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written by Chip
Heading out to Santa Barbara for the first tournament of the season, we were seeded 3rd in our pool and 11th overall. After weeks of practice, the team was excited to see how we would stack against our competition.
The first game was against UCLA BLU, a team we played several times during the pre-season and seeded 3rd overall. After trading off points, we fell behind and BLU was able to take the win 9-14.
After the first game we had a bye before playing Colorado, who was seeded 2nd in the pool. We immediately came out strong and held our lead at half. With great plays on defense and flow on offense, we took the game 15-10. Our next game was against Cal Poly SLO where we were able to pull out a 15-6 victory, putting us 2nd in our pool.
Our fourth game was a crossover game where we played UC Santa Cruz. Pumped and ready to play, the theme of our weekend - hustle - was evident throughout this game. Rookie handlers stepped up to make great plays and cutters were throwing upline, trusting themselves with the disc instead of relying on handler movement. We were able to take a big lead, giving UCSC little opportunity to come back. We ended the game with a 15-10 win, putting us in the Championship Bracket for the first time in years.
Sunday's games started out with a match-up against Sonoma State. Sonoma played a fast-paced offense, dominated by three veteran handlers and cutters. Immediately they took half and took the game 4-15. Sonoma ended up winning SB Invite, dominating the competition.
Our next game was against Humboldt State, an opponent we played twice last year. Playing for a chance at 5th place, we suffered a tough 10-14 loss.
The Psychos ended the tournament tied for 7th and with a stellar performance, Santa Barbara was a great learning experience for us. With a couple weeks to train before Pres Day Invite, the Psychos are stoked to see how much damage we can do this season.


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"Well, here's what I think …"
Technology has been a great workplace equalizer, allowing everyone to perform tasks that once required specialized skills, and flattening organizational charts as companies reduce headcount. But added responsibility doesn't automatically equate knowledge or expertise.
I mention that because a side effect of that change is a growing misconception that anyone capable of forming an opinion is automatically entitled to the knowledge and expertise needed to make that opinion valid or meaningful. As a result, we're shifting to a business world in which all opinions are granted the same weight, whether they come from someone with twenty years of experience or a newly minted title.
A wise boss of mine said it was the biggest and most dangerous change he'd seen over his career. He called it "the death of apprenticeship," explaining that not all that long ago, no matter what field someone worked in, there was a process through which the companies would impart knowledge and expertise — typically, by having them work with others who had developed that expertise. Only after a suitable amount of time in that "apprenticeship" would they be placed in positions in which they'd have decision-making duties. The underlying premise was that opinions of people with less experience and exposure to the business world were intrinsically less valuable.
As companies rush to shrink headcount and flatten the organizational chart, those systemic opportunities to develop knowledge have disappeared. The result is that key decisions are being made by people who haven't had the chance to develop the wisdom that grows alongside experience. It isn't that they don't care, it's that they don't know enough to recognize what they don't know. That's dangerous.
I'm not saying that there aren't highly talented people who have acquired knowledge and standing in a significantly shorter time. I've worked with many people who had sharper skills and better judgment in their 20s than their colleagues who were a lot closer to retirement age. But those people have been the exceptions, not the rule.
As soon as someone making decisions about a project starts using statements like "Well, I think …" or "my friends think …" or "the people I've been talking to think …", I know the project is doomed to fail — unless the people being cited are perfect representatives of the customers, prospects, or other audiences being targeted. A common example I've seen over the years is making advertising decisions based upon the decision-maker's personal consumption of media instead of what research shows the company's actual prospects prefer.
I don't place the blame on those individuals. They've been put in positions of authority, and they have every right to exercise that authority as they see fit. It's a matter of management. When company leaders and managers don't have enough knowledge about the functional areas to recognize the amount of skill decisions in those areas require, they're setting their own companies up for waste and missteps at best, and eventual failure at worst.
I believe the key for company leaders is to look beyond headcount to individual skills. If you're flattening the organizational chart in an effort to cut costs, take part of what you would have paid to supervisory staff and invest it in education for the people who have been given new roles. If you're going to place someone in a role in which they'll be responsible for significant decisions, help them acquire the knowledge they need to make those decisions with confidence. Otherwise, there's a good chance that you'll just confirm the Peter Principle.
If you're the person who has been promoted into a role that may be stretching your competency, don't be afraid to admit it. Look for resources that can reinforce areas in which you may not be as strong as you'd like. That may involve classes at a local college, attending professional conferences or workshops, or even finding a mentor within your company or at a company that doesn't compete with yours. If you haven't been placed in an apprentice role, create one for yourself.
A promotion can be an intoxicating ego boost, but don't let it cloud your judgment or give you inflated self-confidence. The smartest, most successful business leaders I've known are acutely aware of what they don't know, and that was central to their success.

redpajama_set_name: RedPajamaCommonCrawl


Everyone needs to be able to have dental care. However, some people need special facilities or services to have this care provided. People with physical disabilities may have problems getting into the surgery or even into the dental chair. People with learning disabilities may become overanxious at the thought of going to the dentist or may need extra reassurance. People suffering from severe medical problems may need extra precautions or care. Dental teams are able to take account of all these things when providing dental care.
Many dentists will happily treat people with special dental needs in their surgery. However, some people find it hard to get to the surgery and so other arrangements may be made by the practice: for example, home visits and special health centres. Some people need a specialist service. The local Community Dental Service or some health centres will also help people needing specialist care and may be able to offer possible treatment alternatives, such as sedation or general anaesthetic.
How do I arrange specialist care?
Usually the patient's dentist or doctor is responsible for referring them to the clinic best suited to their needs. Normally, the dentist or doctor will write a referral letter and send it, with any hospital letters and x-rays, to give the dental team an idea of the patient's dental history.
What do the dental team need to know?
The dental team will need to know the patient's medical history and about any medicines they are taking. This includes any inhalers and regularly prescribed medicines from the doctor. The dental team will also need to know the name of the family doctor and hospital consultant, and about any recent operations and allergies the patient may have.
It is also helpful if the dental team know about any concerns or anxieties the patient has, so that they can help to make the patient feel at ease. This information can be given by the patient's parents or care giver. However, some patients do prefer to communicate directly with the dentist. Some patients may have other special needs: for example, the help of an interpreter or translator, or to have a guide dog. Dentists are prepared for working in these situations.
Some patients prefer to be seen at certain times of the day depending on their needs. For instance, evening appointments may not be suitable for patients who tire easily or may spend the day worrying. Some patients rely heavily on routine and may need regular appointments at the same time.
Practices should offer facilities for wheelchair users, including access to the practice, and ground-floor surgeries. If wheelchair access is particularly important, contact the surgery and ask if this is something they are prepared for. Some clinics have specially adapted surgeries for patients with mobility problems.
What about children's dental treatment?
Children with learning disabilities or other medical conditions may be referred to a specialist dental service by their doctor or dental team. It is important to take children to see the dentist at an early age. A low-sugar diet is also important, as they may be more likely to have tooth decay due to having problems with brushing and through taking medication. Make sure they have fizzy drinks, and sugary foods and drinks, just at mealtimes, and in moderation.
Why is medication an issue?
Many patients with disabilities have to rely on medication to keep their condition under control. It is therefore important to ask the doctor to prescribe sugar-free medicines, especially if they are syrups.
It is important to tell the dental team about any medication that the patient is taking, in case the treatment is affected or the team need to take any extra precautions.
Is there anything to make brushing easier?
For some people, moving their arms or hands can be a problem, which makes effective cleaning difficult. It is important to reach all the areas of the mouth to clean effectively. A toothbrush with a small to medium head size with soft to medium bristles is usually recommended. There are special handgrips and other adaptations which can be fitted to manual toothbrushes to make them easier to hold.
Intravenous sedation (an injection) is an effective way of treating most nervous patients. The drugs given can relax and calm the patient, so treatment can be carried out with the dental team and patient still able to talk to each other. There are certain things that affect a patient's suitability for this type of sedation. These include weight, age and medical condition. This would all be discussed during the consultation. Sometimes the patient would need to be referred to a specialist clinic for this treatment.
Relative Analgesia (RA) can also help patients get through their treatment more easily. Here, nitrous oxide and oxygen are breathed in through a nosepiece. It is the safest and simplest form of sedation and is often the one most suitable for both children and people with special needs. However, this is not appropriate for everyone - especially people with limited understanding, cerebral palsy or multiple sclerosis.
In some cases, the dental team prefer to use other ways of calming the patient. These can include simply talking, visiting the practice to meet staff, or even hypnosis. These can all be effective in making the patient less anxious.
How often should I visit the dental team for care?
It is important to visit the dental team regularly. This would normally be every six months, but some people need to visit less often and others more often. The dental team will be able to tell you.
The dentist may also recommend appointments with a dental hygienist, who will remove plaque and tartar from the teeth and offer advice on how to brush effectively and keep your mouth clean and healthy. The dental team may also offer advice to care givers about the dental care of others. It is very important to build a relationship between the dental team, the patient and their care giver. This can help greatly with people who have severe learning difficulties. Short but regular appointments seem to work better at building trust between the patient and the dental team than long appointments at irregular intervals.
It is recommended that children up to three years old use a toothpaste that contains at least 1000ppm (parts per million) of fluoride. Over the age of three, a toothpaste that contains 1350 to 1500ppm of fluoride is recommended. If the dentist thinks that the patient is particularly at risk of tooth decay, they may prescribe a toothpaste or fluoride varnish which contains a higher level of fluoride to offer more protection.
Special treatment may be available under the National Health Service (NHS) or might have to be paid for privately, depending on the practice. It is important to ask for an after treatment plan check-up and an estimate of charges at the time of the check-up. This will avoid confusion over payment later. NHS treatment is free for people getting benefits. If the patient is on a very low income, but does not claim any benefits, they may get help with charges by filling in an HC1 form. You can get this from either the dental practice or a doctor's surgery. You may also be able to get help towards eye tests among other things.


