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Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii Advances in Interventional Cardiology/Postępy w Kardiologii Interwencyjnej Anaesthesiology Intensive Therapy Archives of Medical Science Biology of Sport Central European Journal of Immunology Folia Neuropathologica Journal of Contemporary Brachytherapy Pediatric Endocrinology Diabetes and Metabolism Polish Journal of Pathology Prenatal Cardiology Videosurgery and Other Miniinvasive Techniques
Polish Journal of Pathology
Current issue Archive Manuscripts accepted About the journal Supplements Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis
Maciej Foks
Małgorzata Wągrowska-Danilewicz
Marian Danilewicz
Marta Bonczysta
Bogusław Olborski
Olga Stasikowska-Kanicka
Pol J Pathol 2019; 70 (3): 217-222
DOI: https://doi.org/10.5114/pjp.2019.90399
- 09-PJP-01449.pdf [1.71 MB]
Foks M, Wągrowska-Danilewicz M, Danilewicz M, Bonczysta M, Olborski B, Stasikowska-Kanicka O. The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis. Polish Journal of Pathology. 2019;70(3):217-222. doi:10.5114/pjp.2019.90399.
Foks, M., Wągrowska-Danilewicz, M., Danilewicz, M., Bonczysta, M., Olborski, B., & Stasikowska-Kanicka, O. (2019). The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis. Polish Journal of Pathology, 70(3), 217-222. https://doi.org/10.5114/pjp.2019.90399
Foks, Maciej, Małgorzata Wągrowska-Danilewicz, Marian Danilewicz, Marta Bonczysta, Bogusław Olborski, and Olga Stasikowska-Kanicka. 2019. "The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis". Polish Journal of Pathology 70 (3): 217-222. doi:10.5114/pjp.2019.90399.
Foks, M., Wągrowska-Danilewicz, M., Danilewicz, M., Bonczysta, M., Olborski, B., and Stasikowska-Kanicka, O. (2019). The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis. Polish Journal of Pathology, 70(3), pp.217-222. https://doi.org/10.5114/pjp.2019.90399
Foks, Maciej et al. "The number of CD163 positive macrophages is associatedwith more advanced skin melanomas, microvessels density and patient prognosis." Polish Journal of Pathology, vol. 70, no. 3, 2019, pp. 217-222. doi:10.5114/pjp.2019.90399.
Melanoma is the most aggressive and lethal malignant neoplasm of the skin. If diagnosed at late stages, the survival rates are extremely poor [1, 2]. Therefore, new indicators of melanoma progression and predictors of treatment response are urgently needed. Because melanoma has an immune-related pathogenesis, the composition of tumor microenvironment is considered as an important prognostic factor modifying the patient outcome [3].
Literature data documented that tumor-associated macrophages (TAMs) are one of the most prominent bone marrow derived cells recruited into tumor microenvironment. In the skin melanoma, TAMs play crucial role in promoting melanocytes neoplastic transformation, proliferation and invasion [4, 5, 6, 7]. CD68 is a scavenger receptor highly expressed on macrophages and generally accepted as a pan-macrophage marker. Based on the expression of cytokines, receptors, and effector molecules, macrophages are classified into two main subsets – classically activated M1 macrophages with pro-inflammatory function and into alternatively activated M2 macrophages which are closely involved in tumor promoting processes [8, 9, 10, 11]. M1 macrophages are typically recognized by the expression of inducible nitric oxide synthase (iNOS) which is a crucial enzyme in arginine metabolism pathway [12, 13]. Nitric oxide (NO) has cytotoxic effect that contributes to the anti-tumor effect of M1 macrophages. M2 macrophages express the mannose receptors, scavenging receptors CD163 and CD204, high levels of the chemokines CCL17, CCL22 and CCL24, and low levels of IL-12 and have been suggested to contribute to tissue remodelling, angiogenesis, and tumor progression [8, 14]. The literature data suggest that melanoma's microenvironment tends to polarize macrophages toward M2 phenotype which is connected with higher expression of proangiogenic factors such as VEGF. Furthermore, the environment of melanoma was proven to be highly hypoxic which is also a very significant factor for angiogenesis [15, 16].
The aim of our study was to examine the number of TAMs and describe the macrophage subpopulations (M1 and M2) in 46 cases of skin melanoma divided according to Breslow scale from I to IV. Another purpose was to find possible associations between the number of M1 (iNOS positive), M2 macrophages (CD163 positive) cells, microvessels density (MVD) and patients' survival times.
The present retrospective study and experimental methods were approved by the university review board, and have been performed in accordance with the World Medical Association Declaration of Helsinki regarding ethical conduct of research involving human subjects. The study was conducted under the assumption that research findings would be kept anonymous.
We examined 46 formalin-fixed, paraffin-embedded tissue specimens of skin melanomas that were diagnosed in Health Care Institution Diagnostics Consilio in Lodz. Paraffin-embedded tissue sections taken from postoperative material were diagnosed using standard hematoxylin and eosin staining. The histological diagnoses were established according to the WHO classification of skin tumors [17]. All cases were divided into 4 groups according to the Breslow scale (group I: ≤ 1.0 mm, n = 12; group II: 1.01-2 mm, n = 10; group III: 2.01-4.0 mm, n = 10; group IV: > 4,0 mm, n = 14). None of the patients had metastatic disease at the time of diagnosis. The follow-up data including overall survival were received for all studied cases.
Immunohistochemical staining was carried out according to a standard method. 3-µm tissue sections were deparaffinized in xylene and rehydrated through a graded alcohol series. Heating in a microwave oven in a solution of target retrieval solution pH 9.0 (TRS, Dako), for 30 minutes was used for antigen retrieval. Endogenous peroxidase were quenched with 0,3% hydrogen peroxide in methanol for 30 minutes.
The sections were washed with TBS and incubated from 30 to 60 minutes with monoclonal mouse primary antibodies against: CD68 (Dako, Denmark; clone: PG-M1, dilution 1:100), CD163 (Abcam, UK; clone 10D6, ready-to-use), iNOS (Thermo Scientific, USA; PA 1 - 21054, dilution 1:100), CD34 (Dako; clone: QBEnd 10, dilution 1:50). After washing, an adequate EnVision-HRP detection system (Dako, Carpinteria, CA, USA) was used. 3,3'diaminobenzidine was used as the chromogen. After counterstaining with Mayer's hematoxylin, the slides were washed, dehydrated, cleared in xylene and coverslipped. The negative controls involved the same procedure as for positive ones, with the primary antibodies replaced by antibody diluent.
CD68, CD163 and iNOS positive cells were evaluated using a computer image analysis system consisting of a PC equipped with a Pentagram graphic tablet, Indeo Fast card (frame grabber, true-color, real-time), produced by Indeo (Taiwan), and a color TV camera Panasonic (Japan) coupled with a Carl Zeiss microscope (Germany). This system was programmed (MultiScan 18.03 software, produced by Computer Scanning Systems, Poland) to calculate the number of objects (semiautomatic function).
Intratumoral macrophages were counted in the melanoma cell nests and the stroma by the hotspot analysis. The number of CD68, CD163 and iNOS positive cells was estimated by counting all positive cells in 7-10 high power monitor fields (HPF) (0.029 mm² each), marking immunopositive cells (semiautomatic function).
CD34 immunostains were evaluated in the vessels only (not in the individual cells), in the most vascular areas (hot spots) of the tumor. The results were presented as the mean number of CD34 positive vessels with visible lumina per HPF (0.029 mm²).
Differences between groups were tested using unpaired Student's t-test preceded by evaluation of normality and Levene's test. The Mann-Whitney U test was used where appropriate. Correlation coefficients were calculated using Spearman's method. For statistical purposes all cases were divided for those below and above median of the particular parameters (mediana for CD68 was 20, for CD163 was 8, for iNOS was 5, and for CD34 was 7,5). The survival analysis between these groups was performed using Kaplan-Meier method.
For comparison Kaplan-Meier curves the proportional hazard Cox regression test was used. For Kaplan-Meier estimator death was the point-of-interest and the observation period was 80 month. Results were considered statistically significant if p < 0.05.
The quantitative data of the immunoexpression of CD68, CD168, iNOS positive macrophages and CD34 positive vessels (microvessel density – MVD) are presented in Table I.
To detect all TAMs, M1 and M2 type macrophages, tissue sections were stained with CD68, iNOS and CD163 antibodies, respectively. iNOS immunoreactivity was localized in the cytoplasm. Both CD163 and CD68 immunoreactivities were localized mainly in the cytoplasm, and in some cases also on the plasma membrane. Staining patterns were often granular.
In our study, the mean number of CD68 positive cells was increased in successive stages of Breslow scale, but statistically significant difference was observed only between I and IV group. The mean number of CD163 positive cells was significantly increased in group III and IV compared to group I (Fig. 1A, B).
The mean numbers of iNOS positive cells were similar in all studied groups. There were no statistical differences between mean numbers of iNOS positive cells in studied groups. We also found a significantly lower number of microvessels in group I compared to all other studied groups (Fig. 1C, D)
In all cohort of the patients, there were positive significant correlations between the mean number of CD68 and CD163 positive cells and microvessels density (R = 0.47, p < 0.001; R = 0.44, p < 0.002 respectively), whereas the correlation with iNOS was not statistically significant.
Moreover, we observed positive significant correlation between iNOS positive cells and survival times (R = 0.28, p < 0.02), and negative significant correlations between CD163 positive cells and survival times (R = –0.72, p < 0.001), as well as between microvessels density and survival times (R = –0.43, p < 0.003).
However, the Kaplan-Meier survival analysis showed that only accumulation of CD163 positive macrophages were associated inversely with patient's survival time (p < 0.04; Fig. 2).
Tumor microenvironment is a field of constant struggle between malignant tumor cells and patient's immune system. Immune cells, fibroblasts, endothelial cells, pericytes and other cells that are included in microenvironment interact both with each other and with tumor cells [18, 19]. Literature data suggest that tumors may promote or alter different immune cells phenotypes and either suppress the immune system or raise the tolerance for tumor antigens which would lead to tumor progression and metastasis [4, 5, 6, 7]. Tumor-associated macrophages are the major immune component of the melanoma microenvironment.
Literature data showed that TAMs presence is connected with tumor initiation and development, immunosuppression, angiogenesis, invasion and metastasis [20, 21, 22]. Our study showed that the number of CD68 positive TAMs were increased in subsequent stages of melanomas according to Breslow scale, even though statistically significant difference was observed only between I and IV group. In line with our findings, Hillen et al. [23] demonstrated that melanomas in the vertical growth phase contain more CD68 positive TAMs compared with melanomas in the radial growth phase. Recently, Falleni et al. [5] reported that CD68 accumulation was correlated with Breslow depth. All of this findings support the thesis that, TAMs can stimulate tumor invasion.
TAMs can display various functions depending on their polarization state [8, 10]. M1 macrophages are proinflammatory and antigen presenting subtype with cytotoxic NO as the product of their metabolism. They release IL-12, IL-23, TNF-a which, among other effects, stimuli T cells to induce cytotoxic response [9, 11, 14]. Literature data showed that the M1 infiltration is usually associated with immunostimulatory functions and extended patient survival in many human cancer [14, 24]. In our study, the mean number of intratumoral iNOS positive cells was similar in I, II and III group according to the Breslow scale. Only in group IV, the number of iNOS positive cells was slightly higher but with no statistical significance. Our results may suggest that the intratumoral M1 macrophage infiltration is not associated with the skin melanoma depth. In contrary to our results, Falleni et al. [5] reported correlation between a high density of M1 macrophages in melanoma and the Breslow's thickness. The role of M1 macrophages in the tumor microenvironment is not completely clear what is reflected by many conflicting reports concerning the number and localization of M1 positive cells in the microenvironment of malignant melanoma [5, 25, 26, 27]. Differences concerning an association between the number of M1macrophages and Breslow depht in various studies may indicate that the precise role of M1 macrophages and immunological mechanisms taking part in malignant melanoma progression is still not fully understood. Furthermore, we should take into consideration that although iNOS was used in several studies to mark intratumoral M1 macrophages, it is not the best marker to evaluate M1 macrophages, because its expression was not restricted to infiltrating TAMs, but also involved the malignant melanoma cells.
M2 macrophages are known to produce various cytokines, chemokines and enzymes that are connected with tumor progression, angiogenesis and metastatic potential [4, 5, 7, 15]. In our study, the mean number of CD163 positive cells was increased in successive stages of the Breslow scale, and was significantly increased in group III and IV compared to group I. Previous reports also documented that a high density of M2 macrophages in melanoma correlates positively with Breslow's thickness [5]. Thus, our findings are in concordance with general knowledge in this field suggesting, that higher infiltration of M2 macrophages is associated with more advanced melanomas. Moreover, the results of our investigation show that the number of CD163 positive cells was associated inversely with patient survival. In line with our findings, Jensen et al. [28], and Falleni et al. [5] also reported that the density of M2 macrophages was correlated with poor overall patient survival, supporting the important role of CD163 positive macrophages in the melanoma development and prognosis.
Literature data revealed that TAMs play an important role in the process of angiogenesis also. TAMs tend to accumulate in hypoxic areas of tumors and produce multiple cytokines and chemokines that are known to accelerate angiogenesis such as VEGF, FGF, TNF-a, TNF-b, and CXCL [15, 16, 20, 29]. Increased number of TAMs were documented and positively associated with angiogenesis in several types of human cancers [30, 31, 32, 33]. In accordance with previous findings, we demonstrated that in cases of melanoma with higher TAMs infiltration, the microvessels density was also increased. The correlation between the presence of CD68 and CD163 positive macrophages and microvessels density described in our study is in concordance with current literature data [15, 16, 29, 30], and suggest that a high macrophages count may play an important role in angiogenesis.
By processing our data we managed to show some promising correlation between MVD and survival times of melanoma patients. Unfortunately, more thorough statistical analysis using the Cox proportional hazard model did not reveal expected results. Interestingly, literature data revealed many discrepancies concerning association between the microvessels density and patient's overall survival. Pastushenko et al. [34] revealed that the rate of intratumoral blood vessel proliferation was inversely associated with overall survival of patients with melanoma. On the other hand, Storr et al. [35] documented that angiogenesis estimated by microvessels density was not associated with overall survival. We should take into consideration that studies on human samples carries the intrinsic limitations, due to the biological variability of lesions, different number of studied cases and methodological issues (antibodies used, methods of evaluation, etc). We suggest that further, more homogenous and unified studies are necessary to make a final conclusion regarding the associations between microvessels density and survival of melanoma patients.
Tumor microenvironment immune cells are presently very promising subjects for the search of new prognostic factors and possible targeted therapies in melanoma. The present study documented that the higher density of CD68 and CD163 positive macrophages was positively associated with angiogenesis and Breslow depth. Moreover, the number of CD163 positive macrophages was associated inversely with patient's survival. Unfortunately, our study did not reveal correlation of MVD with survival times. Further studies are required to better understand relationships between individual cells of microenvironment, angiogenesis and tumor progression.
This work was supported by grant of Medical University of Lodz 502-03/6-038-01/502-64-085-17.
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6. Postovit LM, Seftor EA, Seftor REB, et al. Influence of the microenvironment on melanoma cell fate determination and phenotype. Cancer Res 2006; 66: 7833-7836.
7. Quail DF, Joyce JA. Microenvironmental regulation of tumor progression and metastasis. Nat Med 2013; 19: 1423-1437.
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10.	Gordon S. Alternative activation of macrophages. Nat Rev Immunol 2003; 3: 23-35.
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17.	Elder DE, Massi D, Scolyer R, et al. WHO Classification of Skin Tumours. 4th ed. Lyon, IARC 2018; 65-76.
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19.Spaw M, Anant S, Thomas SM. Stromal contributions to the carcinogenic process. Mol Carcinog 2017; 56: 1199-1213.
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22.	Kim S, Cho SW, Min HS, et al. The Expression of Tumor-Associated Macrophages in Papillary Thyroid Carcinoma. Endocrinol Metab 2013; 28: 192-198.
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26.	Venza M, Visalli M, Beninati C, et al. Cellular mechanisms of oxidative stress and action in melanoma. Oxid Med Cell Longev 2015; 2015: 481782.
27.	Spatz A, Batist G, Eggermont AM. The biology behind prognostic factors of cutaneous melanoma. Curr Opin Oncol 2010; 22: 163-168.
28.	Jensen TO, Schmidt H, Møller HJ, et al. Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma. J Clin Oncol 2009; 27: 3330-3337.
29.	Pollard JW. Tumour-educated macrophages promote tumour progression and metastasis. Nat Rev Cancer 2004; 4: 71-78.
30.	Valkoviæ T, Dobrila F, Melato M, et al. Correlation between vascular endothelial growth factor, angiogenesis, and tumor-associated macrophages in invasive ductal breast carcinoma. Virchows Arch 2002; 440: 583-588.
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32.	Straume O, Akslen LA. Expresson of vascular endothelial growth factor, its receptors (FLT-1, KDR) and TSP-1 related to microvessel density and patient outcome in vertical growth phase melanomas. Am J Pathol 2001; 159: 223-235.
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34.	Pastushenko I, Van den Eynden GG, Vicente-Arregui S, et al. Increased Angiogenesis and Lymphangiogenesis in Metastatic Sentinel Lymph Nodes Is Associated With Nonsentinel Lymph Node Involvement and Distant Metastasis in Patients With Melanoma. Am J Dermatopathol 2016; 38: 338-346.
35.	Storr SJ, Safuan S, Mitra A, et al. Objective assessment of blood and lymphatic vessel invasion and association with macrophage infiltration in cutaneous melanoma. Mod Pathol 2012; 25: 493-504.
Copyright: © 2019 Polish Association of Pathologists and the Polish Branch of the International Academy of Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.


