Chicken pot pie was THE comfort food of my mid-century American childhood.
Growing up in New York the great treat was a visit to The Automat on the corner of East 42nd Street and Third Avenue. It was magic - push coins in the slot and a little glass door opens to a freshly baked golden pastry filled with squares of chicken, carrots and peas in a creamy sauce. Push in more coins for the lemon meringue pie. Or maybe the rice pudding. Yum. Now long gone, the last (the one on 42nd street) of a once 30 strong chain in the city closed in 1991, a treasured part of Vanished New York.
My mother never made a pot pie – she was not into pastry nor baking of any kind – but the freezer chests at any supermarket were always well supplied. Even better, family supper recipes like pot pies were the focus of magazines like Ladies Home Journal and Women's Day and anything which made good use of leftovers was always popular. I became a pie baker.
It helped that chicken pot pie is incredibly flexible. Instead of shortcrust or puff (or even filo) pastry, pop baking powder biscuits on top. Or cornbread batter (reputably Oprah's favourite), Or dumplings. Use cooked turkey instead of chicken. And any and as many vegetables you like (freshly cooked or canned). Try gravy from a roast chicken (or turkey) instead of bechamel sauce or cream. And wine (white, or dry sherry or dry vermouth).
If you browse key American cooking websites like NY Times Cooking, Martha Stewart, Food 52, etc., you will find almost endless variations.
Popular history has it that the chicken pot pie was brought to America by English colonists (like my pioneering paternal ancestors, who arrived in the wilds of what came to be North Carolina in the 17th century) but in the many years I have lived here in the UK, I have never been offered one (fish pie, shepherd's pie, cottage pie, pork pie, frequently).
Nigel Slater discovered a delicious "American Pie" at a grand San Francisco hotel. Delia offers her own version, as does Diana Henry in The Telegraph. Felicity Cloake's "How to make the best" series in The Guardian gives a thoroughly researched and undoubtedly excellent variation. The simplest recipe, just leeks, onions and stock embracing the gently cooked chicken, is found in Jane Grigson's "chicken and leek pie from Wales" in her beautiful classic "English Food".
For a basic chicken pot pie, the recipe I most frequently refer to is James Beard's "Old-Fashioned Rich Chicken Pie" in "American Cookery" a lengthy process which starts with poaching a whole bird - perfect when you have the time and are feeding a half dozen or more..
But generally I just make a simple variation of chicken in gravy with whatever vegetables are to hand and top it with bought pastry. Easy for a week night. Cream, dry vermouth, mushrooms and tarragon lift it to delirious heights.
In a small pan, fry chopped onion and mushrooms in olive oil over low heat until softened. Put aside until needed.
Cut chicken breasts into bite sized pieces and place in a small, shallow pan with the vermouth (or sherry). Cover and poach very gently over low heat until cooked.
Add sautéed onions, mushrooms and chopped tarragon to the chicken and season with salt and pepper. Add creme fraisch (or cream) and mix.
Roll pastry to fit a 26cm x 9cm oval oven dish. Place chicken mixture in the dish and cover with pastry. Use a small knife to prick air holes near the centre and brush with beaten egg.
Bake in a preheated 200C/400F oven until bubbling and golden brown - about 30-40 minutes.


Smart and compact, you can take it with you, whether you go to work, do housework, run, or play with your mobile phone.
Due to different producing batches, product details might different slightly.
Due to the different display and different lighting, the picture may not reflect the actual colour of the item.
Warranty period is stated on each deal's description. The product will be exchanged or replaced if there's defect from manufacturer.


text:
Teamster History - Hi Desert Teamster Retirees
Hi Desert Teamster Retirees
History; Part 1
History Part 2
Save our Pension
Health & Welfare, Pension
Teamsters on Facebook
The Teamster; Part 2
Violence Erupts
The first major instance of violence was on May 19 when police attacked a group of strikers who were attempting to stop scabs unloading a truck in the city's market area. The market area became a central location for strike action and violence. Police attacks occurred again on May 21 and 22 when officers and members of the Citizens Alliance advanced on a group of 20,000 workers and supporters trying to stop the opening of the market area.
By this time, many other workers in Minneapolis had followed the Teamsters on strike in solidarity. About 35,000 building workers had walked out in protest of the police violence and many more struck for union recognition.
On May 25, employers in the city accepted many of the striker's demands and worked through other issues with the help of mediators appointed by the governor.
The strikers returned to work, but in a matter of weeks it became apparent that the employers were not abiding by the terms of the agreement. Many union members were fired. Between May and July workers filed more than 700 cases of discrimination. The companies also refused to recognize their agreement to let the union organize inside workers.
The workers again took up the strike on July 17. Three days later, the most violent episode of the strike took place. A large group of unarmed workers were fired on by more than 100 police officers. They had been were lured to a street corner by deputies in a scab truck. The incident became known as "Bloody Friday."
A public commission set up after the strike later testified that "Police took direct aim at the pickets and fired to kill. Physical safety of the police was at no time endangered. No weapons were in possession of the pickets". Two pickets, John Belor and Henry Ness, were killed and the hail of bullets. More than 65 other workers were injured. Many were shot in the back.
The police violence left the working class of Minneapolis stunned, and offers of support and donations flooded in from other unions. Workers took part in strikes to protest the shootings, including a one-day strike of all of the city's transport workers. The Minneapolis Labour Review reported that a crowd of 100,000 people attended Henry Ness' funeral.
Governor Floyd B. Olson immediately declared martial law in Minneapolis, deploying 4,000 National Guardsmen at his disposal. Picketing was banned and scab driven trucks -- issued military permits began to move again.
The union, seeing this as an attempt to break the strike, demanded that all permits be revoked and in defiance of the martial law, the workers vowed again to return to the picket lines on August 1. On the night of July 31, the union headquarters were surrounded and raided by the National Guard troops, who arrested many of the strike leaders.
But rather than hide, the union rank and file called a mass rally demanding the release of the arrested union leaders. Nearly 40,000 people marched on the stockade. The leaders were released and the captured union headquarters was surrendered.
Strike Ended
The strike finally ended on August 21. Through mediation, the employers and Citizens Alliance accepted the union's major demands. Elections were held in workplaces and many more workers joined the union. Many workers also later won major pay increases through arbitration.
The Citizens Alliance had been broken, and with it the backbone of resistance towards union organization in Minneapolis. Workers in many other industries began to organize themselves, and the city maintained a strong union presence throughout the 1930's
The strike was instrumental in building a strong union tradition in Minneapolis and across the Midwest, with a writer of the Minneapolis Labour Review later noting that, "The winning of this strike marks the greatest victory in the annals of the local trade union movement ... it has changed Minneapolis from being known as a scab's paradise to being a city of hope for those who toil."
The Minneapolis strike of 1934 is widely seen as a pivotal moment for the Teamsters and for the labor movement. Membership in the union grew as barriers against "non-craft" workers came down. The union also grew in stature, proving to be a powerful force in the labor movement. The outcome of the strike also led to the enactment of legislation acknowledging the rights of workers to organize and bargain, including the National Labor Relations Act and the Fair Labor Standards Act.
Teamsters and World War II
In times of war, Teamsters have always answered the call to service at home and abroad. During World War I, Teamsters enlisted in the armed forces and helped the military move from cavalry to motorized units. Teamsters not only were skilled drivers, but also were among the few trained to fix motor vehicles of any kind. These skills were crucial in the first battles of modern warfare.
Teamster men and women worked in many capacities on the home front and it was estimated that four out of five Teamsters purchased liberty bonds.
Allied in War Effort
World War II saw an even greater Teamster effort. The Union was an integral part of the Allies' victory in World War II, contributing on the battlefield and on the home front.
In 1942, President Roosevelt asked Teamsters General President Dan Tobin to travel to Great Britain and report back on how British unions were helping to win the war. On his return, Tobin urged the U.S. labor movement to emulate the British approach suspending all labor discord in the face of the Axis' threat to world freedom.
The Teamsters led organized labor in a pledged to refrain from all work stoppages for the duration of the war. Tobin fully subscribed to this policy, maintaining that, "A man who quits work now without the consent and approval of his union -- which he cannot get -- is and should be and will be classed as an enemy of our nation and of our government."
Buy War Bonds
The National Conference of Teamsters was formed to help meet the economic and military crises facing the U.S. by actively promoting war bonds. Teamsters local unions, joint councils, and regional conferences followed suit, raising more than $2 million in war bonds during the first 18 months of the war. And, the Teamsters offered to give the government an interest-free loan of $8 million from its treasury to help win World War II. President Franklin Roosevelt politely declined the offer, but stated, "(Your offer) should be an example to the whole country."
The Teamsters determination to make sure Allied Forces were victorious did not stop there. The union invested 60 percent of its liquid assets in U.S. Treasury bonds during World War II. And it continued to buy bonds as a show of support.
Teamsters General President Dan Tobin summed it up: "We weren't fooling when we told President Roosevelt that all of our assets were behind the government in the war and we weren't fooling when we said this war must be won regardless of cost in men and money. The Teamsters are supplying men and money to the limit of their resources."
The National Committee also organized all kinds of "drives" to help with the war effort, including drives to collect scrap metal and rubber to be used in military supplies. Nationwide, Teamsters took these activities very seriously.
Members of Local 364 in South Bend, Indiana were a perfect example of the Teamster spirit during the war. They collected 2 million pounds of scrap metal from old stoves, fences and other materials that went to the battle fronts in the form of guns, tanks and ammunition. Their effort won national recognition from the War Services Board.
On the Front Lines
By 1942, 125,000 Teamsters were in military operations for the Allied forces. The Allied victory would not have been possible without the Teamsters who drove troops to the front.
Teamster members did not hesitate to volunteer for service after the attack on Pearl Harbor, often going down in groups with other members from their locals to sign up together. They served in every branch of the armed forces, engaging in everything from building the Burma Road and implementing the Red Ball Express supply line in France, to landing on the beaches of Normandy.
Teamsters won scores of medals for bravery and dedication to duty in all times of crisis, including three members who received the highest honor, The Congressional Medal of Honor. Gen. George Patton, Gen. Omar Bradley and the Allied Supreme Commander Gen. Dwight Eisenhower all commended the Teamsters for their dedication, skill and service in the armed forces.
Teamsters at Home
As in WWI, Teamster women did their part for the war effort too. Women took on many jobs previously held only by men and proved they could hold their own in any work setting. They did every job given them and did them well. Teamster women also served in the women's branches of the military at posts in the United States and overseas. Many employers were sorry to lose the women workers when they gave up their jobs to the returning veterans in 1945.
Following the war, the IBT made sure all Teamster veterans kept their seniority when they returned from the war and went back to work. The Teamsters were one of the only groups to do this for their members. A decade-long national campaign, "Have It Delivered," promoted Teamster freight and delivery services, creating more jobs for members returning after wartime service.
Intense organizing campaigns in booming post-war industries such as the automotive trades, food processing, dairy, and workers servicing vending machines were also used to create more Teamster jobs.
The Teamsters have continued to serve their country in many ways in times of war and national crisis. Our members have served honorably in Korea, Vietnam, the Gulf War, Iraq and Afghanistan. Local and Joint Councils continue to support and aide soldiers and veterans as well.
Currently the Teamsters support a program called Helmets to Hardhats, a training program helping returning vets find jobs in the construction industry.
Master Freight Agreement
January 15, 1964 became a monumental day in Teamsters history when the first National Master Freight Agreement was signed in Chicago. This contract would provide solid, standardized protection and benefits to more than 450, 000 over-the-road and local cartage drivers across the country.
The contract, which was described as an impossible task by critics and friends of labor alike, was a milestone for labor unions everywhere. James R. Hoffa, architect, chief negotiator, and overall firestorm of energy behind the agreement, considered this the crowning achievement of his tenure with the Teamsters.
A Dream Becomes Reality
The NMFA of 1964 brought more workers into the middle class than any other single event in labor history. Hoffa was determined to improve the standard of living for workers, and increase respect for "non-craft" laborers such as over-the-road drivers.
The agreement was the first step in a larger dream to nationalize union contracts. And it revolutionized the way in which goods of all types were moved across the country.
Over-the road and cartage drivers -- empowered through economic gains, stability, and a strong Teamsters Union -- became a force in America's political landscape. Political leaders began to realize the concerns and interests of this group must be recognized, as they could easily mobilize into a formidable voting block as never before imagined.
Skip back to navigation
Footer secondary sidebar
Proof of Our Quality
Everybody is a genius. But if you judge a fish by its ability to climb a tree, it will live its whole life believing that it is stupid. Albert Einstein
Passports – Visas
Home/Auto Insurance
Union Legal Trust
Calif Senior Driver
Harley – Davidson
WCT Prudential
Teamsters JC42
Teamsters Local 63
© 2012-2019 Hi Desert Teamster Retirees | Donated and Powered by a Retired Teamster

