Viano Rose food is the ideal fertiliser for shrub- and climbing rose, rosebushes and standard (cutting- )roses. Contains a high potash content to increase flowering and flower bud formation. With extra magnesium for bright colours. Soft acting fertiliser that guarantees a longer preservation after cutting. Works up to 3 months. This organo- mineral premium fertiliser imcreases the humus content in the soil and improves soilstructure and micro- life.
Viano Rhododendron and azalea food is an organo-‐ mineral fertiliser in crumbs. Enriched with magnesium for bright colours. Due to the presence of a high potash concentration, this soft & acid working fertiliser ensures abondant flowering and an extended preservation of the flowers after cutting.. Viano Rhododendron and Azalea food can be used as a pH corrector in soils with to much calcium. Works up to 3 months. Can be applied on soils with to high calcium (Ca)concentrations were it will lower the pH.
An organo- mineral fertiliser with magnesium that prevents the prematural shute of needles and brown colouring of leaves in conifers, taxus and evergreen shrubs. With extra magnesium for bright green colours. This fertiliser contains extra potassium to guarantees strong and healthy plants! Viano fertiliser for conifers and Taxus improves humus content in the soil and works up to 2 months. This fertiliser can be appllied during the whole growing season and assures a better soil structure and soil life. Incorporate superficially to get the best results.
An organo- mineral NPK fertiliser with magnesium for deep green coloUrs. This slow and soft acting fertiliser (up to 3 months) improves soil structure due to the organic components . Guarantees strong and healthy plants with a higher resistance against drought and frost.Viano Boxwood plantfood can be used for Boxwood in full ground or in pots and container.
A organo- minEral fertiliser specially composed for fertilisation of ornamental leafplants and soilcovering plants in the garden. Soft acting fertiliser that improves humus content in the soil. With extra magnesium for bright coloUrs. Can be applied for shadowplants. Works up to 3 months and gives soil covering plants all they need for a healthy development. This fertiliser can be applied while planting or during maintenance in full ground or in cointainer.
Viano lavender food is the ideal fertiliser lavender and other aromatic (herb) plants. Contains a high potash content to increase flowering and flower bud formation. With extra magnesium for bright colours . Soft acting fertiliser that guarantees a longer preservation after cutting. Works up to 3 months. This organic premium fertiliser imcreases the humus content in the soil and improves soilstructure and micro- life.
Viano organic food for citrus, olives and figues is an natural fertilizer for the fertilisation in container or in open soil. The high potash content ensures a increased formation of flower buds, more fruits, strong and healthy fruits with a longer preservation time after harvesting.
With Viano organic food for citrus, olives and figues , fruits become more tasty. This fertiliser can be applied during plantation or as a stock fertiliser during growing season.
Works up to 2 months.
Viano Geranium and Surfinia fertiliser is the ideal feed and contains a high potash content to increase flowering and florwer bud formation. With extra magnesium for bright colours . Soft acting fertiliser that guarantees a longer preservation. Works up to 2 months. This organo- mineral premium fertiliser imcreases the humus content in the soil and improves soilstructure and micro- life.
Viano Clematis food is the ideal fertiliser for Clematis and other climbing plants. Contains a high potash content to increase flowering and florwer bud formation. With extra magnesium for bright colours . Soft acting fertiliser that guarantees a longer preservation. Works up to 2 months. This organo- mineral premium fertiliser imcreases the humus content in the soil and improves soilstructure and micro- life.
Organic- mineral fertiliser specially for the fertilisation of flowering shrubs, flowerbeds and ornamental garden plants. With extra magnesium for vivid colours. This fertiliser guarantees a long working period (up to 3 months) . Due to the specific composition,Viano fertiliser for flowerbeds and flowers ameliorates the soil structure and enhances soil life. Due to the high potash content a better flowerbud development and a abundant flowering can be expected.
Organic based fertiliser specially for the fertilisation of flowering shrubs, flowerbeds and ornamental garden plants. With extra magnesium for vivid colours. This fertiliser guarantees a long working period (up to 3 months). Due to the specific composition, Viano fertiliser for flowerbeds and flowers ameliorates the soil structure and enhances soil life. Due to the high potash content a better flowerbud development and a abundant flowering can be expected.
An organic fertiliser for general use in ornamental, kitchen, herb- fruitcrops and vegetable garden, ornamental garden and lawn. It is authorized for use in organic farming and guarantees a long and soft action period (3 months).. It offers the plant a wide range of essential nutrients, activates soil lif and improves soil structure. A real booster for any crop. Can be generally applied from spring till autumn but gives the very best results when applied in fruitforming vegetables and fruit garden. Viano Universal bio fertliser is to incorporate before planting or to use as a maintenance fertiliser during growing season.
RHS endorses organic based lawncare products from Viano!
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[Cite This]
Albert Bandura
"People who believe they have the power to exercise, some measures of control over their lives are more healthier, more effective, more successful than those who lack faith in their ability to effect changes in their lives." – Albert Bandura
It is not wrong to say that Albert bandura is a living legend in the world of psychology, He is truly a living legend who has proposed theories that proved his mettle against and claimed him fame to the levels of B.F Skinner, Sigmund Freud and Jean Piaget. His name is mentioned against some famous psychologists, including the likes of Sigmund Freud, for his works encompasses around the major contributions in the major spectrums of psychology ranging from social cognitive theory, therapy to personality psychology. Born in Mundare, Canada in 1925 and brought up in a small farming community in Canada after doing his elementary school he had no higher learning opportunities in that city to quench his thirst for knowledge so that lead him into becoming a self motivated learner. He did his BA from University of British Columbia. Then he did his MA from University of Iowa in theoretical psychology.
His first book is based upon the theories and observations behind adolescent aggression. He conducted 'Bobo doll experiment' in 1961 which gained fame among the internationally acclaimed psychologists, he conducted this experiment on a young woman spanking a doll, this a kind of doll which sways back and forth when hit by any object as in hammer ,more commonly known as bob clown. The woman objected the clown to physical abuses and shouting out "sockeroo!", Bandura filmed this video and showed it to a bunch of kids in kindergarten who liked it a lot as per their natural instincts, so they were give a set of bobo dolls and little hammers in front of a bunch of observers to watch on their behaviors. Naturally the little started hammering the bobo dolls with all enthusiasm, so by his experiments he concluded that there is a phenomenon known as observational leaning or modeling, He coined the term social learning theory as an inference for this experiment. The bobo doll experiment provided the basis for child psychology which says that a child learns by observing and repeating what he sees and perceives in the environment.
According to the social learning theory there are some necessary traits that are to be adopted while learning in a social situation, these traits are attention, retention, reproduction, motivation and self-regulation. His ground breaking book based on social learning theory published in 1977 set a new direction for modern psychology in 1980's. His acclaimed publication in 1986 Social Foundations of Thought and Action: A Social Cognitive Theory, in which he proposed that human beings are in control of themselves and have a proactive approach in dealing with life situations and with the choices they make for themselves. He also wrote a book on self-efficacy which is the art of practicing self-control which got published in 1977.
Albert Bandura is a major psychologist of this era who has contributed a great deal in the history of modern psychology. His honorable accolades includes the most youngest president of the American Psychological Association (APA) where he served as the 82nd president of the prestigious association, member of editorial board of the nine psychological journals and Grawmeyer award winner in psychology. His ground breaking theory in self regulated learning earned him the award for distinguished scientific contributions from APA. He also received 16 honorary degrees from universities of repute. As a higher recognition of his works American Psychological Association awarded him with a gold medal recognizing and appreciating his lifetime contributions towards psychology.
Buy Books by Albert Bandura
How to Cite This Page
Albert Bandura. (2014). FamousPsychologists.org. Retrieved 04:07, February 1, 2023 from https://www.famouspsychologists.org/albert-bandura/
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" Albert Bandura," FamousPsychologists.org, https://www.famouspsychologists.org/albert-bandura/ (accessed February 1, 2023).
Albert Bandura [Internet]. FamousPsychologists.org; 2014 [cited 2023 February 1]. Available from: https://www.famouspsychologists.org/albert-bandura/.
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Mouser Electronics and Mobix Labs Sign Global Distribution Deal
Mouser Now Shipping Mobix Labs' True Xero Active Optical Cables Irvine, Calif., January 19, 2022 – Mouser Electronics, Inc. announces a global distribution agreement with Mobix Labs Inc., a fabless RF component and active optical cable company focused on next-generation connectivity technologies. According to the agreement, Mouser now offers customers access to Mobix Labs' antenna solutions, […]
Mobix Labs Introduces Full Line of True Xero Active Optical, Direct Attach Cables and Transceivers
Mobix Labs introduced its new line of True Xero™ latency interconnect cables and transceivers for consumer, professional audio-video and enterprise applications. Pictured are a Mobix Labs USB DPA cable (left), transceiver (middle) and direct attach cable (right). Product Mix Serves Consumer, Pro-AV and Enterprise Market Segments, Company Executives Available at CES 2022 IRVINE, Calif., January […]
James J. Peterson Named Executive Chairman of Mobix Labs
New Title Represents Expanded Role in Company's Strategic Direction Peterson was elected board chairman of Mobix Labs earlier this year, and hepreviously served as CEO and chairman of Microsemi Corporation, now Microchip Technology Inc. "Jimmy has been instrumental in the growth and trajectory of Mobix Labs since we launched operations last year," said Fabian Battaglia, […]
Mobix Labs Opens Design Center in Australia
Company Plans to Expand Design Centers Globally IRVINE, Calif., October 19, 2021 – Mobix Labs Inc., a global connectivity solutions provider for wireless mmWave 5G and wired high bandwidth cable networks, today announced it has opened a new design center in Sydney, Australia, with the hiring of a veteran team of engineers who have strong […]
Mobix Labs Names Phil Sansone as Vice President of Worldwide Sales
IRVINE, Calif., October 5, 2021 – Mobix Labs Inc., a global wireless and wired connectivity solutions provider, today announced the appointment of Phil Sansone as vice president of worldwide sales. Sansone brings 30+ years of worldwide sales experience to Mobix Labs. In his role at the company, Sansone will be responsible for global sales of Mobix Labs' True5G™ […]
Mobix Labs Named 'Outstanding Emerging Technology Company' by Octane
IRVINE, Calif., September 27, 2021 – Mobix Labs Inc., a global wireless and wired connectivity solutions provider, today announced it has received the 2021 High Tech Award for "Outstanding Emerging Technology Company" by Octane, Southern California's leading accelerator. "A company is only as good as its people, and we are lucky to have the most talented and hardworking […]
Mobix Labs to Acquire Cosemi Technologies
Combined IP Bridges the Gap Between Wired and Wireless Connectivity to Offer True Xero™ Latency Solutions for 5G mmWave Networks and Other Applications Cosemi Founder and CEO Nguyen X. Nguyen, Ph.D., to Join Mobix Board of Directors Irvine, Calif., July 15, 2021 – Mobix Labs Inc., a fabless RF (radio frequency) component company focused on […]
Mobix Labs Approved for the Japan External Trade Organization's Invest Japan Support Program
Organization to Promote Company's 5G mmWave Technology in Japan IRVINE, Calif., Jun. 12, 2021— Mobix Labs Inc., a fabless RF (radio frequency) component company focused on next-generation wireless technologies, today announced that it has been approved for the Invest Japan Support Program offered by the Japan External Trade Organization (JETRO), a government-related organization that helps […]
Mobix Labs Signs Supplier Agreement with Wutong Group
IRVINE, Calif., June 23, 2021 (GLOBE NEWSWIRE) — Mobix Labs Inc., a fabless RF (radio frequency) component company focused on next-generation wireless technologies, today announced it has entered into an agreement with the Wutong Group, one of the leading global suppliers of radio frequency connection systems. Under the terms of the agreement, China-based Wutong Group […]
Mobix Labs Signs Distribution Agreement with Acromax to Service Greater China
Company's Chip Antennas Already Being Sampled by Customers IRVINE, Calif., Jun. 08, 2021 (GLOBE NEWSWIRE) — Mobix Labs Inc., a fabless RF (radio frequency) component company focused on next-generation wireless technologies, today announced that it has entered into an agreement with Acromax Inc., a distributor of semiconductors and solutions in Mainland China, Hong Kong and […]


