The bathroom in Madonna Inn Room 143: Rock Bottom features stones on every surface and a rock waterfall shower.
Room #143 at the Madonna Inn is called Rock Bottom, and features rocks on the ceiling, walls, and floor. What a surreal experience. And the Flintstones never had this much luxury - the bathroom in Rock Bottom features a waterfall shower. Step inside this amazing room in the 360 panorama of Rock Bottom. You can also see 360 panoramas of 3 additional rooms.


Hebrews 6:19 - " This hope we have as an anchor of the soul, both sure and steadfast..."
In the first years of adulthood you will make some of the most important decisions of your life, and you will establish friendships and habits that will help you in your future career, family and ministry. For this reason it's important to find a safe place to discover God's call on your life, and to learn more of His love for you. Anchored is a group of young adults who are learning to love God and love each other. With a strong emphasis on fellowship, worship and the Word, we hope you will leave Hope Chapel on Sunday evenings feeling filled up and ready for a new week!
While each gathering can be different because the Holy Spirit moves uniquely each week, there is always a coffee bar at 7:00pm, followed by worship, a message, and a time to respond personally.
We give each other a lot of grace, because God has done the same for us. Indeed, perhaps the best thing about this group is that it's made up of imperfect people pursuing a perfect God.
Anchored also meets outside of Sundays for sporting events, guy/girl nights out, hikes and eating at our group's favorite restaurants. And our annual retreat is a favorite time where we get away to hear from God in the southern California mountains.
We hope to meet you soon at Anchored!
©Copyright 2019 Hope Chapel Huntington Beach, All Rights Reserved.


text:
With the event tonight we wanted to share our latest blog post with you by our own Tyler Lyle…
Home With the event tonight we wanted to share our latest blog post with you by our own Tyler Lyle…
"I was just doing songs for the next rock album, and I decided that what always took me so long in the studio was the writing. I would get in there, and I just wouldn't have the material written, or it wasn't written well enough, and so I'd record for a month, get a couple of things, go home write some more, record for another month — it wasn't very efficient. So this time, I got a little Teac four-track cassette machine, and I said, I'm gonna record these songs, and if they sound good with just me doin' 'em, then I'll teach 'em to the band. I could sing and play the guitar, and then I had two tracks to do somethin' else, like overdub a guitar or add a harmony. It was just gonna be a demo. Then I had a little Echoplex that I mixed through, and that was it. And that was the tape that became the record. It's amazing that it got there, 'cause I was carryin' that cassette around with me in my pocket without a case for a couple of week, just draggin' it around. Finally, we realized, "Uh-oh, that's the album." Technically, it was difficult to get it on a disc. The stuff was recorded so strangely, the needle would read a lot of distortion and wouldn't track in the wax. We almost had to release it as a cassette."
–Bruce Springsteen, recalling the early stages of the recording of the album Nebraska, Rolling Stone Magazine interview, December 1984
There are musicians who fit perfectly in a time and place- those who embody the spirit of the days in which they live. Nebraska takes us beyond Bruce Springsteen's Darkness on the Edge of Town, beyond The River, where he stands in the middle of a paradox, like a guitar string suspended between life and death. Nebraska is a leap into the darkness. On this record,everything happens with dead certainty. There are no choices and no chances. We are as fixed as the course of a river. We are taken to see that Mansion on a Hill, but we know before the end of the second verse that the gates of hardened steel completely surround it, preventing us from ever entering. We ride along with a killer whose only explanation for the things he's done is "I guess there's just meanness in this world." We are the men poking the dead dog with a stick, hoping that if we do it long enough, it'll get up and run. Springsteen wrote this during a period of depression, (and a winter in New Jersey), so it's no wonder that the recordings made with the E-Street Band didn't make the record. It's lonely in the Darkness.
After Nebraska's commercial failure, (He never even toured on this album.), Springsteen returned to full band arrangements, moving away from killers, mobsters and losers and focusing on the working class, while paradoxically becoming "The Boss". His next album, Born In The USA, sold more than 15 million copies, and seven songs off the album were released as singles. Springsteen has sold over 61 million records in the US, but it's the little, dark acoustic record recorded in a bedroom in Colts Neck, NJ, in the early 80's that speaks to the darkness in us all. Hegel says the truth of the acorn is the tree. And while many Springsteen fans saw this demo-quality record as the acorn- a blueprint for something grand to come — we see it as the tree.
© ATL Collective 2021. All rights reserved.

redpajama_set_name: RedPajamaCommonCrawl


The world of daylily hybridization keeps growing more colorful, and over the next two weeks I'll introduce you to the top new daylilies from local hybridizers here in our own backyard.
That's right, some of the hottest and most respected daylily hybridizers in the nation can be found in east-central Wisconsin.
First up, Appleton teacher Nate Bremer, owner of Solaris Farms in Reedsville, hopes to make your new year a bit more extravagant with his Class of 2015.
Bremer's plants offer the ultimate in winter hardiness, born and bred here in a wide open, unprotected field where they must thrive or be culled from his collection. Bremer also breeds for extensive branching habit and high bud count, all of which create impressive, long-lasting clumps in the garden.
Here's a sneak peek at a few of Bremer's exclusive introductions for the upcoming growing season. Find more information, photos and Bremer's extensive daylily, peony and lilium offerings at www.solarisfarms.com.
Wildly dramatic, with a stunning chevron eye in deep, dark maroon, the monstrous blooms of this colorful, golden orange beauty will draw immediate attention anywhere in the garden. Tall, eye-catching and colorful, Bromance is a big, bold beauty.
With an unusual, spidery flower form and bright, cheery color, Yeti's Pride is another giant plant. Tall scapes boast the flashy, flag-like blooms in rich yellow. Thinner petals of great substance pinch and twist like massive ribbons, held high over the foliage.
A subtle beauty in soft yellow, it is the thin, almost transparent etched eye of slightly darker orange that catches the eye of those who examine the large flowers of this unusual daylily offering. An elegant, round flower form with reflexed petals displays the subtle wash of deeper color that forms the unusual eye zone.
A tall, stately beauty, much like its namesake state park, High Cliff offers bright rosy blooms with a deeper, dark eye and thin, ruffled edge. A brilliant green throat adds a dramatic touch to the large, flat blooms.
Elegant and seductive in creamy white with a small, bright bluish-purple eye zone, this plant offers beautiful, open-faced blooms. One of surprisingly few eyed whites available, this is a beautiful plant to add to your daylily collection.
The Weasel series continues to expand with this crazy double-flowering beauty that features just about everything. Massive double blooms come fully loaded with a striking pinkish-purple color, a darker chevron eye and a large lime green throat. Watch for even more "weasels" on the horizon.
Bremer introduces a second double-flowering plant this year with the understated Talking Heads, richly colored in beautiful, creamy yellow. The large, fluffy blooms are held on strong scapes, with classic high Bremer bud count.
My personal favorite of this year's introductions from Solaris, Grade Inflation features breathtaking blooms in fiery orange with a heavily ruffled, deeper red edge and striking eye zone. Surrounding each petal, the ruffling becomes even more complex, with a thin wire edge of matching orange creating a stunning effect.
A beautiful lavender purple with a thin deeper reddish ring surrounding a mysterious webbed or transparent eye, Spy Games is a mysterious bloom that requires deep inspection to fully appreciate the beauty and hidden treasures that can be found in every bloom.
In deep, rich purple, the large flowers of Sidewalk Chalk have many elegant, yet subtle secrets. A thin, chalky watermark, slightly whitewashed over the brightly colored flowers is barely noticeable unless you examine the big blooms closely. Soft midribs in chalky white also accent each petal.


text:
using System;
using NUnit.Framework;

namespace Potato.Core.Shared.Test.TestConfigCommand {
    [TestFixture]
    public class TestEncrypt {

        protected const String Password = "password";

        /// <summary>
        /// Tests that after encrypting the data the command will be nulled out.
        /// </summary>
        [Test]
        public void TestCommandNulledAfterEncryption() {
            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt(TestEncrypt.Password);

            Assert.IsNull(command.Command);
        }

        /// <summary>
        /// Tests a nulled output password will raise an exception
        /// </summary>
        [Test]
        [ExpectedException(typeof(ArgumentNullException))]
        public void TestNullPasswordParameterException() {
            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt(null);
        }

        /// <summary>
        /// Tests an empty password will raise an exception
        /// </summary>
        [Test]
        [ExpectedException(typeof(ArgumentNullException))]
        public void TestEmptyPasswordParameterException() {
            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt("");
        }

        /// <summary>
        /// Simple full test showing a command can be encrypted, then decrypted when done so in memory.
        /// </summary>
        [Test]
        public void TestEncryptedCanBeDecryptedInMemory() {
            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt(TestEncrypt.Password);

            Assert.IsNull(command.Command);

            command.Decrypt(TestEncrypt.Password);

            Assert.IsNotNull(command.Command);
        }

        /// <summary>
        /// Tests the integrity of the decrypted data
        /// </summary>
        [Test]
        public void TestEncryptedCanBeDecryptedInMemoryIntegrity() {
            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt(TestEncrypt.Password);

            Assert.IsNull(command.Command);

            command.Decrypt(TestEncrypt.Password);

            Assert.AreEqual(CommandType.ConnectionQuery.ToString(), command.Command.Name);
        }

        /// <summary>
        /// Tests the integrity of the decrypted data
        /// </summary>
        [Test]
        public void TestEncryptedCanBeDecryptedInMemoryIntegrityWithComplexPassword() {
            const string password = "G1cK6yn3muCor0kL1g";

            IConfigCommand command = new ConfigCommand() {
                Command = new Command() {
                    CommandType = CommandType.ConnectionQuery
                }
            };

            command.Encrypt(password);

            Assert.IsNull(command.Command);

            command.Decrypt(password);

            Assert.AreEqual(CommandType.ConnectionQuery.ToString(), command.Command.Name);
        }
    }
}

redpajama_set_name: RedPajamaGithub


text:
/*! Bing Search Helper v1.0.0 - requires jQuery v1.7.2 */

$(function(){
	// Attaches a click handler to the button.
	$('#search-btn').click(function(e){
		$('.mygallery').remove();
		$('#content').append('

');
		$('#not-found').remove();
		$('#load-image').remove();

		e.preventDefault();
		// Clear the results div.
		var query = $('#image-query').val();
		if (query){
			$('.message-area').append("");
			search(query);
		}
	});

