Belle-Anse is one the poorest cities in Haiti, and many people survive by fishing. The town sells fish to other nearby towns and Port-au-Prince.
Belle-Anse is one the poorest cities in Haiti, and many people survive by fishing. The town sells fish to other nearby towns and Port-au-Prince. During the 60's and 70, there was a group of middlemen who did very well by buying coffee from the local peasants and selling it to exporters in Jacmel and Port-au-Prince.
There are several elementary schools, one government run, and a few other ones that have been established through partnerships with foreign organizations. Education beyond an elementary level remains, however, a challenge. For many years, L'Ecole Vocationelle de Belle-Anse was the only means to obtain a partial secondary education and that school focuses on trade skills, instead of academic subjects. Those who wish to obtain a solid secondary school education must travel to Port-au-Prince or Jacmel. A complete and solid secondary education still remains a need and a challenge for many Belle-Anse.
A clinic provides basic medical care for Belle-Anse and a hospital remains far away. The clinic lack sufficient staffing, with only one nurse most of the time. People rely on the St. Michel Hospital in Jacmel for acute medical care. In some circumstances, it is the local people who provides medical care by using their knowledge of natural medicine.
NS4HS has a strong track record of proven successes, accountability and in the past five years, despite limited financial and logistical means. We have been able to establish and maintain a strong, reliable partnership with the Haitian community of Belle-Anse. This partnership offers both regions an opportunity to build strong relations. NB4HS with Rhode Island donors are in the process of building a new medical facility to help elevate clinic conditions. Beyond our construction work with the clinic, we continue our commitment of leading efforts for fair and just programming aimed at providing scholarships, tuition fees, uniforms, school supplies, and teaching materials within the school system. In addition to our health and education work, we are also helping with the collection and distribution of food.


text:
Robin Lehner
Photo: Nicole Sweet - USA TODAY Sports
Ristolainen is no longer with Scandella
Buffalo, NY (WGR 550) - With the poor play of Rasmus Ristolainen in the last four games, Phil Housley feels he has to change up the D-pairs. This morning it was: Scandella - Nelson Beauleau - Ristolainen Gorges - Antipin Wilson - Falk Ristolainen struggled mightily against the Ducks and Phil...
Photo courtesy of @buffalosabres (Bill Wippert)
Nolan glad to be a part of Hockey is for Everyone
Buffalo, NY (WGR 550) - In the month of February, the NHL is honoring everybody who wants to play hockey with their Hockey is for Everyone campaign. On Tuesday, the Sabres honored those who want to play hockey. Members of the Buffalo Beauts were in attendance as were others who want to play. The...
Pat Malacaro
Ducks defeat Sabres 4-3 in OT
Ryan O'Reilly tied the game with just seconds to go in regulation, but an overtime goal by Adam Henrique lifted the Anaheim Ducks to a 4-3 win over the Buffalo Sabres on Tuesday night at KeyBank Center. Here's how the game went down: First Period: 17:37 - Rasmus Ristolainen takes the first minor...
Photo: Charles LeClaire - USA TODAY Sports
The Sabres are back to practice trying to figure out their own arena
Buffalo, NY (WGR 550) - The Sabres know the situation they're in and they know it's from their own doing. Buffalo is 6-17 in one goal games this year and that number would be a lot higher if you took out empty net goals. Jack Eichel is frustrated by it, he said, "It's part of it. You want to get to...
From the Locker Room: Sabres-Blues
The Buffalo Sabres played host to the St. Louis Blues on Saturday night at KeyBank Center. This was the first of two games that the Sabres were without forward Johan Larsson, who is currently serving a two-game suspension for a cross-check to the head of Florida Panthers' forward Vincent Trocheck...
Sabres shut out by Blues 1-0
The Buffalo Sabres' home woes continue as Carter Hutton makes 27 saves for the St. Louis Blues to help the team to a 1-0 win on Saturday night at KeyBank Center. Here's how everything broke down: First Period: 14:40 - Both teams have been going end-to-end with the play so far, Buffalo heads to the...
Sabres score late, New Jersey holds on for 3-1 win
Jake McCabe scored on the power play for the Buffalo Sabres late in the third period, but it was not enough to extend their three-game winning streak. The New Jersey Devils beat Buffalo 3-1 on Tuesday night at KeyBank Center. Here's how the game broke down: First Period: 20:00 - It has felt like...
Photo: Kevin Hoffman - USA TODAY Sports
Lehner wants all the praise to go to his teammates
Buffalo, NY (WGR 550) - After a wildly successful Western Canada road trip, the Buffalo Sabres return home trying to make up for a 7-1 loss to Dallas. Buffalo won all three games on the trip and are now 8-14-7 away from home. That's a .397 points percentage in 29 games. The Sabres have only played...
Photo: Reinhold Matay - USA TODAY Sports
Eichel is back from a fun All-Star weekend
Buffalo, NY (WGR 550) - Jack Eichel was the only Sabres representative at the NHL All-Star weekend in Tampa. In the first game, Eichel had a goal and two assists, but was held off the score sheet in the second game. Through it all, Eichel had a blast, "It was a good one, it was a new experience and...
Eichel and Lehner earn league honors
Buffalo, NY (WGR 550) - For the first time in team history, the Buffalo Sabres have two of the NHL's Three Stars of the week. Jack Eichel is the league's second star. Eichel led the NHL with two goals and three assists for five points in the three wins out west. Eichel scored the overtime game-...

redpajama_set_name: RedPajamaCommonCrawl


text:
Gyrinus colymbus ist eine Käferart aus der Familie der Taumelkäfer (Gyrinidae).

Merkmale
Die Käfer erreichen eine Körperlänge von 5,5 bis 7 Millimetern. Die Deckflügel sind am Grunde sehr fein chagriniert (Narbenmuster) oder sehr eng punktförmig strukturiert. Die Epipleuren am Halsschild und den Deckflügeln sind ganz oder teilweise gelb gefärbt, die Beine sind immer gelb. Das letzte Hinterleibssegment ist rostfarben.

Vorkommen
Die Art ist vor allem in Südosteuropa verbreitet. Sie kommt in Ungarn, Tschechien, der Slowakei, auf dem Balkan und teilweise auch in Mitteleuropa und bis nach Norditalien und Südostfrankreich vor.

Quellen

Literatur
 Adolf Horion: Faunistik der mitteleuropäischen Käfer Band I: Adephaga - Caraboidea, Hans goecke Verlag, Krefeld, 1941.
 Edmund Reitter: Fauna Germanica – Die Käfer des Deutschen Reiches. 5 Bände, Stuttgart K. G. Lutz 1908–1916, Digitale Bibliothek Band 134, Directmedia Publishing GmbH, Berlin 2006, ISBN 3-898-53534-7

Taumelkäfer
Gyrinidae

redpajama_set_name: RedPajamaWikipedia


text:
lisa's story begins
Tom Batiuk
The Kent State University Press
Prelude is a collection of the early comic strips that bring Lisa and Les together. Introduced to readers of Funky Winkerbean in late 1984 as she experiences SAT test anxiety, Lisa becomes Les Moore's best friend and a pivotal character. Les and Lisa go to the prom, begin steady dating, and then break up. Over the summer, Les realizes how much he misses Lisa. When he gathers his courage and goes to her house, he is stunned to discover Lisa is pregnant with a child fathered by a jock from Walnut Tech. Lisa asks Les to be her coach in childbirth classes, and their friendship explodes from there. Prelude takes fans from the early days of their deep friendship through the birth of Lisa's baby and the baby's adoption.
Tom Batiuk, who pens the continuing story of Funky Winkerbean and his pals, was recognized as one of three finalists in the cartooning category of the 2008 Pulitzer Prize awards competition for the series of daily comic strips that chronicled the death of longtime character Lisa Moore. His Complete Funky Winkerbean series and Roses in December: A Story of Love and Alzheimer's were finalists for the 2016 Eisner Awards. He is a graduate of Kent State University. His Funky Winkerbean and Crankshaft comic strips are carried in over 700 newspapers throughout the United States. In 2006 he was honored by the American Cancer Society and presented its Cancer Cure Hall of Fame Award for his sympathetic work in highlighting the experiences of those with cancer in Lisa's Story: The Other Shoe.
Other Titles from The Kent State University Press
Media, Profit,and Politcs
As Ohio Goes
The Weary Boys
Confederate Tide Rising


