Weigela plants are shrubs that have beautiful red and pink flowers and can grow to be up to 6 feet (1.8 m) tall. Pruning a weigela plant will help it maintain its shape, promotes healthy growth, and improves flowering. If you follow the proper guidelines when pruning a weigela plant, you can help it to grow healthy and strong!
Saw 2-3 secondary stems to the ground on mature plants. Cutting secondary stems will give you a fuller weigela plant. Locate 2-3 branches that are growing next to the main stem and use a hand-held pruning saw to cut the stems upward at a 45-degree angle. Saw the stems as close to the ground as you can.
This will divert the plant's energy to the main stem and new growth, which will promote stronger blooms.
Cut off the tops of the seed pod branches. Seed pods are left over after the flowers bloom and fall off the plant. They look like buds on the tips of the weigela branches. Use a pair of hand shears to cut the upper growth right above a lower hanging bud on the branch.
Do not cut more than 1/2 of the branches full length.
Make the cut straight, right above a flowering bud.
Cutting away old buds will promote the growth of new, stronger buds which will lead to more robust blooms the next year.
Cut away dead or diseased branches. Look for light grey or brown branches that feel brittle or hollow to the touch. Diseased branches may also have spots or mold on them. Cut these branches down to the main stem, on a 45-degree angle, to make room for new growth and prevent potential disease from spreading.
If the center of the branch is brown or gray, the branch is dead.
Prune off wispy branches. If a branch looks weak or wispy, there's a good chance it will never develop flowering buds. Cut away these branches down to the main stem to promote growth in other parts of the plant.
Leave strong branches that have minimal offshoots and that are capable of growing flowers.
Cut off branches that are rubbing against the main stem. You should cut branches that are rubbing up against the main stem or crossing over, as they can damage the plant. Use a pair of hand shears and cut the branches down to the main stem.
Prune 1/3 of the plant over several seasons to "restart" your plant. Use rejuvenation pruning if you want to start over with your weigela shrub. Prune all the branches down to 1/3 each season until the shrub is 4–10 inches (10–25 cm) in height and there is only new branches left. This is a great way to restart your plant without having to purchase a new one.
You should rejuvenate prune in the late winter.
Prune weigela in the late fall or early spring. Prune your weigela plant after the threat of frost is over, sometime in the late fall or early spring. You can prune the weigela before it blooms to promote more flowers this year or after it blooms to promote stronger growth during the next blooming season.
Weigela should not be pruned after mid-July.
Never prune more than 1/3rd of the plant at a time. Over-pruning a weigela plant can weaken or even kill it. Never prune more than 1/3rd of the entire plant in a single season.
If you need to remove a lot of growth, do it gradually over the course of several seasons.
Prune weigela once a year. Weigela is prone to growing wispy and weak looking branches with a lot of offshoots. This can detract from the natural beauty of the weigela blooms. To prevent these types of branches from taking over your plant, you should prune it once a year.
Use sharp, sterilized pruning shears. Sharp shears will prevent damage to the weigela plant. Wipe down the shears with rubbing alcohol after each cut to prevent the transmission of disease to other plants.
If your shears are breaking or snapping the branches rather than cutting straight through, purchase new ones.
Prune branches above a bud to control the direction of growth. An upward facing bud will continue to grow out and upward, while a downward facing bud will grow downward. Cutting the branch immediately above a bud will allow you to control the direction that it grows in. If you are pruning to maintain the look of your weigela plant, keep this in mind.
When is the best time to prune weigela?
It is best to prune weigela shrubs in the late spring after they have finished blooming.
What do I do if the middle of my weigela looks dead after I pruned it?
Make sure you clean and sanitize your cutting tools using isopropyl alcohol before and after every use.
Invest in a good pair of gardening gloves to wear while you prune. These will help protect your hands and improve your grip.


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text:
Where Do CPAs Work?
CPAs work in public accounting, business and industry, government and education. Within these areas, CPAs generally specialize in what they do.
Listed here are just a few of the professional opportunities the CPA designation can offer you:
Public Accounting — Public accounting is best known for providing audit services. CPAs must be licensed to perform audits. In audits, CPAs review clients' financial statements and voice a professional opinion on them. Within public accounting CPAs also use their skills regarding federal, state and international taxation laws, computer technology and management operations. Staff or entry-level CPAs assist those in charge of an audit engagement. At the manager level, CPAs will supervise in-charge staff. As a partner in a firm, the CPA's responsibilities increase to include supervision of managers and marketing the services of the firm. Partners make decisions that affect every aspect of the firm's operations, and have responsibilities for managing client relationships and obtaining new ones. A CPA who owns his or her own business and may employ a few individuals to support his or her work is known as an individual practitioner.
Industry and Business — Working as a CPA in business and industry requires specialized knowledge for the particular industry. On-the-job training and continuing education is necessary. A CPA may be responsible for developing, producing and reviewing data useful for making business decisions and developing strategic plans. Additionally, CPAs will need to report the data's finding to internal and external parties. CPAs in business and industry have titles including staff accountant, analyst, controller and chief financial officer.
Government — CPAs work in a wide range of positions in local, state and federal government. The federal government employs CPAs in its agencies, including the Internal Revenue Service (IRS), Federal Bureau of Investigation (FBI) and the General Accounting Office (GAO). The IRS audits individuals and corporate tax returns. CPAs working for the FBI are involved in cases including fraud, financial crime and white-collar crime. The GAO examines the use of public funds, evaluates federal programs and activities, and provides information and other assistance to help Congress make effective oversight, policy, and funding decisions. And CPAs working for the State of Florida and its municipal governments perform a wide variance of cost analyses and financial audits. CPAs working in government positions have titles that include FBI or IRS agent, staff accountant, manager, comptroller and director.
Education — Accounting educators are on the faculty of community colleges, universities and graduate schools. CPAs also may hold positions as high school business and accounting teachers. Job titles include accounting instructor, assistant professor, professor and department chair.
More-specialized areas within the accounting profession that are currently popular include the following:
Information Technology — CPAs help many businesses design and implement their computer systems, software applications, and network security.
Consulting — When working as consultants, CPAs may give advice on a variety of issues affecting employees, such as retirement plans, or issues that affect the company, such as purchasing a new building or property.
Forensic Accounting — CPAs in forensic accounting track down fraud, white-collar business crime and insider trading. Through their information gathering and accounting background, CPAs assist in building cases. These CPAs work for law enforcement agencies or in private practice.
Tax and Financial Planning — CPAs help businesses and individuals by recommending savings, investment and tax options. They review financial information and provide advice so prudent decisions can be made. These CPAs also help with international business laws and transactions.

redpajama_set_name: RedPajamaCommonCrawl


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text:
Q: Groupby Month and Year and get the count of unique items as columns I have a Dataframe like the following:
  Date              Desc
 2022-04-31         Car
 2022-05-31         Bus
 2022-05-31         Car
 2022-05-31         Bike
 2022-05-31         Car
 2022-06-14         Car
 2022-06-19         Car
 2022-06-19         Bike
 2022-07-19         Bike
 2022-07-19         Bus

How can I group it by month and year and then get the count of Desc as columns?
Expected Output:
Date     Car  Bus  Bike
2022-04   1    0    0
2022-05   2    1    1
2022-06   2    0    2
2022-07   0    1    1

A: This approach works efficiently, only need to get the dummies and count grouping by the date with the desired frequency:
df = pd.DataFrame({'Desc':['Car','Bus','Car','Bike','Car','Car','Car','Bike','Bike','Bus'],
                   'Date':['2022-04-21','2022-05-31','2022-05-31','2022-05-31','2022-05-31',
                           '2022-06-14','2022-06-19','2022-06-19','2022-07-19','2022-07-19']})
df.Date = pd.to_datetime(df.Date)
df = df.set_index('Date')

pd.get_dummies(df,'','').groupby(pd.Grouper(freq='M')).sum()

result:
Date     Car  Bus  Bike
2022-04   1    0    0
2022-05   2    1    1
2022-06   2    0    2
2022-07   0    1    1

A: You can try pd.crosstab
df['Date'] = pd.to_datetime(df['Date'])

out = pd.crosstab(
    df['Date'].dt.strftime('%Y-%m'), df['Desc']
).reset_index().rename_axis(columns=None)

print(out)

      Date  Bike  Bus  Car
0  2022-04     0    0    1
1  2022-05     1    1    2
2  2022-06     1    0    2
3  2022-07     1    1    0

