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Posted by admin in June, PremiumContent
Friday, June 17, 2016 – Edition: #5755
Get a Load of This Sheet!
★ Today the private funeral for Christina Grimmie is being held. The 22-year-old singer, who appeared on "The Voice" in 2014, was killed last week when 27-year-old gunman Kevin James Loibl opened fire during a meet-and-greet after her concert in Orlando FL. Her family, including brother Marcus who tackled Loibl after he shot Christina, pays final respects tonight at a small, intimate memorial service in her hometown of Medford NJ.
– People.com
★ James Corden is homesick. The 37-year-old Brit actor-comedian and his family moved to the US in 2015 after he landed one of the most sought-after jobs in show biz … "The Late Late Show". But a year later an insider say he's considering a return to the UK as soon as his $14-million deal with CBS-TV expires. the source claims Corden is keen to do more work in theater and movies. But a move back home isn't likely any time soon – he's contracted to host the late night show until 2020. Even Corden admits: "They can still fire me, but I can't leave."
– Mail Online
★ And 70-year-old actress Helen Mirren is joining Vin Diesel and Dwayne Johnson in "Fast 8", the next film in the "Fast & Furious" franchise, to be directed by "Straight Outta Compton" filmmaker F Gary Gray. Also in the cast are Charlize Theron, Scott Eastwood, and Kristofer Hivju from "Game of Thrones". Mirren's role is as yet undisclosed, but she says she's hoping she gets to do some driving. She donned a racing helmet for an episode of the Brit car show "Top Gear" back in 2007. "Fast 8" is scheduled to hit theaters in April 2017.
– "Elle Magazine"
WEEKEND SHOW BIZ SKED:
• "Ellen DeGeneres Show" (syndicated/CTV2) – Today Dan + Shay ("Obsessed").
• "Good Morning America" (ABC) – This morning Demi Lovato ("Confident").
• "Last Call With Carson Daly" (NBC) – Tonight Moby ("Long Ambients 1: Calm. Sleep.). Rerun.
• "The Last Ship" (TNT) – Sunday the 2-hour Season 3 premiere, delayed a week because the storyline features attackers shooting up a crowded nightclub. Too close to last week's real-life tragedy in Orlando FL.
• "Late Show With Stephen Colbert" (CBS/Global) – Tonight Silversun Pickups ("Better Nature").
• MuchMusic Video Awards (MuchMusic) – Sunday the annual block party in Toronto ON is hosted by Gigi Hadid. Alessia Cara and Drake lead nominees with 7 nominations apiece, followed by The Weeknd and Shawn Mendes with 6 each. Other multiple nominees include Adele, Taylor Swift, and ZAYN. Performers include Fifth Harmony, Hailee Steinfeld, Macklemore & Ryan Lewis, Nick Jonas, and Tegan & Sara.
NET: http://mmva.muchmusic.com
• "Orange Is the New Black" (Netflix) – Today Taylor Schilling and her prison mates are back with the 4th season release.
• "Today Show" (NBC) – This morning Charlie Puth ("Nine Track Mind").
• David Bowie – Saturday at the Music Hall in Detroit MI, Complexions Contemporary Ballet opens a new production featuring dance interpretations of his music. It's not the only Bowie tribute in the city; Detroit Symphony is performing "The Music of David Bowie" July 10th.
• Garth Brooks – This weekend he returns to Winnipeg MB for the first time in 20 years. "The Garth Brooks World Tour with Trisha Yearwood" plays 4 shows over 3 nights.
• Maxwell – Tonight at 10pm ET, he's rehearsing for his new tour live on Facebook. The 25-date Summer tour includes a headlining slot at New Orleans' Essence Festival in early July.
• Mumford & Sons – Today they release "Johannesburg", a mini-album recorded in Studio 2 and the Auditorium of the South African Broadcasting Corporation in February over 2 all-day and all-night sessions.
• Jon Pardi – Today the country singer releases his 2nd full album,"California Sunrise", featuring the lead single "Head Over Boots".
• Radiohead – Their 9th studio album, "A Moon Shaped Pool", has a physical release today on CD and vinyl. The band is staging an all-day event in record stores worldwide called "Live from a Moon Shaped Pool" that features a day-long audio stream, contests, and more.
NET: http://www.amoonshapedpool.com/livefrom
• Rascal Flatts – Tonight they hit the road on the "Rascal Flatts: Rhythm & Roots Tour", beginning in Charlotte NC. Opening acts are Kelsea Ballerini and Chris Lane.
• Red Hot Chili Peppers – Today they release the new album, "The Getaway". It's their first new album since 2011's "I'm With You".
• Taylor Swift – Is 'Hiddleswift' the next big celeb couple? Just weeks after she split with Calvin Harris, she's been pictured looking distinctly close to 35-year-old Brit actor Tom Hiddleston on the beach near her Rhode Island mansion. Reports claim they're "in a secret romance".
• The Who – Summertime booze? Saturday frontman Roger Daltrey launches a new Champagne to honor the band's 50th anniversary. A percentage of the proceeds from limited-edition Champagne Cuvée Roger Daltrey benefits Teen Cancer America.
TODAY'S MOVIE OPENINGS:
• "Central Intelligence" ( PG-13 Action Comedy ): After he reunites with an old high school pal through Facebook, a mild-mannered accountant is lured into the world of international espionage. The first movie pairing of Dwayne Johnson and Kevin Hart. Co-stars Aaron Paul, Amy Ryan, and Danielle Nicolet.
NET: http://www.centralintelligencemovie.com
• "Finding Dory" ( PG Animated Adventure ) – In the sequel to "Finding Nemo" (2003), 'Dory' (Ellen DeGeneres) goes in search of her family whom she had forgotten. Eventually the friendly-but-forgetful blue tang fish reunites with her loved ones and everyone learns a few things about the real meaning of family. Voice cast also includes Albert Brooks, Ed O'Neill.
NET: http://movies.disney.com/finding-dory
• Opening in limited release: "Clown" (Horror); "The Last King" (Action Adventure); "Raiders!: The Story Of the Greatest Fan Film Ever Made" (Documentary); "Swiss Army Man" (Adventure Comedy); and "Tickled" (Documentary).
WHAT YOUR UNDERWEAR SAYS ABOUT YOU:
Feeling a little down in the dumps? Maybe you just need to change your underwear. Seriously. Anjel OBryant is Australia's leading color-response analyst, who specializes in 'scientifically based color response technology', according to her website. She claims the color of your undies can directly affect your mood. Quote: "Every color vibrates, all at different levels. It doesn't matter if you can see them or not, it goes through your skin, over your whole body and into your brain." Right. She recommends red underwear because it gives you the energy and boost to power through long hours at work. Other primary colors also are said to provide positive energy. Black, white or grey undies, on the other hand, either have negative energy or don't 'vibrate' at all. (Forget about the hue, we're a bit unsure about this 'vibrating' underwear.)
NET: http://tinyurl.com/hcl5282 (scroll down for what colors mean)
– "Daily Mail"
Nearly two-thirds of shoppers who've bought women's clothes in the last 6 months have sent or taken at least 1 item back.
– BBC News
1963 [53] Greg Kinnear, Logansport IN, movie actor ("Anchorman: The Legend Continues", "Little Miss Sunshine")
1980 [36] Venus Williams, Lynwood CA, former #1-ranked women's tennis player (now #9) with fastest recorded serve in WTA history (127 mph)/older sister of tennis star Serena Williams
1987 [29] Kendrick Lamar, Compton CA, rapper-songwriter (f/Drake -"Poetic Justice", "Swimming Pools [Drank]")
SATURDAY –
Billionaire musician Paul McCartney (The Beatles) is 74; Rock keyboardist Dizzy Reed (Guns N' Roses) is 53; Oldies singer Nathan Morris (Boyz II Men) is 45; Singer-songwriter Ray LaMontagne ("Trouble") is 43; Country singer Blake Shelton ("Came Here to Forget") is 40; Indie rock guitarist Steven Chen (Airborne Toxic Event) is 38; TV actor Jacob Anderson ("Game of Thrones") is 26.
SUNDAY –
Singer-dancer Paula Abdul ("So You Think You Can Dance?") is 54; TV personality Lara Spencer ("Good Morning America") is 47; Rock guitarist Brian 'Head' Welch (KoRn) is 46; folk-rock singer-banjoist Scott Avett (Avett Bros) is 40; Movie actress Zoe Saldana ("Avatar") is 38; Rapper-singer Macklemore (Macklemore & Ryan Lewis) is 33.
TODAY'S BS REASONS TO PARTY . . .
• "Avon Heritage Duct Tape Festival", the 13th annual in Avon OH, home to the manufacturer of Duck Brand Duct Tape. Included in the festivities is a 'Duct Tape Fashion Show'.
NET: http://www.avonducktapefestival.com
• "Canadian North Midnight Classic", the 68th annual all-night golf tournament in Yellowknife, Northwest Territories, possible because there's 24 hours of daylight at the Summer Solstice.
NET: https://yellowknifegolf.com/news/tournaments
• "Eat Your Vegetables Day", an observance likely begun by somebody's mommy somewhere.
• "Flip-Flops Day", an annual excuse to expose those toes. People have been wearing flip-flops for millennia, going back to the Ancient Egyptians in 4000 BC.
• "Work@Home Father's Day", held the Friday before "Father's Day" to honor fathers 'who work from home as a means of providing balance between work and family interaction.
• "World Day to Combat Desertification & Drought", hifalutin UN terminology suggesting we may have a water shortage problem in many parts of the world.
• "Autistic Pride Day", an annual celebration of the 'neuro-diversity' of people on the autism spectrum that's designed to shift views of autism from 'disease' to 'difference'.
• "International Picnic Day" a great time to enjoy a meal 'al fresco', or if you don't know him … anyone else you choose. It's a pseudo-holiday for humans but a regular working day for ants.
• "International Sushi Day", the 7th annual observance conceived originally as a Facebook page. If you're looking for an excuse to eat raw fish, this seems as good a day as any.
NET: http://bit.ly/10Xizp
• "Splurge Day", when we're encouraged to go out and do something indulgent. If funds or guilt were no problem, what would you blow bucks on?
• "World Juggling Day", observed annually on the Saturday closest to June 17th, created by the International Jugglers Association to spread the fun of juggling.
• "Fathers Day", celebrated on the 3rd Sunday in June in about 20 countries worldwide as a day to honor Dad and appreciate all he does.
• "Martini Day". Recipe for a very dry martini: Take 1 bottle of gin …
NET: http://www.punchbowl.com/holidays/national-martini-day
• "World Sauntering Day", a day to 'revive the art of Victorian sauntering, and discourage jogging, lollygagging, sashaying, fast walking, and trotting'. It's "Martini Day", can we stumble?
2005 [11] Coldplay's "X&Y" album achieves #1 status in 22 countries worldwide
2006 [10] Rolling Stones' Ronnie Wood enters rehab to kick his drinking habit (he recovers in time to join the band on its latest world tour a month later)
TODAY'S FIRST . . .
2011 [05] 1st condemnation of discrimination against gays, lesbians, and transgender people is issued by the United Nations Human Rights Council
TODAY'S RECORD . . .
2012 [03] Bruce Springsteen plays his longest concert – a 3-hour, 48-minute, 32-song set at the Estadio Santiago Bernabeu in Madrid, Spain
COMING UP . . .
[Mon] Summer begins
[Mon] Motorcycle to Work Day
[Mon] World Productivity Day
[Mon] Full 'Strawberry' Moon
[Tues] Selfie Day
[Tues] Tall Girl Appreciation Day
This Week Is … Universal Fathers Week
This Month Is … Camping Month
HONEST FATHERS DAY GREETINGS:
• "I don't know what your favorite sport is, but I hope you enjoy it all day: Happy Father's Day!"
• "I prefer texting to phone calls: Happy Father's Day!"
• "You are worth so much more to me than this e-card: Happy Father's Day!"
• "I think your hair is really, really dated: Happy Father's Day!"
• "No, I haven't read that book you gave me 5 years ago yet: Happy Father's Day!"
• "Sometimes it's hard to believe we're related: Happy Father's Day!"
• "This card cost $5 so it's technically also your gift: Happy Father's Day!"
• "I'm sending this card because I don't want to call you: Happy Father's Day!"
• "This all feels a bit forced: Happy Father's Day!"
• "See you at Christmas, maybe: Happy Father's Day!"
– Thanks to Cetoria Tomberlin
What do you call a guy who asks a hundred girls out before getting a date? A guy with a date.
☎ If you could only eat one thing for the rest of your life, what would it be?
Question: Having THIS increases your chances of landing a job by 10%.
Answer: White teeth.
Act like you know what you're doing in every situation and 99% of people will leave you alone.


