine.lahlou@chaffetzlindsey.com
Related Areas of Focus
Latin America Disputes
Cross-Border Litigation

redpajama_set_name: RedPajamaCommonCrawl


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Perché io credo in Colui che ha fatto il mondo. Tra Fede e Scienza è un saggio scritto da Antonino Zichichi.

Contenuto

Il libro è suddiviso in nove capitoli:

 Sintesi introduttiva
 Che cos'è la scienza
 Distinguere l'uomo da tutte le altre forme di materia vivente
 Evoluzione culturale e biologica
 La scienza e la cultura del nostro tempo
 Dov'è il Paradiso?
 Il pessimismo tecnologico di fronte all'ottimismo scientifico
 Dalla scienza una nuova speranza
 Conclusioni e la grande alleanza tra scienza e fede

Nel secondo capitolo, l'autore ricorda l'affermazione di Galileo Galilei: "Studiando gli oggetti volgari scoprirò le leggi del Creato". Ai tempi di Galilei, gli oggetti volgari erano, per esempio, le pietre, gli spaghi e i legni. Con l'ausilio di tali oggetti scoprì la prima e la seconda legge del moto, mediante il pendolo e il piano inclinato. Attraverso un atto di fede in Colui che ha fatto il mondo, prosegue Zichichi, Galilei diede inizio alla Scienza attuale, basata sul metodo sperimentale.

Appellandosi esclusivamente alla fisica galileiana, Antonino Zichichi ripercorre la vexata quaestio del secolare rapporto tra Scienza e Fede: smentisce che ci sia una contrapposizione e, seppur nel rispetto dell'autonomia delle due aree di azione culturale, plaude all'integrazione della Fede in una civiltà caratterizzata dall'ideale della Scienza razionalista.

Edizioni

Critiche
Il libro è stato oggetto di una stroncatura critica condotta con stile sarcastico dal matematico Piergiorgio Odifreddi, nel suo libro Zichicche II, pubblicato nel 1999.

Note

Voci correlate
Antonino Zichichi

Altri progetti

Saggi di divulgazione scientifica
Saggi di autori italiani

redpajama_set_name: RedPajamaWikipedia


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Q: Difference between arm-none-eabi and arm-linux-gnueabi? What is the difference between arm-none-eabi and arm-linux-gnueabi? I know the difference in how to use them (one for bare metal software, the other one for software meant to be run on linux). But what is the technical background?
I see there is a difference in the ABI which is, as far as I understood, something like an API but on binary level. It ensures interoperability of different applications.
But I don't really understand in which way having or not having an operating system affects my toolchain. The only thing that came to my mind is, that libraries maybe have to be statically linked (do they?) while compiling bare metal software, because there is no os dynamically providing them.
The most pages I found related to this toppic just answered how to use the toolchains but not the technical background. I'm a student of mechatronics and new to embedded systems, so my experience in this field is somewhat limited.

A: Maybe this link to a description will help.
Probably the biggest difference:
"The bare-metal ABI will assume a different C library (newlib for example, or even no C library) to the Linux ABI (which assumes glibc). Therefore, the compiler may make different function calls depending on what it believes is available above and beyond the Standard C library."

redpajama_set_name: RedPajamaStackExchange


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Stay-at-home new order from Sunday in Contra Costa
Contra Costa County, California – Health officials from Contra Costa joined 4 other Bay Area counties to follow much of the state with a new Stay-At-Home order. The County's order takes effect Sunday night.
The new order comes despite the fact that the Bay Area COVID-19 Region is still above the threshold which requires the rules to take effect. On Thursday, Governor Gavin Newsom announced a statewide policy: a region must close down if its ICU bed capacity falls below 15%. The Bay Area Region, consisting of Contra Costa, Monterey, San Francisco, Santa Clara, Alameda, Marin, San Mateo, Solano, Sonoma and Napa Counties, currently has ICU capacity above 15%. However, on Friday, health officials decided not to wait until reaching that point.
"We cannot wait until after we have driven off the cliff to pull the emergency brake," said Santa Clara County Health Officer Dr. Sara Cody.
"We are seeing a surge in COVID-19 cases and hospitalizations here in Contra Costa County and across our region," said Contra Costa County Health Officer Dr. Christopher Farnitano. "The number of patients hospitalized with COVID-19 in our county has doubled in just the past couple of weeks. We are at risk of exceeding our hospital capacity later this month if current trends continue."
"It takes several weeks for new restrictions to slow rising hospitalizations. Waiting until only 15 percent of a region's ICU beds are available is just too late," said San Francisco Health Officer Dr. Tomás Aragon. "Many heavily impacted parts of our region already have less than 15 percent of ICU beds available. The time to act is now."
Stay-at-Home Orders
The new Stay-At-Home order is similar to last spring's lockdown, but some modifications came out of that previous experience. The order bars nonessential gatherings. It requires people to stay at home except for essential activities such as going to the doctor. Grocery shopping or picking up take-out meals is allowed as well. Distanced outdoor exercise such as hiking and biking are permitted. Outdoor religious ceremonies may also continue
Retail stores may continue operating at 20% capacity. Private and charter schools that have re-opened for in-person classes may remain open.
However, playgrounds, indoor recreation facilities, hair salons, bars, casinos, museums, zoos, aquariums, and movie theaters must cease operations. Sports events with live audiences and amusement parks must also close.
Consistent with the State's framework, health officials want to ensure that all sectors have at least 48 hours notice of these closures. Most Bay Area health officers will implement the State's Regional Stay-At-Home Order as of Sunday December 6. In Alameda County, the order takes effect on Monday, December 7. Marin County's order take effect Tuesday, December 8. The new restrictions will remain in place until January 4, 2021.
More in Contra Costa County
Walnut Creek, California – The Ygnacio Valley Library in Walnut Creek has been used as a...
Public's assistance sought in locating armed murder suspect
Antioch, California – Police in Antioch are appealing to the public for assistance in the search...
Burglars broke into Orinda homes Thursday
Orinda, California – Things have been a little busier than normal in Orinda lately, with neighbors...
Man arrested after trying to sexually assault a girl because he despised black people
Walnut Creek, California – After being arrested on allegations of attempting to sexually attack a young...
California State Prison inmate considered main suspect in the death of Contra Costa County cellmate
Contra Costa County, California – During the weekend, a prisoner from Contra Costa County serving a...
Super Holiday Marketplace this weekend – Holiday gifts galore at free
Businesses take advantage of final days before Sunday's lockdown


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Home > Banking & Finance
World's central bankers feel the heat as populists seek to dictate policy direction
Nations like the US and Turkey have exerted pressure on their central banks to loosen policy, alarming technocrats
Earlier this year, Italy's ruling parties floated the idea of getting the European Central Bank to forgive some of the country's huge debt pile or offer a guarantee on it - a suggestion that was rebuffed by ECB president Mario Draghi.
PHOTO: BLOOMBERG
Nusa Dua, Bali
CENTRAL bankers from the United States to Europe are coming under pressure from their governments to loosen their purse strings again as a decade of accelerating economic growth and booming markets fuelled by cheap cash comes to an end.
For 10 years since the global financial crash of 2008, the Federal Reserve and other central banks across the world have engineered a global upswing through ultra-low interest rates and massive money-printing programmes.
As inflation recovers, rate-setters are now tightening their policy, leaving indebted governments, entrepreneurs and households feeling the pinch from higher borrowing costs and, in fragile emerging economies, sliding currencies.
What started as an issue for countries with sizeable US dollar debt such as Argentina and Turkey is now reaching the world's richest and supposedly most advanced economies in the United States and the eurozone.
SEE ALSO: Japan sets up working group on impact of Facebook's Libra ahead of G7
US President Donald Trump launched a scathing attack on the Federal Reserve last week for being "too aggressive" in raising rates and labelled the institution "crazy", "loco", "ridiculous" and "too cute".
Earlier this year, Italy's ruling parties floated the idea of getting the European Central Bank (ECB) to forgive some of the country's huge debt pile or offer a guarantee on it; the central banks of Turkey and South Africa have also been blamed by their governments for being too tight with their policy.
The clash threatens to tarnish the golden rule of developed financial systems for the past three decades: keep the money-printing presses away from elected politicians if you want to keep inflation in check. This risk, part of a global wave of populism and aversion towards technocrats, worried policymakers at the International Monetary Fund's annual meetings last week.
"They're asking to change rates, (debt) cancellation, they're asking them to do other things, buy bonds," ECB president Mario Draghi told a press conference at the IMF meeting on the Indonesian resort island of Bali. "This is one of the risks in the geopolitical sphere."
Barry Eichengreen, a professor at Berkeley, said governments had more to lose than gain by discrediting their central banks. "Seriously infringing on the independence of the central bank would elicit a negative market reaction," he said.
So far, monetary guardians have stood their ground. Eurozone central bank sources at the IMF meeting told Reuters Italy won't receive any help from the ECB unless it secures a bailout from the European Union, which comes with stringent conditions in terms of budget tightening and economic reforms.
The Fed's chair, Jerome Powell, did not react to Mr Trump's comments but he bagged a resounding endorsement from IMF managing director Christine Lagarde. Another central banker attending the Bali meeting said he was not giving Mr Trump's comments too much weight. "I'd take him seriously if he removed Powell but I don't think he'd do that," the official said.
And Lesetja Kganyago, governor of the South African Reserve Bank, said at the Bali summit rate-setters needed to be "given the space to act independently without political interference".
Even in countries where central bank independence is weaker or absent, monetary guardians are putting up a fight. Turkey's central bank last month raised its benchmark rate by a hefty 625 basis points to boost the lira despite harsh criticism from President Tayyip Erdogan.
But Richard Portes, a professor at the London Business School, said the issue wasn't likely to go away until central bankers became more accountable and transparent.
"Monetary policy has political consequence even though central banks are run by technocrats," he said. REUTERS
China's yuan internationalisation should be market-driven, will take time-regulator
Australian, New Zealand dollars hold firm, domestic stimulus long priced in
Investment firm Varde Partners appoints Singapore-based co-CEO
Nomura to pay US$25m over mortgage bond traders' lies