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{Cacio e Pepe Di Leonardo Vignoli} Leonardo Vignoli's Cacio e Pepe Pasta
Inspired by Trattoria Da Cesare Al Casaletto, Rome, Italy
Reprinted from Tasting Rome: Fresh Flavors and Forgotten Recipes from an Ancient City. Copyright © 2016 by Katie Parla and Kristina Gill. Photographs copyright © 2016 by Kristina Gill. Published by Clarkson Potter/Publishers, an imprint of Penguin Random House LLC.
Katie Parla
Katie Parla knows a thing or two about Rome—having lived and worked there for over 13 years—and she's shared her knowledge in the just-release bo...
Dinner, Lunch, Main, Noodles
Winter, Spring, Summer, Fall
Katie Parla knows a thing or two about Rome—having lived and worked there for over 13 years—and she's shared her knowledge in the just-release book, Tasting Rome. We asked her about her must-east places in Rome and she mentioned Trattoira Da Cesare Al Casaletto (where we ate together during Off Menu and that she talks about in her book) so, naturally, we asked her to share the recipe for one of those pastas here.
Cacio is the local Roman dialect word for Pecorino Romano, a sheep's-milk cheese made in the region since ancient times. Like carbonara, cacio e pepe is a relative newcomer to the Roman repertoire, first appearing in the mid-twentieth century. Pasta is tossed with an emulsified sauce of Pecorino Romano and black pepper that is bound by starchy pasta cooking water. Depending on the cook, the results range from dry to juicy. We love Leonardo Vignoli's saucy version at Cesae al Casaletto. He uses ice in a hot pan to obtain a creamy sauce, but we have adapted his recipe to obtain more consistent results in a home kitchen. Finely grated Pecorino Romano and very hot water are essential to a smooth sauce, while fresh, coarsely ground black pepper gives flavor and texture. The most important component of a flawless cacio e pepe, however, is speed. If the water cools before melting the cheese, the sauce will clump.
or tonnarelli
2 cups finely grated Pecorino Romano cheese
2 teaspoons Freshly ground black pepper
plus more to taste
Bring a large pot of water to a rolling boil over high heat. Salt the water. When the salt has dissolved, add the pasta and cook until al dente.
Meanwhile, in a large bowl, combine 1 1/2 cups of the Pecorino Romano, the pepper, and a small ladle of pasta cooking water. Using the back of a large wooden spoon, mix vigorously and quickly to form a paste.
When the pasta is cooked, use a large strainer to remove it from the cooking water and quickly add it to the sauce in the bowl, keeping the cooking water boiling on the stove. Toss vigorously, adjusting with additional hot water a tablespoon or two at a time as necessary to melt the cheese and to obtain a juicy sauce that completely coats the pasta.
Plate and sprinkle each portion with some of the remaining Pecorino Romano and pepper to taste. Serve immediately.
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Katie is a Rome-based food and beverage educator and journalist. Originally from New Jersey, she has an art history degree from Yale, a master's degree in Italian Gastronomic Culture from the Università degli Studi di Roma "Tor Vergata", a sommelier certificate from the Federazione Italiana Sommelier Albergatori Ristoratori, and an archeological speleology certification from the city of Rome. Katie's mission is to highlight great food and beverages, praise the people dedicated to feeding us well, and to get readers talking about what they are eating and drinking. She focuses special attention on Rome, where she lives, threats to local food culture, and critical reviews of restaurants and trends.
More posts by Katie
http://saltandwind.com/recipes/451-cacio-e-pepe-di-leonardo-vignoli-recipe


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Richard Henry Simpson Stilgoe, OBE (ur. 28 marca 1943) – brytyjski autor tekstów piosenek oraz komik. Jego najbardziej znanym dziełem jest współautorstwo libretta musicalu Upiór w operze.

Życie osobiste
Richard Stilgoe urodził się 28 marca 1943 w Camberley, Surrey Młodość spędził w Liverpoolu, gdzie występował m.in. w młodzieżowej grupie Tony Snow and the Blizzards występując w Cavern Club. Ukończył szkołę średnią Monkton Combe School w Somerset oraz studia w Clare College na Uniwersytecie Cambridge.

Jego syn,  Joe Stilgoe jest pianistą jazzowym oraz wokalistą.

Kariera

TV i radio
W roku 1966 grał  na West Endzie w musicalu Jorrocks jedną z ról epizodycznych. Stał się relatywnie znany dzięki programom popołudniowym w telewizji BBC Nationwide oraz That's Life! w których występował we własnych satyrycznych skeczach i piosenkach. W latach osiemdziesiątych występował w satyrycznym programie  A Kick Up The Eighties. Był znany z wyjątkowej łatwości w pisaniu tekstów w stylu kabaretowym (np. śpiewana intstrukcja szwedzkiego sekstelefonu, śpiewany poczet królów angielskich - pastisz występów King's Singers, improwizacje oparte o słowa podrzucane przez publiczność). Prowadził wiele programów radiowych w  BBC - Hamburger Weekend, Used Notes, Stilgoe's Around,Maestro and Richard Stilgoe's Traffic Jam Show.

Stilgoe jako wielki fan anagramów brał ponad dwieście razy udział w teleturnieju im poświęconym, Countdown. Prowadziłl również quizy w radio: The Year in Question, Finders Keepers (1981–1985) i Scoop (1981–1982).

Musicale
Niezależnie od działalności komika, Stilgoe zaczął pisać również teksty dla innych wykonawców i to właśnie ta działalność przyniosła mu największe uznanie.  W 1981 roku poznał Andrew Lloyd Webbera. Współpracował przy tworzeniu libretta do przebojowego musicalu Cats, w 1985 napisał libretto doStarlight Express, a dwa  lata później współpracował z Charlesem Hartem przy libretcie wielkiego hitu West Endu, kolejnego musicalu musicalu Webbera The Phantom of the Opera.  Później napisał jeszcze 2 musicale dla szkół: Bodywork oraz Brilliant the Dinosaur.

Działalność charytatywna
Przez ponad 20 lat prowadził  koncerty promujące młodzież  Schools Proms w Royal Albert Hall oraz brał udział w charytatywnych koncertach Royal Variety Performance. W roku 1998 stworzył fundusz Orpheus Trust, mający wspierać młodych artystów z różnymi upośledzeniami,  zapoczątkował także cykl koncertów, Stilgoe Family Concerts w Royal Festival Hall wspomagając występy młodych artystów. Jest również patronem towarzystwa  Surrey Care Trust w Milford - wspomagającym motywację do zmian w życiu i wspierającym poprzez fundusz kryzysowy mieszkańców w potrzebie.

Stilgoe przeznaczył również całość swoich tantiemów za Starlight Express na rzecz indyjskiej wioski, ze względu na popularność musicalu są to kwoty rzędu kilkuset funtów dziennie.

Inne
W latach 1998-1999 dzierżył ceremonialne lokalne stanowisko High Sheriff w Surrey, obecnie jest tam Deputy Lieutenantem. Jest fanem krykieta, od 2005 roku prezesemSurrey County Cricket Club, uprzednio prezesem klubu Lord's Taverners.

Nagrody
Stilgoe miał w swojej karierze dwie nominacje do nagrody Tony, zdobył trzy nagrody na festiwalu telewizyjnym w Monte Carlo oraz Prix Italia, otrzymał order imperium brytyjskiego. W 2012 otrzymał tytuł szlachecki Knight Bachelor za działalność charytatywną w Alchemy Foundation.