redpajama_set_name: RedPajamaCommonCrawl


Broken auto glass is no problem for The Windshield Guru Team. Get your broken windshield fixed before the WV highway patrol comes after you. Review many glass repair firms in lost city, WV Leading Glass Technicians has years of experience with all makes of cars. Our unique technology makes it simple to get your windshield fixed.


The 25-foot wooden ski jump was dedicated on January 14, 1911, making St. Olaf one of the first U.S. colleges to offer ski jumping. By January 1913, an upgraded 72-foot metal scaffold was erected and named in honor of Anders Haugen, the 1910 U.S. national ski jumping champion and world record holder.
Annual tourneys attracted the best amateur skiers from across the northwestern United States and Norway, garnering 1,500 or more spectators. In 1930, the Haugen Ski Jump was modified essentially for one student, Lloyd Ellingson, who became national intercollegiate ski champion. He was also a member of the 1932 Olympic ski team.
Officially the ski jump record of 90 feet was set by Wilbur Najjar, a skier from the University of Minnesota Duluth. Another skier from the University of Minnesota Duluth, Joe Nowak, leaped 94 feet, but landed in the crowd and fell, so his jump did not reach the record book.
One of the greatest campus hoaxes took place in 1946 at the groundbreaking of Thorson Hall. Officials and spectators were horrified to see a body hurtle from the top of the slide and plunk to the ground below. Pandemonium ensued as the crowd rushed to the scene. An ambulance was summoned and arrived quickly with siren screaming. All this help was for a dummy, fashioned and tossed off the slide by prankster students.
By February 1961, college officials had the venerable wooden ski jump permanently removed; it was determined to be potentially unsafe for neighborhood children. Disuse due to mild winters in the previous several years contributed to its demise. In addition, development of the athletic area below the jump had changed the grading of the landing slope's outrun, making it unusable for ski jumping.
Agre must have learned a few things from launching basketballs down the slide—he was awarded the 2003 Nobel Prize in chemistry.
Popping off on Pop Hill Ski Jump.
Original Wooden Ski Jump, a.k.a. Haugen Ski Jump, ca. 1913.


After my mattress crossed the year mark, I knew it was time to get a new mattress. However, it seems that things have changed a lot in the mattress industry since the last time I bought a mattress. So I went online, to find out which mattress would suit my needs (chronic back pain) and my wallet. Interestingly, I found plenty of positive Memory Foam Mattress Reviews from all over the world.
A memory foam mattress is made from polyurethane foam which softens on contact with the body heat and offers to contour to any body shape. The mattress conforms to the shape of your body as soon as you lay down and it will also regain its shape when you get up from the mattress. It is considered to be extremely beneficial for people suffering from body aches and pains. With my chronic back pain, this seemed like the right choice for me. While making the final decision, I had to decide between a King Size Memory Foam Mattress and a Queen Size Memory Foam Mattress. Since my bedroom was on the larger side, I decided to get the former. You can also get a memory foam mattress for a single bed. The online seller delivered the mattress and helped set it up in my room within 2 days.
Although excited about the new mattress, I was a bit wary about using it. I was wondering whether I should have gone for a Natural Memory Foam Mattress India instead. The first night was a bit uncomfortable as the new mattress seemed a bit firmer than I expected. However, I had read online to expect this, especially since I am on the lighter side. The online seller also offered a return policy till 100 days, in case I was not satisfied with the mattress. So I was not too worried. I continued using the mattress for about two weeks when I started noticing a slight difference. The mattress seemed to contour to my body shape much more easily. I also experienced zero to very little back pain after getting up in the morning. Hallelujah! I was finally able to get a good night's sleep after what seemed like ages. This, in turn, helped improve my work efficiency the following day. I also found myself not feeling tired towards the end of the day, like I used to earlier. The memory foam mattress had undoubtedly made a difference in my life, day and night!
If you are looking for a new mattress, I would recommend investing in the Best Memory Foam Mattress India to get the full benefit from it. In case you don't have a budget for a new mattress or just bought one recently, you can also consider getting a Memory Foam Mattress Topper. They will convert your current mattress into a memory foam mattress at a fraction of the cost. In order to extend the life of beloved memory foam mattress, I have been advised to rotate it once a month. This will help prevent the mattress from sagging quickly.


text:
The Wills Group Selects Tanglewood Park in Clinton, MD for Revitalization
Alongside community leaders and partners, Wills Group executives and our Community Engagement Committee members chose Tanglewood Park in Clinton, Maryland, as the next neighborhood revitalization project for the company's pillar Community Engagement program, Reimagining Outdoor Spaces.
For nearly 100 years, the Wills Group has been committed to serving the diverse communities in which we live and operate. We express this as keeping Lives in Motion—moving our customers, neighbors, and employees forward toward their goals and dreams.
At the forefront, we're helping customers at both our Dash In and Splash In ECO
Car Wash stores meet basic needs that allow them to pursue greater goals and ambitions. Behind the scenes, we're engaging with employees and community members to identify opportunities that will help our communities thrive.
Reimagining Outdoor Spaces: Our Process
Reimagining Outdoor Spaces is one of our signature Community Engagement programs in which we work with community members to transform a neglected or underutilized outdoor space.
The selection process and enhancement decisions are almost entirely community driven. The Wills Group and our community partners conduct surveys and focus groups to learn first-hand what the community needs for the outdoor space. Along the way, we form the project's scope and design enhancements with input from community members and seek final approval from local authorities.
Tanglewood Park will be our second outdoor revitalization project. The first, Phoenix Run Park in La Plata, Maryland, was completed in June 2022 after two years of collaborative efforts from local businesses, community members, and Wills Group employee volunteers.
The Wills Group invested more than $130,000 into the Phoenix Run Park transformation, along with contributions from local businesses. The space now features art-inspired basketball courts, walking paths, family seating areas and fresh landscaping. What was once a neglected neighborhood park is now a showpiece of community pride and recreational function.
Our process for Reimagining Outdoor Spaces aims to include as much input from the community as possible. We believe that a collaborative and inclusive approach will result in a better design, greater participation, and collective pride of ownership among park visitors.
About Tanglewood Park
Tanglewood Park is a 10-minute walk from our Clinton, Maryland, Dash-In store. The park, accessible by car or by public transit, is just minutes from Route 5.
Currently, amenities at Tanglewood Park include sports fields, playgrounds, picnic areas, trails, as well as basketball and tennis courts. The park is especially favored by youth and adult sports teams.
The Wills Group, Neighborhood Design Center, and Maryland-National Capital Park and Planning Commission (MNCPPC) have recently conducted two data collection days, in addition to a survey to determine which improvements would benefit park-goers the most. The survey identified our top five amenities for consideration:
Trail mile markers
Benches and seating
Art incorporated into the basketball courts
New grills in the picnic area
Accessibility improvements at the playgrounds
Final approval for these improvements will be determined by the MNCPPC.
Why Outdoor Space Matters
Thoughtfully designed outdoor spaces make communities safer, happier and healthier. They bring people together and encourage an active lifestyle. They increase property values and drive further neighborhood investment. They support mental health and reduce neighborhood crime. Preserved green spaces are also beneficial to the environment, providing groundwater filtration and absorbing less heat from the sun.
The Wills Group is passionate about helping our communities thrive. By investing in park projects like Phoenix Run and Tanglewood, we're helping to build stronger communities that enable its residents to pursue their goals and dreams more readily. This is the driving force behind Lives in Motion and an expression of our core values and purpose.
We're eager to embark on our second outdoor space project at Tanglewood Park and our energetic staff are ready to breathe new life into this cherished public venue. We welcome you to follow along as we blog about our Tanglewood Park journey.


text:
Q: Creating a DLL from libs without any new code I have a dll that uses managed c++ and consumes a native c++ library. This dll is then in turn consumed by a c# interface. As you probably guessed, the managed c++ is just interop code. Nothing in this is exported.
We have a new requirement that both the managed c++ and native c++ be accessed by an external c++ executable (for internal testing purposes). So I was thinking of changing the managed c++ layer to a lib, and have a separate project that creates the dll by consuming the managed and the native libs. There wouldn't be any new code in there. It's just a project that consumes the two libs. Then, we could have another project that also consumes these two libs and adds on top a bit of test code. But I'm having a great deal of difficulty in getting this to work. I cannot get the IDE to generate my dll. Does anyone have any suggestions for me?