A: You can also do it manipulating the strings without converting them to datetime. First parse them to year, month and day and put them in a pandas DataFrame
df_ix = pd.DataFrame((x for x in df.Date.str.split("-")), columns=["Y", "M", "D"])

Then concatenate these features with you original DataFrame and use them to group by year, month, and vehicle type. Use count to aggregate the results for each group (keep a single column and rename to avoid confusion)
df_multiindex = pd.concat((df, df_ix), axis=1).groupby(["Y", "M", "Desc"]).count()["Date"]
df_multiindex.name = "Vehicle counts"

The output is
print(df_multiindex)
Y     M   Desc
2022  04  Car     1
      05  Bike    1
          Bus     1
          Car     2
      06  Bike    1
          Car     2
      07  Bike    1
          Bus     1
Name: Vehicle counts, dtype: int64

redpajama_set_name: RedPajamaStackExchange


Dear Editor: It is sad that bad news sells better than good news.
Our subdivision at Green Village in Northport has been under attack in the news for the past few weeks. I spoke at the council meeting Monday night -- trying to update and set some records straight. My story about the good side of things got nowhere in The Tuscaloosa News.
Green Village is a mobile home subdivision -- not a trailer park. My address for years was Green Village Subdivision -- until 911 changed it to 6800 McFarland.
We were compared to a trailer park of 20 or less lots. They had about 40 police calls. We had over 700 for one year. We have 200 lots at Green Village -- no comparison.
They quoted us as having 10 or less people (homeowners) that lived on their lots. We have probably 10 homeowners that have been living there 25 years -- like myself. We have had a homeowners association for the 25 years I have owned my property there.
The city, the landlords and tenants are doing a great job in the cleanup program. It is looking like it did 20 years ago when all were homeowners and kept up their property. Please keep up the good work so that we can have that better quality of life that we had before and can have again.
In my opinion our property just happened to be in the right place at the right time (on U.S. Highway 82 west).


The Bakers were an obvious choice for the Last Supper since bread was an essential requirement for the institution of the Eucharist. No event in biblical history could be of greater significance in relation to the feast of Corpus Christi, which was a celebration of the Sacrament of the Eucharist in liturgical rite and procession as well as, at York, the plays. It is thus all the more unfortunate that, due to the loss of a leaf between lines 89 and 90, the central portion of the narrative with its representation of the blessing of the bread and wine is missing. The actions performed by Jesus at the table very likely were modeled on the gestures of the priest in consecrating the elements at Mass. This is supported by the stage direction that has Jesus perform the lavabo rite by washing his hands after the foot washing and in preparation for the main rite of the Passover meal. King points out that the lesson for Maundy Thursday described the foot washing, and that at vespers the York Missal quite remarkably specifies a mimed representation of the ritual meal: "table-cloths and wafers, with wine, are to be placed before the bishop and the others sitting together by the ministers of the church, as if to dine."1 It is hard to know how the actors were arranged for the scenes involved in the Bakers' pageant, but suggestions may be gained from entirely conventional mid-fourteenth-century painted glass in the choir of York Minster. In the Last Supper panel, Jesus is centrally placed and blessing with his right hand, while with the left he reaches out toward a dish on the cloth-covered table. The apostles are located on each side, except for Judas, who is in front of the table, as typically is the case so that he will be turned away from the viewer's gaze. A chalice and a ciborium, with hosts, stand on the table.2 At Great Malvern, a panel shows Judas receiving the Host and simultaneously stealing a fish, which he is hiding under the table cloth.3 The classic article on the play is by Mill, "York Bakers' Play of the Last Supper." The pageant is written in twelve-line stanzas.
4 feeste of Paas. The Pascal feast, Passover, which is to be hereafter rejected in favor of the new Easter feast and the regular celebration of the Mass. The Eucharist was presented by Love and others in terms of a sacramental mysticism (see Mirror, pp. 153–56).
7–8 Oure lambe is roste . . . / As Moyses lawe will lely lere. Prescriptions for the roast lamb are found in Exodus 12:3–10.
9–10 ilke man that has / Pepill in his awne posté. Implying the authoritarian family, with the patriarch as head of the family responsible for organizing the feast.
19 array you all on rawe. Perhaps the disciples took places standing by the table, but the foot washing will intervene before they actually are allowed to eat.
25–26 Of Moyses lawes here make I an ende / In som party. The extensive rules specified in the Old Testament are abrogated, and the new law of Christ will be substituted. For example, the lamb will no longer be required, nor the ritualistic way of eating it. But not all the old rules will be declared obsolete.
36 wasshed clene. The new law will declare that persons taking communion must be cleansed through penance and absolution.
40 Do us have watir. Embedded stage direction. In Mark14:13–14 and Luke 22:10 two disciples are sent into the town to meet Marcellus for the pitcher of water, but there is no time for anything so time-consuming. Marcellus will also bring a towel for the foot-washing.
47–50 Peter objects to having his feet washed by Jesus, as in John 13:6–9.
60 s.d. Tunc lavat manus. The foot washing is completed, and it seems that among the disciples only Peter took part in it in the pageant. Now is the preparation for the meal, which is to be an example for all future generations.
79 To whilke of you such fare schulde fall. The disciples have been talking inappropriately about precedence among themselves.
after 89 The missing leaf contained the Last Supper up to the moment when Jesus gives the sop to Judas. This is the detail depicted in the Great Malvern glass and very commonly in other works of visual art. In the popular Biblia Pauperum block books the event is aligned with Melchisedech's offering of bread and wine and with Moses in the desert who feeds himself with manna (pp. 81 and 83). In play production, as in depictions in the visual arts, it would probably have been the case that Judas was turned away from the audience so as not to make eye contact.
96 thou sittist nexte his kne. Spoken by James to John.
104–05 Now is tyme to me to gang . . . of newe. Embedded stage direction. Judas' exit is covered by the disciples seeming to be talking quietly among themselves.
116–23 Jesus' warning to the disciples. He especially has prayed for Peter, who will need to fend off the assaults of the devil — and, as indicated below in lines 132–37, will deny Jesus three times before the night is over.
142–43 All that in worlde is wretyn of me / Shall be fulfilled. All the ancient prophecies will be fulfilled and proven true.
144–47 I am the herde, the schepe are ye . . . to save. The brief parable of the shepherd and the sheep, told to the disciples by Jesus in Matthew 26:31–34 and Mark 14:27–31.
159 on twelffe seeges sitte schall ye. In heaven, the apostles are promised twelve thrones to sit beside Jesus at Judgment Day.
168–70 swerdis . . . Shall selle his cote and bye hym one. A rather confused adaptation of Luke 22:36 and 38. Andrew will report that they have two swords at line 176.
173–75 And stones to stynte all striffe. They are to carry stones for protection? The editor is indebted to a suggestion by Russell Peck, who wonders if this might not be an echo of the Temptation pageant in which the Fiend would tempt Jesus to turn stones into bread; here Jesus will turn bread into the body of Christ "Yoursellfe for to save" (line 174).
1 JESUS. So LTS, RB; Reg: Deus in JC's hand.
Above, top right of page in Reg: caret hic principio.
Pees. Reg: Large P sketched in.
2 Reg has all interlined; and here added by later scribe (replacing ?theryn).
60, s.d. Tunc lavat manus. Supplied by JC in right margin in Reg.
61 Reg has ascription by JC: Deus. This edition omits.
After 89 Missing leaf follows in Reg.
96 I hope. So Reg, LTS; RB: Jhon.
100 is. So LTS, RB; Reg: is is.
148 slayne. So RB; Reg, LTS: allone.
151 mende. So LTS, RB; Reg: mened.
173 stones. So Reg, LTS, RB; Kölbing: swordes.
177 Us. So LTS, RB; Reg: Vis.
187 At left in margin in Reg: Hic caret, in LH; JC has added novo loquela.
Footnote 1 King, York Mystery Cycle, p. 173, quoting the York Missal, 1:97, 101 (in translation).
Footnote 2 See YA, p. 71.
Footnote 3 See Rushforth, Medieval Christian Imagery, pp. 57–63, fig. 13.
Untill this house and till all that is here.
The feeste of Paas with frendis in feere.
As Moyses lawe will lely lere.
To hym and his meyné.
To roste a lambe and ete it clene.
Myselfe schall parte itt you betwene.
To the poure that purveyse none.
With tham that men schall craftely call.
Fro Cristis folke whatso befall.
Behoves to be wasshed clene.
In harte tham bus be clene and chaste.
Do us have watir here in hast.
And here a towell clene to taste.
My wordis schall noght be wroght in waste.
They schall be wasshen sone.
That dede schall noght be done.
Of my soverayne service to see.
Thou getis no parte in blisse with me.
Both hede and hande, beseke I thee.
What this werke will bemene.
And so schall ye all, bedene.
And so I am, all welthe to welde.
To wasshe youre feete as ye have feled.
Ilkone for to bede othir belde.
To greve whan I am gone.
Late loke now whils we dwell togedir.
Of such materes to melle.
Fayndyngis full ferse and felle.
This yonge childe for insaumpills seere.
That thou schall do, do sone.
Has betrayed hym to synfull seede.
We pray thee spire hym for oure spede.
JOHANNES & Domine, quis est qui tradit te?
Lord, who schall do that doulfull dede?
For bale myn herte may breste.
For here begynnes noye all of newe.
And certis thai schall noght wene it wrang.
That all his sawes sore schall hym rewe.
And tyte he schalle be tane.
And wisse hem whan I am gone away.
PETRUS & A, Lorde, where wilte thou lende?
Evermore in lyffe and dede.
That I schall with thee leve and dye.
Ellis mekill woo were we worthy.
That thou schalte fynde no fantasie.
Nor no meyné of myne.
Be putte to endles pyne.
Ye nedis non othir recours to crave.
Shall be fulfilled, for knyght or knave.
And socoure seke thameselffe to save.
Than schall youre myrthe be mende.
My comaundementis in ilke contré.
Even as my Fadir has highte itt me.
Than schall ye turne away with tene.
Thus bidde I that ye do bedene.
In lenghyng of youre liff.
Us with to save on sidis seere.
For fro all wathis I schall you were.
By this owre enemyes ordand are.
His bidding will I noght forbere.
Ay blessed schall thei bee.
Lord, who is the one who has betrayed you?