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Health Benefits of Colloidal Silver
Colloidal silver is a broad spectrum anti-microbal and immune system enhancing supplement. It provides the human body with silver, a necessary trace mineral. It supports health, not only as an alternative to drugs, but as a safe disinfectant.
It has a long history of usage as a preservative and until 1938, it was in common usage. Despite attempts to discredit colloidal silver, it is used as a nutritional supplement by millions of people on the North American continent and it has been the subject of numerous scientific studies around the world.
History and Basic Information
Antibiotic and Fungicide
Even Drug-Resistent Mutant Strains are Killed by Colloidal Silver
Colloidal Silver Eliminates Parasites
Colloidal Silver is a Powerful Antiviral
Enhanced Immune System Function
Silver is a naturally occurring trace mineral, however, because of modern farming methods that have depleted the soil, silver is almost non-existent in the modern American food supply. Supplementing with colloidal silver provides this trace mineral, necessary to the protection and functioning of healthy cells.
According to John W. Hill in his book, "Colloidal Silver: Medical Uses, Toxicology and Manufacture," the mineral silver has a long history of use. He says, "The Greeks, Romans, Egyptians, and others used silver vessels for water and other liquids to keep them fresh." The medicinal properties of medicine were recognized by the alchemists, who were constantly criticized by the doctors of their day for their consumption of minerals.
But, by the late 19th century, allopathic physicians began using silver compounds in their medical practice. Although, it must be noted that these were not always safe substances because when silver binds with other types of molecular particles, it creates completely different compound substances.
Pure, unadulterated colloidal silver nanoparticles have a positive electric charge. When ingested or inhaled through a nebulizer, silver nanoparticles enter the bloodstream and, thus, the tissues and cells of the body. Silver particles protect cells from the invasion of viruses. They attack bacteria, fungi and other single-celled organisms, by magnetizing to the outside surface and interfering with their respiration.
Colloidal silver is a proven killer of bacteria. It is a far more efficient antibiotic than any allopathic pharmaceutical, leaving no anti-biotic resistant mutations behind. In a 1999 study conducted by independent researchers at Brigham Young University, once published on the Internet and referenced in an article by Lois M. Collins in Deseret News, May 16, 2000, showed that colloidal silver was effective in killing 650 bacteria in less than five minutes, at most, in concentrations of five to six parts per million (ppm). This study is one of the most frequently cited because of the broad range of bacteria that was included in the research. No further research was possible by the department as it was unable to secure a government grant, in spite of the results of this study, which were no doubt a startling revelation to the world of allopathic medicine.
This was not new information to colloidal silver users, however, who have found that this mineral has corrected a wide variety of bacteria-related illnesses as well as number of diseases defined as "syndromes" by allopathic doctors who remain mystified by the multiplicity of causes and symptoms involved. Many of these causes and symptoms are addressed by the scientifically known, broad spectrum, antimicrobal and restorative properties of colloidal silver.
Staphylococcus aureus, salmonella, enterobacter, bacterial pneumoniae and streptococcus are among the most common bacteria that colloidal silver eliminates. It has the power to kill emerging strains of bacteria, which are iatrogenic in origin, often the result of the inefficiency of pharmaceutical bacteria and the tendency of bacteria to mutate and become resistant to these drugs.
An example of this is the effect that colloidal silver has on iatrogenic Community Acquired Methicillin resistant Staphylococcus aureus (CA MRSA). This mutant strain of Staph is a deadly threat to patients in dirty hospitals. In an October 2006 study by Souza, Mehta, Leavitt of the Department of Microbiology/Molecular Biology of Brigham Young University, published in Current Science, Vol. 91, No. 7, October 10, 2006, it was demonstrated that multiple-drug resistant bacterial strains, like MRSA, are destroyed by colloidal silver nanoparticles.
In a similar fashion, colloidal silver interferes with the respiration of a variety of fungi, yeasts and molds. It has both internal and topical applications and can be sprayed inside and outside the home to safely eliminate these pathogens.
Colloidal silver is also an anti-parasitic. It works by attacking bacteria when they are in their egg-producing stages. Colloidal silver nanoparticles bind with the cell walls of single-cell organisms and inhibits their respiration.
Dr. Hulda Clark, Ph.D., N.D., the author of the book, "The Cure for All Diseases," cites parasitic infections, little recognized or understood by allopathic physicians, as the cause for many diseases in humans. When parasitic infections are eliminated, many human diseases and syndromes disappear.
It has applications for farming, gardening, household disinfection, pet and livestock disinfection and human health. It can safely be used both topically and internally. Colloidal silver is especially useful for preventing the cross-contamination of humans with animal parasitic infections.
Colloidal silver kills a broad spectrum of viruses. It is effective against even the most feared and deadly viruses, which are unaffected by allopathic antivirals or other treatments.
Most notably, colloidal silver has been shown, both scientifically and anecdotally, to kill HIV, AIDS, strains of Hepatitis and even emerging strains of flu viruses. Otherwise hopeless cases have been restored to health. Sometimes this is done solely through the use of ingested or inhaled colloidal silver. But, some therapies in countries outside the U.S. have successfully employed an injected form of pure silver nanoparticles.
Because colloidal silver provides the body with a necessary trace mineral and because it possesses the aforementioned antimicrobal properties, it enhances the functioning of the immune system and has a positive effect on overall human health. Colloidal silver users unanimously report an improvement in overall health and a resistance to common viruses.
Colloidal silver has been shown to be effective, at least, anecdotally against disease that is associated with immune system malfunction. This includes skin disorders, chronic fatigue syndrome, lupus, epstein-barr, and a variety of other conditions. The primary reason that most people take colloidal silver is to elevate the functioning of the immune system and to make their bodies more resistant to disease.
Properly manufactured, pure colloidal silver is generally non-toxic. The only exception is those sensitive individuals who are allergic to silver and cannot wear silver jewelry. Colloidal silver nanoparticles do not accumulate in the tissues, but are passed through the body. A standard dosage for the average human being is 1 to 2 tablespoons per day.
http://www.ehow.com/about_5065463_health-benefits-colloidal-silver.html
Colloidal Silver Spray, 250ppm, 2 oz.
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Polycystic Ovary Disease (PCOD)
Dear Al & Tammy, During March of this year my doctor informed me I had what she believed to be Poly...
By Christine S. Koch


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Home Team News New Events Our Members Become A Member
HomeTeamNewsNew EventsOur MembersBecome A Member
Trowbridge Town Hall Market Street BA14 8EQ United Kingdom (map)
What? Walk 10k raise £130
Where? Starting and finishing at Trowbridge Town Hall
When? Sunday 24th June registration from 1pm walk sets off 1.30pm
Why? Our town hall is under threat we have to save this iconic building. It has been a part of our town for 130 years.
Our town hall is under threat of closure so here at the newly invigorated chamber we are doing our bit to help raise funds for not only an historic building and an important resource for the town but an icon for Trowbridge that has played a part in many peoples lives and that of the chamber.
The Town Hall has until the end of June 2018 to raise a minimum of £35000 or close down forever at the end of July.
On 24th June 2018 at 1.30pm Trowbridge business owners/art groups will be getting together to do a 10k walk around the town collecting donations along the way.
Our challenge is to find 130 business people to raise a minimum of £130 each, do the 10k and take part in our arty Instagram challenge along the way.
We aim to raise at least £16900.00 through our efforts.
We don't want to see the closure of this brilliant town centre facility and wish to do our bit in any way we can. Please support your Town Centre, make a donation and come to support us on the 24th June.
"The earth without art is just eh" Demitri Martin.
To register your interest and receive your welcome pack click here
Source:: https://localgiving.org/fundraising/130challenge/
One Minute Meet Up
Ginstagram


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6 Epidemics, Generalist, Researcher, Scientist.
But things were looking grim: we had six outbreaks, including three outbreaks at once from a nasty triangle chain reaction, and we had lots of three-cube cities on the board. If we'd drawn another epidemic, we would have been in a very rough spot.