Full and darkly-shaded brows continued to wow at Dolce & Gabbana, Burberry, Oscar de la Renta and Alexander Wang this year. It's quite the transformation so it might take you a while to get used to. Once you see how this look frames your face, you won't want to go out without it. Tip: if you're going to sport this look, we suggest minimal make-up elsewhere.


It is not just the design and build of our garden rooms that are produced with sustainability and the sympathy of the environment at heart. There are other reasons why deciding to build a garden room can help the environment, the most obvious and impactful being the decision to make the lifestyle change of bringing your office to your home. If you use your contemporary garden studios as an office, then there is no commute to work, saving a considerable amount of energy, not to mention time, money and stress!
Without boring you with the science too much, here's how we approach sourcing materials and designing and building our garden rooms so that they work in synergy with the environment. We have approached our design process with sustainability and design aesthetics running in parallel. We all want to help sustain the environment where we can but equally we want a build that is contemporary and pleasing to the eye and it will be with our designer garden homes. In.it.studios feel our end product demonstrates what sustainable architecture can achieve without compromising the aesthetics or increased costs which often reduce the eco credentials of a new build.
The timber for our garden rooms is sustainably sourced timber and is used throughout the whole structure. The environmental impact of using this wood is that the forests where the timber has typically been sourced, is well managed and the trees are replanted to sustain the harvesting of this natural product. Very little energy is required in sourcing timber in comparison to the energy involved in producing bricks or concrete blocks.
Another design feature that contributes to carbon efficiency is the cantilevered roof. This unique overhanging roof design profile has more purposes than just looking attractive. Its primary function is to act as a sun shade to screen the direct sunlight on the glazing. The high pitch of the sun in the summer is screened more than the low sun of the winter. This helps balance the demand for sunlight, thus maximising the suns energy when most needed in the cooler winter months and reducing its penetration during the hotter months.
There are many features of our garden rooms that contribute to a greener approach to building. If you are like us and hold the environment dear and are thinking of getting that new extension, be it for work or play, then get in touch with in.it.studios.