text:
"GPGPU Acceleration of Environmental and Movement Datasets" by Bird and Laycock
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GPGPU Acceleration of Environmental and Movement Datasets
Daniel Bird
Stephen Laycock
Due to the increased availability and accuracy of GPS sensors, the field of movement ecology has been able to benefit from larger datasets of movement data. As miniaturisation and the efficiency of electronic components have improved, additional sensors have been coupled with GPS tracking to enable features related to the animal's state at a given position to be recorded. This capability is especially relevant to understand how environmental conditions may affect movement.
Surveys that have been conducted in the field of visualisation for movement ecology have highlighted that tool-sets investigating movement within context are important [Andrienko and Andrienko 2013; Demšar et al. 2015]. Investigating movement within context allows for possible inference on why and where movement takes place [Nathan et al. 2008]. Due to the exceptionally large size of global datasets, visualisations are often calculated for a local area, which is viable for studies that only remain within a local area, but for large scale movement, such as migratory movement, it is more difficult. For example, [Gilbert et al. 2016] studied the migratory pattern of white storks, that migrate from Portugal to Morocco, to observe the effects of environmental factors, such as vegetation and land use.
Natalia Andrienko and Gennady Andrienko. 2013. Visual analytics of movement: An overview of methods, tools and procedures. , 3–24 pages. https://doi.org/10.1177/1473871612457601
Urška Demšar, Kevin Buchin, Francesca Cagnacci, Kamran Safi, Bettina Speckmann, Nico de Weghe, Daniel Weiskopf, and Robert Weibel. 2015. Analysis and visualization of movement: an interdisciplinary review. Movement Ecology 3, 1 (mar 2015), 5. https://doi.org/10.1186/s40462-015-0032-y
Nathalie I. Gilbert, Ricardo A. Correia, João Paulo Silva, Carlos Pacheco, Inês Catry, Philip W. Atkinson, Jenny A. Gill, and Aldina M. A. Franco. 2016. Are white storks addicted to junk food? Impacts of landfill use on the movement and behaviour of resident white storks (Ciconia ciconia) from a partially migratory population. Movement Ecology 4, 1 (dec 2016), 7. https://doi.org/10.1186/s40462-016-0070-0
Ran Nathan, Wayne M Getz, Eloy Revilla, Marcel Holyoak, Ronen Kadmon, David Saltz, and Peter E Smouse. 2008. A movement ecology paradigm for unifying organismal movement research. Proceedings of the National Academy of Sciences of the United States of America 105, 49 (dec 2008), 19052–9. https://doi.org/10.1073/pnas.0800375105
Movement Ecology
Visualisation Toolkit
We gratefully acknowledge the support of NVIDIA Corporation with the donation of the Titan Xp GPU used for this research. We would also like to thank Dr Aldina Franco for supplying the GPS data for the White Storks [Gilbert et al. 2016] and the discussions on the research.
SIGGRAPH 2019: Posters


text:
Diagnostics Developer Nanopore Wins Agtech and Food Startup Showcase
Bio Roundup: Amarin's Approval, FTC v. Illumina, a $120M Deal & More
Joel S. Marcus
Chairman, CEO, & Founder, Alexandria Real Estate Equities
Desh Deshpande
Founder and Chairman, Sycamore Networks
Alexander Wong
Managing Director, D.E. Shaw and Co., L.P.
Art Mellor
Software Engineer at Skelmir
Bill Clarke
Medical College of Wisconsin director of research commercialization
Carol Gallagher
Partner at New Enterprise Associates
Boston Tech Watch: Rethink Robotics, SquadLocker, Semcasting & More
Jeff Bauter Engel
There was a flurry of tech deals announced this week in Boston, including the acquisitions of Applause, Digital Lumens, and Dragon Innovation, and a $6 million investment in GNS Healthcare. Here are a few more deals you might have missed:
—Rethink Robotics raised $18 million from investors, part of a funding round announced last December that now totals $36 million, according to SEC filings. That brings Rethink's total venture capital haul to at least $148 million. The company makes robots that can collaborate with factory workers on tasks like assembly and testing.
—Semcasting, a North Andover, MA-based provider of data tools for marketing and advertising, acquired Orlando, FL-based Transparency AI for an undisclosed price. Transparency AI helps clients in the automotive industry measure the effectiveness of their online advertisements. With the acquisition, Semcasting now has around 60 full-time employees located at offices nationwide, a spokeswoman said.
—Warwick, RI-based SquadLocker received a $7 million Series B investment led by Causeway Media Partners, a spokesman said. Causeway's managing partners include Boston Celtics co-owner and CEO Wyc Grousbeck. Earlier SquadLocker investor James Lombardi also contributed to the funding round. Causeway managing partner Bob Higgins has joined SquadLocker's board, according to a press release.
SquadLocker's online tools help coaches and parents manage the process of designing and ordering youth sports apparel. The company has raised about $18 million from outside investors, according to the Boston Globe. SquadLocker co-founder and CEO Gary Goldberg has also put in $4 million, the Globe reported.
—Here's one that flew under the radar: Day Zero Diagnostics announced earlier this month that it closed a $3 million seed funding round led by Golden Seeds and Sands Capital Ventures. The startup, based at the Harvard Innovation Lab, wants to use genomic sequencing and machine learning tools to improve infectious disease diagnostics.
—Boston medical device firm Rebion raised nearly $2.2 million from investors, according to a new SEC filing. Formerly known as Rebiscan, the company says it has developed eye-scanning technology for detecting lazy eye and traumatic brain injury.
—CareAcademy closed a $1.7 million seed round led by Rethink Education, Lumina Foundation, and Techstars Ventures, according to multiple news reports. The Boston-based startup, which provides online education for professional in-home caregivers, participated in this year's Techstars Boston accelerator program.
Boston Tech Watch: Buoy Health, Cambridge Crops, Polarity & More
Boston Tech Watch: Nike, Cybereason, Care.com, LaunchByte & More