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All Content Periodicals Books All Content
Home / Concepts / Chromosomes
More general concepts than this:
Genetic Elements
More specific concepts than this:
Chromosome Types,
Chromosome Structure
Chapter [37] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Chapter
Article [12] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Article
Frontmatter [7] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Frontmatter
MagazineArticle [4] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/MagazineArticle
Continuous Publication Journal [2] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/ContinuousPublicationArticle
Backmatter [1] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Backmatter
Contributors [1] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Contributors
2017 [3] http://pub2web.metastore.ingenta.com/ns/yearOfPublication 2017
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Teaching Genetic Linkage and Multiple Crossovers with Sets of Cards as Chromosomes †
Author: Richard H. Heineman
Citation: Heineman R. 2017. Teaching genetic linkage and multiple crossovers with sets of cards as chromosomes † . 18(3): doi:10.1128/jmbe.v18i3.1366
DOI 10.1128/jmbe.v18i3.1366
TMathematical models help students identify and understand underlying scientific patterns, while improving and reinforcing quantitative skills. However, they are often omitted from introductory undergraduate science classes. As a result, math-heavy theories, like optimal foraging theory, are often omitted from these classes. While optimal foraging easily fits into the curriculum of any class discussing food webs and energy transfer, this theory is often not explained until the graduate level. In this article, I describe a quantitative-rich lesson paired with a hands-on active learning module (or alternatively, a demonstration). This lesson was used in an introductory-level environmental science course but is suited for a range of biology and environmental science classes or laboratories.
Kinesthetically Exploring Chromosomes from Cell Division and Meiosis to Mendelian Genetics †
Citation: Heineman R. 2017. Kinesthetically exploring chromosomes from cell division and meiosis to mendelian genetics † . 18(3): doi:10.1128/jmbe.v18i3.1357
Physical models are widely used to help students understand what happens to chromosomes during mitosis and meiosis. They can also be used to explicate the genotype-phenotype map. This laboratory exercise combines both these subjects at a level appropriate for a college-level genetics class. Students first trace the steps of mitosis and oogenesis, with special attention to ploidy and chromosome count. They then use the alleles arrived at by this process to build marshmallow creatures. Finally, they are introduced to more complicated modes of inheritance such as sex-linked traits, incomplete dominance, multiple alleles, and polygenic traits. Evidence of positive student attitudes and learning outcomes is presented.
A Hands-On Activity to Demonstrate the Central Dogma of Molecular Biology Via a Simulated VDJ Recombination Activity †
Author: Pamela A. Marshall
Citation: Marshall P. 2017. A hands-on activity to demonstrate the central dogma of molecular biology via a simulated vdj recombination activity † . 18(2): doi:10.1128/jmbe.v18i2.1277
Essential or enduring understandings are often defined as the underlying core concepts or "big ideas" we'd like our students to remember when much of the course content has been forgotten. The central dogma of molecular biology and how cellular information is stored, used, and conveyed is one of the essential understandings students should retain after a course or unit in molecular biology or genetics. An additional enduring understanding is the relationships between DNA sequence, RNA sequence, mRNA production and processing, and the resulting polypeptide/protein product. A final big idea in molecular biology is the relationship between DNA mutation and polypeptide change. To engage students in these essential understandings in a Genetics course, I have developed a hands-on activity to simulate VDJ recombination. Students use a foldable type activity to splice out regions of a mock kappa light chain gene to generate a DNA sequence for transcription and translation. Students fold the activity several different times in multiple ways to "recombine" and generate several different DNA sequences. They then are asked to construct the corresponding mRNA and polypeptide sequence of each "recombined" DNA sequence and reflect on the products in a write-to-learn activity.
Author: Christoph Weigel
Source: , September 2016, 11:410-410; doi:10.1128/microbe.11.410.1
Publication Date : September 2016 DOI 10.1128/microbe.11.410.1
At the heart of every cell's "operating system" are the ribosomes: ingenious nanomachines that translate messenger RNA (mRNA) into proteins that cells need to build and maintain themselves. Ribosomes were first described by the electron microscopist George Palade in a 1955 paper, in which he contemplated the electron dense particles lining the endoplasmic reticulum of rat pancreas cells: "The relationship between the membrane of the endoplasmic reticu-lum and the small granules deserves special consideration." Indeed, they deserved and got consideration: today PubMed lists over 50,000 papers dealing with ribosomes—comparable to the number of ribosomes in rapidly grow-ing E. coli cells. With such numbers, it's no wonder cells spend roughly 2/3 of their energy budget on the synthesis of ribosomes and translation!
Changes in Chromosome Structure Regulate DNA Replication Initiation
Source: , October 2015, 10:412-412; doi:10.1128/microbe.10.412.1
Publication Date : October 2015 DOI 10.1128/microbe.10.412.1
Bacterial chromosomes change organization and structure during the cell cycle, for reasons as yet unclear. Now David Magnan and David Bates of Baylor College of Medicine, Houston, show in Escherichia coli that programmed changes in chromosome structure may regulate initiation of DNA replication. Negative supercoiling, which favors duplex melting, is required to initiate replication. Based on their previous observations, they posited that structural differences before and after initiation of replication changed chromosome supercoiling. They found that artificially tethering the chromosome to the cell membrane decreased negative supercoiling, and blocked replication initiation without affecting other DNA metabolic processes. "This finding may reveal new targets for antibiotics, and may explain why existing antibiotics that affect DNA supercoiling are so effective," says Bates. "More significantly, this research may lead to better understanding of bacterial cell cycle control, which is a black box, as well as eukaryotic replication initiation, which is also sensitive to changes in chromosome structure."
Author: Merry Youle
In the course of time, bacteria have opportunities to incorporate phage genes into their genomes. Frequently these genes originated in a temperate phage that integrated its DNA into the host's chromosome and then was stranded there by a mutation that inactivated its escape mechanism. Most of the prophage genes will accumulate mutations and degenerate into pseudogenes that are eventually eliminated. Currently useful genes, such as those that encode virulence factors, will be maintained. All are potentially valuable hand-me-downs, nonessential genes that the cell can tinker with and perhaps adapt to its own purposes.
The Joint Genome Institute Offers Resources Beyond a Core Facility
Authors: David Gilbert, Nikos Kyrpides, Susannah Tringe, Axel Visel, and Tanja Woyke
Source: , July 2015, 10:289-293; doi:10.1128/microbe.10.289.1
Publication Date : July 2015 DOI 10.1128/microbe.10.289.1
The U.S. Department of Energy (DOE) Joint Genome Institute (JGI) was established in 1997 to consolidate the department's programs and resources in DNA sequencing, informatics, and technology development. Soon after, the University of California, which manages the Lawrence Berkeley National Laboratory, where JGI was first situated, leased lab and office space in nearby Walnut Creek to house JGI activities. The early focus for JGI was the Human Genome Project. After its scientists completed their sequencing of three human chromosomes, however, JGI broadened its mandate in 2004 to become a national user facility, which now boasts thousands of users worldwide.
Review of: iBioSeminars and iBioMagazine
Citation: Marshall P. 2013. Review of: ibioseminars and ibiomagazine. 14(2):285-286 doi:10.1128/jmbe.v14i2.657
DOI 10.1128/jmbe.v14i2.657
Review of: iBioSeminars (http://ibioseminars.org/) and iBio-Magazine (http://ibiomagazine.org/).
Do Bacteria Have Sex?
Author: Rosemary J. Redfield
Source: Microbes and Evolution , pp 139-144
Bacteria do not reproduce sexually; they have one set of genes rather than two, and their cells simply divide-they never fuse with each other like eggs and sperm. Short pieces of the DNA that genes are made of sometimes move from one bacterial cell to another, and this can randomly mix their different versions of genes if it happens often enough, just like real sexual reproduction does. For a long time microbiologists assumed that these parasites were a bacterial way of having sex-that is, that the evolutionary forces responsible for our sexual reproduction had indeed acted on bacteria too, producing ways to move DNA between cells. Nutrients and temperature conditions are usually very different from those the bacteria experience in their natural environments, partly because they are more convenient and partly because one really does not know what the natural conditions are. But studying bacteria under such unnatural conditions makes it very easy to misinterpret what they do. Most bacteria use nutritional signals, just as the DNA-equals-food explanation predicts. Just as people who read only the newspaper headlines think every lottery ticket is a big winner, a scientist who reads only the research papers about bacterial genome sequences could easily conclude that every new gene is an improvement. In either case, the discovery that bacteria do not have sex means that a big key to the puzzle of the evolution of sex will come from finding out why bacteria need so much less mixing than plants and animals.
Sexual Difficulties
Author: Howard Ochman
Sex in bacteria is not linked to reproduction—parents passing genes to offspring (via that cycle of sex and reproduction) is the opposite of what we consider to be sex in bacteria. In bacteria, sex is the inheritance of DNA from any source except the parental cell. Asexual reproduction results in an exponential increase in cell numbers: it takes 10 generations to attain 1,000 cell replications and only 70 generations to produce 1021 cells, equal to the estimated number of stars in the universe. The generation times of bacteria are short, on the order of hours or days for those species considered to be "slow growing." Therefore, relatively little time is needed for cell numbers to reach the point where each nucleotide in a genome has been subjected to at least one mutation. Transformation is the uptake of DNA from the environment. Any advantage tendered by acquired DNA is secondary to the real reason that bacterial sex evolved. If a gene is acquired from a divergent source, both the donor and recipient can have identical copies despite the fact that the organisms themselves are unrelated. Expanding this to the point where every gene has the potential to transfer to any organism from any source, and this might cause some systematists to lose sleep: rampant gene transfer would render useless their efforts to assign microbes to meaningful groups and to reconstruct the evolutionary links between organisms, since the closest relatives may be no longer be the most closely related.
Microbial Symbiosis and Evolution
Author: Nancy A. Moran
This chapter talks about the symbiosis of microbes and mitochondria. Mutations, including deletions of DNA, happen constantly, so the unused genes have long since disappeared from the genomes of mitochondria that inhabit our cells. In fact, mutations are still raining down on the genomes of our mitochondria, and these continue throughout our lives, contributing to various diseases and disorders. These malfunctions are the main reason we even think about our mitochondria. Another reason the mitochondria can live with so few genes is that many of the ancient genes of the mitochondrial ancestor have changed addresses, moving from the chromosome of the mitochondrion to our own chromosomes but sending their working products back to the mitochondrial homeland to carry out needed work. Some symbiotic bacteria have fewer than 200 genes, and the symbionts we call organelles can have even fewer, as in our own mitochondria with their miserly 15. Buchnera uses its ancestral genes for making the amino acids tryptophan and leucine, which are nutrients that a host needs. But these genes have been copied many times on tiny extra chromosomes (plasmids), an arrangement that allows hyperproduction of the amino acid products. Both symbionts and domesticated breeds can evolve extreme features over short periods on an evolutionary time scale. These extremes are produced mostly by exaggerating some functions, rather than by acquiring anything truly novel. And just like our domesticated animals and plants, symbionts themselves can be large, typically much larger than their free-living wild relatives.
Homologous Recombination—Experimental Systems, Analysis, and Significance
Author: Andrei Kuzminov
Citation: Kuzminov A. 2011. Homologous Recombination—Experimental Systems, Analysis, and Significance, EcoSal Plus 2011; doi:10.1128/ecosalplus.7.2.6
DOI 10.1128/ecosalplus.7.2.6
Homologous recombination is the most complex of all recombination events that shape genomes and produce material for evolution. Homologous recombination events are exchanges between DNA molecules in the lengthy regions of shared identity, catalyzed by a group of dedicated enzymes. There is a variety of experimental systems in Escherichia coli and Salmonella to detect homologous recombination events of several different kinds. Genetic analysis of homologous recombination reveals three separate phases of this process: pre-synapsis (the early phase), synapsis (homologous strand exchange), and post-synapsis (the late phase). In E. coli, there are at least two independent pathway of the early phase and at least two independent pathways of the late phase. All this complexity is incongruent with the originally ascribed role of homologous recombination as accelerator of genome evolution: there is simply not enough duplication and repetition in enterobacterial genomes for homologous recombination to have a detectable evolutionary role and therefore not enough selection to maintain such a complexity. At the same time, the mechanisms of homologous recombination are uniquely suited for repair of complex DNA lesions called chromosomal lesions. In fact, the two major classes of chromosomal lesions are recognized and processed by the two individual pathways at the early phase of homologous recombination. It follows, therefore, that homologous recombination events are occasional reflections of the continual recombinational repair, made possible in cases of natural or artificial genome redundancy.
Finding Inversions
Author: Molly Schmid
Source: The Lure of Bacterial Genetics , pp 227-231
The author's project at University of Utah was an interesting mix of bacterial genetics and evolutionary biology, which aimed to gain understanding of what evolutionary forces would preserve the order of genes in the Escherichia coli and Salmonella enterica serovar Typhimurium genomes over the 200 million years of separation between the two species. The basic idea was to change the genome and see what happened, and the mechanism to make the chromosome rearrangements was to use Tn10 as a portable region of homology. A selection/screen was designed to find inversions, based on the his-203 deletion selection that was first used by Ames, Hartman, and Jacob in 1963. The flurry of subsequent tests—nontransducibility of both the his operon and the auxotrophy—had been predicted on paper, and all quickly bore out to be pretty much true. With the confidence of knowing that inversions would arise from the his-203 screen, the author went searching for nonauxotrophic inversions by using the non-transducibility of the inversion breakpoint to identify them. This was a massive cross-streaking exercise, but much less work than the initial auxotrophy screen. The only problem was that the tools to characterize the inversions, to figure out the location of the second end point, were going to be difficult and ultimately not very satisfying. What emerged from the initial mess of crosses was a method to map the end points of the nonauxotrophic inversions, which was really unexpected and amazing.
Barriers to the Formation of Inversion Rearrangements in Salmonella
Author: Lynn Miesel
This review illustrates the logic style that Roth taught in investigation of chromosome rearrangements and highlights the interesting findings. A few explanations were proposed for the rarity of inversions: inverse-order repeats occur infrequently in the chromosomes of these organisms; inversion rearrangements may cause deleterious effects that cause a selective disadvantage; and mechanistic constraints may prevent the formation of the rearrangements. Inversions were frequently recovered among recombinants when the repeated sequences flanked certain chromosome segments, termed "permissive". The author focuses on the Roth lab's approach to studying the barriers to inversion of nonpermissive chromosome segments in S. Typhimurium and will correlate findings with outcomes of investigations in E. coli. Alternatively, barriers to the recombination events required for inversion may block formation of the rearrangements. The crosses yielded inversion rearrangements at frequencies expected of two-fragment transduction events. The role of DNA replication is considered in the homologous recombination events that form inversion rearrangements. Investigation of inversion formation by homologous recombination in E. coli found that replication pausing at inverted ter sites is not the only explanation for nonpermissive and restrictive intervals. It remains to be determined if other barriers limit inversion in Salmonella. The findings summarized here highlight topics to investigate regarding the mechanism of inversion formation and barriers to this process for nonpermissive chromosomal segments. The role of DNA replication in inversion formation could be examined by testing the outcome of placing blocked ter sites within permissive arcs of the chromosome.
Yeast Cytology, 1950 to 1990
Source: Yeast Research , pp 60-75
This chapter summarizes selected and somewhat disparate advances made in yeast cytology after about 1950. Northcote and Horne disintegrated their baker's yeast mechanically, and after centrifuging, mounted the cell wall fraction in polyvinyl formal films. The yeast walls proved to be stratified: after acid hydrolysis, chromatography showed that the outer layer was mainly mannan-protein; the walls contained 29% glucan and 31% mannan, previously reported for yeast cell walls as 13% protein and 8.5% lipid . It was in 1956 that Necas described the formation of some protoplasts or spheroplasts amongst spontaneously autolyzing S. cerevisiae. In 1989, Hartwell emphasized the relevance of this work on the cell cycle for the prevention of mammalian cancers. Chromosome behaviour in meiosis is well characterized from cytological and genetic descriptions but little is known of the underlying molecular mechanisms, largely because no one experimental system has been developed to support an integrated application of modern cytological, genetic, and molecular biological methods. A thorough account of the amount of work published on yeast cytology in the last 50 or so years would occupy several volumes; in addition, no account has been given here of advances made since 1990, which involve such innovations as the green fluorescent protein reporter system, confocal microscopy, and flow cytometric analysis.
COLOR PLATES
Source: Microbial Metal and Metalloid Metabolism
No descriptions available.
Studying Simple and Complex Traits Using Pedigrees Produced From a Large Database †
Author: Cheryld L. Emmons*
Citation: Emmons C. 2010. Studying simple and complex traits using pedigrees produced from a large database † . 11(2):156-157 doi:10.1128/jmbe.v11i2.156
Students in genetics classes become familiar with interpreting pedigrees for modes of inheritance of single-gene traits. Non-majors' genetics courses often have students produce pedigrees of their own families. This exercise provides students with an extensive database, including over 50 families, from which they are asked to produce pedigrees as evidence in support of or refuting suggested models of inheritance for simple traits. Students are also asked to propose modes of inheritance for complex human traits based on pedigrees that they produce from the database. The people in the database are real and include their own families. This makes the exercise more real and personal for the students.
Initiation of DNA Replication
Authors: Alan C. Leonard, and Julia E. Grimwade
Citation: Leonard A, Grimwade J. 2010. Initiation of DNA Replication, EcoSal Plus 2010; doi:10.1128/ecosalplus.4.4.1
In recent years it has become clear that complex regulatory circuits control the initiation step of DNA replication by directing the assembly of a multicomponent molecular machine (the orisome) that separates DNA strands and loads replicative helicase at oriC, the unique chromosomal origin of replication. This chapter discusses recent efforts to understand the regulated protein-DNA interactions that are responsible for properly timed initiation of chromosome replication. It reviews information about newly identified nucleotide sequence features within Escherichia coli oriC and the new structural and biochemical attributes of the bacterial initiator protein DnaA. It also discusses the coordinated mechanisms that prevent improperly timed DNA replication. Identification of the genes that encoded the initiators came from studies on temperature-sensitive, conditional-lethal mutants of E. coli, in which two DNA replication-defective phenotypes, "immediate stop" mutants and "delayed stop" mutants, were identified. The kinetics of the delayed stop mutants suggested that the defective gene products were required specifically for the initiation step of DNA synthesis, and subsequently, two genes, dnaA and dnaC, were identified. The DnaA protein is the bacterial initiator, and in E. coli, the DnaC protein is required to load replicative helicase. Regulation of DnaA accessibility to oriC, the ordered assembly and disassembly of a multi-DnaA complex at oriC, and the means by which DnaA unwinds oriC remain important questions to be answered and the chapter discusses the current state of knowledge on these topics.
Modeling Genome Size
Source: Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition , pp 206-211
The activity described in this chapter uses models to show the relative sizes of an Escherichia coli cell, the E. coli chromosome, a typical plasmid, and a gene. The models demonstrate graphically how much longer the E. coli chromosome is than the cell. Calculations included in the student questions use the analogies of letters in a book and miles of railroad track to suggest the size of the human genome. DNA is stored in cells in the form of chromosomes and plasmids. The amount of DNA required to store the information necessary for making even a simple organism, such as a bacterial cell, is very large. One of the wonders of biology is that cells are able to store and access the great lengths of DNA needed to encode their hereditary information. It is capable of high degrees of folding and coiling, an essential feature for packing it into the cell. Although the degree of folding required to fit the DNA of E. coli into the bacterium is impressive, the folding necessary for packaging DNA into a human cell is even more remarkable. The DNA models consist of appropriate lengths of thread, yarn, or string. At the end of the activity students will able to list some differences between the E. coli and human genomes.
Source: Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition


text:
A Family House Flipping Business Truly Focused on Serving Homeowners With Laura Casey
Laura Casey shares all about her family's house flipping business and how they truly care about figuring out and solving the real problem the homeowner in a distressed situation is facing.
I don't buy into the notion of "you shouldn't mix business and family/friends." Wouldn't you rather grow and do great things with those closest to you?
Laura's background and how it perfectly led her to her current role (even though she may not have realized it IN the moment)
What their business/team looks like
Who takes on what roles in the business
Challenges and gifts of working with family
2. Follow That Flip! Follow me and 2 local students as we flip a house together!
You're listening to the flip houses like a girl podcast where we educate, empower and celebrate everyday women who are facing their fears, juggling family and business, embracing their awesomeness and wholeheartedly chasing their dream of flipping houses. Each episode delivers honest to goodness tools, tips and strategies you can implement today to get closer to your first or next successful house flip. Here's your spiky haired breakfast taco loving host house flipping coach, Debbie DeBerry.
What is going on you guys, it's Debbie here. And I hope that whatever you're up to today, it's an easy one. If you aren't already on the waitlist for flip your heart out, which is my real estate investing retreat, where we will meet house flipping. We're having it in September, in Austin, and it's going to be unlike any other real estate investing event you've been to guaranteed, no sleazy selling just good old fashioned connection and creation and how to have a bigger impact on others lives and your own. Okay, so go to flip your heart out comm and join the waitlist because we're about to open up sales to non students. Because right now it's only open to students. Okay, now, let's talk about today's episode because I adore this woman. Her name is Laura Casey. She lives here in Austin now and she and her son and daughter in law have an awesome business that is about serving home sellers. And it's just it's so freaking inspiring. I love what they're up to. It feels so good to meet like minded investors, who aren't just out to make a quick buck while they trample over everyone in their way or on their path. So yay, for meeting more awesome like minded people. All because of my little monthly meetup that I started in October. If you remember, if you listen to the episode with Lindsay Hinkle a couple of episodes ago, I also met her and her husband for the first time at that first meetup. In addition, I met Laura and her son and daughter in law, and I'm telling you, and ah, just awesome lives, awesome people. And I love this conversation that we have. All right, we are talking about their team. We're talking about her background in the roles each person plays. We're talking about mindset. We're getting into the nitty gritty here, folks. So stay tuned. You don't want to miss this one. All right, see on the flip side, how about let's start with you giving us a little bit of background on you personally.
Laura 03:17
Okay, awesome. Um, my background is I've pretty much always been in some sort of design or construction field and that truly is where my passion lies. And I went to the University of Kansas and studied interior architecture. Graduated they were just kind of morphing a program between the School of Art and the School of Architecture. So it was this really cool blend of that they were just starting out so that was really fun. Didn't did mostly like commercial projects through school. And then shortly after that went to work for one of my professors in on the Plaza in Kansas City worked for him for a couple of years doing some commercial stuff. And then after that I headed out to Arizona with it just decided I think I should go to Arizona so just headed out to Arizona and the only thing I couldn't really find was at Home Depot.
That's amazing. Okay, I don't know about you.
Okay, amazing is the word but the time it was a good thing. They were starting like an interior design program where you would go in people's homes and solicit interior design services along with all their products. So I kind of doing design, although obviously much more residential. But the good thing about it was they also taught me how to do Kitchen and Bath design. So I learned the 2020 program ended up selling Kitchens and Baths and running their design department through Home Depot. Nice. So it was, you know, probably not the place I would have loved to have been. But it was a really good experience and a bit of a springboard and my parents, Arizona, and then one of the contractors I worked with eventually offered me a job and sold me away and he built big Custom Homes in North up in north Scottsdale in Arizona. I worked for him for a while. And what were you What were you doing for him? Um, you know, it wasn't really glamorous, and I didn't you know, really fulfilled there was a lot of office work and gotcha, a little design. Yeah. Oh, because it was just consult just me and him. And so he was out there selling and doing a project managing and I was kind of handling all the office stuff. Gotcha. Not my happy place to be sure. Yep. Um, then I went to work for a family run interior design business, they did cabinets and sold to smaller builders, and I mean, did cabinets, countertops, all that design coordination, that kind of stuff. And again, that was just a really good experience because I started doing like really high end custom cabinetry, things like that. And then they got bought out by a division of Home Depot, Home Depot. This CTI creative, and they have like a lot of small mom and pop shops, you know, tile shops, cabinet vendors, things like that. They bought everybody up in Arizona and created this giant, like 400 person company. Wow. And that was leading into the crash of a weight. Okay. Oh, that thing pretty much dissolved. Everybody I knew we all just kind of scattered like rats in different ways, because everybody lost their jobs. At the time, I lost my job. My fiance at the time, lost his job. We lost our house, and we lost everything. And we weren't. But yeah, went through that whole Arizona was hit really hard.
I remember hearing about it. Wow.
So like, everything shut down. And we all went and just found random jobs for a couple of years. And then everybody started to resurface. So then when that was all over, I did a bunch of weird jobs and interim, and then I went it went to work for a, like a high end, full service remodel company, or really, okay, in Arizona, and did everything from meeting with the initial clients going out to the home and doing all the the measuring and the 2020 design work, specifying all the products, writing the contract, doing all the installation, Docs, everything and then I would hand it off to a project manager and I work with him to see the project through. Okay. So I did that. And that was really great, because it was kind of a small company had a real family feel that everybody was under one roof. So if you need to, you know, your payroll department or your project manager or your lead designer, like everybody was there, all the material was there under one roof. But I learned a lot there. Yeah, it was really good. And every project was completely different. I got to work with great clients, I really got blessed with a lot of really great clients that gave me a lot of creative freedom and have really nice budgets. So it's a great, glamorous background story. I don't feel like ever have really like been in the pocket loved what I was doing felt like I was in the right place. But I feel like now I can look back and see it was all just kind of stepping stones good experience, that kind of stuff.
Totally. That's so like, that's just so true. When you're in it. You're like I don't get any i don't i don't get the purpose of this. I'm I don't know and then later you can like it's always nice when you can look back and be like, Okay, I get it. Yeah, didn't have to be that way. But okay, I get it.
And I'm sure We might talk about it. And you and I've talked about it before, I also think that a lot of that kind of just contentment was staying in my comfort zone. You know, I was, I was perfecting a craft and skills, but it wasn't fulfilling, because that would have meant taking more risks.
yes, exactly, let's talk about that! um, I just read a quote, dang, and bombed that I'm not remembering who originally said it. Um, I read it this morning. And it's basically, like, we get that, I get the notion of, you have to get outside of your comfort zone, if you're going to grow and to to, to advance in any way you have to get outside of your comfort zone. And the quote was something like, basically, if you feel like you're stuck, and you look at your day to day, and you are not uncomfortable, and you are not doing anything that feels stressful, or causes anxiety, or it feels uncomfortable, then you're probably plateaued, but you probably not advancing, you're probably not going to basically reach whatever the goal is what you're after. And it's like, it's so true. And it's just, it's cliche, and it's so true. Equally,
I 100% agree, in fact, you know, you sent me a couple questions to think about and I took some notes, and this morning, I even wrote one down because when I was at the gym yesterday, you know, again, like, I love the gym, I go to I love the routine. I love that. I can measure how much weight and now I lift this much weight so I can really see where I'm changing. But even with that it's very easy to get in my comfort zone at the gym. Yes. And one of the things the trainer said yesterday was like, you know, in the uncomfortable is where it happens. Yes, our hearts are pounding out of our chests. Change is not happening. So he's got to write my favorite quotes is like, you know, getting being comfortable being uncomfortable or?
Yeah, I mean every day I'm thinking about it. And I know you do orange theory, and I recently started doing peloton cycling workouts I don't have a peloton bike I actually rent a site. Uh huh. in Austin, there's a company that rents like professional grade, like gym grade bikes, okay? for like, I think it's like 75 bucks a month, because I just didn't want to fork over the 2500 bucks. I didn't know if I was going to. I didn't trust that I'd stay committed to it because I'm a runner. But I had injuries that I can't run anymore. So I was like, Alright, we've got to do something. So let's try cycling. And I'll tell you, I want it I'm totally in love with it. Um, but the peloton coaches, like every single one of them. I'm like, they'll say something I'm like, Yes, that's totally true. Yes, get uncomfortable. And but it's like it you can you can take it into every area of your life. Like it's, it's just totally true. out. And I'm the same way I'll be like, Oh, this workouts easy. I feel like I'm working out but you know, and all I have to do is crank that stupid resistance up one more notch and it would be a totally different workout. But do I want to do I want to be that uncomfortable today. It's like you're in charge. You get to control it
That would be a good t shirt for you, crank the resistance up one more notch.
writing it. writing it down
Well it's been about this because I have felt really anxious and uncomfortable for like the last two weeks. It probably started when you asked me to speak in front of your group.
to the uncut that was getting uncomfortable. But yesterday I was kind of doing some soul searching and realizing that we're in this very uncomfortable place with starting our new business and yes known and that was probably a lot of the feelings I was having and that I just need to be okay with that because the growth is happening like I'm experienced. thing that right now, but when you're in the thick of it, it's seen how badly you want to go back to your comfort zone.
yes, yes. Because you know what to expect?
So let's talk about that. Okay. So, when, okay, right now you're in business with your son and daughter in law. And when did that happen? What year did that come together?
SO, that was just 2018. we moved out here Mother's Day weekend of 2018 with a big faithful leaf. And do you want a little backstory on that or no?
Yeah, yeah, absolutely
Okay. Um, so my son was working for a home buying franchise in Arizona. He was working for an owner and he was going out and basically being the buyer meeting with buying the properties for somebody else. He was doing the hardest part. I think, our end he was really, really good at it. Yeah. And people quickly realize how good he was at it. So other people were calling him and trying to get him on board with them and their businesses. the meantime, the man who owned the franchise in Arizona had kind of a dormant franchise up in Portland, Oregon. And so he said, Hey, would you want to go up there and check it out and see if you could make it work up there. So my son got the opportunity to go up there. And we all tagged along for a summer up in Portland, and I forgot about that, but my daughter in law, my mother went with us. So my son was there with three ladies. I love me all summer. But that's when I had been going on some appointments with him in Arizona. In my free time, you know, really, I was really interested in what was happening in his life and his world. And me. So if you let me tag along, I did some trainings, the owner was fine with me, going out with him on appointments, and we actually got quite successful going out together. And so I did that when we went up to Portland, and then started doing some of the CRM and running comps and just getting my feet a little bit more in his world. And then when he came back from Portland, he and his wife realized that they really wanted a change of pace, and we're looking to relocate. So they started looking at, you know, Nashville, and Boston and Kansas City and all these other places where there were franchise opportunities. Got it, and company. Um, during all of that, I went through a really devastating divorce. That person devastated our entire family. And I was just really lost. You know, I was in this job working for a remodel company, but not really satisfied knew I really didn't have anywhere to grow with that business. Yeah. And they mount to our weekend, they both make really quick decisions, which I don't like came back and said, We love it. We're gonna run a franchise out there. And we're moving and why don't you come with us? It was a really quick decision for me normally have to start worrying, anxiety and sweated by some tears. And you know, they used to drive out here, so that all happens, but But, yeah, that yes, like, why not? What have I got to lose? And this may be the big opportunity of my life and I was in my 50s. And so early drove out here found the smallest, cheapest apartment in Austin, which is not easy to do. Right? off, it's not easy.