Przypisy

Linki zewnętrzne
 Orpheus Trust

Absolwenci Clare College (Cambridge)
Brytyjscy tekściarze
Urodzeni w 1943

redpajama_set_name: RedPajamaWikipedia


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Home Business REAP gearing up to file reasoned statement in EU against India
REAP gearing up to file reasoned statement in EU against India
Lahore (Muhammad Yasir) Preparations to file 'reasoned statement' in EU to contest the Indian efforts of securing exclusive rights of Basmati Rice is in full gear by the Rice Exporters Association of Pakistan (REAP), which thinks that Pakistan has a strong case as EU recognizes Pakistan as an authentic Basmati rice-growing region. Nevertheless, Current status of the case is that REAP has filed a Notice of Opposition on 07-12-2020 against India's claim on Geographical Indication (G.I) of Basmati in European Union.
The European Union has also acknowledged the filings of the 'Notice of Opposition' by REAP, said a spokesman of the Association here on Thursday. Rice Exporters Association of Pakistan (REAP) is fighting the battle against India's claim on geographical indicator (GI) of Basmati in EU. After the announcement of application of India for GI in European Journal, the first step was to stop India from proceeding further in its registration of GI in European Commission by filing a notice of opposition.
This is the first step which effectively maintains the status quo, whereby making the applicant's (India) approval of GI conditional on the decision of DG Agriculture European Commission (DG Agri).
The second step is filing of a 'reasoned statement' within 60 days of filing of the opposition notice. At the third step, hearings and other proceedings will start after this period of 60 days elapses, which will be in February 2021. The final decision on the registration of GI of Basmati will be delivered after the hearings. As the case in EU progress, REAP will keep on updating on all the developments. Basmati, being a centuries-old heritage of Pakistan, could not be allowed to be monopolized by India in the European market. Such a gross misrepresentation by India on the origins of Basmati is an attack on the values of fair competition among farmers and exporters in EU. Pakistan has a legal right to export Basmati with its original name in accordance with the practice in EU which is decades old, the spokesman concluded.
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Cheap flights from Dallas/Fort Worth (DFW) to Newcastle upon Tyne (NCL)
DFW — NCL
Cheap flights from Dallas/Fort Worth to Newcastle upon Tyne
How to find the cheapest flight from Dallas/Fort Worth (DFW) to Newcastle upon Tyne (NCL)
All the information you need to plan the best DFW to NCL flight in 2023: compare Dallas/Fort Worth to Newcastle upon Tyne flight prices with the expected peak season, weather and rainfall in Newcastle upon Tyne.
DFW - NCL
39.2 - 62.6 °F
July High demand for flights, 41% potential price rise
April Best time to find cheap flights, 35% potential price drop
$873 Average for round-trip flights in January 2023
$701 From Dallas/Fort Worth to Newcastle upon Tyne
What is the cheapest month to fly from Dallas/Fort Worth Airport to Newcastle upon Tyne?
April can be a great time to fly to Newcastle upon Tyne from Dallas/Fort Worth Airport with a flight ticket averaging about $1,081. Cheapflights users have found deals in the month of April for as cheap as $701. The most expensive prices can be found in the month of July.
When is the cheapest time to fly from Dallas/Fort Worth Airport to Newcastle upon Tyne (DFW - NCL)?
When flying from Dallas/Fort Worth Airport to Newcastle upon Tyne, the time of day you decide to fly out makes little to no difference towards the overall price you'll pay on tickets.
Cheap flights from Dallas/Fort Worth to Newcastle upon Tyne in January, February 2023
The best prices found for DFW to NCL flights for January, February
Dallas/Fort Worth to Newcastle upon Tyne 2023 flight deals
Cheap flights to Newcastle upon Tyne from Dallas/Fort Worth found for this year
Cheap Delta flights from Dallas/Fort Worth to Newcastle upon Tyne
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The cheapest one-way flights from Dallas/Fort Worth to Newcastle upon Tyne
Reviews of Airlines Servicing Newcastle upon Tyne
Great flight. Left gate early and arrived in Baton Rouge 6 minutes early.
Useful info, stats and facts about Dallas/Fort Worth to Newcastle upon Tyne flights.
What airport do you fly into for flights to Newcastle upon Tyne from Dallas/Fort Worth?
If you book a flight to Newcastle upon Tyne from Dallas/Fort Worth, you will be flying to Newcastle, which is the city's only airport. Newcastle upon Tyne (NCL) is located 5.4 mi from the center of Newcastle upon Tyne. There are 0 flights from Dallas/Fort Worth to Newcastle per day from 3 different airlines.
Which airlines flying from Dallas/Fort Worth to Newcastle upon Tyne have flexible cancellations due to COVID-19?
Due to the rapidly changing circumstances due to COVID-19, airlines like Delta, British Airways, and American Airlines flying from Dallas/Fort Worth to Newcastle upon Tyne have implemented new flexible cancellation policies.
Can I find flights from Dallas/Fort Worth Airport to Newcastle upon Tyne for under $700 on Cheapflights?
Yes, there are multiple flights from Dallas/Fort Worth Airport to Newcastle upon Tyne for under $700. The cheapest flight booked recently is on Delta for just $657, but on average you can expect to pay $1,235.
What are the cheapest airlines flying from Dallas/Fort Worth Airport to Newcastle upon Tyne round-trip?
Our cheapest airline available from Dallas/Fort Worth Airport to Newcastle upon Tyne is Delta. Our recent data shows that you can find tickets from as low as $657 on this airline. Additional options worth looking into are British Airways and American Airlines, starting at $1,088 and $1,421 round-trip. Delta is 39% cheaper than the average price of flights available within the next 90 days to this destination.
What are the most popular airlines that fly from Dallas/Fort Worth Airport to Newcastle upon Tyne?
The most popular airlines flying from Dallas/Fort Worth Airport to Newcastle upon Tyne are American Airlines, British Airways and Delta. Something to consider prior to booking your flight.
What are the cheapest flights from Dallas/Fort Worth Airport to Newcastle upon Tyne?
On average you can expect to pay $1,356 for a flight from Dallas/Fort Worth Airport to Newcastle upon Tyne. The cheapest flight overall is $1,356 while the most popular route, (Dallas/Fort Worth - Newcastle upon Tyne) is currently priced at $615.
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Information is based on travel restrictions from Dallas/Fort Worth to Newcastle upon Tyne
Most visitors from Dallas/Fort Worth can enter Newcastle upon Tyne without restrictions.
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Can I fly back to Dallas/Fort Worth from Newcastle upon Tyne?
Returning to Dallas/Fort Worth from Newcastle upon Tyne
Visitors from Newcastle upon Tyne are not required to present a negative COVID-19 PCR test or antigen result upon entering Dallas/Fort Worth.
Visitors from Newcastle upon Tyne are not required to quarantine after entering Dallas/Fort Worth.
Alternative routes for Dallas/Fort Worth to Newcastle upon Tyne
Dallas/Fort Worth to Manchesterfrom $137 pp
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Top 2 airlines flying from Dallas/Fort Worth to Newcastle upon Tyne
The chart below displays the airlines Cheapflights users traveling from Dallas/Fort Worth to Newcastle upon Tyne choose most often. Cheapest and average prices are based on aggregated data from the past 12 months.
Delta Cheapest$708 Average$888 FlexibilityNo cancel fee On-timeN/A COVID-19 safety
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British Airways Cheapest$839 Average$1,267 FlexibilityNo cancel fee On-time70% COVID-19 safety
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Left-handedness is much rarer than right-handedness.
The people who fall into the first category have dominant left hand and use it when executing tasks.
Scientists cannot find an exact reason why some individuals are left-handed, but it's proved that genes play role in the left-handedness of only 25% of the people. According to a British study, the fetuses of stressed pregnant women would touch their faces with their left hands most of the time. Another Swedish research from 2008 supported this theory, reporting that women who were depressed or stressed during their pregnancies would probably have left-handed children.
Another interesting fact is that left-handed people are actually a very small part of the population, only about 10 %. However, they compensate for being a minority with outstanding right-handers in many ways.
Left-handed people tend to be more creative. They are capable of thinking out of the box more often and give unconventional solutions to different problems. It's little surprise then, that they are better at arts and usually pursue creative careers.
Lefties prove to be better at sports. One reason for this may be because they usually train against right-handed opponents while right-handers don't have as many opportunities to practise against left-handed players.
Left-handed people are more sensitive. They suffer from Posttraumatic stress disorder more often than the rest. The cause of this might be because they are more susceptible to fear and experience emotions related to it much more deeply.
The same goes when talking about mental disorders. It turns out left-handed people are much more prone to them than the righties. A not small percentage of schizophrenics are lefties and the same is valid for people who suffer from mental disorders that cause abnormal thinking and perceptions.
Left-handers tend to be better fighters, especially in hand-to-hand matches. They are physically stronger and might pull their opponent over more easily. Having a left-hook is an undeniable advantage in boxing, for example.
In the past scientists thought left-handers had weaker immunities. While, on the whole, this idea had been proven wrong lefties suffer from Crohn's disease and ulcerative colitis, which are related to stomach, more often than right-handed people.
In a pair of twins, it's more probable to have a right-handed and a left-handed child. While this is not valid for many other characteristics when talking about right and left-handedness, the possibility of twins being mirror-images is as twice as strong.
Lefties like the major part of the right-handers are left-brained and also use this part to process language. Only because righties are left-brained doesn't mean a lefty would necessarily use their other side of the brain. This is a legend about left-handed people that has already been demystified.
A big part of the left-handed people have more or less introvert personality. They are relatively closed in themselves and can behave unconfidently in moments when they need to be social. They are more sensitive and are usually worried about not doing things as they should.
The idea that left-handedness increase the chances of becoming an alcoholic was very popular until not long ago. This has been proven wrong but it's true that lefties get drunk more easily than the right-handers.
Finally, we should remember that August 13 is proclaimed as International Left-Handers Day, by Lefthanders International. It was first celebrated on the 13th of August 1976. This is a day dedicated to honouring their way of life, it is supposed to spread awareness of the difficulties faced by left-handers in a right-handed world and celebrate their uniqueness.


You need to sequence all steps and stages into a logical and clear list. This paper just explains how something is done. You can even write a process analysis essay about how to write a process analysis paragraph. There are also lots of other interesting, and even funny process analysis essay topics students love to use.


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Universities Wales Weekly Wrap Up 9 January 2015
Welcome to the Universities Wales Weekly Wrap Up, keeping you up to date with all the latest developments across higher education in Wales.
Ideas for Life: Swansea University
Read more about how research at Welsh universities is changing our world for the better.
Ideas for Life: The Open University in Wales
Welsh universities highlight value of university research at home and abroad
Universities Week 2014 launches today, 9 June, highlighting some of the ground-breaking research currently taking place at universities across Wales.
Using our international research collaboration to market Wales
Higher education is an increasingly competitive business, but Welsh universities are making their presence felt, as Professor Peter Halligan, head of strategic futures at Higher Education Wales, explains.
HEW Weekly Wrap Up 9 May 2014
Welcome to HEW's Weekly Wrap Up, keeping you up to date with all the latest developments in the higher education sector in Wales.


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How John Bonham helped Robert Plant when his son died
Led Zeppelin vocalist Robert Plant lost his 5 year-old son Karac, victim of stomach virus back in 1977 while the band was on tour in the United States. In an interview with Classic Rock magazine, the singer recalled how the late drummer John Bonham helped him and his family to get back on their feet.
How John Bonham helped Robert Plant when his son died, according to the singer:
"In 1977 we lost our son, Karac [he died of a stomach virus while Led Zeppelin were on tour in America]. He was only five years old. I'd spent so much time trying to be a decent dad, but at the same time I was really attracted to what I was doing in Zeppelin."
"So when he bowed out, I just thought: "What's it all worth? What's that all about? Would it have been any different if I was there, if I'd been around?" So I was thinking about the merit of my life at that time, and whether or not I needed to put a lot more into the reality of the people that I loved and cared for – my daughter and my family generally. So yeah, I was ready to jack it in, until Bonzo came along."
He convinced you otherwise?
"Yeah. He had a six-door Mercedes limousine and it came with a chauffeur driver's hat. We lived five or six miles apart, not far from here, and sometimes we'd go out for a drink. He'd put the chauffeur driver's hat on and I'd sit in the back of this stretch Mercedes and we'd go out on the lash. Then he'd put his hat back on and drive me home."
"Of course, he'd be three sheets to the wind, and we'd go past cops and they'd go: "There's another poor fucker working for the rich!" But he was very supportive at that time, with his wife and the kids. So I did go back [to Zeppelin] for one more flurry."
Plant enjoyed great success with Led Zeppelin from the late 1960s to the late 1970s and developed an attractive image as the charismatic vocalist of rock and roll, similar to contemporaries like Roger Daltrey of The Who, Freddie Mercury of Queen , Mick Jagger of the Rolling Stones and Jim Morrison of The Doors.
With his long blond hair (which later gave him the nickname among Golden God fans) and a powerful, bare-chested appearance during performances. Although Led Zeppelin disbanded in 1980, Plant occasionally collaborated with Jimmy Page on several projects during that period, including forming a group of short-lived stars with Page and Jeff Beck in 1984, called Honeydrippers.
Related Topics:articles, CLASSIC ROCK, Featured, hard rock, interviews, john bonham, led zeppelin, robert plant
Gene Simmons shows his $25 million mansion that is for sale
Ronnie James Dio widow recalls Ritchie Blackmore introduced her to Dio


Water was restored in Rudyard Tuesday afternoon after a water main break Monday left the town dry.
The break resulted in school closures of the North Star middle and high school in Rudyard. Students are back at school today, Hill County Commissioner and Rudyard resident Mike Wendland said this morning.
The concern with the repair, which was being worked on near the railroad track by Wells Fargo and Farmer's Union store, was if the leak was too deep. The frozen ground had made it difficult for C & C Excavation Inc. to make headway when it began work.


