text:
The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor
F. Santos-Beneit, M. Barriuso-Iglesias, L. T. Fernandez-Martinez, M. Martinez-Castro, A. Sola-Landa, A. Rodriguez-Garcia, J. F. Martin
The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.
https://doi.org/doi:10.1128/AEM.00465-11
doi:10.1128/AEM.00465-11
The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP.pdfOther version, 1.22 MBLicence: Unspecified
http://dx.doi.org/10.1128/AEM.00465-11
Fingerprint Dive into the research topics of 'The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor'. Together they form a unique fingerprint.
Streptomyces coelicolor Medicine & Life Sciences
biosynthesis Earth & Environmental Sciences
DNA-Directed RNA Polymerases Medicine & Life Sciences
RNA Earth & Environmental Sciences
DNA-directed RNA polymerase Agriculture & Biology
antibiotics Earth & Environmental Sciences
gene Earth & Environmental Sciences
Anti-Bacterial Agents Medicine & Life Sciences
Santos-Beneit, F., Barriuso-Iglesias, M., Fernandez-Martinez, L. T., Martinez-Castro, M., Sola-Landa, A., Rodriguez-Garcia, A., & Martin, J. F. (2011). The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. Applied and Environmental Microbiology, 77(21), 7586-7594. https://doi.org/doi:10.1128/AEM.00465-11
Santos-Beneit, F. ; Barriuso-Iglesias, M. ; Fernandez-Martinez, L. T. ; Martinez-Castro, M. ; Sola-Landa, A. ; Rodriguez-Garcia, A. ; Martin, J. F. / The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 21. pp. 7586-7594.
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title = "The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor",
abstract = "The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.",
author = "F. Santos-Beneit and M. Barriuso-Iglesias and Fernandez-Martinez, {L. T.} and M. Martinez-Castro and A. Sola-Landa and A. Rodriguez-Garcia and Martin, {J. F.}",
doi = "doi:10.1128/AEM.00465-11",
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Santos-Beneit, F, Barriuso-Iglesias, M, Fernandez-Martinez, LT, Martinez-Castro, M, Sola-Landa, A, Rodriguez-Garcia, A & Martin, JF 2011, 'The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor', Applied and Environmental Microbiology, vol. 77, no. 21, pp. 7586-7594. https://doi.org/doi:10.1128/AEM.00465-11
The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. / Santos-Beneit, F.; Barriuso-Iglesias, M.; Fernandez-Martinez, L. T.; Martinez-Castro, M.; Sola-Landa, A.; Rodriguez-Garcia, A.; Martin, J. F.
In: Applied and Environmental Microbiology, Vol. 77, No. 21, 2011, p. 7586-7594.
T1 - The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor
AU - Santos-Beneit, F.
AU - Barriuso-Iglesias, M.
AU - Fernandez-Martinez, L. T.
AU - Martinez-Castro, M.
AU - Sola-Landa, A.
AU - Rodriguez-Garcia, A.
AU - Martin, J. F.
N2 - The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.
AB - The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α2ββ′ core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.
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DO - doi:10.1128/AEM.00465-11
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
Santos-Beneit F, Barriuso-Iglesias M, Fernandez-Martinez LT, Martinez-Castro M, Sola-Landa A, Rodriguez-Garcia A et al. The RNA Polymerase Omega Factor RpoZ Is Regulated by PhoP and Has an Important Role in Antibiotic Biosynthesis and Morphological Differentiation in Streptomyces coelicolor. Applied and Environmental Microbiology. 2011;77(21):7586-7594. https://doi.org/doi:10.1128/AEM.00465-11

redpajama_set_name: RedPajamaCommonCrawl


text:
SPEED-DATING
By Admin	| October 2, 2010
"Speed-Dating" tells the tale of three bachelors, Too Cool (Wesley Jonathan), Beaver (Leonard Robinson) and Dog (Chico Benymon), who spend their evenings putting on fake "speed dating" events in a effort to get large quantities of women together that they can then pick-and-choose from (while also making some money off the unwitting male and female participants). Too Cool is the main player of the group, easily wooing the ladies back to the bedroom while Dog is your typical chauvinist pig and Beaver is… well, Beaver may or may not be gay. Everything is working out fine, until the health inspector (an overweight, body-painted-blue Chris Elliott) denies their club a liquor license unless it is brought up to code, and the guys start to find themselves falling for a few of their conquests. Will the men save their club? Will they find love? Why is Chris Elliott blue, and is that Clint Howard scamming on chicks?
"Speed-Dating" is a funny movie, along the lines of New Zealand's romantic comedy "Sione's Wedding." While not all of the bits seem to connect perfectly (there is definitely the feeling of some scenes existing solely for the jokes contained within them), at least the narrative slides make you laugh. And, you know, if you make a comedy, and you make people laugh, haven't you succeeded?
I wasn't 100% onboard with the film's score, which dips into "Home Alone" territory with some of the music cues, and some of the acting from the non-leads can be pretty painful to watch, though the film does establish an over-the-top style early on that makes the lesser talented actors seem less like they're trying too hard. I mean, it's tricky to decipher: is an acting performance poor because the actor is going for over-the-top, or is everyone else just doing over-the-top better?
Overall though, again, "Speed-Dating" is a comedy that is actually funny, and that should be valued highly. While it does dip into the dramatic in a couple moments, it doesn't misplay its intentions and become melodramatic or tiresome. It could be a little tighter, but the film doesn't drag and it's a fun watch.
WORM FREE SOCIETY
Norman (Timothy Goff) is a little uneasy about his current job. Working opposite shifts with a sickly old man (David Weindel), and sharing the same desk,...
Roommate Wanted
Roommate Wanted, written and directed by Michael McCartney, is about a killer roommate that aims to make you laugh and shudder equally. The film blends...
"HELLBENT" FOR HOLLYWOOD
On October 20th at the Egyptian Theatre in Los Angeles, the American Cinematheque presents a screening of HELLBENT, a new "homo" horror comedy directed and...