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Death row inmates sue to stop importation of drug used in executions
Six death row inmates have filed a lawsuit seeking to prevent the Food and Drug Administration from importing a drug used in executions that's no longer available in the United States.
Hospira, the only U.S. manufacturer of sodium thiopental, an anesthetic used as part of the lethal three-drug cocktail, announced last month that it would stop making the product, saying it never intended it to be used to kill people.
But the FDA continues to allow states to import "bulk amounts" of the drug for use in lethal injection without vetting it to ensure it meets regulatory standards, the lawsuit states.
"The imported thiopental in question has not been listed with FDA, was manufactured by foreign companies that have not registered with FDA, and was exported by a wholesaler located in the United Kingdom," claims the lawsuit, which was filed Wednesday in U.S. District Court in the District of Columbia.
"FDA has not determined whether the thiopental being imported into the United States is safe and effective, whether the imported thiopental is adulterated, or whether the imported thiopental is counterfeit."
The lawsuit, filed on behalf of inmates in Arizona, California and Tennessee, seeks an injuction barring future imports of the drug and removal of supplies of the drug already in possession of state governments.
An FDA spokesman declined to comment, citing pending litigation.
The shortage in the United States of sodium thiopental, which is also used as an anesthetic for brief surgical procedures, has forced states to look outside the country for a supply or come up with alternatives, and not without controversy.
In December, Oklahoma used an anesthetic commonly used to euthanize animals to execute John David Duty for killing a cellmate.
Post by: CNN's Emanuella Grinberg
Filed under: Arizona • California • Death Penalty • Lawsuit • Tennessee
I read the story several times – what state was the law suit filed at? And.....if that specific drug is not available, there are plenty of others with the same efficacy. Let go. Too many people on death row for too long.
February 7, 2011 at 9:33 am | Report abuse |
@Jason and @David...it's "Thou shalt not commit MURDER". If you continue reading, you will find scriptures where one is required to kill. 'Killing' and 'Murdering' are NOT the same thing. G-E-T a clue already.
February 7, 2011 at 10:45 am | Report abuse |
Guess it's time to bring back hangings and firing squad
michael mobley
No One Should Jugde No Man Nor Woman. Ever One Deserve A Second Chance. Put One Of The Congress People Kid On Death Row And C Wat The Results B. C If They Make It To Prison. They Want Just Because Who The Parents R. Thats How The System Work.
March 16, 2011 at 9:41 am | Report abuse |


These pancakes look absolutely delicious and I know serving them would be a better ides, but STILL!
Wow! Those ARE a great way to start your day!
Valentine's day is definitely controversial. Jason and I are always joking that it's a Hallmark holiday but with this year, I'm particularly excited about all the Valentine's day-themed things I can make, like these beautiful pancakes!
I think I'm going to need batch upon batch of these on V-day. I figure as far as stress eating goes…these are the least of many evils, right?
If only any of my friends had kids…I would TOTALLY babysit!
I can think of a better way… hee, hee. I know bad Greg. But these look like a wonderful way to enjoy the morning.
It's hard to imagine any version of red velvet goodness that wouldn't make my tastebuds wonderfully happy. These do look like just the ticket!
Combining my favorite cake with my favorite breakfast…perfect!
I LOVE valentine's day and make it all about the family love. These pancakes would be perfect for my boys! Your family is very lucky to have you, Geni!
I definitely wan to try this recipe once eveyone in my family is over the stomach flu and gains an appetite. I love your recommendations, anyone can comeover anytime to watch my kids, bring some cookies and a Disney movie and they'll love ya! My hubby and I need some r&r time! 🙂 Great post as always Geni! Have a great Sunday!
These remind me of when my kids were little and I'd turn everything red that they ate for Valentine's day and green for St. Pattys!! So I guess my gifts on this day are always food related too!! And even though I'm single, I'm still enjoying every part of this Valentine's day!
I am so tempted. Yum ….
My daughter loves red velvet, I need to make these, she'll love them!


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Colonial - 134 Arnold AV Cranston, RI 02905 now has a new price of $279,000!
Stately 3 bedroom Colonial in the heart of Edgewood! Large master bedroom, with an outside deck, 2 good size guest rooms and hardwoods throughout. Heating system & hot water tank (gas), windows and roof all replaced within the last 8 years. Walk to both, the RI Yachting Club and the Edgewood Yacht/Sailing Club. The property is also a short drive to Pawtuxet Village and many shops and fabulous restaurants.
Colonial - 25 Dixwell AV Cranston, RI 02910 is now new to the market!


Welcome to our members only area where we have over 70 training videos, worksheets, articles and manuals on all things web. From eBooks on marketing to complete WordPress training manuals there's plenty to see.
Every website we design includes a minimum of 2 hours face-to-face training to show you the ropes and enable you to manage and update your own website. However, we understand that sometimes that isn't enough, and especially over time, some tips and tricks may get forgotten.
That's why we've created this free resources and training site for our clients both large and small.
If you would like to access the Client Portal and you haven't been issued a password please get in touch hello(at)wepushbuttons.com.au.


text:
European Commission backs Takeda's pioglitazone-containing drugs
Takeda Pharmaceutical Co says that the European Commission has adopted the European Medicines Agency's view that the Japanese drugmaker's pioglitazone-based drugs, notably Actos, are still valid treatments for type 2 diabetes.
The move backs the EMA's Committee for Medical Products for Human Use opinion in October, which included label changes and clarification on the product indications for pioglitazone-containing medicines, eg Actos (pioglitazone) and Competact (pioglitazone/metformin). This concludes the European review which was initiated in March 2011 following the publication of data from a retrospective study which appears to indicate a slight increased risk of bladder cancer with the Takeda treatments.
Last summer, the CHMP concluded that pioglitazone-containing medicines remain a valid treatment option for certain patients with type 2 diabetes. While a small increased potential risk of bladder cancer has been associated in patients taking these drugs, the CHMP said this risk could be reduced by appropriate patient selection and updated contraindications and warnings in the product labels.
Takeda said it "continues to remain confident in the therapeutic benefits of pioglitazone as an important treatment for type 2 diabetes".
Mylan sued over generic Actos/metformin combo
Meantime, Takeda, Watson Pharmaceuticals and Andrx Labs have sued Mylan for patent infringement in connection with the latter's plans to market a generic version of Actoplus Met XR (pioglitazone/extended-release metformin).
In a statement, Mylan said it was the first to file "a substantially complete Abbreviated New Drug Application" to sell all strengths of the drug", thus qualifying for 180 days of marketing exclusivity upon final approval from the US Food and Drug Administration.
EMA warns about Actos, backs Champix, restricts Pandemrix
France and Germany pull Takeda's Actos over cancer link
http://www.takeda.com
http://ec.europa.eu


Rich and aromatic crackers made with extra virgin olive oil and real wild Porcini Mushrooms.
Crustaceans, egg, celery, fish, mustard, peanuts, mollusc, soya, sulphites and lupin.
Wheat Flour, Extra Virgin Olive Oil (11%), Dried Mushrooms (5%), Sea Salt, Wild Porcini Mushrooms (2%), Black Pepper, Natural Flavouring (Milk).


text:
Xander Ramone
Xander has a hyperactive imagination, a creative/professional writing degree, DSLR camera, and a massive vinyl collection -- and knows how to use them. He's also a sucker for Shakespeare, "Bob's Burgers" and the cinematic works from the Criterion Collection. Some radio show host once told Xander he couldn't be a "jack of all trades." He thought that was rather funny and felt the wrath of "Snark Mode." Though Xander knows how to use "Snark Mode," his wife Janice is the master. When it comes to music, Xander doesn't have a favorite band or genre. He listens to everything and his mind changes every five seconds. Xander has been a photojournalist for over a decade and his work has been featured in Rolling Stone, People, Spin, The Guardian, Daily Mail, MSN, and Alternative Press. When Xander is not busy, he spends time with his wife Janice and their three "fur babies." (2 Cats 1 Giant Malamute)... And no, Xander does not own the record he is holding in his profile photo. (UPDATE: Someone sent it to him as a gift so now he does)
'Bullfighters Only' Brings Its A-Game to Central Washington [PHOTOS[]
Bullfighters Only made a stop at the Toyota Center in Kennewick this past weekend. BFO is the revolutionized version of American Bullfighting -- rodeo style meets extreme sports.
Shooter Jennings Sells Out Zillah's Perham Hall [PHOTOS]
Jennings -- son of the late great Waylon Jennings -- brought his A-game, performing songs off of his newest album, "Shooter."


text:
Q: i can't get e.values (for sheets) or e.responses (for Forms) to work I want to fill out a google form on a jobsite and when I submit, I need a pdf containing the submitted info to be emailed to my office.
Here's where I'm at so far:
 // Get template from Google Docs and name it
var docTemplate = "12vZhIPt1CkWPCCN3jRE1Kpbp0dBW3key-4I_Stz0vNc";
var docName = "JobsiteInspectionReport";

// When Form Gets submitted
function onFormSubmit(e) {
//Get information from form and set as variables

var variablename = "static entry or form value"
var email_address = "mike.onlineconstruction@gmail.com, mwfoshee@gmail.com";

