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Author: Li Wang (61 Results)
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You are looking at 1 - 10 of 61 items for
Author: Li Wang x
Page:123456
Role of microRNAs in mammalian spermatogenesis and testicular germ cell tumors
Li Wang and Chen Xu
microRNAs (miRNAs) are a class of small endogenous RNAs, 19–25 nucleotides in size, which play a role in the regulation of gene expression at transcriptional and post-transcriptional levels. Spermatogenesis is a complex process through which spermatogonial stem cells (SSCs) proliferate and differentiate into mature spermatozoa. A large number of miRNAs are abundantly expressed in spermatogenic cells. Growing evidence supports the essential role of miRNA regulation in normal spermatogenesis and male fertility and cumulative research has shown that this form of regulation contributes to the etiology of testicular germ cell tumors (TGCTs). In this review, we addressed recent advancements of miRNA expression profiles in testis and focused on the regulatory functions of miRNA in the process of SSC renewal, spermatogonial mitosis, spermatocyte meiosis, spermiogenesis, and the occurrence of TGCTs.
Interference RNA-based silencing of endogenous SMAD4 in porcine granulosa cells resulted in decreased FSH-mediated granulosa cells proliferation and steroidogenesis
Wei Wang, Xia Chen, Xinxiu Li, Li Wang, Haiyan Zhang, Yu He, Jingjing Wang, Yongyan Zhao, Baole Zhang and Yinxue Xu
FSH plays a critical role in granulosa cell (GC) proliferation and steroidogenesis through modulation by factors including bone morphogenetic proteins family, which belongs to transforming growth factor β (TGFB) superfamily. TGFBs are the key factors in maintaining cell growth and differentiation in ovaries. However, the interaction of FSH and TGFB on the GCs' proliferation and steroidogenesis remains to be elucidated. In this study, we have investigated the role of SMAD4, a core molecule mediating the intracellular TGFB/SMAD signal transduction pathway, in FSH-mediated proliferation and steroidogenesis of porcine GCs. In this study, SMAD4 was knocked down using interference RNA in porcine GCs. Our results showed that SMAD4-siRNA causes specific inhibition of SMAD4 mRNA and protein expression after transfection. Knockdown of SMAD4 significantly inhibited FSH-induced porcine GC proliferation and estradiol production and changed the expression of cyclin D2, CDK2, CDK4, CYP19a1, and CYP11a1. Thus, these observations establish an important role of SMAD4 in the regulation of the response of porcine GCs to FSH.
Global gene profiling analysis of mouse uterus during the oestrous cycle
YF Tan, FX Li, YS Piao, XY Sun and YL Wang
Many genes related to the cyclic changes of the uterus during the oestrous cycle have been identified using a one-by-one approach. In the present study, cDNA microarray technology was applied to investigate the global profile of gene expression of mouse uterus at the oestrous and dioestrous stages. At a certain stage of the oestrous cycle, the uteri of mature CD-1 mice (n=10) were removed, pooled and snap-frozen in liquid nitrogen. Total RNA was extracted to synthesize cDNA probes for microarray assay. By screening 8192 mouse genes and expressed sequence tags (ESTs), 51 upregulated and 51 downregulated genes were identified in oestrous uterus, of which 62 are well characterized and 40 are ESTs. The known genes were assigned to various gene categories according to their main function. The microarray was performed three times with three independent sets of uterine tissue pools. The results of northern blot analysis for small proline-rich protein 2 (Sprr2), 17beta-hydroxysteroid dehydrogenase type 2 (17betaHSD-2), high mobility group 2 (Hmg2), mitotic checkpoint component 2 (Mad2) and an EST AW555366 mRNA were consistent with that of microarray analysis. In situ hybridization was performed to localize the transcript of the EST AW555366. Most of the upregulated genes encode secreted immune-related proteins, proteinases and their inhibitors, indicating their potential involvement in sperm viability as well as capacitation. The downregulated genes mainly encode cell cycle-related factors, implying the active proliferation of uterus at dioestrus.
MiRNA-335-5p negatively regulates granulosa cell proliferation via SGK3 in PCOS
Lihua Yao, Mingyang Li, Jingwen Hu, Wangsheng Wang and Minzhi Gao
Polycystic ovary syndrome (PCOS) is a major cause of infertility in women of reproductive age. However, its exact etiology remains unknown. In this study, we sequenced miRNAs in human follicular fluid and identified 16 downregulated and 3 upregulated miRNAs in PCOS group compared with non-PCOS group. Among the differential expressed miRNAs, miR-335-5p was verified lower abundance in PCOS than non-PCOS group using quantitative real-time PCR. Besides, miR-335-5p negatively correlated with antral follicle count, anti-Müllerian hormone and total testosterone. Bioinformatics analysis identified serum/glucocorticoid-regulated kinase family member 3 (SGK3) as a potential target gene of miR-335-5p. SGK3 is involved in protein kinase B-mammalian target of rapamycin kinase (AKT-mTOR) signaling pathway and cell proliferation. Western blotting and cell counting kit-8 assays demonstrated that miR-335-5p mimic reduced, while miR-335-5p inhibitor increased, SGK3 abundance, AKT-mTOR pathway and cell proliferation in human granulosa-like tumor KGN cells. Dual-luciferase reporter assays showed that miR-335-5p binds to the 3′ untranslated region of SGK3 mRNA. Furthermore, miR-335-5p was decreased and SGK3 was elevated in human granulosa cells obtained from PCOS patients as compared with non-PCOS controls. These findings suggested that miR-335-5p is involved in granulosa cells proliferation by reducing SGK3 expression, which might provide a molecular target to improve dysfunctional granulosa cells in patients with PCOS.
SMAD7 antagonizes key TGFβ superfamily signaling in mouse granulosa cells in vitro
Yang Gao, Haixia Wen, Chao Wang and Qinglei Li