It's a new year and you're looking for a professional cleaning service to help you keep your business in tip top shape. But, what types of cleaning services should you look for? What should you expect from the commercial cleaning service you hire?
At the top of the list is, of course, a skilled, trustworthy, professional cleaning staff. A great crew to carry out the actual cleaning and organizing tasks is the most important. You also want them to have OSHA compliance when necessary for particular cleaning projects.
From here, you want to think about your cleaning needs now and in the future. If you have a smaller office space with light traffic throughout the day, consider a cleaning plan that covers basic tasks on a regular or short term basis. Perhaps once a week the cleaning crew will come through and clean bathrooms, kitchenettes and general office areas, plus trash collection and weekly vacuuming.
If you are in a larger office space with retail, client spaces, or back offices with behind the scenes staff areas, you should consider a commercial cleaning service that offers full, in-depth cleaning for your business. One that can offer more beyond the basics and expand to your needs. Consider a cleaning crew that will also tackle areas like carpet cleaning, cubicle and partition cleaning, and window cleaning. You will probably have a greater amount of traffic to consider in this type of space. You'll want a cleaning group to sanitize and tidy up on a regular basis and more often than for a smaller office.
It's important to evaluate your needs when considering your office or retail business cleaning. These are just some of the many areas we can keep looking good for your business. Plus, making your facility look great every day is easiest when you hire a professional cleaning crew with special training on how to provide the right cleaning for your industry.
We would be delighted to care for your entire office building – big or small! Learn more about our commercial cleaning services and what we can offer you on a regular or one time basis. Our professional cleaning staff is bonded, insured, and works to maintain your OSHA compliance as well as follow any confidentiality agreement protocols required. Please contact us online or call us at 571.384.3415 for answers to your questions, evaluate your cleaning needs, and schedule your janitorial cleaning appointment today!


Textile Wrapped Stones – Inspired by the Japanese tradition of cane wrapping stones. Create a work of art that will bring peace and serenity to your home.
Mandala Painted Stones – Mandala making is an ancient art form done in a circular pattern. We will use acrylic paint on stones to create beautiful colorful designs.
In addition to the above options, participants will also be able to freestyle their own design on a garden stone, as well!
No pre-registration required; pay at the door when you arrive. Come between 5:00 and 6:30 to ensure completion of project.


As arts coverage fades, how will local arts organizations attract audiences?
After having launched my career as an arts reporter at The Ann Arbor News in 2004, I had become, over the course of nearly 12 years, the only long-term staff entertainment writer left standing in Ann Arbor when MLive Media Group announced a round of layoffs on January 6, 2016.
This time, though, my name was on the list, too.
Beyond my own personal circumstances, the layoff meant that artists/local arts organizations would take yet another hit in regard to press coverage, and thus find it even harder to share their triumphs, struggles, ambitions, and events with the community. Yes, the rise of social media has made it possible for arts organizations to be in regular contact with their subscriber list – but how, without minimal press coverage, will they now reach beyond that?
The region's biggest and oldest arts presenter, University Musical Society (UMS), appeared to see the writing on the wall way back in 2009, when the daily Ann Arbor News newspaper shut its doors (and the more online-oriented AnnArbor.com rose in its wake), and out-going arts reporters at the Detroit newspapers were no longer being replaced. In February 2010, UMS launched a website called UMS Lobby, where visitors can contribute their own responses to shows, or read, listen to, and watch bonus material about visiting artists on UMS' roster.
Up until that time, UMS could count on an Ann Arbor News preview and review of every event on their calendar, but as that practice gradually fell by the wayside, UMS started producing more of its own content for UMS Lobby – either internally, or by hiring it out.
The drawback of UMS Lobby, though, is that it lives apart from UMS' main website, so UMS is now in the process of a re-design – targeted for a mid-April release – that will pull content from the Lobby site and integrate it onto the UMS site.
Billmann noted that they've recently poured more of UMS' promotional budget into public radio and inserts that arrive between sections of The New York Times, both of which have proven effective.
"I'm not spending money with news sites that don't support the arts," said Billmann.
The underlying problem, of course, is that the online traffic numbers that appear too insignificant, from a business perspective, to a modern news organization are nonetheless highly significant and meaningful to the arts organization being covered.
Indeed, The Odd Couple has sold extremely well despite the fact that Northville's Tipping Point Theatre is producing the female version of the classic play simultaneously. Instead of grumbling and hand-wringing, the Rose and Tipping Point did what more and more marquee arts organizations are starting to do: collaborate.
According to Doral, they came up with a cross-promotion, whereby those patrons who saw both productions would be entered into a drawing for prizes like tickets or concession discounts. "We've gotten a great response," said Doral.
But like many arts organizations these days, the Rose has to strategically tailor its marketing plan to each project. When it took on an ambitious, large-cast world premiere zombie drama by Michael Brian Odgen, titled 2AZ, the Rose sought out underground periodicals and college newspapers, and set up a table at Comic Con, hoping to tap into The Walking Dead crowd. Unfortunately, this "outside the box" marketing approach didn't attract enough ticket buyers, and the (generally well-received) show closed 5 weeks ahead of schedule – so while the Rose remains staunchly dedicated to "add to the canon of American theater" (Doral's words) by producing new work, risk is simply part of the business.
Doral notes that a grant the Rose received in support of a 25th anniversary script preview tour –wherein actors perform scenes shows in different parts of Southeast Michigan and answer questions, alongside longtime artistic director Guy Sanville – has paid off in big ways. "We're seeing how many of those people are turning into ticket buyers," said Doral.
But this large-scale, special community outreach event isn't something the Rose can do each year, so they're also putting more time and effort into video. This can be a tricky venture, since some properties (like "The Odd Couple") restrict the use of any dialogue for promotional purposes, and the actors' Equity Union have their own rules about how much can be filmed. So the process often feels like a puzzle that needs solving.
"Right when we post them, though, we get lots of shares and comments," said Doral.
The Ann Arbor Symphony Orchestra, meanwhile, has beefed up its social media presence; strengthened its relationship with Detroit Public Television and classical music radio station WRCJ FM 90.9; prioritized small group performances at the Ann Arbor's Farmer's Market; and written their own previews and profiles to send out in e-newsletters.
The bulk of this effort falls to A2SO marketing director Emily Fromm, who started her job just as The Ann Arbor News broke ties with longtime entertainment free-lancers (like fine arts writer Susan Isaacs Nisbett).
To counter the press coverage gap, A2SO has increased its focus on community outreach programs, and designed programming that targets different age groups. KinderConcerts – short, 30 minute concerts at local libraries – have provided a good point of entry for many families.
Fromm's approach, in broad terms, is to make the orchestra feel more accessible. For instance Fromm will ask musicians to don party hats and eat cupcakes for a pre-rehearsal photo meant to drum up interest in A2SO's annual Mozart Birthday Bash concert. "I'd love to start a blog, but I also love to sleep," said Fromm.
Kerianne Tupac, marketing and communications director for University Productions at U-M's School of Music, Theatre and Dance, said that her department is focusing more advertising dollars in print monthlies and radio, and investing more time and money in social media (including the hiring of a videographer).
Tupac mourns the loss of press arts coverage not just from a marketing standpoint, but also because reviews offered U-M's performers-in-training with valuable feedback, while providing a space, too, for the community to have a performance-sparked conversation.
The Ark's marketing director, Barb Chaffer Authier, also says social media now plays a crucial role in communicating with the community, while paid advertising's impact has shrunk. "Putting more resources into paid advertising doesn't have the impact on ticket sales that actually engaging people in thinking and talking about the events does," said Authier.
Even so, Authier knows that established news sites draw a lot of visitors on a daily basis, so even if a site's arts coverage is scant, the promise of all those "views" is pretty hard to dismiss.


