Brevibacillus choshinensis is a Gram-positive bacterium well-suited for heterologous protein expression. The Brevibacillus Expression System II generates secreted target proteins efficiently (Takagi et al. 1989) and is ideal for eukaryotic recombinant protein expression, resulting in a high yield of active protein. The Brevibacillus system is also almost completely free of proteases, allowing for the production of intact protein products.
The Brevibacillus system facilitates disulfide bond formation, which is often required for activity in proteins of eukaryotic origin. In addition, B. choshinensis serves as an excellent host for intracellular protein production, producing soluble intracellular proteins in the cytoplasm without the formation of inclusion bodies. In fact, the Brevibacillus Expression System II often works better than comparable E. coli-based systems for the expression of certain recombinant protein targets.
Using his-tag vectors (pNC-HisE, pNC-HisF, pNC-HisT, pNI-His) allows for quick and easy purification of the expressed target proteins. These tags can be removed by protease treatment following purification.
3. All rights of intellectual property related to this product are owned by Higeta Shoyu Co., Ltd. A copy of the ◊pNY326-BLA DNA product License Agreement can be found by clicking here.
pNY326-BLA DNA is a positive control plasmid for Brevibacillus secretory production. It contains the gene for Bacillus licheniformis α-amylase (~55 kDa) and produces ≥0.1 g secreted protein/L.
Note: This product requires completion of a License Agreement before purchase.
Examples of expressed proteins using the Brevibacillus Expression System II are shown in the table below. High expression levels have been achieved for a variety of proteins (enzymes, antigens, and cytokines) regardless of their genetic origin (bacterial, archaeal, or eukaryotic). Because secreted eukaryotic proteins often depend on intact disulfide bonds for activity, it is generally difficult to produce active proteins using typical prokaryote-based expression systems. However, due to the secretory advantages of the Brevibacillus Expression System II, high expression levels of active recombinant protein are possible even for proteins with extensive disulfide bonds.
Takagi, H., Kadowaki, K. & Udaka, S. Screening and characterization of protein-hyperproducing bacteria without detectable exoprotease activity. Agric. Biol. Chem. 53, 691–699 (1989).
Mizukami, M., Hanagata, H. & Miyauchi, A. Brevibacillus Expression System: Host-Vector System for Efficient Production of Secretory Proteins. Curr. Pharm. Biotechnol. 11, 251–258 (2010).
Sugimoto, S. et al. Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis. J. Appl. Microbiol. 111, 1,406–1,415 (2011).
Takano, T. et al. Expression of the Cycledextrin Glucanotransferase Gene of Bacillus macerans in Bacillus brevis. Biosci. Biotech. Biochem. 56, 808–809 (1992).
Teramura, N. et al. Cloning of a Novel Collagenase Gene from the Gram-Negative Bacterium Grimontia (Vibrio) hollisae 1706B and Its Efficient Expression in Brevibacillus choshinensis. J. Bacteriol. 193, 3,049–3,056 (2011).
Tojo, H. et al. Production of human protein disulfide isomerase by Bacillus brevis. J. Biotechnol. 33, 55–62 (1994).
Yamagata, H., Nakahama, N., Suzuki, Y., Tsukagoshi, N. & Udaka, S. Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor. Proc. Natl. Acad. Sci. USA. 86, 3,589–3,593 (1989).
Yamamoto, A., Fujino, M., Tsuchiya, T. & Iwata, A. Recombinant canine granulocyte colony-stimulating factor accelerates recovery from cyclophosphamide-induced neutropenia in dogs. Vet. Immunol. Immunopathol. 142, (2011).
Yamamoto, A., Iwata, A., Saito, T., Watanabe, F. & Ueda, S. Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF). Vet. Immunol. Immunopathol. 130, 221–225 (2009).
Yashiro, K. et al. High-Level Production of Recombinant Chicken Interferon-γ by Brevibacillus choshinensis. Protein Expr. Purif. 23, 113–120 (2001).