Many people tend to view addiction as something only affects the young and middle-aged. In reality, substance use disorder is a very real threat to the elderly community – known as Elderly Substance Abuse.
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Q: Decide threshold for LM to filter out ill-formed sentence I have a corpus of sentences. This corpus contains well-formed as well as ill-formed sentences. I want to filter out ill-formed sentences from the corpus.
Ill-formed sentences are of two types :

*

*Sentences with grammatical errors, Ex : "What are the symptom of fever ?"

*Incoherent sentences, Ex : "How do you cure apple ?"

The first thing which I tried was Language Model. I used the perplexity scores as well LM probability to decide which ones better. In perplexity case lower the better and in LM probability case, higher the better.
But the problem I am facing with is how to decide the threshold of the value for perplexity and LM prob?
Looking at the scores seems there's a significant overlap in distribution of good and bad sentences.
I also don't have a labelled dataset, and hence I can't build a classifier where features would be LM Prob and Perplexity.
Any ideas is highly appreciated.

redpajama_set_name: RedPajamaStackExchange


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Chaguanas/Sando maxis relocated to Short St
Darlisa Ghouralal Created : 2 October 2017 T&T News
Chaguanas maxis in queue at their temporary location at Short Street, San Fernando
Green band maxi taxis have been temporarily relocated from St James Street to Short Street in San Fernando.
San Fernando Mayor Junia Regrello made the announcement on Friday as part of a solution to traffic congestion in the city.
The San Fernando City Corporation altered the route of allowing vehicles to enter High Street from St James Street, with a recommendation that this section of Penitence Street be converted into a two-way traffic lane, allowing traffic to enter and exit High Street.
The public has been invited to submit their comments on these changes.
Green band maxis run the San Fernando to Chaguanas/Chaguanas to San Fernando route.
President of the Route 3 Maxi Taxi Drivers' Association Julien St George told LoopTT the City Corporation initially suggested King's Wharf as a temporary location, however, the Association rejected the proposal on the basis that the area is known for crime and raised concerns for the safety and security of commuters, as they would have to trek further to get to the maxi taxi stand.
Short Street was then chosen as a temporary location.
St George said while the move is not ideal, the maxi taxi operators are willing to make it work.
"We expressed our reluctance and presented reasons why, and we were subsequently relocated to Short Street. It is not perfect, but it is a better choice than the Wharf.
So far, we have cooperated in relocating to Short Street."
"We are willing to make it work at this location. Sometimes when you move into a new environment, it might not be 100 percent, but we are willing to work with the City Corporation."
"They have their reasons why they wanted St James Street for the new traffic plan," he added.
St George noted that they were not consulted on the move, which moves them even further away from the catchment area of Upper High Street, Library Corner.
He's appealing with the Mayor to carry out the necessary consultation with stakeholders to ensure that there is no disruption in service.
maxi taxi stand
green band maxis
San Fernando Mayor Junia Regrello
Julien St George
Route 3 Maxi Taxi Drivers' Association
Maxi Taxi Advisory Committee appointed
Police nab maxi taxi robber
US concerned about Trinis traveling to 'violent' countries

redpajama_set_name: RedPajamaCommonCrawl


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Q: How short setIntervals Should I do? I'm working with a facebook tab-page. Using javascript I how far down a user has scrolled in it's parent page (i'm inside an iframe and recieve the value via an api)
This is how I read the values. Right now though, it's a bit laggish. How often can I set the interval to? Is it possible and/or recommendet to go below 1ms?
setInterval(function(){
    FB.Canvas.getPageInfo(function(info){
        $offset=info.scrollTop+'px';
        console.log($offset);
        $('#footer').css("top",$offset);
    });
},50);