I was gonna say, it's probably not cheap.
Cheap is a relative term. Yeah, and we came in on Mother's Day Sunday, and we went out on appointments Monday morning. So Bill with the franchise, okay. And then we did that for about a year and a half, my son was incredibly successful with that he ended up running the whole Austin region being in charge of all the franchisees in Austin. Um, and then we just decided over time that we wanted to do something a little different, that it was no longer serving our needs and that we really wanted to create this for service centered message and a healthy home sellers, but they weren't being served as far as we could see. Yes. So that's kind of the creation of the new company and one really specific message and we, one of our taglines, as we put people over properties, yes, because we started to see that sometimes no amount of money, solve their problems, right. So even though it's a really competitive market, and, you know, you have multiple offers, and investors are everywhere, a lot of times, it didn't solve their problems. And so we could really sit down with them, and get to know them and put a game plan together to solve whatever problems they were facing. Things like, you know, they might have a probate issue but not have money to deal with the legal fees. Right. Or they need to they were facing a foreclosure needed to get in a new place, and literally didn't have a penny in their pocket to pay for a down payment on an apartment, or truck. A lot of needed a lot of patience, you know, a really flexible, closed date that could change depending on their circumstances. Or they needed to lease back the property after they sold it. And then they needed the cash in hand, but still needed to stay in the property. So we could just get really flexible and super creative, and really just hold their hand through the process. I don't know that that's really the question you asked me.
no, yeah, it is. One of the things that I love about you guys, is like integrity and serving first, and not being so pocket driven. And that's one of the biggest things when I was exposed. So I wanted to be a real estate investor starting in 2003. And I didn't know what I meant by that, you know, I, I had all these people telling me, you've got to buy short sales, you've got to invest in in tax liens, you've got to buy foreclosures, you've got to do these. And I'm like, so all of that kind of sounds kind of like pushing paper. To me, it doesn't sound like a relationship kind of connection, kind of business. That is the connection is so important to me that every other strategy I tried, I was like, well, this sucks. Maybe I don't want to be a real estate investor, because it this is what real estate investing is, this is gross, it doesn't feel good. Um, until I started flipping houses. And then that's when I was like, Oh, my God, I can actually make a big impact on local communities. And I can serve people in a way that is meaningful to me. Right? So I love that you guys lead with your heart, because it's one of the things that is harder for me to find. That's important to other investors. And it's like, it just makes me want to hang out in Daniel's bubble all the time.
Well, good. Good, because we have that, you know, that in common. And I think when you spend some time with people, you can very quickly see like, what their motives are and what they're driven. or fat. And we have tried very hard to create a tribe of like minded. Like, right? Oh, yeah, it's it's important. I mean, it's like, I don't know, I don't know,
if another way works. For some people. It just doesn't work for me. And so I'm glad that we found each other. okay, so you guys moved to Austin, and you recently, you're transitioning into your own company right now. And so it's the three of you. What do I want to talk about this piece, because I think it's really great to learn more about what a team can look like, and really how you guys can support each other. Because it's really, there's not a person that can do every single thing necessary in this business. As well as they possibly could, like you can't do everything 100% so I think it's great to hear how you guys are navigating that What does your team look like?
Sir? So we actually know how for the RAF. Um, but so Max is my son. He's 26 headed to 27 and He's the owner and then his wife Angie. Then we now have a director of operations, which is Kara, as well. So one thing I have to say is, Max's spent a lot of time really researching and learning about personality styles, communication styles, teams, structures and things like that. What kind of roles people will play. And he spent a lot of time talking about those things and figuring those things out. Because we really feel like, you know, just like with a house, that foundation is critical, and I think, just kind of don't do any part of that process. I mean, they may say, Oh, I need somebody to handle my billing. Let me find somebody to do my billing. Let me you know, it's more of like, what, what is the task? Yes, we really focus on who is the right person to handle what parts Max's the visionary, he moves at a very high rate of speed. Like big picture. Definitely high communication skills. I'm always forward thinking, things like that, you know, you're not going to see max sitting in the office doing paperwork all day. Yeah, um, he's not a huge networker. Angie is very, let's see, I made actually a couple of notes here. Let me just take a look. But um, yeah, so Max's, you know, Max is also our closer, so he acquires all the properties. He's the front man talking with the sellers, because he just has such a high communication skill. Sure, um, Kara is the most logical, detail oriented, she loves processes. And, like, get that girl in front of a binder or a spreadsheet, and she is giddy.
Oh, my God, I need a Kara.
Everybody we needs one. she would puzzle that is our most recent addition. And, and she's just kind of completed. Wow. Um, Angie is Angie is extremely creative, high design. But yet, she's very introverted. So she likes to do things, you know, behind the curtain. doesn't like to be out there dealing directly with people. So she's great at keeping us on budget. She is on task, sending us reminders, sending out emails, text messages, things like that. But she's private and reserved, and creative and introverted. And so she gets involved a lot in the design process. But she doesn't like the project management because that is the communication. And then myself. I was looking at your questions. I was kind of laughing because one of your questions was kind of like, what does my role look like? Uh huh. Oh, it's literally it changes every day. But I kind of got a chuckle because I do have an official title at our company. And I have to read it because it's so long. And my official title is director of creative solutions and connections. I love that. I'm also a realtor. But I love that you spent a lot of time even thinking about what our titles were because they were very personal. And we wanted them very specific. So I know you know me a little bit and it's hard to believe, but I like meeting people. Now, I love working with people. And like a really good day for me is if you were to come to me and say I need a cara. And I were to say I have a Kara for you like that. makes my day to be able to care
Yeah, you're totally a connector.
Yeah. I'm Maxwell say I'm the who of the company. So he, we need a probate attorney. And I have to go out and I find a probate attorney, or how can we possibly solve this? And we'll brainstorm some ideas. And I'll say, I'm going to reach out on social media I'm going to call so and so I'm going to call Debbie, you know, who do I know who can I reach out to that kind of thing. So I do think that as we're learning and growing and developing this company, because we really We don't know anything. So people who know stuff like you. Yeah, that's kind of those are kind of in general, our roles. Everybody really has a lane that they see. And it's a lane they like to stay in. So Matt, airy good about, where do we want to spend our day? How do we want to spend our time? What makes us happy? What energizes us, gives us joy, when we feel like we're contributing to the whole, because yeah, he sits me down to do paperwork all day, I'm gonna be miserable, right? But you sit down, you tell Kara, she has eight hours of paperwork to do and she gets her coffee and her treats and she's excited.
I'm happy doing pretty much anything with coffee and treats. Okay, so seeing how your team works? About how many projects are you guys working on at a time? And I know that might be different now. Because you're, you're you're kind of you're launching this new business, because you're not affiliated with the franchise anymore.
Um, you know, it does vary quite a bit. But I'd say I mean, we're not like a hot high volume kind of business. I mean, we might have two or three checks at a time, and we don't fully remodel everything. So when one or two big remodels in a year might do, you know, whatever, 5678 what we call light rehabs, that are painting carpets, kind of thing, and assign a bunch of them. Yep. You know, double clothes, things like that. Just buy them and get rid of them. So our business is quite a mix of things. And going into the new business, our plan in the beginning is probably to do a lot more assignments and a lot more, you know, quicker transactions and not take on big remodels right now, because eventually that's a component, we want to build our business to be able to handle our own remodels, but that will come with time.
Gotcha. So really, when I think of you guys, I think your main and tell me if I'm wrong, but I think your main business, your main focus is solving sellers problems. And Is that it? Okay! So the more sellers you can help, the better the bigger impact your business is making, that you guys want it to make. So it makes sense to me that you're not rehabbing. 80 something houses a year, um, because that's a different it's a different focus, like you're still helping those homeowners, but you can wholesale you can assign contracts, and you can help more people, basically yeah.
And, you know, the designing part of it again, I feel like you know, I know I'm totally biased, but I feel like Max is very good to us in that way as far as being a business owner and a leader of a team because he recognizes that Angie, and I have to have that design component in our life. Right? I'm so right he you know, we'll take on projects like that just to keep us happy and satisfied. Yeah.
I was gonna I was just gonna ask about that because yeah, like as somebody who really likes the design piece and who I know for you guys like that for you and Angie, that design that creative expression I get it that's important in that field that it's hard to find another outlet for that unless you know you guys did consulting work for other people which mean there are pros and cons to that so
we're joking The other day I said when we were in front of your group I said that's why I'm reaching out to people like yourself and Lindsey that you interviewed and some of your other people that you coach and mentor I'm like I need to come see your project like I just need to be on site.
Just need to smell that drywall. Thought ask it a little saw that's in your loans to see a good dumpster and smell some bad plumbing and.. Oh god, that's hilarious. Um, so when it's interesting That you and Max were kind of doing this. Was he in the same city? As you when you were doing all the remodeling work and stuff?
When I was working for the remodel company?
Yeah, when you were doing all the design work and remodeling and stuff, so I guess his focus though, at the time was a buyer for other. Okay, because it's like, you guys were so parallel. It's interesting that it took a little bit longer for you guys to join forces, but it makes sense because you were working for someone and he was working for someone else.
Yeah, and his the guys that owned the franchises that he was working for were mostly into wholesaling or doing really light rehab. So volume type business, and auto company I worked for was definitely not investor friendly. You know, as far as with investors and things like that. I wouldn't have made sense at the time to work together.
That makes perfect sense. So when you guys I mean, I guess struggles that that you have with this new business, like mentally like, Are you worried it might not work or that I don't know. Do you have any mindset struggles right now with with this launching of the new new business?
