Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats.
Home » Topics » Bioinformatics » Research » Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats.
Summary of "Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats."
The genome editors CRISPR/Cas9 (clustered regularly interspaced short palindromicrepeats/Cas9 nuclease-null) and TALENs (transcription activator-like effector nuclease) are popularly used for targeted modification of the mammalian genome. To date, few comparative studies have been carried out to investigate the differences between the use of CRISPR/Cas9 and TALENs in genome editing for goat breeding. Here, we compared CRISPR/Cas9 and TALEN technologies at multiple levels for generating a knock out (KO) of the Alpas cashmere goat myostatin (MSTN) gene, which negatively regulates the proliferation and differentiation of skeletal muscle cells. The electrotransfection efficiency observed using CRISPR/Cas9 was 8.1% more than that observed using TALEN for generating MSTN KO cells. In addition, the cutting efficiency of CRISPR/Cas9 for editing exon 1 of the MSTN gene was higher than that of TALENs. However, the off-target effects of the CRISPR/Cas9 system were also higher than those of TALENs. Further, we found that the frequency of obtaining MSTN mutations by CRISPR/Cas9 was 8.5 times higher than that by TALEN. The CRISPR/Cas9-edited colonies involved longer deletions (up to 117 bp) than the TALEN-edited colonies (up to 13 bp). Remarkably, when embryos used to generate cloned goat via somatic cell nuclear transfer were compared, we found that the TALEN MSTN KO embryos easily developed to 8 cells and their cleavage rate was significantly higher than that of CRISPR/Cas9-edited embryos. Finally, we produced a MSTN KO lamb using CRISPR/Cas9, which suggested that a high level of targeted gene modification could be achieved in goat using CRISPR/Cas9. Taken together, our study indicates that although TALEN enables a variety of genome modifications and may have some advantages over CRISPR/Cas9, the latter provides a significant advantage by permitting precise and efficient gene editing. Thus, CRISPR/Cas9 has more potential to become a robust gene-engineering tool for application in the breeding of farm animals.
Mosaicism in CRISPR/Cas9-mediated Genome editing.
CRISPR-Cpf1-mediated genome editing and gene regulation in human cells.
Visual Assay for Gene Editing Using a CRISPR/Cas9 System in Carrot Cells.
The different gene transcripts generated from a single gene by RNA EDITING or ALTERNATIVE SPLICING of RNA PRECURSORS.


For some reason we tend to avoid asking for help. It is one of those things we are all guilty of doing. On multiple occasions when I needed help, I shied away from asking for it. The reasons vary situation to situation; sometimes we do not want to ask for help because it is an ego issue, other times we are just too ashamed to ask for it and sometimes there is no one to ask.


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Give an answer: 7+8 = ?


Clam Beach Park is located on US-101 just north of Arcata, CA. It's operated by Humboldt County and provides a place to camp near the beach. Not much to it, but we enjoyed it for its view of the ocean and bright sunshine.
We arrived late on a Friday morning, concerned about whether it would be full for the weekend. We needn't have worried - plenty of room in the parking lot that serves as a campground. We chose site 19, for it was at the end of the row next to the handicapped space, so we'd be less likely to have neighbors close by on each side .
This shows just about the entire parking lot. There are 31 parking spaces; some are for use by tenters. The spaces are pretty narrow, so your neighbors will be awfully close when it's busy here.
By the next morning we had gathered a few neighbors.
Not much in the way of amenities here - just a vault toilet, and there is a water faucet outside.
The campground is at the northern terminus of the Hammond Trail - a hike/bike/horse trail that leads back to Arcata.
From what I've read, Clam Beach is one of the very few Pacific beaches on which vehicles are allowed.
The sand is lot softer than places like Daytona Beach or St Augustine Beach in Florida where cars on the beach are quite common.
Sur two-night stay was well worth it in terms of entertainment! All sorts of interesting characters showed up here. one was this fellow living in an old step-van. He spent the entire afternoon cleaning it out. The best, though, was when he brought out one of those singing fish plaques and tried to figure out how to hang on the side of the van. We loved the songs it played: "Don't worry, be happy" and "Take me down to the river, throw me in the water.".
This crew pulled in, much to the host's dismay. A guy and a gal in the lead truck, with another woman in the little truck to brake and steer. Her skills were marginal, as she nearly smashed into the back of the green truck when they stopped. The fellow tried some repairs to the truck, to no avail, and half an hour later, towed it out.
Too bad she doesn't show up better, but this lady pulled in for a few minutes. Seemed to be well into her 70's, had a large white dog for a traveling companion, and smoked cigars! Quite the jaunty straw hat, too.
This gang arrived by taxi! Four of them with huge packs and four dogs. A few days later we encountered them a little further up the road.


We all love monsoon and we wait for the rainy season after going through the scorching summers. The first downpour truly feels like a sigh of relief after the crazy hot summers.
Monsoon is definitely the best time to enjoy hot tea and pakoras, but this is also that time of the year when infectious diseases are on the ascent.
The cool downpour of the monsoon, brings with it negative health impacts along with it which we need to safeguard ourself from.
We are aware of a lot of health issues and infections that are brought about by monsoons and we ought to be very careful to protect ourselves from.
Below I have listed a few precautionary health measures which one can take to avoid any kind of health hazards/ infections and also mentioned some food changes that can be made for a healthier monsoon.


