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Celebrate Cytochemistry
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Cytochemical Methods
Affinity Cytochemistry
EM Immunogold
Dual Immunoperoxidase Labeling for proteins
Smooth endoplasmic reticulum
The Golgi Complex
Lysosomes and Peroxisomes
Membrane Specializations
Membrane Architecture
Membrane Studies
Cilia, flagella, and centrioles
The Cell Nucleus Organization
The Nucleolus
Ribosome Functions on Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Mitochondrial Structure-Function Correlation
Mitochondrial Replication and Genetics
How do proteins enter mitochondria?
Cytoskeletal system
Intermediate Filaments
Actin Cytoskeleton
Medical School Study Guides
Receptor Mediated Endocytosis—Internalization steps
Receptor Mediated Endocytosis- recycling and degradation.
Epithelia
Endocrine System I Neuroendocrine, Pituitary, Thyroid, Parathyroid.pdf
Endocrine_I_Laboratory_Exercises.pdf
Endocrine system II Adrenal, pancreas, pineal, CV, adipocyte.pdf
Practice Practical Exams
Mitosis-Meiosis
Gwen V Childs Academic Program
GH cells as co-gonadotropes
EGF Regulation of Corticotropes
Gonadotrope Cytochemistry and Cytophysiology
Leptin Signaling Pathways
Pituitary gonadotropes are immunolabeled fluorescent green for LH (see above banner) and nuclei stain blue with DAPI. However, in the above view, they are dual labeled for Cre-recombinase with dylight 594 (red) in the nuclei and cytoplasm. This makes the nuclei purple and the cytoplasm yellow.
5 nM Gold markers detect LH in the Golgi complex and in a secretory granule.
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If the inner membrane is so impermeable, how do proteins enter?
The outer membrane of the mitochondria contains the protein "porin". This forms an aqueous channel through which proteins up to 10,000 daltons can pass and go into the intermembrane space. Indeed, the small molecules actually equilibrate between the outer membrane and the cytosol. However, most proteins cannot get into the matrix unless they pass through the inner membrane. This membrane contains cardiolipin which renders it virtually impermeable. This requires transport mechanisms across the membrane that are more organized and regulated. A very simple view of the process is diagrammed in this cartoon.
This figure is taken from Alberts et al, Molecular Biology of the Cell, Garland Publishing, N.Y. 1994, Third Edition
Mitochondrial import signals.
Transport across the mitochondrial membranes requires the concerted action of a number of translocation machineries. The machinery in the outer membrane is called the Tom complex (Translocator outer membrane) and that for the inner membrane is called the Tim complex (Translocator Inner Membrane). Proteins that have to go all the way to the matrix have an NH2 cleavable signal sequence (see the above cartoon).
Most proteins must be uncoiled or stretched out to go through the translocators. This involves ATP binding and is monitored and stabilized by a chaperone proteins, including hsp70. Thus, before the protein can go through Tom complex, it must become "translocation competent".
Transport through the outer membrane: characteristics of Tom complex.
Not surprisingly, the TOM complex will include import receptors that initially recognize the signal peptide or a signal sequence (these include Tom20, Tom22, and Tom70). Different proteins use different receptors. In the above cartoon, the receptor is represented as a blue oval in which the signal peptide is inserted. The receptors then bring the protein to the region containing the translocator proteins. This is actually a complex of proteins.
It is called the General Import Pore (GIP) and it facilitates the translocation of the presequence of the protein across the outer membrane. (the GIP is made of Tom40, Tom5, Tom 6, and Tom7). Tom40 appears to be the core element of the pore and forms oligomers. It traverses the membrane as a series of 14 anti-parallel beta strands which form a beta barrel. It also interacts with polypeptide chains passing through the pore. All of the other Tom components in GIP are anchored to the outer membrane by helical transmembrane segments (hydrophobic anchors).
Recent study of Tom40: Rapaport, D and Neupert W, Biogenesis of Tom40, Core Component of the TOM complex of mitochondria. J Cell Biol 146 321-332, 1999. Study looked at how Tom40 entered outer membrane and became a part of the GIP. The study reported that:
First, as with many mitochondrial proteins, Tom40 requires cytosolic chaperones to prepare it for entry. In the case of this protein, becoming "translocation competent" requires ATP and a partially folded state (the latter is mediated by the cytosolic chaperone (hsp70).
Second, when it is "competent", it interacts with the surface receptor, Tom20. There is no cleavable signal peptide however, the experiments showing the requirement for partial folding suggests targeting information is found in discontinuous sites brought together in the folded domain.
Final insertion is into preexisting Tom complexes. This requires an intact N terminus.
Dimerization occurs after entry into the membrane.
Characteristics of Tim Complexes
Mitochondrial proteins destined for the matrix often have a cleavable signal peptide on the protein which must be recognized before it will be admitted through the mitochondrial translocator. These proteins with "amino terminal signals" (your text), or "preproteins" or "presequences" (current literature) usually interact with Tom20 first. Then, they have to get through the outer membrane. To do that, they are transferred to the GIP complex: First, they interact with Tom22 and Tom5 which ushers them to the pore formed by Tom40. They then enter the matrix using the pore complex made of Tim23 and Tim17 which are in the inner membrane. Also, very important, their entry is dependent on membrane potential. This is set up by the electron transport complexes. Recall that hydrogen ions are being pumped into the intermembrane space creating a charge gradient that is more negative on the matrix site. This membrane potential actually helps pulls the protein into the Tim23-Tim17 channels. The protein then enters the matrix where the cleavable preprotein is clipped off by a protease, MPP. mt-hsp 70 in the matrix works with Tim44 to complete the full transfer to the matrix. mthsp70 and Tim 44 actually "pull" the protein into the matrix by a process that requires ATP. It also requires the membrane potential set up by the electron transport chain.
Some mitochondrial proteins destined for the inner membrane have a cleavable presequence followed by one or more hydrophobic membrane-spanning segments that function as stop-transfer sequences in the IM or, serve to insert the polypeptide into the IM after it gets in the matrix. These are like the Type I membrane proteins described in the unit on the rough endoplasmic reticulum.
However, other proteins do not have a cleavable targeting signal (Types II and III). Mitochondrial proteins that have an internal signal sequence (examples include a number of proteins in the inner membrane) generally interact with Tom70 as the receptor. Then, after they transit the outer membrane via the GIP complex, they enter the special Tim pathway. This may involve interactions with small Tim's of the intermembrane space and Tim22-Tim54 of the inner membrane itself.
Those proteins that do not have a cleavable targeting signal sequence often have signals with the following characteristics: They are often a stretch of positively charged amino acids (sometimes adjacent to a membrane spanning hydrophobic region). Sometimes these form loops that face the matrix. Recall the "positive inside rule" has positively charged amino acids concentrated at the cytosolic side for proteins being inserted into the rough endoplasmic reticulum. These mitochondrial proteins tend to follow this rule, only the matrix becomes the site where the positive charges are most numerous.
Examples from the literature:
Davis, AJ, Ryan, KR, and Jensen, RE Tim23p contains separate and distinct signals for targeting to mitochondria and insertion in to the inner membrane. Molecular Biology of the Cell 9: 2577-2593 (1999).
Tim23 is one of the inner membrane translocator proteins. It does not have an amino-terminal presequence. Targeting information is found within the mature protein.
Tim23 has 4 transmembrane segments and two positively charged loops facing the matrix. What is needed for signalling an import?
Replaced positively charged amino acids in one or both loops with alanine residues.
At least one of these loops is required for insertion into the inner membrane.
The signal for targeting to mitochondria is found in at least two of the hydrophobic transmembrane segments.
Kurz, M, Martin, H, Rassow J, Pfanner, N, and Ryan, MT. Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway. Molecular Biology of the Cell 10: 2461-2474 (1999). Compared the route and binding of three Tim proteins
Tim54 carries a amino terminal, noncleaved translocation sequence that is positively charged. However, it prefers to use Tom70 as its receptor instead of Tom20. After moving through the GIP, it uses its positively charged amino terminal sequence to enter the matrix. It required chaperones and ATP to get to the matrix.
Tim22 is a hydrophobic protein that uses Tom20 for targeting to the OM. Then it follows the Tim route for carrier proteins, like Tim23. It does not require hsp70 or ATP for entry.
Small Tims are normally found in the intermembrane space and are not membrane proteins. They used Tom20 for their receptor and transfer to the GIP complex. However, when Tom20 was destroyed by trypsin, leaving only Tom5, the small Tims were able to enter.
The above cartoon from your text shows more ways that proteins can be inserted into inner and outer membranes, once they are recognized by the receptors. As shown by proteins in the literature examples above, the mitochondria uses both positively charged signals as well as membrane spanning hydrophobic sequences to translocate and then reach their final destination. As in the above examples, there can be multiple signal and insertion sites. However, the distribution of the charged amino acids helps orient the protein so that the positive charges are in the matrix. This is how the cytochromes in the respiratory chain or the elementary particles are inserted by mitochondrial actions.
The following figure is from another text by Lodish et al, Molecular Cell Biology. It shows the entire sequence of events required to take a protein into the matrix.
Step 1: Protein unfolds as it binds to hsp70 chaperone. Red positive area indicates targeting sequence. Chaperone binding is ATP dependent.
Step 2: Targeting sequence binds to receptor (usually Tom20)
Step 3. Receptor ushers protein to site of translocator. Other Tom proteins involved, but Tom40 is the core of the translocator channel.
Step 4: Protein is translocated stimulated by the membrane potential. Electron transport complexes on inner membrane have pumped H+ across to the intermembrane space, leaving the matrix more negative. This attracts the protein (the signal is positively charged). Protein moves through Tim translocators. Tim 44 and hsp70 in the matrix continue to guide and pull the protein through the pore. An ATP requiring process.
Step 5. another chaperone (called a chaperonin), hsp60 causes the folding of the the protein into its tertiary sequence. Also an ATP requiring process.
Step 6. Presequence is cleaved in the matrix.
What happens if an import protein is defective?
Studies of yeast have helped us learn about the receptor and translocation machinery contains a complex of proteins that work together to allow entry. In yeast, these have been named the MOMX.... series, where the number designates the protein number. An important protein in the recognition of the signal peptide and its binding to the receptor is called "MOM19". MOM 19 works with MOM 72 to recognize and bind the proteins. Then MOM22 helps the protein to pass from the receptor binding site to the insertion point at the outer membrane. The importance of MOM19 can be proved by adding antibodies to MOM19 and blocking import.
In a recent paper by Harkness et al (J Cell Biology 124: 637-648, 1995), they created mutant yeast cells that included a defective gene for MOM19.
They also included a drug resistant marker so they could selectively grow cells with the mutant gene (in the presence of the drug, p=fluorophenyl alanine, or fpa). So, the longer the cells grow in the drug, the more drug-resistant mutant cells will be found. The above electron micrographs are from their paper (cited above). They show the result of the absent MOM19 protein. What is absent in the cells grown for 16 or 32 h in the drug?
When they did the assays for the proteins, what proteins were actually missing? Tests showed that there was a dramatic decrease in most of the respiratory chain (electron transport chain) including cytochromes a/a3, and b. However, cytochrome C was unaffected. This suggests that another protein must control its import.
Gwen Childs, Ph.D.,FAAA
Department of Neurobiology and Developmental Sciences
For questions, contact this email address:
http://www.cytochemistry.net
© Gwen Childs Jones, Ph.D. 1995