A: You cannot create a static link library from managed code, there's no support for it in the VS build system.  The unit of storage for managed code is an assembly.  They are not linked at build time, it happens at runtime.  Beware that your test program needs to be managed in order to test your wrapper.

redpajama_set_name: RedPajamaStackExchange


text:
Автошля́х Т 0434 — автомобільний шлях територіального значення у Дніпропетровській області. Пролягає територією Софіївського та Криворізького районів через Ордо-Василівку — Сергіївку — Кривий Ріг — до перетину з . Загальна довжина — 26,3 км.

Маршрут
Автошлях проходить через такі населені пункти:

Джерела
 Постанова Кабінету Міністрів України від 18 квітня 2012 р. № 301 Київ Про затвердження переліку автомобільних доріг загального користування державного значення
 Про затвердження переліку автомобільних доріг загального користування державного значення: Кабінет Міністрів України; Постанова, Перелік від 16.09.2015 № 712

Т0434
Територіальні автомобільні шляхи України
Транспорт Криворізького району
Транспорт Софіївського району
Транспорт Кривого Рогу

redpajama_set_name: RedPajamaWikipedia


Hip-hop sensation Cardi B has revealed she often feels like "the world owns her" in a cover interview with CR Fashion Book.
Pink nails the national anthem at the Super Bowl all while battling the flu!
Happy Days star Scott Baio has been accused of sexual abuse by actress Nicole Eggert.
Prince Harry's finance Meghan Markle plans to break a centuries-old tradition when she weds the royal in May.
Beloved Frasier star John Mahoney has died at age 77. The actor passed away on Sunday in Chicago.


Spend time transforming tiles, broken crockery, stone, granite and other materials into a mosaic you can treasure for your home or garden. These sessions are held in my studio in Macleod and provide an introduction to the picassiette style of making mosaics.
This is a cost-effective, sustainable and creative way to salvage items that would otherwise be discarded.
Mosaics can be made by all kinds of people and no experience is necessary. Even children as young as five years old can assemble and glue pieces onto wood or a tile to make something colourful and joyful.
Many people find the process of making mosaics very relaxing and meditative and time just slips away.
$50 per session including materials and refreshments.
Max. 5 children per session.
Discounts for groups of two or more.
Please call Amanda to discuss details.
These sessions aim to give you an introduction to the basics of mosaics, to get your brain and hands working to make patterns in a relaxing and supportive environment.
Upcycled crockery, tiles, glass and other items.
People work at different paces, depending on whether they have already contemplated and drawn up what they would like to make. Some may finish their piece in one workshop. Others will need a follow-up session to complete their work. Grouting, if needed, is done at least 24 hours afterwards.
One-on-one mosaic or grouting session - by appointment.
Enthusiasm and a willingness to learn. If you have an old cup, plate or other item you would like to include in your mosaic, please bring this along.
Wear solid shoes, and comfortable clothing. Optional items: apron, rags, water bottle.
To secure your spot in a workshop, payment is due one week before the workshop date.
A 25% cancellation fee will be charged if you cancel within a week and can't come another time.
Please register your interest for other workshops via the contact form or call Amanda 0413 997 467.


text:
Prince Harry urges momentum to end AIDS
Home » HIV AIDS » Prince Harry urges momentum to end AIDS
Complacency threatens to halt progress in the fight against HIV, but through his charity Sentebale, Prince Harry is encouraging a new generation to take up the mantle.
"When my mother held the hand of a man dying of AIDS in an East London hospital, no one would have imagined that, just over a quarter of a century later, treatment would exist that could see HIV-positive people live full, healthy, loving lives," says Prince Harry.
The prince has continued his mother's work by setting up the charity, Sentebale, in 2006, with Prince Seeiso of Lesotho, to support the mental health and wellbeing of children and young people affected by HIV in southern Africa.
A quarter of a century since his mother's pioneering work began, thanks to the work of Sentebale and other organisations tackling HIV-related stigma and discrimination, the virus is no longer the death sentence it once was. However, that does not mean the HIV/AIDS community's work is done – far from it.
In the UK, infection rates are still rising in some groups.
Complacency threatens progress
"We now face a new risk – the risk of complacency," says Prince Harry.
"As people with HIV live longer, AIDS is a topic that has drifted from the headlines. And with that drift of attention, we risk a real drift of funding and of action to beat the virus."
The facts speak for themselves. HIV remains a leading cause of death for adolescents in sub-Saharan Africa and, within the UK, infection rates continue to rise in some groups despite the availability of instant testing and universal access to treatment. The fear of discrimination continues to deter people from testing for HIV and seeking life-saving treatment.
Prince Harry, with youth advocate Tlotlo and Professor Peter Piot, Director of LSHTM, in July 2017. Photo © Chris Jackson/Getty
Empowering a new generation
If we are to maintain positive momentum and push forward to an AIDS-free future, Sentebale's co-founding patron, Prince Harry, believes we need to listen much more closely to young people living with HIV.
"What I believe is that we cannot beat HIV without giving young people in every country the voice they deserve. Without education and without empowerment, HIV will win," he says.
Focussing on physical effects isn't enough; wider social and emotional needs are often overlooked.
This approach is one the prince has championed through Sentebale. Research shows that young people in southern Africa, who are in need of support for HIV, not only face many barriers in their quest to access care, but that their wider social and emotional needs are often overlooked in the process.
"We knew that just focussing on the physical effects of the disease wasn't enough. I've seen first-hand the amazing progress that has been made in treating the physical and mental effects of HIV," he explains. "Seeing young people who have so little, yet who work so hard to support their friends and educate their families about HIV, continues to inspire all of us at Sentebale. They are why I care so much about this fight."
Earlier this year, Sentebale launched 'Let Youth Lead.' This is an advocacy programme to give young people in southern Africa, who are affected by HIV, a platform to voice their challenges and engage policymakers to drive positive change in HIV interventions that better support their generation.
Prince Harry, with youth advocates at Sentebale's Let Youth Lead Roundtable at the London School of Hygiene and Tropical Medicine. Photo credit: Chris Jackson/Getty images
Ending the epidemic for good
As Prince Harry continues the pioneering work his mother began, he wants to galvanise support for young people to break down barriers – such as a lack of youth-friendly health services – that are preventing them from knowing their HIV status and accessing life-saving treatment once and for all. Prince Harry and Sentebale are determined to advance the progress that has been made and to extend vital support and services to the most marginalised youth across southern Africa.
It is time for a new generation of leaders to step forward.
"It is time for a new generation of leaders to step forward," concludes Prince Harry. "In helping young people fight HIV we would not just be ending this epidemic, we would change the direction of history for an entire generation."
This commentary is taken from past engagements Prince Harry has undertaken with Sentebale. This is not new or exclusive content.
Kate Sharma


Product prices and availability are accurate as of 2019-04-05 01:21:20 UTC and are subject to change. Any price and availability information displayed on http://www.amazon.com/ at the time of purchase will apply to the purchase of this product.
With anti-slide middle zipper that pet and family members can share the back seat together.
Built-in velcro openings for your seat belts ,to ensure your pets safe on the way.
You can hand wash or wipe with a damp cloth, but when you wash it by machine, please pay attention to the clasps and the seat anchors.
Just snap the buckle straps around the headrest and backrest, insert the seat anchor in between seat cushions and dress the bottom seat skirt.Then adjust it properly.


$2,809 off MSRP! 8-Speed Automatic, 4WD, Black Cloth.
Red Tintcoat 2019 Chevrolet Silverado 1500 RST 4WD 8-Speed Automatic EcoTec3 5.3L V8 Price includes: GM Employee Pricing $1,500 - GM Down Payment Assistance Program. Exp. 04/30/2019 Price includes dealer added accessories.