Transforming growth factor β (TGFβ) superfamily signaling is essential for female reproduction. Dysregulation of the TGFβ signaling pathway can cause reproductive diseases. SMA and MAD (mothers against decapentaplegic) (SMAD) proteins are downstream signaling transducers of the TGFβ superfamily. SMAD7 is an inhibitory SMAD that regulates TGFβ signaling in vitro. However, the function of SMAD7 in the ovary remains poorly defined. To determine the signaling preference and potential role of SMAD7 in the ovary, we herein examined the expression, regulation, and function of SMAD7 in mouse granulosa cells. We showed that SMAD7 was expressed in granulosa cells and subject to regulation by intraovarian growth factors from the TGFβ superfamily. TGFB1 (TGFβ1), bone morphogenetic protein 4, and oocyte-derived growth differentiation factor 9 (GDF9) were capable of inducing Smad7 expression, suggesting a modulatory role of SMAD7 in a negative feedback loop. Using a small interfering RNA approach, we further demonstrated that SMAD7 was a negative regulator of TGFB1. Moreover, we revealed a link between SMAD7 and GDF9-mediated oocyte paracrine signaling, an essential component of oocyte–granulosa cell communication and folliculogenesis. Collectively, our results suggest that SMAD7 may function during follicular development via preferentially antagonizing and/or fine-tuning essential TGFβ superfamily signaling, which is involved in the regulation of oocyte–somatic cell interaction and granulosa cell function.
Delayed implantation in giant pandas: the first comprehensive empirical evidence
Hemin Zhang, Desheng Li, Chendong Wang and Vanessa Hull
Successful conservation of an endangered species relies on a good understanding of its reproductive biology, but there are large knowledge gaps. For example, many questions remain unanswered with regard to gestation and fetal development in the giant panda. We take advantage of a sample size that is unprecedented for this species (n=13) to explore patterns in reproductive development across individuals at the China Conservation and Research Center for the Giant Panda. We use ultrasound techniques on multiple giant pandas for the first time to empirically confirm what has long been suspected that pandas exhibit delayed implantation of the embryo. We also show that the duration of postfetal detection period is remarkably similar across individuals (16.85±1.34 days). Detection of fetus by ultrasound was strongly correlated to the peak in urinary progesterone (r=0.96, t=8.48, d.f.=8, P=0.0001) and swelling in the mammary glands (r=0.79, t=3.61, d.f.=8, P=0.007) and vulva (r=0.91, t=6.40, d.f.=8, P=0.0002) of adult females. When controlling for both the duration of the total gestation period and the postfetal detection period, infant birth weight was only significantly predicted by the latter (β=11.25, s.e.m.=4.98, t=2.26, P=0.05), suggesting that delayed implantation increases flexibility in the timing of birth but is not important in dictating infant growth. This study informs reproductive biology by exploring the little-studied phenomenon of delayed implantation in relationship to physiological changes in pregnant giant panda females.
Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes
Dong Han, Xin-Yan Cao, Hui-Li Wang, Jing-Jing Li, Yan-Bo Wang and Jing-He Tan
Although studies suggest that the low competence of oocytes from prepubertal animals is due to their insufficient cytoplasmic maturation and that FSH improves oocyte maturation possibly by retarding meiotic progression and allowing more time for cytoplasmic maturation, the mechanisms by which puberty and gonadotropins regulate meiotic progression require additional detailed studies. For the first time, we observed that while meiotic progression was significantly slower, the maturation-promoting factor (MPF) activity of oocytes was significantly higher in prepubertal than in adult mice. To resolve this contradiction, we specified the molecules regulating the MPF activity and their localization during oocyte maturation in prepubertal and adult mice primed with or without gonadotropins. Our tests using corresponding enzyme regulators suggested that while activities of protein kinase A were unaffected, the activity of adenylate cyclase (ADCY) and phosphodiesterase increased while cell division cycle 2 homolog A (CDC2A) decreased significantly after puberty. While most of the adult oocytes had CDC2A protein concentrated in the germinal vesicle (GV) region, the majority of prepubertal oocytes showed no nuclear concentration of CDC2A. Maximally priming mice with equine chorionic gonadotropin brought the above parameters of prepubertal oocytes close to those in adult oocytes. Together, the results suggest that puberty and gonadotropin control oocyte meiotic progression mainly by regulating the ADCY activity and the concentration of the activated MPF toward the GV region.
Prevention of embryo loss in non-obese diabetic mice using adoptive ITGA2+ISG20+ natural killer-cell transfer
Yi Lin, Huiqi Wang, Wenjing Wang, Shan Zeng, Yanmin Zhong and Da-Jin Li
Both regulatory T cells and regulatory natural killer (NK) cells may play essential roles in the maintenance of pregnancy. In this study, we show that a significantly high percentage of spontaneous embryo loss was observed in both allogeneic and syngeneic pregnant non-obese diabetic (NOD) mice. The percentage of embryo loss in allogeneic pregnant mice was further increased by the administration of anti-asialo ganglio-N-tetraosylceramide to deplete NK cells, but was decreased by the adoptive transfer of ITGA2+ISG20+ (CD49b+ CD25+) NK cells from normal mice. No such trend was observed in syngeneic pregnant NOD mice. The pattern of CXCR4 (specific receptor for CXCL12) expression on NK cells was analyzed and NK-cell migration was confirmed by in vitro and in vivo migratory assays. Since CXCL12 production by murine trophoblast cells was confirmed previously, our findings suggest that the recruitment of peripheral CXCR4-expressing ITGA2+ISG20+ NK cells into pregnant uteri may be important in the regulation of feto-maternal tolerance.
Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion