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Archive for 2014-07 July Newsletter
Healthy Living Houston: Visit Our Parks Along the Bay
Dr. Anne Hecht, a Texas Master Naturalist, recommends discovering our bays, bayous and estuaries as our healthy outdoor activity for this time of year. Sunset on Galveston Island, photo by Brent Blackett
This month we got in touch with Dr. Anne Hecht, a Texas Master Naturalist, to get her recommendations about healthy outdoor activities for this time of year.
Anne had three great suggestions, all of them related to discovering our coastal bays and bayous.
First on Anne's list is Galveston Island State Park (GISP). What's particularly special about this park for nature lovers is it spans a full cross-section of west Galveston Island — from Galveston Bay on the north to the Gulf of Mexico on the south. Admission is $5 dollars per adult, 12 and under free.
If you want to learn about the bays and marshes, meet at the Nature Center at 10 a.m. on Sunday mornings for a free bay walk tour (included with your $5 admission). If you want to learn about the beach, that tour is conducted on Saturdays, also starting at 10 a.m. at the Nature Center.
Seining demonstration on the bay at Galveston Island State Park. Photo by Nathan Veatch.
New to GISP is a "Car/Hike or Bike Tour" developed by Frank Bowser, who is an active member of The Friends of Galveston Island State Park. Frank made a guide map showing how you can navigate the two miles of paved roadway and the five miles of manicured grassland hiking trails. So get ready for a scenic walk — or bring your bike — to discover all the interesting wildlife in the prairie grasses, brackish marshes and seaside dunes. The map and the companion computer disc are available at the Ranger's Desk at the entrance to the park.
If you want a guided kayak tour of the bay side, once again Frank Bowser is your man. Give him a call at 409-737-5567 and he'll get you set up on a kayak tour. Launch times are timed to hit high tide, so that determines when you go out.
Artist Boat
Anne's second suggestion is to get in touch with another group of outdoor enthusiasts who go by the name Artist Boat.
Artist Boat has a really cool concept: they take kayakers out into the waters of Galveston Bay for an art lesson (painting for example) under the supervision of certified kayaking instructors — who also happen have a professional background in science or art.
If you have no experience kayaking, don't worry. Most first time Artist Boat participants don't have any experience either.
Artist Boat offers an exciting way to discover Galveston Bay: you can go out in one of their kayaks for an art lesson (painting for example) under the supervision of certified kayaking instructors. Photo courtesy Artist Boat.
All the instruction and gear is included in the price of your tour, which ranges from $35 for a short two hour experience (not enough time for an art lesson) to $60 dollars per person for 4 hours, which includes an open air art lesson.
Not only is this great fun, it's for a great cause too. Not that long ago one of the very last significant tracts of un-built land on Galveston's West End was slated to be turned into a housing development.
But when those plans fell through due to the recession, Artist Boat was able join forces with other organizations to acquire a 160 acre parcel of coastal property on west Galveston Island to establish a new Coastal Heritage Preserve.
So when you support Artist Boat, you are also supporting a worthy cause that is protecting open space along Galveston Bay. You can friend Artist Boat on Facebook and see more photos of activities they have going on.
Armand Bayou Nature Center
Anne's third suggestion is the Armand Bayou Nature Center, which is along the west side of Galveston Bay. At 2,500 acres, Armand Bayou is the largest urban wilderness preserve in the entire United States.
We can enjoy it today thanks to a visionary campaign started in the late 1960s by Mr Armand Yramategui, a Basque electrical engineer who ran the Burke Baker Planetarium at the Houston Museum of Natural Science, right across the street from our Museum District office. In 1970 Armand (he went by his first name) was tragically killed in a what we would today call a 'carjacking' incident. The preserve opened in 1974, and Middle Bayou was re-named Armand Bayou in his memory.
Armand Bayou is the largest urban wilderness preserve in the entire United States with over 2,500 acres. Pelicans gather on a tree stump along the bayou. Image courtesy Wikimedia
Armand Bayou has been celebrating its 40th anniversary this year. They received a grant from the Texas Parks and Wildlife Department to rejuvenate some of their hiking trails; this construction activity is nearly complete by now, but you might want to touch base with the office to get the latest details.
The three most popular trails– the Martyn Trail through the forest and along the bayou, the Prairie Interpretive Trail and the Discovery Loop Trail — will remain open during the construction work.
One great way to get an overview of the park is to ride the pontoon boat called the Bayou Ranger. These boat tours start early on Saturday mornings. Enjoy a light breakfast on board as you silently drift down the bayou and catch early morning activities of the park's wildlife. The boat departs from Bay Area Park boat launch and costs $25 for adults. Become a member and save five dollars on your ticket. Make reservations at 281-474-2551 x10.
Another tip is visiting the park on the second Saturday of each month (next one coming up is July 12th, 2014) for two special programs: Photography and Canoeing
You can take a guided photography hike each second Saturday; the start times alternate between early morning and late afternoon. Reservations required, call 281-474-2551 x10 for more information.
You can also take a guided canoe trip (8 a.m. to 11 a.m.) on the second Saturday of each month. Reservations are required, so give a call on 281-474-2551 x10.
Useful Links for Planning Your Healthy Living Houston Outdoor Adventures
The Friends of Galveston Island State Park
Coastal Heritage Preserve
Categories : 2014-07 July Newsletter, Galveston, Healthy Living Houston, Newsletter, Outdoor Activities
Tags : Armand Bayou, Armand Yramategui, Artist Boat, Frank Bowser, Friends of Galveston Island State Park, Galveston Island State Park, GISP, Houston Museum of Natural Science
Take a Look at Houston Events for July 2014
Summer is definitely here, but that doesn't mean you can't get out and enjoy what Houston has to offer. We present our top tips for events and activities in July.
Houston has lots of events and activities to offer in July. Let's go!
Houston Events for Friday July 4, 2014
Revolutionary Celebration at Bayou Bend
Try something different this Fourth of July: Join the Texas Army Fife & Drum Corp, Betsy Ross and other period re-in-actors at Bayou Bend for a historic afternoon celebration. Free. 1 p.m. – 5 p.m. Learn more at Bayou Bend event page.
Houston Events for Saturday July 5, 2014
Paddle down Buffalo Bayou
Paddle down Buffalo Bayou in a kayak and see the downtown Houston skyline in a whole new way. Make your reservation by Thursday, July 3. $60. Kayak Trip event page.
Houston Events for Sunday July 6, 2014
Bike around Houston Bayous, 8 a.m.
Meet at Market Square Park for a 25- to 40-mile bike ride exploring the bayous of Houston. Mountain bikes or bikes with fat tires are necessary. Free. Market Square event page.
Houston Events for Friday July 11, 2014
The Big Show at the Lawndale Art Center
Lawndale Art Center's annual open-call, juried exhibition since 1984. This year juror Erin Elder, Visual Arts Director, Center for Contemporary Arts, Santa Fe, selected 115 works from nearly 1000 pieces submitted. Free. Learn more at lawndaleartcenter.org
Houston Events for Saturday, July 12, 2014
Galveston Art Walk, 6 – 9 p.m.
Every six weeks you can join art lovers from the Island and beyond on a convivial crawl through Galveston's art galleries, antique shops and more. Wine and hors d'oeuvres served by many establishments. Free. Galveston ArtWalk event page.
Houston Events for Wednesday, July 16, 2014
37th Houston World Series of Dog Shows
This five-day extravaganza at NRG Park (the big low-rise convention center north of the old Astrodome) is a great way to learn about different dog breeds. But the secret is it's really about the people watching. Free on Wednesday only, otherwise $15 (cash only). houstondogshows.com/visitors/tickets/
Houston Events for Friday, July 18, 2014
Mixers & Elixirs at the Houston Museum of Natural Science
The Houston Museum of Natural Science cuts loose after dark for this Comic-Con-themed monthly get-together. Enjoy a live band and food from some of the best of Houston's food trucks (bring cash for food trucks) Doors open at 7 p.m. until 10 p.m. $20 or $12 for HMNS members. Press Release
Beyoncé and JAY Z: On the Run
Houston's own Beyoncé and husband Jay-Z are on the run, from what were not sure; possibly the Kardashians after they skipped Kimye's Big Italian Wedding. $96.80 – $800+ Event page
Houston Events for Thursday, July 24, 2014
BCO Pub Stroll and Scavenger Hunt
Here's a great way to get to know the people at the Bayou city outdoors club: join them for a pub stroll and scavenger hunt starting in East Downtown Houston at Lucky's Downtown Pub (on 801 St. Emanuel). This group has dozens and dozens of outdoor activities throughout the year across Houston. Event page
Houston Museum District: The Museum Experience 2014
Four times a year the Houston Museum District Association organizes special programming with its member museums in different zones. This time the focus is on zone three: The Jung Center of Houston, Contemporary Arts Museum Houston and The Museum of Fine Arts Houston. (These are all an easy walking distance from Dr. Brewton's office on Hermann Park Drive.) Visit the event page.
Battle on the Bayou Beard and Mustache Competition
It's time to get your fuzz on for a good cause. Super Happy Fun Land hosts this charity event benefiting Shriner s Hospital for children, brought to you by the Houston facial hair club. Visit Facebook event page.
Categories : 2014-07 July Newsletter, Art Gallery Events, Houston Biking Trails, Houston Museum District, Outdoor Activities
New Food Safety Tips for Cooking Raw Chicken
New food safety recommendations urge cooks to NOT wash chicken in the sink before cooking it. It's safer that way. Learn why in our update below. Image courtesy Steven Depolo, CC License.
It's important to teach young cooks just learning about food safety how important it is to use one set of plates and utensils for preparing and transporting uncooked protein (fish, meat, poultry) to the oven, stove or barbecue grill — then using fresh, clean plates and utensils to serve the cooked food. This avoids transferring bacteria to the cooked food. Now researchers have a new tip specifically for cooking chicken: It's better not to wash your chicken before cooking it!
New Food Safety Recommendation: Don't Wash Raw Chicken in the Sink Before Cooking It
By now, nearly everyone knows that when cooking raw protein like fish, chicken, hamburger or steak, the best practice is to not mix utensils and plates that touch uncooked food with those used to serve the final cooked meal.
What's new is a recommendation from the United Kingdom's Food Standards Agency (FSA) urging people to avoid washing chicken before cooking it. That's right. Don't wash your chicken. It's safer that way.
This seems completely unhygienic and counter-intuitive to most people!
Why Should We Skip Washing Chicken Before Cooking? That Sounds Crazy!
But there is sound scientific logic at work. Here is the back story: Campylobacter is the most common cause of food poisoning in the UK, outranking the more commonly known salmonella, E. coli and Listeria bacteria (all of which cause food poisoning).
The FSA estimates that four in five of the food poisoning cases they have identified came from contaminated poultry. Why is illness from eating chicken so common? Well UK food safety scientists have discovered a key contributing factor. It turns out the step of washing the chicken in the sink before cooking it — which seems so hygienic — actually causes the Campylobacter bacteria to spread around and make you sick.
How does this occur? Scientists discovered that washing a typical chicken in the sink causes a large number of very small liquid droplets — chock full of active Campylobacter bacteria — to splash all over the kitchen sink, adjacent countertop work surfaces and other cooking equipment near the sink. These tiny droplets also end up on your hands and your clothing. So that's how you can unwittingly give you or one of your guests food poisoning.
The irony? If you cook chicken properly to a safe internal temperature of 165 F degrees, the campylobacter bacteria on the chicken that you were trying to remove by washing will be killed off by the heat. Thus, there's no need to wash it beforehand.
Related Food Safety Tips for Buying, Transporting and Storing Chicken
Next time you purchase a whole chicken at the grocery store, take a few precautions.
Take advantage of plastic bags (many stores provide them nearby or you can ask the butcher) and double bag your chicken before you put it into your cart. It's also a good idea to wash your hands at the store.
Once you are home, take more precautions.
If you plan to put the chicken in the freezer, it's a good idea to put it inside a freezer bag (even if it's already in a wrapper) to avoid contamination.
Take care when removing chicken from the freezer. When it's time to defrost your chicken, the refrigerator defrosting method is considered the safest. (It may take a day or two to defrost this way)
Always defrost your chicken by placing in a bowl underneath that's large enough to capture any liquids that might otherwise get transferred to other foods in your fridge. (This is critical for those fresh foods you might eat uncooked, like lettuce or fruit.)
When you're ready to cook a chicken, be sure to clear away any other dishes from the sink. Place the chicken in the center of the sink, and slice open the plastic wrapping with a paring knife or kitchen shears. Drain the liquids. Avoid splashing. Remove the the plastic wrap carefully to avoid drips when transferring it to the trash.
Following these simple steps can help you and your guests avoid a painful bout of food poisoning.
Categories : 2014-07 July Newsletter, Healthy Living Houston, Low Fat Recipes
Healthy Recipe for Zucchini Hamburgers
We received very positive comments on our article "3 Healthy, Low-Fat Recipes to Substitute for Pizza and Pasta" we presented in our last edition of It's a Matter of Health, so we'll continue with the substitutions theme again as we present our "Healthy Zucchini Hamburger Recipe".
Try a Healthy Recipe at the Fourth of July Barbecue
As we look forward to Fourth of July Barbecues, it's hard to fight those cravings for a really juicy hamburgers, pork-chops or grilled chicken.
But this recipe for Zucchini Hamburgers tastes great and it's not all that hard to make. It is a recipe that calls for frying, but if you're careful, you can cook it with a minimum amount of canola oil.
Our recipe is set up to make four burgers, you can double up the recipe by making multiple batches if you want to serve a bigger group.
Let's Start the Quick Prep
First, you'll want to hard boil three eggs in a pan, 8 minutes is plenty. When the eggs cooled down, peel the shells and remove the egg whites, then chop up the whites coarsely and set them aside. We won't be using the yolks in this recipe.
While the eggs are boiling, you'll want to shred 2 cups of zucchini using a mandolin or one of the other clever tools we mentioned in our last recipe. Drop the shredded Zucchini in a colander and squeeze it by hand or with a spatula to remove the liquid. This will make it easier to mix and cook in the following steps.
Find a small bowl and add all the zucchini, along with a finely chopped up medium onion, half a cup of dried breadcrumbs, two lightly beat whole eggs, a dash of salt and a dash of cayenne pepper. After that's mixed together, you'll gently stir in the cooked egg whites that you made in the first step.
Now it's time to cook up our Zucchini Burgers
Take a skillet and put it on the stove over medium low heat. Add a tablespoon of canola oil. When the oil starts to shimmer, it's hot enough.
The next steps are little bit tricky — it's like making drop cookies right on the stove — so you'll need both a spoon and a spatula at the ready. Using the spoon, drop your zucchini mix into the skillet, about two thirds of a cup is perfect. Flatten it into a burger shape with your spatula, and fry it until it's golden brown on both sides (about 5 minutes in total should be good).
If you're an experienced cook with a large skillet, you can probably fry all four at one time. But, if it's the first time you're trying this recipe, you might want to cook one at a time to get a feel for how long it needs to cook on your stove. Also, you may need to add a little more canola oil as you cook the second, third and fourth burger.
Fully Dressed Hamburgers?
The next step is really up to you, it depends on how you like to eat your hamburgers. Before slathering on a lot of mayonnaise or other high-fat content spreads, you owe it to yourself to give it a try without a lot of condiments— maybe just some lettuce, a slice of tomato and a slice of onion on a nice whole-wheat or gluten-free hamburger bun would be perfect.
We think you'll find this an excellent low-fat alternative to regular hamburgers and hope you'll try it at your next Fourth of July Backyard Barbecue.
Here are the ingredients you'll need the recipe:
3 to 5 Fresh Zucchinis (Enough to Make 2 Cups of Shredded Zucchini)
Five Eggs (Three to Hard Boil, Two to Cook with.)
1/2 Cup Dried Breadcrumbs
Dash Cayenne Pepper
Optional Condiments
Four Whole Wheat Hamburger Buns
Four Lettuce Leaves.
Four Slices Tomato
Four Slices Onion
Categories : 2014-07 July Newsletter, Healthy Living Houston, Low Fat Recipes, Newsletter
Tags : Zucchini