The average fee for attending a top private school has risen past £17,000 a year for the first time, despite climbing at the slowest rate for more than 20 years, according to the annual census of leading independent schools.
Data collected from the 1,300 members of the Independent Schools Council (ISC) – an umbrella body that ranges from famous public schools such as Eton and Harrow to hundreds of smaller prep schools – showed that a child attending a private secondary school as a day pupil would cost around £15,000 a year, while a boarder would pay £33,000.
The figures showed an increase of 3.3% compared with 2017, which the ISC said was the slowest annual rate of increase since 1994. However, independent school fees have outstripped the rate of inflation over that period.
The average annual fee for a day pupil was around £13,000 three years ago but 10 years ago the same education cost £9,600 a year. Boarding fees have risen by 50% in the last 10 years, having cost on average £22,000 in 2008.
In comparison, state schools in England receive around £4,000 to £6,000 per pupil depending on location and additional allowances for language and disadvantage, as well as access to funds for buildings and maintenance.
Despite the increases in fees the appetite for a private education seems stable, with a rise in students attending the council's member schools to 529,000, the highest number since its records began in 1974. Much of the increase was accounted for by new schools joining the group.
The census showed another strong increase in the number of pupils coming from China and Hong Kong, which make up the largest group of non-British nationals in independent schools. Together they account for 14,000 of the 53,000 non-British pupils, including those with parents living inside and outside the UK.
Pupils from Russia account for 2,800 of the total, while there are just under 4,000 from the US.
Ethnicity data showed that ISC schools in England had a slightly higher proportion of pupils from non-white British ethnic groups, compared to state schools in England.
The ISC said that its member schools were offering increasing amounts of fee assistance worth nearly £1bn a year to families of pupils, to help them meet the heavy costs involved. The census found that 171,000 pupils were receiving help of some sort with their fees.
"In recent years independent schools have raised money to subsidise the fees of lower income families, widening access. Students from disadvantaged backgrounds, who are on bursaries at ISC schools, often go on to secure further financial help in order to attend top UK universities," said Barnaby Lenon, the ISC chair and a former head of Harrow school.
In recent years independent schools have come under attack by both Labour and Conservative MPs for hindering social mobility, although the government has dropped plans to force private schools to support state schools or risk losing their charitable status.
The census notes that only three-quarters of its member schools now operate as charities, with an increasing proportion either dropping or foregoing charitable status.


text:
Trisomi är en typ av kromosomrubbning, som innebär att man har tre exemplar i stället för det normala två av någon kromosom i sina celler. Den vanligaste trisomin är den för kromosom 21, vilket ger upphov till Downs syndrom. En äldre term för Downs syndrom är mongolism och då kallades en person som hade syndromet för mongoloid eller mongolid. Uttrycket uppfattas idag som kränkande. Uttrycket mongolism uppkom på grund av det så kallade mongolvecket. Vecket vid ögat som personer med Downs syndrom har, vilket också är vanligt hos folk med östasiatisk bakgrund.

Andra trisomier är Edwards syndrom (trisomi 18) och Pataus syndrom (trisomi 13). Trisomierna brukar medföra utvecklingsstörning och kroppsliga missbildningar. Deras allvarlighet beror på typ av trisomi (Downs syndrom är lindrigt i jämförelse med de andra två exemplen, medan trisomier på andra kromosomer ger så grava missbildningar att de oftast ger upphov till missfall), och därtill på "grad" av trisomi. Det finns nämligen förutom vanlig trisomi även mosaicistisk trisomi, vilket innebär en extra kromosom i vissa men inte alla kroppens celler, och partiell trisomi, vilket innebär en del av en extra kromosom i cellerna i stället för en hel sådan. Sådana trisomiformer kan innebära lindrigare handikapp.

Kromosomavvikelser

redpajama_set_name: RedPajamaWikipedia


Uploaded by NURHAYATI in Waco at Tuesday, August 12, 2014. The Remarkable Door images on this page are the Remarkable pic that we served for you with a dimension of 314 x 250 pixels. Natural Sliding Blind Doors above are the same as what I saw when I visited my mother in law�s hotel in Minneapolis, United State of America. Don�t be hesitate; you can also see the entire writing in the Trending Styles of Vertical Blinds for Glass Doors which contains pic that I took during a holiday to various states such as Denmark, Lithuania, and Panama.
Feel free to use the interior wood pic that I provided even though you are in tropical or desert states like Burma, Lebanon, and Lesotho. Download by right clicking your mouse right on the Remarkable pic, then give this high definition pic to your home decorator to be applied soon.


All this talk about frameworks the past two Wednesdays, and yet we did not talk about what qualities you should look for when you chose to use one. Many businesses have their favourites and ask that you as a potential employee are comfortable around them and can work with them. In this article we are going to outline the five questions you should ask yourself when determining which framework to use on a project. Does the framework have enough popularity? Popularity will open a lot of opportunities for you personally. It will offer you support from a community that has a variety of skills and experience. It will also offer you up to date files, with glitches fixed quickly. There is also a higher chance that companies are looking for people familiar with this framework.


text:
Q: Is there a canonical fork of mustache.js? I'm looking at pulling mustache.js into a project, and a quick glance at its network graph revealed that it has a ton of heavily-committed-to forks.
Are any of these considered canonical/preferable? Or am I best off starting with a download of janl's original distribution until I need something from a fork?

A: This is always a tough one.  If the root repo does what you need and doesn't seem buggy, it's best to start there.  Unless the owner of the root repo has specifically stated that active development has been passed on to a specific fork.  You can always explore forks later, or even make your own fork and pull the stuff you want in to it.


text:
Escharina vulgaris är en mossdjursart som först beskrevs av Karl von Moll 1803. Enligt Catalogue of Life ingår Escharina vulgaris i släktet Escharina och familjen Escharinidae, men enligt Dyntaxa är tillhörigheten istället släktet Escharina och familjen Schizoporellidae. Arten är reproducerande i Sverige. Inga underarter finns listade i Catalogue of Life.

Källor

Mossdjur
vulgaris


text:
Q: Allocate node size based on longest overlay version I'd like to make a node whose text content changes between overlays. Of course, the different contents have different sizes, so the node itself changes size between overlays. I'd like to avoid that by allocating the maximum needed size.
Here's some code to demonstrate what I'd like. Because the Bar text caption's size changes, the whole picture jumps around. First, I try using \only for the overlays, but that means only the current one takes up space. Then, I try using \visible, but then all of them always take up space.
\documentclass{beamer}
\usepackage{tikz}
\begin{document}
\begin{frame}{Try 1}

  \begin{center}
  \begin{tikzpicture}
    \draw (0,0) node[draw]{Foo};
    \draw (1,0) node[right]{Bar{\only<2>{short}}{\only<3>{much much longer}}};
  \end{tikzpicture}
  \end{center}
\end{frame}

\begin{frame}{Try 2}

  \begin{center}
  \begin{tikzpicture}
    \draw (0,0) node[draw]{Foo};
    \draw (1,0) node[right]{Bar{\visible<2-3>{\visible<2>{short}}{\visible<3>{much much longer}}}};
  \end{tikzpicture}
  \end{center}
\end{frame}
\end{document}

A: If you know the longest text beforehand (and are willing to specify it in the document) you can just add the option
text width=width("Barmuch much longer")

to the node.
As you've noticed, this will still "jump around" (vertically at least) since the last one has that g that has a descender (a depth in TeX language).
Since you implicitly specify with right that the node should use its west anchor it will be vertically aligned at the center of the text box.
You will either anchor your nodes via the base or the mid anchors west mid, mid, east mid, base west, base, base east or text or also specify the text depth and the text height for your nodes, too.
Adding a \strut (which also can be added automatically to your nodes via font=\strut) extends the text box vertically enough so that normal text does not protrude.
But you can also use text depth and text height again:
\tikzset{
  max text/.style={
    text width ={width("#1")},
    text height={height("#1")},
    text depth ={depth("#1")}},
}

with
node[max text=Barshourtmuch much longer]

At the end, it comes down to what your diagram needs, how you place the nodes, if you draw their border and whether you connect lines to them.

Adding a \strut is similar to doing
text depth=.3\baselineskip,
text height=.7\baselineskip,

although \strut will allow the text box to grow vertically while text depth and text height will result in fixed sizes.

