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Category: Guardian
Guardian omits vital facts and context in story on delayed Palestinian mail
Once again, the Guardian has denied readers the full story, omitting crucial details, failing to provide relevant context and erasing nuance – all of which helps advance the desired Guardian narrative, one which invariably imputes maximum Israeli malevolence to any dispute between the two parties.
Whilst it would be wrong to blindly accuse journalists and editors of being personally antisemitic, some within the top echelons of media group – though at times condemning antisemitism in the abstract – seem, much like Corbyn himself, to hold the values and concerns of the mainstream British Jewish community – united in the belief that Labour Party leader represents, as The JC phrased it, "an existential threat to Jewish life in the UK" – in utter contempt.
As long as they're held hostage to the theory that powerlessness grants a degree of impunity against universal moral standards, such an intuitive causal relationship between Hamas's actions and Gaza's economic and political outcomes will continue to allude Owens and his fellow travelers within the Corbyn-left.
UKMW prompts Guardian correction to claim the Balfour Declaration granted Palestine to the Zionist Federation
By Adam Levick on August 8, 2018 • ( 2 Comments )
The Guardian upheld our complaint to an article which erroneously claimed that the Balfour Declaration promised "the land of Palestine" to the "Zionist Federation" of the UK and Ireland.
Corbyn's comments on Shalit prisoner swap mirror the tortured logic of the Guardian's Deborah Orr
By Adam Levick on August 2, 2018 • ( 29 Comments )
The Guardian's Deborah Orr was widely mocked for her bizarre argument that the Shalit prisoner swap with Hamas demonstrated Israeli racism, and she was eventually forced to offer an 'apology' of sorts. Corbyn's 2012 comments on Press TV mirror Orr's, and provide another illustration of the truly warped thinking which animates many anti-Zionist activists in the UK.
Guardian op-ed by Daniel Barenboim distorts nation-state law and Israel's founding principles
By Adam Levick on July 26, 2018 • ( 14 Comments )
Daniel Barenboim, in his Guardian op-ed, follows in the Guardian tradition of expressing contempt for the state by claiming that its policies betray the country's founding principles, whilst distorting both the policies and founding principles. His piece attacking the Jewish nation-state law grossly mischaracterises both the bill and the founding Zionist ideals (articulated in Israel's Declaration of Independence) it is said to betray.
Following our criticism, Guardian journalist adds stats on injured Syrians treated by Israel
Following out tweet to a Guardian journalist, criticising his claim that Israel has only treated injured Syrians "on occasion", he amended the article to include statistics we provided on the thousands of Syrians treated since 2013.
Guardian obfuscates Israel's humanitarian aid to Syrians
The Guardian is not interested in articles that challenge their readers' prejudices against Israel, especially such a counter-intuitive story which offers a glimpse at how Israeli humanitarian efforts has the potential to win some hearts and minds hearts in the hostile Arab world.
Guardian revives debunked smear that Ethiopian Israelis were given contraception against their will
Whatever the merits of Landsman's arguments about antisemitism, the fact that he recycled such an insidious smear with no basis in fact is another good illustration of the rank ignorance which informs much of the anti-Israel bigotry shared by the leadership and activist base of the British Labour party.
Guardian cartoon of Abbas in an Israeli straitjacket illustrates the media's failure to hold Palestinians responsible.
By Adam Levick on July 4, 2018 • ( 6 Comments )
The failure of media outlets to recognize that Palestinians are more than just victims and, even within the real limits imposed by the occupation, have the capacity to resist violence, hatred, scapegoating and self-pity, and embark on a path of real political and cultural reform, continues to deny news consumers an accurate understanding of the Israeli-Palestinian conflict.
The Guardian channels its inner Jeremy Corbyn
By Adam Levick on July 2, 2018 • ( 30 Comments )
When, in May, the Board of Deputies' outgoing president Jonathan Arkush met with Jeremy Corbyn, he asked the Labour Party leader: "Why is there nothing good you can say about Israel? According to Arkush, Corbyn didn't respond, but remained silent – a silence likely driven by the same "mythical Israel" that continues to haunt the political imagination of Guardian editors.
Guardian fails to correct erroneous claim by J Street leader Jeremy Ben-Ami
By Adam Levick on June 28, 2018 • ( 3 Comments )
Jeremy Ben-Ami, the founder and president of J Street, the far-left and self-proclaimed 'pro-Israel' US lobbying group, penned an attack piece in the Guardian on the US Ambassador to Israel which includes some of the favorite tropes of the pro-Palestinian commentariat.
Guardian appears to reject IHRA Working Definition of Antisemitism
By Adam Levick on June 20, 2018 • ( 42 Comments )
Contrary to the views of the Guardian Readers' Editor, the ideological similarity between tropes concluding that "Zionists are our misfortune" and tropes concluding "Jews are our misfortune" is simply impossible to deny.
Guardian amplifies B'tselem propaganda on the "barbaric" Israeli policy of enforcing its own laws
In short, Zonshein's op-ed on the planned eviction of illegal Bedouin encampments employ all the tools within the Guardian's delegitimisation playbook: lies, half truths, the use of hyperbolic language and completely unsubstantiated accusations of criminality to characterize Israeli policy.
Tweets by British journalist Sarah Helm demonstrate extreme anti-Israel bias
By Adam Levick on June 6, 2018 • ( 15 Comments )
We surveyed hundreds of tweets, spanning three years, by British journalist Sarah Helm and uncovered what we suspected all along: Helm is a pro-Palestinian activist. Not a journalist.
UKMW prompts Guardian to replace misleading photo accompanying article on UK arms sales to Israel
By Adam Levick on May 29, 2018 • ( 8 Comments )
We tweeted the Guardian's head of photography, Fiona Shields, alerting her to the inappropriate photo accompanying an article on increased UK arms sales to Israel. Shields promptly replaced the photo with one more appropriate.
Guardian fails to correct false claim – their 'journalist' calls our complaint "nonsense"
COGAT, the Israeli authority tasked with processing such travel requests, flatly denied the Guardian claim that a young Palestinian suicide victim had been denied a travel permit to study at Hebrew University. In fact, they said he had never applied for permission in the first place.
Guardian contributor suggests that Israel supporters are morally sub human
By Adam Levick on May 22, 2018 • ( 116 Comments )
If you defend Israel's actions in Gaza, your ethical impulses are, according to the Guardian columnist, not those of an ordinary human being. It would be difficult to find a better illustration of why so many Jews believe that media coverage of Israel incites antisemitism than a column suggesting that they, by virtue of their pro-Israeli political views, are morally deranged, even sub-human.
Guardian falsely suggests that Hamas has "softened" its call for Israel's destruction
Contrary to the Guardian Jerusalem correspondent's claim, Hamas has not "softened" its founding charter's rejection of Israel's existence, nor has it abandoned its promotion of violence to achieve this end.
Guardian editorial on Gaza riots again illustrates its unbridled hatred towards Israel
By Adam Levick on May 15, 2018 • ( 11 Comments )
Guardian accuses Israel of "war crimes" in shooting "protesters" who "posed no credible threat".
However, in what world do thousands of rioters attempting the breach the border of a sovereign democratic state, at the behest of a proscribed terror group, not represent a "credible threat"?
‹ Newer 1 2 3 4 5 6 7 8 9 10 11 … 162 Older ›


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Q: Finder file icon badging (icon overlay) on Mac OS X >= 10.6 I'm searching for a solution to do File icon overlays (icon badging) (like Dropbox does it on mac) with cocoa on Mac.
Does anyone knows a way to do this?
I've searched the Xcode docs and looked into scpplugins source code which is kind of old carbon code.

A: A litte bit late, but maybe will be help someone.
I solved same problem with class NSWorkspace (see setIcon:forFile:options)
Basic idea:

*

*Try to get preview of file with QLThumbnailImageCreate (if not NULL you will get thumbnail icon)

*If you didn't get thumbnail, then get default OS X icon for file (NSWorkspace iconForFile)

*Combine thumbnail (or default icon) with your badge

*Set new icon to the file (NSWorkspace setIcon:forFile:options)

A: Since the Finder was reworked in Snow Leopard, the older Carbon methods will no longer work.  The route I've taken to be able to badge icons in Finder involves writing a custom bundle which then needs to be injected into the Finder.
Look into Wolf Rentzsch's mach_inject (https://github.com/rentzsch/mach_star/tree/master/mach_inject) to be able to inject a custom bundle to a Cocoa application.
Use class-dump to be able to get a look into the header files of a Cocoa application (such as the Finder in Snow Leopard and Lion) to get an idea of what you will need to override in your own bundle.

A: I know this is an old question.
In recent time there is a library that implement this functionality: https://github.com/liferay/liferay-nativity.

A: NSDockTile makes this very simple:
NSDockTile *dockTile = [NSApp dockTile];
[dockTile setBadgeLabel:@"33"];

A: You can use the following two methods to have icon overlay over the folders/files.