	$("#image-query").keypress(function(e) {
	  	if ( e.which == 13 ) {
	    	e.preventDefault();
	   		$('#search-btn').click();
	   	}
	});

	// Performs the search.
	function search(query){
		// Establish the data to pass to the proxy.
		var data = { q: query, sop: "Image", market: 'en-us' };
		// Calls the proxy, passing the query, service operation and market.
		$.getJSON('php/bing_proxy.php', data, function(obj){
			if (obj.d !== undefined){
				var items = obj.d.results;
				if(items.length != 0){
					for (var k = 0, len = items.length; k < len; k++){
						showImageResult(items[k]);
					}
					loadJs1();
				}else{
				 	$('#load-image').remove();
				 	$('.message-area').append("
Not found
");
				}
			}
		});
	}

	// Shows one item of Image result.
	function showImageResult(item){
		var text = ["
",
						"
"+item.Title+"
",
						"

",
						"",
						"",
					"
"].join("");

		$('.album ol').append(text);
	}

	function loadJs1(){
		$.ajax({
			dataType: "script",
			scriptCharset:"UTF-8",
			url: "./js/jquery.tn3lite.min.js",
			success: function(msg){
				loadJs2();
			},
			error: function(){
				return false;
			}
		 });
	}
	function loadJs2(){
		$.ajax({
			dataType: "script",
			scriptCharset:"UTF-8",
			url: "./js/main.js",
			success: function(msg){
			},
			error: function(){
				return false;
			}
		 });
		 $('#load-image').remove();
	}
});


text:
Q: How does electricity travel through a set of resistors arranged parallel to each other? For calculating net resistance in a combination of parallel resistors we use 1/R=1/r1+1/r2+1/r3....but electricity must travel through least resistance. Then how does this relation come in use? I am definitely unaware of some concept on this. The least 'r' should be the net resistance instead of sum of reciprocals..

A: Because you may imagine that the electrons "pile up" in a queue to get through the smallest resistor, and when the queue is too damn long, this resistor is not anymore the easiest way to the other side for the lastcoming electrons.
A step by step thinking is this:

*

*The first electron arriving goes towards any resistor, since at this moment it doesn't know which is the smallest. It then reaches the resistor and starts to be slowed down.

*The next electron as well does not know which resistor is smaller, but at one resistor there is already an electron struggling to get through. They repel, so this second electron will not choose this resistor but any of the remaining.

*This continues, until one electrons arrives at each resistor.

*The next coming electron now has to choose. The resistor showing the smallest repulsion is the one where the previous electron has moved the furthest. This must be the weakest resistor, so here this next electron qeues up.

In the end, if one resistor is just half of the others, then 2 electrons will arrive here before one will choose to arrive at another one. This is the reason for more current passing through the weakest resistor.
At the same time you can also say that the resistors now share the crowd of electrons. If the weaker resistor was the only one in the circuit, then all electrons had to go this way. The queue would be long. The pressure from one side to the other is still just what the battery sets. E.g. 5 V. Now in this parallel setup, the pressure is the same (because the battery is the same) so the same rate of electrons will pass through the weakest resistor. But now there are also electrons passing through the other resistors. At a slower rate, yes, but this indeed looks like more electrons in total are passing through the parallel setup - the current is higher - than it would do through even the weakest of the resistors alone in a series setup.
This is an intuitive way to accept the fact, that the reciprocal-resistance formula results in an overall (equivalent) resistance that is smaller than even the smallest resistance in the set.

redpajama_set_name: RedPajamaStackExchange


Please allow approximately 60 minutes for your initial visit to Carey Chiropractic & Rehabilitation. There are essentially 6 components to the visit.
Registration- you may download all of our registration forms in order to save some time in the office. Click here to link to registration forms.
History- After you complete the initial registration process; we will begin to discuss your health concerns and past history.
Physical Examination- will be completed, which has two primary components: (1) neurological and orthopedic examinations to determine a clinical diagnosis and (2) assessment of your posture, muscle function, flexibility and spinal joint function.
Treatment Planning- The details of your diagnosis will be presented and discussed.
Treatment- Treatments in our office may include stretches, exercises, therapy modalities (electrical muscle stimulation, ultrasound, intersegmental traction and ice/heat packs), therapeutic massage and spinal manipulation/adjustment.
World renowned researcher of spinal pain (Karl Lewitt MD, PHD and head of Neurology at Charles University) has determined that joint dysfunctionis the key reflex associated with spinal pain. Thus, assessing and treating joint dysfunction is the first line of treatment.


OHMYGOODNESS Not only did I miss Donten ni Warau's eleventh episode< I also missed Sora no Method's. I sincerely apologize if you've been following these Sora no Method recaps weekly. Haven't done a double recap/review in a while but these two episodes seem to go together.
Somehow I feel like Nonoka understands Noel more than everyone… more than the audience anyway, though she's not revealing much.
GAH I wish Souta and Yuzuki would just make up. This stupid wish is wrecking everything, EVEN SIBLING LOVE!!! Souta is such a sweet brother. HIS wish to the saucer was for him to be friends with everyone, even Yuzuki. He supposes that the saucer's sparkles over the lake was Noel's way of granting Souta's wish. Yuzuki's wish was to be able to make up with all her friends after a fight. Souta muses that it's probably time they tried to grant Noel's wish since Noel's been working (I guess) to grant everyone else's wish. The semantics of this wish granting business is hella vague. This ambiguous supernatural force frustrates me =.=" Also, Noel takes things too literally… that chibi needs to learn figurative language, but then again, I think she's six… or something.
Everyone arrives at the observatory and you'd THINK things are going well as the credits roll cause things in the present day seem to repeat itself, including Shione's introduction into the group again and Shione taking pictures (cause I guess the pictures are there for when Noel disappears). BUT WAIT there's still like seven minutes to go and you just know weirdness is going to happen cause Noel suddenly says aloud that she needs to go home. I REALLY don't get why Shione is so attached to Noel. A trip to the hot springs makes you besties nowadays I guess…? Supposedly they're ALL attached to Noel because they all spent time and "bonded" with Noel though it doesn't really feel like it. Still, I suppose it's a bit emotional with everyone crying.
Noel. You're a saucer. How have you never witnessed a meteor shower?!?? You live in the sky. You're magical. HOW?!?
Hold up. Maybe it's a good thing I skipped last week's review so both episodes could tie together in these pre-finale thoughts. This is JUST WEIRD. Is it a time skip? Alternate dimension? It's gone from summer to snow to time skip backwards ohmigosh WUT?!?
Well now this is just stupid cause NO ONE remembers their friends with each other AT ALL. Noel disappeared. The saucer disappeared.
Somehow, Nonoka finds her way back to the observatory but no one's there. She returns home in a despondent state. No one has any recollections of their time with Noel except Nonoka so Nonoka resolves to record everything she knows about Noel.
Ohhhhh they played the dream card. WHYYYY WAIIIIIII. This is 2014. No one uses that stereotypical plot device anymore geezus.
It can't be a dream now because Shione remembers so what is this series actually trying to do? I hope the writers weren't shabby enough to think we'd buy this time lapse happy friendship ending because it certainly has not provided any semblance of closure yet.


Studies show that people who carry extra weight sleep less than people with lower body mass indexes (BMIs).
Sleep apnea, acid reflux, and back pain are three common sleep issues that are related to being overweight or obese.
Men are particularly at risk of developing sleep apnea.
Extra body weight has been associated with many health conditions, such as heart problems and a low sperm count. But being overweight or obese can affect another crucial area of your health: your sleep habits. Many studies show that people who are overweight and obese sleep less than those with lower BMIs.
Here's how (and why) being overweight affects your sleep habits — and what you can do to finally catch some much-needed Zs.
You may develop sleep apnea.
According to neurologist Dr. Gregory Carter of the UT Southwestern Medical Center in Dallas, Texas, men have a one in four risk of developing sleep apnea. The condition causes people to stop breathing for up to 30 seconds at a time while they're asleep. In extreme cases, this can happen for upwards of 60 times per hour, as MensHealth.com has previously reported. Often, guys with sleep apnea don't even know they have it, but the symptoms include snoring, fatigue, and dry mouth.
The likelihood of developing sleep apnea increases if you have a BMI higher than 30, says Carter. (For reference, a "normal" BMI is between 18.5 and 24.9.) That's because obese guys generally have more fat in their necks, which means they tend to have more fat lining the airways in the back of their throats. This makes the passage narrower and inhibits airflow, causing breathing problems, Carter says.
Sleep apnea can be dangerous: a 2008 study found that people who had severe sleep apnea were five times more likely to die of cardiovascular problems compared to those who did not. This is because repeatedly depriving your heart of oxygen causes lasting damage, says Carter.
If you have sleep apnea, Carter recommends losing weight or quitting smoking. If you have more severe sleep apnea, you can use a Continuous Positive Airway Pressure (CPAP) therapy device, which helps open blocked airways, according to Mayo Clinic.
Roughly 80 percent of Americans will have lower back pain at some point in their lives, according to the National Institute of Health. "Individuals who are carrying too much weight of course have more stress on their backs," says Carter.
People who are obese tend to experience chronic back and joint pain, or pain that lasts longer than three months. Research by the National Sleep Foundation indicates that chronic pain sufferers often can't find a comfortable position, so they lose about 42 minutes of sleep each night.
If you do suffer from back pain, a good mattress can help. Replacing overly soft or worn-out mattresses with a medium-firm to firm option can reduce lower back pain.
Acid reflux occurs when stomach acids flows into your esophagus, explains the Mayo Clinic. Doctors believe it's caused by extra stomach fat causing the muscle separating the stomach and esophagus to loosen, allowing acid into your esophagus. Smoking, drinking alcohol, and lying down within three hours of eating can trigger or exacerbate the problem.
Carter says that if you have acid reflux, the acid could creep into your esophagus up to several times per hour while you're sleeping, which may cause you to wake up throughout the night. "That certainly disturbs sleep," he says. Fortunately, drinking less alcohol and not eating close to bedtime can lower your chances of developing the condition.
All of these conditions have been linked to excess weight — which means that losing weight can help you manage symptoms. This doesn't mean you need to jump on the Keto bandwagon: you can start by making small dietary changes, such as slightly reducing portion size and eating one serving of oats a day, which are loaded with fiber to keep you full, suggests gastroenterology clinical researcher Bethany Doerfler of Northwestern's School of Medicine.
Be sure to eat three to six servings of vegetables and three to four servings of fruit per day — and of course, cutting out processed snacks will help reduce calories.