text:
Florence turns deadly, unleashing 'catastrophic' floods on Carolinas
Published Saturday, September 15, 2018
Tropical storm Florence caused "catastrophic" flooding in the Carolinas Saturday even as it began to weaken, after resulting in multiple deaths, including a woman and her baby killed when a tree fell on their house.
Four deaths were confirmed by officials as American media reported another, and the storm has wreaked havoc in the Carolinas, lashing the US states with torrential rain and causing rivers to burst their banks.
"Florence slowly weakening just inland over eastern South Carolina but causing catastrophic flooding over North and South Carolina," the National Hurricane Center (NHC) said early Saturday morning, after downgrading it from a hurricane to a tropical storm the previous day.
North Carolina Governor Roy Cooper had said that "several more days of rain" were expected, describing the amount of rainfall from the hurricane as a "1,000-year event."
"Into next week our rivers are going to continue to rise and there will be more significant flooding," Cooper said.
There had been three confirmed deaths from the storm and several others were being investigated to determine if they were storm-related, he said.
The mother and her baby were killed in New Hanover County when a tree fell on their house, the governor said, while confirming another death in Lenoir County.
Local authorities reported a death in Pender County when downed trees prevented emergency units from reaching a woman with a medical condition.
Local media said she had suffered a heart attack.
US media later said a second man in Lenoir County died after heavy winds knocked him down as he tried to check on his dogs.
"This storm is going to continue its violent grind across our state for days," Cooper said.
More than 760,000 customers in North Carolina were without power and 21,000 people were being housed in 157 shelters across the state.
The White House said President Donald Trump was to visit hurricane-hit areas next week "once it is determined his travel will not disrupt any rescue or recovery efforts."
"Great job FEMA, First Responders and Law Enforcement - not easy, very dangerous, tremendous talent. America is proud of you," Trump tweeted on Friday, referring to the Federal Emergency Management Agency, which responds to disasters in the United States.
Mother and baby fall victim to Florence's wrath
Taking cover as Hurricane Florence unleashed fury on the US port city of Wilmington, Adam Sparks heard "a loud pop."
He went to the door to see what had happened, but "it was still pitch black at that time," he said. Power had gone out just before the powerful storm's eye touched down in his state of North Carolina early Friday morning.
It was only an hour later, when fire trucks had taken over the street, that he realized the worst had happened: a mother and her infant had died after a tree struck the back of their house.
"Most of the neighborhood had evacuated," Sparks said. "Only myself, themselves and a few others decided to stay."
Their small home with red brick walls appeared intact from the street - but the back offered a more grim view.
A section of wall had completely collapsed, with a few belongings in view bearing witness to the violence of the crash.
Later in the day Wilmington Police confirmed the mother and child were the first fatalities resulting from Florence's onslaught, adding that the father was taken to the hospital with injuries.
"The tree line they have behind their home is very tall, very old trees," their neighbor said. "It's always a gamble with trees like that."
"Who could be prepared for this? It's the worst-case scenario."
Hurricane Florence makes landfall in North Carolina
Hurricane Florence made landfall near Wrightsville Beach, North Carolina at 7:15 am (1115 GMT) on Friday, the National Hurricane Center said.
"Hurricane Florence made landfall minutes ago," NHC Director Ken Graham said in a Facebook Live video.
Hurricane Florence weakens to Category 1 storm
US forecasters late Thursday downgraded Hurricane Florence to a Category 1 storm, as its outer edge lashed the Carolinas with strong winds and heavy rain.
In its 0300 GMT advisory, the National Hurricane Center said the maximum sustained winds of Florence were 90 miles per hour (150 kilometers per hour), dropping it to the weakest of five categories on the Saffir-Simpson scale.
But the storm still carried "very dangerous winds," the Center added.
On Wednesday, Florence was downgraded twice from its peak as a Category 4 storm which causes "catastrophic damage."
But Brock Long, the administrator of the Federal Emergency Management Agency, has warned residents to take the storm seriously no matter what the category.
Forecasters continue to warn of storm surges, excessive rainfall and catastrophic flash flooding.
Hurricane Florence closes in on US east coast
Hurricane Florence was on course Wednesday to deliver a powerful blow to the east coast of the United States, with forecasters warning of life-threatening rainfall and flooding even as it weakened to a Category 2 storm.
Georgia joined four other coastal states issuing an emergency declaration as forecasts showed Florence dumping historic amounts of torrential rain on the southern state.
As Florence closed in, President Donald Trump and state and local officials urged residents in the path of the storm to evacuate before it was too late.
Appeals to stay safe came from as far away as space as German astronaut Alexander Gerst tweeted pictures of the monster storm taken from the International Space Station along with the warning: "Watch out, America!"
The National Hurricane Center (NHC) said that Florence's maximum sustained winds had eased to 110 miles per hour (175 kph) and it had been downgraded to a Category 2 storm from Category 3 on the five-level Saffir-Simpson wind scale.
It was the second such drop in the space of hours, but the Miami-based NHC stressed it remained "a life-threatening situation" due to the risks of storm surge around coastal areas.
Up to 1.7 million people are under voluntary or mandatory evacuation orders, and coastal residents were frantically boarding up homes and businesses and hitting the road on Wednesday as the storm approached.
"Get out of its way, don't play games with it, it's a big one, maybe as big as they've seen," Trump said. "We'll handle it. We're ready, we're able.
"Protection of life is the absolute highest priority," he added.


text:
The Walking Dead: New Webisodes Announced
2013/09/26 20:03:57 +00:00 | Jonathan James
Robert Kirkman confirmed that a new set of webisodes were in the works during Comic-Con, but now it has been officially announced by AMC. We'll get three new webisodes next week, starring Ashley Bell, Wyatt Russell, and Ellen Greene:
"AMC's award-winning "The Walking Dead" webisode series returns to www.amc.com and AMC's YouTube Channel, www.youtube.com/amc October 1 with three new installments. The newest series – "The Oath" – was executive produced, directed and conceived by Greg Nicotero, executive producer, director and special effects makeup designer on the hit show.
In the three webisodes (7 to 10 minutes each), the lone survivors of a massive walker-attack, Paul (Wyatt Russell, Cowboys and Aliens) and Karina (Ashley Bell, The Last Exorcism), speed across the countryside, frantically searching for an aid-station, as one of them slowly bleeds out from a wound. Finally coming upon a hospital that has not been overrun, at first feels like a blessing, but could soon turn to misfortune, as a seemingly benevolent doctor (Ellen Greene, Little Shop of Horrors) may hide a darker agenda.
Right on the heels of directing season 4's premiere episode, Greg Nicotero focused his sights on the most ambitious "The Walking Dead" webisode series yet. "There's walkers, gore and new threats lurking around every turn -- all the elements that fans 'The Walking Dead' will love!"
Nicotero further commented, "The webisodes give us a unique opportunity to see other parts of the ravaged world of 'The Walking Dead' while weaving in and out of our show. The crossover between 'The Oath' and 'The Walking Dead' ties in one of the most iconic images from the series, and gives us insights and history to yet another timeline. I had worked with Ashley on The Last Exorcism and brought in Wyatt and Ellen, who rounded out the brilliant cast perfectly."
"'The Walking Dead' audience is passionate and enthusiastically interacts with the show and other fans online. Being able to develop new ways for them to experience the world of 'The Walking Dead,' like these webisodes, is a no-brainer," said Mac McKean, senior vice president, digital media, AMC. "They tell a great story with high production values, and they perfectly complement AMC's extremely popular 'The Walking Dead' digital experiences -- games, second screen experiences and interactive content."
The previous two seasons of amc.com's "The Walking Dead" webisodes have won back-to-back WGA, Streamy and Webby awards. The webisodes are produced by Alloy Digital/Generate with Chris Pollack, Jared Hoffman, Greg Nicotero and Michael Petok serving as executive producers.
Hyundai continues its partnership with AMC and "The Walking Dead." From the iconic Kiwi Green Tucson to Hyundai's growing fleet of Zombie Survival Machines, including a fan-designed version to be revealed at New York Comic Con, the brand continues to play an important supporting role in "The Walking Dead" fandom.
"It's great to extend our partnership with AMC again for 'The Walking Dead' webisode series," said David Matathia, director, marketing communications, Hyundai Motor America. "We know fans can't get enough of 'The Walking Dead' world and we're excited to provide unique content before the start of the season."
"The Walking Dead," the most watched drama in basic cable history, returns for another terrifying and exhilarating season on Sunday, October 13th at 9pm ET/PT. In this highly anticipated new season, we see Rick and the group of survivors fostering a thriving community in the safe haven of the prison. Sadly, in this brutal world, happiness is short-lived and walkers and outside threats are no match for danger brewing inside the fences. The group's home and new way of life will be thoroughly tested, and their struggle to survive has never been so perilous. Season 4 also introduces several new characters including Bob Stookey (Lawrence Gilliard Jr., "The Wire").
The premiere episode entitled "30 Days Without An Accident" was written by Scott M. Gimple and directed by Greg Nicotero."
We've included the first photo from the new webisodes below. Look for more content from The Walking Dead Season 4 later today and leading up to the premiere on October 13th. You can catch up on our recent coverage by visiting the following links:
Season 4 Photo Gallery
***Spinoff Series Announced***
Season 4 Interviews: Part 1 | Part 2 | Part 3 | Part 4
Season 4 Trailer | Clip
Season 4 Zombie School Video | Season 4 Banner | Poster
Exclusive Interviews: Andrew Lincoln | Laurie Holden
Season 4 Preview Video


6137896241 who called from (613) 789-6241?
Always calls around the afternoon-evening, even if you don't answer they leave a voice mail in Chinese. If you do answer you're greeted with a messaging system in another language.
Do you have a comment about (613) 789-6241?


Starting a New Business or Organization? Entity Selection is Crucial - Crippen & Co.
During National Entrepreneurship Month, we celebrate those who have courageously stepped out to start their own business ventures. When starting a new business, there are a number of entity choices to select from. Some can easily be ruled out due to the details of your operation, but others will leave you wondering which is right for your venture. State statutes, federal regulations, and income tax laws all come into play when determining the appropriate entity.
1. Sole Proprietorship: A sole proprietorship is owned by one person. The owner may also be the only worker. A sole proprietorship is not a separate legal entity. In a sole proprietorship, the owner receives all profits, bears all losses, and is personally liable for all legal claims that arise. Owners of a sole proprietorship can face legal action from accidents, faulty merchandise, employees, unpaid bills, and other business problems.
3. C-corporation: A C-corporation is a legal entity owned by its stockholders united under a common name. Corporations issue stock and elect a board of directors to manage the company. Shareholders have limited liability for the obligations of the business. With a C-corporation, the corporate income is taxed twice. The corporation distributes its earnings as dividends to stockholders, and those dividends must be declared as personal income on their tax returns.
4. S-corporation: An S-corporation is similar to a C-corporation, but limits the number of stockholders to 100. Many small companies choose this option, because it's a good way to avoid the double taxation of C-corporations, and also provides limited personal liability for the owners. There are some restrictions placed on S-corps, though, which can complicate matters. For example, there can be only one class of stock issued, and the corporation must be domestic.
5. Not-For-Profit Organization: A not-for-profit organization is set up with a specific mission to improve society, such as a museum, charitable foundation, religious organization, research group or trade association. It is not an option for a regular for-profit business. Not-for-profit organizations generally do not pay taxes on their income, cannot sell stock or pay dividends, and have strict requirements imposed on their activities.
6. General Partnership: A general partnership involves two or more owners who make decisions for the business together. Partners share profits, losses, and liability. Although a partnership can be very informal, it is generally considered a legal entity under applicable state law.
In addition, there are multiple sub-entities within some of these six categories. One of these sub-entities may be the correct choice for your new business. To find out for sure, contact us at Crippen & Co. to discuss your options. Entity selection is just one of the Strategic Business Planning services we offer. We can help decide which one is right for your operation, and offer other start-up guidance crucial to the success of a new entrepreneurial venture.