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Iran says tanker freed by Gibraltar poised to set sail
By AFP Aug 16, 2019 in World
A tanker carrying Iranian oil, released by Gibraltar authorities after being held since July 4, is preparing to set sail into the Mediterranean, a senior Iranian shipping official said Friday.
The ship's seizure, with the help of British Royal Marines, had triggered a sharp deterioration in relations between Tehran and London and the tit-for-tat detention by Iran of the British-flagged tanker Stena Impero.
The Grace 1 will be renamed and switch to the Iranian flag for its onward journey, the deputy head of Iran's Ports and Maritime Organisation, Jalil Eslami, told state television.
"At the owner's request, the Grace 1 will depart for the Mediterranean after being reflagged under the Islamic Republic of Iran's flag and renamed as Adrian Darya for the voyage," Eslami said.
"The ship was of Russian origin and Panama-flagged and is carrying two million barrels of Iranian oil," he added.
Gibraltar's Supreme Court ordered the tanker released on Thursday after the British overseas territory said it had received written assurances from Iran that the Grace 1 would not head to any country subject to European Union sanctions.
The ship had been detained on suspicion that its cargo was destined for the Banias oil refinery in Syria in breach of an EU embargo.
But Iran denied it had provided any assurances to secure the ship's release, saying Gibraltar was only seeking to "save face".
"Iran has given no assurances over the Grace 1 not going to Syria to secure its release," the state broadcaster's youth website quoted foreign ministry spokesman Abbas Mousavi as saying Friday.
"The tanker's destination was not Syria ... and even if it was, it did not concern anyone else."
Hours before the court's ruling, the administration of US President Donald Trump, which has waged a campaign of "maximum pressure" against Iran, launched a last-minute legal move demanding that the Gibraltar authorities extend the vessel's detention.
Iranian Foreign Minister Mohammad Javad Zarif tweeted that the US attempt at "piracy" had failed, saying it showed the Trump administration's "contempt for the law".
Tehran and Washington have been at loggerheads since Trump withdrew from a landmark 2015 nuclear deal between major powers and Iran last year and reimposed crippling unilateral sanctions.
Following the Grace 1's release, Britain renewed its demand that Iran release the British-flagged tanker it seized in the Strait of Hormuz on July 19.
Tehran charged that Stena Impero was in violation of "international maritime rules" but the move was widely seen as retaliation for the detention of the Grace 1.
More about Diplomacy, Oil, Iran, Britain, Gibraltar
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Shop Bonobos
Pose's Johnny Sibilly on the Ballroom Scene, Representation, and Style
By Amelia Diamond
Johnny Sibilly hasn't won an Emmy — yet. The actor, who plays Costas Perez on FX's Pose, hasn't been nominated, either (yet), but he is currently under Emmy consideration, and in his eyes, that's a special honor unto itself.
"For the network to say, 'We believe in you enough to submit you [for an Emmy nomination],' I mean, that's all you can ask for as an actor. It feels so validating. Especially because I'm playing the role of a queer, Dominican man who is dying of AIDS, and that is so important to me. Getting to play the role alone feels like a win."
Pose, the history-making television show about New York City's underground ball culture — a show that "features the largest cast of transgender actors in series regular roles," as well as "the largest recurring cast of LGBTQ actors ever for a scripted series" — returned to FX for its Second Season. Just a few days before the June 11 launch and a few days after the season premiere, Bonobos sat down with this star-on-the-rise. Hold the "yet." Johnny Sibilly's here.
So much of storytelling is about using style to tell that story — especially with your characters. How do you use style to tell your story?
Johnny wears the Premium Stretch Jeans (All Black), the Classic Pique Polo (Blue Floral), and the Monochrome Leather Belt (Black)
My favorite thing is getting ready for something. I sometimes even shop in the women's section just to create something different. I do love a masculine look, but with a silk top, or a flow-y shirt, but then I love a polo and a fitted pant. I love to be able to play on the spectrum of both fashion and gender.
Growing up, everyone was like, "You have to pick. What's your style, are you a prep? Are you a—?" And I remember I was like, "No, one day I want to wear a jersey. And one day I want to wear a polo with puka shells," which I definitely did, often. Someone called me a chameleon once as an insult, and I was like, "Oh my God, I don't know who I am." Now I'm like: No, I'm a conglomerate of different things and experiences that I've had.
There's so much style within the ballroom scene that Pose portrays. Can we talk about that, about what it was like being surrounded by it on set, and its history?
The history is so important. My best friend, Trace Lysette, she's actually from the ballroom culture, ballroom scene. What a lot of people don't realize is that ballroom culture has been at the forefront of so many cultural revolutions in terms of style, and dance, and speech. Like the fact that we all say, "work"; Vogueing, the style of dance — it comes from [ballroom culture].
A lot of these people in the ballroom scene, you don't realize that over the years, they've been hairstylists, stylists, choreographers to the most famous people in the world, but they're always in the background. What's so great about a show like Pose is that they're giving those people who have been "style whisperers" the opportunity to be in the forefront of fashion and entertainment.
These are people on the margins, and have been for so many years, but they take whatever they can and they make it into something. So they can't afford [a designer gown], but they're going to give you a silhouette and make it look like [a designer gown], and they're going to sell it like that. That's why ballroom's so special, because it plays to the kid in all of us. It's like: you're not there yet, but that doesn't mean to say you can't act like you are.
Thanks to the show, and the videos you make and share across social media, you have this great platform. How do you hope to use your voice and platform for good?
[By] using my platform to just exist in a space where people like me don't often exist; making sure younger people know that you don't have to wait as long [to come out], and everyone's journey is different. That's also important to honor as well: not everyone is going to come out right away, but knowing and being that kind of picture for — especially for black and brown, Latino kids, who oftentimes are very scared because of their family dynamic and the way we're all raised — to see me. And to just be like, "Oh, she can do it, I can do it."
Also, I do a lot of the characters especially for the gay community to realize it's okay to not take ourselves seriously, to poke fun at ourselves and to enjoy our humor. Because I feel a lot of times we fight to be respected and to be honored in the society that we live in, but it's also important to just have fun and to know that we're special and goofy AF.
Do you have anything else that you'd like to share with us?
Tune in to Pose Season Two. Put your money where your mouth is. Support queer, black, brown, trans…all the people on the margins.
Trevor's a great place [to donate/get involved]. I'm currently working with Trevor Project [on their] Face Your Fears campaign. To be part of the Trevor Project, which reaches out to youth who are struggling to come out, or [who are] dealing with the demons they have, that means something.
Also, Google smaller organizations specifically to deal with whatever you fall in line with. There are resources that you can give money to that need it more so than the bigger corporations that are getting million dollar funding.
I listened to Dominique [Jackson] at the Pose screening and she said, "It's nice that you see us kiki-ing and having a good time, but you must remember that there are still black trans women out there who are being murdered just for existing. And while watching Pose is important, supporting and being an [ally] to the community is even more important."
Pose Season 2 airs Tuesday nights on FX. Click here for episodes and more info. Pose Season 1 is currently streaming live on Netflix. Follow Johnny Sibilly on Instagram @JohnnySibilly.
Amelia Diamond is a writer and creative consultant. Follow her on Instagram, @amilli0naire.
Photos by Liam Goslett
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American Idol 2012: Phillip Phillips Surgery Postponed Due to Fever!
American Idol 2012 winner Phillip Phillips was reportedly scheduled for vital kidney surgery on Tuesday, but it looks like he will have to wait a little longer before he goes under the knife. According to the latest reports, the Phillip Phillips surgery has been postponed because the American Idol winner is suffering from a high fever.
Phillips Phillips suffers from a congenital kidney disorder that creates huge kidney stones that cannot be naturally passed out of the body. The American Idol 2012 winner had to go through eight prior surgeries to relieve the extremely painful buildup of kidney stones during his time on season 11. This latest surgery will reportedly be to do repair work on his right kidney, which has been ravaged by his condition.
According to TMZ.com, Phillip Phillips' surgery, which was allegedly scheduled for Tuesday, has been postponed due to a high fever caused by a sinus infection. The kidney surgery has reportedly been rescheduled to take place next week and the estimated recovery time is 4 to 6 weeks. The American Idols Live tour launches on July 6 in Detroit. With the delay in his surgery, it is unknown at this point if he will be recovered enough to be present for the kick-off of the tour.
Meanwhile, Phillip Phillips has put off engaging in post-American Idol promotional duties, including scheduled appearances on the Today show and Live! With Kelly. He was also forced to pull out of several post-Idol promotional gigs, such as an appearance at the American Idol Experience attraction at Disney World.