This morning we got going early to be on our bird tour at 6:30, before breakfast but not as early as it would seem here in the US. Our guide, Carlos, actually liked children and was good with ours. He asked us each what we most wanted to see. I really wanted to see a toucan, and Genevieve really wanted to see an anteater, and Charlotte and Mark didn't really have an answer. Right away he spotted a red billed toucanet in his scope. We walked on and saw the monkeys all again and other birds but nothing really compelling. I mentioned that I had read about the pond in the tropical garden but had been unable to find it. He said "do you guys like frogs?" to which we of course answered yes. So we headed the tropical garden pond and what should we see but hundreds upon hundreds of green leaf frogs! (see movie here). Apparently they mate at the pond after the full moon, which it was that day. It was one of the coolest things I've ever seen. Next we had breakfast and arranged to go to the beach later in the day. We asked for a car to drive us both there and back although most people hike there and then get a ride back, because of Charlotte's aversion to walking. We hung out in the cabina for a while, and went to lunch where we talked to a couple of honeymooners staying in the cabina next to ours. I mentioned that I still hadn't seen a toucan and they said "really, there was one in our tree earlier today". The guy told me that they made "a really obnoxious chirping" that was hard to miss. After lunch we met our ride to the beach. We got the same driver we had on the trip up, which was nice. When we arrived at the beach, who should we see but the woman from lunch who had arranged with Lapa Rios to have us on a tour the next day at 3:00. Too nice! We chatted with them for a while and then followed the noise to the scarlett macaws. There were a least a dozen of them in a tree right on the edge of the beach! We watched them for a while, then went back to play in the waves and with the hermit crabs, and then back to see the birds again. While we were there a band of squirrel (titi) monkeys came to the tree, including this mommy and her baby. So cute! The car came to pick us up right on schedule, and we headed back to the pool at Bosque. While we were there, I heard a very distinctive noise that I figured had to be a toucan, so I followed the chirping and sure enough it was a chestnut billed toucan up in the tree right next to our cabina. Too cool. We cleaned up in our outdoor bath and shower and went to the bar and then to dinner and bed.


