text:
Abesim Fridge Killings: Siblings Opened Sarpong's Coffin And Gave Him Weapons To Retaliate
By Blind_Justice (self media writer) | 1 year ago
The siblings of the 15-year-old Stephen Sarpong who was murdered by Richard Appiah in Abesim, have invoked a ghostly attack on the later for the crime he has committed. At the burial site just when the pastor, family members and sympathizers had left, the siblings and other elderly friends of Sarpong opened the coffin and incanted some words onto a machete and other weapons, praying that the ghost haunts down his killer.
They later sealed the coffin after planting these weapons beside the corpse of Stephen Sarpong. They cursed that the spirit of the murdered teenager retaliates before it finally goes to rest.
As it is with the typical Akan group who believe the souls of the departed have the power to seek justice on those who are responsible for their unnatural death, Sarpong's siblings wish their younger brother will act on their wishes. To them it's all about justice, as the news surrounding investigations conducted by the Criminal Investigation Department of the Ghana Police Service that the prime suspect, Richard Appiah, claims to be mentally ill.
They think there is a grand scheme to cover up for the crime, and they are not going to let Richard Appiah go freely. It was reported that neither of the families of Richard Appiah made it to the funeral of the Stephen Sarpong, let alone send their condolences to the bereaved family.
Man incanting words on the weapons before placing it in the coffin
Through the lenses of M'akosem Television, one could tell that Sarpong was loved, and many have still not recovered from the shock about his gruesome murder by Richard Appiah. The family might have expressed confidence in the state regarding the prosecution of the prime suspect, they also believe a layer of spiritual judgement could be an excellent option.
Such a ritual performed by the siblings have become very common, and the aftermath very dire for whom that is deemed guilty. On the back of a defense that Richard Appiah acted out of abnormality, there is total confidence expressed by the siblings that Sarpong's ghost and his ancestors shall be a best judge of character and freedom for Richard Appiah.
Given the experience with such rituals, it is expected that some reaction would be observed soon on Richard Appiah. This is expected within two weeks, and surely, Ghanaians would be confronted with the news about the consequences of this ritual.
Content created and supplied by: Blind_Justice (via Opera News )
Abesim Akan Criminal Investigation Department Sarpong Stephen Sarpong
Sad: Momo Vendor Allegedly Commits Suicide Leaves Emotional Message To His Family & Friends


text:
Yeovil Town Football Club is a professional association football club based in the town of Yeovil in Somerset, England. The team currently competes in the , the fifth tier of the English football league system. The club's home ground is Huish Park, built in 1990 on the site of an old army camp. That stadium is named after their former home, Huish, known for its pitch, which had an  sideline to sideline slope. The club's nickname "the Glovers" is a reference to the history of glove-making in the town of Yeovil, which became a centre of the industry during the 18th and 19th centuries.

Founded in 1895, the club initially joined the Somerset Senior League and competed in a multitude of leagues up until the outbreak of World War II. During this time they won titles in the Southern League, Western League, Bristol Charity League, Dorset District League and Somerset Senior League. They played in the Southern League after the war ended, winning the championship in 1954–55, 1963–64 and 1970–71, before becoming members of the Alliance Premier League from 1979 to 1985. They spent the next three years in the Isthmian League, and were promoted into the Conference after finishing as champions in 1987–88. Relegated in 1995, they were promoted again two years later after winning another Isthmian League title. Yeovil won the 2002 FA Trophy Final and secured a place in the Football League after winning the Conference in 2002–03 under the stewardship of Gary Johnson. They then won the League Two title in 2004–05, before reaching the Championship with victory in the 2013 Football League One play-off final in Johnson's second spell as manager. However they suffered consecutive relegations, and were relegated once more following the 2018–19 season, ending their 16-season spell in the Football League.

Yeovil are one of the most successful non-league teams in the FA Cup, having defeated major Football League teams, most famously Sunderland in the fourth round in 1949, before going on to play in front of more than 81,000 spectators away at Manchester United in the next round. For some years, as the only Football League side in Somerset, they have had few local rivalries since Weymouth and Bath City declined simultaneously as Yeovil climbed the divisions in the 1990s and 2000s.

History

Non-League football
Yeovil Football Club was founded in 1890, and shared its ground with the local rugby club for many years. Five years later, the current club was founded and named Yeovil Casuals and started playing home games at the Pen Mill Athletic Ground. In 1907 the name Yeovil Town was adopted, which on amalgamation with Petters United became Yeovil and Petters United.  The name reverted to Yeovil Town before the 1946–47 season.

The club came to national attention as 'giant-killers' during the 1948–49 FA Cup, in which they defeated Sunderland 2–1 in the fourth round, in front of a record home attendance of 17,000. They were defeated 8–0 in the following round by Manchester United.

Between 1955 and 1973 they were champions of the Southern Football League three times, and runners-up twice. During this period, Yeovil Town applied for election to the Football League on a number of occasions, coming within a few votes of being elected in 1976. In 1979 the Glovers were founder members of the new national non-league division, the Football Conference. In 1985, they were relegated to the Isthmian League. Yeovil won that championship in 1988 and returned to the Conference.

There was success in the Bob Lord Challenge Trophy in 1990 and three years later Yeovil finished fourth in the Conference, their best finish ever. In January 1995, former Weymouth and Spurs player Graham Roberts was appointed manager, but demotion back to the Isthmian League soon followed. Yeovil secured promotion back into the Conference in 1997 after winning the Isthmian League with a record number of points – 101.

Colin Lippiatt became manager for the 1998–99 season and brought Terry Skiverton to the club as a player. Gary Johnson took over as manager in June 2001 and Yeovil won the FA Trophy in his first season in charge with a 2–0 victory over Stevenage Borough in the final at Villa Park – the club's first major trophy. Yeovil Town earned promotion to the Football League in the following season, by winning the Football Conference by a record 17 points margin, accumulating 95 points and scoring 100 goals, remaining unbeaten at Huish Park. Their team included many top players, some of whom went on to play Premier League football. Notable players include Gavin Williams who moved to West Ham United, Lee Johnson, Chris Weale, Darren Way and Adam Lockwood.

Reaching the Football League
Yeovil's first game in the Football League was a 3–1 away win over Rochdale. The Glovers finished their first season in eighth position, and reached the third round of the FA Cup before losing 2–0 at home to Liverpool. Before the game the club released a record sold only in shops in the town: "Yeovil True" reached No. 36 in the UK Singles Chart. The following season Yeovil finished as champions of League Two with 83 points, earning promotion to League One. Partway through the season the club was sold by Jon Goddard-Watts to David Webb, who took over the role of chief executive from chairman John Fry.