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Ніна Борисівна Мечковська (,  ) — білоруська мовознавець, соціолінгвіст, дослідниця історії мови. Професор кафедри теоретичного та слов'янського мовознавства Білоруського державного університету (з 1989). Доктор філологічних наук (1987).

Життєпис
1968 року закінчила Білоруський державний університет, філологічний факультет, відділ російської мови та літератури за спеціальністю «філолог. Викладач російської мови та літератури, викладач англійської мови».

Викладацька діяльність закордоном::
 Рурский університет, Бохум (гостьовий професор Славістичного семінару; жовтень 1994 — лютий 1995);
 Люблянський університет (гостьовий професор Відділення слов'янських мов і літератур; жовтень-грудень 1996).

Досліджує такі галузі мовознавства: теорія комунікації, соціолінгвістика, психолінгвістика, історія літературних мов.

Лауреат Премії БДУ ім. академіка В. І. Пічети (2001). Читає курс загального мовознавства, курс лекцій для магістрантів «Методологія лінгвістичних досліджень», керує спецсемінаром «Психолінгвістика і психологія комунікації».

Член Білоруського комітету славістів і Міжнародної комісії зі слов'янських літературних мов при Міжнародному комітеті славістів. Член редколегій міжнародних наукових журналів «Русский язык в научном освещении» (Москва), «Russian Linguistics» (Дордрехт), «Slavistična revija» (Любляна).

Дисертації:
 «Морфолого-семантический очерк русской терминологии языкознания», 1971. Керівник — д.фл.н. професор Булахов М. Г. (кандидатська)
 «Концепции и методы грамматик VI–VII веков как элемента книжно-письменной культуры восточного славянства», 1987 (докторська)

Підготувала 23 кандидати наук.

Праці
Автор 13 книг і понад 300 публікацій.