(34) - job's found after searching.
GSL Education are currently seeking to appoint an outstanding Computer Science Teacher to join a friendly and dynamic Ofsted rated 'Good' school.
The role is at a mixed secondary school based in Tower Hamlets, London. The school seeks to appoint an inspiring, ambitious and highly motivated Computer Science Teacher with a real passion for learning and teaching, to join a successful Faculty from September 2019 (earlier for NQT).
- Be a qualified Computer Science teacher (ideally with PGCE, GTP, B'ed, Cert Ed, BA with QTS).
- Hold good honours degree or equivalent in Computer Science.
- Demonstrate a clear and well-thought-out understanding of current educational issues, theory and practice.
- Have a detailed knowledge of the relevant aspects of the students' National Curriculum and other statutory requirements.
We welcome applications from all Computer Science Teachers who feel that they can add value to this school and who can prosper in a highly effective, fast-paced environment. Candidates wishing to apply are requested to forward a copy of their CV to our Education Consultant, Ryan Jordan, as soon as possible.
GSL Education are currently looking for a qualified English Teacher to join a high achieving secondary school graded 'OUTSTANDING' by OFSTED. This post will be a temporary post, with an immediate start until the end of Academic year.
The role is at a successful 11-19 mixed secondary school, located in Tower Hamlets. The school is a thriving, diverse and successful comprehensive high school, which is committed to high standards.
The school is looking to appoint an inspirational English Teacher who shares their belief in the highest standards of academic attainment in a comprehensive setting. The successful candidate should be an innovative and dynamic English Teacher who is able to teach at KS3 to KS4.
- Demonstrate recent experience as an English Teacher or trainee English Teacher.
- Hold good honours degree or equivalent in English.
We welcome applications from all the interested candidates, who feel that they can add value to this school and can prosper in a highly effective, fast-paced environment. Candidates wishing to apply are requested to forward a copy of their CV to our Education Consultant, Sonia Zamir, as soon as possible.
Are you an outstanding and inspirational teacher?
Are you committed to ensuring the best possible outcomes for children in a stimulating environment?
Do you want to join an effective and friendly team?
GSL Education are looking for a dynamic, motivated and experienced KS 1 & 2 teacher. Successful candidates are likely to have a good working knowledge of Special Education Needs and will be a qualified teacher. The candidate needs to have solid understanding of the national curriculum. Moreover, needs to be a strong teacher with good behaviour management.
The school is based in East London & it's an independent school. The school follows the statutory EYFS guidance, and the KS1 and KS2 curriculum is broadly based upon the National Curriculum; however, as an independent school they retain the right to devise their own programmes of study where it is deemed appropriate, to enrich the pupils' learning. Teachers always reflect upon their practice to ensure the highest standards of teaching and learning are maintained.
Have a passion for teaching and learning across the whole curriculum with a particular strength in KS1 & KS2.
Be totally committed to inclusion and equality.
Have the capacity to work collaboratively with all stakeholders to drive standards forward whilst making school a happy place for children to learn.
If you are passionate about quality teaching and interested in experiencing the flexibility of the supply world, please send your updated CV to our Senior Primary Education Consultant, Hinal Patel, as soon as possible.
GSL Education are currently looking for a qualified English Teacher to join a high achieving secondary school graded 'OUTSTANDING' by OFSTED. This will be a maternity cover until December, with immediate start.
The role is at a successful 11-19 mixed school, located in Tower Hamlets. They are a complete school that prioritizes on every aspect of life. The school have achieved excellent results both at GCSE and at A-Level.
The school is looking for someone who can plan and teach English lessons to students of KS3 & KS4, that follow the agreed departmental schemes of work and teaching guidelines.
- Be able to teach English up to KS4.
We welcome applications from all interested English Teachers, who feel that they can add value to this school and can prosper in a highly effective, fast-paced environment. Candidates wishing to apply are requested to forward a copy of their CV to our Education Consultant, Brittany, as soon as possible.
GSL Education are currently seeking to appoint an outstanding Business & Economics Teacher to join a friendly and dynamic Ofsted rated 'Outstandiing' school.
The role is at a mixed secondary school based in Tower Hamlets, East London. This is a permanent role, starting from September 2018. The school encourages candidates to apply at the earliest possible stage, as it reserves the right to interview and appoint at any time during the process, in some cases in advance of any formal closing date.
The BIS faculty teaches Economics, Business, ICT and the Social Sciences. The school are seeking to appoint an exceptional teacher of Economics & Business to join its journey to be an exceptional school.
As such, The school is looking for someone who can plan and teach Business & Economics lessons to classes in years 7 to 11 and will be committed to delivering consistently good lessons. The successful candidate will contribute to the Department's medium and long-term planning to improve pupil outcomes.
The school would welcome applications from both NQTs and more experienced members of staff.
- Be a qualified Business & Economics teacher (ideally with PGCE, GTP, B'ed, Cert Ed, BA with QTS).
- Hold good honours degree or equivalent in Business & Economics.
GSL Education are currently looking to appoint an exceptional Textiles Teacher for a maternity cover at one of our secondary schools in East London.
This is an excellent opportunity for someone wishing to further develop their career and to work with a passionate department who aim to provide a positive learning experience for their students.
The school is based in the borough of Tower Hamlets and is easy to get to with multiple public transport links. It is a coeducational secondary school and sixth form with great facilities and teaching spaces. It has been judged to be "Good" by OFSTED.
The students at this school enjoy a well ordered, safe and respectful environment and are able to achieve excellent results because of the school's high standards of teaching and support.
• Be an experienced Textiles Teacher with great subject knowledge.
• Have experience of teaching Textiles in a previous role.
GSL Education are currently seeking to appoint an outstanding Second in Charge Science teacher to join a friendly and dynamic Ofsted rated 'Outstanding' school.
The role is at an all-girls secondary school based in Tower Hamlets, London. The school is looking for someone who can start from September 2018. This is a permanent role.
The successful candidate should be positive, creative and have a genuine, engaging enthusiasm for the teaching and learning of Science. The Candidate should also be able to achieve high levels of student engagement, challenge and progress.
The successful candidate should be able to contribute in raising the standards of student achievement in by teaching a timetable of lessons and supporting the subject leader in the achievement of the whole school and department goals as stated in the Science department's development plan.
- Demonstrate recent experience as a Science teacher with some leadership responsibilities.
GSL Education are currently seeking to appoint outstanding SEN TAs to work on a daily basis at a friendly and dynamic Ofsted rated 'Outstanding' school.
This is at a lovely all-girls secondary school located in Tower Hamlets and would be day-to-day supply work depending on what the school requires. They are looking for bright individuals who are enthusiastic, empathetic and show initiative. Experience working with SEN students before would be a bonus.
This School strives to ensure that all aims and policies of the school include and apply to pupils with Special Educational Needs and disabilities. This process of inclusion relies on the involvements of Specialist School staff, as well as Teaching Assistants together with the SEN teaching team, to be effective.
- Understanding of how to support the inclusion and progress of pupil with emotional and behavioural needs.
- Ability to build relationships with students, other members of teaching staff and senior management.
- Strong communication and behaviour management skills, as well as possessing the ability to remain calm under pressure.
GSL Education is an ethical, independent recruitment agency serving Teachers and Teaching Assistants across Greater London. Our advertised teaching roles relate to real vacancies that exist at our client Schools.
We welcome applications from all teaching assistant who feel that they can add value to this school and whom can prosper in a highly effective, fast-paced environment. Candidates wishing to apply are requested to forward a copy of their CV to our Education Consultant, Brittany, as soon as possible.
GSL Education are currently seeking to appoint an outstanding Biology teacher to join a friendly and dynamic Ofsted rated 'Outstanding' school.
The Science Faculty is one of the highest performing curriculum areas in the school. The successful candidate will be an outstanding teacher of GCSE and A Level Biology. The school is looking for someone who will be able to demonstrate an unwavering commitment to raising student progress and standards.
- Be a qualified Biology teacher (ideally with PGCE, GTP, B'ed, Cert Ed, BA with QTS).
- Demonstrate recent experience as a Biology teacher.
- Hold good honours degree or equivalent in Biology.
GSL Education are currently seeking to appoint an outstanding Music Teacher to join a friendly and dynamic Ofsted rated 'Good' school.
The role is at a mixed secondary school based in the London borough of Tower Hamlets. The school is looking for someone who can start immediately on a temporary basis until the end of the academic year.
As such, the school is looking for a well-qualified and highly motivated individual who is an enthusiastic and dynamic professional and committed to delivering successful Music lessons for KS3. This is a part-time post for 3 days a week (i.e.- Tuesdays, Wednesdays and Thursdays). Candidate must be passionate about their subject and able to engage and inspire students through the study of Music. The successful candidate should demonstrate good subject curriculum knowledge (UK Curriculum) and maintain students interest in the subject.
GSL Education are urgently seeking a confident, outstanding Economics Graduate for a mentoring position at a vibrant secondary school located in the London Borough of Tower Hamlets.
Our client is a large, vibrant and thriving secondary school in the centre of London. With approximately 1000 students on roll and a further 210 at KS5, staff at our client school work alongside outstanding colleagues to achieve outstanding student outcomes in all key stages. There are over 60 permanent teaching staff and more than 100 support/administrative staff working here.
The role is at a mixed secondary school in the London borough of Tower Hamlets. The school is looking for a part-time Music teacher who can start immediately and this is a temporary role.
Music is a popular subject at this school. At Key Stage 3, students are taught a wide range of topics from notation to world music. There is an extensive instrumental music programme in this school that all students are encouraged to join.
- Be able to teach Music at KS3 & KS4.
The role is at a large, vibrant and thriving secondary school in the centre of London. The school is looking for someone who can start immediately.
The Maths Faculty is one of the highest performing curriculum areas in the school (achieving an overall GCSE P8 score of +0.65). Building upon the success of previous years the school is now aiming to achieve a GCSE P8 score of +1 and an ALPS score of 1 by 2020 and the Maths teacher will play a pivotal role in this journey to achieving excellence.
As such, The school is looking for someone who can plan and teach Maths lessons at GCSE and A Level. The successful candidate will be able to demonstrate an unwavering commitment to raising student progress and standards.
- Hold good honours degree or equivalent in Maths.
GSL Education are currently seeking to appoint an outstanding Geography teacher to join a friendly, dynamic Ofsted rated 'Good' school.
The role is at an all girl's secondary school based in Tower Hamlets. The school is looking for someone who can start from September 2018 for a permanent position.
The school is proud of its vibrant and diverse community and culture, and it doesn't take long to realise that staff are friendly, skilled and highly professional and the students are talented and enthusiastic to learn, as they grow to become tomorrow's women.
This is an excellent opportunity for an outstanding Geography teacher to join this dynamic department working with a well-developed and cohesive team that achieves good results across all key stages. The school is welcoming applications from newly qualified and experienced Geography specialists.
As such, the school is looking to appoint a well-qualified, enthusiastic and dynamic teacher of Geography. Applicants must be able to teach Geography up to KS5, and they will be expected to demonstrate a track record of excellent teaching and purposeful learning.
GSL Education are currently seeking to appoint an outstanding History teacher to join a friendly, dynamic Ofsted rated 'Outstanding' school.
The role is at a mixed secondary school based in Tower Hamlets. The school is looking for someone who can start Immediately. This is a temporary position for the remainder of the academic year in the first instance.
The school provides a range of opportunities to its teachers and support staff to develop their careers. It offers a nurturing and caring environment enabling the teachers to flourish and thrive in a setting that promotes leadership, innovation and creativity.
As such, the school wish to appoint a candidate who is an enthusiastic and inspiring History teacher and who can teach history to KS4 and KS5 students.
- Be a qualified teacher of History (ideally PGCE, GTP, B'ed, Cert Ed, BA with QTS).
- Be able to teach History up to KS5.
GSL Education are currently looking to appoint an exceptional Sociology/Citizenship to join from 16th April at one of our secondary schools in East London. The successful applicant will have to teach Sociology to A Level, Citizenship GCSE and Social Sciences to GCSE.
Be an experienced Sociology and Citizenship Teacher with great subject knowledge.
Be a qualified teacher with QTS or QTLS.
GSL Education are currently looking for a qualified MFL teacher to undertake a term role starting after Easter at one of our secondary schools in East London. The person filling the role will have to teach KS3+4 French and KS3 Spanish.
GSL Education are seeking a Technician with strong Design and Technology knowledge to provide technical support in Resistant Materials. You will support students and teachers in practical lessons, prepare materials, work in conjunction with teaching staff to develop new projects and maintain the school workshop and equipment.
Our client is a vibrant, multi-cultural school in the London Borough of Tower Hamlets with a roll of 880 students and is proud of, and value, the diversity of languages, cultures and experiences that the students bring with them. The school has excellent partnerships with businesses, sports and arts organisations. These partnerships are wide ranging and benefit their students both academically and socially. They have a clear vision for the future, driving forward continued improvements and providing an excellent environment for learning.
GSL Education are seeking a dynamic and motivated Facilities Manager to undertake a role from ASAP at one of our secondary schools in East London.
The role is at a mixed secondary school of over 1000 students on roll and it also has a collaborative sixth-form. It is situated in the heart of the historic east end of London and is less than a mile away from the City of London. It is a large, vibrant and thriving school in the centre of London and has been judged to be 'Outstanding' by OFSTED.
This is an exciting role ideal for someone hands-on, eager to learn and develop and ready to take on a management position.
To take responsibility for all aspects of the school site and grounds ensuring that they are maintained in a safe, clean and secure condition.
To lead, inspire and manage assigned staff to ensure the smooth and efficient running of a safe, secure, attractive and clean learning environment in which staff and students can achieve their full potential.
To manage out-sourced services to ensure agreed school priorities are effected.
To be innovative in promoting the school's site and facilities for both income generation and marketing purposes.
To be the principal Health and Safety Officer.
To take responsibility for assigned budgets and ensuring Financial Procedures are adhered to.
An overall knowledge of systems and processes necessary to ensure that buildings and commercial kitchens function effectively.
Good working knowledge of relevant health and safety regulations.
Experience in a similar role and of managing staff.
Honesty, reliability, flexibility and integrity.
Candidates wishing to apply are requested to contact Taryn at GSL Education on 02077392211 as soon as possible.
GSL Education are currently seeking an experienced Headteacher's PA to undertake an on-going post, Temp to Perm to start ASAP at one of our schools in East London.
The role is at a mixed all-through school located in the London Borough of Tower Hamlets, where employees are valued and developed.
The School moved into an award winning new building with outstanding facilities in January 2011, also the area surrounding the school has recently undergone a major improvement. The school has been judged to be "Outstanding" in every category by OFSTED.
An experienced Head Teachers Personal Assistant who will provide a confidential, high quality personal service to the Head Teacher.
An excellent communicator, who is highly proficient in using Microsoft Office Word, Excel and Outlook.
An enhanced DBS certificate that is registered on the Update Service, or be willing to complete one.
For more information on the role, school and how to apply, please contact Taryn at GSL education on 02077392211.
GSL Education are currently looking to appoint an exceptional Maths Teacher to join one of our secondary schools in East London.
• Be an experienced Maths Teacher with great subject knowledge.
• Have experience of teaching Maths in a previous role.
• Must have GCSE Maths.
We are currently looking to appoint an exceptional Science Teacher to join one of our secondary schools in East London. This role is a maternity cover which will start after Easter 2018. This is an excellent opportunity for someone wishing to further develop their career in a successful school.
The school is an over-subscribed, 11-18 all boys Community \school and it is situated in the heart of the historic East End of London. Its students' progress exceptionally well and standards of achievement at the school have steadily improved over time.
The school is a vibrant and exciting place to teach, with staff who are extremely supportive of each other. In 2011, Ofsted judged the school as "Outstanding".
We are looking for a Science Teacher who is dynamic in the classroom with the ability to differentiate the learning material to a range of ability levels.
- Be a qualified Science teacher (ideally with PGCE, GTP, B'ed, Cert Ed, BA with QTS) specializing in Chemistry or Physics.
We welcome applications from all teachers who feel that they can add value to this school and whom can prosper in a highly effective, fast-paced environment. Candidates wishing to apply are requested to contact Taryn at GSL Education on 02077392211 as soon as possible.
The role is at an 11-18 secondary academy located in Tower Hamlets, East London. It serves an urban, inner city and multi-ethnic intake with a very high percentage of pupil premium students.
build on the academy's many outstanding strengths to create a system-wide role in school improvement and cutting edge professional practice.
have oversight of one of the academies in the Trust.
This is a unique opportunity for a talented leader with vision, drive and ambition, and the desire to make a real difference to our pupils and community within a supportive MAT leadership group.
If you are interested in applying for the role, please send your CV to Ryan at ryan@gsleducation.com.
We are an equal opportunities employer and we are committed to safeguarding and promoting the welfare of children.
GSL Education are seeking an enthusiastic, qualified Teacher of Religious Education for a long term role at one of our outstanding secondary schools in East London.
This is a great opportunity for a strong Religious Education teacher to join a passionate department who aim to provide a positive learning experience for their students. This is a role starting from the 5th of February 2018.
The school is based in the borough of Tower Hamlets and is easy to get to with multiple public transport links. The school is a large, oversubscribed All Girls secondary school and has great facilities and teaching spaces. It has been recently graded as Good by OFSTED in its most recent inspection.
The individual must have the ability to motivate students and achieve excellent results and have the capacity to work collaboratively as part of a successful team.
• Be an experienced RE teacher with great subject knowledge.
A 'Good' with 'Outstanding Features' school is looking for a Teaching Assistant to start in January, full time until the end of the year.
GSL Education is urgently seeking Cover Supervisors for schools in the London Borough of Tower Hamlets. We run a very busy 'day to day supply desk'- as well as getting long-term positions through, and require strong, reliable and experienced Cover Supervisors immediately. The role of a Cover Supervisor involves: -Sole responsibility for supervising classes and issuing work that has been set by the absent class teacher; -Managing the behaviour of pupils whilst they are undertaking this work to ensure a constructive environment; -Responding to any questions from pupils about process and procedures; -Dealing with any immediate problems according to the school's policies and procedures; -Collecting any completed work after the lesson and returning it to the appropriate teacher; -Reporting back as appropriate using the school's agreed referral procedures on the behaviour of pupils during the class, and any issues arising. There are no qualifications necessary to work as a Cover Supervisor but you should feel confident to manage a class of students and to perform the above duties. If you are interested in working as a Cover Supervisor in the Tower Hamlets area please email your CV to Taryn ASAP. We look forward to hearing from you!