text:
Under Review: 'Wikileaks'
— by BEV QUESTAD —
News releases are out today about Pfc. Bradley E. Manning's 2007 video documenting an apparent US massacre of at least 12 civilians in Bagdad. He's been charged with eight violations of the U.S. Criminal Code for illegally transferring classified data to Wikileaks.org. Is he a traitor or a hero?
Mixed in this embarrassing mess for the Pentagon is white-haired, lean, soft-spoken Julian Assange. He has proudly joined the ranks of Daniel Ellsberg in being referred to as the most dangerous man on earth.
It's all about transparency in the Age of Information.
Assange is the editor-in-chief of Wikileaks.org, a Web site devoted to exposing secrets. Any country, any organization, any person. Primary documents, including videos, are downloaded anonymously through digital dropboxes. This is where Manning went, motivated by conscience, to download his video and government documents, probably hoping that the promise of anonymity and legal protection would provide true sanctuary and affect a change in current military practices.
Wikileaks began in the '80s in Melbourne, Australia, as an international hackers' club called International Subversives. It eventually grew into a powerful site challenging governments, organizations and businesses throughout the world. In the last three years it has uncovered human rights violations at Guantanamo Bay, political murders in Africa and banks laundering money through offshore tax havens. Both Amnesty International and 'The Economist Magazine' have given it media awards.
All of this information is covered in the documentary short, narrated by Andrew Fowler, ABC News foreign correspondent, called "Wikileaks" released for free viewing on Journeyman Pictures this month.
http://www.journeyman.tv/?lid=60967#60741
Comments from Daniel Ellsberg, famous for the 1971 Pentagon Papers leak, are sprinkled throughout the film. At one point, regarding US policy, he says, "We're being lied to and led into a hopeless war."
Ellsberg goes on to point out that in the last 17 months the Obama administration has "outdone previous administrations in pursuing leakers. The Obama administration, very briefly, is as secretive as the Bush administration … and in many cases has gone beyond Bush."
Manning's video of the civilian street casualties taken from a US helicopter in Iraq three years ago and downloaded to Wikileak's site, is shown in this film. Unarmed civilians are walking down a street. Amongst them are two Reuter's photographers. We are informed that the camera one of the journalists is carrying is mistaken for a rocket-propelled grenade launcher by the air crew.
A command by an immature voice tells the gunner to "take 'em out." The road is strafed, bodies drop. Rescuers quickly arrive to offer help, including a man driving his children to a tutoring lesson. They are all "taken out" as well.
Then it is revealed that U.S. policy regulations stipulate that when one person on a street comes in the line of fire, all people on the street must be killed.
You might think the U.S. Army is investigating this sad incident because of the mistaken command to mow down a photographer carrying camera equipment. However, the probe is not about the murders but about who leaked the information.
A returning soldier touring America with a group of Afghanistan vets repeatedly explains to audiences that incidences like this are not uncommon. For soldiers like him, they can't live with it and are going out to speak against the war.
In addition, "Live from Paris" reported today that 90,000 secret documents on the war in Afghanistan have been posted on Wikileaks. The U.S. considers this an illegal breach of national security, but Wikileaks plans to release 260,000 more documents.
Interestingly, Wikileaks, based by permission in Iceland, appears to be protected from prosecution because the Icelandic government has written legislation declaring itself a protected offshore publication center.
Time magazine has reported that the concept behind Wikileaks " … could become as important a journalistic tool as the Freedom of Information Act."
Julian Assange states the Wikipedia purpose, "What we want to create is a system where there is guaranteed free press across the world – the entire world. That every individual in the world has the ability to publish material that is meaningful. We are kept honest by the fact that we release primary source material… readers can check what the primary source says." (www.youtube.com/watch?v=kCmjmDXp7TI).
The bottom line is that "Wikileaks" is the most important film you can watch, at your own convenience on your own computer, this week. After viewing it let IJM know — is 22-year-old Pfc. Bradley E. Manning a hero or traitor?
Producer: ABC Australia
Featuring: Andrew Fowler, Julian Assange, Daniel Ellsberg and others
Distributor: Journeyman Pictures
Time: 24 min 09 sec
Released: July 5, 2010
Web site viewing: www.journeyman.tv/?lid=60967#60741
Follow It's Just Movies on Twitter at http://twitter.com/ItsJustMovies.
Posted 28 Jul 2010 by Bev Questad in Reviews
← Trailer Talk: 'Sucker Punch'
See Josh Hutcherson's 'Spider-Man' Audition →
6 Comments Add Yours ↓
Adam Poynter #
Not so sure if I'm very interested in this one, I mean I've seen a ton of documentaries and liked a lot of them… this one just doesn't seem to tickle my fancy!
Paul Helton #
We prosecute war atrocities all around the world as violations of the rights of mankind. This would simply be another of those. This is why I refuse to put a "tag" on Manning. As with anything human, it's not that black and white without knowing all the facts. No group is above the law of basic human rights. Period. (At least that is what my moral code says for me.)
Mark Fritch #
I cannot say whether or not that Manning and Assange are patriotic or not. At this point, I'm not sure that there is anything about the US being in either Afghanistan or Iraq that is patriotic other than one human being caring for another in horrific times; be they Afghan or a couple of enlisted men. That seems to be about all the humanity that exists there.
Yes, this leak is going to create some hell for those in power. It will call them to step up and be accountable for their actions. They are working on our behalf as US citizens and it is ultimately our responsibility for what they do. You may not like that idea, but I suggest that it is the truth. When enough of us are sufficiently angry about what is going on, the needed changes will be made in Washington, D.C. Until then, we will continue to get more of what we're already getting.
Are people pissed off and angry? You bet they are! There is a place in the Bible where Jesus is telling the people, "….and the truth will make you free." They left out a very important preface to that statement which is, "But first it is going to really, really piss you off!" That's the nature of the truth. It will cause upset, but it is upset that has the possibility of bringing us to the truth of what needs to be done.
I'm all in favor of the truth. We have not had "the war in our living rooms" like it was during the Vietnam War. We are anesthetized. We don't know what is happening and we want to hide from it. It is painful to know the truth. I know that there are downsides to telling the truth in the short-term, but in the larger picture, the truth will make us free.
For now I will refrain from judging a person to be patriotic or not. What we need is the truth, the whole truth and nothing but the truth. We need to know "what's so," so that we can get to "what works." Once we have the "what works" we can begin doing what needs to be done.
I just read Bev's Bess O'Brien review. It is a totally unrelated article, but when Bess was asked the three most important things she learned from making a movie about the most poignant issues for teens today, one of the three most important things she learned was, "That telling the truth can create change."
trish.henderson@hotmail.com #
Good God! What a screwed up mess we have created.
Coming from the Vietnam era, when the US govt lied to the people but the press brought the war into our homes every night (back when families sat down before dinner and watched the news together)- it is deja vu, isn't it?
I thought the purpose of studying history was to avoid repeating our mistakes.
As for hero or villian, I don't feel I have the right to judge. I feel my responsibility is to vote my conscience.
To ignore what is going on elsewhere by saying, "It's not my problem, I can't do anything about it." is a croc.
Bev Questad #
8/28/10 CNN Update (excerpts)
Manning had top-secret clearance as an intelligence analyst with the Army while he was stationed in Iraq. He was detained in June and sent to the U.S. base in Kuwait for his connection to the release of the classified U.S. military combat video showing the shooting death of Iraqi civilians and two journalists in 2007 by a helicopter gunship.
The Army is trying to trace who Manning's contacts were and on what computer servers he had to possibly access a plethora of information from U.S. military documents to Department of State cables, according to the official.
Manning has not been cooperating with Army investigators; the official said Manning has invoked the Fifth Amendment and is refusing to answer questions from investigators.
Manning is still being held by the U.S. military in Kuwait.
Manning could be additionally charged as the Army's investigation continues, according to the official. At the moment, he has been charged with illegally transferring classified data onto his personal computer and adding unauthorized software to a classified computer system in connection to the leaking of the helicopter video.
He has also been charged with communicating, transmitting and delivering national defense information to an unauthorized source and disclosing classified information concerning the national defense with reason to believe that the information could cause injury to the United States in connection to the leak of the same video.
Tweets that mention Under Review: 'Wikileaks' - -- Topsy.com 18 08 10
The Fifth Estate: The First Trailer / It's Just Movies 17 07 13


Rick Keene Music Scene – Wake Up Music World, It Is The 'Crack of Dawn' !
In a music galaxy far, far away – a Canadian band altered the airwaves and sent Canada into an entirely different universe.
Crack of Dawn were the first black band to be signed to a major label (CBS) in Canada.
Otis Redding's producer discovered them and the band sold more albums than Earth, Wind and Fire in Canada. They were headed for stardom yet they disappeared.
Please listen below to my chat with Arvin Jones (Sax) and Trevor Daley (Trombone) as they explain Crack of Dawn.
Visit Crack of Dawn Here !
Previous Previous post: Rick Keene Music Scene – April Wine Co – Founder Jim Henman's Family Affair !
Next Next post: Rick Keene Music Scene – BackTraxx; Bringing Hairspray and Rock n Roll Back from The Eighties !