Головні книги, збірки та посібники:
 Ранние восточнославянские грамматики (Мінськ, 1984)
 Словенский язык (Мінськ, 1991)
 Общее языкознание (кілька видань)
 Социальная лингвистика (кілька видань)
 Язык и религия (Москва, 1998)
 Сацыякультурная прастора мовы (Мінськ, 1998)
 Белорусский язык: Социолингвист. очерки (Мюнхен, 2003. Specimina philologiae slayicae; Band 138).
 Общее языкознание: структурная и социальная типология языков (кілька видань)
 Семиотика: Язык. Природа. Культура (Москва, 2004)
 Мовы і культура Беларусі (Мінськ, 2008)
 История языка и история коммуникации: от клинописи до Интернета (Москва, 2009)

Джерела
 Візитівка на сторінці кафедри теоретичного та слов'янського мовознавства
 Профіль на сторінці університету

Білоруські мовознавці
Соціолінгвісти
Випускники Білоруського державного університету

redpajama_set_name: RedPajamaWikipedia


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83-й выпуск  Гент — Вевельгем — шоссейной однодневной велогонки по дорогам Бельгии. Гонка прошла 28 марта 2021 года в рамках Мирового тура UCI 2021. Победу одержал бельгийский велогонщик Ваут Ван Арт.

Участники
Автоматически приглашения на гонку получили 19 команд категории UCI WorldTeam и лучшая UCI ProTeam команда прошлого сезона Alpecin-Fenix. Также организаторы пригласили ещё 5 команд категории ProTeams.

Однако незадолго до старта гонки с неё в полном составе снялась команда Trek-Segafredo из положительного теста на COVID-19 у одного из членов её команды. А команде Bora-Hansgrohe запретили стартовать из-за обязательного недельного карантина её членов после положительного теста на COVID-19 полученного несколькими днями ранее перед гонкой E3 Саксо Банк Классик Таким образом в гонке приняло участие 23 команды.

Маршрут

Ход гонки
Ещё до начала гонки из-за сильного ветра (7,5 м/с) предполагалось, что гонка сильно растянется. И ветер действительно сыграл свою роль — уже в начале гонки пелотон был разбит на несколько групп.

За 97 километров до финиша определилась группа в 25 гонщиков, среди которых были Сэм Беннетт, Данни Ван Поппель, Сонни Кольбрелли. Отрыв был достаточно резвым, и многие команды его пропустили, тем не менее Зденек Штыбар, Давиде Баллерини и ещё двое гонщиков попытались броситься в погоню.

При преодолении второго подъёма в лидирующей группе осталось 9 спортсменов.

За 16 километров до финиша несколько гонщиков первой группы предпринимали попытки оторваться, но тот же Беннетт уже не мог составить конкуренцию из-за плохого самочувствия.

За 9 километров до финиша идущие во главе группы отрыва стали оборачиваться в ожидании атак снизу.

За километр до финиша раскачивать группу начал Натан Ван Хойдонк, но жертвуя собой ради лидера команды, он ушёл с общей траектории, где, взяв левее, начал спринтовать Штефан Кюнг с Майклом Мэттьюсом на колесе, а Ваут Ван Арт, идя третьим, начал подтягиваться к центру дороги, и, блокируя Кольбрелли, финишировал первым. Таким образом, Ваут Ван Арт стал пятидесятым бельгийцем, победившим на Гент — Вевельгем.

Результаты

Примечания

Ссылки
 Официальный сайт


This week, the Top 5 contestants get tested on their physical strengths, coordination and their ability to present while coping with physical adventures.
So far we've tested their styling skills, organisational skills, celebrity interviewing skills, MC'ing and interactions with the audience; now comes the adventure challenges and physical tests.
Join us this week as the Top 5 draw their unique physical challenges out of the Top Hat, where we'll see how they cope with the physical tasks while still maintaining their Top Billing poise.
The main aim of the task is to test their physical strengths, coordination and their ability to present while coping with physical adventures.
Tune in to catch all the drama and excitement of the My Top Billing Dream Reality Show week 4! This is definitely not a show you want to miss!