Kun Li, Yue Liu, Xiaoyu Xia, Li Wang, Meige Lu, Yanqin Hu and Chen Xu
Bactericidal/permeability-increasing protein (BPI) is a 455-residue (∼55 kDa) protein found mainly in the primary (azurophilic) granules of human neutrophils. BPI is an endogenous antibiotic protein that belongs to the family of mammalian lipopolysaccharide (LPS)-binding and lipid transport proteins. Its major function is to kill Gram-negative bacteria, thereby protecting the host from infection. In addition, BPI can inhibit angiogenesis, suppress LPS-mediated platelet activation, increase DNA synthesis, and activate ERK/Akt signaling. In this study, we found that Bpi was expressed in the testis and epididymis but not in the seminal vesicles, prostate, and solidification glands. BPI expression in the epididymis increased upon upregulation of testosterone, caused by injection of GNRH. In orchidectomized mice, BPI expression was significantly reduced, but its expression was restored to 30% of control levels in orchidectomized mice that received supplementary testosterone. The number of sperm fused per egg significantly decreased after incubation with anti-BPI antiserum. These results suggest that BPI may take part in the process of sperm–oocyte fusion and play a unique and significant role in reproduction.
Dual effect of transforming growth factor β1 on cell adhesion and invasion in human placenta trophoblast cells
Mei-rong Zhao, Wei Qiu, Yu-xia Li, Zhi-bin Zhang, Dong Li and Yan-ling Wang
Transforming growth factor β (TGFβ) has been shown to be a multifunctional cytokine required for embryonic development and regulation of trophoblast cell behaviors. In the present study, a non-transformed cell-line representative of normal human trophoblast (NPC) was used to examine the effect of TGFβ1 on trophoblast cell adhesion and invasion. In vitro assay showed that TGFβ1 could significantly promote intercellular adhesion, while inhibiting cell invasion across the collagen I-coated filter. Reverse transcription (RT)-PCR and gelatin zymography demonstrated that TGFβ1 evidently repressed the mRNA expression and proenzyme production of matrix metalloproteinase (MMP)-9, but exerted no effect on mRNA expression and secretion of MMP-2. On the other hand, both the mRNA and protein expression of epithelial-cadherin and β-catenin were obviously upregulated by TGFβ1 in dose-dependent fashion, as revealed by RT-PCR and western-blot analysis. What is more, one of the critical TGFβ signaling molecules – Smad2 was notably phosphorylated in TGFβ1-treated NPC cells. The data indicates that cell invasion and adhesion are coordinated processes in human trophoblasts and that there exists paracrine regulation on adhesion molecules and invasion-associated enzymes in human placenta.
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A prospective, randomized, controlled trial of benzodiazepines and nitroglycerine or nitroglycerine alone in the treatment of cocaine-associated acute coronary syndromes
Am J Emerg Med. 2003 Jan;21(1):39-42. doi: 10.1053/ajem.2003.50010.
Tim Honderick 1 , David Williams, David Seaberg, Robert Wears
1 Department of Emergency Medicine, University of Florida Health Science Center, Jacksonville, FL 32610, USA.
DOI: 10.1053/ajem.2003.50010
The purpose of the present study was to compare the use of lorazepam plus nitroglycerine (NTG) versus NTG alone in the reduction of cocaine induced chest pain in the emergency department. The secondary objective of the study was to help determine the safety of lorazepam in the treatment of cocaine- associated chest pain. The study was a prospective, randomized, single-blinded, controlled trial conducted at an university-affiliated urban emergency department (ED). All patients who presented with cocaine-associated chest pain were enrolled. Exclusion criteria included age greater than 45 years, documented coronary artery disease, chest pain of more than 72 hours duration, or pretreatment with nitroglycerin. Patients were given either sublingual nitroglycerine (SL NTG) (Group 1) or SL NTG plus 1 mg of lorazepam intravenously (Group 2) every 5 minutes for a total of 2 doses. Chest pain was recorded on an ordinal scale of 0 to 10 at baseline, and then at 5 minutes after each dose. Adverse reactions to medication were also recorded. Twenty-seven patients met the inclusion criteria and were enrolled in the study. The average age of these subjects was 34.1 years, and 67% were men. The NTG-only group consisted of 15 patients and the NTG-plus-lorazepam group consisted of 12 patients. Baseline mean chest-pain scores were 6.87 in Group 1 and 6.54 in Group 2, with no differences between groups. Five minutes after initial treatment, mean scores for the two groups were 5.2 and 3.9, respectively, with a difference in means of 1.24 (95% confidence interval [CI] -0.8-3.8). Five minutes after the second treatment, the mean scores were 4.6 and 1.5, respectively, with a difference in means of 3.1 (95% CI 1.2-5). Kruskal-Wallis testing showed a significant difference in pain relief between the two study groups (P =.003), with greater pain relief noted at 5 and 10 minutes in the NTG-plus-lorazepam group (P =.02 and P =.005, respectively). All patients in the study were admitted to the hospital, but no patient in either group had an acute myocardial infarction or cardiac complications in the ED. No adverse side effects were noted for either group. The early use of lorazepam with NTG was more efficacious than NTG alone, and appears to be safe in relieving cocaine-associated chest pain.
Copyright 2003, Elsevier Science (USA). All rights reserved.)
Acute Disease
Angina Pectoris / chemically induced*
Angina Pectoris / drug therapy*
Anti-Anxiety Agents / administration & dosage*
Anti-Anxiety Agents / adverse effects
Anti-Anxiety Agents / therapeutic use*
Cocaine / adverse effects*
Lorazepam / administration & dosage*
Lorazepam / adverse effects
Lorazepam / therapeutic use*
Nitroglycerin / administration & dosage*
Nitroglycerin / adverse effects
Nitroglycerin / therapeutic use*
Single-Blind Method
Vasoconstrictor Agents / adverse effects*
Anti-Anxiety Agents
Vasoconstrictor Agents