redpajama_set_name: RedPajamaCommonCrawl


Consumers might be ready to breathe a collective sigh of relief with the last year's flu season—one of the worst flu seasons in nearly a decade—behind us.
Except that it's not really behind us.
What remains after that dangerous season is growing consumer vigilance and obsession with immune health—a big buzz phrase in today's supplement market. Industry insiders are predicting a boom in sales and innovation.
Global market sales of immune health supplements already are climbing. The sector was valued at $14 billion in 2017 and is expected to reach $25 billion by 2025, according to a forecast by Persistence Market Research.
Why? More consumers are thinking beyond Purell, vitamin C and Airborne, thanks to market messaging and industry-supported clinical studies about how we can boost our immune systems all year long.
The prevailing message is that immune systems can be wrecked by diet, stress, aging and antibiotics, leaving people vulnerable to all sorts of ailments any time of the year. A healthy diet, exercise and meditation are well and good, but supplements may be even better.
The good news is that nutrition for immunity is spread across multiple categories, leaving plenty of room for innovation.
With 80 percent of the immune system located in the gut, the good "bugs" in probiotics are catching on as immune-boosters.
"Consumers are very aware of probiotics. The awareness has grown by 12 percent over the last five or so years," says Megan DeStefano, global probiotics marketing leader for DuPont.
DuPont's portfolio of probiotics for immune health includes its Howaru Protect line for pregnant mothers and infants, kids, athletes, adults, and seniors—five different strains for five targeted populations. DeStefano expects major future growth of probiotics, more products for targeted populations, and many more scientific studies proving efficacy.
In fact, research on the human microbiome already seems to be exploding. DuPont and other major bands, for example, have sponsored multiple clinical trials looking at how various probiotic strains—including DuPont's Bifidobacterium lac tis (Bl-04)—affect people who are exposed to the common cold.
What else is coming? Chocolate pairs well with probiotics. "We see consumers more and more want food-like formats," DeStefano says. Also look for probiotic pairings with other wellness ingredients.
Healthmune+ Junior is one of these blends—a sachet of blended prebiotic and probiotic with cocoa powder. Wellmune, promoted as a prebiotic super fiber, is a key ingredient.
Wellmune, a proprietary baker's yeast beta glucan, has experienced significant growth in the last few years, says Donald Cox, director of research and development.
Wellmune, which was acquired by Kerry in 2015, has benefited not only from working under a bigger umbrella but also from clinical studies such as one earlier this year suggesting that Wellmune may protect intestinal barrier function, even in adults faced with stress. The body's intestinal barrier function allows for the absorption of good things like nutrients and water while defending against toxins and pathogens.
"We are getting people familiar with the fact that what you eat can affect your immune system," Cox says.
Fish oil also could play a big role in the growing immune-health market. Omega-3 fatty acids are expected to be the fastest-growing immune-health supplement ingredient during the next seven years, according to a Persistence Market Research global market study released in April.
While most of the research on DHA and immune health has focused on fetal- and infant-brain development, a new pilot study of adults suggests that concentrated DHA fish oil activates B-cell (white blood cell) responses. That study of obese adults was published in The Journal of Nutritional Biochemistry and sponsored by AlaskOmega, a brand of fish oils.
Glutathione, considered the mother of all antioxidants, is not new to the market, but the sweeping claims of what it can do include everything from immune health to skin lightening.
"There are probably over 800,000 posts on Instagram alone with #glutathione," says Elyse N. Lovett, marketing manager for Kyowa Hakko, which manufactures a branded glutathione ingredient called Setria.
Industry analysts predict the global glutathione market will rise from $182 million in 2016 to $211 million by 2021, according to a market report.
Glutathione protects cells against oxidative damage and stimulates the body's natural killer T cells—the immune system's front-line assassins. The body produces its own glutathione, but levels can be depleted by poor diet, pollution, stress and aging, especially after age 45. It's found in foods, such as garlic, onions and cruciferous vegetables. But the typical diet doesn't include those foods every day, Lovett says.
Watch for more glutathione in products for immune support, especially marketed to older adults, and in supplements for skin health and sports performance.
Immunity supplements are going to get a lot more personal, predict DeStefano of DuPont and Cox of Wellmune.
Immune-boosting supplements are increasingly being formulated for and marketed to specific consumers: aging baby boomers, expectant mothers, back-to-school kids, health-conscious millennials and stressed out career-types.
"The demographics are all over the map," Lovett says.
Expect more sachets (just add water), gummies, shots—beverages, not needles—and even ice pops and other treats you can feel good about eating on the go, he says.
Tiny tonic shots of refrigerated juice, herbs and spices were hot at Natural Products Expo West 2018 and are landing shelf space in natural food stores. Some of the many shots out there include Kor's Wellness Ginger, Ginger Shot Inc.'s Tulua Wellness and Turmeric Wellness, Source Naturals' Immune Support, So Good So You Probiotic Wellness and California Juice Co.'s Immunity with apple cider vinegar.
As for immunity supplements disguised as treats, last summer New Jersey mom Marisa Teiner launched Feel Better Pops—probiotic-laced fruit and herb popsicles that promise "feel better tummy & immunity." Ingredients include ginger and GanedenBC30, a probiotic that can withstand freezing.
"We know ginger not only helps with an upset stomach but it also helps the immune system. We know probiotics restore gut health, but it also is good for your overall immune system," says Teiner, who has a nutrition and culinary background.
The pops started with a quest to help her son, an ice-pop aficionado, recover from a stomach virus. The popsicle Teiner created worked so well that she took it to the Rutgers Food Innovation Center and ultimately created Feel Better Pops. She introduced three flavors to her Tri-State Area at farmers markets, local stores, senior centers and back-to-school events.
Now she's finalizing a deal with Shop Rite and looking for a distributor. A throat-soothing pop and an adult-wellness pop are coming soon.
Teiner and other manufacturers know their immune health claims must be carefully crafted so they don't violate Food and Drug Administration and Federal Trade Commission regulations.
By sticking to vague phrases like "supports immune health" and "seasonal support," brands can avoid making direct claims and still get their messages across.
While makers may be choosing their words carefully, their online reviewers are not—creating a tricky situation with the FDA as the industry grows and attracts more attention.
Justin Prochnow, a leading supplement-law attorney, advises companies to avoid "liking," re-tweeting or otherwise sharing certain customer reviews and testimonials on their Facebook pages, websites and other social media platforms.
In 2014, the maker of Zarbee's children's cough syrup fell into hot water with the FDA after liking customers' comments on its Facebook page. The customers described how Zarbee's products helped them with bronchitis, pneumonia colds, congestion, allergies and insomnia.
The FDA considered the company's "like" as "evidence of intent" to endorse those claims. Expect to hear more about building, boosting and supporting immune health but not curing or treating specific ailments.
With the possibilities for new science, new products and new education about immunity, insiders see plenty of room to grow inside those claim limits. Consumer interest inspires industry innovation. Interest following this recent flu season is high.
If consumers use immunity products and experience fewer colds and infections, the worst flu in years could be the best news ever for the immunity category.


text:
Darryl O'Young claimed his second Macau GT Cup Win at 68th Macau Grand Prix
Craft-Bamboo Racing team Partners Motul to secure the win at the Macau GT Cup
O'Young muscles a battered and rebuilt #95 Mercedes-AMG GT3 to victory
PFAFF PORSCHE MOTORSPORT TEAM WINS IMSA GTD CHAMIPONSHIP
In a championship battle that came down to the final race at Petit Le Mans this past weekend, Pfaff Motorsports captured its first-ever IMSA WeatherTech GTD team and drivers' titles with a second-place finish, capping a tremendous final half of the season. The Canadian team took three victories and two second place finishes in the final five rounds with the no. 9 Motul Porsche 911 GT3 R, affectionately referred to as the "Plaid Porsche."
PFAFF MOTORSPORTS COMPLETES SUCCESSFUL IMSA ROAR TEST WITH NEW DRIVER LINEUP
CANADIAN-BASED PORSCHE TEAM COMPLETES THREE-DAY PRESEASON
SHAKEDOWN OF 911 GT3 R AT DAYTONA INTERNATIONAL SPEEDWAY
Daytona Beach, FL, January 6, 2020 // Following the announcement of a new driver lineup and title sponsorship with Motul, Toronto-based Pfaff Motorsports participated in the annual IMSA WeatherTech ROAR pre-season test at Daytona International Speedway ahead of the season-opening 24 Hours of Daytona, January 25 to 26.
The team's key goals for the weekend included a complete...


Grace Hsieh is an artist, visual designer and social event coordinator. She received a BA in Painting and Calligraphy Arts with a minor in Graphic Communication Arts from the National Taiwan University of Arts. While at NTUA, she worked with the Department of Graphic Communication Arts on a project for the Taiwanese government and later spent time teaching painting and design to aboriginal kids. After graduating, she worked on public sector projects with CSR-Taiwan Summit Brand Marketing and served as a supervisor for projects like the Chiayi City International Band Festival. Grace came to DSI to build on these work experiences and learn strategies to empower communities and address inequality.


Call for submissions drop of dates: June 7-June 11 at Definitely Superior Art Gallery. All Definitely Superior Art Gallery Members, and Die Active Youth Art Collective artists are invited to submit up to two artworks for their participation in this our glittery 28th Anniversary Exhibition, and we want your best as this is going to be a huge and swishy spectacular event, as well an open invitation to the hundreds of artists/musicians/performers and volunteers for a grand and gleeful appreciation for all the work you do! Gala Opening Reception featuring a ferocity of Art-Special Music Performance by Traces-[Pop Montreal]-Projection Mapping + Cake…! and catered fresh summer refreshments. Gallery 1+2. Saturday June 18@7-10PM.
Exhibition runs until July 23rd.


text:
Research Article| Online September 01 1995
Motor Problems in Children With Developmental Coordination Disorder: Review of the Literature
Colleen Willoughby;
Colleen Willoughby
Colleen Willoughby, MSc, OT(C) (R), is Senior Therapist-Research, Child and Parent Resource Institute, 600 Sanatorium Road, London, Ontario N6H 3W7, Canada. At the time this article was written, she was a graduate student in the Department of Occupational Therapy, The University of Western Ontario, London, Ontario, Canada
Helene J. Polatajko
Helene J. Polatajko, PhD, OT(C), is Professor and Chair, Department of Occupational Therapy, The University of Western Ontario, London, Ontario, Canada
Copyright © 1995 by the American Occupational Therapy Association, Inc.
https://doi.org/10.5014/ajot.49.8.787
Colleen Willoughby, Helene J. Polatajko; Motor Problems in Children With Developmental Coordination Disorder: Review of the Literature. Am J Occup Ther September 1995, Vol. 49(8), 787–794. doi: https://doi.org/10.5014/ajot.49.8.787
Occupational therapists frequently work with children with developmental coordination disorder (DCD) in hopes of enhancing their occupational performance. There is debate among researchers and health care professionals about whether the motor coordination problems experienced by children with DCD have a physiological basis or whether they are the result of a developmental delay. Even among researchers who agree that these difficulties are of physiological origin, there is a lack of consensus as to whether these motor problems are multisensory or unisensory. This article focuses on the physiological explanation, presents a review of the literature on the possible physiological origins of motor coordination problems in children with DCD, and shows that the current literature on the physiological basis of DCD requires more empirical evidence to substantiate either multisensory or unisensory theories of motor dysfunction in children with DCD. The debate over the nature of motor problems in children with DCD has two implications for occupational therapists: that there is no one way to treat these children and that the cause of the difficulty varies from child to child.
child, developmental coordination disorder
Pay-Per-View Access