*

*You can use -setIcon:forFile:options: method on NSWorkspace if you want to change the icon of a file or folder in Mac OS X.
However after you apply icon overlay using this method, overlay exits even though you moved that file/folder outside. This may not be the exact solution.

*Instead use the Finder Sync Extension target (File - New - Target - Finder Sync Extension) inside your app.
Once you created the extension, your application doesn't have direct communication with this target. In order to activate, use AppleScript command (I don't think there is a better alternative for this.)
To activate
NSString *pluginPath = [[[NSBundle mainBundle] builtInPlugInsPath] stringByAppendingPathComponent:@"yourextension.appex"];

NSString *pluginkitString = [NSString stringWithFormat:@"pluginkit -e use -a \"%@\"", pluginPath];
system([pluginkitString cStringUsingEncoding:NSUTF8StringEncoding]);

Once target activated, there are couple of ways were our application can communicate with that extension. Few of them are:
Using NSDistributedNotificationCenter. This class provides a way to send notifications to objects in other tasks(like the extension here).
Other way is to use:
[[NSUserDefaults alloc] initWithSuiteName:@"teamid.com.company.test"];

Both your application and the target should have common group identifier(i.e "teamid.com.company.test").
For this enable "App Groups" under Target - Capabilities - App Groups and give the identifier like the above (i.e"teamid.com.company.test") were teamid is the id that you will get it from your apple developer portal. Do the same steps for your Extension target as well.
Before to conclude, be sure that extension is activated or not. To check that go to System Preference - Extensions - Your App Finder.
This is the global point were user can enable/disable icon overlay for your application.

redpajama_set_name: RedPajamaStackExchange


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I'm in a battle with the Winter Blues. In an attempt to win this nagging fight, I'm trying to think happy thoughts, smile like the sun is shining and keep my mind and body busy. Sadly, the Blues have had a chance to stomp on me a few times. Like Tom Cruise to a couch. Without the happy dance.
A friend of mine sent me a Daily Inspiration that said, "During the short days of winter, we see less of the sun, but it hasn't disappeared. The earth has moved, but the sun is still shining bright and clear." So I will also remember that the sun is always shining, even when I can't see it.
If you, too, are a victim of the Winter Blues, maybe these pictures will inspire you to add some happy colors to your winter world. If nothing else, perhaps it will just make you smile for a moment.
Posted in DIY Decor, LOL (humor) and tagged Colorful, colors, Depression, DIY, Do it yourself, Happy, happy colors, home decor, home decorating, Home Improvement, The Blues, Vibrant, Winter Blues. Bookmark the permalink.


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Q: how to parse this xml with LINQ My XML has multiple elements but when I try to parse them using LINQ, I just get one element. Something is wrong with my select statement, but I'm having a devil of a time understanding what is wrong. The Result should be a dictionary populated with the InvoiceUid and InvoiceNumber for all the (2) invoiceListItems
Here is my XML:
    <?xml version="1.0" encoding="utf-8"?>
    <invoiceListResponse>
      <invoiceList>
        <invoiceListItem>
          <invoiceUid>39165890</invoiceUid>
          <invoiceDate>2013-12-26</invoiceDate>
          <invoiceNumber>W10001888</invoiceNumber>
          <contactUid>8363070</contactUid>
        </invoiceListItem>
        <invoiceListItem>
          <invoiceUid>39149309</invoiceUid>
          <invoiceDate>2013-12-24</invoiceDate>
          <invoiceNumber>W100</invoiceNumber>
          <contactUid>8363070</contactUid>
        </invoiceListItem>
      </invoiceList>
    </invoiceListResponse>

and the meat of my code:
         Dim list As XmlDocument = proxy.Find(queries)

        Dim InvoiceList = XDocument.Parse(list.InnerXml)
        ' get all <InvoiceListItem> elements from the xdocumetn
        Dim InvoiceListItems = From invoiceListItem In InvoiceList...<invoiceList>
                                 Select invoiceListItem

        'go through each InvoiceListItem in InvoiceListItems
        For Each InvoiceListItem In InvoiceListItems
            Console.WriteLine("Uid is {0} and Invoice Number is {1}", InvoiceListItem...<invoiceUid>.Value, InvoiceListItem...<invoiceNumber>.Value)
            returnInvoiceList.Add(InvoiceListItem...<invoiceNumber>.Value, CType(InvoiceListItem...<invoiceUid>.Value, Integer))
        Next
        Return returnInvoiceList

A: The problem is that your select statement is selecting invoiceList elements (of which there is only one). This should do what you expected:
Dim InvoiceListItems = From invoiceListItem In InvoiceList...<invoiceListItem>
                       Select invoiceListItem

A: ah finally got it working by adding .Elements here:
For Each InvoiceListItem In InvoiceListItems.Elements


If you've been following our posts on the Month of the Novel Facebook page, you know that we've been loading the footage (a technical filmmaking term for our video and audio files) into our computers and getting it all labeled. You might also know that there are a total of 1,349 raw source files.
Somehow, all these files will be edited into ten episodes for your viewing pleasure. Right now, though, we're taking a short break before diving in.
Stay tuned. We might just have some little tidbits coming soon.
This entry was posted in Production Blog and tagged editing, footage by Jordan. Bookmark the permalink.


Meet Jennie! RASKC is extremely pleased to honor Jennie who performs outstanding work in several roles at RASKC. Many people have noted on Jennie's tremendous proficiency, very pleasant demeanor, and exceptional attendance. She has been working a weekly shift on our Cat Meet and Greet Team since 2015. She has been walking dogs at RASKC since 2016. Jennie has trained dozens of new volunteers in both roles. She also leads RASKC's monthly family outreach event at Seattle Meowtroplitan Cafe and has been invaluable to in our social media presence thanks to all her postings of all our adoptable pets on Instagram. Thank you, Jennie!
A: I volunteered at RASKC about 14 years ago I did a dog training class for people who had recently adopted. Then I stepped away until 4 years ago. A friend of mine did cat meet and greet with her daughter and i thought it would be something fun to do with my son.
A: I have always loved all animals and wanted to work with animals. I trained dogs for about 11 years until I moved here from Colorado. Volunteering at the shelter gives me the opportunity to work with animals and people who love animals too.
A: I am a preschool teacher. I love to work with children and watch them discover and learn new things.
A: I have made many friendships with people whom I would may have never known, that is fulfilling in itself, but to be able to put my skills to use to help the OCT's and ACT's as well as the vet staff and make their jobs easier is what i'm there for.
Q: What is your life motto? I guess I never thought of a life motto.
A: I believe in treating all things, even spiders, with kindness and respect.
Q: If you have a dog, what is your favorite park to take your dog for a walk? What was a favorite activity that you liked to do with your dog?
A: My dog is old now and unable to take walks but when he was younger we walked on the Soos Creek Trail. He loved to get his feet wet in the Cedar River too.
Q: If you have any cats, what's the best thing about your cat?
A: They both brought much needed youth to our home.
A: My cats are the best. Both were adopted here Ben who is 1 year old is the crazy inquisitive one who will not be detoured from what he wants. Anakin, our newest addition is sweet and kind and loves to ride on my shoulder.
A: I would say research, especially when adopting a dog. Don't just get that cute puppy, know the breed, know what it takes to own them and be committed to giving that pet the love and attention it deserves.
A: I have had many, I grew up on a non-working farm in the mountains of Colorado. We had all kind of animals and not a whole lot of things to do. I spent my time training all the animals. My biggest accomplishment was teaching one of our chickens, Roxy, to sit. Proof that any animal can learn.
A: If you have time to give, do it. Volunteering is so fulfilling and so worth it.
Next Post Happy Tails: Django gets adopted!