//  Use this section to assign static values

  var jobsite = "test data";
  var date_time = "test data2";
  var submit_name = "test data3";
  var weather = "test data4";
  var temp = "test data5";
  var super_name = "test data6";
  var job_num = "test data7";

// Use this section when attached to form

  var jobsite = e.responses[2];
  var date_time = e.responses[3];
  var submit_name = e.responses[4];
  var weather = e.responses[5];
  var temp = e.responses[6];
  var super_name = e.responses[7];
  var job_num = e.responses[8];

// Use this section when attached to a sheet

  var jobsite = e.values[2];
  var date_time = e.values[3];
  var submit_name = e.values[4];
  var weather = e.values[5];
  var temp = e.values[6];
  var super_name = e.values[7];
  var job_num = e.values[8];

  //General Items

// Get document template, copy it as a new temp doc, and save the Doc's id
var copyId = DriveApp.getFileById(docTemplate)

.makeCopy(docName+' for '+jobsite)
.getId();
// Open the temporary document
var copyDoc = DocumentApp.openById(copyId);
// Get the document's body section
var copyBody = copyDoc.getActiveSection();

// Replace place holder keys,in our google doc template
copyBody.replaceText('keySiteName', jobsite);
copyBody.replaceText('keyDateTime', date_time);
copyBody.replaceText('keySubmitName', submit_name);
copyBody.replaceText('keyWeather', weather);
copyBody.replaceText('keyTemp', temp);
copyBody.replaceText('keySuperName',super_name);
copyBody.replaceText('keyJobNum', job_num);

// Save and close the temporary document
copyDoc.saveAndClose();

// Convert temporary document to PDF
var pdf = DriveApp.getFileById(copyId).getAs("application/pdf");

// Attach PDF and send the email
var subject = "Jobsite Inspection Report";
var body = "Here is the Jobsite Inspection Report for " + jobsite + "";
MailApp.sendEmail(email_address, subject, body, {htmlBody: body, attachments: pdf});

// Delete temp file
DriveApp.getFileById(copyId).setTrashed(true);
}

I have installed the onFormSubmit trigger for both sheets and forms and have successfully sent emails with attachments from scripts in both forms and sheets by deleting the e.values (in the sheets script I use e.response when the script is installed on a form) and assigning static values to the variables (see the section of code marked "Use this section when attached to form"). I receive an email, on the correct template with the correct static data.
When I try to use the e.values or e.responses section, nothing. I don't even get an email or error.
I did get an email (several in fact) a few hours later stating "TypeError: Cannot read property "2" from undefined. (line 25, file "Code")" I'm thinking the e.values[2] is my problem (or the way it's formatted, or more specifically defined). The script appears to be looking for the form?

A: The submit event object needs further work before you can access it the way you are indicating. If you want to access the responses as a simple array you'll need to transform from a FormResponse object and the indiidual ItemResponse values
function onFormSubmit(e) {

  // you need to add this line to add offline event object access auth
  // run once in script editor - you could delete it after if you want
  var f = FormApp.getActiveForm();

  // test for response and use static if none exists
  var responses = [
    "test data",
    "test data2",
    "test data3",
    "test data4",
    "test data5",
    "test data6",
    "test data7"
  ];

  responses = (e && e.response) ?
    e.response.getItemResponses()
              .map(function (r) {
                return r.getResponse();
              }) : (e && e.values) ? e.values : responses;

  // snip //

  var jobsite = responses[2];
  var date_time = responses[3];
  var submit_name = responses[4];
  var weather = responses[5];
  var temp = responses[6];
  var super_name = responses[7];
  var job_num = responses[8];

  // snip //

}

A: I finally gave up and started over and and it works! the script is the same as the one listed above (without all the commented sections I used while troubleshooting). the only thing I can say might be different are the setting on the form itself. For anyone having this same issue, I would check the settings on the form. On the form that works, the setting "Allow responders to edit responses after submitting" is un checked where as I think this was checked on the first form I used. If that's not it, I'm still at a loss.


text:
Home » Lou Williams On Why He Stopped Caring About Fashion: "I Bought A Gucci T-Shirt For $400 Wore
Lou Williams On Why He Stopped Caring About Fashion: "I Bought A Gucci T-Shirt For $400 Wore
Credit: Raj Mehta-USA TODAY Sports
Atlanta Hawks star Lou Williams shared his two cents on fashion, and safe to say, the decision he made to stop buying expensive and fashionable clothing helped him save money.
The 3-time NBA Sixth Man of the year also explained what he preferred instead of the luxury brands and that comes as a surprise to most who are under the impression that high-profile athletes lead a lavish lifestyle.
On a social media post that shared Williams' take on fashion, he's seen explaining that he prefers something minimalistic and cheaper.
"I stop caring about fashion maybe 5-6 years ago. I bought a Gucci t-shirt for $400 wore it once, washed it, and decided I wouldn't make a habit of buying that stuff. Polo tees. Slim fit medium. 3 for $40."
Lou Williams' Down To Earth Nature Has Been Documented Earlier As Well
The idea of fashion and the simplicity of his lifestyle hasn't been the first instance of the 35-year-old's rather down-to-earth nature. Per a Sportscasting report, the guard hosts a summer camp at his high school alma mater, South Gwinnett High School every year, and that was his way of doing something for the children.
"Being a youth, I really didn't go to a lot of after-school activities. I didn't have the resources or funds in my family," he explained. "To have the opportunity to do it for these kids at an affordable price means a lot to me."
On the season front, Williams, who re-signed with Atlanta in August 2021 will suit up as they look to make another postseason bid. In his previous season with the side, he averaged 6.3 points, 1.9 assists, and 1.6 rebounds in 56 games.
The previous season saw them lose to the Miami Heat in the first round. The 2020-21 season was a good run for the side as they trounced the New York Knicks 4-1 in the first round, and forced a Game 7 against the Philadelphia 76ers to win 4-3. They lost to the Milwaukee Bucks in the Conference finals.
The Hawks will start their campaign at home against the Houston Rockets on October 19.
Read More: Lou Williams On Why He Stopped Caring About Fashion: "I Bought A Gucci T-Shirt For $400 Wore
Topics: Atlanta Hawks Bought Caring fashion Gucci Lou Lou Williams NBA Media Stopped tshirt Williams Wore
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text:
JEFF, WHO LIVES AT HOME
By	Tom Hall
(Distributed by Paramount Pictures, Jeff, Who Lives At Home is now available on DVD + UltraViolet , Blu-ray + UltraViolet , and at Amazon Instant . It opened theatrically on Friday, March 16, 2012. NOTE: This review was first published on September 14, 2011, in conjunction with the film's world premiere at the 2011 Toronto International Film Festival. Visit the film's official website to learn more.)
A look at the Duplass Brothers' body of work finds both striking stylistic similarities across the films and storytelling that continues to grow with each project. In their new film Jeff, Who Lives at Home, the brothers' signature techniques—a handheld camera that zooms in on the faces of their actors, a tightly circumscribed group of characters—remain, but their generosity has grown by leaps and bounds. Whereas a film like The Puffy Chair took great pleasure in the unresolved suffering of its protagonists, Jeff is interested in creating harmony in the universe and allowing its characters' dreams to come true in unexpected, moving ways.
Jeff (Jason Segel) is a thirty-something stoner living in his mother's (Susan Sarandon) basement. His ambitions lie outside of the world of material concerns; the film's opening monologue, which features Jeff declaring his love for the elegant plot machinations of M. Night Shyamalan's film Signs, performs the neat trick of simultaneously highlighting Jeff's seemingly misguided understanding of fate and the sincerity of his world view. When his mother asks him to visit the hardware store to get the materials required to fix a broken shutter, the act sets into motion a series of unlikely coincidences that lead Jeff into an encounter with his alpha male, materialistic brother Pat (Ed Helms), whose decision to buy a sports car creates a rift in his marriage. When their lives finally converge with that of their mother, destiny shows its hand and allows the entire family a chance at reconciliation.
As filmmakers, Jay and Mark Duplass each have unique gifts; Jay's cinematography (generous in the way it uses actors' faces to deliver emotions) and staging are in top form here, highlighted by a surprising car chase that shows a real gift for action sequences. This scene is by far the most sophisticated (and likely expensive) they've made, but it still feels perfectly at home in the film because it is at once harrowing and funny. Mark Duplass has always found a way to give his actors hilarious pieces of on-screen "business," creating characters and situations that are served by small details that deliver wonderful, telling information. Here, there is Pat's goatee and his driving gloves, as well as a great moment where Jeff, compelled by fate to follow the "signs" that are appearing all around him, apologetically hitches a ride on the back of a candy truck.
But in the end, what is clearly separating the Duplass Brothers from their peers in the independent filmmaking community—though this is admittedly a star-studded Paramount picture, their process and spirit remain indie—is the way in which they use comedy as a way to reinforce their humanism. It is this belief in and support of everyday characters and their dreams and desires that finds the brothers' films becoming more and more purposeful. Whereas their earlier movies found comedy in self-certainty (The Puffy Chair), ambition (Baghead) and oedipal jealousy (Cyrus), Jeff, Who Lives At Home rewards its titular protagonist not just by giving him dopey ideals, but by making those dopey ideals come true through a profoundly important act that re-frames everything we might have otherwise assumed. By turning Jeff into a hero, the Duplass Brothers signal their own move into another world.
— Tom Hall
2011 Toronto International Film FestivalBagheadCyrusDuplass BrothersEd HelmsJason SegelJay DuplassJeff Who Lives At HomeM. Night ShyamalanMark DuplassSignsSusan SarandonThe Puffy Chair
Tom Hall is the Artistic Director of the Sarasota Film Festival (2005- present) in Sarasota, FL and Programming Director at FILMnewport in Newport, RI (2009-present). Formerly, Hall was Programmer for The Nantucket Film Festival in Nantucket, MA (2002-2005) as well as a former Director of New Media for Bravo/The Independent Film Channel (1997-2000). He has also worked in the Industry and Guest Services Offices at The Hamptons International Film Festival (2002-2003). In January of 2010, Tom was named one of Spring Board Media's 20 under 40 in Film. Tom has directed short films for Bob Mould's Carnival of Light and Sound Tour and is a member of the indieWIRE blogging community with his blog The Back Row Manifesto. A graduate of the University of Michigan ('94), Hall resides in Brooklyn, NY with his wife and son.
Fifedog2
Let me get this straight. What is separating the Duplass Brothers from their peers in the independent filmmaking community is the way in which they use comedy as a way to reinforce their humanism? It is this belief in and support of everyday characters and their dreams and desires that finds the brothers' films becoming more and more purposeful.
Do you even know what you're talking about?
This review is amateur hour.
Pingback:	HOME VIDEO PICKS – Hammer to Nail