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text:
Frontiers in Physiology
Kevin I. Watt
The University of Melbourne, Australia
Daniel L. Clark
The Ohio State University, United States
Richard Jaspers
Vrije Universiteit Amsterdam, Netherlands
Front. Physiol., 27 February 2018 | https://doi.org/10.3389/fphys.2018.00152
Differing Effects of Younger and Older Human Plasma on C2C12 Myocytes in Vitro
Ifigeneia Kalampouka1, Angel van Bekhoven1,2 and Bradley T. Elliott1*
1Translational Physiology Research Group, Faculty of Science & Technology, University of Westminster, London, United Kingdom
2Engineering and Applied Science, Hogeschool Rotterdam, Rotterdam, Netherlands
Ageing is associated with a general reduction of physiological function and a reduction of muscle mass and strength. Endocrine factors such as myostatin, activin A, growth and differentiation factor 11 (GDF-11) and their inhibitory peptides influence muscle mass in health and disease. We hypothesised that myocytes cultured in plasma from older and younger individuals would show an ageing effect, with reduced proliferation and differentiation in older environments. C2C12 myoblasts were grown as standard and stimulated with media conditioned with 5% plasma from healthy male participants that were either younger (n = 6, 18–35 years of age) or older (n = 6, >57 years of age). Concentration of plasma myostatin (total and free), follistatin-like binding protein (FLRG), GDF-11 and activin A were quantified by ELISA. Both FLRG and activin A were elevated in older individuals (109.6 and 35.1% increase, respectively), whilst myostatin (free and total) and GDF-11 were not. Results indicated that plasma activin A and FLRG were increased in older vs. younger participants, GDF11 and myostatin did not differ. Myoblasts in vitro showed no difference in proliferation rate between ages, however scratch closure was greater in younger vs. older plasma stimulated myoblasts (78.2 vs. 87.2% of baseline scratch diameter, respectively). Myotube diameters were larger in cells stimulated with younger plasma than with older at 24 and 48 h, but not at 2 h. A significant negative correlation was noted between in vivo plasma FLRG concentration and in vitro myotube diameter 48 h following plasma stimulation (r2 = 0.392, p = 0.030). Here we show that myoblasts and myotubes cultured in media conditioned with plasma from younger or older individuals show an ageing effect, and further this effect moderately correlates with circulating FLRG concentration in vivo. The effect of ageing on muscle function may not be innate to the tissue, but involve a general cellular environment change. Further work is needed to examine the effect of increased FLRG concentration on muscle function in ageing populations.
Human ageing is associated with a general decline in physiological function from the 3rd decade onwards, including a loss of muscle mass and strength (Kallman et al., 1990). This reduction in muscle mass and function is linked with reductions of independence, reduced metabolic health, and an increase in all-cause mortality (Newman et al., 2001; Puts et al., 2005). Western society is experiencing an ageing population, with an increasing proportion of society >65 years of age, and thus an increasing need for understanding and treatment of the ageing-related loss of muscle mass and function (Bloom et al., 2015).
This loss of muscle function does not result in muscle tissue losing its plasticity and ability to respond to anabolic stimuli. It is well-established that older individuals respond adequately to resistance training with gains in muscle mass and strength (Harridge et al., 1999; Liu and Latham, 2009). The effect of ageing on total satellite cell number within muscles is unclear to date (reviewed by Snijders et al., 2009). Reduced proliferation rates are seen in satellite cells sourced from older muscle in mice (Chakravarthy et al., 2000). However, primary cell culture from biopsies of younger or older individuals do not differ in their proliferative rate (Alsharidah et al., 2013), but may show reduced fusion capacity when cultured in vitro (Pietrangelo et al., 2009; Brzeszczynska et al., 2018). Further, when age-dependent satellite cell proliferation capacity is seen in mice, the provision of insulin-like growth factor (IGF) is capable of off-setting this (Chakravarthy et al., 2000). These results suggest that the muscle cells per-se maintain functionality in ageing, but the systemic environment may instead be key.
Indeed, ageing is associated with changes in circulating factors that are known to moderate muscle mass, including the growth factors testosterone, growth hormone, and IGF (Ratkevicius et al., 2011; Sellami et al., 2017) and pro-inflammatory cytokines (Ferrucci et al., 2005; Alvarez-Rodríguez et al., 2012). The transforming growth factor beta (TGF-β) family of signalling peptides is relatively less studied. Myostatin (or growth and differentiation factor 8, GDF8) is a TGF-β peptide family member that acts as a negative regulator of muscle mass (McPherron et al., 1997). Myostatin circulates in an endocrine manner (Zimmers et al., 2002), and this endocrine compartment of myostatin correlates with muscle mass in humans across health and disease states (Gonzalez-Cadavid et al., 1998). Closely related peptide family members include activin A and growth and differentiation family member 11 (GDF11). Indeed, GDF11's amino acid similarity is ~90% relative to myostatin, despite GDF11 acting on a wider range of tissues than myostatin, including anterior posterior patterning during development, visceral organ development and neural development (Walker et al., 2016). Due in part to their structural similarity, circulating myostatin, activin A and GDF11 share inhibitory binding proteins, including follistatin, follistatin-like binding protein (FRLG) and GDF-associated serum proteins 1 and 2 (GASP-1/2; Nakamura et al., 1990; Hill et al., 2002; Amthor et al., 2004; Lee and Lee, 2013).
Plasma myostatin peptide concentration does not appear to change with age, despite older individuals showing reduced muscle mass (Ratkevicius et al., 2011). Mixed reports exist as to the changes in GDF11 with age, with one report of an increase with age (Egerman et al., 2015), whilst another reports a decrease (Sinha et al., 2014). We recently reported that chronically highly active older individuals showed increased GDF11 concentration over sedentary, aged matched controls (Elliott et al., 2017).
If the ageing process induces differences in these endocrine factors and their inhibitory binding proteins, then this may explain the loss of muscle mass and function with increasing age. Thus, the aim of this experiment was to establish the effect of cellular external environment on myocyte homeostasis in vitro. It was hypothesised that myocytes in an older endocrine environment would show reduced proliferative and growth response, relative to those in a younger environment.
The protocol was approved by the University of Westminster Research Ethics sub-Committee. All participants gave written informed consent in accordance with the Declaration of Helsinki prior to their participation. Participants (n = 12) were male, declared healthy, and equally divided into younger (n = 6, 18–35 years of age), or older (n = 6, >57 years of age. Age was recorded to the nearest month (recorded in a decimal format, e.g., 52 years 6 months = 52.5 years). Exclusion criteria including regular (daily) smoking, clinically unstable conditions, and a BMI < 18 or > 30.
Participants were requested to avoid strenuous activity for 24 h and fast for 12 h prior to the morning of testing. Participants presented at the research facility between 08:00 and 11:00 for testing. Following measurement of height and weight (287 dp, Seca, Germany) in minimal clothing, fat mass (FM), fat free mass (FFM), and skeletal muscle mass (SkM) was quantified by bioelectric impedance (mBCA 515, Seca, Germany). Participant characteristics are summarised in Table 1.
Table 1. Participant characteristics, grouped into younger (n = 6) and older (n = 6).
Approximately 12 mL of whole blood was then collected via a convenient cubital vein in a standard manner into lithium heparin coated tubes. Collected whole blood was immediately spun (4°C, 5 000 RPM, 10 min) and resultant plasma was aliquoted and stored at −80°C for further testing.
Physical functionality of participants was assessed by hand grip dynamometry (T.K.K. 5401, Takei Scientific Instruments, Japan) and a 6 min walk test (6MWT). For grip strength, each participant stood with their dominant arm hanging straight and semi-pronated (palm facing medially). Participants were asked to give a maximal effort with verbal encouragement for 3 s, with 60 s between efforts. Grip strength was recorded three times, with the maximal value reported here. The 6MWT was performed in a standard manner (ATS Committee on Proficiency Standards for Clinical Pulmonary Function Laboratories, 2002) on a pre-marked indoor track. A hand counter was used to record distance, measured to the nearest meter.
Enzyme-Linked Immunosorbent Assays
Concentration of circulating myostatin within plasma (free and total, DGDF80), activin A (DAC00B), FLRG (DFLRG0) and GDF11 (DY1958) were measured by commercial ELISA (all R&D Systems, UK). Briefly, plasma samples were brought to room temperature before analysis. Total myostatin was measured by acidification of plasma samples (100 μL plasma incubated with 50 μL 1 M HCI for 10 min at room temperature, then 50 μL of 1.2M NaOH/0.5 M HEPES) and finally 200 μL calibrator diluent (RD5-26) for a 1:4 dilution factor, whilst plasma samples for free myostatin was diluted 1:4 in calibrator diluent, before resultant mix was loaded at 100 μL per well, as we describe previously (Elliott et al., 2017). Activin A, FLRG and GDF11 were measured with 100 μL of plasma loaded per well. Incubation times, conditions and washes followed manufacturer's instructions. All plates were read at 450 nm and blanked to 570 nm (FLUROstar Optima, BMG Labtech, UK). Recombinant myostatin (894410), activin A (890709), FLRG (894136), and GDF11 (844380) was used as standards, all provided by R&D (UK). All samples and standards were measured in triplicates.
Myoblasts from the established C2C12 cell line were seeded (passage 11) into T75 flasks in triplicate in growth media (GM; Dulbecco's modified Eagle media with 10% fetal bovine serum, penicillin and streptomycin) in standard conditions (37°C, 5% CO2,100% humidity) with media changed every 48 h until ~ 80% confluent for seeding into experimental conditions. All experimental plates (described below) were seeded at an initial density of ~3.0 × 104 cells.mL−1. All in vitro experiments described hereafter were performed with an n = 6 per group (young or old) with each individual run in triplicate.
Cell Proliferation
To assess the effect of younger or older plasma on myoblast proliferation, cells were seeded in 6 well plates in GM + 5% plasma (younger or older). Cell proliferation was assessed at 24 and 48 h. Briefly, following aspiration of media and washing with dPBS, cells were trypsinized (TrypLE, Gibco, 12605) for 5 min at 37°C to ensure complete cell removal, then resuspended. Cell counts per well was measured in duplicate, utilising an automated cell counter (Cell Countess II, Thermo Fisher, UK).
Scratch Assay
To assess the effect of younger or older plasma on myoblast migration, cells were seeded in 6 well plates in GM, and proliferated as standard until >80%. Scratch assays were performed as described by Liang et al. (2007). Briefly, cells were scratched once in a single, fluid motion using a sterile p200 tip to remove a linear section of cells. Cells were then washed with dPBS, before the addition of GM + 5% plasma (younger or older). Cells were immediately photographed (10x, 1.3 megapixel digital camera) in 3 random locations per scratch, then incubated in standard conditions for 4 h before repeating photographs. Scratch size was quantified in ImageJ (version 1.49, NIH, USA) by an individual blinded to condition and time, and closure calculated as percent of individual baseline.
Myotube Diameter
Following seeding in 6 well plates, myoblasts were grown to ~80% confluency as standard. Near confluent cells were switched to differentiation media (DM; Dulbecco's modified Eagle media with 2% horse serum, penicillin, and streptomycin), with DM changed every 24 h for 96 h. Following this, cells were stimulated with DM + 5% plasma (younger/older), and photographed immediately in 3 random locations per plate, as described above. Cells were further photographed at 4, 24, and 48 h in the same manner. Cell diameter was quantified at three different locations along the cells length, in 10 random cells per image, by an individual blinded to condition and time utilising ImageJ.