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Archives Animal Breeding Archiv Tierzucht
Editorial & advisory board
fbn-dummerstorf.de
AAB | Articles | Volume 61, issue 4
Arch. Anim. Breed., 61, 481–489, 2018
https://doi.org/10.5194/aab-61-481-2018
Original study 19 Dec 2018
Original study | 19 Dec 2018
Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia
Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy... Sihem Amiri et al.
Sihem Amiri1, Bayrem Jemmali2, Mohamed Amine Ferchichi1, Hajer Jeljeli2, Rekik Boulbaba2, and Abderrahmane Ben Gara2 Sihem Amiri et al. Sihem Amiri1, Bayrem Jemmali2, Mohamed Amine Ferchichi1, Hajer Jeljeli2, Rekik Boulbaba2, and Abderrahmane Ben Gara2
1National Agronomic Institute of Tunisia, 43 Charles Nicoles street 1082, Tunis-Mahrajène, Tunisia
2Laboratory of Improvement and Integrated Development of Animal Productivity and Food Resources, Higher School of Agriculture of Mateur, University of Carthage, Tunis, Tunisia
Correspondence: Sihem Amiri (sihem.ouerghi@yahoo.fr)
Received: 07 Aug 2018 – Revised: 25 Oct 2018 – Accepted: 29 Nov 2018 – Published: 19 Dec 2018
Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI(+//−) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and (−/−)//(+/−)//(+/+) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI(+/−) cows tended to have a longer CI and DI than MspI(+/+) and MspI(−/−) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program.
Amiri, S., Jemmali, B., Ferchichi, M. A., Jeljeli, H., Boulbaba, R., and Ben Gara, A.: Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia, Arch. Anim. Breed., 61, 481–489, https://doi.org/10.5194/aab-61-481-2018, 2018.
The availability of dense genomic information has increased genome-wide association research for quantitative traits in bovine species. Fertility is considered to be a functional polygenic trait controlled by numerous genetic loci and biased by environmental factors. Hence, linked candidate genes of the encoding loci are searched to optimize reproductive performance.
Growth hormone (GH) belongs to a family of somatolactogenic hormones which include placental lactogen, prolactin and anthers hematopoietic growth factors (Cosman et al., 1990). GH is an anabolic hormone synthesized and secreted by the somatotroph cells, it is a major regulator of postnatal growth and metabolism in mammals, and it plays an important role in lactation, protein, lipid and carbohydrate metabolism, tissue growth, and fertility in cows (Akers, 2006; Lucy, 2008; Thidar et al., 2008). The growth promoting and metabolic actions of GH are generally mediated by the insulin-like growth factor I (IGF-I) (Ramesha et al., 2015).
GH is a polypeptide hormone with a sequence 191 amino acid, its length is approximately 1800 bp and its production is linked to chromosome region 19q26 in the bovine genome (Hediger et al., 1990) with five exons and four introns.
Several polymorphisms of the bovine GH gene have been described and three polymorphic sites have been identified within the fifth exon of this gene (Yao et al., 1996)
Many researchers have studied the associations of GH gene polymorphisms with production traits in dairy or beef cattle (Balogh et al., 2009a; Heidari et al., 2012) owing to the critical role of GH with respect to growth, body composition, metabolism regulation, lactation and mammary gland development (Lagziel and Soller, 1999). A significant relationship between polymorphisms in the bovine GH gene and lactation performance has been reported (Zhou et al., 2005; Balogh et al., 2009b; Mullen et al., 2010).
Lucy et al. (1993) reported a polymorphic site for AluI restriction endonuclease, due to cytosine to guanine transversion at position 2141 which induced a change of the leucine (L) into a valine (V) in the amino acid sequence at position 127 of the protein chain of bovine GH. The genotype of GH AluI polymorphism was reported to be associated with birth weight in dairy cattle (Biswas et al., 2003) and mature live weight in beef cattle (Zwierzchowski et al., 2001). Current knowledge regarding the interrelation between AluI polymorphism and reproduction is limited, and only a few studies have investigated this association.
No significant association has been reported between AluI polymorphism and the following traits: age at the first calving (Kovács et al., 2006; Grossi et al., 2015), the number of oocytes suitable for in vitro maturation, the number of matured oocytes, the mean oocyte diameter, and the number of embryos produced (Lechniak et al., 2002). However, a significant association was observed for milk yield, milk fat and protein (Dybus, 2002; Kovács et al., 2006), in addition to beef bulls non-return rates (Lechniak et al., 2002).
The GH gene was digested with MspI restriction endonuclease; GH-MspI polymorphism is located in intron 3 of the GH gene at position 1547 (Zhang et al., 1993). As a consequence, two alleles occur, and MspI (−) contains a T insertion at the +837 position and a C–G transition at the +837 position (Lee et al., 1994).
To date, several studies have investigated the association of GH-MspI polymorphism with milk and meat production traits (Zhou et al., 2005; Beauchemin et al., 2006; Katoh et al., 2008), but only a few studies have examined its effect on reproductive traits (Unanian et al., 2002; Lechniak et al., 2002; Gorbani et al., 2009).
Based on the abovementioned evidence, the present investigation was carried out to identify GH-AluI and GH-MspI polymorphisms and uncover their association with reproductive traits in Holsteins dairy cattle.
2 Materials and methods
2.1 Experimental design
Four hundred and ten (410) Holstein dairy cows from the Agricultural Development farm in northern Tunisia were included in the present work. These animals were either imported as pregnant heifers from European countries or were born on the farm.
Cows were observed for signs of estrus twice daily, as well as during the morning and afternoon milking. Animals detected in estrus were artificially inseminated. Pregnancy diagnostic by rectal palpation was performed during the 60–70 days after insemination, and pedigree information was available for all cows in the herd.
All cow milk production and reproductive performance data were systematically recorded spanning the time period from January 2013 to December 2017. The reproduction data were derived from the national Office of Livestock and Pasture (OEP) database.
2.2 Blood samples
Five milliliters of venous blood was collected under sterile conditions, from the jugular vein of animal using a 5 mL polypropylene centrifuge tube containing 0.5 mL of 2.7 % EDTA solution as an anticoagulant. The experiment was carried out with the approval of the Institutional Animal Ethics Committee (IAEC). The centrifuge tube was tightly capped and shaken gently to facilitate the thorough mixing of the blood with the anticoagulant. Blood samples were transported to the laboratory in an icebox containing ice packs and stored in a refrigerator at −20 ∘C until the isolation of the DNA was carried out.
2.3 Genotyping
Genomic DNA was isolated from frozen blood using the NucleoSpin Blood kit (innuPREP Blood DNA Mini Kit) following the manufacturer's instructions. The integrity of the DNA was examined using electrophoresis on a 1 % agarose gel, and acceptable samples were used for PCR reaction.
Genotyping was performed using a PCR reaction mixture containing 4 µL of genomic template, 2.5 µL of buffer, 2.5 µL of dNTP, 2.5 µL of each of the primers (F/R), 0.2 µL of the heat-resistant DNA polymerase (Taq polymerase) and 10.8 µL of autoclaved distilled water in a final volume of 25 µL. The primers used were designed to amplify 428 and 329 bp GH gene fragments. Table 1 shows the primers used for amplification.
PCR was carried out with an initial step of 95 ∘C for 5 min, followed by 35 cycles of 95 ∘C for 30 s, 59 ∘C or 60 ∘C for 30 s (for AluI and MspI, respectively), 72 ∘C for 30 s and a final extension of 72 ∘C for 10 min. The PCR amplification yielded 428 and 329 bp fragments that were revealed using electrophoresis on a 2 % agarose gel.
Restriction endonuclease AluI and MspI were used to digest a 428 and 329 bp PCR product, respectively. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) reaction requires 12 µL of the PCR product, 0.2 µL of BSA (bovine albumin serum), 2.5 µL of the reaction buffer, 0.3 µL of the restriction enzyme, 10 µL of autoclaved distilled water in a total volume of 25 µL incubated at 40 ∘C for at least 11 h. The PCR-RFLP fragments were separated using horizontal electrophoresis in 2.5 % agarose gels stained with ethidium bromide and visualized under a UV transilluminator, the gel was documented using Gel Doc equipment, and the genotypes were determined according to band sizes.
Allele and genotype frequencies were calculated using genotyping data from each gene based on the population analyzed. Chi-square statistics (χ2) were used to check whether the populations were consistent with the Hardy–Weinberg equilibrium.
Table 1Primer sequences, sizes of the amplified fragments in PCR and sizes of the observed genotypes.
2.4 Reproductive traits
Lactation and several other reproduction traits were used to investigate the effect of the polymorphisms studied on the performance of dairy cows. All data regarding cow milk production and reproductive performance were systematically recorded spanning the time period from January 2013 to December 2017; the following traits were derived from these records: the integrated age at first calving (AGE_1CALV), the calving interval (CI), the number of inseminations per conception (NINS) and the days open (DO; the interval between calving and the next successful insemination). These traits are believed to be the most important and available parameters of reproductive efficiency for the herd. Descriptive statistics for these traits are presented in Table 2.
Table 2Descriptive statistics for reproductive parameters.
The statistical analysis was performed by repeated measures using the MIXED procedure in SAS software (SAS 9.0 for Windows). The resulting mixed linear model included the following effect which was used to evaluate the impact of GH polymorphisms on reproduction traits of cows:
yijklmn=μ+GAlui+GMspj+origink+seasonl+paritym+lamenessn+eijklmn,
where y is the variable dependent on animal, μ is the overall mean, GAlui is the fixed effect of the GH-AluI genotype (LL, LV, VV), GMspj is the fixed effect of the GH-MspI genotype (−/−, +/−, +/+), origink is the fixed effect of the cows' country of origin (k=3), seasonl is the fixed effect of the of calving season (l can represent a value from 1 to 4 which refers to winter: December–February, spring: March–May, summer: June–August, autumn: September–November, respectively), paritym is the fixed effect of the order of parity (m=4), lamenessn is a fixed effect that indicates whether the cow has been diagnosed with lameness or not (n=2) and eijklmn is the random residual error.
The F statistic and the T statistic were used to compare the effect of each factor. A probability of p≤0.05 and p≤0.01 were considered to be statistically significant and highly significant, respectively.
3.1 Gene frequency
The DNA isolated was of a good quality. The 428 and 329 pb fragments from the GH gene were characterized and successfully amplified from the DNA of each sample; this indicated a strong conservation of the DNA sequence existing in cattle.
The χ2 test showed that the allele frequency and genotypes of GH-AluI and GH-MspI were in Hardy–Weinberg equilibrium.
Visualization under UV light revealed that GH-AluI exhibited three band patterns. Fragments that represent the leucine variant (LL) gave rise to the 265//96//51 pb band, AluI fragments gave rise to the 265//147 pb band that represented the valine variant (VV), and the (LV) pattern gave rise to the 265//147//96//51 pb band.
The following DNA restriction fragments were also obtained as a result of MspI restriction: 329 bp for the MspI(−/−) genotype, 329//224//105 bp for the MspI(+/−) genotype and 224//105 bp for the MspI(+/+) genotype.
Table 3 shows genotypic and allelic frequencies of GH-AluI and GH-MspI polymorphisms in the herd studied. GH-AluI genotype frequencies were 77.75, 18.59 and 3.66 for LL, LV and VV, respectively. These results showed that the L allele was significantly more frequent than the V allele (87.045 vs. 12.955).
The GH-MspI frequency distribution showed that the MspI (+/+) genotype was the most abundantly found (55.50), followed by MspI (+/−) (29.13). The least frequently found genotype was MspI (−/−) (15.37). The MspI (+) allele was more frequent than the MspI (−) allele (70.065 vs. 29.935).
Table 3Genotypic and allelic frequencies (%) in the GH-AluI and GH-MspI polymorphisms.
3.2 Factors affecting reproduction traits