A: You could split your node into two nodes and have the long node occupy space on all slides by making it invisible:
\documentclass{beamer}
\usepackage{tikz}

\usetikzlibrary{overlay-beamer-styles}

\begin{document}
\begin{frame}{Try 1}

  \begin{center}
  \begin{tikzpicture}
    \node[draw] (0,0) {Foo};
    \node[right,visible on=<3>] at (1,0) {\strut Barmuch much longer};
    \node[right,visible on=<-2>] at (1,0) {\strut Bar\only<2>{short}};
  \end{tikzpicture}
  \end{center}
\end{frame}

\end{document}


BLOOMFIELD HILLS, MICHIGAN and NEW YORK, NEW YORK--(Marketwire - Oct. 21, 2011) - OMERS Private Equity, the private equity arm of the OMERS Worldwide group of companies, has partnered with existing management to purchase GEDC Super Holdings Inc. (together with its affiliates, "Great Expressions" or the "Company") from affiliates of private equity firm Audax Group.
Founded in 1982 and headquartered in Bloomfield Hills, MI, Great Expressions is a leading dental practice management ("DPM") company, with 152 affiliated dental offices in seven states across the Midwest, South and Northeast. In the past three years, the Company has grown by adding and successfully integrating 79 offices through 10 acquisitions. With over 1,800 clinical and support staff, Great Expressions' affiliated dental offices provide all facets of dental services, including general and preventative care focused on the highest quality patient care, affordable prices and convenient locations.
"The benefits that the DPM industry offers to its patients, dentists and payors makes it an attractive investment opportunity for OMERS," said Michael Graham, Senior Managing Director and Head of U.S. Private Equity for OMERS. "In particular, Great Expressions' focus on quality care, affordability and convenience has led to superior operational performance as well as compelling growth. We look forward to working closely with management to support the continued growth of Great Expressions."
"My team and I are excited to work with OMERS Private Equity and to continue growing Great Expressions," said Rich Beckman, CEO of Great Expressions. "We are excited to partner with a team who has experience in healthcare services and a long-term approach to investing. Great Expressions intends firstly to grow in its core markets of Florida, Georgia and Michigan, and then to seek attractive growth opportunities in its other markets. We believe OMERS Private Equity's knowledge of the DPM industry and commitment to our strategy will enhance our expansion."
Ares Capital Corporation and General Electric Capital Corporation acted as co-lead arrangers and bookrunners for the credit facility. Weil, Gotshal & Manges LLP acted as legal counsel for OMERS Private Equity.
Great Expressions is a leading provider of comprehensive and affordable dental services through its affiliated offices, including general and preventative care, cosmetic, endodontic, oral and maxillofacial surgery, orthodontics, pedodontics and periodontics. Through a network of 152 dental offices staffed with over 500 well-trained dentists and hygienists in Connecticut, Florida, Georgia, Massachusetts, Michigan, Ohio and Virginia, Great Expressions is committed to providing affordable dental care while maintaining clinical excellence and convenience for its patients. For further information visit: www.greatexpressions.com.


text:
Terrestrial based tracking using the time difference of arrival to calculate position. Should give high positional accuracy during most phases of flight, but position errors can sometimes occur. The ground speed is calculated and can sometimes be incorrect, especially during turns and at low altitudes. Vertical speed is also calculated, so errors can sometimes occur. Altitude data come from the transponder and should be correct. Read more
Orlando Continental Plaza Hotel $39+ Clarion Inn & Suites At International Drive $58+ Monumental Hotel Orlando $60+ Red Lion Hotel Orlando - Kissimmee Maingate $63+ Clarion Hotel Orlando International Airport $64+ Rosen Inn International $65+ Rodeway Inn International Drive $66+ Rosen Inn at Pointe Orlando $68+ Rosen Inn, Closest To Universal $69+ Baymont by Wyndham Orlando Universal Blvd $72+ Best Western Orlando Gateway Hotel $85+ Westgate Town Center Resort $87+ Avanti International Resort $88+ Rosen Plaza on International Drive $89+ DoubleTree by Hilton at the Entrance to Universal Orlando $94+ Grande Villas Resort By Diamond Resorts $98+ Holiday Inn & Suites Across From Universal Orlando $98+ Sheraton Vistana Villages Resort Villas, I-Drive/Orlando $100+ Fairfield Inn & Suites by Marriott Orlando Lake Buena Vista in The Marriott Village $100+ Coco Key Hotel & Water Park Resort $101+ Rosen Centre Hotel $104+ DoubleTree by Hilton Orlando at SeaWorld $104+ Sheraton Vistana Resort Villas, Lake Buena Vista/Orlando $107+ Wyndham Orlando Resort $110+
I booked a 3.5/4 star hotel at a great rate, 3 months prior to our New York trip. The trip was a birthday present to my son and it was our first time to the Big Apple. I had not received any communications from Travelocity about any changes but, when we finally arrived at the hotel, we found out the reservation was cancelled by their sister company, Expedia. On our first day in New York, I spent 4 hours on the phone with customer service who kept promising that they would fix it. We were tired and it was late so, we spent double the amount to stay the night at the hotel, who was nice enough to cut their rate in half for us. The following day, I called Travelocity for a follow up and was told a mixture of "you're booked, you're not booked" answers. They had not resolved the issue at all and we were faced again with paying double the amount to stay at the hotel. I finally demanded to speak to a supervisor who, after being on the phone for 6 hours this time, was able to book us to another hotel for the remainder of our trip. We couldn't check in until the next day so, we had to pay for another night at the original hotel. Travelocity did credit us for most of the differences in hotel prices but they made it impossible for us to fully enjoy our vacation! The stress alone was taxing and I will never use them or Expedia again!!
We've tried to make booking an affordable Price hotel simple. Best place to start is by having a look through the top 10 cheap hotels trending right now in Price in the box to the right. Make sure you use our traveller reviews to ensure you know exactly what you're getting out of your hotel room. It's understandable to be cautious when looking for cheap hotel rooms - you don't want to pay more than you should, but you also want to make sure the room is comfortable, clean, etc. That's one of the many reasons we take customer feedback so seriously (not only do we show you our customer's hotel reviews, we also use Expedia's and TripAdvisor's!). To help you quickly narrow your search, here's a list of the best cheap Price hotels for:
In August 2012, Travelocity faced a viral controversy when it offered a $200 coupon code to attendees at the National Federation of the Blind annual conference in Dallas. After the NFB posted the code on Twitter without mentioning the attendee restriction, Travelocity re-tweeted it without noticing the error but deleted the tweet a day later. After some travel blogs and message boards resposted the code, many ineligible travelers used the code.[30] Travelocity responded by cancelling all trips that used the code who weren't on the list of attendees at the NFB annual conference. This resulted in a barrage of complaints from customers angry to see their trips suddenly cancelled.[31]
In March 2002, Travelocity acquired last minute travel specialist Site59.com.[9] The CEO and founder of Site59, Michelle Peluso, joined Travelocity with the acquisition as senior vice president, product strategy and distribution. Peluso became Travelocity's COO in April 2003 and was then named president and chief executive officer of Travelocity in December 2003.[3] Many members of Peluso's former management team at Site59 were appointed to senior management positions at Travelocity including Jeffrey Glueck (Chief Marketing Officer), Tracey Weber (President, North America), Josh Hartmann (Chief Technology Officer) and Jonathan Perkel (Senior Vice President and General Counsel).[10]
Please be aware that construction is in progress to make improvements to the Terminal Entrance, Roadways and Terminal Parking Lots to provide additional parking spaces for free public parking and to improve the traffic flow to and from the Terminal area. Construction is underway and will continue through the end of November 2018. Signage will be in place for direction along with standard barricading for areas currently under construction for your guidance and safety. Thank you for using Abraham Lincoln Capital Airport and being aware of the construction while you are at at the Airport.
Flight Orlando - Washington (MCO - DCA) $97+ Flight Minneapolis - Washington (MSP - IAD) $107+ Flight Minneapolis - Washington (MSP - DCA) $117+ Flight New York - Washington (JFK - DCA) $127+ Flight New York - Washington (LGA - DCA) $147+ Flight Boston - Washington (BOS - DCA) $155+ Flight Boston - Washington (BOS - IAD) $161+ Flight Fort Lauderdale - Washington (FLL - DCA) $168+ Flight Denver - Washington (DEN - DCA) $173+ Flight Santa Ana - Washington (SNA - DCA) $182+ Flight Chicago - Washington (ORD - DCA) $186+ Flight Dallas - Washington (DFW - DCA) $198+ Flight Los Angeles - Washington (LAX - DCA) $204+ Flight San Francisco - Washington (SFO - DCA) $206+
Central Park West Hostel $53+ Broadway Hotel And Hostel $56+ Blue Moon Boutique Hotel $65+ Pod 51 $101+ Hotel Pennsylvania $104+ Club Quarters Hotel, Wall Street $112+ Holiday Inn Manhattan-Financial District $113+ The Watson Hotel $116+ DoubleTree by Hilton New York Times Square West $126+ Night Hotel Times Square $127+ The Gallivant Times Square $127+ DoubleTree by Hilton New York City - Financial District $128+ Row NYC $131+ The Manhattan At Times Square Hotel $131+ Hotel Edison $136+ Hudson New York, Central Park $140+ Paramount Hotel $143+ Empire Hotel $145+ MOXY NYC Times Square $152+ The Roosevelt Hotel $153+ YOTEL New York $156+ Dream Midtown $159+
Flight Houston - Chicago (HOU - ORD) $55+ Flight Denver - Chicago (DEN - ORD) $79+ Flight Boston - Chicago (BOS - ORD) $90+ Flight New York - Chicago (LGA - ORD) $91+ Flight Phoenix - Chicago (PHX - ORD) $94+ Flight Dallas - Chicago (DFW - ORD) $97+ Flight Minneapolis - Chicago (MSP - ORD) $97+ Flight Washington - Chicago (BWI - ORD) $101+ Flight Atlanta - Chicago (ATL - ORD) $108+
Flight Atlanta - Newark (ATL - EWR) $99+ Flight Fort Lauderdale - Newark (FLL - EWR) $114+ Flight Fort Lauderdale - New York (FLL - LGA) $124+ Flight Chicago - New York (ORD - JFK) $133+ Flight Houston - Newark (IAH - EWR) $133+ Flight Dallas - New York (DFW - LGA) $134+ Flight Houston - Newark (HOU - EWR) $140+ Flight Denver - New York (DEN - LGA) $141+ Flight Miami - Newark (MIA - EWR) $141+ Flight Los Angeles - Newark (LAX - EWR) $145+ Flight Los Angeles - New York (LAX - LGA) $157+ Flight Orlando - New York (MCO - LGA) $157+ Flight Seattle - Newark (SEA - EWR) $165+ Flight Chicago - Newark (ORD - EWR) $175+ Flight Houston - New York (HOU - LGA) $190+ Flight Dallas - New York (DFW - JFK) $197+ Flight San Francisco - New York (SFO - LGA) $219+ Flight Dallas - Newark (DFW - EWR) $221+ Flight Portland - Newark (PDX - EWR) $232+ Flight San Francisco - Newark (SFO - EWR) $236+ Flight Los Angeles - New York (LAX - JFK) $237+ Flight Ontario - New York (ONT - JFK) $237+ Flight San Francisco - New York (SFO - JFK) $237+ Flight Phoenix - New York (PHX - JFK) $247+
Visiting Las Vegas on a budget? Consider traveling mid-week rather than over a weekend, when the city will be less busy and prices are more likely to be cheap. Traveling early in the week will help you avoid crowds and you will have more choice of the hotels and rooms you are interested in. Keep in mind that Thursday in Las Vegas has started to become just as popular as Friday and Saturday.
Flight Boston - London (BOS - LHR) $312+ Flight New York - London (JFK - LGW) $317+ Flight Newark - London (EWR - LHR) $330+ Flight New York - London (JFK - LHR) $349+ Flight Dallas - London (DFW - LHR) $367+ Flight New York - London (LGA - LHR) $372+ Flight Chicago - London (ORD - LGW) $374+ Flight Chicago - London (ORD - LHR) $387+ Flight New York - London (JFK - LCY) $390+ Flight Newark - London (EWR - LCY) $390+ Flight Newark - London (EWR - LGW) $399+ Flight San Francisco - London (SFO - LHR) $417+ Flight Washington - London (IAD - LHR) $422+ Flight Los Angeles - London (LAX - LHR) $425+
While it's true that hotels in Las Vegas are famous for their gambling, that doesn't mean it's impossible to stay in a non-gaming hotel without a casino, even on The Strip. Some examples of hotels without a casino include the Four Seasons Hotel, the Elara, and the Mandarin Oriental. These are great choices for family vacations or even for individuals not interested in gambling.
Travelocity was created in 1995 through a joint venture between Worldview Systems Corporation and Sabre Holdings. The founding team at Worldview conceived of the idea in 1994 as an extension to their online travel database offering which had been distributed through Sabre, Bloomberg, AOL and many others. The founding team at Worldview joined with distribution partner Sabre in a 50-50 JV that resulted in the development and launch of Travelocity in 1995-1996. The founding members of the Travelocity team, responsible for the conception, development and launch at Worldview were: Steve Baloff (Founder, CEO), Sam Haugh (VP Operations), BD Goel (VP Engineering), Neil Checkoway (VP Marketing), Steve Bengston (VP Business Development), Helen Zia (Editor-in-Chief) and Katherine Chesbrough(CFO). Later in 1996, Worldview's investors (Advanced Publication and Ameritech) sold their stake in Travelocity to a subsidiary of Sabre Holdings and was run by long-time Sabre information technology executive Terry Jones.[4] As one of the pioneers of web-based disintermediation, Travelocity.com was the first website that allowed consumers the ability to reserve, book, and purchase tickets without the help of a travel agent or broker.[4] In addition to airfares, the site also permits consumers to book hotel rooms, rental cars, cruises and packaged vacations.[3]
Vegas.com has the best deals on Las Vegas hotels of every type to help you find the perfect room that will fit your budget. Looking for a cheap stay in a clean cubby? No problem. How about suites of epic grandeur in luxury Las Vegas resorts? Yep, got 'em too. Before you make your Las Vegas hotel reservations read hotel reviews from people who've actually stayed there…so you'll know you're making the right choice.
All the airlines, airports, services and trademarks listed are the sole property of their respective owners. This site is not affiliated with any of the airlines listed above - but is a service to track flights. Should you have any questions for any of the individual airlines you are asked to contact them direct. If you feel there is an error with our flight tracking please feel free to contact us.
The Strip is where all the Las Vegas magic happens. Beyond the iconic welcome to Las Vegas sign, the world's biggest hotel-casinos dominate the view as far as the eye can see. Among thousands of square feet of utter extravagance, Bellagio's Casino asserts itself as king. Pause at the Fountains of Bellagio, which leap to light and sound shows with typical Vegas showiness. Once you enter this palatial complex, you'll be lost in a world of glittering lights, jingling slot machines, and stellar shows. Competing with Bellagio in the dramatic stakes is the Colosseum at Caesars Palace, a flamboyant theater which embodies the hotel's Roman theme, and it hosts the Strip's biggest entertainment shows. When you need a change of pace, head out of town. Red Rock Canyon is just outside Las Vegas. This Martian-like formation of hulking, copper-hued rocks is nature's spectacular answer to the man-made wonders of the Strip.
The Quisby $50+ Wyndham Garden Hotel Baronne Plaza $93+ The Whitney Hotel $99+ B on Canal $106+ Pelham Hotel New Orleans, La $116+ International House Hotel $118+ Royal St. Charles French Quarter/Downtown $119+ Royal Crescent Hotel $120+ Holiday Inn New Orleans-Downtown Superdome $122+ Chateau Hotel $122+ Holiday Inn Express New Orleans - St Charles $122+ Wyndham New Orleans - French Quarter $126+ French Market Inn $136+ Bienville House $141+
Search cheap hotels with KAYAK. Use the hotel finder to search for the cheapest hotel deal for all major destinations around the world. KAYAK searches hundreds of hotel booking sites to help you find hotels and book hotels that suit you best. Since KAYAK searches many hotel sites at once, you can find discount hotels quickly. Discover hotel discounts now and make your hotel reservation today.
It's rare to have a sizeable international airport just minutes from the main attractions of a place, but Las Vegas has always been a place to break the rules. Its airport handles flights from all over the world, and it's just 2 miles from the Strip. Free shuttle services and an onslaught of taxis await to whisk you to your hotel. Minutes later, when you're out exploring, there are numerous ways to get around. It can often be quicker to walk between casinos especially when the traffic's gridlocked, but taxis and buses ply the Strip and surrounding areas too.