Enter Your Name And Email To Download My 100% Free EBOOK/Report!
GET ON THE VIP LIST !
This Bonus Ebook will also be Emailed as a Gift to you When you Become a VIP !
Enter Your Name And Email To Download My 100% Free Ebook/ Report!


text:
Google Nexus 5 – First Impressions on Software and Hardware
I received my Nexus 5 today and here are some quick first impressions.
First a quick recap of the specifications of the latest Nexus flagship phone made by LG for Google:
4.95-inch 1080p Full HD display (445 ppi)
Qualcomm Snapdragon 800 processor clocked at 2.3 GHz
8-megapixel rear camera with the LED flash and Optical Image Stabilization (OIS)
OS: Android 4.4 KitKat OS, Google's latest flavor of Android
The first thing you notice when you pick up the Nexus 5 is its size, or should I say lack of it. For a 5″ phone, it feels incredibly comfortable to hold. The slight curves along the back edges along with the soft touch back, similar to the Nexus 7, serve to make the phone very ergonomic. You can reach all four edges of the phone without having to move it around too much in your hand.
The phone is extremely fast and the processor can handle everything you throw at it. I didn't see any lag whatsover when opening apps, switching between apps or scrolling in the web browser. The Nexus 5 handled everything with ease. In normal use, the Nexus 5 seems to be both faster and smoother than the Nexus 4 and the Samsung Galaxy S3 that I was able to compare it to.
The 5″ 1080p screen@ 445 ppi is extremely crisp and bright and makes for a amazing viewing experience. You can't make out any individual pixels on the screen even if you looked for it closely. Overall, it is among the top screens currently on a smart phone. HD videos on Netflix and Youtube look great. This phone could easily replace your tablet as a video player on your plane rides. Overall I am really impressed with the screen on this device.
The one thing that has disappointed me so far is the speaker on this phone. It is among the softest speakers I've ever encountered on a smartphone. Even at full volume, the sound is less than half as loud as on an iPhone 5 I compared it against. When you are outdoors or driving in a car, I would expect not to hear much at all through the speaker. Hopefully this is something Google can fix with a software update in the future.
The Nexus 5′s predecessor, the Nexus 4, was widely criticized for its middling camera performance. In the limited time I had with the Nexus 5, it seemed to take decent pictures, both indoors and outdoors. The OIS system certainly helps in low light conditions to compensate for hand shake. However the camera app overall seems to be on the slow side. It take a second or two to open and when you take pictures, sometimes the auto focus is slow to get a lock. This could result in people missing out on important moments if they are trying to take action shots of a fast moving object, pet or a kid. There is also no burst mode in the camera, which is suprising for a high end phone in 2013. Again, hopefully Google can improve the camera performance with future software updates.
The phone runs on Android 4.4 KitKat as expected. With KitKat, Google has gone with a 'flatter' look this time around and the OS looks very similar to iOS in many areas. When you pull down the notification screen, it is translucent, just like iOS 7. There are lots of things to like about KitKat such as – the Google Now integration throughout the OS, the local business/phonebook type search integrated into the Phone app, the new Hangouts app that also doubles as the messaging app, and the new full screen mode that is available with certain apps.
At $349 for the base 16GB model, the Nexus 5 is an incredible value for money. That is a lot of smartphone for that price. The 5″ 1080p screen combines with the new flatter clean KitKat OS to provide one of the best, if not the best, Android experiences on a phone. I strongly recommend anybody that is currently in the market for a smartphone to give the Nexus 5 a long hard look.
A full hands-on review of the Nexus 5 is in the works and should be released very soon and will include detailed testing of all aspects of the phone. But for now, you can't really go wrong paying $349 for this latest Nexus device.
Best Google Nexus 5 Smartphone Cases & Covers
Google Nexus 5 with Sprint available for $49.99 on Contract, $449.99 off-Contract!