At the beginning of the 2005–06 season manager Gary Johnson left Yeovil for Bristol City. He was replaced by his assistant Steve Thompson and Kevin Hodges was appointed as his number two. At the season's end Thompson was demoted to first-team coach and he was replaced by Russell Slade. Around this time John Fry had bought all Dave Webb's share of the club, becoming Yeovil Town's new owner. They again reached the fourth round of the FA Cup and were drawn away against Charlton Athletic, then in the Premier League, to whom they lost 3–2.

Yeovil finished the 2006–07 season in fifth position, qualifying for the League One play-offs. In the semi-final Yeovil beat Nottingham Forest in the two-legged match 5–4 on aggregate, after losing the first home leg 2–0. Yeovil met Blackpool at Wembley Stadium in the final, but were beaten 2–0.

The 2007–08 was less successful, as Yeovil finished 18th in League One with 52 points. Russell Slade continued as Yeovil manager into the 2008–09 season, but he left the position in February 2009. After one game with assistant manager Steve Thompson acting as caretaker manager, club captain Terry Skiverton was announced as manager until the end of the 2009–10 season, with Nathan Jones as his assistant. The duo kept Yeovil in League One, with safety secured following a 1–1 draw at Tranmere Rovers.

Skiverton and Jones helped Yeovil avoid relegation in the following two seasons, but a poor start the 2011–12 campaign prompted a change of manager. On 9 January 2012, the club announced the re-appointment of Gary Johnson, with Terry Skiverton becoming assistant. The Glovers went on to again achieve safety, finishing eleven points clear of the relegation zone.

Yeovil made their best ever start in the 2012–13 season, picking up 10 points from their first four games. Yeovil finished the 2012–13 season in 4th place, reaching the League One play-offs. They reached the final on 6 May 2013 after a 2–0 home victory against Sheffield United, overturning a 1–0 loss at Bramall Lane in the first leg. On 19 May 2013, Yeovil defeated Brentford 2–1 in the League One play-off final at Wembley, reaching the second tier for the first time in their history. Striker Paddy Madden, who netted the opening goal against Brentford at Wembley, finished as the league's top scorer.

Yeovil spent one season in the Championship and, despite enjoying memorable victories over Nottingham Forest, Sheffield Wednesday and Watford, suffered immediate relegation back to League One. The club's struggles continued the following season, although the club did earn a lucrative FA Cup tie against Manchester United, which they lost 2–0 despite a "gallant challenge". Manager Gary Johnson was eventually replaced by Paul Sturrock as Yeovil suffered another relegation, returning to League Two for the first time in 10 years.

Following a poor start to the 2015/16 season, Sturrock was sacked and replaced by Darren Way, initially in a caretaker role before being named permanent manager. Way was able to lead Yeovil to safety as they finished they campaign 19th in the table.

During Way's tenure as manager, Yeovil equalled their record for heaviest Football League defeat with an 8–2 loss to Luton Town on the opening day of the 2017–18 season, although they also recorded their highest Football League victory under his leadership with a 6–0 win over Newport County in September 2018. The club also enjoyed another FA Cup tie with Manchester United in January 2018, however they lost 4–0 to Jose Mourinho's side.

Return to Non-League football
Yeovil's 16-year stay in the EFL came to an end when they were relegated during the 2018–19 season, following a 2–2 draw with Northampton Town.

Darren Sarll was unveiled as the club's new manager in June 2019 and a takeover of the club by a consortium led by Scott Priestnell and Errol Pope was announced in September 2019. On 22 April, the 2019–20 National League season was ended with immediate effect due to the coronavirus outbreak, with the Glovers fourth in the table.

In May 2022, South Somerset District Council completed the purchase of Huish Park and its surrounding land for £2.8 million from Yeovil Town Football Club's owner Scott Priestnall, with the football club becoming tenants of the Council through a leaseback arrangement.

In February 2023, it was announced that SU Glovers Limited, a company publicly fronted by Matt Uggla and former England rugby union international Paul Sackey, was in the process of completing a takeover of the majority shareholding of Yeovil Town Football and Athletic Club Limited. It was confirmed in a press conference held by the club that former majority shareholder and chairman, Scott Priestnall, would no longer be involved with the football club.

Recent seasons

Rivals

The Glovers have their strongest traditional rivalries with Weymouth, which has been described as intense, and fellow Somerset club, Bath City, with Yeovil and Bath having played one another precisely 272 times. The first game for which the grandstand at Twerton Park officially opened was an FA Cup-tie on November 12, 1932. Yeovil, at the time, were said to be much "much reviled" in Bath over the years. 5,345 watched the club beat Bath 4-2. The paper reported that the crowd were 'strangely silent',  with Bath fans stating "Losing to Yeovil always hurt". However, both rivalries have dwindled significantly  over the past decades due to the lack of competitive meetings with Weymouth and Bath. The 2020–21 National League season marked the first league encounters between Yeovil and Weymouth since the 1988–89 Football Conference season.

A rivalry with Bath City dates back to numerous Southern League and Conference meetings, being described as 'fierce' during the late 1980s and early 1990s.  However, Since the turn of the century, Yeovil and Bath moved in opposite directions across the English Football Pyramid, with the two clubs being as many as five divisions apart from one another during the 2013–14 season. Having been promoted to the  EFL Championship in 2013, Yeovil played second tier football for the first time in their history during the 2013–14 season, at the same time, Bath were struggling in the sixth tier. Whilst the club were having one of the best periods in their history, Bath City were having one their worst, thus, local animosity between Bath and Yeovil dissipated.

Similarly, Hereford United were also seen as rivals, before their dissolution in 2014, due to both clubs being fairly well matched during their time in non-league ranks.

During their time in the EFL, Yeovil fans considered both Bristol Rovers and Bristol City to be rivals. In August 2009, Yeovil played Exeter City for the first time in the league, and both clubs have shared a rivalry since, with the match often being billed as a Westcountry Derby. Swindon Town and AFC Bournemouth were also considered somewhat rivals due to geographical proximity.