Normality of data was checked for by Shapiro–Wilk test. Differences between younger and older characteristics, Activin A and FLRG ELISA concentrations, and scratch assay results were established by two-tailed, unpaired t-tests, whist effect of age on GDF11 was established by a Mann Whitney test. Concentration of total and free myostatin, cell proliferation, and myotube diameters were compared with a mixed-model two-way repeated measures ANOVA, with post hoc testing performed in the method of Bonferroni when indicated. Pearson's correlation was used to examine associations between endocrine factors that differed between younger and older individuals and in vitro results. SPSS (version 23, IBM) was used for all statistical analysis, and all figures were prepared in GraphPad Prism (version 5, GraphPad Software). Results are given as "mean [se]", except GDF11, which is given as "median [lower–upper quartile]."
FLRG Differs between Younger and Older Individuals
To establish the effect of age on the concentration of myostatin and myostatin-interacting peptides, plasma from younger and older individuals was used. A two-way mixed model (age × peptide state) ANOVA showed no interaction for myostatin (p = 0.610), nor an effect of age (p = 0.771). As expected, a significant effect of peptide state is seen (free 1618.9 [125.9], total 3664.1 [247.1] pg.mL−1; p < 0.001; Figure 1A).
Figure 1. Concentration of plasma endocrine factors from younger and older participants. (A) Myostatin (free and total), (B) activin A, (C) FLRG, and (D) GDF-11 (all pg.mL−1). Data are presented as grouped dot plots, with horizontal lines indicated mean in panels (A–C), median in panel (D). Note panel (D) is presented on a logarithmic y axis. N = 6 per group, closed circles indicate younger, open squares older participants. *Indicates significant differences as marked.
Plasma activin A was significantly higher in older individuals (younger 215.9 [20.2], older 291.6 [21.0] pg.mL−1; p = 0.026; Figure 1B), as was plasma FLRG (younger 4731.6 [522.5], older 9915.8 [975.1] pg.mL−1; p < 0.001; Figure 1C). No difference in GDF11 was seen between groups (younger 388.4 [274.9–6339.8], older 219.4 [143.2–6694.8]; p = 0.394; Figure 1D).
Cells Grown in Younger Plasma Show a Protective Effect
C2C12 cells were stimulated with standard GM or DM + 5% older or younger plasma for varying lengths of time before proliferation, scratch damage recovery and differentiation capacity was assessed.
No age × time interaction was seen on the proliferation capacity of cells (p = 0.535), nor a main effect of age (p = 0.367). As expected, there was a main effect of time witnessed, with significantly more cells present at 48 h, relative to 24 h (p < 0.001; Figure 2A).
Figure 2. Effect of plasma conditioned media on myoblast proliferation, migration, and myotube size in vitro. (A) Proliferation rate of cells at 24 and 48 h, (B) scratch closure (% of baseline) 4 h post scratch, with (C) showing representative images. Note edges have been highlighted for publication. (D) myotube diameters. Horizontal lines in (A,B) indicate group means. Error bars in (D) indicate standard error. N = 6 per group, closed circles indicate younger, open squares older participants. *Indicates significant differences as marked in (A,B), significant difference between older vs. younger group at marked time-point in (D). Younger/Older conditions represent cell GM (A–C) or DM (D) conditioned with 5% plasma from younger or older individuals, respectively.
When cells were scratched and then allowed to regenerate for 4 h, post scratch size was smaller in the younger relative to the older condition (younger 78.2 [2.2], older 87.2 [2.7]% of baseline; p = 0.0281; Figure 2B, representative images shown in Figure 2C).
Following replacement with conditioned media, myotube cell diameters showed a significant age × time interaction (p = 0.008). Specifically, post hoc testing suggested younger cells were larger at 24 (26.8 [0.5] vs. 23.4 [0.5] pixels; p < 0.05) and 48 h (28.3 [0.3] vs. 22.5 [1.6] pixels; p < 0.05; Figure 2D).
A significant correlation was noted between myotube diameter at 48 h and the plasma concentration of FLRG, independent of participant age (r2 = 0.392, p = 0.030; Figure 3), but not for activin A (r2 = 0.123, p = 0.264). No correlations were noted between scratch closure and FLRG or activin A.
Figure 3. Myotube diameter at 48 h post plasma stimulation as a function of in vivo plasma concentration of FLRG (pg.mL−1). Linear regression indicated, independent of age group. Closed circles indicate younger, open squares older participants.
In line with our hypothesis, here we demonstrate that plasma from older individuals induces both reduced myoblasts migration and a reduced myotube diameter, both relative to those stimulated with younger plasma. The primary endocrine difference reported here between younger and older individuals was in the myostatin and GDF11 inhibitor FLRG, thus it is also of interest to note that circulating FLRG concentration in vivo correlated with the individual myotube diameter response in vitro. However, no difference in GDF11, free myostatin or total myostatin plasma concentration was noted between ages.
By utilising C2C12 myoblasts as a "clean" cell line, independent of ageing effects, the effects we see from plasma conditioning of cellular media and subsequent stimulation of C2C12 cells can be deduced to be plasma-dependent. We developed this model on the observation that mixed results have been reported for an ageing effect on satellite cells and muscle functionality. Indeed, where human primary muscle cells grown in vitro from both younger (23–25 years of age) and older donors (67–82) do not show proliferation, fusion or myotube size differences (Alsharidah et al., 2013), primary culture from older average (78.5 years of age) show reduced myotube fusion (Pietrangelo et al., 2009). Whilst the age of older groups was similar, these samples were taken from hip replacement surgery patients, thus who are likely to have experienced extended disuse prior to biopsy collection, possibly explaining differences in results. These observations lead us to hypothesise that endocrine environment differences may underlie differences in muscle mass and function between ages, and indeed the results that we present here suggest this to be the case. However, purely endocrine factors cannot completely explain loss of muscle mass and function with ageing. Alterations in mitochondrial reactive oxygen species (Desler et al., 2011), altered mechanical stimulation of muscle satellite cells (Boers et al., 2017), alterations in physical activity (Daskalopoulou et al., 2017) and increased low level inflammation (Franceschi and Campisi, 2014) are likely to interact to impair successful ageing and the preservation of muscle mass with age.
The in vivo parallel to the myoblast scratch assay we report here is most likely muscular injury and recovery. In this light, it is interesting to note that older individuals show anabolic resistance to amino acid feeding (Cuthbertson et al., 2005), reduced rates of satellite cells per muscle fibre (Verdijk et al., 2007), and reduced muscle recovery following immobilisation-retraining (Suetta et al., 2009), ultimately resulting in a proposed reduced rate of injury repair and recovery (Fell and Williams, 2008) and increased intermuscular fibrosis and adipose tissue, and a reduced muscle force output (Alnaqeeb et al., 1984; Delmonico et al., 2009). Whilst speculative at this point, the endocrine mechanistic reduction in scratch-recovery in our in vitro older condition may in part reflect the early stages of this process.
Myotube diameter was reduced in our older condition, relative to the younger. Myostatin, GDF11 and activin A have all been demonstrated to inhibit myoblast differentiation in vitro (Souza et al., 2008), whilst follistatin and FLRG can be used to promote fusion, but indirectly via myostatin-dependent myotube inhibition (Hill et al., 2002; Iezzi et al., 2004). With no difference in myostatin or GDF11, we would suggest that the either increased activin A concentration has resulted in reduced fusion in the older group, or the increase in FLRG in the older group is impairing myoblast fusion, as implied by the moderate correlation we report between myotube size and FLRG concentration. This second hypothesis seems initially unlikely, as FRLG has been demonstrated to bind and inhibit myostatin in vivo (Hill et al., 2002), in a similar manner to the closely related follistatin (Amthor et al., 2004), which should be expected to increase myotube size, not decrease as we report here. To the best of our knowledge, the direct effect of FLRG on myoblast proliferation and differentiation has not been directly examined in vitro, however it is interesting to note that FLRG does directly inhibit osteoclast fusion and differentiation (Bartholin et al., 2005).
Whilst not a primary aim of this in vitro experiment, is it noteworthy to report that both total and free myostatin appear to circulate at similar concentrations between younger and older individuals in the limited sample size collected here, despite the later having significantly reduced skeletal muscle mass. Similar results have been reported previously for plasma total myostatin in younger healthy males vs. older sarcopenic males (Ratkevicius et al., 2011), however we further these results with the addition of free myostatin. The same paper reports a trend toward a difference in FLRG concentration between younger and older individuals, whist both Bergen et al. (2015) and our results presented here suggest FLRG is increased in older individuals. The correlative relationship that we report here between circulating FLRG in vivo and myotube diameter in vitro is suggestive of a physiological relationship between the two.
Much recent attention has been placed on ageing and GDF11, with reports that GDF11 decreases with age in mice, and restoration of "younger" levels ameliorates age-related declines in muscular function (Sinha et al., 2014). Further, highly active older individuals show increased levels of GDF11 over inactive older individuals (Elliott et al., 2017). Whilst we report no difference between younger and older individuals here in both myostatin and GDF11, we do acknowledge the study was not powered for this outcome. However, it is of interest to note that the age groups do have similar concentration ranges of these peptides, despite the significant difference in muscle mass seen. It is tempting to infer a sensitivity to the effects of circulating myostatin/GDF11 may be seen in older individuals, helping explain this apparent contradiction, however further work would be needed to state this. Other TGF-β family members have been suggested to contribute to human ageing, and here our results show an increase in activin A, confirming prior findings (Baccarelli et al., 2001).
Whilst not measured here, it should be acknowledged that a large number of endocrine factors are suggested to change with human ageing. These include classic anabolic factors such as growth hormone, insulin and IGF (Ratkevicius et al., 2011; Sellami et al., 2017), as well as the likely catabolic, low level of pro-inflammatory cytokines such as tumour necrosis factor alpha and interleukin 1 beta (Ferrucci et al., 2005; Alvarez-Rodríguez et al., 2012). Further, more recently identified factors such as Klotho act in an endocrine manner in mice and appear to regulate lifespan (Kurosu et al., 2005). A complete picture of endocrinology of aging may need to consider these factors.
In conclusion, here we demonstrate that plasma from older individuals both inhibits myoblast migration and reduces myotube diameter, relative to younger plasma. These effects appear independent of circulating myostatin and GDF11, however may involve activin-family peptide inhibitor FLRG. Whilst our results show part of ageing-related changes in muscle mass may be endocrine in nature, the interaction between this observation and other aspects of ageing need to be further explored. Further work is needed to examine the effect of increased FLRG concentration on muscle function in ageing populations.
IK and BE: conceived this experiment; IK and AvB: conducted data collection and analysis; IK: wrote the first draft of this manuscript. All authors contributed to manuscript revision, read, and approved the submitted version.
The authors acknowledge Ms. Kiu Sum for assistance with sample analysis. This study was financially supported by a Society for Endocrinology (UK) research grant to BE.
Alnaqeeb, M. A., Al Zaid, N. S., and Goldspink, G. (1984). Connective tissue changes and physical properties of developing and ageing skeletal muscle. J. Anat. 139 (Pt 4), 677–689.
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Keywords: ageing, myostatin, GDF11, plasma conditioning, muscle, frailty
Citation: Kalampouka I, van Bekhoven A and Elliott BT (2018) Differing Effects of Younger and Older Human Plasma on C2C12 Myocytes in Vitro. Front. Physiol. 9:152. doi: 10.3389/fphys.2018.00152
Received: 02 December 2017; Accepted: 13 February 2018;
Published: 27 February 2018.
Kevin I. Watt, Baker Heart and Diabetes Institute, Australia
Daniel Lee Clark, The Ohio State University, United States
Richard Jaspers, VU University Amsterdam, Netherlands
Copyright © 2018 Kalampouka, van Bekhoven and Elliott. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Bradley T. Elliott, b.elliott@westminster.ac.uk