The investigation of the effect of non-genetic factors on reproduction traits showed that parity only had a significant effect on the NINS (p<0.0001) but had no effect on the CI or the DO. The cows' country of origin significantly affected the NINS (p=0.0003) and the AGE_1CALV (p=0.0009) but had no effect on the CI or the DO. The diagnosis of lameness had a highly significant effect on the CI (p=0.0009) and the DO (p=0.0025), a significant effect on the AGE_1CALV (p=0.0211) and no effect on the NINS. The calving season had no impact on any of the traits analyzed.
As shown in Table 4, genetic polymorphisms were found to be related to the abovementioned traits. The GH-AluI significantly affected the NINS (p<0.0001), the CI (p=0.0005) and the DO (p=0.0007) but did not affect the AGE_1CALV. However, the GH-MspI had no effect on any of the traits analyzed.
Table 4The effect of sources of variation on reproductive traits. DDL refers to degrees of freedom, and Pr refers to probability.
The effect of genetic polymorphisms on reproductive traits was also compared to the GH-AluI and GH-MspI variants (Table 5). The replacement of the L allele by the V allele was found to lead to an increase in the NINS, the CI and the DO, and the performance of the LL genotype was significantly higher compared with similar values of the VV and LV genotypes. Cows with the LL genotype had the lowest NINS (−0.5892) compared with cows with VV and LV genotypes – the difference was highly significant (p<0.0001).
The GH-AluI polymorphism had a substantial effect on the CI (p<0.0001), and the LL genotype shows a shorter CI (−80.76 days) compared with the VV genotype. The difference between the LV and VV genotypes was also considerable (p=0.0021) with a shorter CI for the LV genotype (−69.68 days). The statistical model that estimates the genotype effect revealed that the LL and LV genotypes were significantly associated with the DO (p=0.0002 and p=0.0030, respectively), and that LL and LV cows tend to conceive 78.6 and 66.87 days before VV cows, respectively. There was no effect of the substitution of the L allele by the V allele on the AGE_1CALV: VV cows tended to have their first calving 15.22 and 27.10 days later than LV and LL cows, respectively, but the differences were not statistically significant.
There was no detectable significant effect of the GH-MspI on the traits analyzed, although polymorphism had a suggestive effect on the CI (p=0.0926) and the DO (p=0.0714). MspI (+/−) cows tended to have the longest CI and DO, but the difference was still not significant.
Table 5Association of GH-AluI and GH-MspI genotypes with reproduction trait. DDL refers to degrees of freedom, and Pr refers to probability.
A statistical evaluation of the association of the GH-AluI–MspI combined genotypes on reproductive traits showed a significant effect on the NINS (p<0.0001), the CI (p=0.0024) and the DO (p=0.0022), although no effect was found on the AGE_1CALV (Table 6).
The estimate of the VV/− genotype intercept for the NINS, the CI, the DO and the AGE_1CALV is 2.610, 498.45, 218.79 and 812.61, respectively. However, differences in the combined genotype performance is only significant for the CI and the DO.
Table 6The effect of combined genotypes on reproductive traits in dairy cows.
There were no genetic factors (namely parity, origin, calving season and lameness) that clearly accounted for the reproduction traits examined in this study.
Parity showed a significant relationship with the NINS, with primiparous cows requiring a lower number of inseminations per conception than pluriparous cows. The impact of the cow's origin with respect to the NINS and the AGE_1CALV provided evidence that exported cows had better reproductive performance than native cows in this study. Regarding the antagonist correlation between lameness and reproduction traits, cows that were diagnosed with lameness did not perform as well as cows that were not diagnosed. In addition, differences in the feeding and climatic features of the calving season had no effect on the traits analyzed except for the fact that the NINS was slightly influenced by calving season.
The majority of cows were homozygous for the leucine allele (L; 77.75 %); only 3.66 were homozygous for the valine allele (V), whilst 18.59 % were heterozygous. Therefore the frequency of the V allele was lower than the L allele (87.045 vs. 12.955). Allele and genotype frequencies for the GH-AluI polymorphisms given in this work were in agreement with those reported in previous studies, which have been discussed extensively in Holstein dairy cows (Balogh et al., 2009b; Ruprechte et al., 2011; Grossi et al., 2015).
However, other findings have shown the dominance of the heterozygous genotype LV and the absence of the homozygous genotype VV (Aruna et al., 2004; Hadi et al., 2015).
The most frequent genotypes for GH-MspI were homozygous MspI(+/+) (55.50), whereas the homozygous MspI(−/−) genotype was the least frequent (15.37). Hence, the dominance of the Msp(+) allele was evident compared with the MspI(−) allele (70 065 vs. 29 935). Results of the present work are in agreement with those published by Dybus et al. (2002) in Polish Black-and-White cattle and by Zhou et al. (2006) in China Holstein cows. However, Hartatik et al. (2018) reported a higher frequency of the MspI(+/−) genotype than the MspI(−/−) and MspI(+/+) genotypes in Pesisir cattle and cross breeds, in addition to the fact that MspI(−) allele was more frequent than the MspI(+) allele (0.533 vs. 0.467).
There are only a few studies that have investigated the relationship between GH-AluI and GH-MspI polymorphisms and reproductive traits in Holstein dairy cattle. Evaluations of this relationship in this work showed a significant effect of the GH-AluI on the NINS, the CI and the DO but no effect on the AGE_1CALV. Furthermore, no significant association between the GH genotype and the interval from calving to first ovulation was observed by Balogh et al. (2009b).
Similarly, no obvious association of GH with AGE_1CALV and CI was observed in Canchim and Hungarian Holstein–Friesian cattle (Kovacs et al., 2006; Grossi et al., 2015) and with AGE_1CALV in a Hungarian Holstein–Friesian bull dam population (Katalin et al., 2006). Moreover, Kovacs et al. (2006) reported no noteworthy effect of GH-AluI on the CI, the number of calves or the dry period in a Hungarian Holstein–Friesian bull dam. Ruprechter et al. (2011) found no effect of the GH genotype on the calving–first service interval, the number of services per conception or the total pregnancy rate, and no other significant effects were observed with respect to the number of matured oocytes or the number of embryos produced (Lechniak et al., 2002).
In our study, the LL genotype is considered the most favorable with respect to reproductive traits, as cows carrying the LL genotype showed better performance than LV and VV cows – this difference was highly significant for NINS, the CI and the DO. Lechniak et al. (1999) reported no significant genotype effect for lower ejaculate volumes for leucine homozygous bulls and greater non-return rates of VV beef bulls at 60 days postpartum.
Sabour et al. (l997) reported that V/V genotypes were related to better milk traits, especially higher milk protein compared with the other genotypes. These findings are in line with our result if we consider the established antagonistic correlation between reproduction and the milk production function, which means that the GH genotype may directly or indirectly affect reproduction performance. In addition, numerous previous studies have suggested an additive effect of the L allele on milk production and contents with LL cows reportedly yielding more milk, fat and protein than LV and VV cows (Lucy et al., 1991; Shariflou et al., 2000; Dybus, 2002).
This examination of the impact of the GH locus on reproductive traits showed that GH-MspI polymorphism only had a suggestive, non-significant effect on the CI and the DO. To date, few studies have investigated the effect of GH-MspI polymorphism on reproductive traits of bulls, and the results of these studies have reported a significant association between GH-MspI polymorphisms and the following sperm quality traits in bulls: fresh sperm motility, sperm concentration, minor and major defects, scrotal circumference and testicular growth after puberty (Lechniak et al., 2002; Unanian et al., 2002; Gorbani et al., 2009); the reproductive traits of beef cattle have also been studied (Anggraeni et al., 2017).
Arango et al. (2014) reported that MspI polymorphism is associated with age at first service, first birth, first postpartum service and second birth. Furthermore, the MspI (+/+) genotype had the greatest effect on the age at first service and was considered the optimal genotype for reducing age at first service, first birth, first postpartum service and second birth. In addition, several inconsistent results have reported the effect of GH-MspI polymorphism on growth and milk production traits (Dybus et al., 2004; Pereira et al., 2005; Zhou et al., 2006; Katoh et al., 2008). MspI(+/+) cows are considered to yielded more milk, fat and protein than +/− individuals (Zhou et al., 2006).
Significant differences between cows with different GH-AluI–MspI combined genotypes were found at the 5 % level for the CI; cows carrying the LL/− genotype showed significantly (p=0.021) better performance, and their CI was 95.754 days lower than VV/− cows. The GH-MspI–AluI combined genotypes also significantly affected the NINS (P<0.0001); cows with the VV/+- genotype needed the highest NINS in the herd, although this tendency was not significant.
The effect of the combined genotypes was also notable with respect to the DO (p=0.0022), further confirming the difference between cows of different genotypes. The LL/− cows were significantly (p=0.0298) associated with the shortest DO compared to the estimated intercept of the VV/− genotype. However, no significant association was found between cows with different GH-AluI–MspI combined genotypes and the AGE_1CALV.
As previously stated, the Alu/LL genotype showed the best relationship with reproductive traits, whereas the GH-MspI only had a suggestive effect on the CI and the DO. Hence, the LL/− combined genotype can be expected to be associated with better performance. Very few studies have investigated the effect of GH-AluI–MspI combined genotypes in dairy cattle. However Dybuset al. (2004) reported that VV/++ cows produced milk with the highest fat and protein content.
5 Conclusions
In this study, AluI and MspI polymorphisms of the bovine GH gene were identified in a population of Holstein dairy cows in Tunisia which displayed L allele and MspI(+) allele dominance. GH-AluI significantly affected the NINS, the CI and the DO; however GH-MspI had no effect on any of the traits evaluated except for a slight tendency on the CI and the DO. GH-MspI–AluI combined genotypes had a highly significant affect on the NINS, the CI and the DO, and cows with the LL/− genotype tended to have better reproductive traits.
Under field conditions, the detection of polymorphisms in genes related to reproductive traits and the identification of the allele which results in a phenotype of interest can be considered as candidate markers of reproductive traits for cattle breeders.
The data are not publicly accessible; they are the property of the National Livestock and Pasturing Office (OEP) of Tunisia.
The National Livestock and Pasturing Office (OEP) are gratefully acknowledged for their contribution to this work and for making their database available. The authors are also grateful to the Chergui Society of Agricultural Development and their personnel for their kind and helpful contribution to this work.
Edited by: Steffen Maak
Reviewed by: Ghada Nouairia
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Genetic polymorphims in the growth hormone (GH) gene were studied in 410 Holstein dairy cows. Genotyping was carried out using PCR-RFLP. Data were analyzed by the MIXED procedure to reveal the effect of GH genotypes on reproductive traits. GH-AluI had a favorable effect on exanimate traits except for the age at the first calving. The homozygous LL genotype seemed to be advantageous for calving interval and days open. A significant effect of different GH-AluI–MspI combined genotypes was found.
Genetic polymorphims in the growth hormone (GH) gene were studied in 410 Holstein dairy cows....
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Q: System.MissingMethodException: Method not found: '?' when trying to build a custom dynamic type with a delegate method I am trying to dynamically build a type with a method that calls into an external delegate by using System.Reflection.Emit. However when I try to call this method, my program crashes with the exception in the title at the method call. Here's my code so far:
private static void TestMethodReal() => Console.Out.WriteLine("Inside TestMethod");