text:
© PRONOB GHOSH
Kolkata Night
Boat Market
Bangladesh is a riverine country, so boat is one of the major mode of transportation in the rural areas specially during monsoon. During the monsoon season the rivers, ponds and other water bodies are filled with water, and at that moment the popular & the cheapest source of communication is a boat. Sometimes boat transportation also becomes a small business for some people during the heavy rainy season. For this reason a lot of boat market sits all over the country, and one of them is in kaikertek, situated in Mograpara union near Narayanganj district. This market is almost 200 years old. After a good conversation along with some tea with the boat businessmen, they told me this market always sits during the monsoon season. Every Sunday from 9 in the morning the boat dealers gather and organize their boats, then from 10 the customer starts gathering. People from all over Narayanganj gather to buy boats according to their requirement. The price is usually determined by the quality of the wood and everyday an average of 80-90 boats are sold. At present the boat market is almost in a stage of extinction and the sellers are finding very hard to earn any profit from this business, but some people have kept it alive due to family tradition.
Veer Yatra
Veer Mhaskoba Yatra is held in Magh month. It is the annual fair and festival at a sacred place (Veer Mhaskoba temple or mandir) at Vir village in Pune District of Maharashtra. This annual jatra attract scores of devotees. Veer Mhaskoba Yatra 2019 date is February 19. The temple is located around 26 km from Saswad and 32 km from Jejuri.
Veer Mhaskoba is the manifestation of the Kal Bhairava form of Shiva.
The shrine is decorated with lamps for the occasion.
Special pujas and rituals are held. Processions are another highlight.
Veer Mhaskoba Yatra is held annually on Magh Shukla Paksha Chaturdasi tithi or the fourteenth day during the waxing phase of moon in Magh month as per traditional Hindu lunar calendar followed in Maharashtra.
Kumbh
The Procession of Weapons
Hola Mohalla is one of the biggest festival celebrated by the Sikh community. It's a one day Sikh festival which most often falls in March and takes place on the second day of the lunar month of Chett, a day after the Hindu spring festival Holi but sometimes coincides with Holi. Hola Mohalla is a big festive event for Sikhs around the world. The words 'Hola Mohalla' would thus stand for 'the charge of an army. The festival was founded by Guru Gobind Singh, the tenth Sikh Guru. The Guru was in the midst of fighting both Aurangzeb of the Mughal Empire and the Hill Rajputs, and had recently established the Khalsa Panth. On 7 March 1701, Guru Gobind Singh started a new tradition by overseeing a day of mock battles and poetry contests at Lohgarh Fort. The tradition has since spread from the town of Anandpur Sahib to nearby Kiratpur Sahib and the foothills of the Shivaliks, and to other Gurdwaras around the world.Thousands of people gathers around the sunny spring of Haryana and Punjab and the most interesting thing that amazed me was their entire visual. People dressed for war, ready with guns, swords & shields just to pay tribute to the festival. Starting from adult to children everyone is dressed as a fighter. Alongside the festival there is a fair which showcases all the traditional equipments for the festival & there is a huge gathering there. At that moment I decided I want to do a portrait series of the festival. I felt every character has a different look and a story to tell.
White Priority
Bluecity
Santal village
Santals had been living in the north of this country for a very long time. The colours & patterns they withhold among them & the culture they have are very different & very bright compared to the lot of other ethnic people residing in this country. The most vibrant part of their culture is their unique way of colouring their houses. The patterns they create are uniquely beautiful & eye catching. There are two types of Santals, one who are Hindu & one who are Catholic Christians. During the festival of Diwali the Hindu Santals paint their houses & during christmas the Catholic Santals paint their respectively. They are very creative & they arrange their house in a very proper way which had been forever going on for generations. Some of the noted festivals they celebrate are Sohrai, Bandana, Christmas & the most colourful one is the wedding. The whole colouring of the house revolves around these festivals. Santals mostly resides in the north of Bangladesh such as Chapaynabaganj, gaibandha, Rajshahi, dinajpur. Being one of the ethnic group of this country they are indeed a very colourful bunch of people & for that we as a whole nation should preserve this very unique cultures that has been breathing for ages. Some recent issues caused a lot of damages to them as they are one of the group which is slowly fading away & if this continues our next generation would fail to know that there was such an ethnic tribe of people with so much colour.
Folk art is almost on the verge of extinction. Due to mass urbanisation this culture is almost in the end. Our new generation has very little knowledge on this scenario. This fine form of art can be helped spread throughout with the help of a photographer.
I was always fascinated in this form of art, which was one of many reason which drove me to this project. Then I started researching on the internet and started understanding the different form of folk art in different village. I found out the locations & noted down the time on which they indulge themselves in to art, took the visa for India and went for it.
Well at first I did not jump in and start taking photos. I went to each place two or three days before, met the people, shared my views with them as well. By the time they got accustomed with me & it became very comfortable for me to take the photos as well.
Portrait photography has always been one of my passion. I have spent almost many years for portrait photography. For this project i have spent a month.
I want to share the fact that since folk art is almost extinct, my work would give people the glimpse how amazing this form of art is.
Folk culture is something that has always intrigued me. Bangladesh and india is famous for its immense folk culture & during my travels initially as a photographer i became very fascinated with the culture. The important thing is to know their views on life & their society.
Aerialscape
Holla Mohalla is one of the biggest festival celebrated by the Sikh community. It's a one day Sikh festival which most often falls in March and takes place on the second day of the lunar month of Chett, a day after the Hindu spring festival Holi but sometimes coincides with Holi. Hola Mohalla is a big festive event for Sikhs around the world.
The words 'Hola Mohalla' would thus stand for 'the charge of an army. The festival was founded by Guru Gobind Singh, the tenth Sikh Guru. The Guru was in the midst of fighting both Aurangzeb of the Mughal Empire and the Hill Rajputs, and had recently established the Khalsa Panth. On 7 March 1701, Guru Gobind Singh started a new tradition by overseeing a day of mock battles and poetry contests at Lohgarh Fort. The tradition has since spread from the town of Anandpur Sahib to nearby Kiratpur Sahib and the foothills of the Shivaliks, and to other Gurdwaras around the world.
The beginnings and ends of shadow lie between the light and darkness and may be infinitely diminished and infinitely increased. Shadow is the means by which bodies display their form. The forms of bodies could not be understood in detail but for shadow.
Famously known as Banaras or Kashi, the holy city of Varanasi is located on the banks of river Ganga and is considered the holiest city in India owing to its location and history and the numerous temples in it. The city of temples or Banaras, as it is popularly known, is a holy city on the banks of the River Ganga. Said to have been discovered by Lord Shiva, this city has immense historical as well as religious importance. Not just a place where pilgrims throng to visit the temples, Varanasi is also the destination where most people come to spend their last days as it is believed that a person who dies in this sacred place can attain salvation. Varanasi is one of the oldest continually inhabited places of the world and oldest in India.
Gabura