This workshop addresses the new changes to Canadian citizenship law. Bill C-24, the Strengthening Canadian Citizenship Act, which was passed in June 2014, contains a variety of changes which will dramatically reshape citizenship in Canada. Some of the provisions already came into effect on August 1, 2014, while other provisions will come into force later (likely by May 2015).
The workshop explains these changes, the steps to follow, and what to include to apply for citizenship. The presenters also explain what to expect after the application is submitted and advise on potential pitfalls, including misrepresentation, cessation, and vacation.
This workshop will also highlight who will be most impacted by these changes and provide practical tips for completing citizenship applications.
The Toronto South Local Immigration Partnership (TSLIP) is a community partnership focused on developing local settlement strategies that coordinate and enhance service delivery to newcomers while promoting innovation and efficient use of resources.


text:
VIDEO: Charges recommended for man caught beating …
VIDEOS: Relive all the festivities of Gasparilla
Project Dynamo rescues more Ukrainians, including babies
by: Niko Clemmons
Posted: Apr 22, 2022 / 10:03 PM EDT
Updated: Apr 22, 2022 / 11:14 PM EDT
TAMPA, Fla. (WFLA) — More than five million Ukrainians have fled their country since the invasion by Russian forces began nearly two months ago.
The Tampa Bay area organization Project DYNAMO continues to evacuate hundreds of people, including newborn babies. It's the latest rescue effort by the organization.
The group has rescued more than 150 people in the last week, including newborn American and British babies, using 8 cars and three buses to complete the mission.
The rescue comes a month after team members finished another dangerous mission to rescue three premature babies from Ukraine.
One of the group's co-founders talked about the dangers then.
"At both hotels, we could hear artillery in the city," Project DYNAMO co-founder Bryan Stern said.
To date, Project Dynamo has rescued more than 550 people from the most war-torn, contested, and Russian occupied areas of Ukraine and taken them to neighboring countries.
PHOTOS: Gasparilla Parade of Pirates


text:
Q: git push never finishes - only gets to Total line Expected Behavior
I edited file1, committed, and did this
$ git push

NOTICE: Only authorized blah blah blah...

Counting objects: 7, done.
Delta compression using up to 4 threads.
Compressing objects: 100% (7/7), done.
Writing objects: 100% (7/7), 676 bytes | 0 bytes/s, done.
Total 7 (delta 4), reused 0 (delta 0)
To ssh://me@server:/opt/git/fooBar.git
   28ad03d..73ae492  master -> master
$

Problem
Then I edited file2 in the same project, committed and did this:
$ git push

NOTICE: Only authorized blah blah blah...

Counting objects: 10, done.
Delta compression using up to 4 threads.
Compressing objects: 100% (8/8), done.
Writing objects: 100% (10/10), 1.42 KiB | 0 bytes/s, done.
Total 10 (delta 4), reused 0 (delta 0)
^C

Notice how I had to CTRL-C (^C) at the end?  That's because it never completed.  This is NOT the expected behavior.
Failed Coping Mechanisms
So I:
cd ..
mv fooBar/ fooBar_bak/
git clone ssh://me@server:/opt/git/fooBar.git
cd fooBar

Edit some other text file (just added a blank line), pushed, and it worked.  This means I did a successful push from/to the same local/remote repositories before and after the failed push.
But edit file2 it didn't like before?  The push never completes.  Same thing with file3 which it doesn't like either.  I don't see anything special about these files.
Well, file2 and file3 are UTF-8 and file1 is ASCII, but that shouldn't matter.  Should it?
I tried all the solutions at git push hangs after Total line but none worked for me.
I didn't really understand the output of strace -efile -f git push but nothing jumped out at me.
I waited.  But this is not a big repo or a big file.
I'm using Ubuntu 16.04 with git version 2.7.4 on my machine and git version 1.7.1 on the CentOS server.  I don't have msysgit, git-for-windows, or cygwin installed.
git push -u origin master didn't work either.
git config http.postBuffer 524288000 didn't make any difference.
GIT_CURL_VERBOSE=1 and GIT_TRACE=1 had no effect.
git config --global core.askpass "git-gui --askpass" didn't do anything, probably because I don't have git-gui installed.
@RonanDejhero's suggestion here: https://stackoverflow.com/a/21032615/1128668 was what allowed me to see that some files can be changed, committed and pushed and some cannot.  I tried copying the changed files, changing the files manually, and making slightly different changes: no good.
On the server I did sudo chown -R me.git-users /opt/git/ but that didn't make a difference either.  When I've had problems like this in the past, that seemed to help.
I did a git gc in my local repo and on the server in /opt/git/fooBar.git.  No joy.
Update
I deleted my local repo, cloned, edited file2 (which git didn't like being edited before) by only adding a blank line.  Committed, pushed, it worked!  Then I commented out a dozen lines of unused code, committed pushed, and it worked!  Could I have introduced a character or something that git didn't like?