Crazy for Jason Derulo hits such as "Talk Dirty," "Wiggle," and "Want to Want Me?" You're in luck, because he returns to Manila this May with LMFAO's Redfoo in tow.
Aussie pop-rock sensations Luke, Calum, Ashton, and Michael head to Manila for their Sounds Live Feels Live tour, with Scottish indie rockers Twin Pines set to open for them. If you don't think you're too old to fangirl over these teen idols, by all means, get those tix!
K-pop junkies, rejoice! BEAST is holding its first Manila "fan meeting" this month, and it won't just offer live performances—it'll allow fans to get up close with the Korean pretty boys as well.
Get painted beyond recognition at this huge paint party featuring globetrotting acts Galantis, Dannic, Firebeatz, and Kura. Local pros Marc Marasigan, Ron Poe, Deuce, Kat DJ, Funk Avy, Katsy Lee, Callum David, Those Damn Nerds, Toons, Everyday People, Pong Santos, and Franco Zarate complete the lineup.
Also a color festival, Chroma promises insane sets by foreign headliners Timmy Trumpet, Stadiumx, and Tujamo, as well as Manila's own beatmasters Ace Ramos, Mars Miranda, David Ardiente, Therese Garcia, Tom Taus, Funk Avy, Cathy Frey, Lucio Pua, and Sai Dy.
Closeup's annual summer rave says adios with its final edition this year, and closing off the event's phenomenal three-year run are Belgian EDM greats Dimitri Vegas and Like Mike.
The annual outdoor gathering of indie music lovers returns for its fourth year, this time with a fantastic lineup of foreign and local acts including Death Cab for Cutie, Bon Iver, The Naked and Famous, San Cisco, Panama, Blackbird Blackbird, CRWN, Jess Connelly, Cheats, Chad Valley, Commandeur, Jensen and the Flips, and Curtismith.
The Florida-based alt rockers known among indie-loving communities for their emotive tunes will be giving fans the feels as they head this way this April.
The Icelandic five-piece behind the indie anthem "Little Talks" brings their brand of exultant indie folk to Manila for the first time this May.
The first Paradise International Music Festival boasts a lineup spanning hip-hop to EDM to pop to OPM: Kanye West headlines; Rudmental, Austin Mahone, and Afrojack are among the foreign acts; and Up Dharma Down, Hale, Itchyworms, Maude, Giniling Festival, Yolanda Moon, Clara Benin, and DJ Jessica Milner make up the local acts.
This yearly musical pilgrimage to the foot of Mount Malasimbo once again calls on fans of soul, jazz, and world music to party outdoors to sick sets by homegrown talents as well as acts from the UK, Korea, and Australia.
For Holy Week, the Malasimbo group puts forth another shindig boasting funk, hip-hop, house, and electronic acts from all over the world; dance performances by talented crews, and a lights show that promises to be uh-mazing.
This three-day fest lets you groove beachside to music by 30 all-local bands and DJs including Marc Marasigan, Travis Monsod, Ebe Dancel, Hale, Ace Ramos, Motherbass, Moonstar 88, The Zombettes, Katsy Lee, Cheats, Quest, and many more.


Here's what I know to be true after yesterday's Women's March in DC: We are the country I thought we were.
We are diverse, we care about each other and our future, and we are kind.
We are afraid, but we will rise to the challenge to fight for those issues we deeply care about—women's issues, inclusivity over isolationism, the future of the country and planet we're leaving for our children, and so much more.
We are diverse. We are women and men; we are straight, gay, lesbian, trans, and any expression of gender we want to be; we are Jewish, Muslim, Christian, and any expression of religion or non-religion we want to be.
There was a dark-haired, dark-skinned woman wearing jeans and a white jacket. She had with her four pre-teen kids and she held a sign that said THIS IS WHAT A MUSLIM FEMINIST LOOKS LIKE. It was obvious how proud her kids were, how happy they were to be there as people walked by and nodded, gave her high-fives, clapped her on the back. America is for this woman and her children.
There was a group of four trans men marching with no shirts, proudly showing the scars of their breast-removal surgery. They fist-pumped as some people gaped and others cheered. America is for those men.
In the middle of a throng of people so massive and crushing, before police finally opened the barricades to the National Mall, a woman went in to labor. The crowd was thick, chest-to-chest, barely room to squeeze a foot forward in either direction. But as she came through, the people parted. Voices rose and people cheered and others comforted her. America is for this baby, who received one of the most beautiful initiations into this life I can imagination.
There was an African American family—grandparents, their two grown daughters, and two grandchildren, maybe seven and nine. We stood along 17th Street NW together waiting to enter the march. I was passing out buttons that said WOMEN'S VOICES MATTER, WOMEN'S CHOICES MATTER. As I handed buttons to his granddaughter and daughter, the elderly man stretched out his gloved hand. I placed a button in it and said, "I hope you'll wear it with pride," and his wife looked at me and said, "He will. He's been here before." When I met the old man's eyes, I felt as if he were bringing the spirit and legacy of whatever previous protests he'd witnessed—perhaps The Great March or The Million Man March—to this march for his daughters and granddaughters. America is for this family.
I went with a group of authors from She Writes Press, and with my wife. I wanted to be there for women everywhere, because I will fight for women's voices to be heard, and for the rights we stand to lose. I will be one of the many vocal dissidents of this administration and its destructive policies against a people, a country, and a planet I love. But I needed to be there for my family—for my wife, for our rights, for our sons. For our safety and legitimacy, and for our six-year-old's education and all three boys' futures. To be one of the hundreds of thousands of bodies that contributed to the crush of people coming to Washington to say, simply: What's happening here is not okay with me. Those of us who showed up in cities big and small around the world yesterday—640 demonstrations in the States alone—were together as one.
Globally, we will be vigilant.
Here in America, we will not stand idly by as our rights and freedoms are stripped away from us. Here in American, will not be fooled by a slogan that professes to Make America Great Again when it's really about Making White Men First Again.
I am grateful beyond words to have witnessed what I did yesterday. It gave me the renewed faith I needed. We are not screwed, as I've pessimistically been prone to thinking in the past few months. We just have a lot of work ahead of us. And yesterday proved to me just how well equipped We, the People, are to get done what needs to be done.


Today, June 13th, marks the day of the official launch of TerryStorch.com. One year ago Scoble came by FC [link], and that really started the Blogging bonanza for me. This has been a great year of blogging and I have learned a lot. Here are some of the stats from year one.
Perry Noble, What's Working For You?
This has been a fun year, and I really look forward to seeing where we go from here. I really appreciate you guys reading and commenting on a regular basis. Know that I am always looking for new ideas, so if you would like to see something here please let me know about it!


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Show Events: Everywhere Within 25 Miles New York, NY, US Los Angeles, CA, US London, GB Auckland, NZ Washington, DC, US Palm Springs, CA, US New Haven, CT, US Prague, CZ Copenhagen, DK Dallas, TX, US Toronto, ON, CA Pisa, IT
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Never Alone: Video Games and Other Interactive Design
Sep 10 - Jul 16, 2023
Scandinavian Design and the United States, 1890–1980
Oct 9 - Feb 5, 2023
Yinka Ilori
Sep 15 - Jun 25, 2023
London, GB
The Architecture Drawing Prize 2023
Feb 8 - May 7, 2023
The Restorative City: Designing New York City with Health at the Center
Fairy Tale Architecture
One Life: Maya Lin
Sep 30 - Apr 16, 2023
Justice is Beauty: The Work of Mass Design Group
Apr 9 - Mar 7, 2023
New Practices New York
Sep 30 - Feb 4, 2023
Denise Scott Brown: A Symposium
Feb 8 - Feb 8, 2023
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Colloqate, WIP, FUNdaMENTAL, and Assemble showcase new exhibition at Art Omi celebrating the 'power of advocacy'
OMA completes 'multi-dimensional' exhibition design for Dior in Japan
'Give Me a Sign: The Language of Symbols' at the Cooper Hewitt explores the legacy of design pioneer Henry Dreyfuss
In Helsinki, an award-winning pavilion prepares to serve its second biennial
Lebbeus Woods: ECOLOGIES, 1984 - 1990
Fri, Jan 06, 2023 - Sat, Feb 04, 2023
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Resilient and Responsible Architecture and Urbanism (RRAU) – 5th Edition
Wed, Apr 26, 2023 - Fri, Apr 28, 2023
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ASSEMBLE:ELBMESSA
Architecture & Design Film Festival Online 22/23
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exhibition usa new york london europe uk conference california # architecture los angeles new zealand symposium
Tuesday, Jan 31, 20237 PM - 8:30 PMEDT
The Great Hall, The Cooper Union, 7 East 7th Street New York, NY, US New York, NY, US | The Great Hall, The Cooper Union, 7 East 7th Street
Members of the London-based collective visit New York to present their recent projects.
Current Work is a lecture series featuring leading figures in the worlds of architecture, urbanism, design, and art.
Assemble is a collective working across disciplines including architecture, research, design, and public art. Founded in London in 2010 to undertake a single self-built project, Assemble has since delivered a diverse and award-winning body of work. The collective maintains a democratic and cooperative practice that enables built, social, and research-based projects at a variety of scales.
In the words of a 2015 Metropolis profile, Assemble is "working to create an open, flexible, participatory, and exciting city, based on architectural projects that are ad hoc, handmade, built by volunteers, and fundamentally of their respective, very urban sites."
This event will not be live streamed.
Free for League members; $15 general admission.
FF – Distance Edition: LMN Architects
USC Architecture Engaged: Paul Farber Lecture
Wed, Feb 8 - Sun, May 7, 2023
Wed, Feb 8 - Fri, Feb 17, 2023
Dimitra doesn't want to move: aging in-place, localized communities and the adaptive re-use of traditional building typologies in villages in Northern Greece Symposium
Modernism Week 2023
Thu, Feb 16 - Sun, Feb 26, 2023
People, Processes, and Practice: Technology + the Business of Design
Andrea Branzi's "Contemporary DNA"
Thu, Mar 2 - Sat, Apr 22, 2023
FF – Distance Edition: Leers Weinzapfel Associates
Wed, Apr 19 - Fri, Apr 21, 2023
Isokon Symposium
Advancing Multi-family Design and Construction 2023
Mon, Apr 24 - Wed, Apr 26, 2023
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The post Los Angeles FC 23/20 to beat Minnesota United in Mondays MLS matchup appeared first on Soccer News.
The Los Angeles County Department of Public Health said Monday it's looking into at least five confirmed cases of the illness among its residents.
Sometimes the best motorcycle rides are right in your own backyard. That's the premise of the Motorcyclist magazine Alley Rally. Taking place once a year, the event is a guided turn-by-turn tour..
According to Reuters.com, "The Academy of Motion Picture Arts and Sciences has voted not to change its rules for winning an Oscar, Hollywood's top prize." This will continue to be a challenge for..
Some lucky fans and reporters saw 'Avengers: Endgame' Monday night at the film's Los Angeles premiere. According to Business Insider, first reactions say the film is "epic," "emotional," and..