text:
Serviced Office Space Hucknall
Park Road, Nottingham, NG6
This spectacular Victorian building has been expertly modernised and refurbished to create high quality serviced office space. 11 private offices are available to let on flexible terms in to...
Lake View Drive, Nottingham, NG15
As one of the UK's most forward-thinking serviced office providers. Pure Offices own and manage office space in 20 major locations across the UK. Whether you're a start-up or global busin...
Nottingham Road, Nottingham, NG7
Double storey 1980's built offices. First floor space currently available totalling in excess of 4000 sq ft. With open plan space and various sized offices. Flexible terms with serviced an...
Radford Road, Nottingham, NG7
Serviced offices in Nottingham occupy this attractive business centre, close to excellent local transport links and amenities. The building has a range of offices available, as well as offer...
Main Street, Nottingham, NG8
Strelley Hall is an attractive period building set in parkland in the splendidly preserved Strelley village, about 4 miles to the north west of Nottingham. Strelley Hall was originally built...
Brookfield Road, Nottingham, NG5
This centre is a mere four miles from the vibrant heart of Nottingham City, which is very accessible with highly developed local transport links and approximately five miles from Junction 26...
Hucknall Road, Nottingham, NG5
Conventional and Serviced Space Available: This building built in 1880, displays many fine Victorian architectural features. The building occupies over 6,000 sq ft of quality office space a...
Foxhall Road, Nottingham, NG7
Only a mile northwest of the city, this Business Centre, could not be more convenient. Occupying over 15,000 square feet and with secure parking - it poses a spacious and versatile propositi...
Triumph Rd, Nottingham , NG7
ARE you entrepreneurial? DO you work in a person-focused business? If so, we have unique studio SPACE available. Come join our positive creative atmosphere. A uniquely designed 4500 sq f...
/uk/nottinghamshire/nottingham/office-space/park-road-nottingham-ng6-ref-7965.html
/uk/nottinghamshire/nottingham/office-space/lake-view-drive-nottingham-ng15-ref-9724.html
Business park location
/uk/nottinghamshire/nottingham/office-space/nottingham-road-nottingham-ng7-ref-16600.html
/uk/nottinghamshire/nottingham/office-space/radford-road-nottingham-ng7-ref-17802.html
/uk/nottinghamshire-/nottingham/office-space/main-street-nottingham-ng8-ref-22371.html
/uk/nottinghamshire/nottingham/office-space/brookfield-road-nottingham-ng5-ref-8818.html
/uk/nottinghamshire/nottingham/office-space/hucknall-road-nottingham-ng5-ref-6700.html
/uk/nottinghamshire/nottingham/office-space/foxhall-road-nottingham-ng7-ref-3143.html
/uk/nottinghamshire/nottingham-/office-space/triumph-rd-nottingham--ng7-ref-27921.html
Nearest locations to Hucknall
Offices in Nottingham City Centre 5.9 miles
Offices in Nottingham 6.0 miles
Offices in Ilkeston 7.1 miles
Offices in Beeston 7.6 miles
Offices in Carlton 7.8 miles
Offices in Mansfield 7.8 miles
Offices in Stapleford 8.2 miles
Offices in Long Eaton 10.1 miles
Offices in Alfreton 10.5 miles
Hucknall is a town in the county of Nottinghamshire, which is known as the final resting place of Lord Byron as well as the site for the first-ever vertical take-off made by an airplane. Originally called Hucknall Torkard, the town had a thriving market that gave way to industrial development once coal was discovered in the nearby areas. The town has been associated with a number of famous names such as Robin Bailey, Ben Caunt, Jack Hall, Thomas Cecil Howitt and Sam Weller Widdowson, along with Lord Byron, of course. Holgate Comprehensive School of Arts is a major landmark in the area as is the statue "Lost", depicting the mining industry that influenced much of Hucknall.
Doff Portland is one of the biggest companies to have manufacturing facilities and office space in Hucknall. Accountants, financial advisors, clothing merchants, building and construction companies also have offices in the town along with lawyers, landscape architects, jewellery designers and website development companies. Josery Textiles Ltd. and AWNA are amongst the other companies that have their manufacturing facilities in Hucknall.
Hucknall may be a small town but it has its own fair share of entertainment venues with the most prominent being the John Godber Centre, a venue for live-music and the performing arts. Lovelace Theatre is also a popular location, hosting a number of modern and classical theatre productions. Hucknall has quite a few dining options for those looking to eat out in the area. Bella Mia and Moda are two of the most popular Italian eateries in the area while The Tee House is a great place to enjoy some authentic British cooking.
The town has its own railway station along the Robin Hood Line at Hucknall Station, which connects it to Nottingham. The tram-line between Nottingham and Hucknall also stops at nearby Butler's Hill. The A611 passes close to the town while junction 27 connects Hucknall to the M1, allowing bus and coach service to ferry commuters from the town.


Macleod & Co. is a full-service marketing agency located in the North Co. building in Northeast Minneapolis. We have the creative firepower you need (the proof is in the portfolio!), and our strategic approach means that we place a unique emphasis on looking at the whole picture and maximizing your spend. Your success is our success, so every action we take is designed to move your bottom line.
strategy will help you get there.
customers where they live — online.
Your brand is what people connect with.
gold mine — let's put it to work!
creative needs to be compelling.
with. Make sure it's working.
You might be sitting on a content gold mine — let's put it to work!
As it turns out, you can have it all.


text:
Sean Herrmann
Sexual Harassment & Assault
National Origin Discrimination
Veteran Discrimination
Equal Pay & Compensation Discrimination
Wage Theft & Violations
Off the Clock
Independent Contractor Misclassification
Whistleblower & Retaliation
False Claims Act/Qui Tam
Wage and Hour Retaliation
Wrongful Discharge
FMLA & Other Leave
Family & Medical Leave Act
Non-Competition & Trade Secrets
Severance Agreements & Employment Agreements
Greater Expectations: Moms-to-Be Need Better Job Protection
Emily-tSunela	2019-05-10T13:43:10-05:00	May 10th, 2019|
On May 9, 1914, President Woodrow Wilson signed a proclamation carving out the second Sunday in May as Mother's Day. The proclamation called upon Americans to display Old Glory on public buildings and their homes in "a public expression of our love and reverence for the mothers of our country." With all due respect to Mr. Wilson, this patriotic act had better come with brunch, chocolates, and/or flowers if the sons and daughters of our country want to make it to the third Sunday in May.
But even these more modern gestures of thanks and love for the women in our lives are woefully inadequate. Expectant mothers can still be legally fired from their jobs for following routine recommendations from their doctors. New mothers can still be let go for taking off from work immediately following childbirth or later to care for a sick child. The last four years have seen significant progress in these areas, but gaping holes in these safety nets remain.
In 2015, the Supreme Court added some baby teeth to the Pregnancy Discrimination Act ("PDA") in Young v. United Parcel Serv., Inc., 135 S. Ct. 1338 (2015). Four decades earlier, the PDA added new language to Title VII's definitions section. "Title VII" refers to part of the 1964 Civil Rights Act which prohibited employment discrimination based on race, color, religion, sex, or national origin.
The PDA amended Title VII's definition of "sex" to include "pregnancy, childbirth, or related medical conditions." Another clause of the PDA says that "women affected by pregnancy, childbirth, or related medical conditions shall be treated the same for all employment-related purposes . . . as other persons not so affected but similar in their ability or inability to work." 42 U.S.C. § 2000e(k).
This law was straightforward enough in the situation where an employer fires an employee because of her pregnancy or rejects a candidate after a stellar phone screen upon seeing a baby bump in person. These violations still occur with alarming frequency and patronizing excuses that the employers are doing so to protect the women and their babies from the dangers of the job.
Things get harder when the employer fires an employee for inquiring about ways to reduce these dangers, but keep their jobs. Doctors routinely advise pregnant women to avoid or reduce physically demanding tasks, including prolonged standing, long work hours, irregular work schedules, heavy lifting, excessive bending and kneeling, and high physical activity. These requirements are handed out alongside prenatal vitamins and the dreaded list of prohibited meats and cheeses regardless of a particular woman's individual situation. But the law did not clearly protect women who requested these accommodations.
In Young v. UPS, the Court said that these women are protected so long as they can prove: (1) the employer accommodated others "similar in their ability or inability to work"; (2) the employer's policies impose a significant burden on pregnant workers; and (3) the employer's explanation for the different treatment is not sufficiently strong to justify the burden." 135 S. Ct. at 1354. Women can have their cases heard by a jury only if they show that the employer accommodates a large percentage of non-pregnant workers while failing to accommodate a large percentage of pregnant workers. Id.
But the Court simultaneously ruled that pregnant workers are not automatically entitled to the same accommodations given to all other non-pregnant employees. This has led lower courts to apply the PDA less robustly than a full reading of Young would seem to require.
A recent review by A Better Balance—an advocacy group fighting to help working people care for themselves and their families—found that women are losing two-thirds of the cases filed in the wake of Young v. UPS. These cases show lower courts taking a very restrictive view of which non-pregnant employees are "similar in their ability or inability to work" as pregnant employees. If a woman cannot identify a non-pregnant employee with similar work restrictions to the ones her doctor is insisting on for her, courts are allowing employers to deny these accommodations and drive these women out of the workforce.
The difficulty is rooted in the PDA itself. Rather than simply requiring employers to provide reasonable accommodations to pregnant women, the PDA only requires employers to refrain from singling them out for discriminatory treatment. It is only because the Americans with Disabilities Act and Workers' Compensation Acts exist that the PDA can possibly grant job accommodations at all. And it is only where women can point to employees with restrictions similar to their own—and who are benefiting from these other laws—that they are able to secure accommodations that make it possible to keep their jobs throughout their pregnancies and early motherhood.
Just as mothers deserve more than an American flag displayed on the front porch, women need more than the PDA and Young v. UPS provide.
Half the states protect pregnant workers with additional job accommodation requirements. But even in these states, significant issues remain. In North Carolina, for instance, the new rules were put in place by Governor Cooper's 2016 executive order, which means they only protect state employees.
Since 2012, some in Congress have been trying to provide greater federal protections through The Pregnant Workers Fairness Act. If passed, this law would set up the same system for pregnant workers that presently exists for disabled employees all across the country—without the need for pregnant women to locate actual disabled employees with their same restrictions or to then debunk their employers' explanations for the different treatment before they are entitled to job protections and accommodations.
Specifically, The Pregnant Workers Fairness Act would:
Clarify that employers must make reasonable accommodations for workers affected by a known limitation related to pregnancy, childbirth, or related medical conditions.
Require an interactive process between employers and pregnant workers to determine appropriate reasonable accommodations, similar to the Americans with Disabilities Act.
Provide an exemption for businesses if an accommodation imposes an undue hardship on an employer.
Protect pregnant workers from retaliation, coercion, intimidation, threats or interference if they request or use an accommodation.
Apply to employers with 15 or more employees and provide protections for both job applicants and employees.
While even these protections would fall short of protecting women in small businesses or addressing the need for paid leave, they would be a big step in the right direction and are deserving of bipartisan support.
Instead of a Star-Spangled salute, or even brunch, flowers, and chocolates this year, we owe our expectant mothers job security and the ability to continue providing for their families.
Reach out for a consultation today.
We had the opportunity to work with Kevin, and he was amazing! He got our case resolved quickly and to our advantage. I would highly recommend him and his firm.
- G.B. M.
I chose Sean over other options because he took the time to make me feel at ease and ensure that he fully understood all of the relevant information. I would strongly recommend Sean for any employment related issues.
- Veronica
I was very pleased with Kevin as my attorney. He took the time to hear my side of the story and really explained all my options completely. He stood by any decisions that I made and acted accordingly. I will definitely consider him in the future if in need of an attorney.
- Darrin
I highly recommended working with Herrmann & Murphy. I personally worked with Kevin Murphy from the beginning of my process until the ending the level of professionalism never wavered. He comes with a wealth of knowledge dealing with employment issues.
- Sabrina M.
Kevin Murphy was excellent in handling my case. Very professional throughout the entire process. Extremely knowledgeable and displayed superb negotiating skills. He explained to me each step of the case as it proceed in terms that everyone could understand. When you need a lawyer, you want Kevin Murphy in your corner!
- Roger
Sean was great. I was being retaliated against and Sean helped me prove it with his legal advice. He is honest, knowledgeable and has integrity. If I ever have any problems again, Sean is my first choice.
I had a wonderful experience working with Kevin Murphy. He is very professional and detailed in his work. It was a pleasure working with this firm.
-Katie H.
Kevin was very responsive to my needs. Always keep in touch with me and explaining what the process was. His advice was invaluable and very helpful. I would recommend him to anyone who needed an attorney for my issue.
Kevin was more than ideal to have on our side. We were impressed with his organization, determination and knowledge of the matter at hand. His constant updates and communication were very much appreciated. Mr. Murphy comes highly recommended by our family.
-C. Carpenter
Kevin was a great lawyer! I had a very easy experience working with him. He made a stressful situation much easier and was able to negotiate a great outcome. He was incredibly professional and was great at communicating all steps of the process to me. Highly recommend!
- Maggie
10.0Kevin Patrick Murphy
10.0Sean Franklin Herrmann
Severance Agreements &
Qui Tam/False Claims
© 2019 Herrmann & Murphy, PLLC. ALL RIGHTS RESERVED. TERMS OF USE AND DISCLAIMER PRIVACY