A: I just had this exact issue but the cause was different. The origin using
git remote -v

result: origin  pi@raspberrypi:/home/git/...git
I had a user that didn't have write access on my git repo.
The fix was to remove and re-add origin with the correct user
git remote rm origin
git remote add origin git@raspberrypi:/home/git/...git

A: It was my router?!?!?!
The biggest clue was that I tried to SFTP one of the troublesome files up to the server and it failed.  I had installed a new Netgear AC1750 Smart WiFi Router Model R6400 yesterday.  To fix my problem, I simply unplugged the Netgear router and plugged in my old Linksys WRT120N.  Everything worked.
Weird.  You'd think if I'm using a VPN from my desktop and run SSH inside of that, the router wouldn't be able to tell what kind of packet it's sending.  Or that if it goofed up packets that would break everything.  I don't get it.
I told Netgear about my problem and indicated that I might return the router and get something I could install open-source firmware on.  Their response was along the lines of "maybe you should do that" so I did.  Happily running OpenWRT on a Buffalo router now with everything working.

A: I had this behaviour for a different reason, hopefully this answer helps someone else :)
I have a directory on my local git server at home at /srv/git for all my git repos, and user named git-user who owns all the files. I see URLs like ssh://git-user@server/repo to clone the repos and make changes, guaranteeing that git-user owns all the files.
I've been switchng from SVN to GIT for each of my repos. My flow for making a new repo from the latest files of an SVN repo (I don't care about the history, they're just personal projects) is as follows:
ssh <git server>
su -
su - git-user
git init --bare newgitrepo.git
exit

# Back to my desktop
cd git
git clone ssh://git-user@<git server>/srv/git/newgitrepo.git
cd newgitrepo
# Set up initial files - my local Workspace dir is where Eclipse has
# SVN projects already checked out
cp -r ../Workspace/<svn repo>/* .
cp ../Workspace/<svn repo>/<hidden dot files/dirs, except for .svn> .
# Create .gitignore files for stuff like compiled code directory
git add <everything but .git dir>
git commit -m 'Initial Import'
git push origin master

I had a case of one repo that I just couldn't push anything without getting this irritating behaviour of a total line and no further response. I had missed the 3rd command on the server (su - git-user), so I had created my bare repo as root, causing all files to be owned by root. The solution of course was just delete the bare repo on the server and re-create them as git-user.
This lines up with what GlenPeterson said about this being a server problem, and not a client problem.

A: In my case, updating from
git version 2.32.1 (Apple Git-133)
to
git version 2.37.1
helped.
On Mac, it can easily be done with one line: brew install git

A: I had this problem while pushing to a git remote of gigalixir. The problem was that their CLI failed to save the password necessary for pushing to my ~/.netrc file. So, if you push to a git remote over https, please check that your ~/.netrc file contains something like this:
machine api.the_service.com
        login my@email.com
        password some_password
machine git.the_service.com
        login my@email.com
        password some_password (same as above)

redpajama_set_name: RedPajamaStackExchange


Shuttle XPC, the world's famous Mini PC, launches a brand new SFF to create a volume of only 0.5-liter super mini PC called XPC nano. This series has elegant and stunning outlook to make it easy fit anywhere at home or office use. XPC nano is introducing NC01U series, which provides different CPU options: Intel® Celeron®, and Core™ i3/i5/i7 as well as supports DDR3L-1600 low-voltage memory. NC01U3/U5/U7 equipped with Core ™ i3/i5/i7 can support 4K / Ultra HD video playback, providing stunning visual experience. The series features diverse I/O ports for great connectivity, including USB2.0, USB3.0, RS232, SATA 6G, M.2, and Intel® Gigabit LAN as well as 802.11 ac/b/g/n high-speed networks. The NC01U series has one unique extension design to provide users a variety of choices by adding a docking station. Whether for seamless desktop performance to run an efficient office or to play 4K video (except for NC01U) for multimedia, XPC nano is your perfect choice.
There are a variety of CPU options of XPC nano, including Celeron® 3205U and Core™ i3/i5/i7 processors. The series supports DDR3L 1600 low-voltage memory with maximum capacity up to 16GB (8GBx2).
The NC01U series with a variety of CPU options, equipped with Intel® Celeron® processor can support 1080P/ Full HD; equipped with Core™ i3/i5/i7 is able to support 4K/Ultra HD* video playback.
*Support 4K dual-channel memory is required.
This new 0.5-liter XPC nano supports SATA 6G storage interface for 2.5" HDD/SSD (7mm ultra slim) and built-in one M.2 slot for high-speed M.2 SSD, proving the best data transfer efficiency.
The XPC nano has built-in Intel®10/100/1000 Mbps LAN interface and supports Wake On LAN function. Equipped with the newest 802.11 b/g/n/ac dual band WiFi and Bluetooth 4.0 combo, can enhance data transfer speed.
Use BIOS to set up the Wake ON LAN function so that users can turn on the NC01U series – from the sleep mode, rest mode, or when powered off – via the internet.
XPC nano provides HDMI and mini DisplayPort video outputs to support dual-screen display. Use an adaptor to cover HDMI/mini DisplayPort to DVI or analog video interface (D-sub) for diversified signal selection.
The NC01U series uses high quality materials to meet the environmental operation demands of commercial applications. Its rugged design allows for stable operation over long periods of time – even in 50⁰C environments*.
XPC nano has all the functions you need from ultra SFF (small form factor). Equipped with multiple I/O ports of HDMI, mini DisplayPort, RS232, USB2.0 and USB3.0 as well as SD card reader, both USB3.0 ports also provides 2A current for fast charge.
Designed to fit seamlessly into your lifestyle, the XPC nano is capable of mounting to the rear of LCD monitor or a TV. This space saving capability makes it easy to blends into anywhere, including home, any offices, and commercial environments.


text:
Research Catalyst Award Winner to Study How Megafires Will Impact Forest Food Web
Research Catalyst Award Winner to Study How Megafires Will Impact Forest Food Web UC Davis Ecologist Rahel Sollmann Selected for UC President's Award
By Kat Kerlin on December 2, 2016 in University
UC Davis professor Rahel Sollmann doing fieldwork in Brazil. Sollmann is a 2017 recipient of UC's Research Catalyst Awards.
As the severity of wildfires increases each year across the West, how megafires affect forest ecology is an increasingly urgent question.
Now, UC Davis Assistant Professor will be able to explore that question more broadly. She was one of three recipients of the 2017 Research Catalyst Awards by Stuntverkoop President Janet Napolitano.
Sollmann is a quantitative ecologist in the UC Davis Department of Wildlife, Fish and Conservation Biology. With the $271,000 in funding provided by the reward, she will work with UC Berkeley Associate Professor Justin Brashares and UC Santa Barbara Assistant Professor Hillary Young, as well as other researchers at UC Davis and the U.S. Forest Service to analyze how megafires disrupt the forest ecosystem and food web and pollination networks. The research will focus on the King Fire, which burned more than 97,000 acres in El Dorado County in 2014.
"Forests are really at the heart of California economy, culture and its ecological heritage," Sollmann said. "The Forest Service has identified these large fires as the major threat to the integrity of California forests. We really hope to understand how large fires change forest pollination and food networks."
The three awards, totaling more than $2 million, involve faculty and students from across the university with lead campuses at UC Santa Barbara, UC Santa Cruz and UC Davis.
The other projects include research led by UC Santa Barbara aimed at improving teacher training and student outcomes in California's K-12 schools ($1.5 million); and a UC Santa Cruz project ($278,000) to enhance knowledge of human health through paleogenomic research.
Napolitano launched the President's Research Catalyst Awards in 2014 to foster multicampus, interdisciplinary research in areas of strategic importance to California and the world. Since its inception, the program has provided nearly $10 million for research focused on climate change, cultural preservation, drought, basic science and more, and afforded research opportunities for 50 UC faculty, nearly five dozen graduate students and 20 undergraduates.
Recipients were selected following a highly competitive review process. A panel of experts evaluated applications based on scholarly merit and likely impact. President Napolitano selected the winners from among the top-ranked proposals representing all areas of university scholarship.
Funding for the Catalyst Awards comes from the UC president's endowment funds, which support systemwide initiatives and projects.
can be found online.
Kat Kerlin, UC Davis News and Media Relations, 530-752-7704, [email protected]
Sollmann
Megafires
University Environment
Automated Shuttle Comes to UC Davis West Village
UC Davis Takes 10 Electric Cars on Extended Test Drive
протеина
купить таблетки для потенции в украине