Se fini!!! I got my Student Visa today!! I can now go to France legally for one year!!! hurrah!!!
Getting it has been quite the process. Check out Part 1 for the full scoop. The full process (minus getting my acceptance letter from Le Cordon Bleu has taken about a month). A lot of the time was spent gathering paperwork or waiting. Not too bad but you definitely have to be organized!
Step #4: Wait for your confirmation email from Campus France. It says congratulations! in the email and you'll only see it come through in your Campus France email. They said to allow a 2-3 week turn around from sending in your information. Mine only took a week!
Step #5: Sign up for an interview at your closest French consulate. Mine's in Houston a 3.5 hour drive from Dallas. yeehaw! haha There was actually a TON of availability. I was so worried that I would have to schedule it several weeks out. I'm sure this as well as the time for Campus France to review your paperwork is dependent on the season.
My Passport w/ at least 2 Visa pages blank and issued less than 10 years ago and valid for more than 3 months from when I get back.
Processing Fee. For a long term student visa the fee is only 50 euros. I was totally expecting 100-300 euros. It can be paid in dollars, with a credit card or cash.
Residency Form (This actually isn't saying where I'll be living when I get to France, but rather a precursor to the final validation step I'll have to do once I'm in France).
Financial Guarantor. Either a note from your parents saying they will be sending $820/month or proof of income while abroad.
Self-Addressed Prepaid envelope to send your passport/visa back to you!
What was the interview like you ask? Well the two things above should give you an idea. I was SUPER nervous the night before because getting my visa makes or breaks this whole thing! I had read to look nice so I was fretting over what to wear till midnight- and the fact that it was going to be about 50 degrees didn't make it any easier to decide. I got up at 5:30am and was out the door by 6- brutal.. The drive was brutal. I got there about 45 minutes before my appointment and figured out which office building it was in and settled down in the Starbucks across the street to wait.
15 minutes before I headed to my car- off to find a parking spot and get to the office right on time. Well parking is a little tricky- It's around back in a parking garage. Not bad but added a little extra time. I took the elevator up, again shaking in my boots with all my paperwork. Stepped through the door indicating Visas and it looked like the DMV. Why had I stayed up so late to pick out what to wear?! The ladies behind the glass were helping someone so I put my appointment slip under the glass and took a seat (as indicated by the sign). 40 minutes later… quite a while after the person they were helping had left, and they still hadn't called my name. The glass was see through (I checked!) and they saw me come in! I'm not one to mess with the process especially if it's something as super official as this so I waited. and waited. Another person came in and walked straight to the window. Then they happened to see that someone was sitting in the waiting area. About 15 minutes later I was ushered over.
The ladies running the show were very French. One was wearing a Cowboy belt buckle and ties though. 🙂 If you speak French, now would be the time to pull it out. I was only able to muster a "Bonjour" without getting intimidated. They do speak English but it's not as well understood as if you were speaking French. The "interview" consisted of passing through the appropriate documents. I thought I was good until- "Where's your apartment lease?" uh I don't have it with me? "Well we'll need your apartment lease" ok. I can send it to you. "How long is your lease for?" 3 months I think? "Well it has to be for 6 months." I booked it online and we want to find our next apartment when we get there so we can check the location and such. "I still need a lease for 6 months." Can I get the landlady to write a letter saying I could extend it to 6 months? "That might work.." ugh. Then the next question. "Do you have your Health insurance?" No… "You are not covered by the national system if you're going to the Cordon Bleu. If you were going to a university you would be." ok I can get it to you.
I was able to fax both forms thankfully, so another trip down to Houston wasn't required. Fingerprints and a headshot were.
I emailed my landlady to ask about the letter and she offered right away but said three Dutch people had rented the apartment and it was not available during that time. dilemma! I went ahead and faxed my lease over without the letter to see what would happen. My landlady emailed again, saying she wouldn't mind writing it to say if it were free we could extend the lease. I said sure! why not! best to have a back up. She got it to me within a day! I sent it over to the consulate and gave them a call- my visa was already in the mail! Ends up they didn't look too closely at the lease. haha Hurrahhhhh.
Make sure to hand every form to them. They didn't ask me for the residency form which I'll need when I get to France. They had to stamp it for it to work.
Make a effort to speak a little French.
Ask about the Schnegen region and visiting it (haven't quite worked that out but I did forget to ask when I was there) I think you have to have a special sticker to visit countries outside of it which includes England?
It wasn't too too bad but definitely nerve racking!


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Our servers are positioned in 3 data centers throughout the world - in the United States, in the UK and in Australia. You'll be able to choose the location of your new website hosting account during the signup process, but your website visitors shall not be able to see the difference, since the multi-gigabit connection which we use will guarantee rapid loading speeds for your Internet sites irrespective of the location of the facility that you've picked. The data centers have direct fiber lines to numerous major metropolitan areas in their respective regions and use many different Internet backbone providers to ensure quick and constant access to all of the web servers. Also, we use new powerful hardware for the network that connects the groups on our cloud hosting platform, so as to guarantee fast access to each and every website hosted on it.
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text:
Ann DeGuire
Talent Agency News, Signings, Castings
Broadcast TV News (Shows,News, Promotion)
Auctions, Used Equipment and Software For Sale
Filmmaking Products and Services
Brand Marketers News
Production and Post-Production Products and Services
SIM Group Sponsors "Georgia Night in L.A."
Thursday, Jun. 23, 2016
SIM Group is proud to announce that it is a corporate sponsor of Georgia Night in L.A., a special event developed by the Georgia Film, Music & Digital Entertainment Office to promote film and television production in the Peach State.
Film Commissions, Incentives, Locations News
Graeme Parcher Joins SIM Digital, Los Angeles, as General Manager
Tuesday, Mar. 8, 2016
The SIM Group has appointed former Kodak executive Graeme Parcher as General Manager of the Los Angeles office of its SIM Digital division, a supplier of camera systems and related gear to motion picture and television productions.
SIM Digital Promotes Ann DeGuire to President, Camera Division
The SIM Group, a leading supplier of production and post-production equipment and services to the feature and television industry, today announces the promotion of Ann DeGuire to the newly-created p
Renan Ozturk
Josh Mizrachi
Impossible Brief
Chicago International Film Festival
Patrick Burgoyne