Is imreoir leadóige gairmiúla as An Eilvéis í Timea Bacsinszky. Rugadh í ar an 8 Meitheamh, 1989. Faoi láthair tá sí rangaithe ag uimhir 660.
Tugadh an leathanach seo cothrom le dáta an 16 Eanáir 2019 ag 19:52.


text:
Follow From Front Comic Strips - Page 4
490 Results for Follow From Front
View 31 - 40 results for follow from front comic strips. Discover the best "Follow From Front" comics from Dilbert.com.
Tags Dilbert, dinosaurs, theory, giant meteor, earth, larry
Bob and Dawn the Dinosaurs sit on the floor in front of Dilbert's chair. Dilbert says, ". . . So the theory that dinosaurs were destroyed when a giant meteor collided with earth . . ." Bob says, ". . . Was highly exaggerated." A dinosaur points at another dinosaur who lies underneath a meteor and says, "Ha ha, Larry! Ha ha!" Another animal says, "Nice catch." From underneath the meteor, Larry says, "Ouch!"
Thursday July 27, 1989
Tags Dilbert, Dogbert, design, barbecues, electricity
Dilbert sits at his desk with drafting tools in front of him. Dilbert looks at a piece of paper and says, "This design could change the way the world barbecues. No more struggling with charcoal." Dilbert shows the paper to Dogbert and says, "This schematic shows how an electrically induced coil-like medium can heat food without charcoal or lighter fluid!" Dilbert continues, "I call it the Max-10 Energy Transfer Model." Dogbert asks, "Did the name 'Electric Stove' occur to you at any time?"
Tags Dogbert, Dilbert, shower, egg, lassie, rin tin tin, benji, big, furry
Dogbert stands on a stool and looks in the bathroom mirror. He asks, "Do you think I look more like Rin Tin Tin or Lassie? Maybe Benji?" Dilbert stands in front of the shower with a towel around his waist. Dilbert replies, "I dunno . . . Maybe a big furry egg." Dogbert says, "With that little inspiration boost, I believe I am ready to attack the new day."
Tags Dilbert, newspaper, thief, machine, money, eating
Dilbert puts a coin in a newspaper machine and thinks, "Sometimes I get this wicked urge to take two newspapers and only pay for one." Dilbert looks behind him and thinks, "What's the worst that can happen? Besides, this machine ate my money last time." Dilbert looks at a newspaper. On the front page is a picture of him stealing the newspaper and the headline says, "Thief!"
Tags Dilbert, Dogbert, book, walking, dog, picking up, Women, baby, single male, overrated
Dilbert looks in a book and says to Dogbert, "This book says the best time to pick up women is while walking a dog." Dogbert says, "Let's try it." Dilbert holds a leash that is wrapped around Dogbert's stomach. Dogbert walks in front of Dilbert yelling, "Yo! Baby! Whoa whoa! Shake it, don't break it! Come and get your single male!!" Dilbert says, "I think this method is overrated." Dogbert yells, "Form one line! No pushing!"
Tags Dilbert, prototype, six months, transform, pocket, lint, parsley substitute, technology driven
Dilbert stands in front of a man's desk holding a gadget. The man asks, "So, Dilbert, this is the prototype you've been working on for the last six months?" Dilbert replies, "Yes, sir. I'm proud to say that this baby can transform worthless pocket lint into a valuable parsley substitute!" The man says, "Well, this looks absolutely brilliant and completely unmarketable." Dilbert says, "Thanks, I'm technology driven."
Tags Dilbert, Women, party, act, naturally, scrutiny
Dilbert stands in front of the dresser mirror adjusting his tie and Dogbert sits on the bed watching him. Dogbert says, "The best way to impress women at the party is to just act naturally." Dilbert walks though a party thinking, "Act naturally, act naturally." Back at home, Dilbert says, "Logically, it is impossible to 'act' naturally." Dogbert says, "Most great advice doesn't hold under scrutiny."
Tags Dilbert, Dogbert, tombstone, write, cremated, stuffed, cheaper, arm chair
Dogbert sits in a chair and Dilbert stands in front of him. Dilbert asks, "If I died tomorrow, what would you write on my tombstone?" Dogbert replies, "I always assumed there would be no tombstone." Dilbert says, "Ah . . . You would have me cremated." Dogbert replies, "Or stuffed, whichever is cheaper."
Tags Dilbert, Dogbert, informal, white house, gorby, gumby, community
Dilbert sits in his chair and Dogbert sits on Dilbert's legs. Dogbert says, "I'm enjoying the new informal approach at the White House." Dogbert continues, "I just hope it doesn't embarrass us in the international community." A White House aide stands in front of the President's desk next to Gumby. The President says, "Doggone it, I told you to set up a meeting with GORBY!" The aide thinks, "What's a Gorby?"
Tags Dilbert, woman, dating, love, unattractive, blob, friends
Dilbert stands in front of a woman's desk. The woman says, "Well . . . I would date you . . ." The woman continues, "But frankly I think of you as a boring and unattractive blob of organic matter . . ." The woman concludes, "So let's just be friends."


JEE Main, NATA – Apart from the engineering courses, students choose architecture, every year thousands of students pursue their career. In India, School of Planning and Architecture in New Delhi, the Indian Institute of Technology, Kharagpur (IIT-Kharagpur) and IIT-Kanpur are some of the well-known architecture colleges. Though every college has their admission criteria, to get a chance in IITs, the students have to appear in JEE (Main), JEE (Advance). Take a look at the list of architecture courses, colleges and entrance examinations in India.
To architecture course in various institutes in the country, the entrance examination is conducted every year for admission. On their drawing and observation skills, sense of proportion, aesthetic sensitivity and critical thinking ability, it measures the applicant's aptitude for architecture and focuses. For two years from the year of appearing, the scorecard from this test is valid.
General Aptitude (MCQ) bearing two marks each (40×2= 80 marks).
From January 6 to 20, 2019, the first examination of JEE Main will be conducted and from April 6 to 20, 2019, the second examination.
To be eligible for JEE Main or JEE Advanced, a score of at least 75 per cent in Class 12 board exams or a place among the top 20 percentiles of the Boards is required. This has been lowered to 65 per cent for candidates of the SC and ST categories.
For seats in different engineering, architecture institutes, COMEDK UGET entrance exam is an online entrance test conducted all over the country. To ensure merit-based admissions through a single common entrance test, the entrance test will be held in a regular mode.
Result: First or second week of June.
For aspirants who want admission to the architecture programmes in IIT Roorkee and IIT Kharagpur, JEE Advanced AAT is the entrance test.
To foreign nationals or non-resident Indians for admissions to architecture/ engineering/ management programmes in reputed institutions in India, Direct Admission of Students Abroad (DASA), offered by the Union HRD ministry is a scheme provided. By their Scholastic Aptitude Test (SAT score), the students got admissions.


text:
Chester Ray Benjamin (January 23, 1923 – April 20, 2002) was an American mycologist. His research was focused on the taxonomy of fungal molds belonging to the orders Eurotiales and Mucorales. Born and raised in Ohio, Benjamin received his undergraduate education from Mount Union College in Alliance after serving in the Navy for four years during World War II. Benjamin earned his Doctoral degree (dissertation title: "Ascocarps of Aspergillus and Penicillium") in 1955 from the University of Iowa under the tutelage of George Willard Martin.