text:
Home Europe Rafale Jets On A Roll; Dassault Aviation 'On Verge' Of Outflanking LM's...
Rafale Jets On A Roll; Dassault Aviation 'On Verge' Of Outflanking LM's F-35 Jets For Swiss Air Force Deal — Reports
Anupama Ghosh
The French Rafale jets are roaring and soaring! After inking deals with India, Egypt, Croatia, and Greece, French firm Dassault Aviation looks set to sign a contract with the Swiss Air Force for Rafale fighter jets over F-35 stealth aircraft, as per reports.
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According to Asia Times, the Swiss Air Force launched the 'Air 2030' tender last year to acquire new fighter jets. The country is expected to buy 30-40 jets at an estimated cost of $7 billion by 2025.
Switzerland intends to use the jets in air policing missions, and the new jets will be inducted in the place of McDonnell Douglas F/A -18C/D Hornets and Northrop F-5E/F Tiger II antiques, which the country is currently using.
Apart from the Dassault Rafale, three other contenders in the fray are — the Lockheed Martin F-35, the Boeing F/A -18 Super Hornet and the Eurofighter Typhoon.
A twin-jet fighter aircraft, the Rafale can be used both from an aircraft carrier and a land base. It can be used in combat missions like air defense, making in-depth air strikes, aerial scouting missions, anti-ship strikes and nuclear deterrence.
It was introduced to the service of the French Navy in 2004 and that of the French Air force in 2006.
The Swiss Interest in Rafale Jets
The Asia Times report suggests the cost of the American jets may have come as a major hurdle for their acquisition.
Reportedly, Swiss politicians had initially favored buying the Boeing F-18 and the Lockheed Martin F-35 but later changed their minds. Leader of the Socialist group Roger Nordmann was quoted in the report as saying, "Buying the American F-35s, which are the most expensive, is excluded."
The Federal Office of Armaments or the Amasuisse, procured air surveillance system Sky View from the French firm Thales. It is believed that the Swiss may go ahead with consistency in their surveillance systems and aircraft.
Thus after the French firm Thales, French firm Dassault Aviation may be favored. Also, Priska Seiler Graf, a member of the Security Policy Commission, which is expected to have the final say on the decision of the aircraft, had only mentioned Rafale.
Before Switzerland, Croatia, India, Greece, and Egypt had signed deals with Dassault Aviation.
India's Purchase
The Rafale jets are a major defense acquisition for India in over two decades since the country acquired the Russian Sukhoi jets. The aircraft are being delivered in batches after an inter-governmental agreement was signed between India and France for the purchase of 36 Rafale fighters for 7.87 billion euros (Rs 59,000 crore) in 2016.
Dassault Rafale – Wikipedia
In September 2020, the first batch of five Rafale jets was inducted into the Indian Air Force (IAF). So far, IAF has received 23 aircraft from Dassault and the full delivery of 36 jets is expected to be completed by April 2022, as Defense Minister Rajnath Singh announced.
#RafaleInduction
IAF has formally inducted the #Rafale aircraft in 17 Squadron 'Golden Arrows' today, at Air Force Station, Ambala. The ceremony also marks #Rafale's full operational entry into IAF.
Glimpses of the Rafale in action with IAF. pic.twitter.com/WfohU5vMET
— Indian Air Force (@IAF_MCC) September 10, 2020
The first Rafale squadron has been established at the Ambala air force station in the northern Indian state of Punjab, while the second base will be at Hasimara base in West Bengal, close to the China border in the eastern sector.
The procurement of the Rafale jets did not come as a surprise, as IAF fleet was in need of urgent reinforcements. As reported by The Eurasian Times earlier, the nuclear capability of the Rafale had been a major reason for India to choose the French jets.
The report mentioned that the Rafale is considered similar to the Eurofighter Typhoon, the European fighter jet. The Eurofighter Typhoon is jointly manufactured by Germany, Spain, Britain, and Italy, which meant multiple clearances.
In the end, the Indian government chose to acquire the Rafale jets from France. Its capability to serve as an omni-role fighter, and nuclear-deterrence missions made Rafale favorable to India.
The Egyptian Deal
In May, Egypt confirmed its order of 30 Rafale jets. This order follows the purchase of 24 Rafale jets by Egypt in 2015. The latest deal is worth $4.8 billion and will be funded through a 10-year loan. Speaking about the contract, the French Defense Minister Florence Parly was quoted as saying the deal "reinforces the strategic and military partnership between France and Egypt".
After Saudi Arabia and India, Egypt is the world's third-largest arms importer, according to the Stockholm International Peace Research Institute.
Indonesia & Croatia
Indonesia is also set to acquire the Rafale jets once the country's President puts his stamp of approval. Recently, the country turned down an offer to acquire F-16 jets from the US, after the US denied selling the F-35 fighters to the Southeast Asian country. According to reports, Indonesia plans to buy 36 Rafale jets.
Croatia is acquiring Rafales as part of its defense modernization plan. The country is in the process of adding new as well as second-hand aircraft to replace the Soviet-era aircraft in its inventory.
The Eurasian Times reported earlier that for its Multi-Role Fighter Aircraft program, Croatia issued tenders and in May 2021 announced the selection of the Dassault Rafale F3-R. As part of the acquisition, Croatia will receive 12 second-hand aircraft for $1.2 billion. The first six aircraft will be received by Croatia in 2024, and the rest in 2025.
Last year, France had approved the sale of 18 Rafale fighters to Greece in a deal worth €1.7 billion. Greece will receive 12 second-hand and six new Rafales and the first aircraft will be received in 2021, The Eurasian Times reported.
The Rafale fighter jets will replace the aging fleet of Mirage 2000 in the HAF. The Dassault fighters will be equipped with Meteor air-to-air missiles along with re-receipts, or even purchases of new pieces from existing weapons such as Exocet, Scalp, MICA, said a Greek media report.
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Anupama has been trained as a historian but has an immense interest in international relations. After completing her Ph.D. from the Delhi University, she has briefly worked as an Associate Professor in a Delhi-University College. Since 2016, she has been associated with Kochi-based think-tank CPPR in various capacities. She has also contributed articles in Indian digital platforms and is a regular contributor to an Istanbul-based think-tank. CONTACT: anupama86ghosh@gmail.com