Plants have always been a supreme source of drugs and India is endowed with a wide variety of them with high medicinal values. The Quorum Sensing (QS) quenching efficiency of various solvent extracts of Melia dubia seeds was investigated against uropathogenic Escherichia coli (UPEC) to screen the competitive inhibitor of SdiA, a transcriptional activator of quorum sensing in E. coli. In this study, potentiality of five different extracts of Melia dubia seeds for quorum sensing inhibitory activity was investigated against uropathogenic Escherichia coli (UPEC). Assays such as cell density, swarming motility, protein, protease, hemolysis, hemagglutination, hydrophobicity and biofilm inhibition were performed. Biofilm, hemolysis and swarming motility were found to be inhibited by 92.1%, 20.9 % and 48.52% respectively, when the medium was supplemented with 30 mg/ml of the ethanolic extract. GC-MS spectrum of the ethanolic extract showed an array of 27 structurally unlinked compounds with natural ligand C8HSL. The docking against QS transcriptional regulator SdiA was predicted by in silico studies and the ligand C6 showed significant activity with -10.8 GScore. In vitro and in silico docking analysis showed fairly a good correlation, suggesting that the ethanolic extract showed potency to attenuate quorum sensing of uropathogenic E. coli. Further studies by in vitro and in vivo strategies are necessary to foresee the quorum quenching effect of the ligands.
Quorum Sensing, quorum quenching, Melia dubia, uropathogenic E.coli, SdiA.
Quorum Sensing Laboratory, School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur – 613 401, Tamil Nadu, India.


1. Describe pharmacodynamics, pharmacokinetics, classification and the principles of drug administration.
2. Explain chemotherapy of specific infections and infestations and nurses responsibilities.
3. Describe antiseptics, disinfectants, insecticides and nurses responsibilities.
4. Describe drugs acting on gastrointestinal system and nurses responsibilities.
5. Describe drugs used on respiratory system and nurses responsibilities.
6. Describe drugs used on urinary system and nurse's responsibilities.
7. Describe drugs used in de-addiction, emergency, deficiency of vitamins and minerals, poisoning, for immunization and immunosuppressant and nurses responsibilities.
8. Describe drugs used on nervous system and nurses responsibilities.
9. Describe drugs used on cardio-vascular system and nurses responsibilities.
10. Describe drugs used on hormonal disorders and supplementation, contraception and medical termination of pregnancy and nurse's responsibilities.
11. Demonstrate awareness of common drugs used in alternatives system of medicine.


Showing results for tags 'vírus'.
I have quickmapsanddirectons.com virus on my computer. I have Malwarebytes Premium. I first tried uninstalling Chrome, then reinstalled it and I still have the virus "popping up". Can I get rid of this virus using Malwarebytes?
it added a lot of random files and a fake Ea app (which is the virus) i cant delete them cause it says that its a system file it also deleted some of my system32 files . please help me!
Damn i just got hit too, stop decrypt not working for this rumba *****, please help n solution guys.
I WAS HIT TOO .PLEASE HELP!
I got hit too, please help.. heellppp...!!!!
Probably infected - lost cause?
So I was trying to download a crack for a random Application and I did something stupid and disabled antivirus just to open the file I downloaded and I'm pretty sure that I got a virus/malware/something bad now. a lot of random stuff started to get downloaded to my pc and I cannot access apps like discord cuz it says I got no permissions and same when I try downloading Malwarebytes or other antiviruses. idk what to do please help.
Malware, unable to acess windows defencer even after a full scan.
Hello, last week I have found 3 different processes bumping my CPU up to 100%. All of them were located in the temp folder but were named lsass.exe svchost.exe and nv… .exe. I figured they were not windows processes because they weren't located in the system32 folder and were run alongside the real processes. I have managed to eliminate them through Malwarebytes but I noticed my computer was still acting weird by asking for my onedrive and outlook password all the time. I was also unable to reactivate windows defender (even before I installed Malwarebytes), I kept getting a message that this is controlled by my IT administrator which doesnt make sense because Its a personal laptop. Also today my Facebook account got hacked and deleted so it raised my suspicion that there are definetly some left overs. If you could please provide assistance I would be forever grateful, just want my laptop back. Thank you!
my old laptop, Windows 7 is infected with a virus. I probably got it from inserting one too many Flash drives from my friends. It appears to be infected with some thing called "Files.bat" and a shortcut virus. I will also note that I found some processes in my Task manager that look suspicious. "hwbky32.exe" and "mldcn64.exe" If these are normal please tell me because I absolutely have no idea. So yeah, this is what my flash drive looks like when I insert it in my Laptop. Hope someone will help me solve this!
I got hit too :( ,my encrypted files are extremely essential what can I do now???