redpajama_set_name: RedPajamaCommonCrawl


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info@johnogroatsguesthouse.com
John o' Groats Guest House
John o' Groats
Caithness. KW1 4YR
The Snug
Evening Meals
Natural Caithness
Congratulations to Mark Moore!
Our guest Mark Moore set off from John o' Groats on 7th April and arrived at Land's End on 15th April, having completed the cycle ride in 8 and a half days. Mark is raising money for The British Heart Foundation and Breast Cancer Research.
This is Mark's second 'End-to-end' after completing this challenge 21 years ago. We send him our congratulations.
lands end to john o' groats
Right place, right time...
Up and Running - 2018
Flower of Scotland...
Thumbs up for Doug!
Snow Joke!
The Mighty Quinn!
JogLe at 82!!!
LeJog in a vehicle you've built yourself!
Lands End to John o' Groats on a Penny-farthing.
Lands End to John o' Groats on a bike and the London Marathon just 2 weeks later!
JogLe
© 2022 John o' Groats Guest House


April 2014 - Client complaints - an inevitability of heightened expectations or a route to improved service?
Client complaints - an inevitability of heightened expectations or a route to improved service?
Previously we looked at expressions of dissatisfaction.
By way of illustration, a scenario from an alternative consumer service experience.
A car owner decides that it is time their pride and joy has the love and attention of a main dealer MOT, full service and all round check up. The experience as a customer is excellent, with a full explanation of all that will be done and, on collection, all that has been done, including discussion with the mechanic and a view of the car on the ramps to see the work.
The payment of £800 for the pleasure of being looked after by one of the top service centres in the city was palatable, despite the shock of the hourly rate of £120 plus Vat.
What rather took the gloss off the service was on driving away, discovering that the new brake fluid had not been bled through, leading to virtually zero braking ability. Followed by a swift about turn to the main dealer and a robust discussion.
This scenario is just by way of example that it can be a small error that takes the shine off an otherwise exemplary service experience and leads to a less than happy memory, even if there was no loss or claim to be made akin to a negligence scenario.
The reality check for many lawyers is that acting for clients as individuals, occasionally clients will be unhappy with some aspect of the service they receive.
The key word we look at here is service. This is distinct from an allegation of professional negligence or breach of contract. Indeed it is distinct from an allegation of misconduct. However it is inevitable that those areas may well also raise the cloud of disappointment as to service and to that extent overlap.
As more law firms move towards a culture of consumer service standards ( indeed many law firms have operated in that way for years, whether via Service Level Agreements or Client Relationship Management, or simply by being fundamentally alive to the most important person namely the client), aside from accepting that complaints will happen, other changes are needed.
The utopia is one of no complaints. In that world everyone is delighted with your service and recommends your firm to friends, family and business colleagues in a heartbeat.
Be alive to the role of the LeO if the complaint is brought to their attention.
This way you minimise the potential for damaged reputation, you retain your client for future work for them and you avoid the drain on your time and resource by proper investigation and resolution of the complaint.
Your complaint scheme will have been set out by you in the retainer agreement you entered in to with your client, signed by them and by you. It is that document that will set the parameters, albeit increasingly clients can take another look at your website and publications for what you promise to deliver by way of service levels.
Working backwards, the hope is that the LeO need not be troubled by your clients, thus reducing the workloads on the LeO (and the cost of that operation borne ultimately by the profession - and indeed by the firms suffering the complaints to a point).
The LeO makes very sensible suggestions for what you could have as the basis for your complaints service. This incorporates the fundamentals mentioned earlier. Keeping it simple is attractive, especially as you should resolve the complaint within 8 weeks. If not, then the LeO will accept it for investigating.
You will also have had to be mindful of your contractual obligations to your Professional Indemnity Insurers as to whether the complaint is a claim or a circumstance or an intimation of an intention to make a claim against you. This may also apply to the situation in which you are making an admission of an error or omission and an offer of redress and apology.
This was perhaps put in sharp focus in recent weeks by the news that the LeO is to publish the total number of complaints processed against named law firms. To date the LeO has provided what many consider to be a constructive summary of complaints and their resolution - hopefully going some way ( if read ) to enabling law firms to improve their service.
The LeO website has a consumer focused section on "stories "with snappy titles. They may not all appeal to the lawyers or be particularly detailed as to cause and effect however. Fairly, it seems to take a balanced view, with a number of stories of complaints not upheld at all.
The recent angst was whether publishing in July 2012 a league table of law firms that the LeO have had to become involved with is naming and shaming. Whilst that is a debate that will no doubt rumble on, it is a fact that we live in times of league tables and scores of many aspects of service and performance, be it schools, universities, hotels and restaurants.
As lawyers, firms are entering that consumer driven world with some alacrity. Apparently however of the 75,000 complaints in the first year of the LeO service, only around 7000 needed investigation.
We anticipate it is impossible for any law firm dealing day in day out with clients both new and existing, to have zero complaints and indeed zero claims. It is a case of active risk management in a way that is proportionate to your particular business, to minimise the disappointed clients and maximise the service experience enjoyed both by them and by you in providing it.
Whilst the high service standards of the profession have been and should always be a given, management of them can lead to more widely applicable improved service levels .
This article is a general guide and is not a substitute for professional advice. No responsibility can be taken for any loss incurred by anyone acting or failing to act on the basis of this article.