Taps and dies are tools for cutting threads. A tap is a bolt-like tool for making female threads. A die is a nut-like tool for making male threads. Taps and dies can make many "impossible" repairs possible, even easy.
Both tools have threads, a little like the threads on nuts and bolts, but the threads on taps and dies have cutting edges to cut threads in smooth metal. A tap screws into a smooth hole and makes it into a threaded hole; a die puts threads onto a formerly smooth shaft.
Taps and dies can be bought one at a time in any good hardware store, and are not at all expensive. You can buy a small set of commonly used sizes for less than $10.
Taps come in three types: plug, taper, and bottoming. Plug taps are the most commonly seen. A plug tap has a fairly steep taper at its point, so that it will cut full depth threads after only a few turns. This is the most efficient type for production use.
Bottoming taps have no taper at all. As a result, bottoming taps cannot be used to start a new thread. Bottoming taps are used only to bring an existing thread down to the bottom of a "blind" hole (a hole that does not go all the way through the material). I have never found any need for a bottoming tap on a bicycle.
Taper taps are the best type to buy for bicycle use. Taper taps are like plug taps, except that they have a more gradual taper to the point. The gradual taper makes it easier to tap a straight hole by hand, although this can also be done with a plug tap if you have a good eye. Large tap sets usually come with all three types of each size, so you wind up with three times as many taps as you need if you buy a large set!
Taps are metal-cutting tools, and therefore need to have very hard cutting edges. Because of the type of steel and heat treatment required to enable them to cut metal, they are extremely brittle. Considerable care is needed to use taps without breaking them. This is especially so in the case of the smaller size. If you break a tap off in a hole, it can be very difficult or even impossible to remove the broken piece! A broken tap cannot usually even be drilled out, because the tap is as hard as or harder than the drill bit.
Taps are usually broken by bending, not twisting.
It is possible, if you are very, very careful, to use a tap by turning it with a conventional adjustable wrench, but it is very risky. A wrench that pushes only on one side puts an asymmetrical stress on the tap, and is very likely to break it. Instead, you should buy a proper tap wrench. This will have a "T" handle, making it easier to turn the tap without bending it.
As the tap cuts, it leaves a chip of metal that has been cut away. The tap has two through four "flutes" (grooves) to allow the cutaway chips to escape from the hole. If the flutes become clogged with chips, the tap will bind. It will break if forced.
The chips tend to be formed in long strips. Good tapping technique requires that you break up these strips into smaller pieces that can escape along the flutes. This is done by turning the tap backward.
The procedure is to turn the tap forward one-third to one-half turn, or until you feel increased resistance. Then turn it backward until you feel it turning more easily one-quarter to one-half turn, typically. For deep tapping, it will usually be necessary to go partway in, then unscrew the tap entirely so that you can clean the chips out before continuing.
Generally, you should use some sort of cutting fluid with taps. There are special cutting oils made for the purpose, but these are not usually needed except when power tools are used. For general hand tapping of steel, any convenient oil will do nicely; for aluminum, kerosene is best.
I keep a couple of containers on my workbench, one filled with used motor oil, one with kerosene. I just dip the tap into the appropriate can before I ,start to cut threads with it. I use the old motor oil because I have it on hand, and because it doesn't matter if I leave chips in it from the tapping, since that oil is used only for cutting, not lubrication.
It is essential that you drill the correct size hole for the size of tap you are using. If the hole is too small, you are very likely to break the tap when you try to cut the threads. If the hole is oversize, the tap will go through easily. but the threads will be shallow and easy to strip. When you buy taps, you should also buy drill bits to go with them. These bits should be kept with the taps, and used only for drilling holes that are to be tapped.
Drill bits come in four size systems: fractional, number, letter, and metric sizes. Most small home workshops will already have a set of fractional size bits, typically from 1/16-inch to 1/4-inch in 1/64-inch increments. This type of set will work after a fashion, but if this is all you have, you will not have the exact correct size for most size taps. The most popular tap sizes require a number-size drill bit to make the proper-sized hole. For instance, a #10-32 tap (the size designation is explained in the accompanying story) requires a #21 drill bit for proper fit. This is in between 5/32-inch and 11/64-inch. If you used a 5/32-inch bit, you would be very likely to break your tap. If you used an 11/64-inch bit, the tap would go through the hole okay but the threads would be shallow and easily stripped.
A set of number-size drill bits includes 80 bits, ranging in size from .0135 inch to .2280 inch. These essentially overlap the basic 1/64-inch to 1/4-inch fractional set that you may already own. Most of these number-size drill bits will be of no use to you, and you'll spend a lot of money to get 80 of them. It makes more sense to spend a few dollars to buy the few sizes you really need.
If you are a high roller, you can spend a few hundred dollars on a good tap and die set, but 95 percent of the tools in such a set will be of no use for bicycle work. Instead, it is better to buy just the particular taps and dies that you have a use for.
While I do not recommend the large. expensive tap and die sets with three taps of every size and dies to go with them, there is one small and inexpensive type of tap set that makes plenty of sense for the small workshop. This type of tap set is sometimes called an "electrician's" set. It will typically have taps only, no dies. There will usually be six sizes ranging from #4-40 to 1/4-20. The best thing about this type of set is that it includes the appropriate drill bits to go with the taps, all conveniently packaged with a tap wrench.
There are two threading systems in common use in this country: SAE (American) and ISO (metric).
SAE threads are described in the form (diameter)-(threads per inch) -- for instance: 1/4-20 means that the bolt is 1/4-inch in diameter and has 20 threads per inch. Diameters smaller than 1/4-inch are specified by a number size. For example, #10-32 is a very common size; the "#10" is equivalent to 3/16-inch and there are 32 threads per inch.
Metric threads are specified in the form (diameter)-(millimeters per thread). For example, 5 mm-.8 means that the diameter of the bolt is 5 millimeters and that the threads are .8 millimeters apart. ISO metric threads are standardized to the extent that the thread pitch is not usually mentioned, unless it is non-standard for the diameter for example, 5 mm-.8 is often just called 5 mm, but if the thread pitch is other than .8, it has to be specified. Likewise, 6 mm-l.0 is a standard ISO size, and can be referred to as "6 mm."
A few sizes are more or less interchangeable between the American and metric systems; particularly useful is #10-32/5 mm. These can generally be interchanged with no problem. This is a common size on bicycles. The eyelets for attaching racks and mudguards to the dropouts are 5-mm-threaded on most of the better-quality bicycles; this size is also used for toe-clip bolts, and various brake and derailer parts. Since 5 mm and #10-32 are essentially the same size, you could buy either size tap, but the American #10-32 size is easier to find and likely to be much cheaper.
How and Where Do You Use Taps and Dies on a Bicycle?
Taps and dies are used only occasionally in routine bicycle repair, but they have some specialized applications that can be very handy.
Older bicycle mechanics remember when almost every new European bike needed its pedal threads cleaned out with a tap before the pedals could be installed. Chrome plating fouled up the threads' dimensional tolerances, and it fell on the mechanic's shoulders to fix the problem. Today's aluminum cranksets and better manufacturing techniques have solved the problem, and the main use for pedal taps is to convert French-threaded cranks to the more commonly available English pedal thread size (9/16 x 20). Any work on pedal threads requires special taps, sold through bicycle tool sources, because you need a left-hand thread tap for the left crank.
When a rear quick-release skewer breaks at the threads, you can use a die to recycle it, making a (shorter) front quick-release skewer. Skewers come in 5 X 0.8 and 5 x 0.9 threadings; measure yours with a thread pitch gauge.
Special taps are made for bottom bracket threads. These can clean up threads which have been lightly damaged, or have become clogged with paint or threadlock compound. Unless a bottom bracket shell with seriously-damaged threads is very thin, or already Italian-threaded, it can be resurrected by tapping it out to the slightly-larger Italian size. Cartridge bottom brackets with Italian threading are available from Phil Wood, Velo Orange. TA and perhaps other manufacturers. A bottom-bracket tap set includes a special tool to align the taps at each side of the bottom-bracket shell with one another, and a cutter to smooth and align the cup/ lockring face at each end of the shell.
But the biggest use of taps and dies isn't repairs, it's making your own accessories. If you can cut threads and bend and pound metal rods, you can make quite professional-looking racks, custom-designed to your needs and wishes. You can make special brackets to put lights on pannier racks, better supports for handlebar and seat bags, etc.
Here's a simple example: I wanted my Pletscher rear carrier to be a bit more rigid. So I drilled out the rivets that hold the legs to the platform and replaced them with 1/4-inch bolts. I drilled 1/4-inch clearance holes through the legs, then used a #7 drill and a 1/4 - 20 tap on the holes in the platform. I bolted it together, putting locknuts on the inside. (The locknut makes the assembly quite vibration-resistant.) This job was not much trouble, and it resulted in a noticeable improvement.