Players

First-team squad

Out on loan

International representatives

Club management

Corporate hierarchy

Coaching staff

Managerial history

List of chairmen
The following men have been chairman of the club's Board of Directors:

Honours and achievements

League
Football League One (Tier 3)
Play-off winners: 2012–13
Football League Two (Tier 4)
Champions (1): 2004–05
Football Conference (Tier 5)
Champions (1): 2002–03
Isthmian League (Tier 6)
Champions (2): 1987–88, 1996–97
Southern League
Champions (3): 1954–55, 1963–64, 1970–71
Southern League (Western Section)
Champions (3): 1923–24, 1931–32, 1934–35
Western League
Champions (4): 1921–22, 1924–25, 1929–30, 1934–35
Bristol Charity League
Champions (1): 1921–22
Dorset District League
Champions (1): 1908–09
Somerset Senior League
Champions (4): 1896–97, 1901–02, 1912–13, 1920–21

Cups

FA Trophy
Winners (1): 2002
Bob Lord Challenge Trophy
Winners (1): 1989–90
Runners-up: 1991–92, 1993–94
Isthmian League Cup
Winners (1): 1987–88
Isthmian Championship Shield
Winners (1): 1988–89
Southern League Championship Cup
Winners (2): 1971–72, 1976–77
Southern Football League Cup
Winners (3): 1954–55, 1960–61, 1965–66
Runners-up: 1937–38, 1946–47, 1947–48, 1955–56, 1975–76, 1976–77, 1978–79
Western Football League Cup:
Winners (1): 1958–59
Somerset Professional Cup/Somerset Premier Cup:
Winners (25): 1929–30, 1930–31, 1932–33, 1934–35, 1937–38, 1938–39, 1946–47 (jointly with Bath City), 1947–48, 1949–50, 1950–51, 1953–54, 1954–55, 1955–56, 1956–57 (jointly with Bristol City), 1961–62, 1962–63, 1964–65, 1968–69 (jointly with Frome Town), 1972–73, 1975–76, 1978–79, 1996–97, 1997–98, 2004–05, 2021–22 (record)
Runners-up: 1928–29, 1931–32, 1936–37, 1945–46, 1948–49, 1960–61, 1963–64, 1966–67, 1967–68, 1970–71, 1976–77, 1980–81, 1984–85, 1985–86, 1991–92, 2002–03, 2010–11, 2014–15
Forse Somerset Charity Cup:
Winners: 1910–11, 1912–13

Club records

 Most overall appearances: Len Harris, 691 (1958–72)
 Most goals: Johnny Hayward, 548 (1906–28)
 Most League goals: Dave Taylor, 284 (1960–9)
 Record attendance Football League at Huish Park: 9,527 v Leeds United, 25 April 2008 (League One)
 Record attendance all time: 17,123 v Sunderland, 29 January 1949 (FA Cup Fourth Round)
 Longest serving player: Len Harris, 14 years (1958–72)
 Longest serving manager: Billy Kingdon, 8 years (1938–46)
 Highest League finish: 24th Championship, 2013/2014 season
 Highest transfer fee received: £1,200,000, Arron Davies and Chris Cohen, Nottingham Forest, July 2007
 Highest transfer fee paid: Undisclosed  (five figure sum), Pablo Bastianini, Quilmes Atlético Club, August 2005
 Highest victory in the Football League: 6–0 v Newport County, 15 September 2018
 Heaviest defeat in the Football League: 0–6 v Stevenage, 14 April 2012, 2–8 v Luton Town, 5 August 2017

Notes

References

External links

 Yeovil Town news from Sky Sports

1895 establishments in England
Association football clubs established in 1895
Football clubs in Somerset
Sport in Yeovil
Somerset County League
Dorset Football League
Western Football League
Southern Football League clubs
National League (English football) clubs
Isthmian League
Former English Football League clubs

redpajama_set_name: RedPajamaWikipedia


If you have questions about SUD or MH policies and procedures, i.e., clinical documentation, grievances, Privacy, Security, etc. please contact the ACBHCS Quality Assurance. See: QA Tech Assistance Contracts for more information on (TA) Technical Assistance.
BHCS Information Systems (IS) Department has completed the "behind the scenes" work to enable a cross-walk for the DSM-IV diagnosis codes to the ICD-10 diagnosis codes, effective October 1, 2105.


TYLER, TX (KLTV) - Chef David Wallace is kicking off your Thanksgiving sides recipes with this delicious salad.
" I could give you a dozen recipes for side dishes and it still wouldn't be enough. It's that time of year, the holiday's season. There will be meals shared with your coworkers, church members, organizations, and families. Thanksgiving, Christmas, and New Years are coming quickly," Chef David says.
Mix well and serve. Don't let the holidays put you in a HOLI-DAZE!


Teacher - What's Cooking Doc?
I teach about the power of lifestyle - how we eat, move, rest and love and how it affects our health, especially its relationship with lifestyle related diseases such as diabetes, cardiovascular diseases, hypertension, cholesterol disorders and others.
In order to help people better understand and unlock the power of lifestyle in preventing and managing diabetes, cardiovascular diseases and other lifestyle related diseases, I developed Engines of Health, a medical science evidence-based framework to help people jump start their journey to unlock the power of lifestyle to retain and regain health.
I also teach at churches, organisations and companies for their staff and members.


The WCDH Diabetes Management Department provides one on one and group class support to individuals who have diabetes and wish to improve their overall health. Our staff will educate and support you on the skills needed to achieve optimal nutrition and a healthy weight that enables one to achieve and maintain good control of their diabetes. The Diabetes Management Office is located in the WCDH Cardiac Rehab Department. Advanced registration or referral is needed for group classes or 1:1 education sessions by calling (970) 332-2387.
Karla Saffer, RN, BSN has coordinated the Diabetes Education program since 2010. Working in conjunction with all care providers at the Wray Clinic, she educates patients who are newly diagnosed with diabetes as well as supports patients in their ongoing effort to maintain control of their diabetes. Support management allows Karla to be the contact for patients who have questions or concerns that may not necessarily require a doctor's visit, but attention to the issue is needed. She teaches group classes as well as collaborates with the WRAC, the CSU Extension Office and Encore Life in health and wellness promotion.
Lori Smith, RD is the hospital dietician and provides nutritional counseling for those with diabetes and other nutritional education needs. She is active in providing healthy cooking classes and consultation throughout the Wray community.
Karla and Lori have worked together to achieve and maintain accreditation of the program through the American Association of Diabetes Educators since April, 2011.
"I was told I might have diabetes--Now what?"
Being told you have diabetes or that there is a problem with your blood glucose level can cause quite a bit of stress---and rightly so!!
Diabetes can be scary. Headlines talk of what can go wrong or maybe you've witnessed firsthand the negative effects of uncontrolled diabetes. Maybe you've been in denial that anything is wrong. That's okay. Denial protects and buffers you from difficult or shocking information. Do you feel guilty? Like you caused diabetes? If so, your first assignment is to stop the blame game and get on your own side. It's never too late to jump start your diabetes self-management program. The key is to be gentle and honest because you are your best resource for managing diabetes, (along with your diabetes educator and care provider).
Your thoughts and feelings have an enormous impact on the body. Positive thoughts have positive physiologic repercussions in the body. Let's check in with your current emotional state. Why? Making and maintaining a change often depends on your emotional state.
Which one of these statements rings true for you?
Your feelings about diabetes will change day by day. All emotions are fair game. What you do with them is up to you. What are some ways you are willing to deal with the stress of your diabetes diagnosis today?
SOURCE: Garnero, T. (2014). Your first year with diabetes: What to do, month by month. American Diabetes Association. Alexandria, Virginia pp.8-9.