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Home / Club Rugby / Sale Sharks to partner with troubled Yorkshire Carnegie?
Sale Sharks to partner with troubled Yorkshire Carnegie?
Posted on 4th July 2019 by admin in Club Rugby, Latest News, The Greene King IPA Championship
RIVAL clubs believe that Yorkshire Carnegie could still be relegated from the Championship even though their members have agreed a CVA.
Last Friday, Carnegie released a statement to say that their CVA had been approved by a majority of 100 per cent.
Creditors were offered 15 pence in the pound but for players who've already signed new deals elsewhere that figure only applied to the difference in the value of the two contracts.
Around £1m in player wages will all be settled for a fraction of the sum, rumoured to be as little as £60,000.
But as Carnegie celebrated and a strategic arrangement with Sale Sharks was being discussed, supporters of Richmond asked if Carnegie still had a case to answer for their insolvency. Under RFU rules, a club can be deducted up to 28 points for poor financial management and such a sanction could reprieve Richmond who were relegated to National League One.
Carnegie's statement read: "We will be building a new Yorkshire Carnegie squad that will be competing in the Greene King IPA Championship for the 2019-20 season. New arrangements will be agreed with a new playing squad, staff, sponsors and service providers."
Those arrangements will need to be ratified by the RFU who have to be satisfied under duty-of-care to players that Carnegie would be able to put out a competitive team for early season games.
An RFU spokesperson said: "The RFU is in regular contact with Yorkshire Carnegie given the current financial position of the club. Discussions are ongoing as to the club's plans for next season and the funding it will have available."
Meanwhile Sale Sharks director of rugby Steve Diamond and operations manager Paul Smith met with Carnegie officials last week to discuss ideas that might be of mutual benefit.
Diamond is keen to grow the Sharks brand across the North and one of the plans is to hold a select number of games at the recently renovated Headingley Stadium, Carnegie's home ground.
Diamond is also keen to have an interchangeable player pathway between the clubs which would give academy players on both sides of the Pennines much-needed competitive game time.
Carnegie would be able to tap into a Sale Sharks player and coaching pool as they look to hastily assemble a squad and coaching team from scratch in time for the start of the 2019/2020 season, away to Nottingham in the Championship Cup on the weekend of September 20/21/22.
Carnegie have beaten the odds before in that respect, with Stuart Lancaster inheriting a squad of only seven players when he took charge in 2006 but the current situation is even more drastic. Not only do Carnegie not have any players, they are without an entire coaching/backroom team.
DoR Chris Stirling and head coach Steve Boden have returned to their former clubs, Cornish Pirates and Doncaster, respectively, and strength and conditioning and physio staff also need to be appointed. Meanwhile, academy coaches Richard Beck (Wasps) and Scott Barrow (Sedgley Park) have landed new jobs.
Oxford University's long-serving head coach James Wades, formerly of Sale and Wasps, has been mentioned as frontrunner for the head coach role.
But there are concerns for Carnegie's famed academy. It is hard to see how the club can afford to satisfy RFU rules and put in £250,000 of their own money to help fund the academy.
JON NEWCOMBE
Tagged Sale Sharks, Yorkshire Carnegie
Davies is the right man to salvage Yorkshire Carnegie, says Stuart Lancaster
My Life in Rugby: Former Wasps, Bath and Yorkshire Carnegie prop Charlie Beech
Dream Team: Sale Sharks and ex-Stade Francais back row Jono Ross


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Igor Sokolov
Zurich Research Laboratory, Zurich, Switzerland
Igor Sokolov is a PhD student at IBM Research - Zurich team working on the development of new quantum algorithms for optimisation and quantum chemistry simulations.
Igor received his BSc in Physics from the Swiss Federal Institute of Technology in Lausanne (EPFL). He continued his studies at the Swiss Federal Institute of Technology in Zurich (ETHZ) to obtain his MSc in Theoretical Physics. He wrote his Master's thesis on "Quantum algorithms for electronic structure calculations" supervised by Prof. Manfred Sigrist at ETHZ and Dr. Ivano Tavernelli at IBM. His work contributed to the publication "Quantum algorithms for electronic structure calculations: particle/hole Hamiltonian and optimized wavefunction expansions" by Panagiotis Kl. Barkoutsos et al.
In his leisure time, Igor enjoys playing piano, composing music, running and playing tennis.


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The 1954 Allan Cup was the Canadian senior ice hockey championship for the 1953–54 senior "A" season.  The event was hosted by the Penticton V's and Penticton, Vernon, and Kelowna, British Columbia.  The 1954 playoff marked the 46th time that the Allan Cup has been awarded.

Teams
Sudbury Wolves (Eastern Canadian Champions)
Penticton V's (Western Canadian Champions)

Playdowns

Allan Cup Best-of-Seven Series
Sudbury Wolves 2 - Penticton Vees 1
Penticton Vees 6 - Sudbury Wolves 2
Sudbury Wolves 3 - Penticton Vees 1
Sudbury Wolves 8 - Penticton Vees 2
Penticton Vees 6 - Sudbury Wolves 5 (OT)
Penticton Vees 6 - Sudbury Wolves 4
Penticton Vees 3 - Sudbury Wolves 2