text:
Healthier Perth Amboy
CCCS
PRAHD
RBMC
Faith Based Community
Healthcare Centers
Corner Stores and Resturants
Jewish Renaissance Foundation
149 Kearny Ave #2
www.jrfnj.org
Hours:Monday & Friday: 9am-5pm, Tuesday & Thursday: 12pm-8pm, Wednesday: 10am-6pm
Jewish Renaissance Foundation (JRF) was established in 1995 as a faith-based, nonprofit corporation with central offices in Perth Amboy, New Jersey. Serving more than 2,200 people in Middlesex County each year, they are a leader in providing community coordination, emergency services, education, food and nutrition, family development, and employment and training. The JRF strives to overcome poverty and hopelessness throughout Middlesex County. The JRF helps people in low-income households attain economic self-sufficiency. They provide emergency services to meet immediate needs, while fostering individual growth and transition to household stability. Job training, child care, transportation assistance and other programs are keystones to their mission of promoting greater self-sufficiency.
About Us Work Groups Volunteer Events Contact
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A Thrive Creation


text:
Bilpin businesses open doors to tourists in bushfire recovery operation
Our coverage of the bushfire crisis is free for all readers. Please consider supporting our journalism with a subscription.
By Laura Chung and Nick Moir
What was lush bushland a few weeks ago is today a panorama of blackened trees, as Bilpin business owners play catch up and try to recoup their lost summer income.
On December 19, the Gospers Mountain fire that had been raging for weeks turned towards the town and residents prepared for the worst.
Owner of Hillbilly Cider Shane McLaughlin says people are returning to Bilpin to support local businesses. Credit:Nick Moir
Hillbilly Cider owner Shane McLaughlin had been building a new cellar for people to visit, have a drink and take in the surroundings.
"Now it's nice and calm - it's a black calm," he said.
"I didn't think we had enough fuel for Armageddon, but we did - it was vicious."
The fire on December 21 tore through Bilpin and destroyed properties. Credit:Nick Moir
But now the town's residents are determined to rebuild.
Many of them rely on the summer influx of tourists as their main source of income but the bushfires scared the visitors away.
For Mr McLaughlin, it meant an income drop of 70-80 per cent overall but "100 per cent of income through the cellar door".
He's surprised by how people have already responded to the community's plea for tourists to visit.
"[The amount of people] has been abnormal - it's four times what it should be," he said. "Everyone is coming up, and they're coming up some distance, from Newcastle, the Sutherland shire and Melbourne.
"January is normally a write-off and everyone normally buggers off, but they're coming up here."
Mr McLaughlin said the support had been invaluable, particularly as he's planning to open the doors to his new cellar in about two weeks.
The building has a rich history. In an earlier life, it was a packing shed and then a community dance hall.
Owner of the Bilpin Fruit Bowl Margaret Tadrosse, who lost about 5500 apple trees and 500 fig trees, said the damage to netting, fruit and equipment exceeded $1 million.
"I can't begin to thank people enough for the support we've been getting since the fires," she said.
"On Sunday, the oven could not keep up [with the people]. People were waiting 20 minutes for their apple pies. The oven was pumping them out all day."
Cherry and apple farmer Sean Lonergan, who is also the Bilpin fire captain, said it would take years for the community to recover.
Mr Lonergan has had three fires go through his property, but he said the December blaze was the worst. It destroyed power poles, water and heat pumps, and orchards.
"There's been lots of damage this time, it's all pretty much gone," he said.
Mr Lonergan's challenge will be what he does next. With no apples or cherries, he's looking into other options: including strawberries, tomatoes or even trying his hand at a "bush tucker" line.
"I am not sure what I am going to do," he said. "But I'm going to give it a go."
Laura Chung
Laura is a journalist for The Sydney Morning Herald.
Nick Moir
Nick Moir is Chief Photographer at The Sydney Morning Herald.


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Hanging glass bowl ceiling light shades became popular during the1920s through to the 1950s. They earned the name 'Flycatcher' for obvious reasons.
This 'Classic Art Deco stepped Flycatcher lampshade comes alive when lit, its amber marbling is quintessential 1920s and 1930s.
I have carefully restored these salvaged lights to their former glory and fitted them with new brass components; chains, glass bowl hooks, 3-hook ceiling fitting, wooden pattress, lamp holder and coloured flex. They have been tested for safety.
Each 'Flycatcher' lampshade comes with everything you need to easily convert your existing ceiling fitting. Wiring should be carried out by a qualified electrician.
The Classic Art Deco stepped Flycatcher glass bowl ceiling light with amber marbling comes complete with a 12w LED (Non-Dimmable) bulb.


Interoute Frankfurt Data Center is located at Weismüllerstraße 20, 60314 Frankfurt am Main, Hessen, Germany. The gross building size for this location has not been provided. The gross colocation space is not available for this data center. No power information has been offered for this location. We found 51 data center locations within 50 miles of this facility. Certifications for this location include PCI-DSS, ISAE-3402, ISO 27001, SSAE-16 Type 2.
Interoute - Frankfurt Data Center is located at 20 Weismüllerstraße, Frankfurt am Main 60314, Hessen, Germany}. Sales 877-406-2248. Interoute owns the largest Next Generation Network covering the European Union, from London to Warsaw, from Stockholm to Sicily and beyond into the Continent's emerging economies, including Turkey, with nine subsea landing stations ringing the edge of Europe. To the West, the network links to North America's major telecoms hub. To the East, the network connects the Middle East to Europe through Dubai. In the South, Africa from Cape Town to Tunis connects directly to Europe through Interoute.