text:
Edinburgh, Fife & East selected
Glasgow & West
Edinburgh, Fife & East Scotland
Cyclists in 'silent protest' over Edinburgh tram track death
https://www.bbc.com/news/uk-scotland-edinburgh-east-fife-40179483
Media captionProtestors carried placards and rang their bicycle bells as a mark of respect for Zhi Min Soh
Cyclists have held a minute's silence for a student who was killed by a tour bus after her wheel reportedly became trapped in a tram track.
Zhi Min Soh, 23, was struck on Edinburgh's Princes Street, at its junction with Lothian Road, at 08:30 on Wednesday 31 May.
Pedal on Parliament held "a short, respectful protest" at the scene exactly a week on from the accident.
Cyclists have previously branded the tracks "an accident waiting to happen".
Ms Soh, from Kuala Lumpur in Malaysia, was a medical student at Edinburgh University.
'Angry and saddened'
She was described as "talented and thoughtful". The university said it had lost "a bright star of the future".
A Pedal on Parliament statement said: "We and every other cyclist we have spoken to personally and online are angry as well as saddened at this death.
Image copyright Police Scotland
"Edinburgh's tram tracks have been described as an accident waiting to happen from the moment they were unveiled.
"As a result, hundreds of cyclists have been injured from falls on the tracks, and thousands more have had close shaves, putting many off cycling those roads at all.
"This is a sorry record for a city that aspires to be the most cycle-friendly in Scotland. We should not have had to wait for a young woman to die for there to be ministerial action, and a review."
At the protest Megan McHaney told BBC Scotland that she had broken her foot after cycling across a tram line in Edinburgh 18 months ago.
"My wheel slipped on the tracks and it slid out from under me and in that process my foot got mangled," she said.
Image caption Megan McHaney was involved in a cycling accident on a tram line.
Since then she has had two operations as a result of the accident and she is still not fully recovered.
"I came here today because the council has known for a long time that these tracks are not safe for cyclists," she said.
The cyclist added: "We have been waiting for someone to die and sadly somebody did and it's needless."
Chris Playfoot said he arrived at the scene of last week's fatal accident shortly after it happened.
"This is my regular cycle route," he said. "I came here maybe 10 minutes after it happened. It was pretty awful."
Image caption Henry Whaley said he was "saddened" by the death of the cyclist last week.
Another cyclist, Henry Whaley, said he wanted to see cyclists "segregated" from other road traffic, for their own safety.
He added: "More people will cycle if there are safe routes and people who do cycle will be safer if they're away from motorised traffic.
"Segregated routes are the way that we can make the city safer for people who travel around by bike."
The protest comes after lawyers claimed warnings about the safety risks which tram tracks in Edinburgh pose to cyclists have been ignored.
City of Edinburgh Council said it has made "every effort" to raise awareness about the tracks.
Edinburgh tram track death cyclist named
Edinburgh tram track safety warnings 'ignored', say lawyers
Edinburgh tram track injuries cost NHS more than £1m
Cyclist dies after being hit by minibus following Edinburgh tram track fall
Lawyers warn of cyclists' tram risk
Video Cyclists protest over Edinburgh tram tracks death
Pedal on Parliament
Rabbie's Tours
Thompsons Personal Injury Solicitors Scotland
In pictures: Harry and Meghan's life together
Nigerian civil war remix of a theatre classic


text:
Playing Protein Hide and Seek
LIGHTING THEIR LOCATIONS:© 2002 Cold Spring Harbor PressImmunolocalization of epitope-tagged proteins. (A-E) represent cells containing HAT-tagged proteins stained with the DNA dye, DAPI, and a monoclonal antibody against hemagluttinin, α-HA. At right the images are merged. (F-J) indicates cells carrying V5 tagged proteins. The bar equals 2 μm.Aliens sifting through the remains of a lost human civilization might puzzle over the function of a ladle. But if found in a room associate
LIGHTING THEIR LOCATIONS:
© 2002 Cold Spring Harbor Press
Immunolocalization of epitope-tagged proteins. (A-E) represent cells containing HAT-tagged proteins stained with the DNA dye, DAPI, and a monoclonal antibody against hemagluttinin, α-HA. At right the images are merged. (F-J) indicates cells carrying V5 tagged proteins. The bar equals 2 μm.
Aliens sifting through the remains of a lost human civilization might puzzle over the function of a ladle. But if found in a room associated with food preparation, its purpose would become clear. Similarly, a protein relegated to mitochondria, or another specific cellular location, might have a mercifully short list of possible functions. This guilt by association, or more formally, protein localization, plays an important role in assigning function to newly identified genes and proteins. "Localization helps you predict gene function. ... If you're in the nucleolus, you're likely to be carrying out a function that is nucleolar-specific," says Charlie...
HUNTING METHODS
Snyder used two tagging methods, producing multiple alleles for most genes tagged and corresponding strains where the localization patterns of the proteins could be assessed. The group cloned 2,085 yeast open reading frames into vectors containing the inducible GAL1 promoter and a V5 epitope allowing antibody visualization. They also created 2,958 mutants through random insertion of a transposon carrying a hemagglutinin epitope traceable by antibody. Overlap between the two methods meant that they could directly visualize the localization pattern of 3,565 proteins.
Working with human HeLa cells, Mann and colleagues took an entirely different approach to a similar problem. They biochemically purified nucleoli and used mass spectrometry to identify associated proteins. In this study, they identified 271 nucleolar proteins, the largest survey of the nucleolus at the time. More than 30% of these were encoded by novel or uncharacterized genes. When their work began, scientists were just starting to suspect that the nucleolus has functions beyond ribosomal-subunit biogenesis. " [We] showed that protein complexity was unexpectedly high, and that ties in with the idea of a pluripotent nucleolus," says Mann. In addition, cells treated with actinomycin D, an inhibitor of transcription, displayed unexpected changes in protein localization; this indicates a dynamic protein complement that may change in response to metabolic conditions.
A CELLULAR ADDRESS BOOK
The differing proteomic approaches each have advantages and disadvantages. Mass spectrometry can allow contaminating proteins to remain in the mix of purified proteins and may, therefore, miss proteins present at low levels. Mann's group employed clever computer techniques to largely overcome this problem (see p. 32).
Also, certain organelles are more amenable to purification; Mann chose the nucleolus precisely because it is easily purified. "The proteomics technology was kind of bleeding edge ... so we didn't want to also push the organelle purification technology," he says.
Alternatively, the cell biology approach (tagging proteins with a marker such as a hemagglutinin epitope or green fluorescent protein [GFP]) eliminates most concerns about contaminants and low-level proteins and can provide more detailed suborganellar localization data. But, small proteins are less likely to be tagged than larger proteins, and insertions can disrupt sequence-based localization signals, which lead to false cytoplasmic staining patterns.
Tagging creates a set of yeast strains that are useful for genetic experiments and studies of protein localization under different conditions, whereas biochemistry creates a set of purified proteins that are useful for a different range of future experiments. In general, researchers agree that both methods have advantages. "The neat thing is that these are two very different methods of attacking a similar problem, and it is nice to come at it with a sort of orthogonal approach because then you feel really confident about the data," says Toronto's Boone.
These large-scale localization projects offer a largely unbiased look at proteins' cellular addresses. Boone says that after the yeast genome had been sequenced, researchers realized they had studied only a small subset of its 6,000 genes. "We tend to study things that we think are hot like signal transduction, or cell-cycle regulation. ... The large-scale projects can, in a nonbiased way, add experimental evidence for a large number of genes," says Boone.