When you apply for finance with someone, for example a joint credit card or mortgage, a link is automatically created on your credit report. This is known as a 'financial associate'. Once you are linked with this person then it is possible that their credit report will also be taken into consideration for any future credit application you may make.
Before you enter into financial association with anyone, it's vital to be open and honest with each other about your financial commitments and credit histories and check your credit scores.
When do you become financially linked?
It's when you have a joint financial agreement with someone - for example, if you share a joint account or have made a joint application for credit. This will result in their being listed as a financial associate on your credit report.
Just because you marry someone, it doesn't automatically make you financially linked with them – this only happens if you apply for a joint credit account together.
How do I get a financial link removed?
If you wish to remove a financial associate from your credit report, then there are certain steps you can take.
A financial association cannot be removed while joint accounts are open. They will need to be closed or transferred to an individual account. Once done, you can ask credit reference agencies to place a notice of disassociation on your credit report, which should prevent your former associate's actions from affecting your credit report and score in the future.
If you get a divorce or break up with someone, you're still financially linked to them. Until all joint accounts are closed and the financial association removed, their actions – such as taking out new finance – could impact your credit rating too.


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Developing a Corporate Culture that Inspires Creativity with Redpepper
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Eric V. Holtzclaw is a serial entrepreneur who has founded multiple startup companies, including one of the first profitable internet enterprises. He is the author of "Laddering: Unlocking the Potential of Consumer Behavior" and his weekday radio show, The Eric Holtzclaw Show, shines a spotlight on entrepreneurs' individual business journeys and successes.
Economical and Environmental Transportation with Sean FloodSeptember 24, 2018
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Keep On Keeping On: Finding Your Truth with Entrepreneur/Investor, Cade JoinerMarch 12, 2018
Eric V. Holtzclaw is a serial entrepreneur with 20+ years of experience and a founding member of multiple successful start-up companies, including one of the first profitable Internet enterprises and a company that appeared on the Inc. 5000 list three years in a row.
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Surface Travel Awards 2018
Surface Travel Awards
We are pleased to announce the second annual Surface Travel Awards, our tribute to the most thoughtfully conceived spaces and products at the intersection of travel and design.
Deadline: April 13, 2018
At Surface, we explore the world through the lens of design. Great design has long been—and continues to be—a catalyst for creative journeys. These are the Surface Travel Awards: our annual tribute to the world's best, most thoughtfully conceived spaces and products, including hotels, restaurants, airports, and luggage. Celebrating the intersection of travel and design, the Surface Travel Awards recognize and honor architects, designers, and other innovators who inspire, empower, and connect us as we traverse the world. The program aims to raise global awareness of the crucial benefit of design as a means to improve our lives. Held in conjunction with the release of Surface's Travel Issue, the awards ceremony will take place in July 2018.
For all questions related to the Surface Travel Awards, please contact travelawards@surfacemedia.com
Decided by a distinguished panel of jurors, the Travel Awards will honor hotels, restaurants, public spaces, transportation, travel products, and more—as well as the architects, designers, and other innovators who inspire, empower, and connect us as we traverse the world.
Small hotel — Domestic
Overall design and architecture for hotels, inns, B&Bs and similar enterprises in the United States with 100 rooms or fewer. Major refurbishments will be considered.
Small hotel — International
Overall design and architecture for hotels, inns, B&Bs and similar enterprises outside the United States with 100 rooms or fewer. Major refurbishments will be considered.
Large hotel — Domestic
Overall design and architecture for hotels, inns, B&Bs and similar enterprises in the United States with more than 100 rooms. Major refurbishments will be considered.
Large hotel — International
Overall design and architecture for hotels, inns, B&Bs and similar enterprises outside the United States with more than 100 rooms. Major refurbishments will be considered.
Resort — Domestic
Overall design and architecture for self-contained resorts within the United States, offering a broader range of activities and facilities than hotels. Major refurbishments will be considered.
Resort — International
Overall design and architecture for self-contained resorts outside the United States, offering a broader range of activities and facilities than hotels. Major refurbishments will be considered.
Micro hotel/hostel
Overall design and architecture for lodging concepts with compact and/or shared guest rooms and a strong emphasis on communal spaces.
Cruise ship or yacht
Overall design and architecture for a commercial cruise ship or yacht available for charter or private booking.
Overall design and architecture for destination spas, day spas, and spas that are part of a hotel, resort, or cruise ship.
Overall design and architecture for a commercial space devoted to retail sales.
Restaurant — Full-service
Overall design and architecture for a commercial space devoted to food service with table service. The restaurant may be part of a hotel or resort.
Restaurant — Limited service
Overall design and architecture for a commercial space devoted to food service with limited service, such as cafés, coffee shops, candy and ice cream stores, "fast casual" concepts, bakeries, or take-out operations. The restaurant may be part of a hotel or resort.
Overall design and architecture for a commercial bar, tasting room, or nightlife space, including nightclubs and lounges. The space may be part of a hotel or resort.
Design, conceptualization and overall aesthetic impact of an exhibition (temporary or permanent) installed in a museum, gallery, cultural institution, or similar space. Exhibitions like MoMA's "Rain Room" would fall into this category.
Temporary space or installation
Overall design and architecture for a space designed for temporary use, whether retail, food service, arts/cultural, or other application. These include pop-ups, public art displays (for example, Christo's Floating Piers on Lake Iseo), pavilions at design and art fairs, and the like.
Transportation — Conveyance
Interior design for commercial airline cabins, railway cars, subway cars and the like.
Transportation — Stations & terminals
Interior design and architecture for airports, seaports, train and subway stations and other major transportation hubs.
Transportation — Lounges
Interior design and architecture for passenger lounges in airports, train stations, and similar.
City or destination