text:
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class Admin_Form_CustomersGroupsForm extends Zend_Form
{

    public function init()
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        $this->addElementPrefixPath('Shineisp_Decorator', 'Shineisp/Decorator/', 'decorator');
        $translate = Shineisp_Registry::get('Zend_Translate');

        $this->addElement('text', 'name', array(
            'filters'     => array('StringTrim'),
            'required'    => true,
            'decorators'  => array('Bootstrap'),
            'label'       => $translate->_('Name'),
            'class'       => 'form-control'
        ));

        $this->addElement('hidden', 'group_id');

    }

}

redpajama_set_name: RedPajamaGithub


text:
A reversible RNA on-switch using a Vivo-Morpholino to controls gene expression of AAV-delivered therapeutics in vivo
Zhong G, Wang H, He W, Li Y, Mou H, Tickner ZJ, Tran MH, Ou T, Yin Y, Diao H, Farzan M. A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivo. Nat Biotechnol. 2019;[Epub ahead print] doi:10.1038/s41587-019-0357-y
Vivo-Morpholino used to control activity of an engineered ribozyme
https://www.eurekalert.org/pub_releases/2019-12/sri-nta122419.php
Giuseppe Ronzitti. Let's switch on AAV! Sci Transl Med. 2020;12(579) doi:10.1126/scitranslmed.aba9016 (editorial)
https://stm.sciencemag.org/content/12/529/eaba9016
Read more about A reversible RNA on-switch using a Vivo-Morpholino to controls gene expression of AAV-delivered therapeutics in vivo
FDA approves golodirsen (a Morpholino drug)
Sarepta Therapeutics Announces FDA Approval of VYONDYS 53™ (golodirsen) Injection for the Treatment of Duchenne Muscular Dystrophy (DMD) in Patients Amenable to Skipping Exon 53
https://investorrelations.sarepta.com/news-releases/news-release-details...
https://www.fda.gov/news-events/press-announcements/fda-grants-accelerat...
Read more about FDA approves golodirsen (a Morpholino drug)
Paper comparing splice-modifying efficacy of Morpholinos and 2'-O-Me phosphorothioates
McIntosh CS, Aung-Htut MT, Fletcher S, Wilton SD. Removal of the Polyglutamine Repeat of Ataxin-3 by Redirecting pre-mRNA Processing. Int J Mol Sci. 2019;20(21):5434. doi:10.3390/ijms20215434
https://www.mdpi.com/1422-0067/20/21/5434/htm
Excerpt from abstract:
"Here, we describe improved efficiency in the removal of the toxic polyglutamine tract of ataxin-3 in vitro using phosphorodiamidate morpholino oligomers, when compared to antisense oligonucleotides composed of 2′-O-methyl modified bases on a phosphorothioate backbone. Significant downregulation of both the expanded and non-expanded protein was induced by the morpholino antisense oligomer, with a greater proportion of ataxin-3 protein missing the polyglutamine tract. With growing concerns over toxicity associated with long-term administration of phosphorothioate oligonucleotides, the use of a phosphorodiamidate morpholino oligomer may be preferable for clinical application. These results suggest that morpholino oligomers may provide greater therapeutic benefit for the treatment of spinocerebellar ataxia type 3, without toxic effects."
Excerpt from introduction:
"Here, we describe efficient removal of the CAG containing exon 10 to produce a truncated ataxin-3
protein, lacking the polyglutamine tract, an isoform reported by Toonen et al. (2017) to be functionally
active [8]. Our study shows that by using the PMO chemistry, not only is exon 10 skipping enhanced
at the RNA level, but also significant downregulation of the protein with higher number of glutamine
repeats and an increase in production of the truncated protein is observed, when compared to the use
of the 20-Me PS AO chemistry. With robust splice switching efficiency and an established long-term
safety profile, the PMO oligomers described here are presented as lead pre-clinical candidates to treat
SCA3 patients."
Read more about Paper comparing splice-modifying efficacy of Morpholinos and 2'-O-Me phosphorothioates
Protein interrelationship shown by synergy of Morpholinos to different mRNAs
Oprişoreanu A-M, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG. Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. Cell Rep. 2019;29:1082-98. doi:10.1016/j.celrep.2019.09.033
https://www.cell.com/cell-reports/fulltext/S2211-1247(19)31221-5
"Using established (Hilario et al., 2010; Zhong et al., 2012) morpholinos, we found that injection of low concentrations of either morpholino alone elicited no or only weak changes of motor axon growth (Figures 6A and 6B). However, combining these morpholinos at the same concentrations led to synergistic effects on stalling of axons at the horizontal myoseptum. Of note, total morpholino load was kept at the same level for all experiments by adding non-specific control morpholino where appropriate. This indicates a genetic interaction between chodl and col19a1(Figures 6C and 6D)."
Read more about Protein interrelationship shown by synergy of Morpholinos to different mRNAs
Morpholino assay in plasma: HPLC-MS/MS Coupled with Solid Phase Micro-Extraction
Two Quantitative Assays for the Phosphorodiamidate #Morpholino Oligomer (PMO) SRP-4045 in Mouse Plasma Using HPLC-MS/MS Coupled with Solid Phase Micro-Extraction. Poster at ASMS 2017.
https://www.covance.com/latest-thinking/explore-library/detail.html/cova...
Read more about Morpholino assay in plasma: HPLC-MS/MS Coupled with Solid Phase Micro-Extraction
A broader view: Approved Gene Therapy Products 2019
Development and Clinical Translation of Approved Gene Therapy Products for Genetic Disorders.
Shahryari1 A, Jazi MS, Mohammadi S, Nikoo HR, Nazari Z, Hosseini ES, Burtscher I, Mowla SJ, Lickert H.
Front Genet. 25 September 2019. doi:10.3389/fgene.2019.00868
https://www.frontiersin.org/articles/10.3389/fgene.2019.00868/full
Read more about A broader view: Approved Gene Therapy Products 2019
Paper: Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase.
https://www.mdpi.com/1420-3049/24/18/3287
This paper describes using a microbial transglutamase to put an azide group in a peptide onto the terminal carboxylic acid of an antibody protein, then using Cu-free click chemistry to affix an antisense oligo at that site. They didn't use Morpholinos, but in principle one could.
Huggins IJ, Medina CA, Springer AD, van den Berg A, Jadhav S, Cui X, Dowdy SF. Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase. Molecules. 2019 Sep 10;24(18). pii: E3287. doi: 10.3390/molecules24183287.
Read more about Paper: Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase.
Paper on delivery in cultures: In Vitro Validation of Phosphorodiamidate Morpholino Oligomers.
Aung-Htut MT, McIntosh CS, West KA, Fletcher S, Wilton SD. In Vitro Validation of Phosphorodiamidate Morpholino Oligomers. Molecules. 2019;24(6):2922 doi:0.3390/molecules24162922
cell culture: human dermal fibroblasts, human myoblasts
electroporation (nucelofection), Lipofectin, Lipofectamine, Endo-Porter
Read more about Paper on delivery in cultures: In Vitro Validation of Phosphorodiamidate Morpholino Oligomers.
Review on compensation: mutant & morphant
Gene redundancy and gene compensation: An updated view.
Peng J.
J Genet Genomics. 2019 Jul 19. pii: S1673-8527(19)30115-8. doi: 10.1016/j.jgg.2019.07.001. [Epub ahead of print] Review.
Read more about Review on compensation: mutant & morphant
Delay in development and behavioural abnormalities in the absence of p53 in zebrafish
Delay in development and behavioural abnormalities in the absence of p53 in zebrafish.
Elabd S, Jabeen NA, Gerber V, Peravali R, Bourdon JC, Kancherla S, Vallone D, Blattner C.
PLoS One. 2019 Jul 19;14(7):e0220069. doi: 10.1371/journal.pone.0220069. eCollection 2019.
This paper reports changes in developmental timing and behavior of p53-/- mutant embryos. While previous reports had suggested that p53 is dispensable for zebrafish development, the story might not be so simple. Previously a p53 knockdown was expected to produce an essentially wild-type embryo, but on the basis of this new work the results of Morpholino co-injection with p53 Morpholinos should be interpreted cautiously for contributions to the observed phenotype due to lack of p53. Still, given the gross abnormalities of an embryo in which the p53 apoptotic response is triggered by a knockdown, eliminating these abnormalities by a concurrent p53 knockdown is likely to remain a valuable technique for revealing gene function.
Read more about Delay in development and behavioural abnormalities in the absence of p53 in zebrafish