text:
UK Prime Minister: Post-Brexit Transition Could Be Extended
British Prime Minister Theresa May said Thursday she is considering a European Union proposal that would keep Britain bound to the bloc's rules for more than two years after it leaves, and idea that angers her pro-Brexit critics in the U.K.
Jill Lawless, Associated Press
BRUSSELS (AP) — British Prime Minister Theresa May said Thursday she is considering a European Union proposal that would keep Britain bound to the bloc's rules for more than two years after it leaves, and idea that angers her pro-Brexit critics in the U.K.
At present the two sides say Britain will remain inside the EU single market, and subject to the bloc's regulations, from the day it leaves on March 29 until December 2020, to give time for new trade relations to be set up.
But with divorce talks stuck, the bloc has suggested extending that period, to give more time to strike a trade deal that ensures the border between Northern Ireland and the Republic of Ireland remains friction-free — the main sticking point to a Brexit deal.
May said the U.K. was considering extending the transition period by "a matter of months." But she said she didn't believe the extension would be needed.
"We are working to ensure that we have that future relationship in place by the end of December 2020," May said as she arrived at EU headquarters in Brussels on Thursday for meetings on migration, security and other issues.
The extension idea has angered pro-Brexit U.K. politicians, who see it as an attempt to bind Britain to the bloc indefinitely.
In an open letter to May published on Thursday, leading Brexiteers accused the EU of "bullying" and said the border issue was being used as "a trap" by the bloc.
The letter signed by former British Foreign Secretary Boris Johnson, ex-Brexit Secretary David Davis and other pro-Brexit Conservatives warned May not to "engage in a show of resistance and a choreographed argument followed by surrender" to the EU.
Divorce talks between Britain and the bloc have stalled on the issue of the Irish border, which will be the U.K's only land frontier with the EU after Brexit. Both sides agree there must be no hard border, which could disrupt businesses and residents on both sides and undermine Northern Ireland's peace process. But each has rejected the other side's solution.
This week's summit, which had been billed as a make-or-break moment, turned into a chance for both sides to give themselves more time — perhaps until the end of the year — to break the logjam.
May urged both parties to show "courage, trust and leadership," but came to Brussels without the concrete new proposals the EU has asked for. Chief EU negotiator Michel Barnier said that "we need much time, much more time, and we continue to work in the next weeks."
The lack of progress means a special EU summit on Brexit that had been penciled in for next month to finalize a deal has been scrapped, though EU leaders said they would assess the situation in the coming weeks.
The next official EU summit is scheduled for December, just over three and a half months before Britain ceases to be an EU member. Any deal that is struck will have to be approved by the British and European Parliaments.
Conservative lawmaker Nick Boles said there was a growing worry among many U.K. legislators that Britain and the EU were "trying to run out the clock" in order to stymie opposition to their plans.
"They are trying to leave this so late that they can credibly say there is no alternative but a 'no-deal' Brexit, and most people agree that would be chaos," Boles told the BBC.


Skanska is to construct homes and offices in central Stockholm. The project, worth approximately SEK 450 M, will be developed and constructed by Skanska. The 150 units will be sold as tenant-owner apartments in a newly formed tenant-owners association.


Helm Hill was the venue for this week-ends event. This is the domain of the Helm Hill Runners - their summer training ground. It has everything including some short sharp climbs and plenty of running. If you come here, prepare for battle!
This weekend saw a huge number of races take place across the country and numbers were inevitably down as a result. However, the competition was of an equally high standard. The senior race saw Helm's Alastair Dunn take on the rest of the 48 seniors to keep him on track for the 2009 BOFRA championship. Conditions were ideal underfoot and the course was well cut out, Alistair put in a tremendous run coming home 1st BOFRA counter in 2nd with only one athlete, Thomas Cornthwaite who runs for Blackburn, in front of him. These guys were well clear of Mark Croasdale as first V40 with another V40 Paul Dugdale in 4th. Billy Proctor had a tremendous run finishing in 5th and 1st V50 just 4 seconds ahead of Brendon Taylor. First u'23 man was James Mountain in 11th.
With some of the Big Gun's missing in the ladies race Helm's Kath Aubrey put in a great run to come home 1st lady, with Val Done in 2nd and Lynn Whittaker taking 3rd prize.
The u17's race saw Richmond runner Marc Scott come back well clear of last year's champion Tom Sessford in what is a very competitive age group. Brothers Ryan and Luke Croasdale closely followed Tom to come back 3rd and 4th just 8 seconds parting them. Helm's Jos Addison finished 5th with Helm's Mel Hyder showing her class coming back in 6th and first girl. Mel is one of Helm Hill Runners Superstars and she's in great form at the moment. Second girl, Helm's Lauren Munro Bennett is new to this age group so this was a good effort. Third prize went to Beth Dutson.
In the under 14's Helm's James Knox has dominated this age group, if he turns up the rest usually end up getting hauled round by him. This is the closest I've seen anyone come to him in this age group and he held on to the lead coming back just 4 seconds clear of Tamworth AC's Kristian Edwards with James Hall the same margin back in 3rd. These were well clear of David Goad and Phil Done who came home in 4th and 5th. Helm's Megan Stuart finished as 1st girl with Wharfedale's Nicole Narey closely in pursuit, whilst 3rd place went to Zara Knappy. 19 u14's ran on the day which is quite a good turnout.
In a well supported under 12's race Neil Orr who runs for Border Harriers carried on his winning ways, he certainly knows where the winning line is, coming back well in front of 2nd place Luke Canning with Skipton's Joe Stapleton in 3rd. Helm had the next 3 finishers with Ben Kent, Chris Richards and Oliver Willink finishing in that order. Wharfedale's Ellamae Gibson put another great performance in coming home 1st girl with Keighley's Holly Carter and Settle's Martha Pickles taking 2nd and 3rd prizes, Zoe Baistow was just 3 seconds adrift and was 4th girl.
Moving on to the apprentices, the under 9's - these athletes have everything in front of them they are the Superstars of the future. Helm's James Marsh halted Robbie Johnstone's winning spree coming back 4 seconds ahead in what was a hard fought dual. Helm's Joe Kent came 3rd just 2 seconds in front of the 4th runner overall first girl Shona Hastings who just pipped Helm's Tom Willink who came back 5th. Keighley's Emily Cox and Tegan Knappy took 2nd and 3rd prizes in the under 9's girls.
Taking everything into consideration on the day, when you come into the realm of the Helm Hill Runners prepare yourself well. Nothing comes easy, it has to be earned the hard way, they give nothing away.
Lastly a lot of work goes into organising races, so a big thank you to the people who cut out the course and flagged it, and to our people who take entries, times and places. Without these it would just be a training run. And a big thank you to all who supported our race either running or spectating.


i'm a sucker for a good cookie. aren't you? peanut butter, chocolate chip, oatmeal. i'll take them all. and lucky for me, this cookie has all three rolled into one!
i made a batch of these the day before i left on a four hour drive with a carload of women. 55, 50, 29, and 19 year old women to be exact. surprisingly, no one ate a cookie until midafternoon. when it was pouring rain and we stopped at a gas station. three of us waited in the car while one ran in. it was that exact moment when my mother said "hmm, ok, i'll take one of those cookies now." perfect timing. the three of us devoured eight in the next two minutes.
note: the dough must sit in the refrigerator overnight, so plan ahead! and jessica's rendition of the recipe is perfect, so why change it?
2013.02.04 this is Barbara Bakes' pick for the secret recipe club!
You were my SRC blog this month and I made these delicious cookies for a Valentine's treat. They were a big hit. Thanks for sharing!


We've been experimenting with hilling discs in our potato patch. Last week we tried it, but the ground was wet and the weeds matted, so the discs were just lifting sheets and then dropping them back in place, slightly disturbed. After a few (somewhat) dry days and some light rototilling along the rows, we used the discs again.
Two rows, 140 feet long. Not enough to sustain us for a year, but we're getting closer to our goal of providing the majority of our foodstuffs. The row on the right is sparse in spots but most of the plants look fine. Although thus far we've only had minor bug problems, it's time to start vigilant Potato Beetle patrols.
It took several adjustments to work out the optimal ground speed and cutting width, but with practice we got good results. It got me thinking about building a two-row toolbar to run two sets of discs so I could gather hilling material from a wider swath instead of making multiple trips up and down the row. The effort and cost wouldn't be tremendous, but it probably isn't worth it for as small a potato crop as we grow. Still, it is fun to dream up garden implements.


text:
Q: How to obtain aws account ID from bash script So, I would like to obtain my aws account id through a bash script.  I know that when I run aws sts get-caller-identity, it returns a json (similar to)
{
    "UserId": "JALDKKEABANMFSL:john.doe",
    "Account": "10299301874829",
    "Arn": "arn:aws:sts::10299301874829:assumed-role/admin/john.doe"
}

is there a way for my to either just get the "Account" value from the returned json?  I've tried saving the response into a .json file and using jq, but that has been throwing errors.  Any ideas?  Thanks!!

A: The AWS CLI uses JMESPath to access elements of the output:
aws sts get-caller-identity --query 'Account' --output text

This will extract the Account value from the returned dictionary and output it in plain text.
JMESPath is extremely powerful. I highly recommend looking at the JMESPath Tutorial.


text:
Pero garuparia är en fjärilsart som beskrevs av Felder 1874. Pero garuparia ingår i släktet Pero och familjen mätare. Inga underarter finns listade i Catalogue of Life.