Cities, towns, or regions whose embrace of design and built environments have benefited both residents and visitors. The entry must include specific improvements realized during the eligibility period (June 1, 2017 – April 1, 2018). Master plans and unrealized developments will not be considered.
Park or public space
Overall design and architecture (including landscape architecture) for a park, purpose-built district, or other environment intended for public use.
Cultural institution
Design and architecture for museums, performance spaces, libraries, religious structures, monuments, memorials, and similar projects. New extensions or expansions of existing structures will be considered.
Mixed-use space
Design and architecture for developments and "entertainment districts" that combine a variety of functions intended for public use, including retail, culture, recreation, food & beverage, commercial, residential, educational and institutional.
Design and architecture for shared workplaces available for use by business travelers (whether membership-based or open to the public). Note that the award will be given for a specific physical location of a co-working space, rather than a collection or a concept.
Short-term vacation rental
Design and architecture for apartments, houses, villas, and other non-hotel lodging made available to travelers on a short-term basis. The space should be marketed on a platform such as Airbnb, onefinestay, HomeAway, or the like, and must have been introduced to the platform between June 1, 2017 and April 1, 2018.
Design of clothing and/or accessories intended for use by staff of a travel or hospitality provider, including airlines, hotels and resorts, cruise lines, railroads, restaurants and bars.
Luggage or travel bag
Overall design for products designed for travelers to carry personal belongings, whether intended as checked, carry-on, or hand baggage.
Travel product or gadgets
Overall design for electronic or other products intended for use by travelers. This category includes cameras, watches, phones, converters, outdoor gear, etc.
Overall design and functionality for headphones, earbuds, or similar devices intended to be used by travelers.
Instagram account — Brand
Overall curation and aesthetic for an Instagram account held by a travel-related brand (hotel, cruise line, destination, airline, etc.). The entry should be for posts dated June 1, 2017 – April 1, 2018, even if the account itself was established earlier.
Instagram account — Individual
Overall curation and aesthetic for a travel- and design-focused Instagram account held by an influencer or other individual. The entry should be for posts dated June 1, 2017 – April 1, 2018, even if the account itself was established earlier.
Open to any travel-related building, interior space, vehicle, system, or product that optimizes its impact on the environment and/or the local community and economy. Jurors will consider the context and impact of the project's sustainability elements as well as the overall design.
Adaptive Reuse Award
Overall design and architecture that reuses an existing site or structure for a travel-related purpose (hotel, restaurant, cultural, retail, transportation, etc.) that is substantially different from that of its original incarnation. (Old hotels that are adapted into new hotels do not belong in this category.)
Travel Designer of the Year
The Surface Travel + Design Award for Designer of the Year will be bestowed on the firm or individual who has had the most profound impact on the travel industry in 2017.
Surface Travel Awards Hall of Fame
Each year, the Surface Travel Awards will nominate five individuals that have made lasting, impactful contributions to the advancement of design in the universe of travel. The final inductee will be decided by a public vote. Nominations will NOT be accepted for the individual Hall of Fame award.
In addition, each year the Surface Travel Awards will accept nominations for specific travel experiences that deserve a place in the Hall of Fame. These will be open to places and experiences that have been open for at least five years (2012 or earlier) and that still exist. Nominations for these awards will be accepted through the normal Surface Travel Awards submissions process; the final inductees will be decided by a public vote.
Hall of Fame — Individual
Awarded to an iconic figure in the world of design whose impact continues to shape the industry on a large scale. This category is not open for submissions.
Hall of Fame — Hotel Swimming Pool
Awarded to a swimming pool that is attached to a hotel or resort, displays the highest aesthetic excellence, and has had a last impact on hospitality design.
Hall of Fame — Freestanding Bar or Lounge
Awarded to a bar, lounge, nightclub or similar venue that displays the highest aesthetic excellence, and has had a last impact on hospitality design. Note that nominees may be attached to a restaurant, but venues attached to a hotel or resort are NOT eligible.
Jury Jury
Anda Andrei
Creative director, interior designer, and president of Anda Andrei Design
Fashion and accessories designer
Daniel Humm
Chef and co-owner of Make It Nice (Eleven Madison Park, The NoMad in New York and Los Angeles, and the fast casual restaurant Made Nice)
José Parlá
Michel Rojkind
Founder and design principal, Rojkind Arquitectos
Graphic designer, painter, and principal of Pentagram
Art collector and cofounder of 21c Museum Hotels
Submissions will be accepted on surfacemag.com during the entry period (February 8—April 13, 2018). Projects in any of the official categories that were completed between June 1, 2017, and April 1, 2018, are eligible (except for Hall of Fame categories). In May 2018, a jury of esteemed architects, designers, artists, chefs, and others will assemble to judge the awards, with the finalists presented at a gala in New York City in July 2018.
The entry must pertain to one of the official categories.
The entry must have been completed between June 1, 2017, and April 1, 2018 (except for Hall of Fame categories).
Entry fee: $295 per initial submission and $195 for additional entries.
All entries must be submitted by the deadline of April 13, 2018.
A single project may be entered into multiple categories (for example, a hotel may enter under the Hotel category and the Adaptive Reuse category, or may submit its restaurant in the Restaurant category). A separate fee will be charged for each entry.
Each submission must be accompanied by a Design Brief (500 words maximum) that explains the project in detail, including the intent, approach and results. Be sure to make a strong case: The judges will use the Design Brief to determine whether the entry deserves recognition.
Each submission must be accompanied by 5-10 photographs, videos, renderings, plans, or other visual materials that will help the judges make their decision. Files should be high-quality and publication-ready and should be sent in PDF format. Maximum file size: 2MB each or 20MB total.
The Awards are global and open to anyone, but submissions must be written in English.
Partners Partners
The Surface Travel Awards is made possible by the sponsors and partners who support our mission to recognize the innovators, spaces, and places inspiring us to travel the world. Special thanks to Cosentino for our custom-crafted awards.
Interested in joining us? Please email Partnerships@surfacemedia.com
The entry period for 2018 has ended. Please check back in June to see this year's winners.