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Cowichan Tribes members voted yes on an education jurisdiction agreement and law-making protocol that will return the inherent right to govern education to the community. (File photo)
Cowichan Tribes votes to reclaim the right to educate its children
First Nation one of 13 negotiating with the federal government since 2006
Sarah Simpson
Members of Cowichan Tribes voted on Jan. 8 to reclaim the right to govern how B.C.'s largest First Nation's children are educated.
"This is an exciting first step as we reclaim our inherent right to educate our children," said Stephanie Atleo, the education jurisdiction negotiator for Cowichan Tribes. "Work will now begin on drafting education laws that will outline how we govern education for on-reserve schools. Once these are complete, community will then have an opportunity to review, provide input and approve these laws."
SEE RELATED: Cowichan Tribes' members to vote on education agreement
The historic vote was 15 years in the making, Atleo said, and Cowichan is one of four B.C. First Nations that have accepted the education jurisdiction agreement and law-making protocol, and another First Nation is also holding their vote this week.
Cowichan and 12 other First Nations have been negotiating with the federal government for the right to educate their children since 2006.
"This allows us to create our own curriculum — meaning more culture, land-based learning and Indigenous ways of knowing," said Atleo. "Teaching our children has always been a part of our culture. The education jurisdiction agreement returns a key cultural piece to our community."
Once the agreement has been signed by the Government of Canada, the education responsibilities will fall upon the First Nations for the 2022/2023 school year.
As the First Nation embarks on a new endeavour, they'll have the support of their peers at the Cowichan Valley School district.
"We are happy that Cowichan Tribes has taken this historic step towards reclaiming the right to educate their own children on their own lands. We continue to be proud supporters of Cowichan Tribes, and all nations served by the district, and will continue to work closely with them as the path ahead unfolds," said Candace Spilsbury, chair of the Board of Education for the Cowichan Valley School District.
Cowichan Tribescowichan valleyEducation
Man charged in connection to vandalism of Komagata Maru memorial in Vancouver
U.S. man who re-entered Canada 20 minutes after being deported loses appeal of 9 month jail term


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UIS Events: Students invited to have "Chicken Wings with the Chancellor"
Students invited to have "Chicken Wings with the Chancellor"
WHAT: Students are invited to talk to Chancellor Susan J. Koch about their experiences at UIS during "Chicken Wings with the Chancellor".
WHEN: Tues., Nov. 5, 2013 at 9 p.m.
DETAILS: The forum will allow students to share their ideas and opinions with Chancellor Koch over FREE chicken wings in an informal setting.
This is the fifth forum in a series designed to create an ongoing open exchange of ideas between students and Chancellor Koch. The idea is to make the campus' top leader more available to students in a comfortable environment.
This event is co-sponsored by the Black Male Collegiate Society, Black Student Union, Greek Affairs Board, International Student Association, Organization of Latin American Student, Student Athlete Advisory Committee, and the Student Government Association.
UIS Theatre presents "Clybourne Park"


As a child, Panos enjoyed sketching and working with his hands. He thought he wanted to become an architect when he got older, but along the way he got sidetracked and started building architectural models. You can see about half of his life's work if you click on the link, "See what Panos did before Kantyli", in the title on the front page of the website. Building models gave him an opportunity to learn how to visualize what blueprints described and then had figure out how to make whatever in miniature. He loved the attention to details and surreal life models bring.
Over the years Panos honed in his skills and was able to build many models for clients in San Francisco and Chicago. His work continued throughout the years until a recession forced him to look for a new career. He wanted to use all his work experience, his Hellenic heritage, artistic skill and passion and started making Greek themed products. With his new drive he has been able to design and make quite a few products that bear his name and have started to gain appreciation from Greeks all over the United States.