Källor

Mätare
garuparia


We don't ask this question enough.
Has too many options associated to it.
Invites too much feedback from those involved.
Turns into a much longer discussion than we are willing to invest time in.
Or simply put – "It's too hard to ask" and that much harder to receive feedback on.
When we say "What Happened?" – we are opening ourselves up to feedback to everyone – those directly and indirectly involved – it can be overwhelmed if we don't have a process in place to handle, consume and manage it.
But that's a process issue and is easily dealt with.
The bigger question is whether you really want to know what happened and implement change to fix it?


text:
Cold Case: 1989 East El Paso bludgeoning death remains a mystery
by: Andra Litton
Posted: Jan 9, 2021 / 11:02 AM MST / Updated: Jan 10, 2021 / 09:20 AM MST
EL PASO, Texas (KTSM) — It's been nearly 32 years since anyone saw Stephen Michael Hicks alive. Police haven't given up hope that his killers can be brought to justice.
Hicks' body was found near his Ford F-150 pickup truck in an isolated industrial area known as "Cherry Hill" in the early morning hours of Sunday, April 9, 1989. According to police, he'd been bludgeoned to death.
Police say, Hicks, who was only 23 years old at the time, was out with his sister at Bowl El Paso in the 11100 block of Pellicano earlier in the evening and briefly ran into his house in the 1600 block of Gene Torres Drive before leaving again around midnight.
Around 1 a.m., a truck driver reported seeing Hick's white Ford F-150 at a truck stop about a mile from where his body was eventually located. At 2 a.m., a passerby spotted Hick's body next to his truck in the 1300 block of Don Haskins and immediately contacted police.
Back in the late 80s, the area was known as 'Cherry Hill,' a remote industrial area frequented for bonfires, drinking, and other illicit activity. Investigators believe Hicks was facing someone and was attacked from behind. They suspect that more than one person was involved in his beating death. A weapon was not left at the scene.
Anyone having information on the murder of Stephen Michael Hicks should call Crime Stoppers of El Paso immediately at 566-8477(TIPS) or online at www.crimestoppersofelpaso.org. You will remain anonymous, and if your tip leads to an arrest, you may qualify for a cash reward.
KTSM Video Center
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Fort Bliss soldier accused of sexual assault identified
Rapid Testing
Food Truck Fridays - Taconet, a new local food shop that opened during the pandemic
Food Truck Fridays - Taconeta
Smoothie assault arrest
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Woman in viral video throwing smoothie at store employee arrested
EL PASO, Texas (KTSM) -- A woman who was seen on video throwing a drink at a smoothie store employee in early January has been arrested.
Pamela Underwood, 45, was arrested on Thursday and charged with assault causing bodily injury, according to the El Paso Police Department.
No threat to El Paso identified so far as FBI remains on alert ahead of Inauguration Day; Congresswoman Escobar asks community to help ID rioters
by Erin Coulehan / Jan 15, 2021
EL PASO, Texas (KTSM) -- The FBI El Paso Division is joining national efforts to combat public safety threats in the days leading up to Inauguration Day.
On Friday, Special Agent in Charge Luis Quesada released a statement on the safety measures being taken locally following last week's violent attack inside the U.S. Capitol.
One person critically injured in officer-involved shooting in South Central El Paso
by KTSM Staff, Shelby Kapp / Jan 15, 2021
EL PASO, Texas (KTSM) -- One person was critically injured in an officer-involved shooting on Friday morning. This is the second officer-involved shooting that happened Friday morning, this time in South Central El Paso.
According to the El Paso Police Department, one person was transported to a local hospital with critical, life-threatening injuries after the shooting, which happened at 6000 Tejas Drive.
More crime
El Paso News / 7 hours ago
Crime / 13 hours ago
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EPPD: Man threatening motorists with rifle involved in shooting with officers


text:
Asunder, Sweet and Other Distress (2015)
Reviewer RENALDO69
If I had to choose one word to best describe Godspeed You! Black Emperor, it'd be cinematic. Just ask director Danny Boyle. Using them in 28 Days Later was an appropriate way to bring out the sense of dread and impending apocalypse, which I admit got me to open my mind up to more genres like black metal, post-rock and heavy instrumentals. I found a deeper, more meaningful appreciation for bands like Deafheaven and Black Clouds and I realize (after taking in this album) that a lot of this can be chalked up to GY!BE's portfolio. Asunder, Sweet and Other Distress is another edition of jarring post-rock, experimental haze and once more, a call to arms for a band that really takes the concept of music and theatrics one step further.
This record clocks in at 40 minutes and the imagery they paint is as vivid as can be. "Let your imagination run wild" is my motto with these Canadians. "Peasantry, or 'Light! Inside of Light!" runs for 10 minutes and rattles off like an army marching to war. It's Armageddon to the beat of Black Sabbath. Immediately, there's a buzzy sense of urgency amid their spacey post-rock landscape. They hint at much more vast guitar-work throughout this song but this isn't explored or fully realized until the closer, leaving the mid-sections as fillers. The clashing riffs on this song help to build it as something that'll never linger as an afterthought but as a prominent war-cry. However, the second half of this track flows more melodically with tinges of violins. The string work contrasting with the hard-edged and scraping riffs compensate well for the lack of aggressiveness which I felt the kit needed to produce to complete this track. Overall, I envisioned an army a la Lord of the Rings on the field in a grimy throwdown but as the track concludes, swords stop clashing and the heroes emerge victors. Starting at darkness, then the light emerges. Like I said, I love how GY!BE leave so much to interpretation.
"Lambs' Breath" is another 10 minute jam but one that feels ominous. Demonic, even. It commences with a distorted guitar setting and as it announces itself, the screechy feel of the track gnaws at you. It then slowly tapers off, drifting into a soft hum. What did this paint? To me, an exorcism. But an appreciated breather from the opener. At this juncture, you're probably going to be wondering if they get any heavier. The third track, "Asunder, Sweet" doesn't help alleviate this concern. It flops as a six minute interlude or misguided excerpt. It's so sour that it throws back to the precursor track and leaves you wondering - 'How did these two jams take so long to start and kick in?'. The latter's especially frustrating as you're waiting for a payoff that never comes. However, if this song followed the opener, things might have felt differently. This quibble in arrangement doesn't stick for too long though.
Why? Because the closer in the 13 minute drama "Piss Crowns Are Trebled" smashes home as the crowning statement of the band. It defines the album with its string/violin-esque intro that calmly lulls into harmony. It's entrancing and has you giddy as you wait for an anthem. What ensues is a clever percussion-style that nods once more to a marching beat (a la the opener) and if you're a geek like me, you're picturing a gothic Game of Thrones moment with dragons in the sky. But, the climate shifts at the 5:00 mark or so with a huge symphonic swell. One that's done with such beauty and more so, a transition that's sweetly organic. This song's sectioned into thirds though and the final part's the real kick. Things get progressively aggressive and segue into a symphonic power-metal finale. It's a mighty and downright majestic crescendo to remind you just what this band sparks in you. Asunder, Sweet and Other Distress reinforces all the strengths of the band and with these four tracks, Godspeed You! Black Emperor continue to be an experience as opposed to just another arbitrary music product or pretentious concept. Trust me, you'll need some popcorn for this shit!
VHS: Vultures & Hungry Spirits [EP]
The Meatmen / Against the Grain: Live in Philadelphia


Thanks to the huffingduster You Tube channel here's a video of Nobunny performing a solo set at a house show in Berkley, CA on July 12, 2008. Damn, I'd love to see a Nobunny house show! Shannon and the Clams and The Ears were also on the bill.
DAYEMMMM! The Burger Boogaloo (July 5-6 in Oakland,CA) features an epic line-up this year: Ronnie Spector, Milk 'N' Cookies, Nobunny, Shannon & The Clams, Juan Wauters, Thee Oh Sees, White Fang, The Muffs, OFF!, The Reigning Sound, Personal & The Pizza, Pookie & The Poodlez, Summer Twins, La Sera, and more!
Music Video: Shannon and the Clams – "Rat House"/ Show Tonight @ Great Scott!
Check out the new video for Shannon and the Clams' "Rat House" and then go see them live tonight at the Great Scott with our top dawgs, Atlantic Thrills and Fagettes! Remaining tour dates listed below.
Fagettes and Atlantic Thrills Opening For Shannon & The Clams!
Our two favorite New England based rock 'n' roll bands opening for one of the best national rock 'n' roll bands today – this is the bill we have been dreaming for!


Large oval silver belt buckle with polished silver berry trim. The shining silver plate is fully engraved, featuring a detailed silver cross in the center and a polished scroll across the bottom engraved with the message "For whoever calls on me shall be saved", inspired by John 2:32. A gold Christian cowboy figure is applied to the center of the belt buckle. Standard 1.5" belt swivel.