The importance of sleep needs no introduction. You can make do in any position when it comes to forty winks. But when it comes to a question of a good night's sleep, you need the right bed. It is necessary to understand that a bed is typically meant to last really long – very few individuals plan to change their beds frequently. It is therefore necessary to choose the best bed and make no compromises considering that the benefits are expected to outweigh all other cost considerations. Here are four points that you need to bear in mind when purchasing your new bed.
When you intend to go in for a bed, do not make the mistake of only changing the bed, while retaining the old mattress. This will prove to be a wrong choice. While it is true that you may have been comfortable with the old mattress, it will certainly not be the same when you put the old mattress on a new bed. For instance, the coil spring tension may differ, or the size may vary, and this will impact the quality of your sleep. And in terms of cost, your old mattress is certainly not meant to last for ever, you will have to change it at some point of time. It would be prudent to change it with the bed.
Depending on the space requirements in your home, it may be necessary for you to ensure that your bed offers additional storage space. Innovative designs from reputed dealers like The Bed WareHouse Direct will help to make the bed more convenient. For instance a hollow bed post can be a good place to store objects. You could actually use the head post to keep books that you may love to read at bedtime. You could also store emergency medication that you may need to access in the middle of the night without having to rise from the bed or turn on the lights. Common storage options in popular models of beds include storage space below the frames that can be easily accessed.
In the event that you suffer from a back problem that mandates the need for a bed that gives you an adjustable surface, then you need to identify one that meets your special needs. Individuals who shop for beds that are to be used by elders who need assistance in sitting up or who spend more time in bed, will find it beneficial to choose adjustable beds. This will give greater comfort to the occupant and dispense with the need for assistance in helping the individual out of bed.
The bed that you choose should be matched with the mattress that is preferred. This is precisely why it is essential to pick your bed and your mattress at the same time. You will be in a better position to check out the combined effect of the mattress and the bed and enjoy deep slumber always.


We are almost there. I am not sure which day I love most, today or tomorrow. As I think about it I choose… BOTH!!!
Tonight we get to imagine once again sitting around a campfire, with the sheep all bedded down. They are making occasional sheep sounds; like baaah, and buurrr. The shepherds are telling jokes, perhaps aiming them at the occupying Roman Army, or the corrupt and crazy leader in Jerusalem (we get it). It was just another night, nothing special when WOW angels and light!!! As Eugene Peterson says in his Biblical paraphrase, "And the shepherds let loose"! Today is not just a time for bucolic sweetness, but a time to say "Wow, Whoopee, Oh My, How can this be"?
Joy—is what happens when we are filled with gratitude. Luke is very clear about ONE thing: this Gospel is for everyone, all, the whole wide world. Jesus did not come for the church. He came for the world. The reality might be, that it is the nature of God to love us, not because of what we do, but who we are. This is the Good News (Gospel)!!!
LOVE—In Luke's telling of this story this news comes first to those who live and work out on the edges. Love is not just for a select few, the privileged, or educated. Jesus does not belong to a religion, or a political party. His message: that God loves the whole world, and that the Kingdom of God is here and now. This story begins with an 'unwed mother' (Mary), her support team of 1 (Joseph), and some guys who smell like sheep 🐑.
My two years of driving for LYFT have been a gift. It has put me on the streets, where I get to meet the World that God loves.
Thanks for this one, Mark. Keep them coming, my friend.