TAGGING STRATEGIES:

(A) Researchers cloned yeast open reading frames into an expression vector containing a GAL1 promoter, a V5 epitope tag, and a polyhistidine tag. Cloned genes were overexpressed with a V5 tag. (B) Modified bacterial transposons carrying three copies of the hemagglutinin epitope tag were randomly inserted into yeast genes at their native genomic loci. The trans-posons contain a promoterless lacZ reporter allowing for selection of in-frame insertions and can be shortened through Cre-lox recombination.
Nevertheless, an ongoing concern in large-scale, high-throughput studies is the great potential for false-positive and false-negative results. "It is the nature of high-throughput experiments," says Eric Phizicky, University of Rochester Medical Center in New York. But the growing number of large-scale studies provides a filter for cross validation. Localization is just one of a number of huge data sets coming out of the yeast community. "The localization sets are a crucial part of the bigger picture," says Phizicky.
Snyder says that the localization data provide one of the best filters available for sorting out the noise in other large-scale projects, such as the two-hybrid and TAP-tagging protein-protein interaction studies. "If you superimpose the localization data and only count those [proteins] that are roughly in the same compartment, the noise filters out dramatically," he says.
Protein-localization sets also allow access to data not easily obtained. "Every time you have to do a new technology in a lab there is a steep learning curve," says Phizicky. He says he wouldn't attempt to localize proteins, but the data help his research. "One of the proteins that is related to proteins we work on turns out to be [unexpectedly] attached to the mitochondria. ... [It] opens up new avenues of research," says Phizicky.
The Snyder lab also benefited from surprising results: They found far more proteins in the bud neck than previously appreciated. One of those proteins was a kinase, Hsl1, that postdoc Yves Barral figured might be monitoring a cell-cycle G2/M checkpoint he was trying to dissect. "It really broke open the whole thing in telling us how this signaling pathway worked and probably saved us years of work," says Snyder.
FURTHER EFFORTS
Since Snyder's effort, researchers Erin O'Shea, Jonathan Weissman, and their respective labs at the University of California, San Francisco, completed a similar project using a C-terminal GFP tag. That gave researchers a view of endogenous protein levels and localization in live cells; however, sensitivity issues may exist, such as missing proteins expressed at low levels.3 Combined, the two approaches now provide nearly complete experimental coverage of yeast protein localization.
On the human nucleolus, Mann says they have recently improved their techniques, employing more automation and improved instruments to increase their throughput. "Now, we have identified more than twice the number of [nucleolar proteins]," he says.
Researchers can now envision protein-localization evaluations in more detail, such as at the suborganellar level, and in more dynamic ways, comparing protein patterns at different developmental stages and under different environmental conditions. Moreover, protein-protein interactions may come into play. "If you have tools to look at the localization of a single protein, then in theory you have the tools to look at the colocalization of the pairs of proteins," says Boone.
Mann has already made progress identifying proteins in other human organelles and structures such as mitochondria and centrosomes. The yeast researchers fully expect that their studies are paving the way for similarly comprehensive studies in human cells. "If we could take a human cell to the same level of annotation [as yeast], that would just be incredible," says Boone.
Mignon Fogarty mignon@welltopia.com is a freelance writer in Santa Cruz, Calif.


I decided to do the Fall 10 x 10 Challenge to help condense my wardrobe and see what I actually wear. Starting in February of this year I decided to be an ethical consumer and I intend to be. I stopped shopping from fast retailers and I've just started my ethical wardrobe. Next, I purged my closet of old clothes or clothes I had not worn in a long time and donated or tried to re-sell all my clothes. With that said a lot of the pieces in my closet are fast fashion bought in the past.
The point of buying ethical fashion is not to get rid of everything you have and buy all new ethical clothing items, it is to love what you wear and actually wear the clothes you own. Eventually my wardrobe will be all ethical items as I transition and buy new pieces to replace the fast fashion items.
My key to getting this Fall 10 x 10 capsule to work is by layering my Nudy Patooty Cropped Undershirt or my Nudy Patooty Signature Seamless Undershirt to keep my clothes sweat free and clean longer. Nudy Patooty lets me wear my clothes more than once before washing. This is helpful well working with a capsule as a only have a limited number of pieces to work with.
Another great blogger I follow is Caroline Joy Rector from Un-fancy. Her blog focuses on mindful style and wearing and styling seasonal capsule wardrobes. Her and Lee from Style Bee often collaborate every season to share their 10 x 10 creations.
We welcome you to share your thoughts about the Fall 10 x 10 Challenge and if you've ever done it or are considering doing it in the comments below. We would love to start a conversation with you!


The amphibious transport dock ship USS New Orleans (LPD 18) transits the Gulf of Aden. New Orleans is part of the Boxer Amphibious Ready Group and, with the embarked 13th Marine Expeditionary Unit, is deployed in support of maritime security operations and theater security cooperation efforts in the 5th Fleet area of operations.
BALI, Indonesia (NNS) -- Sailors and Marines from amphibious transport dock USS New Orleans (LPD 18) and the 13th Marine Expeditionary Unit (MEU) anchored off the coast of Bali, Indonesia, Aug. 7-12.
COLOMBO, SRI LANKA (July 25, 2016) The Commanding Officer of the amphibious transport dock ship New Orleans (LPD 18) visited the Commander, Sri Lankan Western Naval Area Command July 25.
COLOMBO, Sri Lanka - USS New Orleans (LPD 18) with embarked 13th Marine Expeditionary Unit (MEU) arrived in Colombo, Sri Lanka, for a port visit, July 24.
COLOMBO, Sri Lanka - The USS New Orleans (LPD 18) with embarked 13th Marine Expeditionary Unit (MEU) arrived in Colombo, Sri Lanka for a port visit, July 24.