text:
Posted in: Main.	Tagged: Irinotecan manufacturer, Rabbit Polyclonal to STEAP4.
Supplementary Components1. neuronal firing patterns that support goal-directed storage and behavior. Working storage (WM) may be the ability to briefly store and procedure information that's relevant for completing goal-directed activities. Predicated on the deep behavioral functionality deficits in complicated Irinotecan manufacturer spatial WM duties after dentate gyrus (DG) lesions, the projections of DG granule cells to CA3 through the mossy fibers (MF) pathway have already been regarded Rabbit Polyclonal to STEAP4 as crucial for WM with delays of tens of secs to hours1. The discovering that spatial firing patterns of dentate cells present pattern parting2,3 provides resulted in the recommendation that DG projections to CA3 support storage by marketing the era of distinctive hippocampal firing patterns. However, the DG-CA3 network is normally seen as a repeated cable connections, both straight between CA3 cells aswell as even more between dentate granule cells indirectly, hilar mossy cells, and CA3 cells4,5. These thick indirect and Irinotecan manufacturer immediate repeated pathways in the dentate-CA3 network type associative circuits, which were suggested to become critical for keeping complicated spatial WM6, 7. Furthermore, the CA3 area C separately8,9 or by modulation in the CA2 area10 C is normally considered to initiate sharp-wave ripples (SWRs), which propagate from CA3 to CA1 then. SWRs are prominent hippocampal network oscillations (150C250 Hz) that are found during sleep aswell as during intervals of immobility in awake behavior11. SWRs are seen as a short rounds of elevated neuronal activity where time-compressed sequences are replayed, which match sequential activity patterns that are found on a longer period range in behavior12C16. The reactivation of sequential activity patterns during SWRs is normally considered to underlie procedures important for storage such as loan consolidation and retrieval17C19. Specifically, it's been proven that selective reduction of awake SWRs in hippocampus impairs potential route preparing20 and behavioral functionality within a spatial WM job21. This shows that SWR-associated reactivation of hippocampal cell ensembles during ongoing behavior is normally a potential system for preserving representations of kept items for make use of in planning upcoming options in WM. There is certainly proof that SWRs usually do not take place during intervals of low DG granule cell activity22 and, conversely, that CA3 and hilar neuronal activity is normally elevated in parallel during SWRs8C10,23,24. These correlations, alongside the results that dentate and SWRs inputs to CA3 are both crucial for spatial WM, led us to research whether DG inputs to CA3 may donate to spatial WM by results on SWR era and on ripple-related neuronal firing patterns. In an activity where DG is essential for WM functionality, we therefore documented neuronal firing patterns in DG and CA3 and analyzed to what level CA3 network activity patterns and ripple-associated firing patterns of specific CA3 cells had been reliant on DG inputs. Outcomes Dentate granule neurons had been essential for spatial Irinotecan manufacturer WM in the 8-arm radial maze We initial confirmed our version from the spatial WM job was reliant on the DG. Rats (= 16) had been trained to execute an 8-arm radial maze job in which praise was offered by the end of every of the hands (Fig. 1a). In the initial Irinotecan manufacturer phase of every trial, four hands had been sequentially presented inside a pseudorandom order (forced phase). In the second phase, all arms were made accessible such that the animal experienced to remember and choose the arms where incentive was still available (choice phase). The optimal strategy is definitely to visit each arm only once per trial. A trial with at least one.
Posted in: Main.	Tagged: Rabbit polyclonal to DDX3, STA-9090 small molecule kinase inhibitor.
Infection by individual immunodeficiency pathogen (HIV) causes the?obtained immune deficiency syndrome (AIDS), which includes devastating effects in the host disease fighting capability. tissue. Extracellular nucleotides and nucleoside are signaling substances that act within an autocrine and paracrine method. Under tension, cells discharge adenosine triphosphate (ATP) towards the extracellular moderate, which activates P2 purinergic receptors triggering an inflammatory response. ATP amounts are managed by purinergic enzymes: E-NTPDase (EC 3.6.1.5; Compact disc39) changes ATP into ADP (adenosine diphosphate) and AMP (adenosine monophosphate) and E-5-nucleotidase (EC 3.1.3.5, CD73) converts AMP to adenosine. Adenosine suppresses the proinflammatory response and promotes an anti-inflammatory response through P1 purinergic receptors [1]; this shift ensures protection against tissue damage [2]. However, accumulation of adenosine prospects to immunosuppression in malignancy [3, 4] and contamination [5, 6]. Adenosine deaminase (ADA) (EC 3.5.4.4) controls the extracellular levels by converting adenosine into inosine [6]. A delicate balance is sustained by restraining inflammation while containing excessive immunosuppression. The first cases of acquired immune deficiency syndrome (AIDS), a consequence of human immunodeficiency computer virus (HIV) contamination, appeared in the early 1980s. Since then, research has come a long way unveiling major aspects of HIV pathogenesis along with developing diagnostic and monitoring tools, as well as effective antiretroviral therapy. Nevertheless, HIV genetic variability and host response evasion mechanisms are major difficulties for vaccine development and the complete eradication of the virus. HIV targets immune cells by infecting them or indirectly causing systemic changes which will have an effect on their function directly. Despite effective suppression of viremia, chronic irritation and immune system activation persist indicating that immune system function isn't totally restored by antiretroviral therapy [7, 8]. The goal of this paper is normally to examine the user interface between adenosine signaling as well as the immunopathogenesis of HIV an infection and discuss the consequences of adenosine deaminase activity over the HIV-induced immune system dysfunction. Adenosine STA-9090 small molecule kinase inhibitor immunosuppression and pathway Adenosine-mediated immunosuppression could be helpful in inflammatory illnesses such as for example autoimmunity, cancer, and an infection, marketing tissues regeneration and protection [9]. Actually, low concentrations of adenosine are located in the extracellular environment in physiologic circumstances. Upon hypoxia, injury, inflammation, an infection, or other notable causes of tension, adenosine is created because of ATP dephosphorylation [10C12]. Extracellular adenosine is normally generated via the?CD39/Compact disc73/adenosine pathway, which is activated by high degrees of extracellular ATP. Adenosine interacts Rabbit polyclonal to DDX3 with adenosine receptors, known as P1 receptors, in various types of cells in a number of tissues, such as for example heart, human brain, and disease fighting capability. A couple of four known types of P1 receptors, A1, A2A, A2B, and A3 [12]; all are expressed in immune system cells [9]. A2A receptors are fundamental players in the STA-9090 small molecule kinase inhibitor immunomodulatory activities of adenosine to STA-9090 small molecule kinase inhibitor keep an equilibrium between irritation and suppression of overactive immune system cells [13]. Activation of A2A receptors downregulates the discharge of proinflammatory mediators and upregulates the discharge of anti-inflammatory regulators. A2A receptor inhibition impacts the immune system STA-9090 small molecule kinase inhibitor response, from antigen display to T cell activation, extension, and function [14]. A2A receptors are even more from the suppressive/anti-inflammatory ramifications of adenosine straight, while A2B also serves as an anchoring molecule to ADA and increases immune system responses [15]. A significant mechanism mixed up in immunosuppressive ramifications of adenosine may be the creation of cyclic AMP (cAMP) by adenyl cyclases (AC). cAMP modulates several processes including the immune response as it influences function, proliferation, and activation of immune cells. Improved adenosine levels raise cAMP production via A2A and A2B receptors, which regulate its own release in immune cells. Elevated levels of cAMP, upon inflammatory and harmful stimuli, are known to have immunosuppressive effects [16, 17]. Adenosine effects the function, proliferation, and activation of immune cells, modulating and polarizing immune responses..
Posted in: Main.	Tagged: CAL-101 enzyme inhibitor, Col4a2.
Supplementary MaterialsAppendix EMBJ-37-139-s001. Glut?=?0.2% glutaraldehyde. Staining of nucleic acids after fixation. The propidium iodide sign in fibroblasts was considerably higher for examples set with glyoxal pH 4 (hybridization (Seafood) to get a target that's often utilized as a typical in such tests, glyceraldehyde 3\phosphate dehydrogenase (GAPDH). For the propidium iodide staining, the GAPDH sign intensity was considerably elevated by glyoxal at pH 4 (Fig?3B). To check whether identical results connect with lipids also, we immunostained cultured cells for phosphatidylinositol\(4,5)\P2 (PIP2). The strength from the immunostaining was considerably larger after glyoxal fixation (Appendix?Fig S8). The more powerful fixation induced by glyoxal is actually a concern for tests counting on enzymatic tags, like the SNAP\label (Xue and mouse (Appendix?Figs S16CS18). We didn't observe any problems in the antibody penetration in such cells, as opposed to fixation by, for instance, glutaraldehyde (as talked about in the Introduction). Glyoxal provides higher\quality images in immunostaining for many different laboratories The glyoxal fixation procedure established above was then tested in 11 different laboratories, in four countries (Germany, Sweden, United Kingdom, and United States). We present the results in alphabetical order. The Boyden laboratory (MIT Media Lab and McGovern Institute, Departments of Brain and Cognitive Science and Biological Engineering, Cambridge, Massachusetts, USA) tested nucleoporin 160 in conventional immunostainings of cell cultures and found that fixation with glyoxal at pH 4 resulted in brighter images than those obtained with PFA fixation. The samples exhibited similar morphology (Fig?5). Open in a separate window Figure 5 Comparison of immunostaining NUP160 after fixation with either PFA or glyoxalHeLa cells were stained for the nucleoporin complex protein NUP160 after fixation with either PFA, glyoxal pH 4 or glyoxal pH 5. Fluorescence intensities (fold over background) were compared and are shown in the Col4a2 graph. The quantification of fluorescence signals shows that glyoxal pH 4 fixation allows for significantly brighter stainings. hybridization Fluorescence hybridization (Fig?3B) was performed using the QuantiGene? ViewRNA ISH Cell Assay kit (Affymetrix #QVC0001), according to the protocol provided by Affymetrix. In short, cultured rat hippocampal neurons were fixed in one of the tested fixatives for 10?min on ice and for another 20?min at room temperature. After a washing step, the cells were incubated in the provided detergent solution, followed by probe hybridization for 3?h at 40C (using standard probes for GAPDH, provided with the kit by the manufacturer). Afterwards, the samples were washed in the provided wash CAL-101 enzyme inhibitor buffer, and signal amplification was done by incubating the samples in pre\amplifier and amplifier solution for 30?min each at 40C. Label hybridization was done as well for 30?min at 40C using Cy5 as dye. After cleaning in clean PBS and buffer, the samples had been inlayed in Mowiol and imaged using an inverted Nikon Eclipse Ti\E epifluorescence microscope. Transferrin, LysoTracker?, and cholera toxin uptake assay Live imaging of transferrin (combined to Alexa Fluor 594, Thermo Fisher #T133433) and cholera toxin CAL-101 enzyme inhibitor subunit B (combined to Alexa Fluor 555, Thermo Fisher #"type":"entrez-nucleotide","attrs":"text message":"C34776″,"term_id":"2370917″C34776) uptake during fixation (Appendix?Fig S4) was completed in COS\7 and HeLa (from the Leibniz Institute DSMZGerman Assortment of Microorganisms and Cell Culture) cells. The cells, plated on PLL\covered coverslips, had been incubated in 25?g/ml transferrin or 1?g/ml CAL-101 enzyme inhibitor cholera toxin at 37C for 10?min. Afterward, the cells had been cleaned in pre\warmed COS\7 cell Ringer and had been imaged. A focused solution of every fixative was put into the Ringer so the final focus of fixative was 4% for PFA and 3% for glyoxal. The cells had been imaged through the 1st 10?min of fixation using the inverted Nikon Eclipse Ti\E epifluorescence microscope. The imaging of transferrin and LysoTracker uptake at different period factors during fixation (Appendix?Figs S2 and S3) was done in HeLa and COS\7 cells. The cells had been incubated in the particular fixative for 3, 5, 10, 15 and 20?min in 37C towards the addition of 25 prior?g/ml transferrin Alexa594 or 50?nM LysoTracker Crimson DND\99 (Thermo Fisher #L7528). Each test was incubated in the fixative and transferrin/LysoTracker for 20 even more min. The cells were washed with PBS and inlayed in Mowiol then. The samples had been imaged having a confocal TCS SP5 microscope (Leica). Lipofectamine transfection of COS\7 cells, HeLa cells, and BHK cells For the imaging of preservation of varied GFP\tagged proteins and constructions (Appendix?Figs S6) and S5, COS\7 fibroblasts or HeLa cells were transfected having a TOMM70 build from 3rd\instar larvae neuromuscular junctions 3rd\instar larvae (Appendix?Fig S16) were dissected in regular Drosophila moderate as.
Data Availability StatementAll relevant data are within the paper. and a
Posted in: Main.	Tagged: Crenolanib manufacturer, LIF.
Data Availability StatementAll relevant data are within the paper. and a lower population of CD80+ cells. We also evaluated the fungicidal capacity of eosinophils candida cells, although eosinophils of individuals were less responsive to IL-5 activation than settings. Conclusion/Principal findings In conclusion, we suggest that eosinophils might play a role in the sponsor response to fungi and in the pathophysiology of PCM by inducing an intense and systemic inflammatory response in the initial phase of the illness. Author summary Paracoccidioidomycosis (PCM) is definitely a fungal disease endemic of some Latin America countries. The acute clinical form of the disease, which affects children and young adults, is the most severe form of PCM. It is characterized by a stressed out T cell immunity and improved quantity of blood eosinophils that decreases after antifungal treatment. The part of eosinophils in PCM has never been investigated. We found high levels of eosinophil granules and chemokines in serum of individuals. Moreover, individuals eosinophils have a higher migratory and adhesion capacity compared to settings. Our results indicate that eosinophils may participate in the early methods of sponsor response to fungi advertising an intense and systemic inflammatory response, which may result in an inefficient immune response against in vivo. Intro Paracoccidioidomycosis (PCM) is definitely Crenolanib manufacturer a systemic mycosis caused by dimorphic fungi of the genus. Lif It is the most common systemic mycosis of Latin America and, in Brazil, it is the leading cause of death among immunocompetent individuals [1C4]. PCM is definitely caused by inhalation of environment conidia. The fungus may remain latent in cells for years, without any medical manifestation. Depending on the inoculum or sponsor immune response, the disease may develop into two medical forms: the acute/subacute form, which affects young adults and children, or the chronic form, which affects older adults [5]. The acute/subacute or juvenile form comprises 10% of all cases. It is the most severe form of PCM, characterized by diffuse lymph node involvement, hepatosplenomegaly and bone marrow dysfunction. It may also affect pores and skin and bones. Young individuals of both genders are equally affected [3, 6, 7]. Individuals with acute form of PCM have a depressed cellular immune response as evidenced by delayed-type hypersensitivity (DTH) bad tests, deficient lymphocyte proliferation to candida antigens and the production of Th2 cytokines such as IL-4, IL-5, IL-10 and TGF- [8]. In addition, these individuals produce high levels of IgE and IgG4 antibodies against [9]. Also in this form, eosinophilia had been correlated with bad delayed hypersensitivity pores and skin tests, lower CD4 cells quantity and high levels of anti-antibodies, in addition to disease activity and severity [10, 11]. This improved quantity of eosinophils typically results to normal after antifungal treatment [10, 12C14]. However, little is known about the part of these cells in the pathogenesis of PCM. The part of eosinophils in health and disease offers received more attention in the past decades [15C17]. Eosinophils, generally correlated with immune reactions during sensitive and parasitic diseases [18, 19] participate in both innate and adaptive immunity, since it activates and interacts with several immune cells, Crenolanib manufacturer including dendritic cells Crenolanib manufacturer and T lymphocytes [20]. Eosinophils are recruited from your circulation to the inflammatory foci in response to numerous stimuli. Eosinophil degranulation and launch of cytotoxic molecules, i.e. MBP, ECP, EPO and EDN, can quickly impact the microenvironment and influence cell recruitment, tissue repair, homeostasis and remodeling, and also promote a direct response against the pathogen [17, 21]. In addition, eosinophils can present antigen to T lymphocytes and, consequently, act as antigen showing cells (APC) and initiate an immune response to specific antigen [22]. Eosinophils can also act as an effector cell, inducing cells damage and dysfunction, as well as advertising exacerbation of the inflammatory response through the release of toxic proteins using their granules, cytokines and lipid mediators [23, 24]. To day, you will find no studies evaluating the.