Roll-Lift USA transported the first 67 meter windmill blades to arrive in the United States. In order to transport these Vestas blades engineered modifications were made to our 48 meter Nooteboom trailers. These blades were transported from shipside to place of rest at Industrial Terminal, eventually they will be transported to rail at Greensport Terminal for shipment to Pueblo Colorado.


All Exclusive Transportation Services Inc. operates with a courteous and efficient packing and moving crew. We have been in business for over 30 years. Our licensed teams assist customers in New York, Northern New Jersey, and Long Island. We are the proud owners of an A+ Better Business Bureau rating. We offer competitive rates and free on-site estimates.
We are available during regular business hours on weekdays, or we are happy to make accommodations for you if you need us to come over the weekend. Please book your appointment and advance.


Remember when The Walking Dead's Sunday night stats were capable of crushing everything in their blood-speckled path? Those days are long gone, and may never return. The extended, game-changing Season 9 premiere, the first under new showrunner Angela Kang, was watched by just 6.1 million viewers. That's the smallest premiere audience since Season 1 debuted.
Let's break down that number a little more to see just how unfortunate things are for The Walking Dead and AMC. The total of 6.1 million viewers is a 47% drop-off from the 11.44 million that watched Season 8's premiere. And that was already the least-watched opener since Season 3 kicked off. Looking back one more year, the Season 7 premiere that killed off Glenn and Abraham had an audience that was 2.8 times bigger than this latest debut.
The comparisons get even worse when the numbers are pared down to just the key demographic ratings. The Walking Dead earned a 2.5 demo rating, with 3.2 million viewers hitting within the 18-49 age range. Those would be ridiculously good numbers for many other shows, but it's actually the lowest-rated Dead premiere ever. It's half of Season 8's 5.0 demo rating, and is even lower than the very first ep "Days Gone By," which landed a 2.7 rating.
When compared to the Season 8 finale, this latest premiere clearly did not carry over as much of the audience as many would have hoped. It was down 23% within the general viewership, and down 27% in the key demo.
To be fair, there are a couple of non-panicky reasons why The Walking Dead's numbers were so low. For one, it's that time of year when Sunday Night Football does everything it can to sap viewers from other primetime programming.
As well, AMC tried out a new tactic with The Walking Dead's Season 9 premiere by releasing it online early. Not for everyone, but just for those who subscribed to the standalone AMC Premiere streaming service over the weekend. According to Deadline, there was a huge surge in signups geared to the special promotion that may have led to the biggest day of new recruits yet. AMC has not released any specific data on that front, though, so it's unofficial for now.
There was certainly much contention going into Season 9 of The Walking Dead, which replaced former showrunner Scott Gimple with writer Angela Kang. The season started off with a time jump into a slightly more civilized society, with viewers already knowing that Andrew Lincoln will be leaving the show soon. Star Lauren Cohan is also set to take a hiatus from her portrayal of Maggie Greene.
It's possible that the viewership totals and the ratings will grow with Episode 2. However, this past weekend sent off some warning bells in fans heads about how much the show's popularity truly is waning. Are you guys still watching?
The Walking Dead airs Sunday nights on AMC at 9:00 p.m. ET. To see what kinds of entertaining will rise up when the zombies fall, head to our fall TV premiere schedule.


text:
Q: How do you sort an alpha-numeric list in excel? I'm  trying to sort a list of documents, but I'm having an issue with the documents that have a letter as a suffix.
Whenever we amend a document we add a letter to the end of the number, but when I sort by number in excel it sorts like this:
1
2
3
10
11
1606
1603D
1605B
1606A
1606C
1610A
1623A
20A
220B
390A
399A
402A
415A
450A
488A
557B

How can I make it sort in order of document number and amendment?
Like so:
1
2
3
10
11
1603D
1605B
1606
1606A
1606C
1610A
1623A
20A
220B
390A
399A
402A
415A
450A
488A
557B

A: As long as you have a mix of text and number, you won't be able to use Excel's built-in sort to achieve the result you describe.
If you append a letter to a number you effectively change the data type from number to text. Text will always be sorted after any number, hence the number 1606 comes before the text 1606A.
You could try to make all values real text, maybe indicate levels by appending digits with dots, like this:
1.
1.0.
1.1.
1.6.0.3.D
1.6.0.5.B
1.6.0.6.
1.6.0.6.A
1.6.0.6.C
1.6.1.0.A
1.6.2.3.A
2.
2.0.A.
2.2.0.B.
3.
3.9.0.A.
3.9.9.A.
4.0.2.A.
4.1.5.A.
4.5.0.A.
4.8.8.A.
5.5.7.B.

But even that does not give you the sort order you describe as the desired result.
Your desired sort order will be hard to achieve even if all values are text, or if you replace the A, B, C with a decimal .1, .2, .3. -- It's really hard to understand why 20 would come after 1623.

A: The solution I found was to add a column, and copy this formula into each cell:
=IF(ISNUMBER(--RIGHT(A2)),A2,LEFT(A2,LEN(A2)-1))

The formula removes the letters from the numbers, you can then sort your sheet using the new column of clean numbers.