You can play The Light Temple free online car games at fogames. It has tags: Puzzle,Platform,Water,. It has 1692 total plays and has been rated 86.1% (34 like and 10 dislike), find the fun and happy day. If you want to play more car games, we find some of the game: Fireboy and Watergirl in the Light Temple, Light It, The Crystal Temple and Classic Hashi Light. To play other games, go to the puzzle games page, the platform games page, or just check out the new games or best games page.
Play The Light Temple online games, Fun and Happy.


Marcia Schneider is moving to Portland!
Join us on Saturday the 29th as we send Marcia and Howard off on their next life adventure. Food, drinks, friends, and everything you would expect from a Valley Grassroots party!
Click the big green button below to RSVP.


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Judd Foundation's mission is to maintain and preserve Donald Judd's permanently installed living and working spaces, libraries, and archives in New York and Marfa, Texas. The Foundation aims to promote a wider understanding of and appreciation for Judd's artistic legacy by facilitating public access to these spaces and resources and by developing scholarly and educational programs.
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If you love to play Lego, probably you love to assemble furniture too… It is almost the same – just put each item in its place and voala! Now you have something beautiful and more importantly – that is created from your own hands. If you are creative and with the greatest pleasure are dealing with such activities, then you surely have assembled all the furniture in your home and office as well. And we are 100% sure that if the answer is "yes", even your neighbours are using you to help them with their furniture. Still, you are the skilled guy who can make from several pieces of wood, beautiful furniture to be proud of!
When it is time for furniture assembly near me, do not hesitate and call a specialized company to help you with this. It will be easier for you to use such services instead to assemble all the new furniture single-handed. We agree that the last one might be the most pleasant think you've ever done, but do you have so much time at all? To be everything perfect and made in a professional way, you must take more time, as well as to make sure that you do not miss something… If you make a mistake and some of the bolts is not in place, you risk to have a frame-built furniture that is about to fall apart!
That's why, you should take care of the good furniture assembling and not to neglect the professional skills and knowledge of the companies dealing with handyman services! Do not get us wrong – we do not say that you are not good enough with the assembly… Maybe, you are even the best one among your friends and relatives, but if you think to hurry, as well as to assemble your new wardrobe in the fastest way possible, please, just do not do this! If you have no time, leave this job to the handyman companies that are on your disposal when you need the most!
We are certain in one thing: if you want quality furniture assembly, do not hire the first company you see. Make a short Internet researching and only then decide who to trust! In many cases, people make a mistake when choosing a "reliable" company and as a result, they are forced to even change the new furniture. Because it is already irreversibly broken! Be careful and enjoy your cozy home ;).


Lakeside Farm is located in an area of outstanding natural beauty, lovingly converted into one of Britain's top naturist holiday parks, offering self catering accommodation including static caravans, cabins and studios for hire plus caravan and camping pitches with hook-ups. We are also open to day visitors.Lakeside is open all year and occupies a 13 acre site of naturally landscaped open sunny grounds and a 2.5 acre boating / fishing lake complete with pedalo and surrounded by 7,000 trees near Skegness, Lincolnshire, uk.
We have a very comfortable clubhouse, and a small shop for essentials and a regular program of events. We have good shower and toilet facilities. Once at Lakeside all facilities are free to use, sauna, hot tub, even the pool table is set on free play.
We have a nine hole putting green, a large hot tub and sauna plus a five acre nature reserve complete with picnic area. We offer free wifi and an easy online booking system on our website www.naturistholiday.co.uk and our website also as hundreds of photos of lakeside.
There is a visitor's' kitchen, with fridge and freezer and microwave. Access is across private driveway and visitors will be given access details before a visit. we welcome visitors from all over the world and we you like you to visit us too.
How to get there Please visit www.theaa.com and put in our post code PE23 5BQ for directions from your home. if you can travel via Spilsby the roads are less bumpy.
and find some where to park. The reception is located at the front of the bungalow, please press reception bell for our attention. Thank you.


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Extremist groups, drug cartels in South Asia add to India's many security concerns
Growing coordination among extremist groups and drug cartels in South Asia is another of India's many security concerns.
Farzand Ahmed
ISSUE DATE: January 7, 2002
SPREADING RED: Maoist attacks in Nepal coincided with those in India
The day Nepalese Prime Minister Sher Bahadur Deuba spoke to Indian Prime Minister Atal Bihari Vajpayee about declaring Emergency in Nepal was also the day Krishna Bahadur Mahara, chief negotiator for Nepal's Maoists, flew into Delhi.
He was here to meet his comrades - and there are enough of them in India and the neighbourhood to begin worrying even the unflappable George Fernandes, India's defence minister.
Mahara returned to Kathmandu a day before Emergency was promulgated in Nepal on November 26, and immediately disappeared. Meanwhile in India, the Maoist Communist Council (MCC) held an "open trial" of three men before a crowd of 5,000 in Ambikapur district in Chhattisgarh.
The men were tried for misusing the MCC's name for monetary gains. They were beaten into confessing their crime. A death sentence was passed and the three were promptly killed. In Andhra Pradesh, the People's War Group (PWG) chose more high-profile targets.
A granite unit belonging to Union Minister of State for Defence U.V. Krishnam Raju was attacked. The Heritage Milk chilling unit owned by Chief Minister N. Chandrababu Naidu's family was blown up.
Other targets included a Tata Tea unit and a manufacturing facility of Coca-Cola. Orissa had a state minister's house attacked. Was it a coincidence that all this should happen at a time when the Maoists in Nepal battled the Nepal Army, which is receiving support from India? Did Mahara's visit have something to do with the events?
The Indian Government banned the PWG and MCC under the controversial POTO. Soon after, the responsibility for monitoring the entire 735-km Indo-Nepal open border in Bihar was handed over to the Special Services Bureau (SSB) and the state Government was asked to cooperate with it in keeping a watch on the Maoists.
Nine SSB battalions comprising 8,000 jawans were moved to eight north Bihar districts adjoining the international border. The Defence Ministry has also decided to deploy the army to check any attempt by Maoist guerrillas from Nepal to sneak into India. According to Fernandes two more army stations will be set up in the Bihar.
The action has come, as always, belatedly. For some time now, intelligence reports had been talking of Maoist outfits in India, Bangladesh, Sri Lanka and Nepal working towards a coordinated campaign against the governments in all these countries.
They have formed an umbrella organisation called the Coordination Committee of the Maoist Parties and Organisations of South Asia (CCOMPOSA) to closely monitor and synergise the activities of nine guerrilla outfits in South Asia.
These organisations include the MCC, PWG, Revolutionary Communist Centre of India (ML) and the RCCI (Maoist) in India, the CPN (Maoists) in Nepal, the Maoists Punar-gathan Kendra and Bangladesh Samyabadi Party in Bangladesh and the Communist Party of Ceylon (Maoists) in Sri Lanka. These outfits have established strong ties with United Liberation Front of Asom too.
The Maoist groups are not isolated fronts but are part of a multilayered, international network. Like Nepal's Maoists, the PWG and MCC are affiliates of the Revolutionary Internationalist Movement (RIM), a London-based umbrella organisation of Maoists and narcoterrorists the world over.
According to the Executive Intelligence Report, RIM has strong ties with drug cartels, including the Afghansis in Afghanistan and the Sao Paulo Forum of South America. Organisations that formally belong to RIM include some of the most brutal narcoterrorist gangs in the world, like Peru's Shining Path.
Closely allied to RIM are the Kurdish Workers Party (PKK) and the Armenian Secret Army for the Liberation of Armenia (ASALA)- both ruthless killer gangs that finance their activities through opium trafficking, in partnership with the Afghansis and Pakistani groups.
Both the PKK and ASALA were considered important pawns in RIM's plans for geopolitical destabilisation in the southern tier of the former Soviet Union. ASALA ostensibly fought for a "Greater Armenia" to be carved out of sections of Turkey and Azerbaijan, while the PKK pushed for a separatist Kurdistan, culled from Iranian, Turkish and Iraqi territories.
Chilling facts about the operations and the international connections of RIM too have started coming out. The EIR pointed out that Sikh separatists who assassinated prime minister Indira Gandhi on October 31, 1984 were among the leading RIM allies in South Asia.
The Liberation Tigers of Tamil Eelam, which masterminded the assassination of Rajiv Gandhi on May 20, 1991, was another RIM ally and a crucial component in the region's burgeoning guns-for-drugs trade. These groups are also part of the Afghansi apparatus, and both groups attended the founding conference of RIM in London in March 1984.
6,000 people have died in Naxalite violence in Andhra Pradesh in two decades.
However, in its publications and declarations RIM claims to be "an international political formation which attempts to regroup the Maoists within the International Communist Movement".
For years, its headquarters and publishing operations were located in Russell House in Nottingham, England, named after Bertrand Russell. RIM's journal, A World to Win, was published for years by Russell Press, an affiliate of the one-worldist Bertrand Russell Peace Foundation.
According to intelligence reports, the coming together of the various outfits helped these underground outfits to extend the playing field of the Maoists from Hyderabad to Kathmandu and from Colombo to Dhaka.
In fact, the cross-border confederation enables Maoists in one country to get help from other south Asian countries. This would means that extremists could trouble India from across the Nepal and Bangladesh borders.
What is worrying for India is that Pakistan's ISI has already established strong bases in these countries. Though both the MCC and the PWG are at loggerheads in Bihar and Jharkhand, their battle is not on ideology, only a skirmish to establish a supremacy over the other in bid to encroach on that group's territory.
A senior Bihar Police officer admitted that the transborder confederation of extremists poses a serious threat to the security and the integrity of the nation. In a significant move the MCC- traditionally active in central and south central Bihar districts-has chosen Sheohar district in north Bihar as its new base.
Incidentally, the district borders Nepal, facilitating the movement of armed guerrillas across the border. "Earlier there was absolutely no presence of Naxalites in Sheohar, but now the MCC is active there. This is a cause for worry," admits Bihar Inspector-General of Police (Operations) Neelmani.
The MCC has already made its presence felt in another north Bihar district Darbhanga, which too is close to Nepal. Amid the many tensions in a volatile subcontinent, the menace of the extreme Red is often forgotten.
Yet these groups too have waged war against India now for at least two decades, claiming the lives of over 6,000 people in Andhra Pradesh and 12,000 in Bihar. And with synergy becoming a buzzword for Moaist extremists as much as corporate captains, it is unlikely this war will end soon.