Uh, yeah, absolutely. I am on cry anxiety alert. And Coronavirus.
Is that? Is that what I see? I see like a red flag on my desktop. And this led me Hello, Laura. Casey is on high alert. Along with Coronavirus
You can get an alert on your your watch on your Apple Watch. Hello, anxiety at any point in red alert. Um, yes, the answer is yes. And, um I think it's one of those things, though. Um, so you used to be an athlete, right? You're still an athlete. But you grew up? sports, right? Yes, absolutely. I think it's, I didn't so um, but I think it's one of those things when you make a decision. Feel like, spiritually and emotionally and every other way. It's what you're supposed to be doing? Mm hmm. I don't feel like I have any other options. Mm hmm. Every day, you just make it work. So, yes, there are always those thoughts of anxiety and self doubt. And what in the world did we get ourselves into? And we thought that the times I mean, packing up a car and driving to Texas, became a realtor and literally not even knowing what street I was on. It talks about this people are like, Oh, you know, this area? You know, this is, you know, oh, you know, I heard I just got here yesterday, just pulled up and zona are straight and on a grid.
And in Austin, the same street can have five different names, depending where you are on it.
Yes, I would tell my friends. It's like driving on a spider graph. Haha. That's always that's always there. You know, you always question. You know, we question even yesterday, we had a meeting, we were meeting at nine o'clock last night, and we were talking about you know, what everybody else is doing? Yeah. How are they getting leads? How are they door knocking? What's their message? Why is everybody getting these properties? And we're not, you know, and that stuff usually doesn't last very long. Get out of our little circle, but is there? Yes. But I do think that what we have going for us is the vision. Yeah, we are all laser locked and focused on what we're trying to do and what our messages Yeah. Just it drives you as I would imagine as an athlete when you are focused on the result or the end goal or the theme. You it makes you get up and do things you don't want to Do?
Yes, absolutely. Hundred percent. Absolutely. So that, um, I want to know, I guess it's like, I want to know what the difference is. So, because there are people who want to say they want to flip houses, and they aren't, or they, they don't have that. That commitment that look, you, whatever you choose for your goal, whatever it is, if you keep showing up and you keep trying, you will achieve your goal. The only difference is the only difference between someone who achieves their goals, someone who doesn't is the other person stop trying, they gave up. And usually they gave up like, a centimeter away from getting everything they wanted. So but what is that? What is it in in you? Is it just is it not just because it's a big one? Is it that commitment to the the message, the mission and the message
It might be a combination, because there's definitely that we have spent a lot of time and, and gotten a lot of bumps and bruises. figuring out exactly where we're supposed to be. And we feel like we have figured out the message and we figured out who we need to serve and how we need to serve. But we're still trying to figure out all the logistics and how to make that happen. You know?
I know that Yeah, sure.
Yeah. So, um, so I definitely think that's a part of it, having that vision, feeling like you are doing what you're meant to do, whatever that's you. But sometimes I often think you really need to look at what your strengths and weaknesses are. Or downfall. You know, I have a lot of people come to me say, Oh, I want to flip houses, you know, and they know how to run numbers. And they know comps and they know, but maybe they aren't comfortable talking to people or networking, or designing or, like you said all these other things that people are trying to do on their own. So I think it's, I feel very fortunate that I'm part of a team that I don't think myself because I don't know that I would feel the same way if i i'd like to think that if I had my vision, I would be that driven to get up and make it happen. But I very often and fortunate enough that I get to rely on other people. And when I'm, you know, maybe having a rough time, there's somebody there to help me out or pick up the slack or to do some course correction or whatever the case may be. So when you're when you're doing it alone, it's great to say, I think if you have the vision, you just get up every day and you make it happen. But I think especially when everybody has their strengths and weaknesses, you're up and automatically want to focus on the things that you like to do, right? Less you do have that ability or drive or whatever it is that makes you do the things you don't want to do. Right. And I think that's where a lot of people end up quitting before they're there. Because they're need to enroll other people in their possibilities. And even if it's, you know, a really formal team. You know, you have to have your tribe you have to have you have to have other people you can rely on or reach out to. But you're all alone, I can easily see why people give up or fail. Yeah, I totally agree with that. Yeah. Yeah, it's hard, hard to do it yourself. It is it is and it's, it's not fun putting yourself out there. It's not fun. You know, it's not fun. Showing up and not seeing results as quickly as you want. It's not fun, not feeling like people are listening to you. It's not fun. But then that one time that you are able to help someone it's like, holy crap, this is what I'm living for, like this is it. This is great. I was able to help this homeowner, like it's when it clicks. It's great, but the in betweens can be a struggle sometimes. So I think it's great to whether you like you were saying whether you formally build a team that you work together closely every day, or even in selecting the realtor that you're going to work with, or the lenders are going to work with all of them are part of your team and they are going to bring a different kind of energy into into your world, your professional world and Your personal world? So do you want that energy to be? You know, do you want it to add to your day do you want it to take away from your day. So it's, it can be very easily go either way. And I think regardless of whether you're building a team of, you know, working with your family or close friends every day, or it's just people filling different roles, it's important to, it's important that your visions align, and that your, your focus on the same thing, because otherwise, it's just going to be so much friction, and you don't have to go it alone. And you have to have people around you, that are supporting you, you have to like, that whole notion of self made, I think it's total BS is Oh, it's one of the terms that it just makes my it makes my blood boil, because nobody is self made. That whole notion of self made is just wrong. It's not there's nobody has achieved anything on their own. There's, it's impossible and I think there's a lot of that out there, and social media and the world, this idea of anybody can achieve any goal they want. And they can do it all on their own. If they just get up at 5am and have their morning routine and do XYZ and I agree with you, I you know, even people that say they've done it that way, that's that's not the case at all. There's hundreds of people that have been on that journey with them.
Right, the people who I knew when I was three years old, right, like they have impacted my life in some way. And they have made me who I am, like, everybody has a role. Um, yeah. I think that the DIY, like, I get it, I love it, the whole DIY movement, it's great. And it also presents this whole thing of, if you aren't doing it on your own, something's wrong. Because you can just do it yourself. and expect all the great things and it's not like, I feel like it's kind of it's a little bit toxic. The messaging around it, it's a little bit toxic.
I like the whole, it's like I related to the whole body image thing. It's like, you know, it's kind of sending this message that it needs to be perfect. It needs to be polished, it needs to be, you know, quick, that kind of thing. And it's like that's, that's not the way this world works at all. It's messy, and there's a lot of failure. And there's been a lot of support and all that crazy, you know, crazy stuff. That's life.
Yes, exactly. Yes. It is messy. It just is. It's not it's not going to be pretty nerve. It's going to be pretty. If you want pretty. I don't know. Yeah, I don't know. I don't know what to tell you. Yeah. alright, I'm just being mindful at the time. Um, is there anything, this has been such an awesome conversation, by the way I knew is going to be awesome. And it's even better than I ever imagined.
Is there anything that you want to touch on? Or say, as we wrap up the call?
Um, no, I think the only other question that you asked me that might be worth just saying a few words about as you had asked about, you know, are there any points of contention or things I've learned about myself or that we've learned, like, in working together as a family? And I definitely think that's a good point. Because it is, you know, it is tricky. Um, we have a big joke in our family, because my son max will call me Laura, if it has to do anything to do with business. If it has anything to do with personal and he literally can flip a switch. I mean, it will be the same conversation and he'll be like, okay, now, Laura, I need you to do this tomorrow. And this is what's on the calendar and blah, blah, blah. And by the way, Mom, what time should we hear? Dinner. He's able to really, things in their proper place
That's impressive man, that's compartmentalization. Right there. Max.
Not only working for family, but like, you know, I'm a mom working for my son. So it can get really tricky, but I think the one thing I've learned is definitely, you know, patience. Um, I think we've all had moments where we really have to check our ego at the door. Yeah. Realize that everybody is doing their best. And yes, little love and patience and compassion and support. And I've had times where we have to step in and handle things like a business and like work came together and be able to have really sometimes difficult and frank conversations or change up our roles because something's not working. And Max is really good about not playing favorites. You know, there have been times where he's had to put me in a position maybe Angie wasn't happy with and vice versa and times where he's had to take something off of somebody's plate and hand it to somebody else. And it's just business. And so you really do sometimes have to compartmentalize that. But at the end, they'll family you still love each other, you know, part of the family now, so are the same way. But
yeah, you know, that's another thing that I often hear, um, don't miss don't mix business with family or friends. And I just I don't bite into that at all. I don't know who else you can trust more. I think you have to be cautious of anybody you go into business with whether they're a friend or not. But though some, there are some people who are just make blanket statements in general. And that's certainly one that I hear often, especially in this real estate nasty world. Do not go into business with friends or family.
No, man. Yeah. And I think you just, I think for us, when I look back and think of how we weathered some of the difficult stuff really is you'd have to be okay, communicating, you have to be okay, in your mind, but being very compassionate and mindful of the other person, you know, just like you would in any relationship, but I think even more so in a family.
It all comes down to communication. Everything always comes back to communication. everything.
Alright awesome. You're awesome. I love you to pieces. I love your family. I love your family's business. I love it all. And thank you for sharing it with us. Because I think there's just, there's so much. There's, there's just a lot of insight and so much to learn, people could learn from this conversation. So thank you.
You're welcome. I love you too. And I appreciate you getting me out of my comfort zone and asked me to do this to shape the opportunity. On a really heartfelt note. You know, we are very, very faithful people. And this whole journey has been, we feel like driven by a divine power. And I know you are part of that you were dropped into our laps. And I know that was no coincidence, no mistake, and I hope that we are BFFs forever. And we can go build a super awesome container home community.
Oh yes, yes. And I feel the exact same way. It was not like view you guys walking into that first meetup that I thought maybe nobody was going to show up to. Um, that was awesome. I was just like, oh my god, these are my people. And I had no idea they were they were here. I had no idea you existed. So I'm super blessed.
blessing to be there. I feel blessed to be a part of all of it. And I'm just so excited for your group and your people and for you and I love being a part of it. So thank you.
I appreciate you. I hope you have an awesome rest of your day. And I'll see you for tacos soon. Yeah, I'm gonna go do stuff I don't want to do today. Yes, I love it.
Me too. Let's do it. YAY cold calling.
Yay. Oh, that's right. Oh, man. That was something I wanted to talk to you about. Okay, we're gonna have another conversation soon. Okay. Bye
Was that awesome or what? So, so good. Ah, man, I love her. Okay, that's it for this episode. Until next time, go out there flip houses like a girl. Leave people in places better than you find them and make it a great day. Bye. Oh,