redpajama_set_name: RedPajamaStackExchange


Bruno Biais, Christophe Bisière, Matthieu Bouvard et Catherine Casamatta, « The blockchain folk theorem », The Review of Financial Studies, vol. 32, n° 5, mai 2019, p. 1662–1715.
Bruno Biais, Florian Heider et Marie Hoerova, « Risk-sharing or risk-taking? Counterparty-risk, incentives and margins », The Journal of Finance, vol. 71, n° 4, août 2016, p. 1669–1698.
Bruno Biais, Christophe Bisière et Sébastien Pouget, « Equilibrium Discovery and Preopening Mechanisms in an Experimental Market », Management Science, vol. 60, n° 3, mars 2014.
Bruno Biais et Thierry Foucault, « High-Frequency Trading and market quality », Bankers, Markets & Investors, vol. 128, 2014, p. 5–19.
Bruno Biais, Johan Hombert et Pierre-Olivier Weill, « Equilibrium Pricing and Trading Volume under Preference Uncertainty », The Review of Economic Studies, vol. 81, n° 4, 2014, p. 1401–1437.
Bruno Biais, Florian Heider et Marie Hoerova, « Incentive compatible centralised clearing », Financial Stability Review (Banque de France), vol. 161, avril 2013.
Bruno Biais, Florian Heider et Marie Hoerova, « Clearing, counterparty risk and aggregate risk », IMF Economic Review, n° 60, juillet 2012, p. 193–222.
Bruno Biais, Christophe Bisière et Chester Spatt, « Imperfect Competition in Financial Markets: An Empirical Study of Island and Nasdaq », Management Science, vol. 56, n° 12, décembre 2010, p. 2237–2250.
Bruno Biais, Peter Bossaerts et Chester Spatt, « Equilibrium Asset Pricing And Portfolio Choice Under Asymmetric Information », The Review of Financial Studies, vol. 23, n° 4, 2010, p. 1503–1543.
Bruno Biais et Thomas Mariotti, « Credit, Wages, and Bankruptcy Laws », Journal of the European Economic Association, vol. 7, n° 5, septembre 2009, p. 939–973.
Jean-Paul Azam, Robert H. Bates et Bruno Biais, « Political Predation and Economic Development », Economics & Politics, vol. 21, n° 2, juillet 2009, p. 255–277.
Bruno Biais et Martin Weber, « Hindsight Bias, Risk Perception and Investment Performance », Management Science, vol. 55, n° 6, juin 2009, p. 1018–1029.
Bruno Biais et Enrico Perotti, « Entrepreneurs and New Ideas », The RAND Journal of Economics, vol. 39, n° 4, Winter 2008, p. 1105–1125.
Bruno Biais, « Les scandales financiers: Aléa moral ou problème éthique? », Esprit, mai 2008.
Bruno Biais, « La Banque Centrale Européenne est-elle trop indépendante? », Esprit, février 2008.


text:
Thomas F. Freeman Collection, no. 85 - TSU Book Fair (1972)
Thomas F. Freeman
Book Fair at TSU
Sign for the Book Fair
Secretary with typewriter
Students shopping the book fair
Thomas F. Freeman Thomas F. Freeman
This home movie captures scenes of the 1972 Book Fair at Texas Southern University.
Dr. Thomas F. Freeman was born in 1919 in Richmond, Virginia, where he also spent his childhood and attended college. Freeman left Virginia temporarily to serve a nine month contract at Houston's Texas Southern University in 1949. Many decades later, Dr. Freeman is still a professor and debate coach at TSU, on campus six days a week, and has helped multiple generations of young Texan African-American students find their voice and rise to new heights of scholarly achievement. A pillar of the Houston community, Dr. Freeman also has ministered at Mt. Horem Baptist Church for more than 65 years, still delivering sermons every Sunday; taught Religious Studies at Rice University for 20 years; helped found Houston's Model Cities program; founded and served as Dean of TSU's Weekend College; was the Founding Dean of TSU's Honors College; and over the course of his teaching career, taught and influenced many prominent African-Americans leaders, including Otis King, Barbara Jordan, and Martin Luther King, Jr.
Dr. Thomas F. Freeman
Dr. Thomas Freeman
Thomas Freeman
Freeman, Thomas
Freeman, Thomas F.
Categories: The Thomas F. Freeman Collection | 1970s | Education | Houston | Harris County


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The French Maid Costume – Time to clean up…in the bedroom.
Available until stock runs out. Limited quantities.
SKU: N/A. Categories: Lingerie, SPECIALS / CLEARANCE.	Tags: costume, dress up, french maid, french maid costume.
This black stretch spandex dress with apron and collar is a sure way to liven up the boudoir….


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TAKING A NEW POST: Blake Naughton is moving from Missouri to Colorado to take a new position: vice president of engagement and Extension at Colorado State University.
Colorado names new Extension vice president
Blake Naughton joins CSU staff from Missouri with a mission to advance engagement and outreach.
The Extension work of any land-grant college is a significant responsibility, and it's evolving every year. Colorado State University announced recently that Blake Naughton has been brought in as vice president for engagement and Extension, and he will take the reins of the renamed Office of Engagement and Extension Dec. 1.
According to the statement announcing his hiring, Naughton will advance CSU's engagement and outreach missions and oversee CSU Extension, CSU Online, the Colorado Water Center and the Office of Community and Economic Development.
Naughton was most recently associate vice chancellor for Extension and engagement at the University of Missouri. In that role he provided day-to-day leadership for Extension, and continuing education programming for agriculture, youth, the economy and health.
CSU President Joyce McConnell noted that the university is committed to serving the state by providing research-based solutions to help Coloradans face complex challenges. "Because of this commitment, we are thrilled to welcome Blake to our team. He is a recognized leader in developing partnerships, improving education and leading the renewal of educational institutions, and he will help us strategically transform how CSU serves Colorado communities," she says.
Naughton will work with the CSU leadership team including Ashley Stokes, who will serve as associate vice president for engagement and Extension and will provide day-to-day leadership of CSU Extension. Stokes joined CSU's College of Veterinary Medicine and Biomedical Sciences in 2015 as the associate dean of veterinary admissions and student services.
CSU's Office of Engagement and Extension has more than 400 employees across all eight CSU colleges and works closely with the office of the Vice President for Research.
More about Naughton
Naughton grew up in Missouri and worked as a policy researcher for Congress, the state of Missouri and a national center focused on higher education. He led educational programs and engagement services on the faculty of Northwest Missouri State University and the University of Pennsylvania before joining the College of Education at the University of Missouri, Columbia.
He holds a bachelor's degree in technology policy from Stanford University; a master's degree in education administration, planning and social policy from Harvard University; and a doctorate in education administration and policy analysis, also from Stanford. Building on his expertise in educational organizations, policy, youth and workforce development, Naughton will also join the faculty of the CSU College of Health and Human Sciences as a professor in the Department of Human Development and Family Studies.
With a mission to provide educational information and programs that safeguard health, increase livelihood, and enhance well-being, the Colorado State University Extension Service serves all of Colorado's 64 counties. In 1914, Congress authorized land-grant universities in every state to feed research-based information to Extension agents in each county.
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text:
Invasion of the Spirit People
Juan Pablo Villalobos's fifth novel adopts a gentle, fable-like tone, approaching the problem of racism from the perspective that any position as idiotic as xenophobia can only be fought with sheer absurdity.
In an unnamed city, occupied by an unnamed world power, an immigrant named Gastón makes his living selling exotic vegetables to eateries around the city. He has a dog called Kitten, who's been diagnosed with terminal cancer, and a good friend called Max, who's in a deep depression after being forced to close his restaurant. Meanwhile, Max's son, Pol, a scientist away on a scientific expedition into the Arctic, can offer little support.
Gastón begins a quest, or rather three: he must search for someone to put his dog to sleep humanely; he must find a space in which to open a new restaurant with Max; and he must look into the truth behind the news being sent back by Pol: that human life may be the by-product of an ancient alien attempt at colonization . . . and those aliens might intend to make a return visit.
Author: Juan Pablo Villalobos
Publisher: And Other Stories