Since we are using drones more often in our videos, we wanted to share our list of best drones for travel. We are a travel-focused site and channel, so our gear requirements favor portability over performance.
Drones come in a variety of styles and price points. While it may be tempting to buy an inexpensive drone, we recommend getting a drone that has a GPS lock/navigation and a gimbal. Cheaper drones without GPS are extremely difficult to control, as they will not lock or hover in place when the controls are released. Also, having GPS often gives you a fail-safe in the event that you lose connectivity with your drone. In these instances, sophisticated drones from manufacturers such as DJI will return the drone to the recorded home point.
Having a gimbal on the camera allows you to capture stable video. The gimbal absorbs most of the movement, even in windy conditions. Gimbals are sensitive components but are worth the extra cost. They add dramatic and cinematic effects to your footage.
For more information on traveling with drones, check out our video on the topic.
DJI Mavic Air : Our primary drone when traveling. The drone is easy to transport with its folding arms, and has an advanced avoidance system and sensors. The drone has a 3-axis gimbal camera that is capable of capturing 4K footage at 30 frames per second (fps). While we would prefer to capture video at 60 fps, we find the size, weight, and portability of the Mavic Air to outweigh the performance found in larger drones (like the Phantom Pro 4 and Mavic Pro).
DJI Spark : The first drone that we used. This drone lacks 4K video (i.e., it has a 1080p HD video resolution with a 2-axis gimbal), but is significantly cheaper than the Mavic Air and more durable. The drone is an excellent way to get started in aerial videography and photography. One key advantage of the Spark is its ability to perform a palm take-off and landing. This is especially useful when flying in wet and sandy terrain.
Spare Batteries (Mavic Air | Spark): One the biggest drawbacks of drones is their short battery life, especially on smaller drones. Having spare batteries is extremely important when traveling, especially since you may only have the opportunity to charge when back at your hotel.
Controller (Spark): If you plan to do any serious drone flying, you will need to carry a controller. While we fly our drone with visual line of sight (VLOS), we use a controller every time to the increased range and stability in connection. The controller also gives you much more precision which helps when capturing smooth video footage. The Mavic Air includes a controller, but it is an optional accessory with the Spark (unless you purchase the Fly More Combo).
PolarPro Filters (Mavic Air | Spark): These are like sunglasses for your drone's camera. We use the PolarPro Vivid Collection filters, as they are offer both neutral density and polarization. Depending on the condition outside, we will use a filter to allow the drone to record at a lower shutter speed, creating a more cinematic look in the video. The filters also provide another layer of protection for your drone lens.
Spare Propellers (Mavic Air | Spark): If you fly a lot, then you'll break propellers. There is no way around it. We've damaged propellers while flying close to trees and bushes, so its good to have an extra set on hand. Plus, they are extremely inexpensive!
Landing Pad: This is an extremely underrated drone accessory. In fact, I wish I had purchased one earlier when I started flying drones. Landing pads provide a safe spot to land your drone. This is critical in places where you may have wet or sandy conditions. Repetitive exposure to moisture and sand during landing will cause damage to your drone, so it's worth getting a landing pad to protect your investment. They also allow you to see your home point at a distance (when viewing from the drone's perspective).
LiPo Bag: Lithium polymer (or lithium-ion polymer) batteries are considered a hazard as they are prone to exploding or catching on fire. While the chance of it occurring is low, it is a best practice to transport your batteries in a LiPo bag. These bags are fire resistant and designed to contain any dangerous malfunctions that may occur.
Battery Charging Hub (Mavic Air | Spark): When you're carrying multiple batteries, you'll want a fast and convenient way to charge them. DJI's multiple battery chargers work great. The Spark battery charger will charge up to three batteries at once. The Mavic Air battery charger can accommodate four batteries, but will only charge one at a time.


With the stock market at an all-time high on mediocre growth, I keep trying to remind myself that equity prices are a leading indicator as investors bet on future corporate earnings.
Recently, I revisited J. Anthony Boeckh's book The Great Reflation, which was written in 2010 and is a thorough, well-written analysis of the long-run cycles experienced by the U.S. economy and the affects of financial crises and monetary policy on the stock market.
1. The global financial system will always remain flawed and subject to price inflation and bubbles, so long as it is based on fiat paper money. All anchorless fiat money systems are destined to suffer inflation and instability.
2. Stock market investors will be playing a cat-and-mouse game with the Federal Reserve for years to come, a problem caused by excessive private and public debt.
3. Deleveraging of the private sector bodes well for the transition process to the next long-wave cycle (2015 or later).
4. In the short term, deficits and extreme monetary expansion help the private sector repair balance sheets, but they cannot raise the standard of living for the average person.
5. Gold is a crowded trade (in the context of 2010), but is useful as an insurance/inflation hedge in portfolios. Gold is an emotional purchase. Financial/investment demand for gold differs greatly from consumption.
6. Long-term returns from commodities as an asset class are unreliable, and they trade in manias.
7. Any effective reform of the global monetary system anytime soon is unlikely. Greater price inflation is coming.
8. The stock market has proven to do well after long-wave troughs following major financial crises.
9. The long run in this investment world no longer exists. Wealth preservation and portfolio safety are critical.
10. Tactical asset allocation is the key to wealth creation and preservation.
Boeckh's research concluded that the long-run inflation-adjusted return of the stock market since 1929 (including dividends) averaged just less than seven percent annually.
In my opinion, point number four in the list above perfectly describes what's transpired in this stock market over the last few years. Extreme monetary stimulus works in providing liquidity and price inflation to financial assets like equities. The stock market appreciated substantially since 2009, but the wealth effect from it has yet to raise the standard of living for the average person.
And if decisive asset allocation is the key to wealth creation and preservation, the time must be getting very close for taking some money off the table. Or at the very least, with interest rates unfavorable for cash or bonds, risk exposure should be reviewed. The speculative fervor that exists in the stock market today will dry up when monetary policy changes.