text:
import { IQuickPickSeparator } from 'vs/platform/quickinput/common/quickInput';
import { PickerQuickAccessProvider, IPickerQuickAccessItem, TriggerAction } from 'vs/platform/quickinput/browser/pickerQuickAccess';
import { localize } from 'vs/nls';
import { INotificationService } from 'vs/platform/notification/common/notification';
import { IDebugService } from 'vs/workbench/contrib/debug/common/debug';
import { IWorkspaceContextService, WorkbenchState } from 'vs/platform/workspace/common/workspace';
import { ICommandService } from 'vs/platform/commands/common/commands';
import { matchesFuzzy } from 'vs/base/common/filters';
import { withNullAsUndefined } from 'vs/base/common/types';
import { ADD_CONFIGURATION_ID } from 'vs/workbench/contrib/debug/browser/debugCommands';
import { debugConfigure } from 'vs/workbench/contrib/debug/browser/debugIcons';
import { ThemeIcon } from 'vs/platform/theme/common/themeService';

export class StartDebugQuickAccessProvider extends PickerQuickAccessProvider<IPickerQuickAccessItem> {

	static PREFIX = 'debug ';

	constructor(
		@IDebugService private readonly debugService: IDebugService,
		@IWorkspaceContextService private readonly contextService: IWorkspaceContextService,
		@ICommandService private readonly commandService: ICommandService,
		@INotificationService private readonly notificationService: INotificationService,
	) {
		super(StartDebugQuickAccessProvider.PREFIX, {
			noResultsPick: {
				label: localize('noDebugResults', "No matching launch configurations")
			}
		});
	}

	protected async _getPicks(filter: string): Promise<(IQuickPickSeparator | IPickerQuickAccessItem)[]> {
		const picks: Array<IPickerQuickAccessItem | IQuickPickSeparator> = [];
		picks.push({ type: 'separator', label: 'launch.json' });

		const configManager = this.debugService.getConfigurationManager();

		// Entries: configs
		let lastGroup: string | undefined;
		for (let config of configManager.getAllConfigurations()) {
			const highlights = matchesFuzzy(filter, config.name, true);
			if (highlights) {

				// Separator
				if (lastGroup !== config.presentation?.group) {
					picks.push({ type: 'separator' });
					lastGroup = config.presentation?.group;
				}

				// Launch entry
				picks.push({
					label: config.name,
					description: this.contextService.getWorkbenchState() === WorkbenchState.WORKSPACE ? config.launch.name : '',
					highlights: { label: highlights },
					buttons: [{
						iconClass: ThemeIcon.asClassName(debugConfigure),
						tooltip: localize('customizeLaunchConfig', "Configure Launch Configuration")
					}],
					trigger: () => {
						config.launch.openConfigFile(false);

						return TriggerAction.CLOSE_PICKER;
					},
					accept: async () => {
						await configManager.selectConfiguration(config.launch, config.name);
						try {
							await this.debugService.startDebugging(config.launch);
						} catch (error) {
							this.notificationService.error(error);
						}
					}
				});
			}
		}

		// Entries detected configurations
		const dynamicProviders = await configManager.getDynamicProviders();
		if (dynamicProviders.length > 0) {
			picks.push({
				type: 'separator', label: localize({
					key: 'contributed',
					comment: ['contributed is lower case because it looks better like that in UI. Nothing preceeds it. It is a name of the grouping of debug configurations.']
				}, "contributed")
			});
		}

		configManager.getRecentDynamicConfigurations().forEach(({ name, type }) => {
			const highlights = matchesFuzzy(filter, name, true);
			if (highlights) {
				picks.push({
					label: name,
					highlights: { label: highlights },
					accept: async () => {
						await configManager.selectConfiguration(undefined, name, undefined, { type });
						try {
							const { launch, getConfig } = configManager.selectedConfiguration;
							const config = await getConfig();
							await this.debugService.startDebugging(launch, config);
						} catch (error) {
							this.notificationService.error(error);
						}
					}
				});
			}
		});

		dynamicProviders.forEach(provider => {
			picks.push({
				label: `$(folder) ${provider.label}...`,
				ariaLabel: localize({ key: 'providerAriaLabel', comment: ['Placeholder stands for the provider label. For example "NodeJS".'] }, "{0} contributed configurations", provider.label),
				accept: async () => {
					const pick = await provider.pick();
					if (pick) {
						// Use the type of the provider, not of the config since config sometimes have subtypes (for example "node-terminal")
						await configManager.selectConfiguration(pick.launch, pick.config.name, pick.config, { type: provider.type });
						this.debugService.startDebugging(pick.launch, pick.config);
					}
				}
			});
		});

		// Entries: launches
		const visibleLaunches = configManager.getLaunches().filter(launch => !launch.hidden);

		// Separator
		if (visibleLaunches.length > 0) {
			picks.push({ type: 'separator', label: localize('configure', "configure") });
		}

		for (const launch of visibleLaunches) {
			const label = this.contextService.getWorkbenchState() === WorkbenchState.WORKSPACE ?
				localize("addConfigTo", "Add Config ({0})...", launch.name) :
				localize('addConfiguration', "Add Configuration...");

			// Add Config entry
			picks.push({
				label,
				description: this.contextService.getWorkbenchState() === WorkbenchState.WORKSPACE ? launch.name : '',
				highlights: { label: withNullAsUndefined(matchesFuzzy(filter, label, true)) },
				accept: () => this.commandService.executeCommand(ADD_CONFIGURATION_ID, launch.uri.toString())
			});
		}

		return picks;
	}
}

redpajama_set_name: RedPajamaGithub


Did you know that humor has proven therapeutic effects? That's right, finding reasons to smile and laugh, even in stressful situations, has been proven to be healthy. And one of the most stressful processes that many folks enter involves buying or selling real estate.
It seems there is a month to celebrate just about any type of awareness you could imagine. I'm not sure the exact procedure that it takes to create an "official" awareness campaign, but my recent discovery that April is Humor Awareness Month tickled my funny bone and led me to this blog focused on the more humorous side of real estate.
So, take a few moments to chuckle a little bit and take a deep breath, your real estate challenges could truly be more adventurous. And partnering with a real estate agent with deep experience can make all the difference in keeping humor on your side. I've been buying and selling real estate for over 25 years, learning the industry and having a few laughs along the way. Here are three steps that will help you keep things in perspective and make you chuckle as they highlight how great agents make this a fantastic industry.
A great agent knows how to position your property in the most positive light to attract traffic and interested buyers. The following story may be clever, but partnering with an agent with experience will help you find a new perspective on your circumstances. Experience in marketing and buyer desires makes all the difference!
It can be easy to feel discouraged during the staging, selling, and searching process. There are a lot of times when the number of requests can seem overwhelming. A great agent will help to inject optimism back into your world when things get busy with showings or staging, inspections or speed bumps.
There is an old saying that you shouldn't judge a book by its cover. There's another that proclaims that true beauty is on the inside. These surely apply to our human encounters, but when it comes to your property, appearances matter quite a bit to buyers. Staging, maintenance, and curb appeal all factor strongly into not only a buyer's consideration, but also how much they value the property when it comes time to make an offer. Good agents help you to decipher which items need attention, and which can wait until later. An experienced professional also has the insights to know where to spend dollars and time. This knowledge comes with years of real estate experience. For now, take a minute to smile at this joke.
Craving a bit more humor to round out your month? Check out The Lighter Side of Real Estate for fun real estate related stories and jokes. And remember, an experienced agent can keep you laughing when the unexpected happens during sale and purchase. A great agent provides peace of mind that lets you relax, knowing the details are taken care of and that everything is going to work out with your best interests in mind.
Call me for help with finding a maintenance free home and going through the process with a smile. I look forward to hearing from you soon!


This is a drawing by former FishBase staff member Robbie N. Cada.
Drawings done by Robbie Cada are public domain and can be used freely, however, FishBase would appreciate a proper indication of the source.
This work has been released into the public domain by its author, Robbie Cada. This applies worldwide.
Robbie Cada grants anyone the right to use this work for any purpose, without any conditions, unless such conditions are required by law.
current 20:24, 6 January 2015 640 × 480 (9 KB) GifTagger (talk | contribs) Bot: Converting file to superior PNG file. (Source: Ecnau_u0.gif). This GIF was problematic due to non-greyscale color table.


text:
Kumar Vikrant Singh
Congress appoints Kumari Selja as new state party president of Haryana
Just before the Haryana assembly elections, the Congress has handed over the command of the party in the state to Kumari Selja.
One man, one post in Congress: Sonia Gandhi set to take call
A senior Congress leader in the know of the development said, "Sonia Gandhi is set to decide on 'one man, one post' rule." He said a number of senior leaders hold multiple posts in the party.
Heavens open as Delhi bids adieu to Sheila Dikshit
Mortal remains consigned to flames with full state honours
After LS poll Congress faces financial crisis. Unpaid salaries, cost cutting become norm
Congress has reduced monthly budget of Congress Seva Dal from Rs 2.5 lakh to Rs 2 lakh. It has asked its women's wing, NSIU and Youth Congress to explore ways to reduce their expenses.
Why finding a new Congress chief won't be easy task
Earlier, he had made it clear to the Congress working committee on May 25 that a non-Gandhi should be given the post. He stressed that leaders should not suggest his sister Priyanka Gandhi Vadra's name.
Congress looks beyond Rahul Gandhi, CWC likely to elect interim party chief next week
The Congress has begun internal consultations for electing a provisional or interim party president. The decision is expected next week when the CWC is likely to meet and discuss the issue.
Rahul Gandhi refuses to accept Congress CMs' demand to continue as Congress chief
Chief Ministers of Rajasthan, Punjab, Madhya Pradesh, Chhattisgarh, and Puducherry met Rahul Gandhi and held a discussion on Congress's Lok Sabha poll debacle and also told him that the party workers want him to continue to provide leadership.
Congress review meeting for western UP ends in chaos, leaders get into heated argument over nepotism | WATCH
The review meeting called by senior Congress leaders Jyotiraditya Scindia and Raj Babbar to discuss party's poor performance in recently concluded Lok Sabha elections ended in chaos.
Sonia Gandhi upset with Rahul Gandhi's photo on Congress manifesto?
Sonia Gandhi looked upset at the Congress's manifesto launch event on April 2. Sources say she is unhappy with how the Congress manifesto carried Rahul Gandhi's image on the cover page.
Congress likely to move EC to defer release of PM Modi biopic
The party has said that if PM Modi's movie, slated for screening on April 5, is released during the Lok Sabha polls, it would violate the model code of conduct and influence free and fair elections.
Rahul Gandhi offers Lucknow ticket to Jitin Prasada, Rajya Sabha seat if he loses: Sources
Congress chief Rahul Gandhi has offered him to contest from Lucknow seat where he would be fielded against BJP leader and Home Minister Rajnath Singh and in case he loses, he would be given Rajya Sabha seat ticket.
No contest: Priyanka Gandhi may not even address rallies this Lok Sabha polls
Sources say Priyanka Gandhi will not speak at any of the future rallies too and let brother Rahul Gandhi do all the talking.
Rahul Gandhi cancels appointment of AICC secretary attached to Priyanka Gandhi 24 hours after appointing him
Congress president Rahul Gandhi cancelled the appointment of Kumar Ashish as an AICC secretary attached with Priyanka Gandhi Vadra, a day after he was assigned the responsibility.
Priyanka Gandhi to hit the road in Lucknow on February 11
This will be he first public appearance after taking charge as AICC general secretary of eastern Uttar Pradesh.
Digvijaya Singh, Jyotiraditya Scindia engage in verbal spat in presence of Rahul Gandhi
The two key leaders of the Congress in Madhya Pradesh sparred over ticket distribution and choice of candidates. Both had their own preferences and reservations.
Jaswant Singh's son Manvendra attacks BJP on joining Congress
Manvendra Singh alleged that the reason behind Goa Chief Minister Manohar Parrikar's illness was the file related to the controversial Rafale deal.
Congress's Bol Bum to counter BJP's Har-Har Modi in 2019 Lok Sabha election
Incidentally, both 'Har-har Modi' and 'Bol Bum' are inspired by deference to Lord Shiva. This is also coincidental that both Modi and Rahul Gandhi claim to be disciples of Lord Shiva.
When Rahul Gandhi told Congress leaders to learn from BJP, RSS
Congress president Rahul Gandhi on Sunday chaired the first meeting of the newly-constituted Congress Working Committee (CWC) in Delhi in while the party discussed the strategy for the upcoming 2019 Lok Sabha elections in order to defeat the Bharatiya Janata Party (BJP).
Rahul's warning for Tharoor: Will act against leaders who weaken Congress's fight
Rahul Gandhi today said he wouldn't hesitate to take action against Congress leaders whose statements weaken the party's "bigger fights", at a meeting of its working committee.
Walk the talk and ensure passage of Women's Reservation Bill, Rahul writes to PM Modi
Congress chief Rahul Gandhi has written a letter to Prime Minister Narendra Modi today (July 15) demanding the Bharatiya Janata Party (BJP) led NDA to take up the long-pending Women's Reservation Bill in the crucial monsoon session of the Parliament, sources told India Today.