text:
Stand with Kashmir
Surveillance State on Speed: Now India Plans to Issue Unique IDs to Kashmiri Families
What is the JK Family ID? In a recent move, Manoj Sinha, the Lieutenant Governor of the indian regime in Kashmir announced that the government will be building an "authentic database" of Kashmiri families. He said that this will be achieved through assigning unique alphanumeric IDs to each and every family of Jammu and Kashmir, which…
Fests: Stealthy Normalization of indian Occupation in Kashmir
December 06, 2022. indian-occupied Kashmir. The indian Occupation in Kashmir has always been at war with the local population, to maintain forcible control of the territory. However, this war is not always waged through the gun or through brute power and war crimes. Stealthy "normalization" has also often been the weapon of choice for india against Kashmir's freedom movement. But what is "Normalization" and how is india using it as another tactic to suppress Kashmiris?
india is Colonizing Kashmir's Economy
November 23, 2022. indian-occupied Kashmir. "Economic Development" was the buzz word as the indian Home Minister moved to abrogate Article 370 and with it, the semi-autonomous status of Kashmir. However, the ground reality since that day itself and in its long-term aftermath has been different. Small businesses have collapsed, and the fruit industry has been made to suffer immense losses. Most significantly: in its report for May — August 2022, the Centre for Monitoring indian Economy has pegged the unemployment rate in Jammu and Kashmir for the month of August at a staggering 32.75%, which is the highest in past 6 years. …
Under Barbed Wire: Religious Freedom in indian-Occupied Kashmir
October 04, 2022. indian-occupied Kashmir. Despite being currently ruled by a right-wing Hindu nationalist government, India claims to be a "secular democracy." Its constitution allows for the freedom of religion, meaning people should be able to follow and freely practice their religious beliefs. India's constitution says that the state will not "discriminate, patronise or meddle in the profession of any religion". However, when it comes to Islam and Muslims, and especially when it comes to its colony in Kashmir, religious freedom and claims of secularism mentioned in the Indian Constitution is completely absent.
Armed Forces Special Powers Act: A Colonial Law
September 19, 2022. indian-occupied Kashmir. The Armed Forces Special Powers Act or AFSPA is a draconian law enacted by the Indian parliament specifically to grant special powers to the Indian army operating in certain 'disturbed' areas. Although very brief in nature and comprising of only six sections, along with other special powers, the law comes with blanket impunity for the Indian army. This army comprises of 1.4 million active personnel, and is the second largest in the world. While this law was initially enacted in the year 1958 to deal with the Naga liberation movements in the Northeast, in 1990 it was extended to Kashmir at the…
From VDCs to VDGs — A Legalised Network of Armed Gangs in indian-occupied Kashmir
September 14, 2022. indian-occupied Kashmir. Aside from the Indian army and its paramilitary forces, as well as the Jammu and Kashmir police, India and its client regimes in Kashmir have always employed and trained 'civilian' networks to harass, intimidate, surveil, and kill Kashmiris and suppress the freedom movement. These government…
The Amarnath Yatra: uncovering india's militarized pilgrimage in indian-occupied Kashmir
August 25, 2022. indian-occupied Kashmir. The Amarnath Yatra is a holy Hindu annual pilgrimage taken in the second half of summer in Kashmir. The Yatra is one of the largest pilgrimages in the world with almost half a million Hindu devotees (yatris) traveling to the Amarnath cave to pay respects…
india is coercing Kashmiri academics to submit and self-censor
August 18, 2022. indian-occupied Kashmir. Since August 2019, India has intensified its attacks against all facets of Kashmiri civil society: human rights defenders and organizations, journalists, lawyers, and activists. More recently, the Indian government has escalated its crackdown on Kashmiri academics and researchers, in an attempt to completely stifle all forms of independent inquiry and documentation. Those who have been targeted are primarily involved in critical research on Kashmir and on India's long-term colonization of the region.
india's Independence Day Circus in indian-Occupied Kashmir
August 15, 2022. indian-occupied Kashmir. Every year, the indian government plans grand Independence Day celebrations in occupied Kashmir in an attempt to enforce its nationalism on the people and display false propaganda of Kashmiri's celebrating indian independence for indian and international audiences. Last year, amidst strict curbs on any kind on civilian movement, city centers were lit up in the colours of the indian flag. But this year, the settler-colonial state has already set an even more authoritarian style of celebrations into motion. …
Dear Masaba, Why Did You Film in Occupied Kashmir?
August 12, 2022. indian-occupied Kashmir. Netflix's Masaba Masaba released its 2nd season. While many are celebrating the representation of desi women the show provides, the fact that it is normalising india's occupation of Kashmir is not being spoken about, so we wrote an open letter to Masaba addressing her normalisation campaign. This letter does contain SPOILERS but not the kind you're thinking of.
SWK is a Kashmiri diaspora-led international solidarity movement that seeks to end the Indian occupation and support the right to self-determination.


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Target niche audiences across channels or leverage Umbel's Digital Media specialists to analyze data, build campaigns, and sell tickets across Facebook, Instagram, Twitter, Google and other channels.
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text:
Merle Oberon (egentlig Estelle Merle O'Brien Thompson; født 19. februar 1911, død 23. november 1979) var en indisk-født britisk skuespiller.