// In Main()
var method = typeof(Program).GetMethod(nameof(TestMethodReal), BindingFlags.Static | BindingFlags.NonPublic)!;

var builder = MyTypeBuilder.GetTypeBuilder("TestType");
var testMethod = builder.DefineMethod("TestMethod", MethodAttributes.Public, typeof(void), Type.EmptyTypes);

var generator = testMethod.GetILGenerator();
generator.EmitCall(OpCodes.Callvirt, method, null);
generator.Emit(OpCodes.Ret);

dynamic inst = Activator.CreateInstance(builder.CreateType()!)!;
inst.TestMethod(); // <--- Exception is thrown here

The MyTypeBuilder class and GetTypeBuilder method is from this answer, slightly modified to accept a parameter for the type's name.
This program is supposed to create a new dynamic class with a method called TestMethod that calls the actual TestMethodReal method, instantiate the class, and call the method.
What am I missing?

A: You're using the wrong dispatch mechanism!
OpCodes.Callvirt is for virtual method calls, eg. overridable instance methods, the resolution of which needs to be deferred until runtime.
For static method invocation you'll want a plain old OpCodes.Call instruction instead:
generator.EmitCall(OpCodes.Call, method, Types.EmptyTypes);


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Getty	Joel Embiid #21 of the Philadelphia 76ers looks on in the second half against the New York Knicks.
The Philadelphia 76ers will enter tonight's road tilt with the Oklahoma City Thunder without their starting 2 centers.
Joel Embiid will miss his fourth consecutive game due to tendinitis in his left knee, per Keith Pompey of Philly.com. Meanwhile, Boban Marjanovic remains out after a bone bruise and right knee sprain he suffered in Monday's 111-110 loss to the Pelicans.
The third-year center hurt his knee just before the All-Star break. While some questioned his participation for Team Giannis' 178-164 loss to Team Lebron, his coach Brett Brown deflected the criticism.
The team is expected to provide an update on Embiid before Saturday's game vs. Golden State. The original prognosis was a week, per Brown.
Embiid is sixth in the league in scoring at 27.3 points per game. He's tied for second in rebounds (13.5) and tied for seventh in blocks (1.94). He's raised his scoring output in all three of his NBA campaigns, going from 20.2 in his rookie year to 22.9 points last season.
On the other hand, he's had his fair share of boo boos since joining the league. Last February, he missed 17 of the last 18 games with a bone bruise and torn meniscus in his knee.
His foot has been an issue, as well. John Smallwood of Philly.com expressed his concern over his long-term health last winter.
Flash forward to today, and the likely explanation for Embiid's absence is load management. Embiid has played in 54 of the teams 58 games thus far in 2018-19, so Brown might just sit him until it's absolutely necessary.
Philadelphia is 2-1 since Embiid's absence, which includes two squeakers over the Heat and Pelicans and a 15-point loss at home to the Trailblazers.
Despite the winning record, the Philadelphia defense is missing Embiid's (and Marjanovic's) presence on the inside. The defense has ceded a little over 114 a night during this stretch. This would rank near the bottom of the league in scoring defense extrapolated over a season.
Noah Levick of NBC Sports Philadelphia provided a good video analysis of the defense sans Embiid.
In this @UnivestCorp film review, @NoahLevick examines the defensive issues exacerbated by Joel Embiid's absence, but also one factor that has contributed to the Sixers 2-1 record since that injury.
Levick cites a drop in "pick-and-roll defense," "slow rotations" and a "lack of a rim protector" for the defensive woes. Embiid and Marjanovic combine for nearly 3 blocks per night, so both absences have forced Brown to go to smaller lineups.
Former Serbian league center Jonah Bolden will carry the baton until both recover. He tallied 9 points and 6 rebounds in 18 minutes in the win over the Pelicans.
Joel Embiid will miss his fourth straight game due to his right knee sprain tonight as the Philadelphia 76ers face the Thunder in Oklahoma City.


text:
JAN KÖHLER
Interactive Media Design and Cinematography
JUSTICE AND PEACE NETHERLANDS
A video I produced for Justice and Peace Netherlands, from concept to filming to editing.
Jan's job at Sofar in The Netherlands is to film and edit video for each performing band, which encompasses the location, the performances and the spirit of Sofar Sounds, which is then uploaded to their youtube channel which now has over 900,000 subscribers.
Sofar Sounds is a community of thousands of artists, hosts, fans, travelers and more, putting on hundreds of secret, intimate events per month, across 438 cities around the world. A Sofar gig is an intimate show held in an unique venue, like someone's living room or an antique shop.
CORNERSTONE DOCUMENTARY
Jan worked on this documentary as a director of photography and won the African Film Festival Award for best cinematography.
The film, by award-winning director Debi Lang, touches on Twesigye Jackson Kaguri's moving personal story, which inspired the creation of Nyaka AIDS Orphans Project, a motivated, comprehensive, community-based organization that utilizes a unique holistic approach to not only combat hunger, illness and poverty, but also to educate children, create financial independence, and empower girls and women.
LUCITOPIA RURAL DESIGN CHALLENGE
Jan was invited to take part in the 6 day Lucitopia Rural Design Challenge which consisted of 10 teams from 7 international academies and design institutions. winning the third place award
Together with his team, Jan won the third place award by developing a concept for an App to promote ecotourism in the area. For the final presentation Jan also filmed, edited and did sound design for the promotional video in less than 24 hours.
CLUB NYX
My work with Club NYX in Amsterdam varies from promotional video to anniversary celebration films and edits.
Club NYX strives to resemble her one true love: the city of Amsterdam. It's a magical place that offers room to people of all walks and talks.
Design / Film
Jan Köhler is half German and half Mexican. He grew up in Honduras and moved to Europe in 2012. He is an interdisciplinary designer and film maker who graduated with a Bachelor in Interactive Media Design at The Royal Academy Of Arts in The Netherlands. His work ranges from documentary film making to concept development.
Jan shows most of his work through a combination of film, design, installations and interactive structures. He often works towards creating pieces, which can be integrated into society and serve a purpose, whether to question, or bring about change.
Thanks for contacting me!