In 1960 Benjamin started an 11-year position as principal mycologist in the Crops Protection Research Branch of the Agricultural Research Service in Beltsville. In 1967, Benjamin was the president of the Mycological Society of America. From 1971 until his retirement, he held various positions in the Agriculture Department. Benjamin died of a heart attack at his home in Silver Spring, Maryland at the age of 79. The genus Benjaminia was named in his honor.

See also
List of mycologists

References

American mycologists
1923 births
2002 deaths
University of Mount Union alumni
University of Iowa alumni
People from Silver Spring, Maryland
United States Navy personnel of World War II


text:
Q: Invalid operands to binary expression on TGridOptions I have recently upgraded from C++ Builder XE8 to Rad Studio 10 Seattle.  I am trying to use the new Clang compiler but I am running into an issue.
On a custom grid class I have the following line of code:
__property Options = {default=TGridOption::AlternatingRowBackground << TGridOption::RowSelect};

Which causes the following error from the compiler:
[CLANG Error] FmGridU.h(57): invalid operands to binary expression ('Fmx::Grid::TGridOption' and 'Fmx::Grid::TGridOption')
From what I have read in other questions, I need to do something like implement my own << operator.  However, I am not exactly sure how I would go about doing this.  From my understanding, the current code is the standard way to work with control options.
What is the difference with the new Clang compiler that causes it to thrown an error where the Classic Boreland compiler does not?  How can I implement the << operator to allow me to set the options property?
Edit:
I have corrected my syntax as per Remy's suggestion.
__property Options = {default = TGridOptions() << TGridOption::AlternatingRowBackground << TGridOption::RowSelect};

However, now I get the following error:
'expression is not an integral constant expression'
According to this question the answer was to put the code inside of a function.  However, since I am declaring this property in a header file, I am not sure how to do that.  Is there something else I am missing?

A: That is not valid syntax, in either the classic compiler or the new CLang compiler.  Options is a TGridOptions, which is a Set<> of TGridOption values (ie: typedef System::Set<TGridOption, TGridOption::AlternatingRowBackground, TGridOption::HeaderClick> TGridOptions;).  You need to construct an actual TGridOptions object before you can assign any values to it, eg:
TGridOptions MyOptions = TGridOptions() << TGridOption::AlternatingRowBackground << TGridOption::RowSelect;

However, you cannot create a Set<> object inside of a property declaration.
What you can do, though, is specify a numeric constant that represents the binary content of a Set<> object.  In this case, for a TGridOptions set, TGridOption::AlternatingRowBackground is located at bit 0 and TGridOption::RowSelect is located at bit 7, thus the numeric value of the set that contains both TGridOption::AlternatingRowBackground and TGridOption::RowSelect enabled is binary 10000001, hex 0x81, decimal 129, thus you can declare the property like this:
__property Options = {default = 0x81};

__property Options = {default = 129};

This is easier to handle in Delphi than in C++, as Delphi lets you specify the actual set (which the Delphi compiler translates to a numeric constant when generating a C++ .HPP file):
property Options default [TGridOption.AlternatingRowBackground, TGridOption.RowSelect];

In either case, as with any other property, make sure that you are actually assigning the same TGridOptions default value in your grid's constructor to match the property declaration, or else the property will not stream to/from a DFM/FMX resource correctly.  In this case, you can use a real TGridOptions object to assign the property value:
__fastcall TMyGrid::TMyGrid(TComponent *AOwner)
    : public TCustomGrid(AOwner)
{
    Options = TGridOptions() << TGridOption::AlternatingRowBackground << TGridOption::RowSelect;
}


Just as each grain of sand makes a beach, each holiday leaves an imprint on your life. The secluded and sheltered beach of Copthorne Orchid Hotel Penang will make that difference in your holiday.
Here, you can enjoy the luxuries of sand and shade, but what you will value most is the privacy, tranquility and safe sanctuary that we offer you in our natural environment.
Guests of the hotel can relax here away from hustling petty traders and the prying eyes of the public. It is the perfect getaway from city life while the beach is also located a good distance away from the main road, therefore, making it free from disturbances and noise.
Nearby the hotel is a major shopping complex and bank. A clinic and food court can be found within walking distance from the hotel.


text:
Filmografia parziale
I ragazzi del sabato sera (Welcome Back, Kotter) - serie TV, 95 episodi (1975-1979)
Lewis & Clark - serie TV, 13 episodi (1981-1982)
 La signora in giallo (Murder, She Wrote) - Serie TV, episodio 1x02 (1984)

Poker
Ha fatto la sua prima apparizione alle World Series of Poker nel 1978. Nel luglio del 2004, si classifica in terza posizione in una tappa del World Poker Tour, vincendo poco più di $250,000. Nel 2005 è inoltre arrivato secondo nel torneo $5000 Limit Hold 'Em delle WSOP, vincendo $222,515.

Kaplan è stato il principale commentatore televisivo delle WSOP del 1997 e del 2002. Nel 2007, ha vinto l'episodio "Queens and Kings" del Poker After Dark, battendo in heads-up Kristy Gazes, e avendo la meglio su giocatori del calibro di Howard Lederer, Ali Nejad, Vanessa Rousso e Annie Duke. Alle WSOP del 2007 è arrivato nono nel torneo $50,000 World Championship H.O.R.S.E, vincendo $131,424. Nel 2008 vince nuovamente l'episodio "Cowboys" del Poker After Dark contro Chris Ferguson, Andy Bloch, Chau Giang, Hoyt Corkins e Doyle Brunson.

Al 2015, il totale delle sue vincite nei tornei live supera i $1,991,248, di cui $539,159 vinti alle WSOP.

Note

Altri progetti

Collegamenti esterni


GitHub - conda-forge/r-survival-feedstock: A conda-smithy repository for r-survival.
A conda-smithy repository for r-survival.
Want to be notified of new releases in conda-forge/r-survival-feedstock?
Summary: Contains the core survival analysis routines, including definition of Surv objects, Kaplan-Meier and Aalen-Johansen (multi-state) curves, Cox models, and parametric accelerated failure time models.
If you would like to improve the r-survival recipe or build a new package version, please fork this repository and submit a PR. Upon submission, your changes will be run on the appropriate platforms to give the reviewer an opportunity to confirm that the changes result in a successful build. Once merged, the recipe will be re-built and uploaded automatically to the conda-forge channel, whereupon the built conda packages will be available for everybody to install and use from the conda-forge channel. Note that all branches in the conda-forge/r-survival-feedstock are immediately built and any created packages are uploaded, so PRs should be based on branches in forks and branches in the main repository should only be used to build distinct package versions.


The currency of Egypt. It was introduced in 1834, replacing the piastre, which continued to circulate at a value of 1% of a pound. From 1885 to 1914, the pound was unofficially pegged to gold. Afterward, it was officially pegged to the British pound until 1962, when the peg switched to the U.S. dollar at various rates. The Egyptian pound became a floating currency in 1989.
Majid Al Futtaim's latest investment comes after the recent announcement of its five-year investment plan worth 18 billion Egyptian pounds.
He added that since Monday banks have witnessed a new phenomenon: customers selling parts of their deposits and saving certificates in foreign currencies, switching them to Egyptian pounds, and then buying the pound saving certificates with a 16% or 20% interest rate that the banks offered recently.
Cairo: The Egyptian pound hit a record low against the dollar yesterday as worried Egyptians shifted their money out of local currency after the central bank introduced a new currency regime to protect foreign reserves.
Sheikh Saleh bin Abdullah Kamel, President of the Saudi Commerce and Industry Chamber and Chairman of Jeddah Commerce and Industry Chamber, disclosed that a Saudi developmental bank with a capital of one billion Egyptian pound would be established in Egypt at an initiative of businessmen to help alleviate the current economic situation in Egypt following the recent incidents.
then that will continue to breed uncertainty, and the Egyptian pound will reflect that.
People walk past a major branch of HSBC at the financial Central district in Hong Kong - Reuters LONDON - 12 December 2017: HSBC economic experts said the tough measures taken by the Central Bank of Egypt (CBE) and the Egyptian government over the past few months before November 3, 2016 when the decision of liberalizing the Egyptian pound was issued have rescued the Egyptian economy, according to a report published by "Risk Net" financial risk management website.
He added that this step comes complementary to floating the Egyptian pound.
The value of the Egyptian pound took a beating after the central bank purchased dollars.
The Egyptian pound, subject to a managed float, gained 0.
Summary: The Egyptian pound had been falling steadily since political protests broke out on Jan 25.
Dubai Bonyan for Development and Trade yesterday said that it has finalised arrangements for an 185 million Egyptian pound (Dh123 million) syndicated loan to finalise the completion of Designopolis, the first furniture and home accessories mega mall in the Middle East and North Africa.
A state of uncertainty over the timing of the devaluation of Egyptian pound by the Central Bank of Egypt (CBE) pervaded the market Sunday, following the meeting held between President Abdel Fattah Al-Sisi, CBE governor Tarek Amer, and a number of senior state officials.