Biografi
Oberon opholdt sig i Indien indtil hendes 17 år, da hun flyttede til London. Hendes første år i London efterlod ingen store spor i filmhistorien, men hendes roller var stort set ubetydelige og let glemt.

I 1933 spillede hun først en rolle i filmen Henrik den Ottendes privatliv, hvor hun havde rollen som Anne Boleyn. I 1934 optrådte hun som Lady Marguerite Blakeney i Den røde Pimpernel overfor Leslie Howard og begyndte sin karriere i USA. Merle Oberon blev nomineret i 1935 til en Oscar for bedste kvindelige hovedrolle i filmen Den sorte engel, men var efter ikke bemærket nogen nogen tid efter. I slutningen af 1930'erne og 1940'erne var der en filmperiode med ca. 20 film.

Merle Oberon var gift fire gange - første gang med den britiske instruktør af Den Røde Pimpernel, Alexander Korda. Hendes sidste ægteskab var med skuespilleren Robert Wolders fra 1975 indtil sin død i 1979.

Hun er blevet tildelt en stjerne på Hollywood Walk of Fame på 6274 Hollywood Blvd.

Filmografi (udvalg)
 1933 – Henrik den Ottendes privatliv
 1934 – Den røde Pimpernel
 1935 – Den sorte engel
 1936 – De tre
 1937 – I, Claudius (ufærdig)
 1938 – Ham og ingen anden
 1938 – Lady X's skilsmisse
 1939 – Stormfulde højder
 1940 – 'Til We Meet Again
 1941 – Affectionately Yours
 1941 – Gennemtræk i paradis
 1943 – Vi mødes på Broadway
 1943 – I dag og for evigt
 1944 – Den mystiske logerende
 1947 – Nattens melodi
 1948 – Berliner-ekspressen

Kildehenvisninger

Eksterne henvisninger

Skuespillere fra Indien
Skuespillere fra Storbritannien
Personer fra Mumbai
Hollywood Walk of Fame
Briter i 1900-tallet

redpajama_set_name: RedPajamaWikipedia


text:
define('initService', ['adminApp'], function (adminApp) {

  adminApp.service('InitService', ['$http', function ($http){
    this.config = function () {
      return $http({method: 'GET', url: '/config.json'});
    };
  }]);
  adminApp.service('logging', function(){
    this.info = function(args) {
      console.log(args);
    };

    this.warn = function(args) {
      console.warn(args);
    };

    this.err = function(args) {
      console.err(args);
    };
  });
  adminApp.service('socket', [ '$http', 'logging', function ($http) {
    $http.defaults.useXDomain = true;
    // var socket = new WebSocket('ws://localhost:8080/solutions/point');
    // var self = this;
    // var temporyList = [];
    // socket.onopen = function (){

    //   self.connectSuccess = true;
    //   angular.forEach(temporyList, function(value){
    //     socket.send(value);
    //   });
    //   temporyList.length = 0;
    // }

    // socket.onmessage = function(event){
    //   logging.info(event.data);
    // }

    // $http({
    //     url: 'http://localhost:8080/solutions/record',
    //     method: 'GET'
    //   }).then(function (data){
    //     console.log("====>", data);
    //   }).then(function (err){
    //     console.log(err);
    //   });

    this.send = function () {
      // if(self.connectSuccess){
      //   socket.send(data);
      // } else {
      //   temporyList.push(data);
      //   logging.warn("Web Socket is connecting ... ");
      // }
    };

  }]);

});

redpajama_set_name: RedPajamaGithub


text:
BC Born: Mathieu Caron
By Sheldon Lee
Mathieu Caron's destiny could very well have been laid out before him long before his ability to even form memories had developed. The Chilliwack Chiefs goaltender says he got started between the pipes around the age of six months old.
"As the story goes, I was at the babysitter's, who had some younger kids as well," said Caron. "They would place me in front the mini-stick hockey net and just start shooting at me."
Caron would wind up putting skates on for the first time between the ages of three and four and instantly found his calling.
"I loved it," he said. "It was just something that came naturally to me, so I started playing for fun."
Enter Caron's dad Rene, who would go on to become the coach of Mathieu's novice hockey team.
"There were times when we couldn't find a player to play net, so my dad would just throw me in there because I didn't mind," said Mathieu. "Nobody forced me, that's for sure. I just found myself most comfortable in net."
Standing in front of a rock-hard rubber disc doesn't exactly appeal to the masses, no matter what age you are. Then again, that's exactly what makes goaltenders special.
As time went on, Caron continued to play through the Abbotsford minor hockey ranks. The thought of joining his father, a teacher at Yale Academy, was always an intriguing one, but it wasn't until grade nine that it would become a reality.
Caron's first season with Yale proved to be a valuable one. During his first year with the U15 team, he wound up getting called up to the U18 team because of injuries. He would get five regular-season games under his belt with the older club that year, all of them wins.
Yale Academy has produced multiple established and soon-to-be established NHLers. Diehard Vancouver Canucks fans will know that Jake Virtanen laced up the skates for Yale Secondary back in the day.
Caron mentioned he was lucky enough to rub shoulder with a other few top-calibre players himself.
"My U18 year, I got the chance to play with Bowen Byram, Dylan Cozens and a couple others making their NHL debuts [this year]," he said. "It was a fun experience for us to be able to fly around and bond as a team, play in different tournaments and get to see the continent a little bit."
While Caron was in his final year at Yale, the Chilliwack Chiefs came knocking and named him an affiliate player. By doing so, Caron had the ability to play for both his registered club and the Chiefs. Looking back, the timing worked out quite well for him.
"I got a front-row seat to a memorable playoff run that year," he said. "In the four years since, I feel like I've seen everything there is to see in junior hockey."
Everything, including an RBC Cup National Championship in 2018.
The Chiefs entered that tournament as the host city and, after losing their first game to their BCHL counterparts the Wenatchee Wild, swept the remaining five contests, including a 4-2 final game victory over the Wellington Dukes of the Ontario Junior Hockey League.
It was a grind of a season and Caron feels he significantly benefitted from it.
"From the get-go, Chilliwack's been a winning organization," he said. "I think everyone knows that. I saw during that run what it really takes to be a championship team and how much work it really involves. Some teams don't really know the feeling. That's what I take away the most. I know what it takes to win."
Chiefs head coach Brian Maloney tends to agree. Maloney took over as head coach right before that RBC Cup run and has had Caron as his goalie every since.
"He's a kid that always listened well, studied the game really well and never hesitated to ask questions," said Maloney. "He's just a phenomenal teammate. Usually, when you do all those sorts of things, your game begins to develop, and because of that, he's one of the best goalies in the league."
In February of 2020 the Chiefs announced Caron had committed to Brown University – a school with rich academic tradition and one of the oldest hockey programs in the NCAA. So, what does it mean to him to dawn the jersey of such a coveted institution?
"The big goal for me out of junior hockey was to get that scholarship in the States and continue playing hockey," said Caron. "I'm not sure it's fully sunk in yet that I'll be going there next year, just because it's been so long wearing a Chiefs uniform. I really couldn't be more excited to get down there and getting my chance to play."
Brown is an Ivy League school and is known for its academic prowess. That is no coincidence as Caron and his family have always put an emphasis on education.
"My parents always pushed me to do well in school, so that was a big focus of mine growing up," he said. "When I get to Brown, I've set myself on getting into the medical side of things. The academics at that school are very well known and I look forward to that challenge and that aspect of things."
In the meantime Caron is in his final year with the Chiefs, a team with some big business to take care of. Chilliwack is currently sitting atop their respective BCHL pod this season and are looking to finish atop the standings. It's not easy to sum up four fun-filled years spent on BCHL ice in a couple sentences, but Caron did his best.
"Practically being a hometown kid and being part of such a wonderful organization has been almost surreal," said Caron. " When you combine that with the fans and the community, I know that Chilliwack will always hold a special place in my heart. That's how much I love being here."
Tagged: BC Born, Brian Maloney, Chevrolet, Chilliwack Chiefs, Mathieu Caron
BCHL Podcast: Apr. 23 (Tyson Jugnauth, Kaeden Lane & Noah Serdachny)
BC Born: Tyson Dyck
NCAA Roundup: Dec. 9
League announces All-Star Fan Vote results