"We plan to welcome 44 ships to Gothenburg this year, half of them to the new America Cruise Terminal. In total, more than 60,000 cruise passengers will travel to Gothenburg in 2018," said Liselott Bjälving, cruise operations manager for the Port of Gothenburg.
Among the big customers this year are Costa Crociere and Viking Cruises.
"And with the new America Cruise Terminal we hope to attract the slightly smaller cruise ships, those who often target an exclusive customer group, and who want to find central terminal locations in the city," said Bjälving.
Traffic is moving up year-over-year, with 2019 looking even better with 62 ships booked and 100,000 guests expected.
The new terminal is helping drive business up, as Gothenburg now offers two cruise terminals and two cruise quays, able to take ships of any size. There are no limits on ship height or beam, and tug boat assistance is not required.
The port can also offer LNG bunkering by barge, and has recently invested in more capacity for ships to discharge black and grey water.
"Gothenburg offers the vibrancy of a city, an archipelago that is unparalleled anywhere in the world and the largest port in the Nordic Region – all in one. It's easily accessible location on the west coast of Sweden and the rapid approach make it an attractive port of call for cruise ships," noted Bjälving.


Stunning 4 bed/2.5 bath home, situated on a private cul-de-sac, built in 2017, is better than new! This URBAN CHIC architectural beauty boasts a huge OPEN kitchen, white lacquered cabinets, solid QUARTZ countertops, huge island with seating for 4, S/S appliances including a GAS range, and a walk in pantry. This 2 story home, with Master Bedroom & Bathroom on the 1st floor, has an all tile shower with a built in seat. The 4th Bedroom, also on the 1st fl., is situated in the front of the home, has a BARN door and a closet, and is pictured as an office. Upstairs you'll find 2 nice size bedrooms, a full bath, and a small loft. At Yale Court, you're just minutes to PCH, SR51, Loop 202, I-10, amazing restaurants , cafes, and shopping! Cut to the chase, see this home 1st.


text:
Third Girl (A Terceira Moça, no Brasil / Poirot e a terceira inquilina ou A Suspeita, em Portugal) é um romance policial de Agatha Christie, publicado em 1966.

É um caso investigado pelo detetive belga Hercule Poirot e também conta com a participação da escritora Ariadne Oliver.

Enredo
Uma jovem chamada Norma Restarick procura Poirot para dizer-lhe que pensa que cometeu um homicídio. No entanto, ela vai embora logo em seguida, porque acha que ele é velho demais para ajudá-la.

Poirot tenta descobrir quem é a garota e, uma de suas primeiras descobertas, é que ela conhece a sua velha amiga Ariadne Oliver, autora de romances policiais. Ao refazer os passos da garota, Ariadne descobre que ela divide um apartamento em Londres com duas outras jovens(Claudia e Frances), por isso ser chamada de "terceira moça". Além disso, tem um pai e uma madrasta com os quais não tem um bom relacionamento, e um namorado suspeito, chamado David Baker.

Enquanto Poirot e Ariadne investigam, vêm à tona vários fatos sobre os amigos e familiares da moça, tramas paralelas, ligações, e mais mistérios. O detetive acha que todos os fatos são peças de um quebra-cabeça, porém não consegue formar o quadro completo e extrair a verdade.

Afinal de contas, quem morreu? E como? Foi mesmo ela quem matou? Se não, por que acredita que sim? O que há por trás de tudo isso?

Somente Hercule Poirot pode descobrir.

Livros de Agatha Christie
Livros de 1966
Romances policiais


On International Firefighters' Day, we celebrated the launch of Enchantress' first launch of Enchantress Magazine issue in honor of FDNY firefighters.
Issue No. I: FIRE features four women FDNY firefighters on the cover.


Do YOU know how to save a life? That's the question asked by a local organisation - CSALS - as they, in partnership with Bolsover District Council, launched a world record attempt for 'The World's Biggest First Aid Lesson' on Friday 5th Feb.
The record is currently held by Emirates Airlines, with 1,600 in attendance, but the effort on May 20th at the Proact Stadium at 10am, hopes to attract over 3,000 participants of all ages - every one of whom will leave with vital life saving skills.
Dignitaries (above), including The Mayor and Mayoress of Chesterfield and all three local MP's - Toby Perkins, Natascha Engel and Dennis Skinner - heard Cheryle Berry from CSALS talk of how the not-for-profit organisation aspired to have "a lifesaver on every street" and regularly give free first aid sessions for anyone in order to achieve that.
After the launch, Cheryle spoke with The Chesterfield Post and told us just how vital these skills were, "Our group was set up about five years ago and is about saving lives by teaching first aid skills. Over the last five years we have taught 27,000 people - all for free - and this event today is to raise the profile of the importance of life saving skills.
"We are approaching schools and groups to take part. Everyone will leave being a lifesaver and there can't be anything more precious than that!
"Here today are two men whose lives were saved and they are the first to say thank you to those who did it on their day.
"Anyone can turn up on the day - be ready to start at 10am, you will be on the pitch. If anyone would like to have sessions we have a website or contact us by telephone - we will go where you want us to be!
"We are very proud of the support we've had from Bolsover District Council, The Rotary Club and Inner Wheel, British Heart Foundation and other organisations like East Midlands Ambulance Service and CRY (Cardiac Risk in the Young), If we work together we can have a life saver on every street. The more we pull together we can do this!"
One person at the launch owes his life to the skills of other ordinary people around him and is backing the event all the way.
Melvin Kenyon is an Oldham fan who collapsed at the Proact when the Latics took on Chesterfield in November 2015. Several fans present, including an off duty paramedic (below right), performed CPR on the 58 year old, until medical help arrived.
Having made a near full recovery, Melvin (above left) cannot praise those who helped on the day highly enough and was keen to encourage others to take up those skills too, "I am very proud to be at the launch and very grateful to the skills of people who were able to bring me round when I collapsed. I have no memory of it at all - it is good to be here today to try and get memories back but I have no recollection of it at all! It was a traumatic event and it's not surprising to have no memory of it.
"These kinds of events can happen to anyone at any time and the more people that know about life saving skills - for example during this world record attempt - the better it's going to be. One life saved is one life saved.
"I'm feeling a lot better - I'm hoping that Oldham will stay up and that I can come here to the Proact again with them next year!
"The first people to give me CPR were Oldham supporters - one happened to be a trained paramedic - and they started work on me and as a result, I'm here today. I was in the right place at the right time, and so were they!
"I would like to think that every football ground in the country had to have, as a legal requirement, a defibrillator and trained staff. It isn't, I believe, a legal requirement, and if it isn't - then it should be!"
MP's Toby Perkins and Natascha Engel pledged their support for the attempt too with Natascha (MP for NE Derbyshire) saying, "It's something they have been working on for a very long time and this is just the planning stage and it's going to be massive success. This is being organised by volunteers and it's gong to put the area on the map.
"It's also a ripple in the water and this is about teaching life saving skills. People can go and pass those skills onto their families - they have the confidence then to pass their skills on and that's critical.
"Community initiatives like this are brilliant - these people are the heart and soul of communities. They're the real glue that holds communities together!
"Anyone who already has life saving skills please come along and help teach others on the day! Let's break that world record!"
Toby Perkins (MP for Chesterfield) added, "It's crucial - it will be real asset to the community if we have 3,000 more lifesavers. Someone nearly died here at the football ground recently and he survived because there was a lifesaver here at the ground. You never know the circumstances where it may be needed, and to know that you have the skills and you can save a life is important.
"We have a Bill going through Parliament to try and have life saving skills brought onto the school curriculum."
Anyone interested in attending the event itself on May 20th - or in organising a session at a group, can contact CSALS on csals@btinternet.com or call 07876 756 356.


Nicole has a professional work ethic coupled with a high level of integrity. With a "Will to Win" attitude towards business, she achieves through determination and hard work, the best possible results for her clients.
Bernie's family of three were all raised in Bayswater and this has given him a strong affinity for the area and understanding of the local real estate market. Whether buying or selling Bernie has the skills to help you achieve your real estate goals.
Belmont house values increased by 12% over the last year to $1,184,250, Bayswater values increased 11% to $1,370,850 and Narrow Neck was up 5% to $1,506,950.
Most expensive sale: a cool $10m in May 2016, for a 1,000m+ home on a 2,000m+ seafront section in Winscome St, Belmont. To find out who sold it and the best agents to sell your home, go to our Agent Selector Service.
The average time it takes to sell a property in the general Belmont area is currently 34 days.
The agencies of Takapuna and Devonport tend to service this area and acquire the vast majority of listings. The agents are competitive and it's worth asking them to demonstrate that in their proposals.
Private sales are not uncommon, especially by enthusiastic young couples talking up the area as the next Takapuna and keen on contributing as much as possible to the equity of their next home, but the large agency groups and North Shore specialist agency brands tend to dominate.


Tomás B. Ramos is Professor of Strategic Environmental Assessment and Planning at NOVA University Lisbon, School of Science and Technology, and senior researcher at CENSE, Center for Environmental and Sustainability Research. He holds a PhD on Environmental Engineering and his research activity is focused on sustainability assessment and indicators, including impact assessment, stakeholder engagement, organisational performance management and reporting. He is Associate Editor of the Journal of Cleaner Production and member of the Editorial Board of the journals Sustainable Development, Business Strategy and Development, Heliyon, and Journal of Environmental Assessment Policy and Management. He is also member of the Board of Directors of the International Sustainable Development Research Society (ISDRS).