The Swordfish trip we run every Friday Night has been doing great. We weren't able to run a trip this week, because we were getting our vessle through our annual reinspection. The Friday before however was a great trip. We started off by hooking into a really nice swordfish. Got him right to the boat and the fish turned his head just out of gaff range and pulled the hook. The fish waqs an estimated 150 lbs. We put nthe baits back out and got another bite from a big fish. We hooked it and fought it for quite a while. This swordfish was not as lucky as the last and we gaffed and boated him. A really nice one, we guessed to be another 150 pounder. We hooked another fish later that night, but we missed him. Every single bait we put out there got all shashed up. We had to change the spreads several times that night due to all the bites we were getting and all the bites looked to be swordfish slashes. A pretty good swordfish trip, although it would have been a superb swordfish trip if we got those other 2 fish.
The drift boat has been doing really good on their night time anchor trip. We've been catching yellowtail snapper by the bushel, and the larger mutton snappers have been biting pretty good too. Large by Lauderdale standards that is, most being 6-10 lbs. The drift boat has been geting good action in the day trips too, with lots and lots of bonitos, some small tunas, and a few big kingfish. We've also hit a few nice schools of dolphin, which have been coming in close lately, mostly schoolie size.
The sportfishing boats have been doing great. Mostly, with all the action on the reef, we've been trolling with planers and surface lures. The drone spoon has been the best bait lately and we've been catching lots of bonitos, kingfish and a nice blackfin tuna here and there. When the reef starts to slow down, we've been hitting the wrecks for some good action. Big amberjacks, and a few nice groupers have been caught.
On one trip this week we hit a big school of cobias on the bottom. On one of our 220′ shipwrecks we fish on, we drop a live blue runner to the bottom and get a nice bite. The fish comes shooting to the surface as we pull ahead with the boat, and I yell down to the mate, "it's probably a cobe', its right up on top." Sure enough, we get it closer to the boat and there's that big brown fish right on top, swimming towards the boat. We gaff him and the fish went ballistic in the boat. I jumped down the ladder and whacked him over the head a few times to welcome him aboard. I tell the customer that cobia aren't a rare fish but we don't catch them everyday. We go back to the same spot, and drop 2 lines down this time. Both get hit instantly and both were other big cobia. We boat those fish and we go back to the wreck for another drop and bammo, 2 more big cobia on. As we're fighting these 2 up, I see a huge boil on the surface of the water, about where the second cobia should have been. The fish starts screaming line off the reel and then, goes completely slack. I was like, "damn, he just got bit off." We got the other fish in the boat and wound up the one on the rod that got eaten. The head was eaten off just behind the gill plates. It must have been a giant bull shark or some other jurassic sea monster, but I never saw it. The head of the cobia that was eaten was bigger than the heads of any of the other cobias and the biggest one we got in the boat was 43 lbs, so it was a big one. 4 and a 1/2 cobias at the same spot. We went back to the wreck to try a couple more drops of coarse, but after the one got eaten, we never got another bite. It was a pretty awesome day of bottom fishing. And its kinda funny 'cause after the first one, I told the customer we don't catch them all that often, HAHAHA.
Shark are still biting also. A few big hammerheads being caught and some sand bar sharks are showing up too. Dolphin are biting a little in close, mostly schoolie size ones, but from the radio chatter I've heard there are some bigger one's offshore. I haven't been out there this week myself. Maybe I'll venture offshore tommorrow to give it a shot. Anyway, good luck out there guys. 'Till next week….


1:30 p.m., Francisco Street, Carson Transfer Station: Inside on the tipping floor, it's a circus of noises and smells.
Groaning trash trucks file into the cavernous building. Beep-beep-beep, they back up to a monstrous pile of debris, hydraulics are activated, the collection box tilts and another load of South Bay garbage tips out onto the floor.


When life presents you with too much kale, the sensible thing to do is to make a nice piping hot pot of Portuguese kale soup! Since we had a hard frost recently I thought it was time to head down to our field and harvest some sweetened up leaves. Since we are not able to sell any of our "flood-affected" produce, we have been viewing our farm as our own personal giant green grocer, there is much kale to be had, and that means lots of kale soup in my future! I can think of worse things. This soup is so great – it's got a lot of flavors I love, spicy chorizo, garlic, and a saltiness that accentuates the verdant flavor of the kale; it tastes exactly like Autumn, if autumn had a flavor. Treat yourself to more vitamins that you've had in a long time from a single dish, and cozy up with a bowl of caldo verde and some crusty buttered bread!
Heat stock pot on medium heat with 1 T. olive oil, until it shimmers.
red oil into the olive oil, and become fragrant.
Using a slotted spoon, remove chorizo and set aside.
and saute the onions and garlic until transluscent.
Add ¼ tsp. each salt and pepper at this time.
Add chopped potatoes to oily onions and garlic, stir to coat.
Add chicken stock and bring up to boiling.
Simmer for 15-20 minutes or until potatoes are fork-tender.
creating a creamy look to the soup.
Leave half the potatoes chunky.
Add chiffonaded kale to the pot, and stir to coat.
This will take 2 batches since the kale has so much volume.
top when serving), and bring to a boil.
Reduce heat, cover, and simmer until you can't stand it anymore.
Season to taste – it will need salt.
and with some hot crusty bread and butter for happiness.
Really nice with a pinot noir. Enjoy!


Radiation's ugly past is influencing its relatively safer future, an author says.
But, according to Tim Jorgensen, an associate professor of radiation medicine at Georgetown University and author of "Strange Glow: The Story of Radiation," the radiation levels that cell phones or microwaves expose us to are very low.
You also don't necessarily need to worry about airport scanning machines, according to Jorgensen. You're exposed to more radiation in the form of cosmic rays in one-minute of flight at 30,000 feet than you are in the scanner. Still, even pilots do not appear to be in danger — they experience only double the usual background radiation level that we all soak up each year.
A version of this story originated on Innovation Hub, a weekly radio show on innovation and the future.