text:
export default {
  format: 'combined'
}


text:
package demo.tests;

import net.thucydides.core.annotations.Managed;
import net.thucydides.junit.runners.ThucydidesRunner;

import org.junit.Test;
import org.junit.runner.RunWith;
import org.openqa.selenium.By;
import org.openqa.selenium.WebDriver;
import org.openqa.selenium.WebElement;
import org.openqa.selenium.htmlunit.HtmlUnitDriver;

@RunWith(ThucydidesRunner.class)
public class GoogleThucydidesDriverIT {
	@Managed
	public WebDriver webdriver;

	@Test
	public void googleSearchTest() {
		// And now use this to visit Google
		webdriver.get("http://www.google.com");

		// Find the text input element by its name
		WebElement element = webdriver.findElement(By.name("q"));

		// Enter something to search for
		element.sendKeys("Cheese!");

		// Now submit the form. WebDriver will find the form for us from the element
		element.submit();

		// Check the title of the page
		System.out.println("Page title is: " + webdriver.getTitle());
	}

  public static void LegazyWebDriverTest(String[] args) {
    // Create a new instance of the html unit driver
    // Notice that the remainder of the code relies on the interface,
    // not the implementation.
    WebDriver driver = new HtmlUnitDriver();

    // And now use this to visit Google
    driver.get("http://www.google.com");

    // Find the text input element by its name
    WebElement element = driver.findElement(By.name("q"));

    // Enter something to search for
    element.sendKeys("Cheese!");

    // Now submit the form. WebDriver will find the form for us from the element
    element.submit();

    // Check the title of the page
    System.out.println("Page title is: " + driver.getTitle());

    driver.quit();
}
}


November is a busy month for holidays and early release days. Please remember that on early release days there is NO PRE-K EXTENDED DAY AVAILABLE, unless your child attends the later extended day program from 2:20-5:45. In the case that your child is scheduled for PRE-K EXTENDED DAY on a day that an early release day falls on, and you would like to swap that day for another within that same week, please let us know.
-Early Release Days:Thursday November 8th, Wednesday November 14th, Wednesday November 21st, and Thursday November 29th. NO PRE-K EXTENDED DAY on these days, unless otherwise arranged with Noelle Ahearn.
This month we will begin our letter of the week unit. For the first week we will be learning about the letter A and Animals. The following week we will be learning the letter B and studying our Bodies. During the short week of Thanksgiving we will be creating crafts for you and your family, along with "I am Thankful books." The last week of November brings us to the letter C and we will be discussing "creepy crawlers" and creating bug crafts.
-All other donations are greatly appreciated!


New Orleans, Louisiana sludge metal icons EYEHATEGOD are proud to announce their long-anticipated US tour beginning in June, following an appearances at this year's edition of the Maryland Deathfest. Sponsored by MetalSucks and SMN News, the 18-date trek begins at Blackbooth in Orlando, Florida on June 3 and will rage through 16 more cities before coming to a close in Chicago, Illinois at the Empty Bottle (where they play two nights). Here are all dates.
Support will come primarily from psychedelic black metallers Nachtmystium (from June 4 – 15) but will also feature a rotating roster of crushing openers throughout the tour including Brutal Truth, Withered, Black Anvil, Tombs, Howl, The Atlas Moth, Black Tusk and Strong Intention.
Since forming in 1988, EYEHATEGOD have defied all odds and continue to exist and persist despite personal and professional setbacks. They've been called doomcore, sludge and stoner rock, survived line-up shuffles, label hassles and a short-lived split. And after more than a decade of creating some of the most corrosive, vile music known to man, EYEHATEGOD still haven't lost the piss and vinegar that fueled them back in '88.


text:
// ----------------------------------------------------------------------------------
//
// Copyright Microsoft Corporation
// Licensed under the Apache License, Version 2.0 (the "License");
// you may not use this file except in compliance with the License.
// You may obtain a copy of the License at
// http://www.apache.org/licenses/LICENSE-2.0
// Unless required by applicable law or agreed to in writing, software
// distributed under the License is distributed on an "AS IS" BASIS,
// WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
// See the License for the specific language governing permissions and
// limitations under the License.
// ----------------------------------------------------------------------------------
namespace Microsoft.WindowsAzure.Management.HDInsight.Cmdlet.ServiceLocation
{
    /// <summary>
    ///     Provides services to fake out service location during debugging and test scenarios.
    /// </summary>
    internal interface IServiceLocationSimulationManager : IServiceLocationManager
    {
        /// <summary>
        ///     Gets or sets the mocking level to use when performing service location.
        /// </summary>
        ServiceLocationMockingLevel MockingLevel { get; set; }
    }
}


Keep your wooden furniture clean and scratch free.
All you need is an amazing solution that will get rid of the scratches. The ingredients required to make solution are, mix half cup of vinegar with half cup of olive oil to make a solution.
Use old pillow cases to clean your ceiling fan!
Here is a very easy way to clean your fans, if you have old pillow covers instead of throwing them away use it to clean your fan.