I just stuck with my cutout series "Four seasons" so I dedicate my time to "Cat with attitude" project. This is a paper cutout I made while observing my cat playing with a ball. It was cut out in white Canson sketching paper, the background is black Canson cardboard.
PS: Sorry for the scan quality - I just couldn't make it any better.
Ok, I really like the simplicity of the cat figures. On that background they stand out very well. I also like the panel effect. You certainly have a good eye for cutting.
Sass, Wally's Mom - thank you very much for your kind words.
I saw this in the projects and just loved the positive and negative space as soon as I saw it.
This is terrific Lady Grey! Excellent cutting skills. I really like the subject and composition on this one. Thanks for sharing.


text:
Volkswagen presents Warren Miller's "Face of Winter"
Thursday, December 27, 7:00pm
Tickets: SOLD OUT
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SOLD OUT- Thank you and see you next year!
Loving the pure joys of winter is something we have in common with the late, great Warren Miller —who helped create and capture the magic of skiing.
"Face of Winter," the 69th installment from Warren Miller Entertainment presented by Volkswagen, will bring new and veteran athletes together to pay tribute to the man who started it all. Visit some of Warren's favorite locations, from Engleberg to Chamonix, British Columbia to Alaska, Chile, Iceland, New Zealand, Washington and more.
Watch as world-class athletes like Jessie Diggins, Anna Segal, Dash Longe, Forrest Jillson, Amie Engerbretson, Seth Wescott, Jim Ryan and others carve the faces of remote mountain ranges and pursue the thrills of a life lived in high places.
Stowe Jewish Film Festival - Sammy Davis, Jr: I've Gotta Be Me
Wednesday, July 24, @ 7:00pm
The Met Opera Encore in HD: La Bohème (Puccini)
Thursday, July 25, @ 2:00pm
Stowe Jewish Film Festival - Joseph Pulitzer: Voice of the People