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13-Year Old Wonderkid Youssoufa Moukoko's Record This Season Is Insane
Last updated 11:30 AM, Thursday March 22 2018 GMT
You'll probably want to make of a note of Borussia Dortmund's 13-year old wonderkid Youssoufa Moukoko because he could well be one to watch out for.
The young prodigy hit headlines in August 2017 when he made the step up to the Bundesliga Under 17, playing against kids much older than him.
Watch Moukoko's ridiculous four-goal haul against Viktoria Koln:
Credit: YouTube/SniperCompsHD
But he did so with distinction, marking his debut at Under 17 level by bagging a brace in the first game of the season against SG Unterrath as his side ran out 3-0 winners.
He well and truly set the bar in his first game at a higher level, but has replicated his heroics in games that followed in what has been an insane campaign so far.
Image: PA
In 15 games for the Dortmund Under 17's, he's netted an absurd 27 goals and also chipped in with four assists. To make you feel very old, the kid was born in 2004 and he's tearing it up!
#Dortmund have got a player is 13years old & he is playing in U-17..
He is a BEAST, scored 27goals in 15games !!!!!!!
Youssoufa Moukoko !! pic.twitter.com/rqlKvjKJlQ
- mÖi ArsenaL (@mOiArsenal) January 26, 2018
13 year old Dortmund striker Youssoufa Moukoko has played 15 games for their U17 team, his record so far...
Goals: 27
Assists: 4
One to watch? pic.twitter.com/CdIo1CO9Oc
- Footy Accumulators (@FootyAccums) January 26, 2018
Signed from St Pauli in 2016, Moukoko netted 33 goals in 21 matches in a single season for the Under 15's BVB side and his progression sees him having a bagful of goals for the Under 17's despite being four years junior.
Dortmund have a great track record with young players in recent times and it's no surprise that the club think he has the ability to join an esteemed list that includes the likes of Mario Gotze, Christian Pulisic, Nuri Sahin and Felix Passlack.
The age of the Cameroon-born striker has come into question, with some deeming him to be older than his birth certificate - something his father, Joseph, refutes.
"Right after his birth, I registered him with the German consulate in Yaounde [Cameroon's capital]," he said to the official Dortmund website, via ESPN.
"The boy can't be older because his mum, Marie, is only 28."
Topics: Football News, Football, BVB, Youssoufa Moukoko, Borussia Dortmund, Germany, Bundesliga
Aubameyang Has Confused The Sh*t Out Of Arsenal Fans With Cryptic Post
What Lionel Messi Did In Build-Up To Goals Vs Espanyol Proves He Does Everything
Paris Saint-Germain's Lucas Moura Has Agreed Personal Terms With Spurs
Bayern Star Reveals He Is Interested In Joining PSG In The Future


text:
Q: Cloud data fusion Permission denied due to datastream.connectionProfiles.discover I am trying to create a cloud data fusion replication job from oracle to bigquery. Receiving the below error.

Failed to connect to the database due to below error :
io.grpc.StatusRuntimeException: PERMISSION_DENIED: Permission
'datastream.connectionProfiles.discover' denied on
'projects/<>/locations/us-central1/connectionProfiles'

Following the steps mentioned in the official google documentation.
I was able to grant the Datastream Admin role to the dataproc service account.
The cloud data fusion service account is not available in this project's IAM page. Not sure how to assign "Datastream Admin role" to the data fusion service account.

Any help is appreciated..

A:
Found it we assign roles by the above way.

redpajama_set_name: RedPajamaStackExchange


text:
Hemisaga is a genus of insect in family Tettigoniidae. It contains the following species:
 Hemisaga elongata
 Hemisaga lucifer
 Hemisaga vepreculae

References

Tettigoniidae genera
Taxonomy articles created by Polbot


As Octavia fights for her life Arkadia is forced to prepare for an Ice Nation assault, and Clarke rides out to entreat with Roan for peace. Abby's attempts to create Night Blood inside Becca's laboratory is complicated when Raven has a seizure, leading to a startling discovery.
Clarke (Eliza Taylor) makes a desperate plea with a former allied force in an attempt to avoid a war and ensure the survival of her people.
Bob Morley, Paige Turco, Marie Avgeropoulos, Devon Bostick, Lindsey Morgan, Christopher Larkin, Richard Harmon, Zach McGowan, Isaiah Washington and Henry Ian Cusick also star. John F. Showalter directed the episode written by Morgan Gendel (#405).


Have you ever imagined being part of a spaceship launch sequence? With the Australian Space Agency (ASA), Mars Recovery Vehicle (MRV) Presentation you will have the chance to realize your dreams.
How are we connected to the universe? What is above our heads? How do we know and how can we see it?


Special event participation discounts such as our Limerick Community Day Business to Consumer event and our Annual Business to Business Expo event.
Great sub groups such as our Young Professionals Network and Women in Networking.
An avenue to publicize any events and specials that your business may be having through our facebook page, our weekly email blast and our quarterly newsletter.
Community involvement; A sense of pride in giving back to our community with events such as our Halloween Parade, Limerick Community Days and our Fashion show that raise money for local charities.


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Summary: Mounties lost their first match of 2014 last weekend going down 32-16 to the Panthers. Despite the loss, Mounties held on to their spot on top of the table, but it now leaves us with no unbeaten sides after five rounds of play. The Knights will be fresh for their trip to the capital after having had the bye last round, and they also head south with winning form after they have won back to back matches following a round two loss, ironically enough, to Mounties. With both of these sides currently sitting on eight competition points and only separated by for and against, there is a very real possibility that the winner of this clash will find themselves on top of the ladder at rounds end. The Knights welcome back Nathan Ross from injury for this one and will also be further aided by the addition of off season recruit, Matt Minto. Mounties have named Sauiluma, O'Donnell and McIllwrick who were all late withdrawals last week up in Penrith, and this should see them back to almost full strength for this top four clash. In this corresponding match at GIO Stadium last year, it was a 28-16 win for the home side.
Once was a Raider: One player for Newcastle who will be out to impress is former Raider, Travis Waddell. The Indigenous All Stars representative was unwanted by the club at the end of 2012 and eventually made his way to the Knights. Most players fire up for clashes against their former clubs, but particularly when there was a less than amicable parting of ways. Look for Waddell to be one of the Knights best come Saturday.
Summary: After a sluggish start to their 2014 campaign, the Penrith Panthers appear to have righted the ship in recent weeks and are beginning to show the kind of form that prompted many to consider them the team to beat in the VB NSW Cup before a ball was even kicked. They will have their hands full on Saturday though when the third placed Tigers head to the foot of the mountains. The Tigers have perhaps been the surprise packet of the opening five rounds as they have rung up a four and one record. Heart stopping wins in each of their last two matches against the Warriors and Manly has seen them become the comeback kids of the competition. The Tigers, much like the Panthers, appear to have wonderful depth this season, however that depth is set to be tested this week with Blake Austin and Kurtis Rowe both earning call ups to the NRL. Their spots will be taken here by Jy Hitchcox and Jack Madden, who both played plenty of matches for the Tigers in 2013, so chemistry should not be an issue. No such dramas for the home team who will arguably field their strongest team to date.
Summary: A winless start to the season almost came to an end for the Bluebags last round however a late Tyrone Phillips try to the Bulldogs has meant that Newtown head into this match with their backs against the wall. Despite this, the Jets will not be hitting the panic button just yet as a win here could possibly see them in the eight by rounds end, such has been the closeness of the competition over the opening rounds. Wentworthville, like Newtown, appeared headed for a draw last weekend until a Luke Kelly penalty goal after the siren got them out of jail against a resilient Wyong outfit. The Newtown Jets have had the wood on Wenty in recent years, including taking all three of the fixtures played between the two clubs last season which included knocking Wenty out in week one of the finals. This is a fact that would not have been lost on Brett Cook at training this week and we are sure he has reminded his players. Conversely, the home side will be feeling good about their chances against a side that they know they play well against. Joseph Ualesi is out here for Wenty and his place will be taken by club stalwart, Danny Howard. For the Jets, Paul Rokolati comes in for Jonathan Reuben while young Jake Watts has been named to make his club debut at this level and should offer the Jets some impact off the bench.
Summary: While the performance of their first grade side at Remondis Stadium last Saturday has garnered all of the headlines this week, we cannot overlook that the 44-6 win by the Warriors over Cronulla in the VB NSW Cup match was the first in the clubs short existence. Coach Rohan Smith will be a relieved man to have broken the duck after they went very close against the Cutters and Tigers in recent weeks, and he can now focus on taking his side to even loftier heights. The Bulldogs managed to earn themselves a draw last round when a late try to Tyrone Phillips was enough to see the Bulldogs take a point away from their trip to Henson Park. The Bulldogs still appear to be a bit of a work in progress under new coach Andrew Patmore, but there is far too much talent in their squad for them not to be winning games on a consistent basis, and one gets the feeling that once they click, the other sides in the competition will need to look out. Feleti Mateo and Chad Townsend have both been named here following the appointment of Andrew McFadden to the NRL coaches' hot seat mid-week while for the Dogs, their actual starting side may not be known right up until kick off with the NRL side naming an extended bench, and some other key players possible late inclusions. This match will be the first of three grades between the two clubs on Sunday from Eden Park, and with all matches shown live both in NZ and into Australia, we can't think of better way to start your Sunday.
Summary: The Sharks have lost three games in a row for the first time since early in the 2012 season and will head to Brooky to face a desperate Manly side who are still searching for their first win of 2014. Some close calls of late will give Manly fans some genuine belief that they can get the job done here against a Sharks side that is clearly down on confidence at present. When you cast your eyes across the Sharks roster, they certainly don't lack for talent, but unlike last year when they swept all before them, the chemistry between a number of new recruits just doesn't appear to be there just yet. On the flipside, Manly's chemistry has clicked in to gear somewhat in recent rounds but they just keep getting pipped at the post. The form of new recruit Manaia Rudolph has been encouraging and he will continue to grow in confidence the more he plays with his new team mates. The inclusion of NRL regulars such as Cheyse Blair and Tom Symonds will have also given this Manly side a massive confidence boost. Matt Prior has been named at lock here for the Sharks and they will also debut their midweek signing from the PNG Hunters, Mark Mexico. Our spies up north tell us Mexico has been in top form in the Intrust Super Cup, so it will be interesting to monitor his form over the coming weeks.
Summary: After the Bears round one 34-6 win over Wyong, not many would have figured that the Roos would be in eighth place and the Bears in ninth when they met again this round… But that is exactly what has happened. Since that round one win, the Bears have failed to post another victory while the Roos have managed to post two wins and perhaps could count themselves a shade unlucky that they didn't come away from Ringrose Park with at least a point last weekend. In the Bears defence, there have been some issues including injury at South Sydney over the past month that has definitely not helped their cause of late, but Wayne Lambkin will not want to use that as an excuse and will be hopeful of getting back on track here. Beau Champion and Kane Morgan have been named here for North Sydney after missing last week's loss to the Cutters while for Wyong, they have named an unchanged line up from the one that came so close against Wenty last week.