redpajama_set_name: RedPajamaCommonCrawl


Daisy is howling that Barkbox has arrived.
The paper matches the theme. We are going camping! Daisy kept sticking her long snout on the table. She could smell yummy treats!
Hurry up and take pictures!
She had a hard time waiting when it came to picture time!
The June Theme is The Grrrreat Outdoors! NexGard has gifted us a bonus item, Zip 'N Skip Bowls. I laughed at the bear camping tip. Bella, our Rat Terrier, has chased a bear away from the farmhouse.
The June 2016 Barkbox is full of fun camping toys and treats for the doggies.
It's a Daisy baby! The Bungee Buddy Bear ($16.00) is a cuddly baby or a fun tug of war toy. It has a squeaker in the head, a crinkly neck, and a crinkly bottle full of stuffing for its body. It's a fun toy for medium and large dogs.
The Panda's Pocket Knife ($14.00) is designed by Bark and Co. It is a heavy chewer toy that both Odie and Bella loved. Imagine a giant squeaker wrapped in nylon, and you have the Panda's Pocket Knife. I am always amazed how Barkbox finds toys that fit their theme perfectly.
Daisy loved the pig ear ($3.00) once she figured out how to eat it. She cracks me up at times.
Daisy was excited to eat the Pupstream Snacks Salmon & Apple Dog Treats (5.5 oz, $10.00?). The soft treats are on the smaller size. Daisy did not care because the first ingredient is salmon and there is no chicken in it. I love giving her treats that have ingredients that I recognize. It is made in the USA.
Yummy! Daisy loves duck which is the second ingredient of the Ducky Charms Trail Mix Treats (4 oz, $8.00). It has all kinds of yummy ingredients such as kale, apple, blueberries, and more. The biscuit size is around the appropriate size for a small dog. It is a harder biscuit than the Pupstream treats and there are pieces of baked banana chips and chewy apples in the bag. It is made in the USA.
The Zip N' Skip Bowls ($25.00) are a gift from NexGard, which is a soft, chewy flea and tick medicine. BarkBox subscribers are given the opportunity to try a free trial of NexGard. I placed the bowls in my car.
Daisy eyed up the pig's ear with suspicion.
She decided it was good stuff.
She wanted to take her baby to her lair which is under our bed. All cute babies live there out of reach of Bella and Odie.
Bella decided the baby made a great chew toy.
Odie had hard time decided which toy to play with first. He's a happy-go-lucky dog.
Bella enjoyed chewing on the Panda Knife. It felt good on her gums. A note – this is a WAY bigger toy than the one included in the box for small dogs!
The Grrrrreat Outdoors theme is adorable. The items matched the theme perfectly. The dogs loved their treats and snacks. I do wish that the dog biscuit sizes were a little larger because this is the large-size dog box. It made no difference to the dogs! The Bungee Buddy Bar is safely living under the bed and being lovingly cared for by Daisy. Barkbox is the perfect box for doggy owners looking for cute toys and snacks that are themed.
What do you think of the June Barkbox? Share below!
Save $10 on your Barkbox subscription with promo code MS10X1213 though it should pop up automatically. Or get a free month with your subscription with code FREEMONTHWITHBARKBOX!
See Barkbox in the Hello Subscription Box Directory!
The Categories:Dog Subscription Boxes. Subscription Boxes for Large Dogs,Subscription Boxes for Medium Dogs,Subscription Boxes for Puppies,Subscription Boxes for Small Dogs,Subscription Boxes for Tiny and Teacup Dogs.
The Reviews: See all our Barkbox Reviews.
I have been wondering about the large dog box! I love the multi-interest Bungee Buddy Bear. I added one to my cart for now. My retriever likes to tear toys up, but she is getting a little better about it. I wish Barkbox did a multi-dog box, or mixed size. I have a 17 pounds terrier and a 70 pound retriever. And both would expect something from the box!! Would have to order two subscriptions.