Data Availability StatementAll relevant data are within the paper. of CSCs.
Posted in: Main.	Tagged: Bosutinib manufacturer, IRS1.
Data Availability StatementAll relevant data are within the paper. of CSCs. We assessed the impact on ectopically upregulated or downregulated manifestation of HOTAIR in CSCs by smooth agar, self-renewal capacity and CCK-8 assays. The practical website of HOTAIR was determined by truncation. RT-qPCR and semiquantitative Western blotting Bosutinib manufacturer were performed to detect the manifestation levels of genes of interest. Chromatin IP (ChIP) was used to detect the transcriptional regulatory activity of p53 on its target gene. Results After the recognition of CSC properties, RT-qPCR analysis exposed that HOTAIR, but not additional cancer-associated lncRNAs, is definitely highly upregulated in both CSC-MCF7 and CSC-MB231 populations compared with MCF7 and MB231 populations. By modulating the level of HOTAIR manifestation, we showed that HOTAIR tightly regulates the proliferation, colony formation, migration and self-renewal capacity of CSCs. Moreover, full-length HOTAIR transcriptionally inhibits miR-34a specifically, leading to upregulation of Sox2, which is definitely targeted by miR-34a. Ectopic intro of miR-34a mimics reverses the effects of HOTAIR within the physiological processes of CSCs, indicating that HOTAIR affects these processes, including self-renewal capacity; these effects are dependent on the rules of Sox2 via miR-34a. Interestingly, tight transcriptional rules of p53 by HOTAIR was found; accordingly, p21 is definitely indirectly controlled by HOTAIR, resulting in cell cycle access. Summary These results suggest that HOTAIR is definitely a key regulator of proliferation, colony formation, invasion and self-renewal capacity in breast CSCs, which happens in part through rules of Sox2 and p53. Introduction lncRNAs, which are typically non-protein coding transcripts longer than 200 nucleotides, can epigenetically interact with transcription Bosutinib manufacturer factors, transcriptional activators or repressors, and different subunits of complexes, including RNA polymerase (RNAP) II and even duplex DNA, to function as transcriptional or post-transcriptional regulators [1]. As a result of their regulatory tasks, lncRNAs strongly influence the malignant behavior of malignancy, such as tumorigenesis, proliferation, apoptosis, chemoresistance and invasiveness [1]. For example, metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1), an evolutionarily highly conserved and ubiquitously indicated lncRNA, is definitely reportedly highly upregulated in several human being malignancies in addition to lung malignancy, and it was found out to be tightly associated with medical guidelines and advertised invasion and metastasis [2]. However, the functions of thousands of lncRNAs are still unfamiliar, and the degree of their involvement in tumorigenesis is only beginning to become recognized. HOTAIR (Hox transcript antisense intergenic RNA), an approximately 2.2 kb-long non-coding RNA transcribed from your HOXC locus, epigenetically functions like a repressor of HOXD [3]. A novel molecule in the field of tumor biology, HOTAIR has been correlated with metastasis in IRS1 a variety of tumor types, including colorectal [4], pancreatic [5], lung [6] and breast [7] cancers. Notably, a study of its regulatory mechanism in breast tumor exposed that HOTAIR promotes breast tumor metastasis, partly by interacting directly with polycomb repressive complex-2 (PRC2) through its 5 website to induce genome-wide retargeting of PRC2 to hundreds of genes involved in metastasis. The result is definitely H3K27 methylation, which Bosutinib manufacturer epigenetically silences these genes [7]. Moreover, HOTAIR directly inhibits WIF-1 manifestation by advertising H3K27 methylation in the responding promoter region, therefore activating Wnt/-catenin signaling [8]. Accordingly, HOTAIR takes on key tasks in the epigenetic rules of breast cancer malignancy. Breast cancer is one of the most common diseases in females, and several novel therapeutics have been developed thus far. Nonetheless, metastasis remains poorly understood, is largely Bosutinib manufacturer incurable, and is the main cause of cancer-related death [9]. Recently, the malignancy stem-like cell (CSC) hypothesis offers provided new insight into tumorigenesis and metastatic progression, potentially explaining the metastatic mechanisms of breast tumor. This hypothesis suggests that breast CSCs, a subpopulation of breast cancer cells, but not the original tumor cells are responsible for tumor development, metastasis, and transplantation processes [10]. In unique.
Cisplatin, a used anticancer medication broadly, damages locks cells in cochlear
Posted in: Main.	Tagged: Arranon cost, CD61.
Cisplatin, a used anticancer medication broadly, damages locks cells in cochlear organotypic civilizations at low dosages, but paradoxically causes small damage at great doses producing a U-shaped dose-response function. powerful electrophile that reacts with nucleic acids in DNA, leading to intrastrand and interstrand combination linking of DNA resulting in cell routine arrest which blocks tumor proliferation (Cepero et al., 2007). Cisplatin isn't only nephrotoxic, neurotoxic, but also problems the sensory locks cells and neurons in the cochlea and vestibular program. Cisplatin initially problems the cochlear external locks cells (OHC) accompanied by internal locks cells (IHC) and harm spreads in the high-frequency foot of the cochlea to the apex with raising dose or length of time of treatment (Ding et al., 2012a; Fleischman et al., 1975; Saito et al., 1995). In cochlear civilizations, cisplatin damages locks cells through the entire cochlea and also other buildings (Laurell and Bagger-Sjoback, 1991; Meech et al., 1998) including spiral ganglion neurons (SGN) (Alam et al., 2000; Ding et al., 2012a; Gabaizadeh et al., 1997). For toxicity that occurs cisplatin should be transported in to the cytoplasm initial. The pathways that regulate the translocation and uptake of cisplatin into sensory locks cells, neurons, and helping cells in the Arranon cost inner ear are understood poorly. However, recent proof shows that cisplatin uptake is normally mediated by copper transporters that regulate the import, export and sequestration of platinum (Ding et al., 2011b, CD61 2012a, 2013b; Holzer et al., 2004b; Katano et al., 2004; Komatsu et al., 2000; Kuo et al., 2007; Safaei, 2006; Safaei et al., 2008; Howell and Samimi, 2006; Yoshizawa et al., 2007). Copper can be an important metal involved with important biological procedures such as for example oxidative phosphorylation (cytochrome Arranon cost oxidase), catecholamine synthesis, antioxidant defenses (Cu/Zn superoxide dismutase) and iron homeostasis (Puig and Thiele, 2002). Arranon cost Great intracellular concentrations of copper are dangerous (Olivari et al., 2008), cells firmly regulate intracellular copper homeostasis generally through Ctr1 as a result, ATP7A and ATP7B (Holzer et al., 2004a; Katano et al., 2002; Komatsu et al., 2000; Samimi et al., 2004; Lippard and Wang, 2005). Copper uptake through the plasma membrane is normally governed by Ctr1 (Lee et al., 2002) (Lee et al., 2002; Safaei, 2006), which also imports platinum-based substances (Holzer et al., 2004a; Kuo et al., 2007). ATP7A sequesters unwanted platinum or copper, and Arranon cost ATP7B expels these substances in the cytoplasm (He et al., 2011; Kalayda et al., 2008; Kuo et al., 2007; Howell and Safaei, 2005; Samimi et al., 2004). These total outcomes claim that adjustments in Ctr1, ATP7B and ATP7A could modulated the toxic ramifications of cisplatin on vestibular locks cell. To check this hypothesis, we treated postnatal vestibular explants with several doses of cisplatin to see whether high doses of cisplatin had been less dangerous than low doses also Arranon cost to examine cisplatin uptake. 2.?Methods and Materials 2.1. Vestibular body organ cultures Postnatal time 3 Sprague-Dawley rat pups (Charles River, Wilmington, MA) had been used to get ready vestibular body organ cultures regarding to procedures specified in detail inside our prior magazines (Ding et al., 2012b, 2013a; Dong et al., 2014). Quickly, rat pups had been decapitated as well as the macula from the utricle, excellent and lateral crista ampullae had been micro-dissected out combined with the macula of saccule and posterior crista ampulla. The otolithic membranes over the macula of utricle and saccule had been removed and the vestibular explants had been positioned on the collagen gel on underneath of the 30?mm culture enough and dish serum-free moderate put into promote the attachment from the explant towards the gel. The culture dish was put into an incubator at 37 then?C in 5% CO2 for 1?h. Soon after, 0.7?ml of serum-free moderate was put into cover the explants. The civilizations had been put into an incubator and preserved at 37?C in 5% CO2 right away. On the next time, the vestibular explants had been treated with 0, 10, 50, 100, 400 or1000?M cisplatin in lifestyle moderate for 48?h..
Supplementary MaterialsIDRD_Zhang_et_al_Supplemental_Content material. control groups but not L02 cells. However, the
Posted in: Main.	Tagged: HTRA3, SNS-032 manufacturer.
Supplementary MaterialsIDRD_Zhang_et_al_Supplemental_Content material. control groups but not L02 cells. However, the cytotoxicity HTRA3 of GPCC was lower than that of free CPT, which could become explained from the slower launch of CPT from your GPCC compared with free CPT. Additional tumor targeting experiments demonstrated the superior tumor-targeting ability of the GPCC conjugate, which significantly accumulated in tumor in the mean time minimize in normal SNS-032 manufacturer cells compared with control organizations. The GPCC conjugate showed better pharmacokinetic properties, enabling a prolonged blood circulation time and improved camptothecin area under the curve (AUC). These features contributed to better restorative effectiveness and lower toxicity in H22 hepatocarcinoma tumor-bearing mice. The GLUT1-focusing on, GSH-sensitive GPCC conjugate provides an efficient, safe and economic approach for tumor cell targeted drug delivery. 1.01 (t, 3H, CCH2CH3), 2.19C2.25 (m, 2H, CCH2CH3), 2.65 (t, 2H, CCH2SSCH2C), 2.88 (t, 2H, CCH2SSCH2C), 2.94C2.97 (m, 4H, COCCH2CH2SSCH2CH2COOH), 5.31 (s, 2H, CCOOCH2C), 5.40C5.43 (d, 1H, CNCH2CC), 5.62C5.65 (d, 1H, CNCH2CC), 7.58 (s, 1H, C(CH2)(CO)NC(C?=?N)=CHC), 7.69 (t, 1H, (C6H4)CH?=?C(CH2)(C?=?N)C), 7.85, 8.00, 8.20, 8.55 (t, d, d, s, 1H, 1H, 1H, 1H, phenyl). ESI-QTRAP-MS: determined from C26H24N2O7S2 C H?: 539.10249; observed: 539.09412. Synthesis of PAMAMCCPT conjugate CPT derivative (188.69?mg, 0.35?mmol) dissolved in DMSO was added to DMAP (43?mg, 0.35?mmol), EDC (67?mg, 0.35?mmol), and NHS (67?mg, 0.35?mmol) to synthesize the CPT active ester. The combination was reacted for 24?h at space temperature and used directly without further purification. PAMAM (248.76?mg, 1.75??10?2?mmol) was added, and the combination was reacted for another 24?h. The free CPT derivative was eliminated by dialysis against water for 24?h; the water was refreshed every 8?h. The final product, PAMAMCCPT (Personal computer), was concentrated with ultrafiltration tube, lyophilized and characterized by 1HNMR and UVCVis spectroscopy. Synthesis of PAMAMCCPTCCy7 conjugate Personal computer (100?mg, 5.94??10?3?mmol) and Cy7-NHS (21.29?mg, 2.97??10?2?mmol) SNS-032 manufacturer were dissolved in PBS (pH 8.0) and vigorously agitated for 24?h in dark (Ma et?al., 2017). Free Cy7-NHS was eliminated by dialysis against SNS-032 manufacturer water for 24?h, and the residue was concentrated, lyophilized and characterized by 1HNMR and UVCVis spectroscopy. Synthesis of mPEG/glucoseCPEGCPAMAMCCPTCCy7 conjugate PAMAMCCPTCCy7 (PCC, 100?mg, 5.20??10?3?mmol) was dissolved in PBS (pH 8.0); glucose-PEG-NHS (1300.03?mg, 0.26?mmol) was added, and the combination was reacted for 24?h at space temperature. The reaction combination was purified to remove free PEG by dialysis against water for 24?h, and the final product, GlucoseCPEGCPAMAMCCPTCCy7 (GPCC), was concentrated, lyophilized, and characterized by 1HNMR and UVCVis spectroscopy. The mPEG-NHS was also reacted with PCC to yield an mPEGCPAMAMCCPTCCy7 (MPCC) conjugate as control (He et?al., 2011). Characterization of conjugates Size, zeta potential, and morphology The particle sizes and zeta potential of different conjugates at 10?mg/ml were determined using a dynamic light scattering (DLS) particle size analyzer (Nicomp 380 Zeta Potential/Particle Sizer, Santa Barbara, CA). Following staining with 2% sodium phosphotungstate remedy, the conjugate morphologies were observed using transmission electron microscopy (TEM) at an accelerating voltage of 100?kV (JEM 1400 JOEL, Akishima City, Tokyo Prefecture, Japan). In vitro drug release A dialysis method was used to determine the CPT launch of conjugates. Conjugates at concentrations of 1 1?mg/ml were placed in dialysis hand bags (MWCO SNS-032 manufacturer 3500?Da) and immersed in 50?ml PBS (pH 7.4) containing different concentrations of GSH (0?M, 10?M and 10?mM) to mimic various cellular microenvironment conditions. The drug launch was carried out at 100?rpm and 37?C. During dialysis, 1?ml aliquots were withdrawn from your launch medium at predefined intervals, and 1?ml of fresh launch medium was added to maintain a constant total volume. The CPT concentrations were determined by HPLC. cell focusing on evaluation HepG2 and L02 cells were seeded into 96-well tradition plates at a denseness of 1 1.2??104 cells/well and incubated for 24?h. After the confluency and morphology were checked, 1?M conjugates (calculated from CPT) were added to each well and co-incubated with the cells for different times (1, 4, and 8?h). To confirm the specificity of the GPCC conjugate, another group of HepG2 cells was preincubated with 2.5?mM d-glucose (d-GLU) for 4?h to block the GLUT1 transporter. The D-GLU was a substrate as well as inhibitor of GLUT1. Then, the GPCC conjugate was co-incubated with the cells for 4?h. At the end of the incubation time, the conjugate solutions were withdrawn from your wells, and the cells were washed three times with chilly PBS. After fixation with 4% paraformaldehyde, the cells were qualitatively analyzed by fluorescence microscopy (IX71, Olympus, Tokyo, Japan). After trypsinization and re-suspension of the cells in PBS (pH 7.4), the fluorescence intensity was quantitatively analyzed by circulation cytometry (BD FACSAria III, Piscataway, NJ). Besides that, a multicellular tumor spheroids (MCTS) model, which was more suitable for mimicking the tumor microenvionment, was also used like a product to monolayer cell model for better estimating focusing on effectiveness (Kunz-Schughart, 1999). MCTS model.