Reconvened AGM Scheduled for Next Monday Night.
After last nights marathon session, the 2013 AGM will reconvene on next Monday evening, 9th December, 2013, beginning at 7.00pm sharp. On the agenda will be the election of Team Managers for our various Hurling and Football teams and to elect members on to our Executive Committee.


text:
2.32 billion monthly users worldwide
Company net worth $138 billion
According to Monster.com, 80% of employers are using social media as a recruitment tool. Jobvite's 2020 Recruiter Nation Survey reports that social media channels most used for recruiting are LinkedIn (72%), Facebook (60%) Twitter (38%) and Instagram (37%).
Many employers will place job advertisements on Facebook. You can find these opportunities by searching for "Jobs on Facebook" in the search window on the site.
After 15 years Facebook is still one of the largest social medias around, however how long is it to stay at the top? With the recent privacy and hacking scandals is the younger generation more likely to go to competitors Instagram or Twitter? What does the future hold for Facebook?
It has over 2.23 billion users worldwide and each second it gains 5 new users. It is largely used as a social/personal tool to connect friends and family across the globe. However it is now also used as a tool for businesses to connect with potential consumers across the network. Especially with small local businesses. There are over 80 million businesses using Facebook, 6 million of these advertise of Facebook as well. This the third visited site in the world beside Google and Youtube respectively.
In the future Facebook needs to gain more of interest with young people in order to maintain relevance. Although Facebook is currently the most popular form of social media the majority of its users are over 30. Although this may be okay in the short term eventually it may quickly become irrelevant if there is no engagement with the younger generation.
Messenger has now been developed into its own app and it the most popular messaging app with over 1.3 billion users. This was first a built into feature within the original app however it quickly expanded and has now developed into its own entity with unique features. Lots of businesses use it as a form of customer service.
Know about all of the social media? Click here to learn how to Stay Savvy.

redpajama_set_name: RedPajamaCommonCrawl


A Cafeteria in a University Campus located near Auditorium, Cultural Center, Mass communication, was expected to become hub of all social activities of the Campus. Due to the extreme climatic conditions of New Delhi, where the summer sees temperatures of above 45 degrees centigrade, and the winters often see temperatures below 5 degree centigrade. Most student canteens in India are not air-conditioned, and are often poorly ventilated, making them very hot and oppressive in the summer and very cold in the winters. This canteen was proposed as a 'Semi open air Cafe'. This allowed to have an ambient temperature for most of the year along with good ventilation, and a variety of degrees of shade from the climate. The design is truly unique and contemporary and we feel, will herald a new age of Architecture for the University.
CafeThe building block has a kitchen block to the east, which is a fully enclosed space to cook and serve in. As One walks along the length of the building westwards, initially the eating enclosure is defined by two walls and a roof, further still the sense of interior is defined by one wall and the roof, further still the space is articulated by only one wall, and yet further still, there is only the floor, and then that too stops continuing.
Throughout this changing sense of interior and exterior, the eating surface and the seating surfaces continue, almost acting like stitches that tie this entire space together.
The idea was to try and blur the boundaries between inside and outside, where these undefined boundaries act as a negotiator between the user and the climate of Delhi.
All the elements of the building are defined distinctly and independent from each other. The walls don't touch the floor and the roof does not touch the walls.
This was the first steel building built at the university campus.
Kitchen: Marble Cladding over kitchen walls comes in handy for this kind of busiest place.
Floor: Kota stone is the hardest locally available stone. Its slabs have been used for flooring, skirting, dado, risers of steps etc. keeping in view the high expected usability of the building.
Perforated aluminum sheet has been used as a ceiling so as to reduce noise levels within the canteen. Lights have been concealed in this ceiling to achieve a clean soffit.
Benches: Precast RCC, with Sheesham wood tops.
Tables: RCC with granite top.


text:
Author: Rica Bouso
Home for the Holidays: How to Make this Season Extra Warm and Fuzzy
The holiday season will be memorable in ways that we never could have imagined. We may be celebrating apart, but we are in this, TOGETHER.
Thanksgiving from a Distance: 6 Ways to Make it Special
While we know that nothing can replace a warm embrace and the special people in our lives eating and laughing at one table, we have put together some resources to bring holiday cheer to this year's thanksgiving.
Donna LaPietra Combines Art and Philanthropy to Build a Better Chicago
Meet the 2020 Library Foundation's Inaugural Civic Award Honoree: Donna LaPietra. For over 20 years, the artist-philanthropist-civic leader has been lending her artistic expertise to bring the stories of several nonprofits to life. Her motivation? To serve the community. Get to know her journey and her story here.
2020 Chicago Public Library Foundation Awards: Celebrating Great Stories in a Year Full of Plot Twists
Stories have the power to create social change and inspire communities. Here are some of the most vital narratives of this generation, written by Isabel Wilkerson, our Carl Sandburg Literary Award honoree, and Nate Marshall, our 21st Century Award honoree.
Bridging the Digital Divide One DigitalLearn Lesson at a Time
"I'm terrified of computers," said Jackie, a Library patron. As everything moves online and digital literacy becomes a lifeline, Chicago Public Library's DigitalLearn empowers Chicagoans like Jackie to turn digital barriers into tools.
Dear Parents and Caregivers: Chicago Public Library Is Here to Help Out
"How do I balance working from home, housework, and homework?" "Am I doing enough to support my child's education?" Navigating the challenges of remote learning can be daunting for students and parents alike. But you are not alone. Chicago Public Library is here to help with free resources that will make learning at home more manageable for the family.
Breaking Barriers to Access: Chicago Public Library Partners with Working in the Schools (WITS) to Empower Early Readers at Home
Access to free, high-quality summer learning programs is more critical today than ever before. Together, Chicago Public Library, Chicago Public Library Foundation and WITS will empower thousands of Chicago families to fight the COVID learning slide by connecting them to an unparalleled network of educational services and supports.
Summer Learning Challenge: Build Stories with Chicago Public Library
Summer is not canceled! It may look a little different this year, the Library's Summer Learning Challenge is here to keep children active, creative, and learning all summer long.
Welcome Back to Chicago Public Library
The Library may look and feel different for a little while. But we are here for you. The Library and the Library Foundation's commitment to empowering Chicagoans and communities navigate the challenges of recovery are stronger than ever. We are all in this together.