I've interviewed over 400 high performers and top achievers on my podcast and nearly all of them were 35+.
After talking with all of these men over the past half decade I have found a couple of common themes that they ALL followed in order to become hyper successful (many of them were complete failures in their 20's and then turned things around).
Your metabolism slows as you age making it VITAL that you take care of your health now.
Eat 80% whole foods, more greens, drink plenty of clean water, supplement wisely and go to the gym at least 4 times a week. Once a week sweat like you're running for your life.
Consider this an additional 20% tax from uncle Sam.
Put aside 10% of every paycheck into an opportunity fund and the other 10% into a low risk/high yield index fund or something similar. When the market crashes you'll have cash to buy assets at a massive discount. This is how average people can become wealthy.
Seriously, every day for at least 30 minutes read books, listen to podcasts or audiobooks, take online courses, talk to mentors.
You do not get more done with less sleep because you end up doing more average work.
You will perform at least 70% better than you normally do.
Try to be in bed by 11 p.m. and awake between 6:30 and 8:00 a.m. depending on your needs (if you are active you will need more sleep).
It might seem kinda weird at first, but having a men's coach helped me accelerate my life, relationships and business in ways that I can't even count.
Regardless of your current level of success, you NEED a coach to help you identify your blindspots, spot opportunities, and ensure that you stay accountable during your 30's so that you can enjoy your 40's.
All the things you procrastinated on in your 30s you will pay for your in your 40s and beyond.
10,000 years ago, simply making it to 30 years old would be considered a massive "Success".
Today, you aren't a "Success" unless you have a super model wife, 7 figure bank account, and a body like Adonis.
You aren't doing as badly as you think and you can always do better than you realize.
Learn to relax and take it easy on yourself, love yourself, and take care of yourself. Enjoy the present moment otherwise you'll miss it all.
Unless you want to learn what not to do, stop listening to broke unhappy people.
Even if they are your friends or family.
If they don't have the results you want in life then of course show respect but move on and do what you want to do anyways.
In your 20's, you can smoke cigarettes, pound the booze, snort lines, and huff who knows what and wake up the next day and hit the gym strong in the morning. As you break into your 30's this is no longer the case.
Not only is your body no longer able to take the same abuse that it once could, but you are significantly increasing your risk for a premature death if you engage in these vices on a regular basis.
If you want to have a sip of bourbon with some friends or the occasional smoke, let it be. If it becomes a part of your daily lifestyle then you will certainly limit your potential and have regrets in your 40s.
But if you are a slave to any substance or bad habit it needs to get cut out NOW.
Look, life is too damn short to constantly spend it doing things that you hate.
You are not your relationship.
You are not your past mistakes.
If you hate your job, relationship, or city… Change it. Find the lessons you, learn from them and don't look back.
The WORST thing you can do with your 30's is what so many people do with their 20's.
They go through this amazing decade of their lives without actually enjoying it.
They are constantly trying to get to the next thing and eventually they wake up old and dying only to realize that they never truly lived.
You're just 1 doctors visit away from a diagnoses that could severely limit your lifespan or quality of life. We are all victim to this disease we share called being human and there is no escaping it. So while we are here (and don't actually know for how long) live your life in the best way possible that is alignment with your values and what you want out of this beautiful experience we call life.
Vis ta vie de sorte que la peur de la mort ne puisse jamais atteindre ton coeur.
Respecte les autres et leurs points de vue, et demande qu'ils respectent le tien.
Aime ta vie, perfectionne ta vie, embellis toutes choses dans ta vie.
Cherche à rendre ta vie longue et pleine de sens au service des tiens.
Prépare un noble chant du cygne pour le jour où tu passeras dans l'au-delà.
Aie toujours un mot ou un signe de salut en rencontrant ou en croisant un ami, ou même un étranger dans un lieu isolé.
Fais preuve de respect envers tous et ne t'abaisse devant personne.
Quand tu te lèves le matin, rends grâce pour la nourriture et pour la joie de vivre.
Si tu ne vois aucune raison de rendre grâce, la faute en incombe à toi seul.
N'abuse de personne ni de rien, car l'abus change les sages en fous et prive l'esprit de sa vision.
Quand vient l'heure de mourir, ne sois pas comme ceux dont le coeur est rempli par la peur de la mort, qui lorsque leur heure arrive, pleurent et prient pour avoir un peu plus de temps afin de revivre leur vie de façon différente.
Chante ton chant du cygne et meurs héroïquement, tu rentres chez toi.
Tecumseh (mars 1768-5 octobre 1813) était un chef de la tribu des Shawnees. Son nom Tekoomsē signifie « Étoile filante ».


text:
HomeLaw & CrimeCops hunt for 'sadistic' man
Cops hunt for 'sadistic' man
December 31, 2018 Staff Reporter Law & Crime 0
Booted out is the Snr Asst Comm Charamba
HARARE – Police have launched an extensive manhunt for a Harare man who stands accused of causing the deaths of five children after he sickeningly burnt down the home of the family of a 12-year-old girl that he is said to have raped earlier.
This comes after Steven Manjovha, 35, of Budiriro high density suburb, vanished without trace after recently being granted bail by the High Court.
Manjovha — who is facing five counts of murder, two for attempted murder, and a charge of rape — allegedly went berserk on April 12 this year, after he had been accused of raping the 12 year-old girl.
The State says he raped the victim after cornering her at Pension Farm, in Glen View — where she had gone to visit a relative.
Manjovha is said to have dragged the minor into a nearby bush, where he raped her once without using protection.
After the alleged crime, the sadistic Manjovha is also said to have phoned the father of his victim, threatening to harm him and his entire family.
Pursuant to the threats, it is alleged that at around 11pm on the same day, Manjovha went to the victim's home at Churu Farm, where his estranged wife Patricia Rutsato was staying.
When he got there, Rutsato was sleeping with the mother of his victim, together with her five children.
Manjovha then allegedly forced his way into the home, awakening the two women — who tried to fight him.
He was said to be holding a container filled with petrol which he allegedly later used to douse the home, before fleeing.
The five children were severely burnt before they were rushed to Harare Central Hospital together with their mother and Manjovha's wife — where they succumbed to their wounds.


text:
ROTC established at UCI with 'Don't Ask, Don't Tell' in mind
Campus's agreement with US Army includes full nondiscrimination provision, which is perhaps the first in the nation.
When UC Irvine's dean of undergraduate education, Sharon Salinger, began talking with the U.S. Army during the 2009-10 academic year about establishing an ROTC program on campus, the importance of having such a program for students and constituents was clear. UCI students who wanted to participate in ROTC had been traveling to other programs at California State University, Fullerton; California State University, Long Beach; or the University of Southern California. "But it was also important to the campus that the ROTC experience be open to all our students," Salinger said.
She enlisted help from Kirsten Quanbeck, assistant executive vice chancellor and director of UCI's Office of Equal Opportunity & Diversity, who agreed that if ROTC came to campus in the fall, it would have to comply with the University of California's full nondiscrimination policy. It prohibits discrimination based on race, color, national origin, religion, sex, gender identity, pregnancy, physical or mental disability, medical condition (cancer-related or genetic characteristics), ancestry, marital status, age, sexual orientation, citizenship, or service in the uniformed services. That was problematic considering the military's "Don't Ask, Don't Tell" policy, which in 1993 had ended criminal investigations and prosecutions of gays and lesbians in the military ("Don't Ask") but had not ended the prohibition on their serving in the military ("Don't Tell").
But on Dec. 22, 2010, when President Obama signed the bill repealing "Don't Ask, Don't Tell," Salinger, Quanbeck and Kyhm Penfil, campus counsel, knew that regulations officially dismantling the policy would follow. In light of the pending repeal, Salinger began working closely with student and Army representatives to establish the first UCI ROTC program, to begin in fall 2011. Included in UCI's agreement with the Army is the university-wide nondiscrimination policy – making the campus perhaps the first in the nation to have an ROTC program with such a broad nondiscrimination statement.
On Sept. 20, "Don't Ask, Don't Tell" was officially repealed. That same week, UCI's inaugural class of ROTC cadets began participating in the campus's new program, which Salinger is proud to say is open to all.
UCI alumnus Christian Peralta, battalion commander for the program, said he is glad the hard work and good relationships forged among cadets, campus military veterans, student government leaders, and key administrators and faculty members paid off. Now, with an established program in place, Peralta said he wants to help mentor and develop the kind of strong, ethical leaders needed in today's military.
"UCI graduates intelligent and adaptable students needed to be successful in today's world," he said. "The military needs capable, intelligent and adaptable leaders, so one of the aims of establishing a program was to create a connection between UCI students and officer leadership in the military."
About the University of California, Irvine: Founded in 1965, UCI is a top-ranked university dedicated to research, scholarship and community service. Led by Chancellor Michael Drake since 2005, UCI is among the most dynamic campuses in the University of California system, with nearly 28,000 undergraduate and graduate students, 1,100 faculty and 9,000 staff. Orange County's largest employer, UCI contributes an annual economic impact of $4.2 billion. For more UCI news, visit www.today.uci.edu.
News Radio: UCI maintains on campus an ISDN line for conducting interviews with its faculty and experts. Use of this line is available for a fee to radio news programs/stations that wish to interview UCI faculty and experts. Use of the ISDN line is subject to availability and approval by the university.
Taking the 'waste' out of nuclear waste
Orange County expanding COVID-19 testing; 29 more cases reported
'Wicked' star to perform at Medal event
Emerging technologies for tracking COVID-19 in higher ed


text:
package gov.nih.nci.ncicb.xmiinout.domain.bean;

import gov.nih.nci.ncicb.xmiinout.domain.*;
import gov.nih.nci.ncicb.xmiinout.handler.impl.ArgoJDomXmiTransformer;

import java.util.ArrayList;
import java.util.HashMap;
import java.util.List;
import java.util.Map;
import java.util.Collection;

import org.apache.log4j.Logger;
import org.jdom.Element;
import org.jdom.Namespace;

public class UMLModelBean extends JDomDomainObject implements UMLModel {
  private static Logger logger = Logger.getLogger(JDomDomainObject.class.getName());
  private List<UMLPackage> packages = new ArrayList<UMLPackage>();
  private List<UMLClass> classes = new ArrayList<UMLClass>();
  private List<UMLInterface> interfaces = new ArrayList<UMLInterface>();

  private Map<String, UMLTaggedValue> taggedValuesMap = new HashMap<String, UMLTaggedValue>();

  private List<UMLAssociation> associations = new ArrayList<UMLAssociation>();
  private List<UMLGeneralization> generalizations = new ArrayList<UMLGeneralization>();
  private List<UMLDependency> dependencies = new ArrayList<UMLDependency>();

  private Map<String, UMLDatatype> datatypes = new HashMap<String, UMLDatatype>();

  private String name;

  public UMLModelBean(org.jdom.Element element) {
    super(element);
  }

  /**
   * Get the Name value.
   * @return the Name value.
   */
  public String getName() {
    return name;
  }