28 0.9.8 or more recent installed on your system.
30 connections as well as certificate authentication for users and bots.
38 ./configure --disable-tls. You can't forcefully enable it though.
39 The configure script will look for OpenSSL at the default system locations.
44 custom one, as they take precedence over any system-wide paths.
51 TLS must be requested explicitly.
53 difference is that you must prefix the port number with a plus sign.
56 to TLS, using the starttls Tcl command.
62 the server list in the config file.
66 Botnet links between TLS-enabled bots will automatically switch to SSL.
68 negotiation takes place (the password is not send as cleartext anyway).
74 connection is deliberately aborted and no clear text is ever sent.
82 from the bot with /ctcp bot chat), consult the KVIrc documentation.
99 only contains eggdrop-specific information on the subject.
107 enter your certificate fingerprint with .fprint SHA1-FINGERPRINT.
114 new certificate to connect to eggdrop, depends on your irc client.
127 file containing Eggdrop's private key, required for the certificate.
130 Specify the filename where your SSL certificate is located.
131 if your bot will accept SSL connections, it must have a certificate.
134 maximum verification depth when checking certificate validity.
135 Determines the maximum certificate chain length to allow.
140 are used for verification. Both can be active at the same time.
150 enables or disables certificate authorization for partyline/botnet.
151 This works only for SSL connections (SDCC or telnet over SSL).
160 handle of the user/bot.
167 verification completely. A value of 1 enables full verification.
169 or expired certificates. Details are documented in eggdrop.conf.


text:
Christoph Thomas Walliser, född 17 april 1568 i Strassburg, död 26 april 1648 i Strassburg, var en tysk musiker.

Biografi
Christoph Thomas Walliser föddes i Strassburg. Walliser var kollega och musikdirektör vid Strasbourgs universitet och vid Vårfrukatedralen i Strasbourg. Han utgav en traktat över figuralsången (Strassburg, 1611) samt åtskilliga samlingar körer, däribland några ur Aristofanes Molnen. Han avled 1648 i Strassburg.

Referenser

Noter

Tyska kyrkomusiker
Tyska klassiska kompositörer
Tyska musiker under 1600-talet
Personer från Strassburg
Födda 1568
Avlidna 1648
Män
Ugglan


At Spark Hire, we have observed social sites like LinkedIn and Quora starting to offer users the ability to post videos. It has become pretty obvious the most engaging content on a social media site is its videos.
As we have been working with video interviewing for over five years, we have received this trend with open arms.
Beyond better utilization of social media, videos create more transparency between the public and a company. Further application of videos can create more personalized communication for an organization's candidates and new hires. This group not only likes watching videos, but prefers them to gain insight into company's systems and people.
We added video messaging to provide our customers with a tool that empowers them to control and personalize their communication with candidates, employees, and clients without the direct aid of a marketing team.
Video messages can be used at any point in the hiring process and even to more effectively communicate with current hires (i.e. onboarding, employee engagement, etc.). This can be done in one central place: Spark Hire's video messaging.
The benefits of our new feature capitalizes on these video-forward trends. But use of video messaging can be different between in-house recruiting teams and staffing firms.
Employers can use video messaging for all stages of hiring. Video messages add a level of personalization to your candidate experience and onboarding.
To begin, use personalized videos to introduce candidates to the job. This can be done by a hiring manager, so candidates begin to be familiar not only with the job but their potential boss.
Next, have hiring managers or team members record themselves encouraging applicants to move forward in the process. And even by sending a video message to present a formal offer.
Once a new employee has been hired, use video messages to onboard them. By sending a video message about the schedule of their first few days, new hires are more at ease on their start date.
Last, consider using video messages to personalize memos. Instead of having an email sent from HR, for instance, record leadership giving praise to employees. This makes employees feel special and engaged.
Staffing firms can use video messaging for communication to both candidates and clients.
First, record and include a video introduction of the firm (or team members) in email signatures. This gives clients a face to your brand and differentiates you from competing organizations.
Next, clients can record a video describing their open positions. Use these videos when presenting clients to candidates. It creates a more genuine image of the client for candidates.
By updating clients with video messages, touchpoints become more personalized and engaging. And the same can be said for updating candidates.
Video messages serve as a great point of differentiation when soliciting new business. They can also promote the innovation of a brand.
While these are just some ways that employers and staffing firms can take advantage of video messaging, there are many more use cases. We can't wait to see how creative our customers get with this.
Log in to Spark Hire and go to your video messages.
Choose whether or not the video message will be for general use of specific to individual recipients.
Record or upload a video and customize your supporting content.
Finalize your settings such as expiration time frames and recipients.
When viewed, video messages will appear on a landing page featuring your logo and colors furthering the branded experience for your candidates, employees, and clients.
Through email notifications, you can also see who is engaging with you video messages and use it to you advantage in following up on your communication.
As you begin to use this new feature, keep an eye out for enhancements. We're already working on a version 2 release so stay tuned!
Now that you know a bit more about Spark Hire's newest feature, why it was created, and how it can positively impact your brand, we invite you to learn more about it here.
If you are a Spark Hire customer, contact your Customer Success Hero for assistance with our video messaging feature. You can also view "how to" articles here.
Not a Spark Hire customer? Get a live demo with one of our product experts to see the new feature and the Spark Hire platform in action.


Short Desc is that it is a group of Adventure Lovers in Saputara.
What I Eat In A Day | Cheat Day Everyday?
Yahi hai uska description of second announcement !!!
This is the place for desc of My Nava Announcement !!!
What a good reply !!!
This is something else out of the world !!!
Content or desc of first Fressh Discussion !!!
Full Desc is that it is a group of Adventure Lovers in Saputara.Full Desc is that it is a group of Adventure Lovers in Saputara.Full Desc is that it is a group of Adventure Lovers in Saputara.Full Desc is that it is a group of Adventure Lovers in Saputara.Full Desc is that it is a group of Adventure Lovers in Saputara.