text:
Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells
Carlos L. Aparicio, Francois Berthiaume, Charlie C. Chang, Martin L. Yarmush
School of Engineering, Biomedical Engineering
Recent in vivo studies suggest that tumor necrosis factor-α (TNF-α) is involved in the development of the thymus. We postulated that this inflammatory mediator could regulate the influx of progenitor T cells into the thymus. Using an in vitro static adhesion system, we found that TNFα increases the adhesion of a murine progenitor T cell line (FTF1) to a bovine aortic endothelial cell line (1F8), human umbilical vein endothelial (HUVE) cells, and a murine arterial endothelial (MAE) cell line. TNF-α treatment of the 1F8 cells resulted in a time- and dose-dependent increase in the adherence of FTF1 cells. Adherence increased during the first 6 hr of treatment with TNF-α concentrations ranging from 10-11 to 10-9 M. Maximal adherence (6 hr treatment with 10-10 M of TNF-α) was approximately 4.5-fold larger than that of untreated monolayers. A slow decrease in adherence, down to approximately 2-fold at 48 hr, was observed beyond 12 hr o TNF-α treatment; in contrast, removal of TNF-α after 6 hr of continued stimulation caused the adherence to return to pre-stimulation levels within 24-30 hr. Adhesion of FTF1 cells to TNF-α-treated 1F8 cells was almost completely blocked by a monoclonal antibody against murine CD49d (very late antigen-4) expressed on FTF1 cells. TNF-α-induced adhesion of FTF1 cells to MAE cells was also blocked by monoclonal antibodies against murine CD49d and CD106 (vascular cell adhesion molecule-1). These results support the notion that local secretion of TNF-α could modulate the dynamics of adhesion of progenitor T cells to the thymic endothelium.
FTF1 cell
cell cell adhesion
endothelial cell
projenitor T cell
tumor necrosis factor-α
Fingerprint Dive into the research topics of 'Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells'. Together they form a unique fingerprint.
Stem Cells Medicine & Life Sciences
Tumor Necrosis Factor-alpha Medicine & Life Sciences
Thymus Gland Medicine & Life Sciences
Cell Adhesion Medicine & Life Sciences
Monoclonal Antibodies Medicine & Life Sciences
Aparicio, C. L., Berthiaume, F., Chang, C. C., & Yarmush, M. L. (1996). Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells. Molecular Immunology, 33(7-8), 671-680. https://doi.org/10.1016/0161-5890(96)00013-2
Aparicio, Carlos L. ; Berthiaume, Francois ; Chang, Charlie C. ; Yarmush, Martin L. / Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells. In: Molecular Immunology. 1996 ; Vol. 33, No. 7-8. pp. 671-680.
@article{9a7948fbf1c74c82840d59ec750fead9,
title = "Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells",
abstract = "Recent in vivo studies suggest that tumor necrosis factor-α (TNF-α) is involved in the development of the thymus. We postulated that this inflammatory mediator could regulate the influx of progenitor T cells into the thymus. Using an in vitro static adhesion system, we found that TNFα increases the adhesion of a murine progenitor T cell line (FTF1) to a bovine aortic endothelial cell line (1F8), human umbilical vein endothelial (HUVE) cells, and a murine arterial endothelial (MAE) cell line. TNF-α treatment of the 1F8 cells resulted in a time- and dose-dependent increase in the adherence of FTF1 cells. Adherence increased during the first 6 hr of treatment with TNF-α concentrations ranging from 10-11 to 10-9 M. Maximal adherence (6 hr treatment with 10-10 M of TNF-α) was approximately 4.5-fold larger than that of untreated monolayers. A slow decrease in adherence, down to approximately 2-fold at 48 hr, was observed beyond 12 hr o TNF-α treatment; in contrast, removal of TNF-α after 6 hr of continued stimulation caused the adherence to return to pre-stimulation levels within 24-30 hr. Adhesion of FTF1 cells to TNF-α-treated 1F8 cells was almost completely blocked by a monoclonal antibody against murine CD49d (very late antigen-4) expressed on FTF1 cells. TNF-α-induced adhesion of FTF1 cells to MAE cells was also blocked by monoclonal antibodies against murine CD49d and CD106 (vascular cell adhesion molecule-1). These results support the notion that local secretion of TNF-α could modulate the dynamics of adhesion of progenitor T cells to the thymic endothelium.",
keywords = "FTF1 cell, cell cell adhesion, endothelial cell, interleukin-1, projenitor T cell, tumor necrosis factor-α",
author = "Aparicio, {Carlos L.} and Francois Berthiaume and Chang, {Charlie C.} and Yarmush, {Martin L.}",
journal = "Molecular Immunology",
Aparicio, CL, Berthiaume, F, Chang, CC & Yarmush, ML 1996, 'Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells', Molecular Immunology, vol. 33, no. 7-8, pp. 671-680. https://doi.org/10.1016/0161-5890(96)00013-2
Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells. / Aparicio, Carlos L.; Berthiaume, Francois; Chang, Charlie C.; Yarmush, Martin L.
In: Molecular Immunology, Vol. 33, No. 7-8, 01.01.1996, p. 671-680.
T1 - Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells
AU - Aparicio, Carlos L.
AU - Berthiaume, Francois
AU - Chang, Charlie C.
AU - Yarmush, Martin L.
N2 - Recent in vivo studies suggest that tumor necrosis factor-α (TNF-α) is involved in the development of the thymus. We postulated that this inflammatory mediator could regulate the influx of progenitor T cells into the thymus. Using an in vitro static adhesion system, we found that TNFα increases the adhesion of a murine progenitor T cell line (FTF1) to a bovine aortic endothelial cell line (1F8), human umbilical vein endothelial (HUVE) cells, and a murine arterial endothelial (MAE) cell line. TNF-α treatment of the 1F8 cells resulted in a time- and dose-dependent increase in the adherence of FTF1 cells. Adherence increased during the first 6 hr of treatment with TNF-α concentrations ranging from 10-11 to 10-9 M. Maximal adherence (6 hr treatment with 10-10 M of TNF-α) was approximately 4.5-fold larger than that of untreated monolayers. A slow decrease in adherence, down to approximately 2-fold at 48 hr, was observed beyond 12 hr o TNF-α treatment; in contrast, removal of TNF-α after 6 hr of continued stimulation caused the adherence to return to pre-stimulation levels within 24-30 hr. Adhesion of FTF1 cells to TNF-α-treated 1F8 cells was almost completely blocked by a monoclonal antibody against murine CD49d (very late antigen-4) expressed on FTF1 cells. TNF-α-induced adhesion of FTF1 cells to MAE cells was also blocked by monoclonal antibodies against murine CD49d and CD106 (vascular cell adhesion molecule-1). These results support the notion that local secretion of TNF-α could modulate the dynamics of adhesion of progenitor T cells to the thymic endothelium.
AB - Recent in vivo studies suggest that tumor necrosis factor-α (TNF-α) is involved in the development of the thymus. We postulated that this inflammatory mediator could regulate the influx of progenitor T cells into the thymus. Using an in vitro static adhesion system, we found that TNFα increases the adhesion of a murine progenitor T cell line (FTF1) to a bovine aortic endothelial cell line (1F8), human umbilical vein endothelial (HUVE) cells, and a murine arterial endothelial (MAE) cell line. TNF-α treatment of the 1F8 cells resulted in a time- and dose-dependent increase in the adherence of FTF1 cells. Adherence increased during the first 6 hr of treatment with TNF-α concentrations ranging from 10-11 to 10-9 M. Maximal adherence (6 hr treatment with 10-10 M of TNF-α) was approximately 4.5-fold larger than that of untreated monolayers. A slow decrease in adherence, down to approximately 2-fold at 48 hr, was observed beyond 12 hr o TNF-α treatment; in contrast, removal of TNF-α after 6 hr of continued stimulation caused the adherence to return to pre-stimulation levels within 24-30 hr. Adhesion of FTF1 cells to TNF-α-treated 1F8 cells was almost completely blocked by a monoclonal antibody against murine CD49d (very late antigen-4) expressed on FTF1 cells. TNF-α-induced adhesion of FTF1 cells to MAE cells was also blocked by monoclonal antibodies against murine CD49d and CD106 (vascular cell adhesion molecule-1). These results support the notion that local secretion of TNF-α could modulate the dynamics of adhesion of progenitor T cells to the thymic endothelium.
KW - FTF1 cell
KW - cell cell adhesion
KW - endothelial cell
KW - interleukin-1
KW - projenitor T cell
KW - tumor necrosis factor-α
JO - Molecular Immunology
JF - Molecular Immunology
Aparicio CL, Berthiaume F, Chang CC, Yarmush ML. Tumor necrosis factor-alpha (TNF-α) induces a reversible, time- and dose-dependent adhesion of progenitor T cells to endothelial cells. Molecular Immunology. 1996 Jan 1;33(7-8):671-680. https://doi.org/10.1016/0161-5890(96)00013-2


Optimal conditions for the production of sulfated polysaccharide by marine microalga Gyrodinium impudicum strain KG03.
Yim JH, Kim SJ, Ahn SH, Lee HK. Optimal conditions for the production of sulfated polysaccharide by marine microalga Gyrodinium impudicum strain KG03. Biomol Eng. 2003;20(4-6):273-80.
A marine microalga Gyrodinium impudicum strain KG03 produced sulfated exopolysaccharide designated as p-KG03, which showed a strong antiviral activity against encephalomyocarditis virus (EMCV). To optimize culture conditions for the production of p-KG03, mineral salts, vitamins, plant growth hormones, temperature, pH and light conditions were examined. From this study, M-KG03 medium for the maximum production of p-KG03 was suggested as follows; NH(4)Cl 75 microM, NaH(3)PO(4) 200 microM, NaHCO(3) 50 microM, Na(2)SO(4) 10 microM, FeCl(2) x 6H(2)O 10 microM, MnCl(2) x 4H(2)O 0. 1 microM, vitamin B(12) 0. 75 microg, naphthalene acetic acid (NAA) 7. 5 microg and myo-inositol 200 mg per liter of aged sea water. The optimal temperature and pH were 22. 5 degrees C and 8. 0, respectively. The optimal light conditions of intensity and period were 150 microE m(-2) s(-1) and 16:8 h light:dark cycle. Finally, the cell growth and p-KG03 production were measured in one liter of M-KG03 medium with 1% CO(2) and 50 ml min(-1) of airflow using two liters airlift balloon type photobioreactor (ABTPR). At these optimal conditions, p-KG03 production and cell growth were 134. 6+/-5. 9 mg l(-1) and 123,076+/-1,597 cells ml(-1), respectively, representing a 7. 7 and 5. 1 times compared with f/2 medium with Erlenmeyer flask culture (p-KG03 production 17. 5+/-1. 3 mg l(-1) and cell growth 24,311+/-1,291 cells ml(-1)).