Full, rich and warm sound, transparent with plenty of dynamics and punch. CRYOGENICALLY TREATED for the utmost in performance, low noise and long life. Low noise and microphonics selected for LINE LEVEL AND IF YOU RUN A SEPARATE HEAD GUITAR AMP.


Textile machines and their components are subject to high stress. Constantly changing high loads, vibrations and varying operating conditions require tough equipment. External factors such as moisture and extreme temperature variations come on top of that. To minimise wear, reduce costly downtime and extend maintenance intervals, speciality lubricants play a vital role. Klüber Lubrication presents its latest product developments and customised services at the ITMA 2015.


I need to know the same thing... I have an F22c with 164000 miles on it and I'm wanting to boost it. The power is outdated compared to other vehicles that are on the market. I just want it updated so that it will last another 200000 thousand miles but with boost.
I wouldn't touch the engine unless there's a reason to. Do a compression test and leak down to verify its health and if it checks out ok, you're good to go.
What is needed for 2.4 stroker engine?
rebuild engine that has 130k+ miles?
If you "rebuild" an abused engine, is it good as new?


text:
K Line begins project cargo shipments from Japan to UK
By Marcus Williams2015-09-08T15:56:56+01:00
Global shipping line, K Line, has begun a direct service between Japan and the UK, which will employ the company's 7,500 unit ro-ro vessels to move project cargo volumes (namely rail equipment) between the port of Tokuyama-Kudamatsu and Teesport.
K Line signed a contract in May this year with Vantec HTS Forwarding to transport Hitachi rail cars for the UK's Intercity Express Programme (IEP), which seeks to replace the existing InterCity 125 train fleet being on routes in the country. The new trains are being manufactured by Hitachi at its Kasado plant in Japan.
A spokesperson for K Line told Automotive Logistics that a total of 790 rail cars for the IEP will be transported over the next few years and the majority will be booked with K Line ro-ro vessels.
"Depending on booking requirement by the shipper, 5 to 18 rail cars will be loaded per vessel on almost monthly basis," said the spokesperson.
According to PD Ports, which owns Teesport, the K Line vessels will be the largest of their kind to arrive at the port, at a length of just under 200m and more than 75,216 gwt. K Line has ordered ten such vessels, which it said would give the company increased car carrying capacity of 20% as well as much more space for high and heavy cargo, including construction machinery and the project cargo being brought to Teesport.
The company also said the route between Japan and the UK would allow exporters of project cargoes to connect directly from Teesport with K Line's global network.
"We are pleased to announce this new service to Teesport," said a spokesperson for K Line. "The port's excellent infrastructure and its proximity to important North East markets makes it the perfect choice for our business and we are very much looking forward to working with PD Ports on this service."
K Line's announcement follows a similar contract in January this year when Höegh Autoliners vessel – Höegh London – delivered a rail carriage from Kobe in Japan to the port of Tyne in the UK for onward delivery to the Hitachi Rail Europe plant in County Durham, UK.
Ports/terminals


It's National Vanilla Pudding Day! To celebrate, we took a vanilla pudding pack and baked it into a moist Bundt cake topped with a rich blackberry glaze and fresh blackberries. This is an easy, but beautiful dessert perfect for your Memorial Day get-together.
Preheat oven to 350 degrees. Grease Bundt pan. In large bowl, combine pudding mix, cake mix, sour cream, eggs, water, and oil. Beat on medium speed for two minutes. Fold in vanilla chips. Pour mixture into Bundt pan. Bake 30-35 minutes or according to time indicated on cake box. Remove from oven and let cool for at least 20 minutes.
While cake is cooling, heat blackberry preserves over medium heat in a small saucepan; stir until melted. Once melted into a sauce-like texture, remove from heat. Add powdered sugar and stir together.
Once cake is cool, turn onto plate and drizzle blackberry glaze on top. Fill the middle and top of cake with fresh blackberries.


From The Pogues' fairytale of New York to carols such as In The Bleak Midwinter, Kerry McKittrick asks local personalities what's their favourite festive music to hear.
My all-time Christmas song is Fairytale of New York by The Pogues and Kirsty McColl. I heard the song in New York the October before it came out, as I had been sent over to interview the band just after they made the video. I was transfixed by it and played it again and again.
I was in New York for four days following The Pogues, as they were playing two shows at the Ritz. I finally got an interview with Shane MacGowan at a Japanese restaurant and he drank about 20 bottles of sake while we were there. When the bill was handed to the guy from the record company he told Shane he had just drank away the royalties of the whole single - no-one was expecting it to turn out the way it did.
It was a beautiful time and I encountered Kirsty McCall on a couple of occasions too. Every time I hear the song it puts a wee smile on my face."
My favourite song is Wizzard's I Wish It Could Be Christmas Every Day. It's the last song in our Christmas show right now, but that's not why I like it so much.
My mum and dad are singers and we would have gone to watch them in the social club at Christmas every year.
When they sang that song everyone knew exactly what it was as soon as the opening chords were heard and that was it - everyone was up on the dancefloor."
My favourite is actually a hymn - In The Bleak Midwinter. And as a massive James Taylor fan I think his version is the best I've heard. The song itself reminds me of when I was young and Christmas seemed more special than it is now. While I wouldn't call myself a religious person I do have faith, and back then it was much less about Santa and Christmas presents and more about the fact that we were celebrating the birth of Jesus. At the end of the day it is a Christian festival. The lyrics of the hymn are just beautiful - I believe they come from a Christina Rosetti poem - and the music is gorgeous. It reminds me of a time when we'd be going to lots of carol services and there was much less commercialism than there is today. When I was growing up we thought about what Christmas was about and I think we've lost that. I remember getting two or three gifts as a child - nothing too extravagant. Now, so many people put themselves into debt every year."
Have Yourself a Merry Little Christmas by Judy Garland is easily my favourite Christmas song - I've always loved it.
The song takes me right back to when I was at a kid and we would go to the Christmas service at midnight in church.
I remember one year being tucked up in blankets in the back of the car with my sister - my mum had an old 2CV which was always freezing.
We were looking out the window and we saw a shooting star. My sister told me it was either a shooting star or Santa on his way and I was very excited about that.
Weirdly, when my son and I were coming back from his school carol concert a few days ago we saw another shooting star.
It was wonderful, as I hadn't seen one again since that night when I was very little."
I love O Holy Night and have done since childhood. I used to be part of a young people's group in church and it was one of the songs we performed. Also, when I was in the choir at high school it was one of the carols we sang in sixth year. I particularly liked it because I got to sing one of the solos.
I love this song when it's sung by a choir - the Belfast Community Gospel Choir does a great version of it with harmonies which make you feel all warm and tingly and Christmassy. People only ever remember the first verse, but the words to the other verses are just lovely too."
Chris Rea's Driving Home For Christmas resonates with me this year as my oldest son is travelling home from London where he now lives.
It's an old song that's been around for a while.
I'm making all sorts of plans to pick Elliot up from the airport and welcome him home, so he's not driving as such.
When your kids grow up and move out, the songs that you loved when you were much younger, but didn't realise the significance of, start to mean something.
I love hearing Christmas songs on the radio and in shops, because that's when I start getting those Christmassy feelings."
My childhood memory of Christmas is that selection boxes had full-sized versions of chocolate bars so you got lots of stuff in them.
I once got a selection box with a cassette tape in it full of Christmas songs - nowadays it would be a download code or a memory stick, but a cassette tape seemed much more personal.
That tape was played constantly, but it's Cliff Richard's song Mistletoe & Wine that always reminds me of that time in my life.
It also reminds me of my granny, who was into Christmas in big way - whenever I hear that song I think of her."
Christmas songs are like a form of torture sometimes! Although we only hear them once a year, they're repeated over and over again until we're thoroughly sick of them.
Carols have more longevity, not least because you don't hear them on the radio incessantly. I love Silent Night, particularly if it's sung in the original German.
The best time to hear it is at Christmas mass, when they dim the lights and it feels that something special is happening.
When the boys were small and had gone to bed and we would be up to help Santa out, we would listen to carols.
One year I was up until 1am assembling a toy pirate ship and I put on the carols from King's on the TV. Now it's a Christmas Eve tradition for us. A solo singer at King's always sings the first verse of Once in Royal David's City, but they don't know who it will be until just before they go on, so they've all been trained to do it."
I love Christmas songs, as they are such an important part of the season.
My favourite one has to be Fairytale of New York, with Kirsty McColl and The Pogues. This song was in the charts when I was a teenager.
You knew you were at the Christmas disco in the youth club when you heard that coming on. I remember me and my mates charging round the dance floor to that. When I hear that song, I know that it's Christmas."
If you're in need of a feel-good watch, get on Netflix and load up Someone Great. Written and directed by Jennifer Kaytin Robinson, the film follows music journalist Jenny, played by Gina Rodriguez, the lead star of US hit TV series Jane The Virgin.
Few roles have transformed an actor's career as much as Thor has transformed Chris Hemsworth's. The Australian heartthrob was still best known for his role as Kim Hyde in Home And Away when he was first cast as the God Of Thunder in 2009, at the age of 25.