text:
Florence, Ala. Hit By Ransomware 12 Days After Being Alerted by KrebsOnSecurity
In late May, KrebsOnSecurity alerted numerous officials in Florence, Ala. that their information technology systems had been infiltrated by hackers who specialize in deploying ransomware. Nevertheless, on Friday, June 5, the intruders sprang their attack, deploying ransomware and demanding nearly $300,000 worth of bitcoin. City officials now say they plan to pay the ransom demand, in hopes of keeping the personal data of their citizens off of the Internet.
Nestled in the northwest corner of Alabama, Florence is home to roughly 40,000 residents. It is part of a quad-city metropolitan area perhaps best known for the Muscle Shoals Sound Studio that recorded the dulcet tones of many big-name music acts in the 1960s and 70s.
Image: Florenceal.org
On May 26, acting on a tip from Milwaukee, Wisc.-based cybersecurity firm Hold Security, KrebsOnSecurity contacted the office of Florence's mayor to alert them that a Windows 10 system in their IT environment had been commandeered by a ransomware gang.
Comparing the information shared by Hold Security dark web specialist Yuliana Bellini with the employee directory on the Florence website indicated the username for the computer that attackers had used to gain a foothold in the network on May 6 belonged to the city's manager of information systems.
My call was transferred to no fewer than three different people, none of whom seemed eager to act on the information. Eventually, I was routed to the non-emergency line for the Florence police department. When that call went straight to voicemail, I left a message and called the city's emergency response team.
That last effort prompted a gracious return call the following day from a system administrator for the city, who thanked me for the heads up and said he and his colleagues had isolated the computer and Windows network account Hold Security flagged as hacked.
"I can't tell you how grateful we are that you helped us dodge this bullet," the technician said in a voicemail message for this author. "We got everything taken care of now, and some different protocols are in place. Hopefully we won't have another near scare like we did, and hopefully we won't have to talk to each other again."
But on Friday, Florence Mayor Steve Holt confirmed that a cyberattack had shut down the city's email system. Holt told local news outlets at the time there wasn't any indication that ransomware was involved.
However, in an interview with KrebsOnSecurity Tuesday, Holt acknowledged the city was being extorted by DoppelPaymer, a ransomware gang with a reputation for negotiating some of the highest extortion payments across dozens of known ransomware families.
The average ransomware payment by ransomware strain. Source: Chainalysis.
Holt said the same gang appears to have simultaneously compromised networks belonging to four other victims within an hour of Florence, including another municipality that he declined to name. Holt said the extortionists initially demanded 39 bitcoin (~USD $378,000), but that an outside security firm hired by the city had negotiated the price down to 30 bitcoin (~USD $291,000).
Like many other cybercrime gangs operating these days, DoppelPaymer will steal reams of data from victims prior to launching the ransomware, and then threaten to publish or sell the data unless a ransom demand is paid.
Holt told KrebsOnSecurity the city can't afford to see its citizens' personal and financial data jeopardized by not paying.
"Do they have our stuff? We don't know, but that's the roll of the dice," Holt said.
Steve Price, the Florence IT manager whose Microsoft Windows credentials were stolen on May 6 by a DHL-themed phishing attack and used to further compromise the city's network, explained that following my notification on May 26 the city immediately took a number of preventative measures to stave off a potential ransomware incident. Price said that when the ransomware hit, they were in the middle of trying to get city leaders to approve funds for a more thorough investigation and remediation.
"We were trying to get another [cybersecurity] response company involved, and that's what we were trying to get through the city council on Friday when we got hit," Price said. "We feel like we can build our network back, but we can't undo things if peoples' personal information is released."
A DoppelPaymer ransom note. Image: Crowdstrike.
Fabian Wosar, chief technology officer at Emsisoft, said organizations need to understand that the only step which guarantees a malware infestation won't turn into a full-on ransomware attack is completely rebuilding the compromised network — including email systems.
"There is a misguided belief that if you were compromised you can get away with anything but a complete rebuild of the affected networks and infrastructure," Wosar said, noting that it's not uncommon for threat actors to maintain control even as a ransomware victim organization is restoring their systems from backups.
"They often even demonstrate that they still 'own' the network by publishing screenshots of messages talking about the incident," Wosar said.
Hold Security founder Alex Holden said Florence's situation is all too common, and that very often ransomware purveyors are inside a victim's network for weeks or months before launching their malware.
"We often get glimpses of the bad guys beginning their assaults against computer networks and we do our best to let the victims know about the attack," Holden said. "Since we can't see every aspect of the attack we advise victims to conduct a full investigation of the events, based on the evidence collected. But when we deal with sensitive situations like ransomware, timing and precision are critical. If the victim will listen and seek out expert opinions, they have a great chance of successfully stopping the breach before it turns into ransom."
Tags: alex holden, CrowdStrike, DoppelPaymer, Emsisoft, Fabian Wosar, Hold Security, Steve Price, Yuliana Bellini
This entry was posted on Tuesday, June 9th, 2020 at 1:05 pm	and is filed under Latest Warnings, Ransomware. You can follow any comments to this entry through the RSS 2.0 feed. Both comments and pings are currently closed.
clockedin employee retention
Aw, this was an incredibly nice post. Finding the timme and actual effort to create a
superb article… buut what can I say… I put things offf a
whole lot and never manage too get nearlyy anything done.
Paul Mikol
Cyberhero Krebs I'll say it once again:
You are indeed the man…
PolDisp
The city I work for has monthly computer training about events like this. You know training on how to hover over links before you click, etc.
Then they blast everyone with HUNDREDS of emails a week including "what's happening" emails in the city that don't affect 90% 0f the employees.
It is only a matter of time before the fatigue of clicking on all those emails will put my city in the same spot. How can you be discerning and inspect every email when you have 2, 3, 4 dozen emails to get through.
John Navas
1. Block links in email.
2. Block attachments to email.
HTML and MIME are security nightmares.
Brian Fiori (AKA The Dean)
Well, good luck doing business with NO links or attachments.
Any reasonably competent IT person should be able to find out if the link/attachment is legit and safe.
It's not about competence, it is about cost.
Nobody is going to have human beings look at every email. There are many Email Security solutions out there. All the good ones cost money.
Probably less than $300,000…
And local/state governments have a very hard time justifying even that cost.
Even many private companies don't want to spend the money on a security solution when they don't understand how it directly makes or saves them money. Cybersecurity spending a big discussion, and you need a good Risk Officer to work out the numbers regarding loss and probability.
Many companies, and governments, simply don't bother, and just purchase insurance. It's good to have insurance, but many places use that as cost/benefit reasoning to NOT buy security solutions.
Rex Easly
Sad thing is, email is horribly insecure and is also probably the most relied on system in most companies. Companies spend all their time trying to make it more safe but it is like trying to make tight-rope walking safe.
Hovering over links is good practice for emails, as the email client is handling the links.
However, be careful using this trick on websites that are less than trustworthy. Websites run javascript, and javascript can be used to dynamically change the URL link as you click on it… so hovering might tell you one think, but clicking on it takes you somewhere else.
You'd have to inspect the DOM to see it… look for functions like "onmousedown", "onclick", etc.
Google does this with their search results too. If they let users click on links and go directly to those websites, they can't collect analytics on what you clicked on. So they fake/spoof their links when you hover over them to look like you'll go straight there. But they run a javascript function "onmousedown" to dynamically change the link to a google controlled URL that would track and then redirect your browser.
Try it for yourself. Do a Google search, hover over the link for one of the results… then right-click that link. Hover over it again, and it'll be a Google URL.
Google just uses it for tracking, but any website can do this for any purpose.
If a ransomware target does nightly backups and keeps seven days of backups, would that work for restoring after a DoppelPaymer attack?
No. As this article demonstrates the network was compromised for months before the ransomeware took effect. probably copied lots O' data.
Yes, for restoration, this will work since IT would just need to know about the ransom within a week of when it starts to encrypt.
BadDNA
Note that the ransom isn't to restore a system. That should be childs play with proper backups, and is only a convenience item to get back. The ransom is to avoid the release of personal information that the public would get their nickers in a twist over. The same public that will pony up the tax payments to make up for the ransom.
Do not confuse "ransomWARE" with ransom demands for data breaches.
Ransomware is malicious software that encrypts the data to deny its access from its rightful owners. That is what the ransom is for.
Most large ransomware campaigns use this denial of data as their leverage for ransom. They may tack on a premium for data that is sensitive or contains PII, as something else to demand. But it hasn't been the primary ransom demand.
Now, with GDPR, CCPA and other privacy regulations, the penalty for breach is much higher. So some attackers may try to extort payment from companies thinking they may pay up because they don't want to be fined.
But this doesn't really work in practice, as it is criminal to not disclose the breach just because the hackers were paid and promised not to release the data to the public. The companies have to pay the data breach fines anyway.
Further, there are no guarantees that hackers won't keep a copy and re-extort later. Companies know this, and rarely give into ransom demands for keeping private data out of the public domain.
In contrast, ransomware hackers DO make guarantees and build up reputations for decrypting data. And companies (and governments) take notice, and do wind up paying these ransoms.
They probably have some Boss Hogg types in there that figure if your call couldn't help them get money or re-elected, there was no need to pay it any mind.
Florence Native
Bingo. I live in Florence and you pegged it.
Dougie Fresh
The state government agency I worked for a year ago had an IT Vice President who would send around emails explaining security basics. Don't click links was a common suggestion. The emails from IT VP would also have further reading and an ingenious security device, a link that says: "click this link, it is safe."
Kim in Minnesota
Well. Should you ever decide to relocate, Mr. Krebs, the Land of 10000 Lakes would welcome you with open arms.
Our Senate website was hit a few weeks ago. Parts of it are still down. Not much information has been offered to the public, but I suspect the breach took place via a third party , (i.e. Zoom, FB, or others), and that my tech savvy congressmen (Kiffmeyer, Lucero, Nash, et al.) are having trouble auditing the trail.
Thank you, as always, for doing what you do.
So they are gonna pay and trust that their extortioners won't sell/release the data anyway? Or demand more $ after payment?
TheVee
IMHO, the mayor plus ought to be replaced. Incompetence reeks with peepz who have no insight and think IT Security is a drain must be held accountable..
SteveP
We've seen a huge increase in phishing attempts the last 2-3 months. Anything with an attachment from outside a few trusted domains with correct SPF get dropped into spam. We train everyone to be fearful of all spam.
if the FROM and REPLY don't match – spam.
We show our people how to setup their email clients to show only ASCii which is mandatory by the SMTP standards. Empty ASCii parts are spam.
Unexpected attachments are quarantined by the system.
Email is one of the systems i love to hate, but we'd never outsource it because we like our clients to have privacy, not feed some huge privacy-sucking marketing company with even more company+personal data.
Is this why I have no data on my phone anymore. My Verizon data had been gone for a week now and I've talked to 8 different people at at Verizon trying to get my data restored but it's just gone. I have to use Wifi now! I'm suppose to have unlimited data……was their a cyber hack as well?
Dylan Rofe
wow, omg..