text:
Q: R New dataframe with binary columns I am trying to create a new dataframe, that shows if a promo code is being used on a specific date(binary not the actual sum\count) but also sums the amount of sales that code produced.
DATA:
+---------------+------------+--------------+
| Order Date    | Promo Code | Sales Amount |
+---------------+------------+--------------+
| 10-29-20      |   today20  |   50         |
+---------------+------------+--------------+
| 10-29-20      |   vip20    |   50         |
+---------------+------------+--------------+
| 10-29-20      |   today20  |   50         |
+---------------+------------+--------------+
| 10-28-20      |   vip20    |   50         |
+---------------+------------+--------------+
| 10-28-20      |   vip20    |   50         |
+---------------+------------+--------------+
| 10-27-20      |   pc20     |   25         |
+---------------+------------+--------------+
| 10-28-20      |            |   50         |
+---------------+------------+--------------+
| 10-28-20      |   vip20    |   50         |
+---------------+------------+--------------+
| 10-27-20      |            |   25         |
+---------------+------------+--------------+
| ....          |      ....  |   ....       |
+---------------+------------+--------------+
| ....          |      ....  |   ....       |
+---------------+------------+--------------+

NEW DATAFRAME
+---------------+------------+--------------+--------------+--------------+
|Order Date     | today20    | vip20        |  pc20        | Sales Total  |
+---------------+------------+--------------+--------------+--------------+
| 10-29-20      |   1        |   1          |    0         |  150.00      |
+---------------+------------+--------------+--------------+--------------+
| 10-28-20      |   0        |   1          |    0         |  100.00      |
+---------------+------------+--------------+--------------+--------------+
| 10-27-20      |   0        |   0          |    1         |   25.00      |
+---------------+------------+--------------+--------------+--------------+
| ....          |      ....  |   ....       | ....         |   ....       |
+---------------+------------+--------------+--------------+--------------+
| ....          |      ....  |   ....       | ....         |   ....       |
+---------------+------------+--------------+--------------+--------------+