text:
Szerencsi Hugó (Békéscsaba, 1926. augusztus 6. – Békéscsaba, 2002. szeptember 24.) magyar színész.

Élete
A Színház- és Filmművészeti Főiskolán diplomázott, majd 1955-ben a Békés Megyei Jókai Színházhoz került. 1957-ben a kaposvári Csiky Gergely Színház, 1958-tól a Pécsi Nemzeti Színház, 1965-től újból a Békés Megyei Jókai Színház tagja volt. 1977-től 1986-os nyugdíjba vonulásáig tagja volt a Magyar Filmgyártó Vállalat színésztársulatának. Sírja Békéscsabán, a Vasúti (Alsóvégi) temetőben található.

Fontosabb színházi szerepei
 Ferdenyakú (Makszim Gorkij: Éjjeli menedékhely)
 Miller (Friedrich Schiller: Ármány és szerelem)
 Mike (Arthur Miller: Pillantás a hídról)
 Thomas Putnam (Arthur Miller: A salemi boszorkányok)
 A burgundi fejedelem (William Shakespeare: Lear király)
 Gratiano, Brabantio testvére (William Shakespeare: Othello)
 Exeter gróf (William Shakespeare: V. Henrik)
 Pantalone, vadászati miniszter (Carlo Gozzi: Szarvaskirály)
 Myska bán (Katona József: Bánk bán)
 Kántor (Bródy Sándpr: A tanítónő)
 Cseresznyés (Molnár Ferenc: A doktor úr)
 Tiribi, udvari bolond (Heltai Jenő: A néma levente)
 Mircse, cigányprímás (Móricz Zsigmond: Nem élhetek muzsikaszó nélkül)
 Bencsik József (Karinthy Ferenc: Ezer év)
 Támadi Miklós, méltóságos úr (Görgey Gábor: Lilla és a kísértetek)
 Ligeti Sándor, táblabíró (Görgey Gábor - Vörösmarty Mihály: Handabasa, avagy a fátyol titkai)
 Kalmár (Tarbay Ede: Mese a tűzpiros virágról)
 Kelemen Félix (Eisemann Mihály: Én és a kisöcsém)
 Kászonyi ezredes (Huszka Jenő: Mária főhadnagy)
 Archibald (Ábrahám Pál: Bál a Savoyban)

Filmjei

Jegyzetek

Jegyzetek

Források
 Kortárs Magyar Színészlexikon, Magazin Kiadó 1991.

 Magyar színházművészeti lexikon

Magyar színészek
Békéscsabaiak
1926-ban született személyek
2002-ben elhunyt személyek