Swift is Apple's new generation of programming languages. Using models from Objective C and Cocoa, Swift is the answer to all the current issues involved in coding with older versions when creating applications for iPhone and iPads. It's conception is largely based in offering more resiliency against errors in code that have arisen when programming with Objective C, hence its playful handle "Objective C without the C". Nuvious is one of the few companies that already excels at Swift, given it hasn't been on the market for very long. So, for a cleaner and more problem free environment for your applications, let our team introduce you to the new world off Apple apps.


text:
Q: P5.js - Using erase() in instance mode returns "p.erase is not a function" I'm currently using p5.js in instance mode and I'm using the following block of code that's called by draw function of a p5 object. The draw function of the p5 object passes the p5 object as p to the below draw function.
//Snippet 1
this.draw = function(p){
    ...

    p.push();
    p.noStroke();
    tempColour = someFunc(); //returns color object
    p.fill(tempColour);
    p.ellipse(p.mouseX, p.mouseY, diameter);
    p.pop();
    ...
}

The above code works as expected without exceptions.
Now as soon as I use the erase() function (see below) the exception TypeError: p.erase is not a function. (In 'p.erase()', 'p.erase' is undefined) is thrown in the console.
//Snippet 2
this.draw = function(p){
    ...

    p.push();

    //newly inserted start
    p.erase();
    p.ellipse(p.mouseX, p.mouseY, diameter);
    p.noErase();
    //newly inserted end

    p.noStroke();
    tempColour = someFunc(); //returns color object
    p.fill(tempColour);
    p.ellipse(p.mouseX, p.mouseY, diameter);
    p.pop();
    ...
}

I have tried to use the erase() function before push() is called but unfortunately the result is the same. I've also checked that I'm not accidentally reassigning/redeclaring erase on the p5 object.
I'm struggling to understand how erase() is any different to fill() or stroke() or any other p5 function and how it might cause the exception.
My goal:
The colours I pass to the ellipse function may contain an alpha value of less than 255. The code in Snippet 1 currently draws the ellipses on top of each other which ultimately results in a fully opaque ellipse (if mouseX and mouseY remain unchanged).
What I'm trying to achieve with Snippet 2 is for the ellipse function to draw the exact colour I'm passing to it without the effect of the increased opacity.
Any help is much appreciated

A: Problem solved, I was using an old version of the p5 library.


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ConTEXT – darmowy edytor tekstu dla twórców oprogramowania.

Może otwierać i edytować pliki teoretycznie dowolnych rozmiarów. Obsługuje linie o długości do 4 kB oraz podświetlanie składni dla C, Delphi/Pascala, asemblera 80x86, Javy, JavaScriptu, Visual Basica, Perla/CGI, HTML, SQL, Pythona, PHP, Tcl/Tk, XML, Fortranu, FoxPro; posiada również swoje zdefiniowane szablony podświetlania składni. Ponadto oferuje podstawowe funkcje związane z wyrażeniami regularnymi. Integracja z kompilatorem jest jego kolejną zaletą. Dostępne są różne wersje językowe. Wbrew informacjom zawartym na stronie internetowej edytora, nie wspiera on kodowania UTF-8.

ConTEXT jest zwycięzcą nagrody Pricelessware w 2006 roku.

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 Witryna programu ConTEXT

Edytory tekstu

redpajama_set_name: RedPajamaWikipedia


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Tieshan () is a town in Zhenghe County, Fujian province, China. , it administers Niubeishan Residential Community () and the following fourteen villages:
Tieshan Village
Nangan Village ()
Donggan Village ()
Litunyang Village ()
Dahong Village ()
Jiangshang Village ()
Zhangtun Village ()
Xiangqian Village ()
Gaolin Village ()
Banyuan Village ()
Fenglin Village ()
Yuanshan Village ()
Daling Village ()
Luojiadi Village ()

References

Township-level divisions of Fujian
Zhenghe County


IMPORTANT: What follows are the materials you need for class. However, you can wait until the first discussion class (lecture) meets to start getting the needed materials, since you will have two weeks after the first discussion class meets to track down the items.
or any other iClicker remote will work.
1) You can buy directly from SIMnet for $90.
You can do this during your second lab meeting. You will need to use a credit card.
2) You can buy from SSU bookstore for $126.
3) You can buy from Northern Light bookstore for $(usually about $9 cheaper then SSU bookstore).
If you buy from either Northern Lights or the SSU bookstore you will end up with a document that contains an area that can be scratched off to reveal an access code. You just need to bring this access code to your second lab meeting.


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Fabiola est un nom propre qui peut désigner :

Prénom
 Fabiola est un prénom féminin.

Sainte chrétienne
 Fabiola est une sainte chrétienne du .

Arts
  est un roman anglais de Nicholas Wiseman, publié en 1854, et adapté en film :
 Fabiola est un film italien réalisé par Enrico Guazzoni, sorti en 1918 ;
 Fabiola est un film italien d'Alessandro Blasetti, sorti en 1949.
 Fabiola est un titre de l'album Tékit izi du groupe antillais Kassav.
Fabiola est le nom d'un groupe de pop-rock belge indépendant.
 2 Fabiola est un groupe de dance belge

Personnalité
 Fabiola de Mora y Aragón (1928-2014) est une reine des Belges, épouse du roi Baudouin (1930-1993).

Astronomie
 (1576) Fabiola est un astéroïde.

Homonymie de titre


With only a couple days to go, it looks like Amazon is sending out emails to those that signed up to inform them of the purchase process for the upcoming NES Classic Edition.
As expected, the NES Classic Edition will be offered in limited quantities on release day. According to the email, Amazon will begin selling the console starting at 2pm PT. Those ordering will not be able to utilize the expedited "1-Click" ordering option and will have to go through the manual purchase process. Although demand will be high, they will make additional announcements whenever the unit comes back in stock.
Amazon is the first retailer to publicly outline its purchase process in the US. The official Nintendo website does also list Best Buy and Target as supported merchants, but neither have offered a pre-order process or outline thus far. Those in the UK that have secured their unit via a pre-order can rest easy, while those in the US might be in for quite the surprise come Friday.
Apr 5, 2019 · Hot!