  /**
   * Set the Name value.
   * @param newName The new Name value.
   */
  public void setName(String newName) {
    this.name = newName;
  }

  public List<UMLPackage> getPackages() {
    return packages;
  }

  public UMLPackage getPackage(String name) {
    for(UMLPackage pkg : packages)
      if(pkg.getName().equals(name))
        return pkg;

    return null;
  }

  public void addPackage(UMLPackage pkg) {
    packages.add(pkg);
  }

  public boolean equals(Object o) {
    if(o instanceof UMLModelBean) {
      UMLModelBean compareTo = (UMLModelBean)o;
      return (compareTo.getName().equals(this.name));
    } else
      return false;
  }

  public List<UMLClass> getClasses() {
    return classes;
  }

  public void addClass(UMLClass clazz) {
    classes.add(clazz);
  }

  public void addInterface(UMLInterface interfaze) {
    interfaces.add(interfaze);
  }

  public UMLTaggedValue getTaggedValue(String name) {
    return taggedValuesMap.get(name);
  }

  public UMLTaggedValue getTaggedValue(String name, boolean ignoreCase) {
    if(!ignoreCase)
      return getTaggedValue(name);

    for(String tvName : taggedValuesMap.keySet()) {
      if(tvName.equalsIgnoreCase(name)) {
        return taggedValuesMap.get(tvName);
      }
    }

    return null;
  }

  public Collection<UMLTaggedValue> getTaggedValues() {
    return new ArrayList(taggedValuesMap.values());
  }

  public UMLTaggedValue addTaggedValue(UMLTaggedValue taggedValue) {
    if(taggedValue == null)
      return null;
    taggedValuesMap.put(taggedValue.getName(), taggedValue);
    return taggedValue;
  }

  public UMLTaggedValue addTaggedValue(String name, String value) {
    UMLTaggedValueBean taggedValue = new UMLTaggedValueBean(null, name, value);

    // add to jdom element
    UMLTaggedValue tv = writer.getUMLModelWriter().writeTaggedValue(this, taggedValue);

    taggedValuesMap.put(tv.getName(), tv);

    return tv;
  }

  public void removeTaggedValue(String name) {
    //remove from jdom element
    UMLTaggedValue tv = taggedValuesMap.remove(name);

    if(tv != null) {
      writer.getUMLModelWriter().removeTaggedValue(this, tv);
    }

  }

//   public static UMLTagDefinitionBean addTagDefinition(Element elt, String name) {
//     Namespace ns = elt.getNamespace();
//     logger.debug("Namespace: " + ns);

//     Element rootElt = elt.getDocument().getRootElement();
//     logger.debug("Root Element name: " + rootElt.getName());
//     Element xmiElt = rootElt.getChild("XMI");
//     logger.debug("XMI Element name: " + xmiElt.getName());
//     Element xmiContentElt = xmiElt.getChild("XMI.content");
//     logger.debug("XMI Content Element name: " + xmiContentElt.getName());

//     List<Element> xmiContentChildren = (List<Element>)xmiContentElt.getChildren();
//     logger.debug("xmiContentChildren Elements found: " + xmiContentChildren.size());

//     Element modelElt = null;
//     for(Element xmiContentChild : xmiContentChildren) {
//       logger.debug("xmiContentChild: " + xmiContentChild.getName());

//       if (xmiContentChild.getName().equalsIgnoreCase("Model")){
//         modelElt = xmiContentChild;
//       }
//     }

//     logger.debug("Model Element name: " + modelElt.getName());

//     Element ownedElement = modelElt.getChild("Namespace.ownedElement", ns);
//     logger.debug("ownedElement name: " + ownedElement.getName());

//     String xmiId = java.util.UUID.randomUUID().toString().replace('-','_').toUpperCase();
//     UMLTagDefinitionBean tdBean = new UMLTagDefinitionBean(ownedElement, xmiId, name);

//     Element tdElement = new Element("TagDefinition", ns);
//     tdElement.setAttribute("xmi.id", xmiId);
//     tdElement.setAttribute("name", name);
//     ownedElement.addContent(tdElement);

//     ArgoJDomXmiTransformer.addTagDefinition(tdBean);

//     return (UMLTagDefinitionBean)tdBean;
//   }

  public void addAssociation(UMLAssociation assoc) {
    associations.add(assoc);
  }

  public List<UMLAssociation> getAssociations() {
    return associations;
  }

  public void addGeneralization(UMLGeneralization generalization) {
    generalizations.add(generalization);
  }

  public List<UMLGeneralization> getGeneralizations() {
    return generalizations;
  }

  public void _addDependency(UMLDependency dependency)
  {
    dependencies.add(dependency);
  }

  public UMLDependency addDependency(UMLDependency dependency) {
    if(dependency instanceof UMLDependencyBean)
      {
        UMLDependencyBean depBean = (UMLDependencyBean)dependency;
        // if dep does not have an element, then we want to add one
        if(depBean.getJDomElement() == null)
          {
            UMLDependency dep = writer.getUMLModelWriter().writeDependency(this, dependency);

            dependencies.add(dep);
            return dep;
          } else {
          throw new IllegalArgumentException("Cannot add an existing dependency");
        }
      } else {
      throw new IllegalArgumentException("Incorrect dependency parameter, expecting class=" + UMLDependencyBean.class + " but received=" + dependency.getClass().getName());
    }

  }

  public List<UMLDependency> getDependencies() {
    return dependencies;
  }

  public void addDatatype(UMLDatatype type) {
    datatypes.put(((UMLDatatypeBean)type).getModelId(), type);
  }

  public UMLDependency createDependency(UMLDependencyEnd client,
                                        UMLDependencyEnd supplier, String name)
  {
    if(client == null)
      throw new IllegalArgumentException("client may not be null");

    if(supplier == null)
      throw new IllegalArgumentException("supplier may not be null");

    return new UMLDependencyBean(null, name, null, client, supplier);
  }

//   public static UMLTagDefinitionBean getTagDefinition(String name){
//     UMLTagDefinitionBean td = ArgoJDomXmiTransformer.getTagDefinitionByName(name);

//     if (td != null){ return td; }

//     td = ArgoJDomXmiTransformer.getTagDefinitionByXmiId(name);

//     if (td != null){ return td; }

//     return null;
//   }

}

redpajama_set_name: RedPajamaGithub


text:
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Third Sunday in Advent – Sister Ginny Scally, SNDdeN
Dec 8, 2021 | Gospel Reflections
For most of my years (and now, that's a LOT of them!), I honestly thought we "rejoiced" in the pink on this Sunday of Advent because we were half-way through this liturgical season! We were that much closer to the celebration of Christmas! Somehow this Advent, however, doesn't seem to offer too many reasons for rejoicing, no matter how close we're getting to Christmas!
There's no need to detail our national and global woes, despite our education, sophistication, determination and cooperation. That litany is too familiar to most of us. What also seems to be familiar these days is that for every political or social step forward, there seems to be a counter-balance of resistance. This reality is not just playing itself out at home. It's happening in nation after nation. It can really be demoralizing. It can really be exhausting. It can really suck the hope right out of our hearts! So, why "rejoice?"
Zephaniah tells us that the Lord is in our midst. Paul tells us that the Lord is near. John the Baptist tells us that one mightier than himself is coming. What the commentary on these Scriptures also tells us though, is the SECRET for our rejoicing! "Yahweh"s presence in the midst of Jerusalem provides DEFENSIVE rather than OFFENSIVE help! Here the protective presence of God contrasts with the (life)threatening presence of death-dealing forces!"
This language acknowledges that the People of God of Zephaniah's time were under attack. Their faith, their hope, their dreams, indeed, even the fulfillment of God's promises seem to be threatened, as ours seem to be today, BUT the Scriptures tell us at the beginning of this third week of Advent, as they told our ancestors of old, that God, through God's grace and the power of God's Spirit, will enable us to hold on to what we believe about justice, equality, the importance of fairness, the care of those made poor, the sick, our elders and the Earth. God, in our midst, will help us sustain our commitments to the imprisoned, to asylum seekers, to global refugees of war and genocide and poverty, etc. while acknowledging and attending to the needs of our own people.
The news worth rejoicing over TODAY then, is that the God we believe in, will strengthen US against discouragement, hopelessness, indifference, cynicism, writing groups of people off or just plain quitting in our efforts altogether! That IS good news worth rejoicing over! We can lean on God in these, our times, and if we allow it, God will sustain US! God will renew us in love. What we need to do, for our part is, "by prayer and petition, with thanksgiving make known our requests to God. Then the peace of God that surpasses all understanding will guard our hearts and minds in Christ Jesus." So… yes… "Rejoice in the Lord always! Again I say it, rejoice! Indeed the Lord is near."
The crowds asked John the Baptist, "What should we do?" He said to them in reply, "Whoever has two cloaks should share with the person who has none. And whoever has food should do likewise." Even tax collectors came to be baptized and they said to him, "Teacher, what should we do?" He answered them, "Stop collecting more than what is prescribed." Soldiers also asked him, "And what is it that we should do?" He told them, "Do not practice extortion, do not falsely accuse anyone, and be satisfied with your wages."
Now the people were filled with expectation, and all were asking in their hearts whether John might be the Christ. John answered them all, saying, "I am baptizing you with water, but one mightier than I is coming. I am not worthy to loosen the thongs of his sandals. He will baptize you with the Holy Spirit and fire. His winnowing fan is in his hand to clear his threshing floor and to gather the wheat into his barn, but the chaff he will burn with unquenchable fire." Exhorting them in many other ways, he preached good news to the people.
Meet Sister Ginny Scally, SNDdeN
Sister Ginny Scally was educated by the Sisters of Notre Dame in her home parish of St. Gregory's in Dorchester, Massachusetts. She joined them after graduating from high school. Like most SND's, her professional life began as a teacher in an elementary setting. Because of her involvement in the parish religious education programs in those early years, she also was influenced by the spiritual needs and hungers of the laity she met. As a result of those encounters, she chose to pursue graduate studies in Theology with an emphasis on Scripture and Spirituality. She was a Pastoral Administrator in priestless parishes (Read "pastoring person!) in the Diocese of Saginaw, Michigan for over 20 years, completed a hospital chaplain's residency and served as the Coordinator of Catholic Pastoral Services at the North Shore Medical Center in Salem, Massachusetts. Ginny is now serving as one of the three Support Coordinators serving in the Northeast Region of the East West Province.
Ways to Join
The Sisters of Notre Dame de Namur value Communication of all its forms! We bond with one another through verbal and non-verbal communications, for building and strengthening our relationships internationally and cross-culturally, and for collaborating with our Associates, Notre Dame Mission Volunteers, Co-workers, and Partners in Mission. We network for the Mission of the Gospel, the Church and our Religious Congregation in making known God's goodness in our time, and in all the places where we serve. We commit ourselves to stand with those made poor by the unjust structures of the society.
We Celebrate the 20th Anniversary of the Global Mission of the Sisters of Notre Dame de Namur at the United Nations!
Gospel Reflections
Second Sunday in Ordinary Time – Sister Marie Andre Mitchell, SNDdeN
All Gospel Reflections
"Ah! How good is the good God."
St. Julie Billiart expressed frequently her special gift of trust in God: « How good is the good God. » Today, Sisters of Notre Dame de Namur make known God's goodness on five continents.
More About St. Julie Billiart


John will also be joined on stage by fellow musicians from his own band. They will play songs from the whole Dire Straits' catalogue including 'Money for Nothing', 'Brothers in Arms', 'Romeo and Juliet' and 'Sultans of Swing'.
You should hurry up for the tickets because already the show scheduled for Teddington for March 15, 2019 is sold out. Tickets are available from John Illsley official website.