text:
Q: Two subqueries in a with statement and a select statement for the outcome with pro_cra as (
  select p.productid
  from production.product p
  where p."name" like 'Crankset'
),
add_lon as (
  select a.addressid
  from person.address a
  where city like 'London'
)
select sum(s1.orderqty)
from sales.salesorderdetail s
join sales.salesorderheader s1 on s.salesorderid = s1.salesorderid
join add_lon adl on s.billtoaddress = adl.addressid
join pro_cra prc on s.productid = prc.productid

;
)

Hi guys !
I know my problem is in the last join statements. I don't know what the solution is. Can anybody help?
Update! I deleted those select statements I had. I used to check the tables they werent relevant for the code.
I get this message.
SQL Error [42P01]: ERROR: relation "add_lon" does not exist
Position: 133

A: Try this:
with pro_cra as (
select p.productid from production.product p
where p."name" like 'Crankset'),
add_lon as (
select a.addressid from person.address a
where city like 'London')
select sum(s1.orderqty) from sales.salesorderdetail s
    join sales.salesorderheader s1 on s.salesorderid = s1.salesorderid
    join add_lon adl on s.billtoaddress = adl.addressid
    join pro_cra prc on s.productid = prc.productid;

removed the select * statements with semicolons and also moved the final semi-colon to its proper place.
Does this work?

redpajama_set_name: RedPajamaStackExchange


text:
Morris struggling to adapt to post-football life
Christian Morris won't play in the Sugar Bowl, but he went to New Orleans to help him prepare for his desired job.
Morris struggling to adapt to post-football life Christian Morris won't play in the Sugar Bowl, but he went to New Orleans to help him prepare for his desired job. Check out this story on clarionledger.com: http://on.thec-l.com/1OtPDQV
Daniel Paulling, The Clarion-Ledger Published 5:13 p.m. CT Dec. 27, 2015 | Updated 8:55 a.m. CT Dec. 28, 2015
Morris: Difficult to transition to post-football life
Former Ole Miss left tackle Christian Morris (71) is making the difficult transition to life after football after suffering a neck injury in October.(Photo: BILL BARKSDALE, FOR THE CLARION-LEDGER)Buy Photo
NEW ORLEANS - Christian Morris won't play in the Sugar Bowl, but the sophomore still made the trip to New Orleans to help him prepare for his desired job.
He suffered a career-ending neck injury Oct. 24 but spent Sunday afternoon's practice watching his former teammates while wearing a team-issued gray sweatshirt and black pants. The former offensive tackle hopes to become a college coach now that he can no longer play.
Making the transition, however, has been difficult.
"Right now, as they're having practice, I had a minor setback just thinking, flashbacks and stuff," Morris said. "I feel like in my mind, if I'm not doing anything with the team as far as physical, I feel like I'm not a part of it. I just want to be out there basically with them."
The No. 12 Rebels (9-3, 6-2 SEC) play No. 16 Oklahoma State (10-2, 7-2 Big 12) on Friday (7:30 p.m., ESPN) at the Superdome in New Orleans.
Morris entered the season as the team's second-string left tackle behind junior Laremy Tunsil but didn't receive a start while Tunsil served a seven-game suspension. Morris appeared in three games last year and five this season, largely on special teams.
His injury came while making a cut block on a field goal attempt against Texas A&M as the first half expired. Morris admits he was careless on the play. He said a player stepped on his neck, causing it to become hyperextended, leading to a burning sensation.
As he walked to the sideline, his body became weak and he fell unconscious to the ground. He was out for a few moments before waking up surrounded by people.
"I was like, 'What's going on? I was just walking a second ago.'" Morris said. "I didn't really have too much to say. I didn't know what was going on. I was scared."
He was carted off the field and taken to a local hospital.
A doctor told Morris a few weeks later that he wouldn't medically clear him to return to football, leaving Morris grief-stricken. His dream was to play college football and in the NFL.
But there was a chance that another hit like the one he experienced would paralyze him.
"At the time, I was like, 'I don't want to listen to that.' I wanted to go to Peyton Manning's doctor," Morris said, laughing. "But I don't want to be that selfish to myself to go out there and get paralyzed. I couldn't hurt my family and everybody else that cares for me."
Morris suffered a similar hit while growing up. He said doctors diagnosed him then with spinal stenosis, which is a narrowing of the spaces within the spine, but didn't tell him to stop playing.
Morris' career-ending injury came about the time his aunt Linda Scales was diagnosed with breast cancer. She had a mastectomy earlier this month and is still undergoing treatment, but Morris said she is doing much better.
As is Morris.
He recently began going to the Rebels' practices after needing some time to work through the difficulty of having his career end. Morris is happy to be around his former teammates again.
"They checked on me so much," Morris said. "It feels good being beside them. Ever since I got here, I've been cheering these guys on. I've been looking to be in their lives."
Contact Daniel Paulling at dpaulling@jackson.gannett.com. Follow @DanielPaulling on Twitter.
Dandy Dozen: QB Will Rogers learned from a Brandon HS great
What Joe Moorhead learned in first season at MSU


text:
Maurice Larrouy (Toulouse, 3 de dezembro de 1872 — local e data de morte desconhecidos) foi um atirador esportivo francês. Ele foi campeão olímpico na categoria de tiro rápido 25 metros masculino nos Jogos Olímpicos de Verão de 1900 em Paris, um dos primeiros medalhistas da França na história das Olimpíadas da era moderna.

Ligações externas

Atiradores olímpicos da França
Medalhistas nos Jogos Olímpicos de Verão de 1900


text:
Martes Sport – polska sieć wielkopowierzchniowych sklepów sportowych.

Firmę w 1993 roku w Bielsku–Białej założyli Jarosław Droździk z Michałem Kowalikiem. Przedsiębiorstwo zajmuje się dystrybucją odzieży, obuwia, sprzętu i akcesoriów sportowych oraz wyposażenia trekkingowego na terenie Europy Środkowej, Południowej i Wschodniej głównie przez własną sieć salonów sportowych. Firma obecna jest w Polsce, na Ukrainie, w Czechach, a od 1998 roku również na Słowacji, Węgrzech, Rumunii, Bułgarii, Chorwacji, Bośni, Serbii, Słowenii, Mołdawii, Czarnogórze, Albanii, Macedonii Północnej, Kosowie, Łotwie, Litwie i Estonii.

Salony Martes w Polsce znajdują się głównie w galeriach handlowych. Pierwszy salon sieci otwarty został w Czeladzi w 2005. W 2015 roku sieć liczyła poniżej 200 placówek a w 2019 roku Martes Sport posiadał już  329 sklepów. Pod koniec 2021 roku liczba sklepów przekroczyła 360.