text:
WASHINGTON — Gold Star families, disturbed by the controversies over how President Trump relayed condolences to the families of fallen soldiers, are asking America's politicians on both sides of the aisle to stand down.
Their pleas follow statements this week from Trump and White House aides that created a storm for the president reminiscent of August 2016, when then-candidate Trump leveled criticism at the parents of a slain soldier and suffered a severe backlash. Now, again, families of military personnel killed in combat have increasingly found themselves swept up in the political fray.
"This is not what we want the story to be about. We want the story to be about our loved ones and what they represented to this country," said Ryan Manion. Manion's brother, First Lieutenant Travis Manion, was killed in Iraq in April 2007.
"Sometimes the president says things that are out of line, and I don't know that he recognizes that the things he says can be hurtful," said Manion, who runs a foundation to support military families that is named for his brother.
"There's no perfect way to make that phone call," Chief of Staff John Kelly said, adding that Trump had "bravely'' decided to console the families. Pablo Martinez Monsivais/Associated Press
The collision of politics and personal tragedy continued from the White House podium Thursday. White House Chief of Staff John Kelly, a retired Marine general, read an emotional statement to reporters, detailing what happened when his son was killed in Afghanistan in 2010. Kelly, who has emerged as the White House's go-to person in especially damaging press cycles, defended Trump's outreach to Gold Star families.
"There's no perfect way to make that phone call," Kelly said, adding that Trump had "bravely'' decided to console the families.
However, Kelly also lamented a changing culture, one in which the politicization of Gold Star families has become commonplace.
"When I was a kid growing up, a lot of things were sacred in our country," Kelly said. "The dignity of life was sacred, that's gone. Religion, that seems to be gone as well. Gold Star families, I think that left during the conventions over the summer."
But Kelly's statement itself had a political impact. It was made in a bid to quell a controversy that began Monday when Trump questioned how often Barack Obama made personal phone calls to the survivors of slain military personnel while he was president. The next day, after being criticized, Trump escalated the incident by defending his anti-Obama statements with a reference to Kelly's slain son, First Lieutenant Robert Michael Kelly.
"You could ask General Kelly, did he get a call from Obama?" Trump said Tuesday morning in a radio interview with Fox News.
Trump's statements invited scrutiny of his own performance in next-of-kin notifications, igniting another conflagration almost immediately. On Tuesday evening, reports emerged that Trump had told the widow of Sergeant La David Johnson, a combatant killed this month in Niger, that her husband "knew what he signed up for," which the family felt was disrespectful.
A Florida congresswoman, Frederica Wilson, heard Trump's conversation with Myeshia Johnson and went public with her criticism of Trump's tone. Trump responded by saying Wilson's account, which was supported by the Johnson family, was "totally fabricated."
"It is appalling what the congresswoman has done, and the way that she has politicized this issue," White House press secretary Sarah Huckabee Sanders said at Wednesday's press briefing.
Kelly also criticized Wilson for listening in on the president's phone call — though Kelly was listening in on the president's end. Kelly also said he did not fault Obama for not calling him after Michael Kelly was killed.
Some Gold Star families, even while criticizing Trump's clumsiness, wanted to move past the public debates.
"Just as there is no right or wrong way to grieve, there is no right or wrong way to express one's condolences; what matters is that we honor the life and the sacrifice, and provide support for all grieving families so no one has to walk such a difficult journey alone," said a statement from Bonnie Carroll, a Gold Star wife who founded a military survivor support network after her husband, Brigadier General Tom Carroll, was killed in 1992.
The outcry comes after an election cycle in which military families made political points on behalf of both parties. The mother of a soldier killed in Benghazi spoke at the Republican National Convention. Then a speech by a Gold Star family at the Democratic National Convention set off a national firestorm when Trump lashed out at the husband and wife, Khizr and Ghazala Khan.
Other presidents have gone to great lengths to avoid criticizing military families — even when they are critical of the commander in chief — but Trump stood out in that moment for his propensity to strike back.
In an interview with ABC News, Trump commented on Ghazala Khan's presence on stage, insinuating that her long periods of silence were tied to restrictive gender roles in Islam.
"If you look at his wife, she was standing there. She had nothing to say," Trump said. "She probably — maybe she wasn't allowed to have anything to say. You tell me."
"We want the story to be about our loved ones and what they represented to this country," said Ryan Manion. Manion's brother, First Lieutenant Travis Manion (center), was killed in Iraq in April 2007.Alex Wong/Getty Images/File 2010
Manion said the spotlight on Trump's actions overshadows the more important story — the lives of the soldiers and the cause they died to protect.
"What Gold Star families represent to this country should transcend politics," Manion said. Fallen soldiers "represent the men and women that are still here after their loved ones gave their lives in sacrifice to all of us."
Some of Trump's harsher critics say this is more evidence he holds little respect for America's military branches.
"Mr. Trump, stop. Please, just stop. Your actions and words on this entire matter of the fallen in Niger is disgraceful, and unbecoming of a President of the United States and Commander in Chief," said a statement from Karen Meredith, the Gold Star and Military Families coordinator for the liberal organization VoteVets.
Meredith's son, First Lieutenant Ken Ballard, was killed in Iraq in 2004.
"This is not about [Trump]. . . . It is about all of us who lost our loved ones in war," Meredith said. "For once in your life, at least pretend to know what empathy is. For once in your life, at least try to care about other people and their feelings."
Other Gold Star families said Trump is being unfairly criticized. Arnold Wright, whose son Dustin Wright was killed in the same recent raid in Niger, told reporters Wednesday that Trump's phone conversation with him was respectful.
In a CNN interview this week, a Gold Star father named Craig Gross said Trump's words are "basically being taken and misconstrued." Gross's son, Corporal Frank Robert Gross, was killed in 2011 in Afghanistan.
"I believe that if you interviewed him personally, one on one, you would find that he is very, very empathetic and very compassionate," Gross said on CNN. "Not only toward Gold Star families but also in regards to our active duty."
Though Johnson's mother and widow both characterized the president's phone call as "disrespectful," they have also asked for privacy.
In her only public appearance, Myeshia Johnson, Sergeant Johnson's pregnant wife, was seen hugging her slain husband's casket in tears.
On Monday, President Trump questioned how often Barack Obama made personal phone calls to the survivors of slain military personnel while he was president.Tom Brenner/New York Times
Astead W. Herndon can be reached at astead.herndon@globe.com. Follow him on Twitter @AsteadWH.


I bought chia seeds with an OU hechsher, and only after looking at it thought to ask if it should be checked. From what i can see and based on my limited knowledge, it seems "clean".
Personally I am not at all familiar with chia seeds, but I consulted with the OU, and their representative said that they are clean and there is no need to check them.


Using the video diary footage that I took during the expedition, BBC Wales have made a 30 minute documentary about my climb of Everest. It will be broadcast on BBC Wales but see below for ways you can watch if you'd like to.
BBC2W – On Top of the World – Tuesday 6th May at 7pm. BBC 2W is on digital satellite channel 991 outside Wales and 102 within Wales. The documentary should also be available online on BBC iPlayer for people to watch up to 7 days later.
Let me know what you think of it by posting a comment below.


We are looking for Intermission Team Skaters for the Cougars Men's and Women's Minor Hockey Night Games. These nights will include free giveaways during the game, autograph sessions with the team, team dressing room tour, pictures with your favorite player after the game and much more! Click below to sign up!


Travel dates? Check! Must-see list of attractions? Check! Now all you need to do is find a place to stay, narrow down all those great food options, and see about getting around. Right this way.
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The Greater Moncton Roméo LeBlanc International Airport is only a 10-minute drive from downtown.


Modux 1 round pond spot light is a very small low-voltage dimmable outdoor spot light with a solid metal base and stiff metal bracket for installing into water features. Fully adjustable, this spot light typically produces 85 lumens.
Clear or frosted lens cover. Standard cable length 3m - other lengths available on request .


text:
Functional Analysis of Cancer-Associated DNA Polymerase ε Variants in Saccharomyces cerevisiae
Stephanie R. Barbari, Daniel P. Kane, Elizabeth A. Moore and Polina V. Shcherbakova
G3: Genes, Genomes, Genetics March 1, 2018 vol. 8 no. 3 1019-1029; https://doi.org/10.1534/g3.118.200042
Stephanie R. Barbari
Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198
Daniel P. Kane
Elizabeth A. Moore
Polina V. Shcherbakova
For correspondence: pshcherb@unmc.edu
DNA replication fidelity relies on base selectivity of the replicative DNA polymerases, exonucleolytic proofreading, and postreplicative DNA mismatch repair (MMR). Ultramutated human cancers without MMR defects carry alterations in the exonuclease domain of DNA polymerase ε (Polε). They have been hypothesized to result from defective proofreading. However, modeling of the most common variant, Polε-P286R, in yeast produced an unexpectedly strong mutator effect that exceeded the effect of proofreading deficiency by two orders of magnitude and indicated the involvement of other infidelity factors. The in vivo consequences of many additional Polε mutations reported in cancers remain poorly understood. Here, we genetically characterized 13 cancer-associated Polε variants in the yeast system. Only variants directly altering the DNA binding cleft in the exonuclease domain elevated the mutation rate. Among these, frequently recurring variants were stronger mutators than rare variants, in agreement with the idea that mutator phenotype has a causative role in tumorigenesis. In nearly all cases, the mutator effects exceeded those of an exonuclease-null allele, suggesting that mechanisms distinct from loss of proofreading may drive the genome instability in most ultramutated tumors. All mutator alleles were semidominant, supporting the view that heterozygosity for the polymerase mutations is sufficient for tumor development. In contrast to the DNA binding cleft alterations, peripherally located variants, including a highly recurrent V411L, did not significantly elevate mutagenesis. Finally, the analysis of Polε variants found in MMR-deficient tumors suggested that the majority cause no mutator phenotype alone but some can synergize with MMR deficiency to increase the mutation rate.
DNA polymerase ε
mutator
Received November 25, 2017.
Accepted January 17, 2018.
Copyright © 2018 Barbari et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Volume 8 Issue 3, March 2018
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Differentially and Co-expressed Genes in Embryo, Germ-Line and Somatic Tissues of Tribolium castaneum
Humans and Chimpanzees Display Opposite Patterns of Diversity in Arylamine N-Acetyltransferase Genes
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