text:
What is GIST?
Patient Resource Toolkit
Understanding GIST
Types of GIST
GIST Diagnosis
Risk of Recurrence
Mutations & Mutational Testing
Treatments for GIST
Resistant GIST
Managing GIST
GIST Education
About LRG
The LRG Story
GIST Specialists Database
GIST Clinical Trials
Financial Aid for GIST Patients
GIST Mentor Program
Coping With GIST
LRG Science
LRG Digital Library
Home/Ignyta Announces Results for Potential New Cancer Treatment
Ignyta Announces Results for Potential New Cancer Treatment
By Pete Knox|2018-10-30T09:42:39-04:00July 12th, 2016|Clinical Trials, News, Research|
Dr. Alexander Drilon of Memorial Sloan Kettering Cancer Center recently reported at AACR on a potential new cancer treatment, Entrectinib, a receptor tyrosine kinase (RTK) inhibitor developed to target tumors carrying NTRK1, NTRK2, NTRK3, ROS1, or ALK gene rearrangements.
Entrectinib, an investigational therapy, is designed to target specific causes of certain types of cancer. The STARTRK-2 trial is recruiting patients with gene rearrangements in NTRK1, NTRK2, NTRK3, ROS1, or ALK. For more information about the Clinical Trial call 1-844-STARTRK. If you qualify for study enrollment, but there is not a site nearby, it may be possible to open a research center that could be closer to where you live or for Ignyta to support your travel expenses to a research center for STATRK-2 study visits.
Entrectinib Achieves High Response Rate Across Solid Tumors
Treatment with the novel multikinase inhibitor Entrectinib achieved objective responses in 79% of patients with solid tumors associated with NTRK, ROS-1, or ALK rearrangements, according to updated data reported at the 2016 AACR Annual Meeting.
Of 24 evaluable patients in the study, 19 had objective responses to Entrectinib. The agent also achieved durable responses in primary brain tumors and brain metastases, including one complete response.
A global phase II basket study of Entrectinib has begun and will involve patients with locally advanced or metastatic solid tumors with NTRK, ROS-1, or ALK-positive tumors, said Alexander Drilon, MD, an assistant attending physician at Memorial Sloan Kettering Cancer Center.
"We observed rapid [within 1 months of treatment initiation] and prolonged responses, including 11 of 13 patients with ROS1-rearranged non–small cell lung cancer and an additional patient with melanoma," Drilon said during a press conference. "The long ongoing response is approaching 2 years and 3 months."
Findings were reported from the phase I development program of Entrectinib, involving the STARTRK-1 and ALKA-372-001 trials. In the STARTRK-1 trial, Entrectinib was given continuously to 65 patients in the United States, Europe, and Asia. ALKA-372-001 involved 54 Italian patients treated with intermittent and continuous dosing. All 119 patients in both studies had tumors with NTRK/ROS1/ALK alterations.
The 19 patients whose tumors responded to Entrectinib represented diverse malignancies: non–small cell lung cancer (NSCLC), colorectal cancer, melanoma, gastrointestinal stromal tumor, and mammary analog secretory carcinoma (MASC). Patients with NTRK1/2/3, ROS1, and ALK gene fusions responded to the agent.
In a subgroup with ROS1-rearranged tumors and no prior exposure to a tyrosine kinase inhibitor (n = 14), treatment with Entrectinib led to objective responses in 12 patients (86%). Two patients had complete responses. Eleven of the responding patients had NSCLC and the other had melanoma. In the ALK-mutated group (n = 7), 57% of patients experienced a response. The majority of patients in this group had NSCLC (n = 5).
Entrectinib's activity in NSCLC was particularly noteworthy in that one patient had a partial extracranial response but a complete response in the brain. This patient had received 4 prior therapies, including a PD-1 inhibitor, and had a poor baseline performance status. The patient remains on Entrectinib and has been progression-free for >12 months, Drilon reported.
"This was noteworthy because the brain is a relatively common site for NSCLC metastasis and other therapeutics may not be as effective against brain metastases," he said.
In the subgroup of NTRK-rearranged cancers (n = 5), 100% of patients with various tumor histologies and fusion types responded to Entrectinib. The agent resulted in "dramatic intracranial activity" in all three patients with central nervous system disease (CNS).
"Thus far, Entrectinib is the only Trk inhibitor that has demonstrated CNS activity," said Drilon.
According to the abstract, the most common treatment-related adverse events at the 600 mg daily dose were fatigue/asthenia (47%), dysgeusia (32%), constipation (26%), dizziness (21%), paresthesia (21%), diarrhea (16%), myalgia (16%), and weight gain (16%).
"We do need to validate these results in larger numbers of patients, but patients and their physicians should strongly consider comprehensive genetic profiling to determine whether these gene fusions are present in the patient's tumors," said Drilon. "If they are, there is a good chance that treatment with a Trk inhibitor like Entrectinib will result in very meaningful benefit."
Patient accrual has already begun in the larger phase II STARTRK-2 trial, designed to confirm the results observed in the STARTRK-1 and ALKA-1 trials. The recommended phase II dose from the earlier studies was identified as 600 mg daily. The study is enrolling those with NTRK1/2/3, ROS-1, and ALK rearrangements (NCT02568267).
Entrectinib (RXDX-101) is an orally available, ATP-competitive tyrosine kinase inhibitor that has demonstrated low to subnanomolar efficacy against 5 kinases (TrkA, B, and C; ROS1 and ALK). The agent has exhibited in vitro and in vivo activity against NTRK1/2/3-, ROS1-, and ALK-rearranged cancers.
NTRK1/2/3, ROS1, and ALK occur in multiple types of cancer, especially some of the less common malignancies, such as glioblastoma, Spitz melanoma, cholangiocarcinomas, and papilloma thyroid cancer. No currently available cancer therapies address all of those mutations, Drilon noted.
Brain metastases occur in 20% to 40% of patients with cancer and are especially prevalent in lung and breast cancer and melanoma. Some types of primary brain tumors have associations with NTRK fusions, including astrocytoma, glioblastoma, and pediatric gliomas.
"An optimal therapy would address by systemic and central nervous system disease," said Drilon.
For more information about the Clinical Trial call 1-844-STARTRK. If you qualify for study enrollment, but there is not a site nearby, it may be possible to open a research center that could be closer to where you live or for Ignyta to support your travel expenses to a research center for STATRK-2 study visits.
Drilon A, De Braud FG, Siena S, et al. Entrectinib, an oral pan-Trk, ROS1, and ALK inhibitor in TKI-naïve patients with advanced solid tumors harboring gene rearrangements. Presented at the 2016 AACR Annual Meeting; April 16-20, New Orleans, Louisiana. Abstract CT007.
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Breaking News for GIST Patients – Avapritinib Approved! January 9, 2020
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Meetup at 7:00am ar Racetrac and KSU at 7:15am. We will ride to Lakewood together and have Breakfast then take off on ride. We are only doing about 100 miles because of the heat that is coming. The route is 99miles and 2hrs 10mins to 205 in downtown Rockwall.