text:
package gobblin.compliance;

import com.google.common.base.Optional;
import com.google.common.base.Throwables;
import com.google.gson.JsonParseException;
import com.google.gson.JsonParser;
import lombok.extern.slf4j.Slf4j;

/**
 * Each Hive Dataset using gobblin-compliance for their compliance needs, must contain a dataset.descriptor property
 * in the tblproperties of a Hive Dataset.
 *
 * A dataset.descriptor is a description of a Hive dataset in the Json format.
 *
 * A compliance field is a column name in a Hive dataset to decide which records should be purged.
 *
 * A dataset.descriptor must contain an identifier whose value corresponds the column name containing compliance id.
 *
 * Path to the identifier must be specified in the job properties file
 * via property dataset.descriptor.identifier.
 *
 * Example : dataset.descriptor = {Database : Repos, Owner : GitHub, ComplianceInfo : {IdentifierType : GitHubId}}
 * If IdentifierType corresponds to the identifier and GithubId is the compliance field, then
 * dataset.descriptor.identifier = ComplianceInfo.IdentifierType
 *
 * @author adsharma
 */
@Slf4j
public abstract class DatasetDescriptor {
  protected String descriptor;
  protected Optional<String> complianceFieldPath;

  public DatasetDescriptor(String descriptor, Optional<String> complianceFieldPath) {
    checkValidJsonStr(descriptor);
    this.descriptor = descriptor;
    this.complianceFieldPath = complianceFieldPath;
  }

  protected void checkValidJsonStr(String jsonStr) {
    try {
      new JsonParser().parse(jsonStr);
    } catch (JsonParseException e) {
      log.warn("Not a valid JSON String : " + jsonStr);
      Throwables.propagate(e);
    }
  }