The redesigned Bulk Bag Rack includes a swinging gate designed to streamline the process of loading abrasive bulk bags in the rack. The swinging gate has an integrated chain and rope to allow the operator to open and close the gate from the ground. The footprint of the bulk bag rack will fit in the bulk bag rack collars on all Marco bulk abrasive blasting pots. The redesign is another way that Marco is innovating the abrasive blasting industry.


text:
Gravity retaining walls
Permacrib timber crib
Andacrib concrete crib
L-shaped vertical concrete walls
King post walls
Reinforced soil slopes
Textomur reinforced soil slopes
Reinforced soil structures
Modular block reinforced soil structures
Concrete panel vertical
Facing systems
Soil Panel
Gabion cladding
Institutional/public
About Keller UK
About Keller Group
Phi Group is proud to be a leader in retaining structures and we understand that our success is down to the talent, hard work and commitment of our people.
Our work environment
Applying to work with us
We pride ourselves in having some of the best professionals and skilled people in the industry who are motivated by our culture and the opportunities for career growth.
We offer structured training opportunities plus learning 'on the job'
Despite our collective size, we maintain a culture that is both entrepreneurial and family-oriented
Great reasons to work at Phi Group include:
Leading edge projects
We are fortunate to be a part of some of the most exciting and challenging projects in the geotechnical world today, using the latest equipment and techniques.
As well as the chance to learn 'on the job' on some of the most high profile geotechnical projects, we offer structured training and development opportunities which are designed to help our people to realise their full potential.
True empowerment
Our structure and control framework means that our managers are given significant responsibility from an early stage in their careers. People find this highly motivating and it helps us attract and retain some of the industry's best talent.
Competitive rewards and good recognition
We offer competitive rates of pay and benefits and, for many positions, performance-related incentives. And good performance is recognised in a myriad of different ways, not only through our rewards system.
Health safety and wellbeing
We want every person who works for us, or with us, to go home safely at the end of each day. Our Board and senior management are relentlessly focused on achieving our goal of zero injuries and no ill health.
We recognise the areas where our business impacts on society and where we have responsibilities which extend beyond our financial performance. These relate to safety, the environment, our role as an employer and the communities in which we work. By taking these wider responsibilities seriously, we hope to further build on the strong sense of pride our employees have in Phi Group.
Part of a global business offering opportunities for international career growth
Phi Group is part of The Keller Group that has a presence in more than 40 countries across six continents, so we are able to offer international career development for ambitious and talented people who have a global mind-set and are willing to travel.
A learning organisation
A spirit of collaboration and continuous improvement make us a learning organisation. And, through our development and transfer of technologies between our regions and businesses, we can ensure that our engineers have access to the widest range of solutions and the opportunity to continue their technical learning.
Entrepreneurial and family orientated spirit
While part of a larger group, we have both an entrepreneurial and family-oriented spirit.
While our contribution is often unseen, the work we do across Phi Group touches almost every aspect of modern life. We help create the infrastructure for the buildings at the heart of our communities all around the world.
Fair employment practices
We are committed to creating a working environment in which every employee is employed and promoted solely on the basis of his or her merit and personal contribution and one which is free from unlawful discrimination and harassment. We value diversity and celebrate individual differences, believing that our inclusive culture helps the business continue to grow as a strong, dynamic and innovative organisation.
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"I don't think there is anything wrong with a person who is truly overweight wanting and having the goal of losing weight. In fact, a doctor will tell that person to do that--the vast majority of health problems in this country come from obesity, and if losing 30 pounds will allow someone to live a longer and healthier life, I think that is a great goal and should not be looked down upon. I encourage my husband to exercise because I want him to be around longer and not have the same health problems of high cholesterol as his dad. How do you feel about health issues and weight? I have never noticed you to address this issue when talking about body image."
After 35 years of medical practice combating disease and lifestyle flaws, and of watching my own powers ebb, I've stumbled onto two pieces of encouraging news.
I recently ran across this graph, which gives me hope—and pause. When I was in school, American male life expectancy was 69 years. Now that I'm edging up to that cliff and not just a little concerned, out comes a graph like this, which somewhat gratuitously adds another nine years of life. And what's more, those of my age group who managed to escape the ravages of the young: birth defects, hang gliding accidents, homicide, suicide, and military service, average life expectancy reaches 83!
How can this be? What about all the talk of cancer, heart disease, diabetes, violence, environmental decay, unsafe foods, and obesity ruining society as we know it? How can people be living longer each successive year if the health of the nation is going to pot? Is someone playing Chicken Little?
Did I say obesity? I'm an expert, having personally lost 50 pounds three times. The same 50. My daughter Janna, an eating disorder expert, got to me, so I threw away my scales, gave up dieting, started eating intuitively and (I don't know for sure without scales) have accumulated approximately 50 pounds back again, but not without guilt and concern they might jeopardize that magical average life expectancy of 83.
And then came the other piece of good news. This January a large study on BMI (a measure of weight per height) and mortality showed that "overweight" people (BMI from 25-30) actually have significantly lower mortality than people who are underweight, normal in weight, or obese (BMI>30). Further, the "grade I obesity" group (BMI 30-35) had the same mortality rate as the normal weight group. So, increased risk of dying afflicts thin people and very obese people. And a bonus for me: for people over 65, no BMI, high or low, predicts mortality.
Let me bring these findings home to you. Since you likely measure yourself with scales, presumably you're more familiar with weight in pounds than BMI. For a 5'7" woman, the weight range associated with the longest life would be 160-191 lbs. That's right. Just take a moment to ponder that. And we've been calling this category "overweight." For the same woman, 192-223 lbs. would confer the same life expectancy as a "normal" BMI. Doctors call this grade I obesity. I think we should change the names to extra-healthy and pleasantly plump.
Now that is contrary to logic and to everything we doctors have been preaching for decades. (I recognize that some of you readers or your doctors are going to be skeptical. If so, check out the references at the end.) It looks like society's hysterical drive to thinness isn't just driven by fashion, but by the medical profession itself. Maybe doctors' offices need to throw out the scales, too.
This isn't just any study. It is a "meta-analysis" which considers the 141 best research studies on BMI and mortality involving 2.88 million individuals and over 270,000 deaths, and was published in the highly respected Journal of the American Medical Association (JAMA).
So those are good tidings that ease my conscience and my fears. Of course, none of this means that good food and regular exercise aren't linked to longevity. They are. And we will all die someday. Most of us will get sick. It just means we doctors can do more harm than good when we recommend a target weight and dieting to everyone above a certain BMI.
In a letter to the editor of JAMA following publication of this article, Swapna Abhyankar MD and Clement J. McDonald MD chimed in aptly, suggesting to doctors: "…we also wonder if it is time to simply reject the notion that being overweight or mildly obese is always bad for patients and to stop hounding such patients about their weight." Amen.
And me? I'm going to enjoy this pleasantly plump body of mine, feeding it whatever makes it feel loved, making sure that my daily walk is a pleasure, not a duty, and hoping those extra 14 years of life expectancy will count for something.
35 years in medical practice, former Utah Medical Association President, AMA officer, National Family Doctor of the Year, noted medical quality improvement expert, over 30 published academic articles.


Received 2016 October 25; Accepted 2016 November 8.
1. Kim JH, Chung WS, Kang JH, Kim YH, Kim H, Jeon SC. Recurrent bronchoesophageal fistula after endoscopic treatment: a case report. Korean J Thorac Cardiovasc Surg 2010;43:462–5.
2. Von Segesser LK, Tkebuchava T, Niederhauser U, Kunzli A, Lachat M, Genoni M, et al. Aortobronchial and aortoesophageal fistulae as risk factors in surgery of descending thoracic aortic aneurysms. Eur J Cardiothorac Surg 1997;12:195–201.
3. Czerny M, Zimpfer D, Fleck T, Gottardi R, Cejna M, Schoder M, et al. Successful treatment of an aortoesophageal fistula after emergency endovascular thoracic aortic stent-graft placement. Ann Thorac Surg 2005;80:1117–20.
4. Metz R, Kimmings AN, Verhagen HJ, Rinkes IH, van Hillegersberg R. Aortoesophageal fistula successfully treated by endovascular stent-graft. Ann Thorac Surg 2006;82:1117–9.
5. Eggebrecht H, Mehta RH, Dechene A, Tsagakis K, Kuhl H, Huptas S, et al. Aortoesophageal fistula after thoracic aortic stent-graft placement: a rare but catastrophic complication of a novel emerging technique. JACC Cardiovasc Interv 2009;2:570–6.
6. Shin HK, Choi CW, Lim JW, Her K. Two-stage surgery for an aortoesophageal fistula caused by tuberculous esophagitis. J Korean Med Sci 2015;30:1706–9.
7. Hance KA, Hsu J, Eskew T, Hermreck AS. Secondary aortoesophageal fistula after endoluminal exclusion because of thoracic aortic transection. J Vasc Surg 2003;37:886–8.
8. De Masi M, Amabile P, Bal L, Piquet P. Management of endograft infection coupled with aortoesophageal fistula: extra-anatomic aortic bypass and endograft explantation. J Thorac Cardiovasc Surg 2013;146:e11–3.
9. Cho KH, Park JH, Jeong ID, Lee BM, Kim DI, Lee JW, et al. A case of broncho-esophageal fistula treated by histoacryl(R) injection therapy. Korean J Gastrointest Endosc 2005;31:161–5.


text:
package net.bunselmeyer.middleware.pipes.persistence;

import org.hibernate.Criteria;
import org.hibernate.criterion.Order;

import java.util.List;

public interface Repository<T> {

    T read(Integer id);

    RepositoryResult<T> find();

    RepositoryResult<T> find(T example);

    RepositoryResult<T> find(Criteria criteria);

    T create(T entity);

    void delete(T entity);

    void update(T entity);

    public static interface RepositoryResult<M> {

        RepositoryResult<M> limit(int page, int perPage);

        RepositoryResult<M> orderBy(Order... orders);

        List<M> list();

    }
}


If you have cars for sale in Melbourne – Contact us today!At MCR we specialise in paying you cash for your used vehicles, whether it be a car, Ute or truck. If you have old cars for sale in Melbourne or even in rural Victoria, give us a call now and we can offer you a quote. We will remove your car for you and it won't cost you a thing, on the contrary, we will pay you.