text:
Why Low Fat is Killing You
Many have been led to believe that a low-fat diet will cause weight loss and raise their cholesterol. Unfortunately, this could not be further from the truth. Most people are still afraid of eating fat — any fat. They think a low-fat diet will help them lose weight and help prevent heart disease. In fact, the opposite may be true. Low-fat diets recommended by most nutritionists and dietitians are not only totally useless, they are also dangerous.
The New York Times has published a great article about the low fat obsession in America, "In 1988, the surgeon general, C. Everett Koop, proclaimed ice cream to a be public-health menace right up there with cigarettes. Alluding to his office's famous 1964 report on the perils of smoking, Dr. Koop announced that the American diet was a problem of "comparable" magnitude, chiefly because of the high-fat foods that were causing coronary heart disease and other deadly ailments. He introduced his report with these words: "The depth of the science base underlying its findings is even more impressive than that for tobacco and health in 1964."
In the case of fatty foods, that confident voice belonged to Ancel Keys, a prominent diet researcher a half-century ago (the K-rations in World War II were said to be named after him). He became convinced in the 1950s that Americans were suffering from a new epidemic of heart disease because they were eating more fat than their ancestors.
There were two glaring problems with this theory, as Mr. Taubes, a correspondent for Science magazine, explains in his book. First, it wasn't clear that traditional diets were especially lean. Nineteenth-century Americans consumed huge amounts of meat. The percentage of fat in the diet of ancient hunter-gatherers, according to the best estimate today, was as high or higher than the ratio in the modern Western diet.Second, there wasn't really a new epidemic of heart disease. Yes, more cases were being reported, but not because people were in worse health. It was mainly because they were living longer and were more likely to see a doctor who diagnosed the symptoms.
To bolster his theory, Dr. Keys in 1953 compared diets and heart disease rates in the United States, Japan and four other countries. Sure enough, more fat correlated with more disease (America topped the list). But critics at the time noted that if Dr. Keys had analyzed all 22 countries for which data were available, he would not have found a correlation. (And, as Mr. Taubes notes, no one would have puzzled over the so-called French Paradox of foie-gras connoisseurs with healthy hearts.)
The evidence that dietary fat correlates with heart disease 'does not stand up to critical examination,' the American Heart Association concluded in 1957. But three years later the association changed position — not because of new data, Mr. Taubes writes, but because Dr. Keys and an ally were on the committee issuing the new report. It asserted that 'the best scientific evidence of the time' warranted a lower-fat diet for people at high risk of heart disease."
1. Low-Fat Often Means High Sugar
To make foods fat-free or low-fat, food companies turn to additional processing, adding chemicals and fat replacers to keep textures and tastes similar to the original product. These additives (gums, inulin, maltodextrin, plydestrose, starch, modified food starch) are carbohydrate-based fat replacers. In many instances, fancy names for sugar.
2. Can Lead to Cardiovascular Problems
Did you know that having high levels of High Density Lipoprotein ( "good" cholesterol) is associated with a reduced risk of heart disease? Whereas, a low-fat diet leads to reductions in HDL, which cany lead to an increased risk of heart disease.
In a 2004 editorial in the Journal of American College of Cardiology, Sylvan Lee Weinberg, former president of the American College of Cardiology and outspoken proponent of the diet-heart hypothesis, said :
The low-fat, high-carbohydrate diet…; may well have played an unintended role in the current epidemics of obesity, lipid abnormalities, type 2 diabetes, and metabolic syndromes. This diet can no longer be defended by appeal to the authority of prestigious medical organizations.
3. Causes Hormone Imbalances
Did you know that many of the most important hormones are made from cholesterol? Cholesterol is needed for normal cell function and brain function; to strengthen your immune system and build brain and nerve tissue.
4. Causes Weight Gain
The Women's Health Initiative, the largest clinical trial of diet and body weight, discovered that 50,000 women on low-fat diets had no significant weight loss and no differences between those who followed low-fat, low-carb, and very- low-carb diets. In fact, most low-fat foods such as margarine, skim milk, and other reduced-fat products are loaded with calories, carbs, sugar, and processed flour that lead to weight gain.
5. Can Cause Depression and Anxiety
Most of our cells are made up of cholesterol, and over 60% of the brain is made up of fat. Thus, if you are not eating the right kind of fat, you are damaging your brain. \
Sarah Conklin, PhD, a postdoctoral scholar in Cardiovascular Behavioral Medicine, Department of Psychiatry at the University of Pittsburgh presented a study that found low levels of EPA and DHA were related to mood impairment.
We were able to show that individuals who consumed more omega-3 fatty acids in their diets had more gray matter volume in areas of the brain important for regulating mood. These results suggest that these specific fats, certainly not fat in general, may confer a protective effect against depression and other mood-related problems.
The Diet-Heart Myth: Cholesterol and Saturated Fat Are Not the Enemy
Shares of Bausch Health, formerly Valeant, rise 3% on FDA approval of psoriasis treatment
Measles nears record in US as the disease spreads in New York
Google vet launches an app for patients to record their doctor's notes
She Drink ONLY 1 cup of this tea, and her body has drastically changed


text:
No Sleep October: The Fog (1980)
Commentary, No Sleep October
Joe Shearer October 23, 2018 June 25, 2020
Joe Shearer is co-owner of The Film Yap (www.thefilmyap.com).
The Fog was one of the first horror movies I watched with regularity. A spooky, atmospheric ghost story about the people of a small town confronting the ghosts of their past, the film starts with a chilling fireside story from the legendary John Houseman and builds with the same creeping sense of dread as Carpenter's previous film, Halloween, leading to a similarly tense climax where the monsters and the town clash.
Perhaps the least splashy of John Carpenter's early run of films, The Fog largely holds up to the ravages of time and is a superior, if somewhat unheralded, entry into his canon. In the wake of Halloween, a film that shifted the course of the horror genre for decades to follow, Carpenter opted for a more traditional ghost story that has roots in the story of America and in the costs of building a new society. It may not hit the heights of Halloween, but it's a worthy entry in Carpenter's filmography — and one that brings back his favorite stars and bit players alike to populate Antonio Bay like Jamie Lee Curtis, Charles Cyphers, Annie Loomis and Tommy Lee Wallace contributing roles of various sizes and grades of importance.
Curtis, on a high after her breakout in Halloween, goes back to the same genre that would soon have her dubbed the "Scream Queen," with Terror Train, Prom Night, Road Games and Halloween II to immediately follow this.
The Fog takes place in Antonio Bay, California, a small town with a dark past, on the dawn of its centennial. The local legend of the sinking of a clipper ship marks the town's birth, but the circumstances behind the crash and death of all aboard is Antonio Bay's real legacy, one of willful cruelty and murder that will soon come back to literally haunt its residents.
Father Malone, Antonio Bay's priest and de facto leader, had been approached by the leader of a nearby leper colony, asking permission to set up a community nearby. Malone agreed but soon realized that such a development would bring shame upon his town and the prospect of having such people around would be an unpopular political move as well.
On the night the colony was to hit land, Malone shut down the town's lighthouse that was to guide the Elora Dane, the ship carrying the members of the colony, safely to port. Instead, he set a fire on the beach and when the Dane followed the light, the ship was destroyed by rocks near the shore. Everyone onboard died.
Over the next 100 years, most of the town forgot about this sinister development, but the descendants of those families mostly remained in Antonio Bay. Malone's grandson (played in the film by Hal Holbrook) took up the mantle of the town's priest, and it's he who discovers Antonio Bay's tragic past, which will soon become a curse.
As the clock strikes midnight on the date of the centennial, strange things begin happening. Car alarms go off, glass seems to break spontaneously. And a mysterious fog begins to roll toward shore.
Of course we know the fog contains the spirits of the dead lepers, betrayed and murdered by the town from which they sought only kinship. And 100 years later, they are coming back to exact their revenge.
The action chiefly follows a handful of Antonio Bay residents: Nick Castle (Tom Atkins), a local man who picks up hitchhiker Elizabeth Solley (Curtis); Malone, who discovers his grandfather's confessional diary that outlines his family's shameful legacy; and Stevie Wayne (Adrienne Barbeau), owner of the town's lighthouse and owner and DJ of the town's radio station, which plays old standards during the day and features her silky voice at night.
When night falls on the town, the fog returns, and it's here we see the scope of the destruction its ghostly inhabitants aim to wreak on Antonio Bay. There are no innocents, even kindly old Mrs. Kobritz (Regina Waldon), the babysitter for Stevie's young son, Andy (Ty Mitchell), who had earlier found the first evidence of the Elora Dane's return on the beach.
The special effects are rather simple but have an elegance and believability for the time. The zombies are seen almost entirely in shadow, save for a few "money shots" that rely on closeup. That Carpenter famously made "Halloween" on the cheap just a couple of years prior is still evident here. He clearly has a larger budget, but still employs a less-is-more approach. Kills feel violent and graphic but are largely bloodless, with hooks, swords and other sharp instruments used.
It's the air of menace and unstoppability of the fog that gives the film its effectiveness. The fog is a creeping, slow-moving behemoth but Carpenter does a good job of keeping the townspeople unaware until the fog blocks all of their major escape routes, pushing most of the film's principles toward the church (a parallel struggle, where the fog envelops Stevie at the lighthouse, is perhaps even more terrifying given its solo nature). The final scene, where Father Malone and the rest of the townspeople have to confront the literal sins of their fathers (or grandfathers and great-grandfathers, as it were) builds to a face-to-face confrontation, and a fitting, if somewhat silly, denouement.
Its spooky ambiance and a fun sins-of-the-father storyline, with just a touch or two of camp, give The Fog its appeal. For me, it's a rare relic of my childhood, one that is both a film of its time and one that can be enjoyed by today's audiences and passed down to additional generations … like a fond curse.
For most of his life, Evan Dossey has generally avoided horror films. The genre makes him profoundly uncomfortable. This means he has enormous gaps in his cinematic knowledge. Each year, he asks friends and family which essential horror movies he needs to see in order to fill those gaps and spends the better part of October agonizing over them, tossing and turning over them … and writing about them. This year, he's sharing the month with those friends and family — letting them offer their own thoughts about the tales that terrify (or perhaps just titillate) them. This is everyone's No Sleep October.
NO SLEEP OCTOBER 2018
Blue Sunshine — James Ledesma
Spoorloos (The Vanishing) — Andrew Kimmel
The Devil's Candy — Joshua Hull
FYFF Horror Marathon 2018 — Evan Dossey
Cat People (1942) — Aly Caviness
The Child's Play Series — Salem
The Shining (1980) — Dave Gutierrez
Hellroller — Richard Propes
Poltergeist III — Greg Lindberg
Scream — Heather Knight
The Witch — Rick Dossey
The Frankenstein Cycle — Lou Harry
A Nightmare on Elm Street 3: Dream Warriors — Sam Watermeier
Eastern Horrors — Alex Holmes
Unfriended — Austin Lugar
Freaks (1932) — Alys Caviness-Gober
As Above, So Below — Jonathan Curole
The Beyond — Nick Rogers
The Dentist — Mitch Ringenberg
The Halloween Franchise — Evan Dossey
No Sleep October: A Nightmare on Elm Street 3: Dream Warriors
No Sleep October: A Chinese Ghost Story & Encounters of the Spooky Kind


Made with real buttermilk. Creamy Ranch Dressing, the most popular way to liven up salads, veggie platters and even grilled fish. Consider it America's favorite dressing! Find your nearest O'Charley's at: ocharleys.com. Made in USA.
Great for dipping. Enjoy our signature Honey Mustard Dressing. Just the right blend of sweet with a hint of spice. Makes salads better and chicken tenders tastier! Find your nearest O'Charley's at: ocharleys.com. Made in USA.
Great for dipping. Full honey mustard flavor that's a guilt-free topping for salads and dip for chicken tenders. The lighter alternative to yum! 65% less fat than regular dressing. 50% fewer calories than regular dressing. This dressing: 6 g fat, 80 calories. Regular dressing: 17 g fat, 170 calories. Find your nearest O'Charley's at: ocharleys.com. Made in USA.
Brush our fat free Honey Mustard on grilled chicken, pork chops or bratwurst for a delicious glaze. In 1935, Henrietta Mahler started her own business by making small batches of her delicious homemade salad dressing right in her own kitchen. Using her signature recipes, she created a line of tangy sweet dressings that made her famous. Now, Henri's is bringing you the same homestyle goodness that Henrietta did over 70 years ago.