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text:
Rylstone Project
Township History
Routeways
People of the Parish
Pre-History - A Bronze Age Tree Burial at Scale House Barrow
The Reverend Greenwell excavated the barrow in 1864, and we are grateful to the late Richard Hartland (RH) for some additional information relating to the barrow and similar coffin burials via personal communication).
During the years 1860-69 Captain Blake, steward to the Earl of Burlington, resided at Scale House, Rylstone. The adjoining Scale Hill Farm, on which the barrow stands, would be within his domain. In 1863 the centre of the barrow was dug into by the farm tenant, breaking through the coffin and disturbing part of its contents. The following year, Canon William Greenwell, arrived on the scene to make as good an excavation of the previous year's dig and the techniques of the period allowed.
His description is as follows: 'the mound 30 feet in diameter, 5 feet high, made of clay, shallow trench close to the base completely encircling the mound. Currently its diameter is almost 50 feet with a smooth profile and no vestige of a ditch, so it has been "landscaped". Immediately below the surface of the barrow, at the centre, was a layer of flat stones, c.6' in diameter, carefully arranged. Under these the clay was firmly compacted and rested on a thin stratum of dark-coloured earthy matter which was very full charged with charcoal.'
(At Grisethorpe a tree trunk coffin was excavated in 1834 – a layer of branches lay over the coffin, so might the 'charcoal' be the remains of branches? The Tree Trunk Coffin was at that time displayed in Scarborough Museum.) RH described the Grisethorpe coffin as follows:
'Beneath this again was a layer of finer clay or rather clay which appeared to have undergone a process of tempering, below this finer clay and carefully embedded in it, was an oaken coffin laid upon clay and to some extent supported by a few stones, the whole being placed in a light hollow sunk below the surface of the ground.
The coffin was formed of the trunk of an oak tree split in two and then hollowed out. It was 7 ¼ foot long and 1 foot 11 inches wide; the trunk had been cut off at each end and then partially rounded, but on the outside no attempt at squaring or other workmanship had interfered with the natural surface of the timber. The hollow within was 6 feet 4 inches long and 1 foot wide, roughly hewn out, and still showing the marks of the tool employed; the ends inside were finished off square. It was not possible to make out the nature of the tool which had been employed, but the appearances warranted the conclusion that it had been a narrow-edged metal implement.
The coffin was very much broken in consequence of the disturbance before mentioned; it was however still sufficiently entire to allow its arrangement to be seen. It was laid north and south, having the thicker end – where the head of the enclosed body no doubt had been placed - to the south. The body had entirely gone due to decay and nothing was observed which might have formed a constituent part of it except an unctuous whitish substance, which chemical analysis has proved to be of animal origin (1). The corpse had been enveloped in a woollen fabric, enough of which remained to show that it had reached from head to foot. It was very rotten, and partly on that account, and partly by reason of the infiltration of the earth which had found its way into the coffin through the breakage occurring when the barrow was first opened, and which had become mixed up with the cloth, it was impossible to recover any but small pieces of it, or to prove whether the body had been laid in the grave in its ordinary dress or simply wrapped in a shroud. It is on the whole probable that in this case, as in those of some tree-burials discovered in Denmark the person had been interred in the dress worn by him in daily life, though perhaps it may be alleged that the absence of anything like a button or other fastening is rather against that view.
The material is now of a dark-brown colour, due most likely to the tannin in the of the coffin; whilst to the acid generated in the decaying wood and set free by the percolation of water is perhaps to be attributed the total disappearance of the bones. There was nothing found in the coffin besides the woollen stuff; nor, with the exception of pieces of charcoal and some burnt earth, was anything met with foreign to the ordinary material of the rest of the barrow.
In the absence of any associated articles in the coffin, or of potsherds or flints in the mound itself, it is difficult to assign a precise date or period to this remarkable burial. But if we take the general shape and construction of this barrow into consideration, as also the encircling ditch, the presence of charcoal and other indications of burning, I see no reason for hesitating to refer it to the people whose usual custom it was to place the body of the dead person in a stone cist or in a grave within the barrow; merely supposing that in this and in a few other instances they departed from their ordinary practices in favour of a wooden receptacle.
And when we compare this burial with some others found in this country, and with those which have occurred in Denmark, we can further have little doubt about attributing it to the time when bronze was in use for weapons and implements. The mode of interment in the trunk of as hollowed tree placed within a barrow is no doubt rare, although burials in cleft and hollowed trees placed in the ground without any super incumbent grave-mound are not so uncommon; many of them however are not to be referred to a very early period, and indeed probably belong to a time several centuries after the Christian era.'
In Northumberland, several wooden coffins like this were found near Featherstone Castle, Wyden Eels, in a wet situation; bones entirely decayed except for one instance where bones remained, resembling old leather. The hollows within the bones were filled with the mineral Viviamite. Bones found in peat bogs sometimes are in the same condition, the change in them is due to the action of the carbonic acid generated in the peat.
Radiocarbon dating has recently been done on the Rylstone oak coffin and the woollen material from the Rylstone log-coffin burial (Melton et al. 2016). It has shown that the dates were c. 800 bc. the cusp of the Bronze Age - Iron Age transition. This means that the Rylstone burial is only one of two sites in Britain and is unparalleled in north-western Europe at a time when disposal of the dead was primarily through dispersed cremated or unburnt dis articulated remains. The other coffin burials have been found on radiocarbon dating to be ad 430-970.
Photograph of the woollen clothing from the Craven Museum, Skipton.
Photograph of a piece of timber from the Tree Burial.
Greenwell, W. and Rolleston G. (1877). British Barrows, A Record of the Examination of Sepulchral Mounds in Various Parts of England. Oxford: Clarendon Press.
Grinsell, L.V. (2nd Ed. 1953). The Ancient Burial Mounds of England. London: Methuen & co.
Melton, N.D., Montgomery, J., Roberts, B. W., Cook, G. and Harris, S. (2016). On the Curious Date of the Rylstone Log-Coffin Burial. Available on-line at
https://doi.org/10.1017/ppr.2016.5.
Williamson, W.C. (3rd Ed. 1872). Description of the Tumulus opened at Gristhorpe near Scarborough. Scarborough: S. W. Theakston.
Williamson, W. C. (1896). Reminiscences of a Yorkshire Naturalist. London: George Redway.
Article from Times Past Magazine
(Winter 2005-2006)
Below is a link to the Historical Environment Records' page.


Here's a simple graphic organizer for teaching the life cycle of a bee. Here's the key for the "_____ days" part: egg – 3 days, larva – 6 days, pupa – 12 days. In the boxes, kids can write notes, draw pictures, etc.
This entry was posted in Graphic Organizers, Social Studies and tagged Bee, Children, Common core, Education, Elementary School, graphic organizers, Homeschool, Kids, Life cycle, Science, Teacher Resources, Teachers, Teaching by Squarehead Teachers. Bookmark the permalink.