Oprócz salonów, przedsiębiorstwo prowadzi outlety marek , Elbrus, Brugi, Iguana oraz sklepy marki AquaWave. Martes Sport jest również pośrednikiem handlowym francuskich sklepów sportowych Go Sport, Tesco, Auchan, Metro GroupW 2013 i 2014 roku firma otrzymała "Gazelę Biznesu" w konkursie organizowanym przez dziennik "Puls Biznesu", w 2017 została wyróżniona "Diamentem" miesięcznika "Forbes". W czerwcu 2019 roku właściciele sieci debiutowali w rankingu "100 najbogatszych Polaków" tygodnika "Wprost", zajmując ex aequo 86. miejsce z majątkiem szacownym na 452 miliony złotych.

Martes Sport jest sponsorem bielskiego klubu BKS Stal.

Przypisy

Polskie przedsiębiorstwa handlu detalicznego
Polskie przedsiębiorstwa handlu hurtowego
Sieci handlowe w Polsce
Sieci sklepów sportowych
Przedsiębiorstwa w Bielsku-Białej


Happy Anniversary Gift Box, send Congratulations to a special couple and let them know that you are thinking of them. Sparkling wine and chocolates will make their day.
or 6 weekly interest-free payments from NZ $13.00 or 6 weekly interest-free payments with an initial payment of NZ $13.00 and weekly payments of NZ $13.00 What's this?
Happy Anniversary Gift Box is a great little celebration for a special day. Make Memories.
This Anniversary Celebration Gift is packed in a white fabric lined Memories Gift Box. A perfect box for storing mementos.
A greeting card, boxed chocolates or wine can be added to any gift, simply choose from the add ons below once you have added your gift to the on line shopping cart.


25/09/2017�� Well, I mostly use paint on my brushes and stencils so I clean everytime I use my brushes. But as I said, I also do not have these particular brushes. They look like a large paintbrush and soft so I still would treat them the same way as my paintbrushes.... Designed by Tim Holtz and made by Stampers Anonymous. Stencils are fantastic versatile tools and easy to use. Simply use a sponge or stencil brush to apply your paint or ink to the area required.
Use small brushes with precise tips to touch up around the stencil. (FYI: Stencils with a lot of detail are harder to see your mistakes. Stencils with less pattern are easier to see small screw-ups). (FYI: Stencils with a lot of detail are harder to see your mistakes.... 14/03/2017�� Paper craft creations using a variety of papers, dies, embossing folders, inks and embellishments.
Claritystamp - bringing you the highest quality in clear stamps, stencils and crafting accessories, and home of the highly-successful Groovi Parchment System.... I chose Dawn McVey�s sketch and decided to use her take on it as inspiration, too. This was my first time using stencil brushes and I really LOVE �em! SO much easier than using a blending tool!!
Made from long lasting hog bristles for all art & craft activities. Contains 3 short and stubby stencil brushes.... Using a dry stencil brush, lightly load paint onto brush and dab-dab-dab any excess on an unused portion of your paper plate (see video tutorial below) In a straight up and down motion, apply paint lightly to your stencil.
How To Stencil A Faux Tile Feature Wall With Modern Masters Metallic Paint Cutting Edge Stencils is going to transform this plain black wall into a gorgeous faux tile accent wall with one tile stencil and two different colored metallic paints.
Using a dry stencil brush, lightly load paint onto brush and dab-dab-dab any excess on an unused portion of your paper plate (see video tutorial below) In a straight up and down motion, apply paint lightly to your stencil.
14/03/2017�� Paper craft creations using a variety of papers, dies, embossing folders, inks and embellishments.
Stack the stencils together and use a hole-punch to create registration marks (see Step 4) in each corner of the stencil. These act as a guide to keep your design consistent. These act as a guide to keep your design consistent.
Use small brushes with precise tips to touch up around the stencil. (FYI: Stencils with a lot of detail are harder to see your mistakes. Stencils with less pattern are easier to see small screw-ups). (FYI: Stencils with a lot of detail are harder to see your mistakes.


When you're dealing with halitosis, you can't live life to the most-est.
There's mounting evidence to indicate that the effects of gum disease may go far beyond your dental health, potentially threatening your overall health in some very serious ways. Here's what you need to know.
Time spent with loved ones on vacation is precious, and memorializing these moments with photos gives you something to smile about for years to come. To make sure your vacation photos feature smiles full of white teeth, here's how we can help.
Fear and anxiety about going to the dentist is common. But if your fear leads to panic and avoidance, you should consider sedation dentistry. Learn more about dental anxiety and how sedation dentistry can get you comfortably into the dental chair.
Veneers provide a solution to many cosmetic dental issues. If you're not comfortable with the appearance of your smile, dental veneers may be the right choice for you. Here's why.
In a perfect world, your wisdom teeth would come in correctly and give you no problems. In reality, this third set of molars can be a real headache. But whether you need to have them removed depends on various factors.
When you have damage or decay to a tooth that requires a root canal procedure, the dental CT scan is ideally suited for preparation.
If you suffer from two of the most common sleep disorders, sleep bruxism or obstructive sleep apnea, the solution might be a custom-fitted oral mouthguard or oral appliance from your dentist.
Are you aware that tooth extractions require a nominal amount of healing afterward to ensure the wound closes successfully in your mouth? When you prepare for a tooth extraction, it is essential to plan ahead so your body has the best recovery possible.
Cosmetic dentistry is a form of dentistry designed to improve the look of teeth that have been damaged, discolored, or lost. Not all forms of cosmetic dentistry are designed to protect your teeth or fix the damage, they are designed to improve their look.
A diagnosis of cancer is a life-changing event. No aspect of your life is left unaffected. While you are fighting cancer, it's important to keep up with your oral health regimen, and visit your dentist on a regular basis.
The American Dental Association recommends having a routine dental exam performed every six months. Not only will this remove plaque and tartar from your teeth it will also detect any oral health problems.
Do you feel ashamed of your smile? Do you wish your teeth looked better? If so, come in today at in , , and talk to us about dental veneers! Our team of professionals is happy to help you understand more about dental veneers.
Did you know mouthwash can give your smile a fresh feel? Well, it's true! Having a clean and fresh smile not only feels great but gives you fantastic breath and helps keep your smile in healthy shape.
Improve your oral health by chewing gum after meals. If you are seeking alternative dental tools that can be used to help keep your mouth fresh and your teeth clean, you may want to consider sugarless chewing gum.
You may already understand that there are several steps you can try if you're hoping to keep your teeth healthy and strong. Have you ever wondered about the benefits of an inlay or onlay?
Do you have a hard time taking your child to because the appointments seem stressful and difficult? If so, our dental team has some helpful tips that can help your child have a more positive and successful dental appointment.