Eastern Playdowns
Semi-final
Matane Red Rocks defeated Fredericton Capitals 4-games-to-none
Matane Red Rocks 4 - Fredericton Capitals 0
Matane Red Rocks 8 - Fredericton Capitals 3
Matane Red Rocks beat Fredericton Capitals
Matane Red Rocks 3 - Fredericton Capitals 2
Sudbury Wolves defeated Owen Sound Mercurys 4-games-to-3
Sudbury Wolves 4 - Owen Sound Mercurys 1
Sudbury Wolves 4 - Owen Sound Mercurys 0
Owen Sound Mercurys 5 - Sudbury Wolves 2
Owen Sound Mercurys 3 - Sudbury Wolves 1
Owen Sound Mercurys beat Sudbury Wolves
Sudbury Wolves 3 - Owen Sound Mercurys 1
Sudbury Wolves 4 - Owen Sound Mercurys 1
Final
Sudbury Wolves defeated Matane Red Rocks 4-games-to-2 with 1 tie
Sudbury Wolves 4 - Matane Red Rocks 4
Matane Red Rocks 3 - Sudbury Wolves 2
Sudbury Wolves 5 - Matane Red Rocks 3
Sudbury Wolves 4 - Matane Red Rocks 2
Sudbury Wolves 5 - Matane Red Rocks 3
Matane Red Rocks 5 - Sudbury Wolves 1
Sudbury Wolves 3 - Matane Red Rocks 0

Western Playdowns
Quarter-final
Winnipeg Maroons defeated Fort William Beavers 4-games-to-none
Winnipeg Maroons 2 - Fort William Beavers 1
Winnipeg Maroons 1 - Fort William Beavers 0
Winnipeg Maroons 3 - Fort William Beavers 1
Winnipeg Maroons 6 - Fort William Beavers 1
Semi-final
Winnipeg Maroons defeated Moose Jaw Millers 4-games-to-2 with 1 tie
Winnipeg Maroons 4 - Moose Jaw Millers 1
Moose Jaw Millers 3 - Winnipeg Maroons 2
Winnipeg Maroons 7 - Moose Jaw Millers 6
Winnipeg Maroons 3 - Moose Jaw Millers 3
Moose Jaw Millers 3 - Winnipeg Maroons 2
Winnipeg Maroons 8 - Moose Jaw Millers 2
Winnipeg Maroons 9 - Moose Jaw Millers 1
Final
Penticton Vees defeated Winnipeg Maroons 3-games-to-2 with 3 ties
Penticton Vees 4 - Winnipeg Maroons 4
Winnipeg Maroons 3 - Penticton Vees 2
Penticton Vees 7 - Winnipeg Maroons 1
Penticton Vees 4 - Winnipeg Maroons 4
Winnipeg Maroons 4 - Penticton Vees 0
Penticton Vees 4 - Winnipeg Maroons 4
Penticton Vees 5 - Winnipeg Maroons 1
Penticton Vees 5 - Winnipeg Maroons 2

References
Allan Cup archives

External links
Allan Cup website

1953–54 in Canadian ice hockey
Allan Cup

redpajama_set_name: RedPajamaWikipedia


Although rewarding and exhilarating the life and work of a barrister and clerk is too often demanding and pressured. When pressure becomes chronic you may experience some of the stress response symptoms outlined in this short film. These are early warning signs, informing you that you need to start making healthy choices. Dr Bill Mitchell presents an animation explaining wellbeing and what you can do to maintain your resilience and perform well.
Wellbeing is defined as being as physically fit as you can be, enjoying life and work, being connected to positive others and retaining an ability to both keep perspective about and ability to recover from difficult times.
A significant piece of work by The New Economics Foundation identified five drivers of personal wellbeing. These are strongly supported by Mind, the mental health charity and the NHS.
Have you noticed that life moves faster these days?
After years of research, experts on stress and depression like Professor Mark Williams at Oxford University have concluded that our lives are indeed more full and demands are higher on all of us. It can sometimes feel that life is moving at an unrelenting pace. Consider how frequently you have a conversation and struggle to recall the details afterwards, or travel to court or chambers and barely remember a moment of your journey. Taking a moment to pause often seems like a luxury we cannot afford.
Compelling evidence has been building in the past forty years which unequivocally demonstrates the value in becoming more aware, moment to moment. When we are able to live more in the present we start to train our brains to reduce rumination and worrying about future states. This leads to increased positive mental states, self-regulated behaviour and boosts self-knowledge and awareness.
If ever there was a 'silver bullet' for great all round personal wellbeing, it is regular physical activity.
Regular physical activity also has known mental health benefits. The risk of depression is lowered by 30%. Cognitive decline is also reduced by up to 30%. Physical activity has a powerful impact on the increased production of endorphins (those 'feel good' hormones) and a reduction in cortisol levels (the 'stress' hormone).
Studies demonstrate that physical activity also benefits concentration and focus; Dr John Ratey, a Harvard Professor of Psychiatry, recommends taking physical activity before an important public performance in order to sharpen focus.
Memory and mood are boosted by being more active, as is productivity. These are all hugely important skills for your successful career. Being able to regulate emotional responses, remember key case law and use your time efficiently both in and out of the courtroom gives you a huge advantage over the opposition.
It may be tempting to treat physical activity in the same way as our busy lives and work out intensely. However, on a stressed body and mind this can have a negative impact. If you are experiencing symptoms of extreme fatigue, a hard bout of physical activity will put the body under too much strain. At times like these, choose gentle stretching or walking instead and respect the repair and recovery that your body requires.
Consider your life as a barrister. You may have frequent long commutes to court and find that being able to get involved in social interaction with colleagues is difficult because you don't live locally. Long working hours are inevitable in our profession, reducing time available with family and friends. Perhaps you live in the city for part of the week and return home at weekends. Variable working patterns and working long into the night preparing for court, make it hard to commit to social engagements.
Human beings are social creatures and we instinctively know that we need good quality relationships to lead a fulfilled life. Studies on the over 50's from the University of Chicago demonstrated that loneliness can be twice as unhealthy for us as obesity. Younger people (18 -34 years) are experiencing loneliness more than ever and this is more likely to result in depression than in older subjects. The Mental Health Foundation found that 42% of us have felt depressed as a result of loneliness.
Scientists know that oxytocin, the hormone responsible for affiliation protects us from cardiac ill-health. This hormone is released when we have a physical presence with people we care about, which means that we cannot solely rely on catching up with family and loved ones via social media and emails.
Being curious and continually learning throughout life have also been shown to drive personal wellbeing measures including life satisfaction scores, optimism and efficacy.
Learning has many benefits including raising self-esteem, confidence and building a sense of purpose. Professor Paul Dolan, a behavioural scientist at the London School of Economics has found that happiness is driven by having a sense of meaning or purpose in our lives, balanced with enjoyment of one off, hedonistic pursuits.
Curiosity is a phenomenon that we see in children but outside our job roles, we may find that as adults our lives have become more one dimensional. Positive psychology (the scientific study of what makes us flourish and thrive) demonstrates that being curious broadens our minds to more possibility in life. This has a direct correlation on our ability to cultivate more positive emotion.
Instinctively it feels good to give to others. We now know that reciprocity also drives long term self-reported wellbeing. Professor Sonia Lyubomirsky of the University of California demonstrated that carrying out one simple act of kindness per week over a six-week period increased wellbeing in participants when compared to a group that committed no acts of kindness.
Giving may look like a formal volunteering exercise or perhaps pro-bono legal opportunity or it could be subtler that this. Small actions can have just as deep an impact on wellbeing as the more obvious ones.
Asking for help is never easy. The world of The Bar is a fiercely independent and proud one. Expectations of quality and service are high. However, you'll provide the best service to your clients when you are at your healthiest and when life and work are in balance. If you feel the balance slipping and stress becoming a normal part of your life, you can take action to bring things back on course.
Knowing your own warning signs allows you to deploy strategies which can help manage your psychological wellbeing in the short and long term. In doing so, you will increase your resilience and greatly reduce the chance of becoming unwell.
Read some of the personal stories offered by barristers who have suffered with mental health issues.
There are a range of organisations which can help with specific issues. Click here for some advice on seeking help and for a list of organisations and their contact details.