  public abstract String getComplianceField();
}

redpajama_set_name: RedPajamaGithub


text:
Priority Reports
BRCA1 Mutation Analysis of 41 Human Breast Cancer Cell Lines Reveals Three New Deleterious Mutants
Fons Elstrodt, Antoinette Hollestelle, Jord H.A. Nagel, Michael Gorin, Marijke Wasielewski, Ans van den Ouweland, Sofia D. Merajver, Stephen P. Ethier and Mieke Schutte
Fons Elstrodt
Antoinette Hollestelle
Jord H.A. Nagel
Michael Gorin
Marijke Wasielewski
Ans van den Ouweland
Sofia D. Merajver
Stephen P. Ethier
Mieke Schutte
DOI: 10.1158/0008-5472.CAN-05-2853 Published January 2006
Germ line mutations of the BRCA1 gene confer a high risk of breast cancer and ovarian cancer to female mutation carriers. The BRCA1 protein is involved in the regulation of DNA repair. How specific tumor-associated mutations affect the molecular function of BRCA1, however, awaits further elucidation. Cell lines that harbor BRCA1 gene mutations are invaluable tools for such functional studies. Up to now, the HCC1937 cell line was the only human breast cancer cell line with an identified BRCA1 mutation. In this study, we identified three other BRCA1 mutants from among 41 human breast cancer cell lines by sequencing of the complete coding sequence of BRCA1. Cell line MDA-MB-436 had the 5396 + 1G>A mutation in the splice donor site of exon 20. Cell line SUM149PT carried the 2288delT mutation and SUM1315MO2 carried the 185delAG mutation. All three mutations were accompanied by loss of the other BRCA1 allele. The 185delAG and 5396 + 1G>A mutations are both classified as pathogenic mutations. In contrast with wild-type cell lines, none of the BRCA1 mutants expressed nuclear BRCA1 proteins as detected with Ab-1 and Ab-2 anti-BRCA1 monoclonal antibodies. These three new human BRCA1 mutant cell lines thus seem to be representative breast cancer models that could aid in further unraveling of the function of BRCA1. (Cancer Res 2006; 66(1): 41-5)
Gene mutation
Germ line mutations of the BRCA1 breast cancer susceptibility gene predispose female carriers to develop breast cancer and ovarian cancer (OMIM 113705; http://www.ncbi.nlm.nih.gov/omim/). The BRCA1 protein normally resides in a nuclear multiprotein complex, including BRCA2, BARD1, and RAD51, and the DNA damage repair proteins MSH2, MLH1, MSH6, ATM, NBS1, MRE11, RAD50, BLM, and RFC. This BRCA1-associated genome surveillance complex functions as a sensor of abnormal DNA structures, such as double-strand breaks and base pair mismatches. BRCA1 has been suggested to have a pivotal function within BRCA1-associated genome surveillance complex by coordinating the actions of damage-sensing proteins and executive repair proteins. BRCA1 may also act as a transcriptional regulator of genes involved in checkpoint reinforcement and, in complexes with BARD1, as a ubiquitin ligase (reviewed in refs. 1– 4). Thus, mutations of BRCA1 likely impair the repair of damaged DNA, thereby rendering the mutant cells prone to malignant transformation. To fully unravel the function of BRCA1 in DNA damage responses, cell lines with naturally occurring mutations of the gene provide invaluable research tools as they allow extensive analyses and in vitro manipulation. Only a single human BRCA1 mutant breast cancer cell line had thus far been described (HCC1937; ref. 5). To identify additional mutants, we screened 41 human breast cancer cell lines for alterations in the BRCA1 gene sequence.
Breast cancer cell lines. The 41 human breast cancer cell lines used in this study are listed in Table 1 . The SUM series were generated in the Ethier laboratory (available at http://www.asterand.com). Cell lines EVSA-T, MPE600, and SK-BR-5/7 were kind gifts of Dr. N. DeVleeschouwer (Institut Jules Brodet, Brussels, Belgium), Dr. H. Smith (California Pacific Medical Center, San Francisco, CA), and Dr. E. Stockert (Sloan-Kettering Institute for Cancer Research, New York, NY), respectively. Cell line OCUB-F was obtained from Riken Gene Bank (Tsukuba, Japan). All other cell lines were obtained from American Type Culture Collection (Manassas, VA). Extensive analysis of nearly 150 polymorphic microsatellite markers had shown that all cell lines are unique and monoclonal ( 6).
BRCA1 mutation analysis of 41 human breast cancer cell lines
Mutation analysis. The complete coding sequence and exon-intron boundaries of BRCA1 (Genbank U14680) were analyzed for genetic alterations in all cell lines, except for SUM44PE and ZR75.30 (only exons 3-7 and 11-15 were analyzed, respectively). Exons 1a to 11 and 16 to 24 were PCR amplified from genomic DNA templates and exons 12 to 15 were amplified from RNA templates, as described ( 7). Amplification products were then analyzed for sequence alterations with the Big Dye Terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA) using an ABI 3100 Genetic Analyzer. All unique sequence alterations were confirmed by sequencing of an independently amplified template. This approach may allow mutations to go undetected in cell lines without allelic loss, specifically deletions when analyzing DNA and truncating mutations that result in down-regulated transcripts when analyzing RNA. Allelic loss of the BRCA1 gene was determined by PCR-based microsatellite analysis as previously described ( 6). BRCA1 and HPRT transcripts were concurrently amplified from RNA templates, using the Qiagen Onestep reverse transcription-PCR (RT-PCR) kit and gene-specific primers. Primer sequences are available upon request.
Immunocytochemistry. Cell lines were cultured to optimal cell density in eight T162 flasks and medium was refreshed 24 hours before harvesting. Cells were harvested by scraping, washed twice with PBS, and fixed in PBS with 2% formalin for 12 to 72 hours. Cells were then washed once with PBS, resuspended in liquidized PBS with 2% agarose, and embedded in paraffin by routine diagnostic procedures. Paraffin sections (4 μm) on Starfrost microscope slides (Knittel Gläser, Braunschweig, Germany) were routinely deparaffinized and dehydrated, and epitopes were retrieved in Tris-EDTA (pH 9.0) for 30 minutes at 100°C in a microwave oven. Slides were blocked with 2% bovine serum albumin in PBS for 30 minutes at room temperature and then incubated overnight at 4°C with antibodies diluted in Normal Antibody Diluent (Scytek Laboratories, Logan, UT). Anti-BRCA1 mouse monoclonal antibodies Ab-1 (clone MS110; 1:100 or 1 μg/mL) and Ab-2 (clone MS13; 1:320 or 0.6 μg/mL) were both purchased from Calbiochem (Darmstadt, Germany) and isotype-matched control monoclonal antibody X0931 (1:100 or 1 μg/mL) from Dako (Glostrup, Denmark). Slides were developed using the DakoCytomation Envision System horseradish peroxidase (3,3′-diaminobenzidine) kit, with omission of the antiperoxidase treatment. Slides were counterstained for 5 seconds with hematoxylin. Both anti-BRCA1 antibodies were titrated in two-step serial dilutions on BRCA1 wild-type cell lines. At the presumed optimal antibody dilution, both antibodies showed distinct nuclear staining and Ab-1 also gave slight cytoplasmic staining. More diluted antibodies showed only nuclear staining for both antibodies and less diluted antibodies were aspecific (examples of wild-type and mutant cell lines at several antibody dilutions are provided as Supplementary Data).
Sequencing of BRCA1 revealed 18 different alterations in the gene sequence among 41 human breast cancer cell lines ( Tables 1 and 2 ). Alterations were presumed to be nonpathogenic polymorphisms when they were described as such in the Breast Cancer Information Core (BIC) mutation database (http://research.nhgri.nih.gov/bic/). Together, 11 BRCA1 polymorphisms were identified among 29 of the breast cancer cell lines. Three other BRCA1 variants had been described as unclassified variant in the BIC mutation database and were each detected once in the cell lines (788 + 3G>A in MDA-MB-330 and S1140G, and 5106-20A>G both in UACC812). Deleterious BRCA1 mutations were identified in four breast cancer cell lines ( Tables 1 and 2). The insertion of a cytosine residue at position 5382 of BRCA1 in cell line HCC1937 had been reported ( 5). In cell line MDA-MB-436, we identified the 5396 + 1G>A mutation in the splice donor site of exon 20 ( Fig. 1 ). Analysis of BRCA1 transcripts from MDA-MB-436 identified two transcript lengths. Sequencing revealed that one transcript had skipped exon 20, predicting an in-frame deletion of 28 amino acids in the encoded BRCA1 proteins, whereas the other transcript had spliced at a cryptic splice site in intron 20 (5396 + 88/89), predicting an insertion of seven amino acids encoded by intron sequences followed by a termination codon. The patient from whom MDA-MB-436 was generated had been diagnosed with adenocarcinoma of the breast at age 39 ( 8), an early onset that is suggestive for hereditary breast cancer. The original tumor was not available for analysis but the 5396 + 1G>A mutation has been reported 46 times in the BIC mutation database and is classified as pathogenic. In cell line SUM149PT, we identified the deletion of a thymine residue at position 2288 of BRCA1 ( Fig. 1). The 2288delT mutation predicts a shift in the BRCA1 reading frame with an insertion of 12 new amino acids after codon 723 followed by a termination codon. The patient was diagnosed at age 35 years with inflammatory breast carcinoma and she had a single known second-degree relative with postmenopausal breast cancer. The 2288delT mutation was not present in the germ line of the patient as we did not detect the mutation in a DNA sample from her blood. Of note, the identity of the donor was confirmed by analysis of 10 microsatellite markers from three chromosomes, with heterozygosity ratios of >0.80 for all markers (P < 10−7). The original tumor was not available for analysis but the 2288delT mutation was detected in all available passages of the SUM149 cell line. It is thus unclear whether the mutation was somatically acquired during tumorigenesis in the patient or in vitro during establishment or propagation of the SUM149 cell line. Importantly, we detected the 2288delT mutation in the earliest available passage P16 and cells were only distributed to other laboratories after this passage. We identified an AG dinucleotide deletion at position 185 of BRCA1 in cell line SUM1315MO2, predicting a shift in the BRCA1 reading frame with an insertion of 16 new amino acids after codon 22 followed by a termination codon ( Fig. 1). The patient was diagnosed with invasive ductal carcinoma of the breast but the age at diagnosis nor the cancer history of her family is known. The original tumor was not available for analysis but the 185delAG mutation is a well-described pathogenic BRCA1 mutation that is prevalent in the Ashkenazi Jewish population (http://research.nhgri.nih.gov/bic/).
BRCA1 sequence variants among 41 human breast cancer cell lines
Identification of three new BRCA1 mutant breast cancer cell lines by PCR amplification and direct sequencing. Top, electropherograms displaying the wild-type sequence. Bottom, electropherograms displaying the mutations.
Allelic loss of the BRCA1 gene was determined by PCR amplification of microsatellite markers D17S1321, D17S932, D17S855, D17S1327, and D17S1325. These markers are located within a 0.7 Mb chromosomal region encompassing the BRCA1 gene at 17q21. Analysis of the markers on germ line DNAs from 25 randomly selected Dutch individuals revealed heterozygosity ratios of 0.61, 0.76, 0.60, 0.55, and 0.88, respectively. Allelic loss of the BRCA1 locus was presumed when each of the five markers had a single allele size, resulting in a reliability of P = 0.002 ( 6). None of the 25 control DNAs had a homozygous allele pattern at the BRCA1 locus, thus validating this statistical approach. Of the 41 breast cancer cell lines, 28 (68%) had allelic loss of the BRCA1 locus, including the four BRCA1 mutants ( Table 1). Similar allelic loss frequencies have been reported for primary breast cancer specimens ( 9– 11). It is important to note that several regions at 17q are frequently amplified in human breast cancers. Allelic losses at 17q are therefore often underestimated, as karyotype-based methods do not detect loss when the retained allele is amplified or reduplicated ( 6, 12). Indeed, we did not identify loss of the BRCA1 locus in three BRCA1 mutant cell lines that we analyzed by array comparative genome hybridization (data not shown), whereas our microsatellite analysis revealed allelic loss in all of them. Conclusively, all BRCA1 mutants were homozygous in the sequence analysis ( Fig. 1).
BRCA1 transcript expression was analyzed by semiquantitative RT-PCR using five overlapping PCR fragments ( Table 1). Cell lines HCC1937 and MDA-MB-436 had BRCA1 transcript expression levels that were comparable with those of most other cell lines, SUM149PT had variable but always lower expression levels, and SUM1315MO2 had consistently low expression of BRCA1 transcripts. In contrast with wild-type cell lines, nuclear BRCA1 protein expression was not detectable in any of the four mutant cell lines, as determined by immunocytochemistry on paraffin-embedded cells using anti-BRCA1 monoclonal antibodies Ab-1/MS110 and Ab-2/MS13 ( Fig. 2 ; Supplementary Data).
BRCA1 immunocytochemistry in BRCA1 mutant and wild-type breast cancer cell lines. In contrast with the two wild-type cell lines (BT20 and SK-BR-7), none of the four BRCA1 mutants had nuclear BRCA1 staining with either of the two anti-BRCA1 monoclonal antibodies Ab-1 and Ab-2. There is some cytoplasmic staining of unclear significance in all samples with Ab-1, which is not observed with more diluted Ab-1 antibodies nor with Ab-2 (see also Supplementary Data). The negative control antibody is an IgG1 isotype-matched antibody. Magnification, ×40.
We thus describe four cell lines with a BRCA1 mutation from among 41 human breast cancer cell lines, three of which had not previously been reported. All BRCA1 mutants had lost the other BRCA1 allele, in accordance with the tumor suppressor function of BRCA1. Three mutations generated a premature termination codon in the BRCA1 transcript, whereas the fourth mutation resulted in two transcripts of which one had an in-frame deletion and the other generated a premature termination codon. Three of the BRCA1 mutations have been classified as pathogenic mutations and none of the BRCA1 mutant cell lines expressed nuclear BRCA1 proteins. In an ongoing effort to characterize our panel of breast cancer cell lines, we identified biallelic mutations of the p53 tumor suppressor gene in each of the four BRCA1 mutants, 5 consistent with the notion that BRCA1 mutant tumors frequently harbor p53 mutations (reviewed in ref. 13). Pending further mutational data, these BRCA1 mutant breast cancer cell lines already are a valuable asset in pinpointing the BRCA1 functions that are critical in the suppression of breast tumorigenesis.
Grant support: Dutch Cancer Society.
We thank Hans Stoop and Mieke Timmermans for technical advise regarding BRCA1 immunocytochemistry.
Note: Supplementary data for this article are available at Cancer Research Online (//cancerres.aacrjournals.org/).
↵5 M. Wasielewski et al., submitted for publication.
Received August 11, 2005.
Revision received October 21, 2005.
Scully R, Livingston DM. In search of the tumour-suppressor functions of BRCA1 and BRCA2. Nature 2000; 408: 429–32.
Venkitaraman AR. Cancer susceptibility and the functions of BRCA1 and BRCA2. Cell 2002; 108: 171–82.
Tutt A, Ashworth A. The relationship between the roles of BRCA genes in DNA repair and cancer predisposition. Trends Mol Med 2002; 8: 571–6.
Starita LM, Parvin JD. The multiple nuclear functions of BRCA1: transcription, ubiquitination and DNA repair. Curr Opin Cell Biol 2003; 15: 345–50.
Tomlinson GE, Chen TT, Stastny VA, et al. Characterization of a breast cancer cell line derived from a germ-line BRCA1 mutation carrier. Cancer Res 1998; 58: 3237–42.
Harkes IC, Elstrodt F, Dinjens WN, et al. Allelotype of 28 human breast cancer cell lines and xenografts. Br J Cancer 2003; 89: 2289–92.
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Fukino K, Iido A, Teramoto A, et al. Frequent allelic loss at the TOC locus on 17q25.1 in primary breast cancers. Genes Chromosomes Cancer 1999; 24: 345–50.
Shen CY, Yu JC, Lo YL, et al. Genome-wide search for loss of heterozygosity using laser capture microdissected tissue of breast carcinoma: an implication for mutator phenotype and breast cancer pathogenesis. Cancer Res 2000; 60: 3884–92.
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Gasco M, Yulug IG, Crook T. TP53 mutations in familial breast cancer: functional aspects. Hum Mutat 2003; 21: 301–6.
Esteller M, Silva JM, Dominguez G, et al. Promoter hypermethylation and BRCA1 inactivation in sporadic breast and ovarian tumors. J Natl Cancer Inst 2000; 92: 564–9.
You are going to email the following BRCA1 Mutation Analysis of 41 Human Breast Cancer Cell Lines Reveals Three New Deleterious Mutants
Cancer Res January 1 2006 (66) (1) 41-45; DOI: 10.1158/0008-5472.CAN-05-2853
ATM Loss and Therapeutic Sensitivities in Prostate Cancer
Multimodal Molecular Imaging of the Tumor Microenvironment
Contribution of EMT Mechanism in Breast Tumor Metastasis
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