A: Use dcast
I prefer to use data.table, so
library(data.table)

dt=setDT(yourdf)

#This will give you the column names but with the sum in each column.
dt.wide=dcast(data=dt, formula = Order_date ~ Promo_code, fun.aggregate='sum', value.var='Sales_amount')

#Adds sales_total column
dt$Sales_total = apply(dt[,-c('Order_date'),with=F],1,sum)

#Converts promo_code columns to binary
dt[,lapply(.SD,function(x) x[x>0]=1),.SDcols=unique(yourdf$Promo_code)]

redpajama_set_name: RedPajamaStackExchange


text:
Temple & Webster Group Ltd Company Snapshot
Temple & Webster Group Ltd operates within the Furniture stores sector. In addition to historical fundamental analyses, the complete report available to purchase compares Temple & Webster Group Ltd with three other furniture and home furnishing stores in Australasia: Adairs Ltd (2020 sales of 388.93 million Australian Dollars [US$299.64 million] of which 68% was Sale of goods and services - stores), Nick Scali Ltd (262.48 million Australian Dollars [US$202.22 million] of which 100% was Retailing of Furniture), and Joyce Corporation Limited (91.53 million Australian Dollars [US$70.52 million] of which 74% was Retail Kitchen showrooms).
Sales Analysis. During the year ended June of 2020, sales at Temple & Webster Group Ltd were A$176.57 million (US$136.03 million). This is an increase of 73.5% versus 2019, when the company's sales were A$101.76 million. This was the fifth consecutive year of sales increases at Temple & Webster Group Ltd (and since 2015, sales have increased a total of 576%).
Temple & Webster Group Ltd
1 Week 2.1% 13 Weeks 16.3%
4 Weeks -12.9% 52 Weeks 268.2%
Temple & Webster Group Ltd Key Data:
Ticker: TPW Country: Australia
Sub Industry: Furniture Stores
(Year Ending Jan 2021). Employees: N/A
Currency: Australian Dollars Market Cap: 1,353,135,006
Fiscal Yr Ends: June Shares Outstanding: 113,422,884


text:
Q: Issues getting a background image to appear I am unsure of why I cannot get a background-image to appear in the following snippet. The url is correct and I have set size to the image. Also, how can you align a background-image in the center of a page? I know there are properties like right top, but I do not see one for center vertically and horizontally.
Thanks.

$("#arrow-icon").slideToggle(1000);
.arrow {
  height: 400px;
  width: 100%;
  background-color: blue;
  text-align: center;
}
#arrow-icon {
    padding-top: 100px;
    display: none;
    background-image: url("http://optimumwebdesigns.com/icons/down-arrow.ico");
    background-repeat: no-repeat;
    height: 50px;
    width: 50px;
}

A: The problem is that the div is smaller that the picture.
You can get around this with the background-size property
Example:
#arrow-icon {
    padding-top: 100px;
    display: none;
    background-image: url("http://optimumwebdesigns.com/icons/down-arrow.ico");
    background-repeat: no-repeat;
    height: 50px;
    width: 50px;
    background-size:100% 100%;
}

fiddle - https://fiddle.jshell.net/800modgt/
Or you can change the div width and height to the image width and height...

A: And in terms of centering, simply use:
background-position: center;

That said, I'm noticing that it's not center on the page on the Fiddle previously posts. You can use
margin:auto;

to center a
 horizontally
You might consider for the positioning using CSS3 for positioning, as it's very versatile in changing position of a div and how far it slides out. Here is a JSFiddle. It's for side animation, but it will work for just a standard up/down, too.
https://jsbin.com/yovaqo/edit?html,css,js,output


Welcome to the wonderful world of not knowing what the hell's going on.
1. Reply to this post with two or more caps (500x275px), I would prefer if you show me some caps of Juliet and/or Kate.
2. Your name, age and your fandoms would be nice.
If you don't already know, I'm Jørgen.


text:
What Jesus Did! 'Peer Fear' — Matthew 14:1-5
When Herod Antipas, the ruler of Galilee, heard about Jesus, he said to his advisers, "This must be John the Baptist raised from the dead! That is why he can do such miracles."
For Herod had arrested and imprisoned John as a favor to his wife Herodias (the former wife of Herod's brother Philip). John had been telling Herod, "It is against God's law for you to marry her." Herod wanted to kill John, but he was afraid of a riot, because all the people believed John was a prophet.
— Matthew 14:1-5 NLT
Key Thought
We sometimes talk about peer pressure and its power over teenagers. But if we look at ourselves, I think we'll find that adults are at least as vulnerable to peer pressure as teenagers. Just look at how we fall into line with fashion clothing, cologne, cars, neighborhoods, political opinions, morals, and hairstyles. Even our leaders seem to check which way the wind is blowing and gather the latest poll data before they will announce their opinions on any given subject. Today's verses reveal Herod's fear and weakness. He is afraid John is risen. He was afraid of John because of his adulterous relationship with his brother's wife —an incestuous relationship according to the Jewish law — and John's willingness to speak the truth. He was afraid to kill John because of the people's fascination with John. (As Matthew 14:6-12 shows, he had John killed in spite of his fear.) Herod is not a statesman ruler; he's a vulnerable reactor to political winds. We, too, forfeit our right to be called people of integrity if we cave in to the pressure of those around us and abdicate our values, principles, and beliefs to avoid scrutiny. No matter what accomplishments we may have, they are worthless because they are never enough if we fear losing recognition for them, losing the support of others, or losing the notoriety of our achievement. As Jesus disciples, recognition that nothing can separate from God's love and that he gave his only Son to adopt us into his family (Romans 8:15-17, 38-39; John 3:16-17) should be more than enough to validate our value to God and dispel any tendency toward fear.
Today's Prayer
Holy God and Divine Father, forgive me for the times that I have backed down from my convictions because of peer pressure. Give me the Spirit of power and boldness to stand for you not only when it is easy, but also when it is difficult. Help me be a person of proven integrity and faithfulness. In Jesus' name, I pray. Amen.
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What Jesus Did! is a series of one-year devotional guides through each gospel, using one short scripture passage each day and following the Gospel in sequential order. Each devotional consists of a scripture passage and a reflection and a prayer which open up the day's scripture and show how it challenges you to live for Jesus.
What Jesus Did! is written by Phil Ware and is available in book form.
Scripture quotations are taken from the Holy Bible, New Living Translation, copyright ©1996, 2004, 2007, 2013, 2015 by Tyndale House Foundation. Used by permission of Tyndale House Publishers, Inc., Carol Stream, Illinois 60188. All rights reserved.
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