text:
A Milestone of Capital Proportion
Mike KnubleWashington Capitals
Knuble celebrates his 1,000th NHL game; veteran is focused on continued success
Mike Knublesees his hockey career as two distinct chapters, with the latter one, which includes playing in his 1,000th NHL game, providing a perfect illustration of his perseverance and professionalism.
It's a milestone he never banked on reaching, but a moment he is certainly grateful to experience.
"It's pretty much all I've talked about it the last little while," Knuble told NHLPA.com, the night before he became the 269th NHLPA player to appear in 1,000 regular season games, in a 4-1 home win against the Nashville Predators. "It's been an interesting road to get to this point. I see it as though I've played two different careers since I came into the league.
"From the time I was 25-30, I was trying to find my way in this league," continued the 39-year-old forward, who has reeled off eight straight seasons of 20 goals. "When I was 30, I had a good year and my career changed direction. It opened doors. Not everyone can be a Hall of Famer, not everyone can be an All-Star, but this there is a place in the game for players who can contribute something."
That would certainly be Knuble, whose teammates honoured him on his big night by wearing his No. 22 jersey during pre-game warm-ups.
Selected by the Detroit Red Wings in the fourth round of the 1991 NHL Entry Draft, Knuble eventually skated into a lineup that was loaded with veteran talent and a team that always seemed to have a big shot at winning the Stanley Cup.
The Toronto-born forward played the next four years at the University of Michigan and was awarded Second Team CCHA All-Star honours in 1994 and 1995 and NCAA West All-American Team honors in 1995. Following his NCAA tenure, he made his professional debut in the 1995 Calder Cup playoffs with the Adirondack Red Wings.
Knuble spent the entire 1995–96 campaign and most of the 1996–97 season with Adirondack before making his NHL debut with the Red Wings on March 26, 1997.
It wasn't necessarily the ideal spot for an eager rookie looking to make his mark, but playing alongside some of the game's top talents would serve him well when he would eventually take on a veteran role.
His name is there to see on the Stanley Cup, when the Wings won it in 1998, and painted on the wall at Joe Louis Arena. Dealt to the Rangers the next season, Knuble tallied 15 goals and 35 points in 82 games in 1998-99, his first opportunity in a full-time role.
With a month left in the 1999–2000 season, the Rangers traded him to the Boston Bruins. After posting 20 points in 82 games in 2000–01 and 14 points in 54 games in 2001–02, Knuble found himself playing left wing on a line with Joe Thornton and Glen Murray beginning in 2002–03. He responded by scoring 30 goals and adding 29 assists in 75 games, putting him third in team scoring.
"That was a huge turning point in my career," recalled Knuble. "I think being able to have that kind of year changed things for me."
After 21 tallies and 25 assists in 82 games the following campaign, he signed with the Philadelphia Flyers in the off-season. After playing in Sweden during the 2004-05 NHL lockout, Knuble had career-best marks in goals (34), assists (31), and points (65) in 2005-06.
"You never really know what your career will be when you first start out," said Knuble, who recorded his first career hat trick on February 2, 2008, scoring all the goals in a 3-0 Flyers win over the Anaheim Ducks. "Early on, you just want to stick. As you go on, you do more things to contribute and there's more of a comfort level. When I was younger, I'd watch players and coaches, seeing how they'd prepare and you'd pick off a few things from them over the years."
Now, it's the younger players on the Capitals, the team he signed with on July 1, 2009, three days before his 37th birthday, who are learning from Knuble, one of the club's alternate captains.
"I can't say there was one bit of advice or one person that I look back on and point to as shaping my career," said Knuble, who has scored 271 career NHL goals, 221 of those coming after he turned 30. "You always strive to be a good teammate, a hard worker and a professional."
As for his big moment, the one where he had plenty of family (his mother came in from Michigan, his brother from Alabama, to name a couple), Knuble likened it to a particular part in the 1983 big-screen comedy Vacation, starring Chevy Chase as a father who leads his family on a cross-country journey from Chicago to a Los Angeles amusement park.
"There's a scene in that movie where they get to the Grand Canyon and they get out of the car, take in the view for a second or two and then jump back in the car and continue on," offered Knuble. "That's what this kind of feels like. It goes by pretty quickly because there are still games left to play and you've got to move on pretty fast to the next thing."
But he knows there will be time, eventually, to savour the moment.
"When you actually have time to sit down and really look at your career, I know it will be even more meaningful," noted Knuble. "When you're still playing, it's tough to look beyond the next game or practice. There is always something going on."
As for the next chapter in his career, No. 22 doesn't quite know what to expect. It's a safe bet Knuble will just do as he has always done. He'll take things game by game, the way he has for 1,000 of them.
My Top 5 | Mike Knuble
Mike Knuble hoisted hockey's most cherished team trophy in 1998 with the Detroit Red Wings. The right-winger, who played 1,068 career NHL regular season games and was a lifetime +76, offers up the most effective ways a forward can make a post-season impact - other than scoring.
Full offseason plate no problem for Hathaway
From fundraising for Hath's Heroes to preparing for his new role as a father, Garnet Hathaway's plate is full and that's just the way he likes it.
The King already feeling at home in the capital
With a visit to his new city and freshly designed gear almost in hand, the Henrik Lundqvist is feeling right at home with the Washington Capitals.
Eller donates Cleanse Portal, encourages support of homeless community
The Eller family has once again stepped up to help others in their community.
Hath's Heroes raises $15K to feed first responders amid COVID-19 pandemic
Garnet Hathaway is playing his part in showing appreciation for first responders during the coronavirus pandemic.
After the Buzzer | Nic Dowd
Hockey player. Father. Husband. Five-star driver. Reporter. Nic Dowd is a little bit of everything.