text:
Void - in Music
Void (band), a Washington, D.C.-based hardcore punk band
Void (album), the debut full-length studio album by the American post-metal band Intronaut
VOID (Video Overview in Deceleration), by the Flaming Lips
"Void Malign", a song by the band Grendel
ØØ Void, the second album by Sunn O)))
"Into the Void" (Black Sabbath song), from the album Master of Reality
Throne Of Void, an album by Isole
We Are the Void, a 2010 studio album by Dark Tranquillity
"Love in a Void", the B-side to Siouxsie And The Banshees' "Mittageisen" single
Destroyer of the Void, a 2010 album by the band Blitzen Trapper
The Magnificent Void, a 1996 album by ambient musician Steve Roach
Touching the Void, a 1989 book about a near-fatal attempt to climb Siula Grande
Touching the Void (film), a 2003 film based on the book
Void (Sonic Shuffle), a character in the video game Sonic Shuffle
Void Indigo, a comic book series written by Steve Gerber and drawn by Val Mayerik
Voids, the villains in the Zbots action-figures franchise
VOID (NaNa album), a 1999 album by Japanese pop/rock musical duo NaNa
Read more about this topic: Void
Famous quotes containing the word music:
"I am advised to give her music a' mornings; they say it will
penetrate."
"A woman's two cents worth is worth two cents in the music business."
—Loretta Lynn (b. 1930)
Terms related to void:
Wikipedia Music (Creative Commons)


text:
title: "Databending images with a command line audio processing utlity"
categories:
  - tutorial
tags:
  - new-media-art
  - glitch
---

Interesting things happen when you edit images like music. [Glitchet](http://www.glitchet.com/resources) lists some resources for how to get started on databending. Using Audacity to databend images is somewhat cumbersome and that is when I found [sox](hhttps://sourceforge.net/projects/sox/). It is called "the Swiss Army knife of audio manipulation" on its [man page](https://linux.die.net/man/1/sox). Thanks to the sox mailing list where Mr. Rullgård helped me with the commands!

## Prerequisites
I am on a Windows 10 computer so I will be using the Command Prompt.

* Sox, I am using v14.4.2 downloaded from [Sourceforge](https://sourceforge.net/projects/sox/files/sox/)
* Added to PATH[^1], you'll need to do this to use sox as a command on Command Prompt
* [ImageMagick](https://www.imagemagick.org/script/download.php), to make gif from a bunch of images with 'convert'

## Process

In this tutorial, I will use sox to databend an image and then we'll make 100 versions of that image to make a gif. Everything inside the shell. I am starting with this image, you can start with any image in any uncompressed image format.

![alt text](/images/water.bmp)

Our idea here is to ask sox to apply an audio effect to the image. Sox commands will look like `sox [input] [output] [effects]`. We need to make sure the files are treated as raw data and also make sure the BMP header is left untouched.

```
sox -t ul -c 1 -r 48k water.bmp -t ul water_out.bmp trim 0 100s : echo 0.8 0.88 60 0.5
```

There are a couple of things happening here, let me break it down:

* `-t ul` -- We don't want sox to infer filetype from the filename so this sets the filetype to a headerless raw format 'ul'. The name 'ul' is an abbreviation of μ-law which is an algorithm used in PCM systems.
* `-c 1` -- Sets number of channels as 1. Although this is default for all raw formats, it doesn't hurt to set it explicitly.
* `-r 48k` --  Sets the sample rate to 48k
* `trim 0 100s :` -- Skip first 100 samples
* `echo 0.8 0.88 60 0.5` -- This follows the format taken from man page, `echo gain-in gain-out <delay decay>`

We get this image, *water_out.bmp*

![alt text](/images/water_out.bmp)

Let's extend this to make 10 images with a simple loop inside the command prompt, using a for-loop [^2]

```
for /l %i in (1,1,10) do sox -t ul -c 1 -r 48k water.bmp -t ul water%i.bmp trim 0 100s : echo 0.8 0.88 %i 0.5
```

We can also take this further and make 100 images!

```
for /l %i in (1,1,100) do sox -t ul -c 1 -r 48k water.bmp -t ul water%i.bmp trim 0 100s : echo 0.8 0.88 %i 0.5
```

Make a gif with all of those images,

```
convert -delay 10 -loop 0 water*.bmp animate_water.gif
```

This is the end result,

This was a basic example showing how to work with sox and images, you can of course extend this to all of the audio effects possible with sox. Check out their man page for more examples. Let me know what you make with this!

### Added on 8th May, 2018

I posted the gif in the end to /r/Glitch_Art and it reached the subreddit's homepage! [In the comments](https://www.reddit.com/r/glitch_art/comments/8hnilz/databending_water_tutorial_in_comments_gif/), /u/ddneva asks something,

> ddnava
> I'm actually more curious about what am I actually watching instead of how to do it

I wrote [an explanation as a reply in the comments](https://www.reddit.com/r/glitch_art/comments/8hnilz/databending_water_tutorial_in_comments_gif/dymmjwh) there but I think it will be helpful if added here too. Pasting my comment as-is
{: .notice}

## Why This Happens

You're watching an echo travel across the image as delay is varied. The effect used here took four parameters, it was `echo gain-in gain-out delay decay`. Think of it as taking every pixel value in the image and processing it through the diagram below,

LibSoX source visualizes it with this ASCII diagram,
```

 *        * gain-in                                ___
 * ibuff -----------+---------------------------->| + |
 *                  |       _________             | + |
 *                  |      |         |  * decay 1 | + |
 *                  +----->| delay 1 |----------->| + |
 *                         |_________|             ---
 *                                                  |
 *                                                  | * gain-out
 *                                                  |
 *                                                   +----->obuff
```
As we change the delay value inside the for loop it generates an echo of variable delays. Although this operation doesn't happen for the image as a whole. But you can as well think of it in terms of a whole image. When I generated 100 images, sox somewhat did this

    Image + [Image_Copy(shifted by 1 unit) * gain value]

    Image + [Image_Copy(shifted by 2 unit) * gain value]

    Image + [Image_Copy(shifted by 3 unit) * gain value] and so on.

Thus, when we put it all back together. It looks like this.

[^1]: You can do this from command line too, open command prompt and type `setx path "%path%;C:\Program Files (x86)\sox-14-4-2"` check the directory name to make sure it exists and the version number is correct.

[^2]: Check 'help for' inside Command Prompt for the help page. It is also available [here](https://ss64.com/nt/for_cmd.html). When iterating through a list of numbers, the syntax is 'for /l %i in (start, step, end) do <cmd>'