Pictures simply click save button to store the pictures for your computer. They are all set for obtain, if you'd rather and wish to obtain it, simply click save symbol on the web page, and it will be instantly downloaded to your laptop computer.
Pictures Lets hope you might want it. That graphic previously mentioned is actually labelled along with.


text:
Y Okada
KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport.
H Yamazaki, T Nakata, Y Okada, N Hirokawa
We cloned a new member of the murine brain kinesin superfamily, KIF3B, and found that its amino acid sequence is highly homologous but not identical to KIF3A, which we previously cloned and named KIF3 (47% identical). KIF3B is localized in various organ tissues and developing neurons of mice and accumulates with anterogradely moving membranous organelles after ligation of nerve axons. Immunoprecipitation assay of the brain revealed that KIF3B forms a complex with KIF3A and three other high molecular weight (approximately 100 kD)-associated polypeptides, called the kinesin superfamily-associated protein 3 (KAP3). In vitro reconstruction using baculovirus expression systems showed that KIF3A and KIF3B directly bind with each other in the absence of KAP3. The recombinant KIF3A/B complex (approximately 50-nm rod with two globular heads and a single globular tail) demonstrated plus end-directed microtubule sliding activity in vitro. In addition, we showed that KIF3B itself has motor activity in vitro, by making a complex of wild-type KIF3B and a chimeric motor protein (KIF3B head and KIF3A rod tail). Subcellular fractionation of mouse brain homogenates showed a considerable amount of the native KIF3 complex to be associated with membrane fractions other than synaptic vesicles. Immunoprecipitation by anti-KIF3B antibody-conjugated beads and its electron microscopic study also revealed that KIF3 is associated with membranous organelles. Moreover, we found that the composition of KAP3 is different in the brain and testis. Our findings suggest that KIF3B forms a heterodimer with KIF3A and functions as a new microtubule-based anterograde translocator for membranous organelles, and that KAP3 may determine functional diversity of the KIF3 complex in various kinds of cells in vivo.
A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally.
Y Sekine, Y Okada, Y Noda, S Kondo, H Aizawa, R Takemura, N Hirokawa
To understand the mechanisms of transport for organelles in the axon, we isolated and sequenced the cDNA encoding KIF4 from murine brain, and characterized the molecule biochemically and immunocytochemically. Complete amino acid sequence analysis of KIF4 and ultrastructural studies of KIF4 molecules expressed in Sf9 cells revealed that the protein contains 1,231 amino acid residues (M(r) 139,550) and that the molecule (116-nm rod with globular heads and tail) consists of three domains: an NH2-terminal globular motor domain, a central alpha-helical stalk domain and a COOH-terminal tail domain. KIF4 protein has the property of nucleotide-dependent binding to microtubules, microtubule-activated ATPase activity, and microtubule plus-end-directed motility. Northern blot analysis and in situ hybridization demonstrated that KIF4 is strongly expressed in juvenile tissues including differentiated young neurons, while its expression is decreased considerably in adult mice except in spleen. Immunocytochemical studies revealed that KIF4 colocalized with membranous organelles both in growth cones of differentiated neurons and in the cytoplasm of cultured fibroblasts. During mitotic phase of cell cycle, KIF4 appears to colocalize with membranous organelles in the mitotic spindle. Hence we conclude that KIF4 is a novel microtubule-associated anterograde motor protein for membranous organelles, the expression of which is regulated developmentally.
Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins.
N Imamoto, Y Matsuoka, T Kurihara, K Kohno, M Miyagi, F Sakiyama, Y Okada, S Tsunasawa, Y Yoneda
Previously, we found that anti-DDDED antibodies strongly inhibited in vivo nuclear transport of nuclear proteins and that these antibodies recognized a protein of 69 kD (p69) from rat liver nuclear envelopes that showed specific binding activities to the nuclear location sequences (NLSs) of nucleoplasmin and SV-40 large T-antigen. Here we identified this protein as the 70-kD heat shock cognate protein (hsc70) based on its mass, isoelectric point, cellular localization, and partial amino acid sequences. Competition studies indicated that the recombinant hsc70 expressed in Escherichia coli binds to transport competent SV-40 T-antigen NLS more strongly than to the point mutated transport incompetent mutant NLS. To investigate the possible involvement of hsc70 in nuclear transport, we examined the effect of anti-hsc70 rabbit antibodies on the nuclear accumulation of karyophilic proteins. When injected into the cytoplasm of tissue culture cells, anti-hsc70 strongly inhibited the nuclear import of nucleoplasmin, SV-40 T-antigen NLS bearing BSA and histone H1. In contrast, anti-hsc70 IgG did not prevent the diffusion of lysozyme or 17.4-kD FITC-dextran into the nuclei. After injection of these antibodies, cells continued RNA synthesis and were viable. These results indicate that hsc70 interacts with NLS-containing proteins in the cytoplasm before their nuclear import.
Identification of diphtheria toxin receptor and a nonproteinous diphtheria toxin-binding molecule in Vero cell membrane.
E Mekada, Y Okada, T Uchida
Two substances possessing the ability to bind to diphtheria toxin (DT) were found to be present in a membrane fraction from DT-sensitive Vero cells. One of these substances was found on the basis of its ability to bind DT and inhibit its cytotoxic effect. This inhibitory substance competitively inhibited the binding of DT to Vero cells. However this inhibitor could not bind to CRM197, the product of a missense mutation in the DT gene, and did not inhibit the binding of CRM197 to Vero cells. Moreover, similar levels of the inhibitory activity were observed in membrane fractions from DT-insensitive mouse cells, suggesting the inhibitor is not the DT receptor which is specifically present in DT-sensitive cells. The second DT-binding substance was found in the same Vero cell membrane preparation by assaying the binding of 125I-labeled CRM197. Such DT-binding activity could not be observed in membrane preparation from mouse L cells. From competition studies using labeled DT and CRM proteins, we conclude that this binding activity is due to the surface receptor for DT. Treatment of these substances with several enzymes revealed that the inhibitor was sensitive to certain RNases but resistant to proteases, whereas the DT receptor was resistant to RNase but sensitive to proteases. The receptor was solubilized and partially purified by chromatography on CM-Sepharose column. Immunoprecipitation and Western blotting analysis of the partially purified receptor revealed that a 14.5-kD protein is the DT receptor, or at least a component of it.
Entry of diphtheria toxin into cells: possible existence of cellular factor(s) for entry of diphtheria toxin into cells was studied in somatic cell hybrids and hybrid toxins.
Y Kaneda, T Uchida, E Mekada, M Nakanishi, Y Okada
J Cell Biol (1984) 98 (2): 466–472.
DOI: https://doi.org/10.1083/jcb.98.2.466
Ehrlich ascites tumor cells were found to be very insensitive to diphtheria toxin. We formed 37 hybrids from Ehrlich tumor cells and diphtheria toxin-sensitive human fibroblasts. The effects of diphtheria toxin on protein synthesis in those hybrids were examined. The hybrids were divided into three groups on the basis of toxin sensitivity. Group A hybrids were as sensitive to diphtheria toxin as human fibroblasts, Group C were as resistant as Ehrlich tumor cells, and Group B had intermediate sensitivity. Group A hybrids had diphtheria toxin-binding sites but Group B and C had no detectable binding sites. Elongation factor-2 of all the hybrids was susceptible to ADP-ribosylation by fragment A of diphtheria toxin. Cells of Group A and B became more sensitive to CRM 45 (cross-reacting material 45 of diphtheria toxin) after they were exposed to low pH (pH = 4.5). The resistance of Group C to CRM 45 was not affected by the same treatment. Group A and B hybrids and human fibroblasts had similar sensitivities to a hybrid toxin composed of wheat germ agglutinin and fragment A of diphtheria toxin, but Group C and Ehrlich tumor cells were resistant to this hybrid toxin. All the hybrids and Ehrlich tumor cells were more sensitive to a hybrid toxin composed of wheat germ agglutinin and subunit A of ricin than were human fibroblasts. On subcloning of Group B hybrids, one Group C hybrid was obtained, but no Group A hybrid. These facts suggest that Ehrlich ascites tumor cells differ from human fibroblasts in the expression of a factor(s) that is involved in entry of fragment A of diphtheria toxin into the cytoplasm after the toxin binds to its surface receptors.
The role of actin in temperature-dependent gel-sol transformation of extracts of Ehrlich ascites tumor cells.
M Ishiura, Y Okada
Ehrlich ascites tumor cell extracts form a gel when warmed to 25 degrees C at pH 7.0 in sucrose solution, and the gel rapidly becomes a sol when cooled to 0 degrees C. This gel-sol transformation was studied quantitatively by determining the volume or the total protein of pellets of gel obtained by low-speed centrifugation. The gelation depended on nucleotide triphosphates, Mg2+, KCl, and a reducing agent. Gelation was inhibited reversibly by 0.5 microM free Ca2+, and 25--50 ng/ml of either cytochalasin B or D, but it was not affected by 10 mM colchicine. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated that the gel was composed of six major proteins with mol wt greater than 300,000, 270,000, 89,000, 51,000, 48,000, and 42,000 daltons. The last component was identified as cell actin because it had the same molecular weight as muscle actin and bound with muscle myosin and tropomyosin. The role of actin in gelation was studied by use of actin-inhibitors. Gelation was inhibited by a chemically modified subfragment-1 of myosin, which binds with F-actin even in the presence of ATP, and by bovine pancreatic DNase I, which tightly binds with G-actin. Muscle G-actin neutralized the inhibitory effect of DNase I when added at an equimolar ratio to the latter, and it also restored gelation after its inhibition by DNase I. These findings suggest that gelation depends on actin. However, the extracts showed temperature-dependent, cytochalasin-sensitive, and Ca2+-regulated gelation as did the original extracts when the cell actin in the extracts was replaced by muscle actin, suggesting that components other than cell actin might be responsible for these characteristics of the gelation.
Reconstitution of lipid vesicles associated with HVJ (Sendai virus) sikes. Purification and some properties of vesicles containing nontoxic fragment A of diphtheria toxin.
T Uchida, J Kim, M Yamaizumi, Y Miyake, Y Okada
J Cell Biol (1979) 80 (1): 10–20.
DOI: https://doi.org/10.1083/jcb.80.1.10
A mixture of HVJ (Sendai virus) spike proteins, the nontoxic fragment A of diphtheria toxin, lecithin, and cholesterol was solubilized in sucrose solution containing a nonionic neutral detergent. The liposomal vesicles which formed on removal of the detergent by dialysis were purified by gel filtration and centrifugation on a sucrose gradient. The resulting purified vesicles had hemagglutinating activity, hemolytic activity and, after solubilization, the enzymic activity of fragment A. The vesicles had no cell fusion activity. Electron microscopy showed that both the outside and inside of membranes of the vesicles were associated with the spikes. When the vesicles were freeze-fractured, no large aggregates of particles were seen on either face. Such fragment A-containing lipid vesicles (liposomes) with HVJ spikes bound to mamalian cell membrane and released their fragment A into the cytoplasm causing cell death. Neither fragment A-containing liposomes without spikes nor empty liposomes with spikes were toxic.