WCF provides out of home care to children and young people referred to us by FACS. We cater for sibling groups of children on our farming property (120 acres) at Nowra Hill on the South Coast of NSW.
WCF also provide supervised transport, supervised contact, mentoring and crisis care under Southern Brokerage Services.
WCF have a unique "special assistance" school, William Campbell College, currently catering for K-6 (plans for Y7-12) for children in out of home care and other children who may be disadvantaged in the community.


Create spectacular environments with glass tiles. Walls in kitchen, bathroom or why not please. Make the room bigger and more luxurious. Stylish Antiqued Glass Beads are "pretty" vintage. Impossible to capture image.


text:
Seychelles News
Lebanon's Prime Minister Gave $16 Million to South African Model
Saad Hariri did not make the payments while he was prime minister, South African court records show, but the revelation is likely to tarnish his image.
How Much Do You Know About Seychelles?
Can you find Seychelles on a map? What else do you know about this island nation with about 95,000 people?
Seychelles President Makes Underwater Plea to Protect World's Seas
Danny Faure, the leader of the Indian Ocean island nation, spoke from a submersible craft that had dived to 406 feet as part of a scientific research mission.
Billionaire Backer of Maria Butina Had Russian Security Ties
Konstantin Nikolaev, the oligarch who helped finance Ms. Butina, has been an investor in projects linked to the Russian government.
Ancient Crocodiles Once Feasted on Giant Tortoises on This Island
Bite marks on fossils found on an atoll near eastern Africa suggest that this paradise for the world's most common giant tortoise may have once been a much scarier place.
Island Nations, With No Time to Lose, Take Climate Response Into Their Own Hands
Island states that are least responsible for global emissions but most vulnerable to storms and rising seas are looking outside the United Nations process for aid.
Seychelles Reports a Case of Plague
A man fell ill after returning from Madagascar, which is struggling to contain a rash of plague cases. It is the first instance of the outbreak spreading to another country.
Four Resorts for a Private Island Vacation
Properties in Africa and Indonesia offer snorkeling, swimming, sandy beaches and seclusion.
Sven Lindblad Takes Passengers to the Wild Places
The head of Lindblad Expeditions on finding rare natural wonders by ship and promoting conservation consciousness.
Cable and Wireless to Buy Latin American Telecommunications Provider
The deal for Columbus International, a privately held company based in Barbados, is valued at $1.85 billion, including the assumption of debt.
Seychelles at Wikipedia
Seychelles at CIA FactBook


Whether you are looking for your first professional international job after graduating with a master's degree, or you are applying for your first internship or volunteer position abroad you should be aware that international resumes are different from domestic resumes.... How to build a resume. To build a resume in USAJOBS: Sign into USAJOBS. Go to your Documents. Make sure you're in the Resumes section and select the Upload or build resume button.
Build the perfect resume and get the job you want! Build a Resume Go to Dashboard. Start your resume. Traditional. Blue Skies. van Deco. more templates Customize a sample resume. Sales. Customer Service. Nursing. more samples Build a resume in 3 steps... Resumes between 600 and 700 words in length were rated much higher than resumes that were less that 500 words long, and anything over 700 words began to trend towards lower Star Ratings. Keep your resume in the 600-700 word Goldilocks length (not too long, not too short).
How to build a resume. To build a resume in USAJOBS: Sign into USAJOBS. Go to your Documents. Make sure you're in the Resumes section and select the Upload or build resume button.


In case you haven't noticed, my wonderful state of Wisconsin is in a bit of turmoil.
I'm being rather facetious here, because unless you've been vacationing on Mars since February 11, you've certainly witnessed much more than you want of Gov. Scott Walker's proposed budget repair bill. But no matter from what side one views this standoff-watched-round-the-world, everyone agrees it's become one of great passion.
It's also of historic proportion.
Each week as I photograph protesters, I can't help thinking of the images we see in history books—images of early labor unionists protesting for safe working conditions, women suffragettes marching for rights to vote and civil rights activists standing up for human equality. In all those photos, people visually communicated their message with signs.
Isn't it blessed that we live in a country where we have the freedom to do so?
Here's a small sampling of the collective communication going on in Wisconsin (I have to admit, in spite of my journalist's education, my photos are a bit one sided. I was unable to be in Madison the day Walker supporters marched).


I was so pleased to be invited to join and accept a role on the Board of Directors for Instruments of Hope. As a child, I remember attending church with my grand parents. On one particular warm Sunday morning, the stained glass windows of our church building were open. During a silent prayer I heard a pinecone drop from a tree and hit the ground outside. I instantly realized the role of nature in religion, faith and Christianity. In my 6-year old mind I viewed the relationship, as "God is Nature." While many of my beliefs have been challenged and modified during my life I still hold this same tenant as a very important aspect of my personal philosophy.
To this point, I graduated with a liberal science degree in Biology from Lyman Briggs College at Michigan State University. I later earned a Masters degree in Counseling. During this 2nd career I focused on Career Counseling with a specialty in the sciences. I have also been involved with Personal Counseling. I have referred many clients to the Sculpture Garden to have a safe, quiet, peaceful, reflective and meditative space to refresh themselves and commune with nature.
I pledge to continue assisting with the growth and sustainability of the St. Francis of Assisi Sculpture Garden and Meditation Walk. I hope, to that end I am able to meet you, share stories and the beauty of the garden.


It is cold outside here in the Midwest, and most everywhere else it seems. That makes it a great time to stay home and cook up some wonderful soups and stews on your cooktop stove. Our family favorite is beefy vegetable. Invariably when I am browning the hamburger I splatter at least a little grease on my cooktop. And, of course, as I am stirring and tasting the soup a few drops always end up spilling onto my stovetop. I always use Cerama Bryte Cooktop Cleaner to clean up all the spills and spatters to keep my cooktop stove spotless. If you have any extra-tough spills or burnt-on food, the Cerama Bryte Scraper is your go-to tool. Check out our "How-To Videos" on our home page at www.ceramabryte.com for instructions on how to clean your stove and how to safely use the Cerama Bryte Scraper on your cooktop. Stay warm, and remember spring isn't too far away!