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text:
Meaningful Relationships
My first experience of engaging in pastoral ministry in Japan was at a residential center, run by the Daughters of Charity of St.
Small Pentecosts
You might call me a slow learner.
Learning to Be a Missionary
By Fr. Nilton Iman
My name is Nilton Iman, and I am a priest of the Diocese of Chimbote, a land blessed with the blood of the first martyrs of my country, Peru.
A Short-Term Mission Experience in the Pacific
By Patrick O'Dwyer
Fiji for me will always be one of the most wonderful places in the world.
Changed for Good
By Emmanuel Trocino as told to Stephen Awre
Welcome to Peru, Land of the Incas (Land of the Kings). Many would say that you've never really been to this resplendent country until you've set foot in the majestic and…
By Teakare Betero
My name is Teakare Betero, age 28, and I have been with the Columbans for five years now. At the moment I am studying at the Pacific Regional Seminary.
On Accepting and Being Accepted
By Paula Matakiviwa
In 2007 my father talked to me about the possibility of going overseas as a lay missionary.
Responding to the Need
By Saeko Yamaguchi
I came to live in Fujisawa after getting married 58 years ago. I have been fortunate with my health.
Mission to Share with Others
We rejoice in the great gift of life. But as we grow older this gift is overshadowed by experiences of illness, pain, and loss.
"I Just Want to Share My Story and Know Someone Cares"
By Sr. Abbie O'Sullivan
It has been nine years since I started working as a personal counsellor in a Boy's Secondary School in Dublin, Ireland, and also nine years since I started working in The…
Weakness and Failure Lead to New Understanding
By Columban Fr. Barry Cairns
Shusaku Endo, a Japanese novelist, died in 1996–almost 20 years ago. In Japan he is still featured regularly in television programs, magazine articles and exhibitions.
Cuzco's Children
Fr. Youngln Kim
Editor's Note: On Monday, November, 23, 2015, Columban Fr. YoungIn Kim celebrated the Feast Day of St. Columban in Cuzco, Peru.


text:
Ground Work 2022: Meet Illinois Farmer Lynn Rohrscheib
USSOY.org
Throughout the 2022 growing season, USSOY.org will provide regular Ground Work updates from several U.S. soybean farmers around the country. Follow their updates, #GroundWork2022, to learn about their farms and commitment to producing a reliable, sustainable supply of high-quality soy.
Lynn Rohrscheib shadowed her father and her grandparents as she grew up on her family's farm in Illinois, in the heart of the U.S. Midwest.
"When I was young, I was probably more in the way than anything," she laughs. "But the more I got involved in the farm, the more passion I developed."
That passion fueled Lynn to continue farming on weekends during college as she earned a degree in plant and soil sciences. She is now part of the ninth generation of farmers in her family, and the fifth generation to farm in their current location, about 225 km, or 140 miles, south of Chicago, Illinois, and about 175 km, or 110 miles, west of Indianapolis, Indiana.
Today, Lynn farms with her parents, Vernon and Mary, and her younger sister Stacey. Together, they farm about 2,955 hectares, or 7,300 acres, of soybeans and corn. Their fields cover a large footprint, as far as 56 km, or 35 miles, both north and south of their home farm. They farm up to 24 km, or 15 miles, to the west and 48 km, or 30 miles, to the east, which includes fields in the neighboring state of Indiana.
In addition to farming, Stacey teaches agriculture for a local school district. Her husband Aaron is a mechanic for a local farm equipment dealer. Lynn's fiancé, Brandon, farms with his family near Brook, Indiana, about 145 km, or 90 miles, northeast of the Rohrscheib farm, though he does help Lynn and her family when he can. Lynn and Brandon have a 7-month-old son, Brock, the first member of the next generation with the potential to farm.
"Brock is already excited about tractors and semis, and we look forward to learning if he shares our passion for farming," Lynn says.
The Rohrscheibs also own a custom agricultural input supply business that sells seed, chemicals and fertilizer to other farms. This business also does custom application work, applying inputs for other farmers to an average of 12,140 hectares, or 30,000 acres, each year. They employ a crew of 15 on the farm and for the custom input supply and application business.
As a new mother, Lynn's role on the farm has shifted a bit.
"While I am still involved in the day-to-day work and decisions about what the crew is doing, inputs, where we are working, bookwork and more, I am spending significantly less time in the tractor," she says.
She continues to manage much of the data and information they gather and use to make those daily decisions.
"We rely on innovation in technology, equipment, inputs and more in everything we do," Lynn explains. "For example, I constantly use an online platform on the computer and all our mobile devices to manage what is happening in all our fields. All our equipment uses technology to monitor what we put in the ground and how we care for the crops. And apps for mobile devices help us keep track of what is getting done."
She adds that variable rate technology allows them to apply inputs like fertilizer just where it is needed — both in their fields and when doing custom input application for other farmers.
"Using just what we need makes us more sustainable," Lynn continues. "Innovations provide information so we see in real time what is happening with our equipment. If a row on a planter stops working correctly, the tractor driver can see that right away in the cab and fix the problem."
On-farm technology has made it easier to manage the many types of soybeans and corn they grow. They raise non-GMO soybeans, white and yellow food-grade corn and commodity corn.
"We value biotechnology and the benefits it provides for farmers, and we use it, especially in corn," Lynn says. "However, the premiums available for non-GMO crops and our ability to deliver to a variety of customers make them a good fit for our farm."
Some of their soybeans are exported in containers to Asian markets. The rest of their soybeans become meal for poultry feed in the southeastern U.S. and oil for salad dressing and similar products. They raise white corn for tortillas, chips and other food. Yellow food-grade corn goes to other products, including cereal. Their conventional, GM corn is exported via the Mississippi River system.
Lynn's passion for agriculture and understanding markets — and her tendency to shadow her father — led her to get involved in the industry.
"My dad served on the state soybean checkoff board when I was growing up, so I watched him get involved in the industry," she says. "When I fully joined the farm, I wanted to learn how our soy checkoff dollars are used."
She served as a director for the Illinois Soybean Association, and she now serves on the United Soybean Board. Her industry service showed her the importance of innovation in the soybean industry.
"Our investments in innovation for new soybean markets and uses have helped farmers stay profitable while benefiting our customers with high-quality soy," Lynn explains.
She has visited customers around the world, but one moment stands out: "We were visiting a small soy milk producer in Taiwan who explained that he bought a specific soybean variety," she remembers. "That variety was one we grew, and I realized he could use the soybeans we produce. Then, we saw a shipment of U.S. soybeans come in that included that variety. I realized how small the world is, and the importance of what we do for our customers."
Lynn will share regular updates from her farm on USSOY.org throughout 2022. She will explain how investments in innovation help her family produce high-quality, sustainable soybeans and corn. Lynn and Rohrscheib Farms can also be found on Facebook, Instagram, LinkedIn and Twitter.
Ag communicator Laura Temple tells complex stories from the agriculture industry.
View All Articles in North America
Ground Work 2022: Continuously Improving Sustainability of Michigan Soybeans
Ground Work 2022: Missouri Soybean Harvest Better Than Expected
Neal Bredehoeft
Ground Work 2022: Finishing a Successful Indiana Harvest
Mike Koehne


text:
//
// Copyright 2012 Autodesk, Inc.  All rights reserved.
//
// Use of this software is subject to the terms of the Autodesk license
// agreement provided at the time of installation or download, or which
// otherwise accompanies this software in either electronic or hard copy form.
//
//

#pragma once

#include "RenderItemHandleArray.h"
#include "GenerateMeshRenderItemsContext.h"
#include "UpdateNodeContext.h"

#include "../BaseInterface.h"

/** Interface id of IMeshDisplay2.
*/
#define IMesh_DISPLAY2_INTERFACE_ID Interface_ID(0x177b2945, 0x776a1f62)

namespace MaxSDK { namespace Graphics {

class IMeshDisplay2Impl;

/** Use this interface to query the per node related render items from a mesh or mnesh.
	One can directly query this interface from a mesh or mnmesh object.
*/
class IMeshDisplay2 : public BaseInterface
{
public:
	GraphicsDriverAPI IMeshDisplay2();
	GraphicsDriverAPI virtual ~IMeshDisplay2();

	virtual void PrepareDisplay(const GenerateMeshRenderItemsContext& generateRenderItemsContext) = 0;

	/** This function is usually called by IObjectDisplay2::UpdatePerNodeItems() to
		get accurate render items specified by generateRenderItemsContext to match
		the accurate per-node requirements for the current node's specific render items

		\param generateRenderItemContext contains all the information that is used to generate all
				the required render items.
		\param nodeContext which contains all infos required for adding per node
				relevant render items.
		\param targetRenderItemContainer the target render item container.
		\note DO NOT modify any GeometryRenderItemHandle which are returned in
				targetRenderItemContainer. We cache all GeometryRenderItemHandle
				internally which could be shared by other render nodes. So modify
				them directly may cause unwanted behaviors. If you do have a request
				to modify any GeometryRenderItemHandle, please first manually clone
				them by using another GeometryRenderItemHandle or a RenderItemHandleDecorator.
		\return true if successful or false otherwise
	*/
	virtual bool GetRenderItems(
		const GenerateMeshRenderItemsContext& generateRenderItemsContext,
		UpdateNodeContext& nodeContext,
		IRenderItemContainer& targetRenderItemContainer) = 0;

	// from BaseInterface
	GraphicsDriverAPI virtual Interface_ID GetID();

	GraphicsDriverAPI virtual BaseInterface* GetInterface(Interface_ID id);

private:
	IMeshDisplay2Impl* mpImpl;
};

} } // end namespace

redpajama_set_name: RedPajamaGithub