text:
190715-N-IR096-0031
Seaman Emille Sichz and Seaman Apprentice Ciaran Braswell, students in the Surgical Technician program at the Medical Education and Training Campus at Joint Base San Antonio-Fort Sam Houston perform a mock surgery during a tour. The tour provided San Antonio educators and counselors with a general overview of the Navy in an effort to raise awareness of Navy career opportunities through a tour of the medical training campus.
Petty Officer 1st Class Justin Moore, an instructor with the Hospital Corpsman Basic program at the Medical Education and Training Campus at Joint Base San Antonio-Fort Sam Houston, speaks to a group of San Antonio middle and high school educators during a tour of the campus. The tour provided educators and counselors a general overview of the medical career fields offered in the Navy in an effort to raise awareness of Navy career opportunities.
NEWS | July 25, 2019
Navy Medicine commands host San Antonio educators for STEM tour
By Petty Officer 2nd Class Shayla Hamilton Navy Medicine Training Support Center Public Affairs
JOINT BASE SAN ANTONIO-FORT SAM HOUSTON, Texas –
The Navy Medicine Training Support Center cohosted Bexar County educators for a tour of the Medical Education and Training Campus at Joint Base San Antonio-Fort Sam Houston July 15.
This is the second consecutive year Navy Medicine commands have hosted this event as part of Alamo Science, Technology, Engineering and Mathematics, or STEM, Workforce Coalition efforts, which included a visit to the Naval Medical Research Unit San Antonio, or NAMRU-SA.
Upon arrival, the educators were able to speak with a Navy Recruiting District San Antonio, or NRD-SA, recruiter, who is among the Sailors who service the area of the schools represented. This brief was structured to allow the educators and counselors an opportunity to ask questions about initial-entry requirements, incentives, and education benefits offered to their students upon enlisting. The briefs following allowed the educators to receive insight on the training Sailors, Airmen and Soldiers receive at the Medical Education and Training Campus.
"Here at the METC, we focus a lot of attention on making sure the service members still know how to be a Soldier, Sailor, Airman," said Keith Hall, Strategic Planning and Partnerships Program manager. "We are training them to understand rank structure, training alongside other branches of services helping them to be comfortable when it matters. We train for the mission and educate for a lifetime of service. METC has 49 programs of instruction. On any given day, we see about 5,500 students, which translates to enough people to staff about four 300-bed hospitals."
Hall also informed the educators about the 36 consolidated programs and the credentials students are eligible to receive while training at the METC.
"We work with colleges and universities in the nation to develop degree bridge pathways for our service members," Hall said. "This means they'll allow military students who graduate from the METC to attend their colleges and universities and not have to do the same work that they've already done here. They go into these colleges with a 'head start' already being pre-granted 18-30 credit hours, depending on their program, so they can continue where they left off."
After the briefs, the visitors spoke with Navy instructors from the Radiology and Surgical Technologist programs and observed students performing mock surgeries in the operating rooms.
The last stop on the METC tour was Military Instructional Facility Four, named Anderson Hall, where the educators were presented an overview of the Tactical Combat Casualty Care, or TCCC, portion of the Hospital Corpsman Basic program and toured the spaces where the students put all their training into action during a simulated mass casualty event.
The visit concluded with a panel sat by five Hospital Corpsman Basic instructors. The instructors opened the panel with introductions and gave the guests a brief overview of their Navy careers to better direct their questions.
Medical Education and Training Campus
METC
Navy Science
Engineering and Mathematics
Navy Medicine Training Support Center
Joint Base San Antonio-Fort Sam Houston
Naval Medical Research Unit San Antonio
Navy Recruiting District San Antonio