text:
Berlin Film Festival 2012
Loco London Comedy Film Festival
London Film Festival 2010
London Gay & Lesbian Film Festival 2010
Sundance London 2016
sodomfilm.com
★★★★, Sundance, USA June 3, 2016
Other People (2016)
By Alexa Dalby
A semi-autobiographical story of comedy in the heart of tragedy, Chris Kelly's Other People sees both good things and bad happen to us all.
★★★, thriller, USA May 2, 2014
Blue Ruin (2013)
A big hit at Robert Redford's Sundance Festival in the US and previewed at Sundance London, this suspenseful, original and darkly comic revenge thriller set in America's South is hugely enjoyable.
features January 4, 2014
Review Of The Year: 2013
By Mark Wilshin
In black and white or riotous colour, here's a quick look back over the best and worst films of 2013 and a sneak preview of the movies to watch out for in 2014.
★★★★★, comedy, USA December 6, 2013
Nebraska (2013)
Determined to pick up a nonexistent million-dollar mailshot prize, an elderly father on the edge of dementia is driven across America by his long-suffering son.
festivals, London Film Festival 2013 October 20, 2013
Film Festival: The 57th London Film Festival 2013
The 57th London Film Festival 2013 by Mark Wilshin We've travelled the ocean's waves, gone back in time, into outer space and all across…
Blog, London Film Festival 2013 October 13, 2013
London Film Festival 2013 – Day 5
Freedom, obsession and discrimination, it's all here. And in Poland, Iran, Bosnia-Herzegovina, Sweden and South Africa. First, there's Jafar Panahi's Closed Curtain, his fictional…
The vogue for monochrome continues with Alexander Payne's Nebraska. And after Hawaii in The Descendants, Payne ups sticks to another overlooked state, this time…
Blog, London Film Festival 2013 October 9, 2013
London Film Festival: Captain Phillips Opening Night
The London Film Festival opens tonight with Captain Phillips, Tom Hanks bookending the festival with Saving Mr Banks for the closing night gala. And…
The Dead Don't Die (2019)
Vita and Virginia (2018)
★★★★, documentary, out now, Reviews, Sundance London 2019, USA	The Brink (2019)
★★★, Cannes Film Festival 2019, comedy, horror, out now, USA	The Dead Don't Die (2019)
★★, biopic, Ireland, out now, UK	Vita and Virginia (2018)
Mark Wilshin
James Reed
Gus Edgar
Chris Drew
About Dog And Wolf
Dog And Wolf is an independent, online film review magazine bringing you the latest in world cinema and the best of the film festival.
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The RECTOR'S REPORT was delivered at both the 8:00 and the 9:30 am services.
Warden Jennifer Pike reported on the recommendations of the Nominating Committee.
The Committee consisted of myself, as Warden, Pat Mohlere, as the Vestry representative, and Katie Plumb, as the Parishioners' representative.
Lynda Bradley and Crosby Renwick have graciously agreed to stand for a second term.
Alex Coulter has been nominated to the position of Warden.
The two vacancies created by Alex's promotion and Anne Curtain's retirement lead us to nominate Patty Bigelow and Charlie Williams.
There being no further nominations from the floor the recommendations of the Nominating Committee were accepted unanimously.
"A true picture of what St. Ann's is all about, certainly came into focus this Spring when Tim had to have emergency surgery in Greenville, NC. In his absence, you all stepped up to the plate to keep us in faith for so many weeks. It may have been a comforting conversation Jen & I shared with you during coffee hour; or diligently attending to our usual business of "doing our jobs" or actually leading us in prayer. (Sandy Taylor & "Father" Rick Conklin.) For all of us, it was a bonding experience. A time of continued support. Many thanks.
We're grateful to have you back at the pulpit Tim, feeling 101%.
It is with sadness that we have to let Anne Curtin go off the Vestry. She has with great dedication, been a member for 2 terms, and can't actually do "any more time!" Rules are rules.
Jen Pike has also finished her Wardenship. This has truly been a great partnership to work with one of my best buddies, on close to a daily basis. A number of years ago, Jen and I were walking along Long Beach with our doggies, and I was sharing my drama as to "who was going to be Warden with me, now that Beth George was heading south." Jen said with such commitment, "Well I'll do it!" I thought she was joking, and then I soon realized that she was serious. You can imagine my glee! We've continued to share some laughs along the way…. and it comes with great thanks that she was our Warden. Luckily, she isn't going to go far.
And now, Alex Coulter is joining me as the next Warden. I am looking forward to working with another dedicated member of this church. His new position will not be getting him off the hook when moving Christina's holiday boxes up and down the Parish Hall.
Andy Steffens continues to be the pillar of this church. He quietly is in the know and is always willing to put together our financials and as Clerk, takes the best notes at most Vestry Meetings. Vestry Minutes are typed up and sent to us for approval sometimes before we barely arrived home from church that day! What would we do without you, Andy!
Once again, promoting our gorgeous St. Ann's Christmas Ornaments. Only $20.
Here Vestry Members stood up to be recognized.
Treasurer Andy Steffan delivered his reports.
There being no further business the Annual Meeting adjourned.


This is in response to the question, "What happened to your essential oil company?" I launched this company in December 2017 and closed it one year later, in mid January 2018.
I value transparency in my communication with you, as well as vulnerability in sharing my own healing journey with you. That has always been a foundation of Empowered Sustenance, and will continue to be.
Last week, I went through a 15-hour non-stop legal negotiation. Although it was one of the longest days in my life, I remained peaceful about the outcome.
Because I've learned to trust the truth to take care of me.
And it did.The essential oil company launched last year with a business partner closed yesterday. Why?
This relationship began when my nutrition and bodywork practitioner, 38 years my senior, seduced me when I was his 21-year-old client. I was vulnerable for many reasons, including a lack of any prior relationship and sexual experience. I also deeply trusted this man, who was a paragon in my community. I naively entered a relationship with him. And, shortly after, I naively agreed to start a business with him.
When I realized the truth of the relationship, I chose to end the business because I no longer wanted connection with this man. I also chose to report him to the appropriate authorities, and now many other women whom he mistreated are speaking up in the multiple investigations.
Lastly, I chose to voice the abusive relationship dynamic I experienced in my poetry book, Stronger Than The Master, in order to create positive change in our culture.
I know this change needs to occur, as I am watching other women in my life go through parallel circumstances. They are also extricating themselves from manipulative partnerships — in business and marriage — and then experiencing the same pattern of escalated harassment, threats, false accusations, and legal challenges. And then there is the painful realization that some friends will choose to believe the abuser.
At Empowered Sustenance, I share what empowers and sustains my healing journey. This means that I am vulnerable and honest in sharing my own journey. And this entire experience has been a significant part of my healing journey.
I am glad I could support you with this essential oil company.
I know that I will support your healing in new ways in the future.
I trust that doors close so new ones can open.
The post "What happened to your essential oil company?" appeared first on Empowered Sustenance.


When Colton King's marriage to Penny ended after just 24 hours, he didn't expect a year later to discover two little secrets: a baby boy and a baby girl. Now, after claiming his twins and reclaiming Penny, he must ask himself: was their whirlwind marriage meant to last a lifetime?
Despite his fiancée's betrayal, developer Ryan Beardsley provided for Kelly when he sent her packing. So he's livid to find her working in a dingy diner, desperate and pregnant. Whether the baby is his - or his brother's - Ryan demands that Kelly return to New York with him. For the baby's sake. Or maybe because Kelly is as irresistible as ever.
She's kept her baby secret for two years. But even after a chance run-in forces her to confront the father of her son, Sabine Hayes refuses to give in to all of Gavin Brooks' demands. She'll let him get to know Jared, but she won't go back to Gavin's privileged world.
Gavin never stopped wanting the woman who challenged him at every turn. He'll do just about anything to stop her getting away again.