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Th1rteen R3asons why I couldn't stop watching (non-spoiler review)
2 years ago By Albert Burdge
I'm getting to the age that high school dramas don't really relate to me and my life, but 13RW was recommended by my wife and her friend. Those two ladies have good taste, and I love a good Netflix marathon just like anyone else so I gave it one episode to hook me, unfair to any new show, but after the first episode, I was hooked.
The series, based on the novel by Jay Asher and produced by Selena Gomez is intriguing, to say the least. I had heard the buzz and thought why would I want to watch a show about a girl who commits suicide? Some are saying it glorifies it even. I would hate for that to be the case, if we lost one young person that may be inspired by this show, it would be one too many. I didn't want to judge a show by its thumbnail (remember the Gilmore Girls fiasco?) so I went in with an open mind.
The story is told to us through a series of 7 cassette tapes recorded by Hannah Baker (Katherine Langford) as a sort of suicide note/mystery game that presumably 13 people have to listen to and pass on to the next person in the sequence all the while being watched over by a mysterious Joe Cool type named Tony (Christian Navarro) and being obsessed over by the previous listeners. We jump in on Clay's (Dylan Minnette) turn to listen. Unlike the others listeners, Clay listens very slowly, luckily for us to stretch it out to 13 episodes.
Immediately I get this Veronica Mars vibe without the cheese mixed with the drawn out mystery of Reunion. Events of the past and present play out simultaneously and overlap, which forces audiences to engage their brain, put their phone down and get sucked in. For me, the only problem I had getting into it was the whole high school full of 27-year-old students, but once I accepted it, there was no turning back.
Hannah's mom played perfectly by the amazing Kate Walsh carries the bulk of the grief and gravitas of the situation that is this story. Without which everything wouldn't be as important as it is while watching. The biggest mystery for the first half of the series is how could the one person who seems to have her shit together more than anyone else kill herself? Is everything Hannah is saying on these tapes true? How does everyone fit into the mystery? And just how could something like this happen.
I lost a sister to suicide when she was 16 years old. High school bullshit is real, and what we sometimes forget as adults are the things we think are inconsequential now, were at one time the biggest problem we ever had in our lives up until that time. When we are kids, if we don't get the toy we wanted, or can't stay up late, it's the end of the world. In our teen years, whether a boy likes us or not is the end of the world. In our thirties, marriages ending seems like the end of the world. Are you sensing a pattern yet? It's only for now. When I was bullied in high school, I had a fleeting thought about how to end it. That scared me and luckily for me, I changed my situation, got out of there and had parents that supported me, and knew I was more important than anything else. It does get better. Hang in there, kid.
As a parent, I can't even imagine being in a situation like Hannah's parents are in. How could someone even write about this? And why am I watching it? I have no clue, but I can't stop. The writing and acting are amazing. I recommend this as a must watch. I have to know how it ends, back to Netflix I go.
Update: I finished it. Wow.
<3 Albie 20170519CE
Who is Albie?
Tags: 13, 13 reasons why, non spoiler review 13 reasons why, thirteen reasons why
Previous FMV Gaming- Get your geek on!
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'Glass Onion: A Knives Out Mystery' reveals its layers
By Jared Rasic
There's something that makes me feel a little like I'm chewing sour grapes when I sit down to pick apart a movie that ultimately exists just to wildly entertain people. Like, there's nothing inherently "bad" about the movie or that makes it a lesser exercise in cinema, but ultimately feels like the popcorn we munch while watching it: empty with little to no nutritional value. Which (because the pandemic taught me how to keep myself occupied by creating pointless thought exercises in my head) makes me wonder if a movie has the responsibility to be more than that.
Photo courtesy of Netflix
Daniel Craig, far right, leads another stellar cast in this entertaining whodunit.
A case in point is "Glass Onion: A Knives Out Mystery," a movie that held me in its palms for basically all of its 139-minute runtime, but basically only exists as a delivery device to show an audience how clever writer/director Rian Johnson is at plotting and jokes. The first "Knives Out" felt like a breath of fresh air at the time because we don't get old-fashioned murder mysteries packed with movie stars with massive budgets that often anymore, plus it was a super-fun throwback to films like "Clue," "Murder By Death" and "Murder on the Orient Express."
"Glass Onion" doesn't have the element of surprise working for it this time, but what it does have is a stacked cast, a breathtaking new location and an engaging central whodunnit. With Kathryn Hahn, Kate Hudson, Dave Bautista, Leslie Odom Jr., Madelyn Cline and a scene-stealing Jessica Henwick supporting, and with Edward Norton, Janelle Monae and Daniel Craig front and center, there isn't a weak link in the bunch.
Without giving too much away, the setup is delicious: Norton is a tech billionaire who invites a group of his oldest friends to his private island to participate in a scripted murder mystery. World's greatest detective Benoit Blanc (Daniel Craig, having an absolute blast) also arrives on the island just in time to make use of his skills of deduction to solve a very real crime. Hijinks ensue.
Filmed on the sumptuous island of Spetses in Greece, "Glass Onion" is visually much more exciting than the Boston mansion from the original, but even with such a gorgeous new locale and that excellent cast, not enough time is spent developing the characters to really invest the audience in their fate. Janelle Monae has the juiciest role of the new cast members by far, but even her role feels like it's in service of the mystery, rather than the other way around. The plot should always serve the characters because that's how you find truth in storytelling.
Everything in "Glass Onion" is bigger than in "Knives Out." Even the script is structurally much more daring than the original, though it's constructing a mystery that I didn't find anywhere near as enveloping. Every single line of dialogue and every action taken by a character is in service of a shell game meticulously designed by Rian Johnson, but, just like most shell games, it's primarily designed to trick you and leave you with nothing. Halfway through the film, I started thinking about the suspects from "Knives Out:" Don Johnson, Michael Shannon, Jamie Lee Curtis, Chris Evans, Katherine Langford Jaeden Martell and Ana de Armas were precisely detailed and drawn characters. The suspects in "Glass Onion" are caricatures (aside from maybe Janelle Monae) and by the third act I couldn't care less about how their stories turned out.
Still, this is all picking at a movie that's actually extremely fun to watch as you study Benoit Blanc proving why he's the world's greatest detective. Even though I wasn't as in love with "Glass Onion" as I was with "Knives Out," I would gladly watch Daniel Craig chew up the scenery a dozen more times as Blanc. Maybe that's all the movie set out to do: just entertain me enough to come back next time. Maybe that's enough sometimes, even as I dream for something more.
Dir: Rian Johnson
Regal Old Mill
Releases on Netflix 12/23
Jared Rasic
Film critic and author of food, arts and culture stories for the Source Weekly since 2010.
Read More about Jared Rasic
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