Mother's Day will be here before you know it so come to the Stephentown Memorial Library and make Mom a special Mother's Day gift and card that she will be sure to love. Maria will help your child to make something special. Registration is not required but would be appreciated, 518-733-5750.


text:
El Bronce de Audita es un tratado de hospitalidad latino de enorme valor arqueológico e histórico. Es uno de los cuatro hallados en la provincia de Cádiz; los otros son los de Bonanza, Iptuci y Lascuta.

Localización
Se encontró en 1765 en el peñón de Audita, cerca de Lacilbula. Esta tabula hospitii del año 5d.C. refleja el pacto con el senado de la ciudad de Lacilbula. Actualmente se encuentra en el Museo Arqueológico Nacional, en Madrid.

Enlaces externos
 Bronce de Audita.

Epigrafía de la Hispania romana
Historia de la provincia de Cádiz
Iberia
Bética
Placas de bronce

redpajama_set_name: RedPajamaWikipedia


text:
Avustajille
Tilaus ja jakelu
Paska kotiseutu
Kasvo
Jyväskylän Ylioppilaslehti
Jyväskylän ylioppilaslehden verkkojulkaisu
Ei hakutuloksia.
Teksti Petra Nykänen
Education at stake in parliamentary elections
In English | 27.03.2015
Finland, as a country of free higher education, could be at risk. The result of the parliamentary elections in April might be decisive for whether or not tuition fees will be introduced for international students.
The Finnish parliamentary elections will be held on April 19. In total, 200 representatives will be selected to the Parliament.
The whole country is taken over with election buzz. You have probably come across a campaigner on the street, seen election billboards pretty much everywhere, and witnessed politicians arguing on TV.
So, what is it about? And moreover, what is it to you?
The current economic downturn is said to be the longest one in Finland's history. This means that the government is forced to make major budget cuts.
Well, also education is on the cutting board.
The current government proposed massive cuts in education, says Jari Järvenpää, the president of The National Union of University Students in Finland.
"We could be talking about as much as two billion euros", he estimates.
"It depends on whom you ask of course, but in any case, it is clear that hundreds of millions have been already cut in education."
The impact of the cuts can be seen, for instance, in the Finnish Student Health Service. Due to the cuts, its service charges increased last year. They also affect the Finnish study grants.
International students could be a target as well.
Last autumn, Krista Kiuru, the Minister of Education, Science and Communication, proposed tuition fees for higher education students, who come to study in Finland from countries outside the European Union and the European Economic Area. Per student, the fee for one academic year would be at least 4,000 euros.
Kiuru later withdrew the proposal. However, if the new government decides to support the fees, they could become a reality after all. Perhaps already in a few years time, adds Järvenpää.
Therefore, he thinks the result of the elections to be crucial.
"Political parties have very different outlooks on the matter", Järvenpää points out.
"For example, the Centre Party and the National Coalition Party have expressed their support for the fees, whereas the Greens and the Left Alliance are against them. There are also parties who have not yet decided their stand on the matter."
Finnish universities and universities of applied sciences already tested the use of tuition fees for international students, reminds Järvenpää.
He refers to the Ministry of Education and Culture's four-year trial (2010–2014), which gave the higher education institutions a chance to charge non-EU and non-EEA higher academic degree students.
"Technically, the new law would be easy to realize since it has already been tried out. Of course, the trial turned out unsuccessful, but that doesn't seem to concern the decision makers."
kiuru
Jaa Twitterissä:
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Jaa Telegrammissa:
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Only five students pay for their studies
Coming from Europe, going to Europe
An "OK" Grade for Student Health
© 2020 Jyväskylän ylioppilaslehti
ISSN 2341-7218 (painettu)
ISSN 2341-7226 (verkkojulkaisu)


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Sheree Wright and IBF Law Group Help Migrants as They Venture into the United States
As the decade comes to a close, we can look back and think of important factors that marked our history. One of those factors is immigration; globally caused by war or crises, and nationally caused by increased persecution in Central and South America and the wish for a better life here in the United States. Nonetheless, it is a hard time to be a migrant, as many see migrants as the enemy. That is why the work of lawyers like vz is crucial.
Sheree Wright is a founding member of IBF Law Group. She graduated from DePaul University in 2013 with a Bachelor of Science in Political Science and later pursued a Juris Doctorate and a Masters of Law. During her undergraduate career, she was an investigator with the Equal Employment Opportunity Commission (EEOC) in downtown Chicago.
"My main focus at the EEOC was to investigate cases that involved employment discrimination on the basis of age, race, sex, gender or disabilities in Fortune 500 companies." Sheree recalls. "Then during my law school career, I co-founded a non-profit organization called The Wright Way Foundation."
The Wright Way Foundation seeks to reduce poverty and homelessness in Jamaica, while generating and providing educational resources in order to support youth, families, groups and educators in the Caribbean country.
"Through its projects, the foundation seeks to sustain interest in the financial development of underserved youths with practical resources and hands-on experience that will enable them to pursue their own future interests." Sheree says.
The Wright Way Foundation makes a valuable contribution to civil society in Jamaica, especially with regard to social and educational outreach projects with local orphanages and educational institutions. Sheree also became a volunteer of Mi Familia Vota, a national civic engagement organization that unites Latinos, immigrants and allied communities to promote social and economic justice through citizen workshops, voter registration and voter participation.
With IBF Law Group PLLC, a law firm located in downtown Phoenix, Sheree is able to merge all her experience together. As an immigrant herself, she realized that immigration law was her true passion.
"I started to notice the lack of genuine immigration attorneys in the field. I am an immigrant and so my passion for immigration law is natural. I got into this business because I was passionate about it, and I would advise people to find a field that they are passionate about so that it does not feel like work." Sheree shares.
When getting started with the firm, one of the challenges was finding like minded people who understand the importance of timeframe, deadlines and immigration law in general. To get through this and get work done, mindset is very important.
After finding some success through her firm, Sheree is able to see that "fear is imaginary" and it hinders a person to move forward. She also now sees success as not about financial gain, but about helping the less fortunate.
This is why her next projects include going to the southern border of the United States and volunteering with migrant children. For her, financial freedom means "having enough to give back to her community."
"The biggest obstacle that I have had to overcome, and still work on, is my emotion. Immigration law is a very heart-wrenching area of practice, especially during this administration where there are so many new laws being implemented to stop or hinder migrants to cross the border." Sheree states. "These are usually migrants who are running from persecution in their home country and their cases are life or death."
This is also how Sheree differentiates herself from her competition–she actually cares and has seen the results of her hard work. To follow her work and that of IBF Law Group, go here.


text:
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Suspects Season 5
The next season of the British police procedural series, Suspects, a show that is centered on police actions and also doesn't shy away from portraying controversial actions such as corruption and abuse of power, will air next Spring. Critically acclaimed series feature actors like Fay Ripley, Damien Molony, Clare-Hope Ashitey, James Murray and others. Viewers that enjoy a well-made British detective are advised to at least try this series, as it is highly satisfactory.
Event date: 2017-03-01 01:00:00 PM
Creation date:2016-07-11 11:52:08 AM
Cyberpunk-themed action role-playing video game, an heir to one of the most famous game franchises, and a sequel to 2011 Deus Ex: Human Revolution is almost here. It will deal with issues like human augmentation, technological and social change. The protagonist, Adam Jensen, will work as an cyborg crime investigator, while simultaneously seeking to destroy conspiracy against augmented people. If previous game is any indicator, Mankind Divided will feature many different character development, gameplay and moral choices in the process.
Creation date:2016-07-11 12:27:26 PM
Crysis 4 release
A promised conclusion to an epic tale of nanoarmor-clad supersoldiers, Crysis franchise returns with a fast-paced action video game, filled with high-quality graphics and exciting story. Game developers refused to call it Crysis 4, since it is supposed to be the final game of the series, and it will come with a lot of changes to its time-proven formula. Call it however you want, but deliver us more of this amazing technothrill! We are waiting, Crytek!
A fourth game in the Mass Effect series, this team-oriented sci-fi rpg shooter will be released in the first quarter of 2017. The game setting involves humanity acting in a galaxy full of advanced alien races, complicated politics and unexpected dangers of truly cosmic proportions. The game will reportedly take place in an open world setting, full of choices and resources.
Taboo series release
A historical drama television series is about 19 century colonialism, crime and revenge.It features such stars as Tom Hardy and Johnathan Pryce, and it will be directed by Kristoffer Nyholm and Anders Engstrom, two experienced Nordic criminal show producers. The premise and the cast are very interesting, and I intend to wait for more information on this show. It is supposed to air on 2016.
Halo Wars 2 release
Halo Wars 2 is an upcoming real time strategy video game, taking place after the events of Halo Wars. A player can choose between two factions, Humans and Banished. Spirit of Fire and its crew will return to face new challenges, and races of Covenant have rearranged themselves into a new collective. Game will be released on Microsoft Windows and Xbox One platforms.
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