redpajama_set_name: RedPajamaWikipedia


text:
Trade Deadline Day Trades and Rumours
wafflecombine
Re: Trade Deadline Day Trades and Rumours
Post by wafflecombine » Tue Apr 09, 2013 7:54 am
BigTuna wrote: Let's see Mike Smith in a non-trap system.
On this account I have to agree with you BT. Watching the game last night I'm thankful no-one got the bright idea you had to do a shot everytime 5 'Yotes players were simultaneously within 10 feet of the goalie.
Even a pro drinker would have been under the table before the end of the first period.
Location: Carl Junction, MO
Post by tantalum » Tue Apr 09, 2013 8:07 am
Reimer right now is middle of the road (Rask and Schneider are top of the pack right now and should be the leading Vezina votes today). 14 days ago his number weren't very good. he has played well the past couple of weeks and his numbers came up. Is he Cloutier? or is he a top 10 guy? We don't know yet. Last night he got the win but he wasn't good. A couple of goals while not terrible weren't great either. A TON of just terrible rebounds on shots that the Rangers simply knocked wide instead of burying. Yeah the Leaves give up too many shots and the blueline isn't great but some of that shot total comes solely from Reimer being unable to control shots he ought to be controlling. His glove hand is not good. He can go either way but right now it is just as likely he gets lit up in the playoffs....if not more likely. Especially as it looks like they will be meeting the Bruins or Canadians in the first round...two good offensive teams.
The Leaves have a chance to get a reasonable seed for the playoffs. Because of that, IF the goaltending is a major factor to them getting bounced early Nonis should have to answer questions. It's all well and good to appraise the team and not bring in much help if you are going to squeak in. It's quite another if you have a solid position and the addition of an impact player can get you a playoff round win or two. And that, IMO, is why Nonis really messed up on deadline day. He had the chance to add an impact player to a playoff team for nothing, at a position he WAS concerned about (you don't try to sign old goaltenders to big extensions to get them to move and then discuss other trade options if you are happy with your goaltending). He didn't because he gets stuck a bit when a team performs better than expected and he might have to accelerate his plan a bit.
It might work out for Nonis in the end, but I still think it's a mistake to leave an impact player on the table when you could have him for nothing.
We'll see what happens, but nothing I've seen this year would make me comfortable with Reimer in net if I was a Leaves fan.
Post by Doyle Hargraves » Tue Apr 09, 2013 9:42 am
BigTuna wrote:
Blob Mckenzie wrote: Roy has averaged 60 + points a season while Bozak has averaged 40 + points a season. Roy is a much better player.
If anyone thinks Lu would be a backup in TO that person is a fucking idiot.
Roy's 60 point average is from the early part of his career.
Roy averaged 62 points his first 4 seasons. He has averaged 59 points his last 5 seasons. Quite the dropoff there. Like shooting tuna in a barrel.
Not to mention he had one bad year in 2011-12 when he was injured which skews the numbers. But hey you keep on keepin on it's good entertainment.
Location: Old Berlin
Contact Arachnid
Post by Arachnid » Tue Apr 09, 2013 9:47 am
Blob Mckenzie wrote:
This tuna not only has bad taste but tastes bad (spoiled can?!).
I love every move Jim Benning makes
Chef Boi RD
Post by Chef Boi RD » Tue Apr 09, 2013 12:30 pm
Regarding Roy's future, neverminding the Cap hurdles Gillis has with the Nucks this summer, I just don't see Roy being all that excited being a 3rd line centre behind Hank and RK.
DOYLE WOULD'VE RATHER HAD KEVIN HAYES AT 7 YEARS 7 MILLION PER INSTEAD OF J.T. MILLER FOR A LATE 1ST RD PICK
Hockey Widow
Post by Hockey Widow » Tue Apr 09, 2013 1:43 pm
He is an Ottawa boy who has not overly enjoyed playing in the West. Front money right now is he hopes to have a chance to go to Ottawa. But time will tell. Who knows, maybe he falls in love with Vancouver and decides to work out a deal but boy oh boy I can't see the Canucks being able to afford him. He is everything I hope JS becomes and I think in a full season the Canucks will go with JS at third line centre and give him the full year to develop there.
The only HW the Canucks need
Post by ukcanuck » Tue Apr 09, 2013 1:58 pm
Like shooting tuna in a royal biker barrel??
Post by Doyle Hargraves » Tue Apr 09, 2013 2:58 pm
ukcanuck wrote:
Bikers ??? ! Who's wearing the assless chaps ?
I dunno but those things are sooo gay, unless your actually on the range with a horse that is....
Royal dude.... Biker dude....big tuna... Something smells like fish and it ain't the tuna
Post by vic » Tue Apr 09, 2013 4:49 pm
RoyalDude wrote: Regarding Roy's future, neverminding the Cap hurdles Gillis has with the Nucks this summer, I just don't see Roy being all that excited being a 3rd line centre behind Hank and RK.
(next year) MOVE KELSER TO THE WING!!!
Sedin - Sedin - Burrows
Kassian/Higgins - Roy - Kelser
Kassian/Higgins - Schroeder - Hansen
Zamboni Driver
Post by Zamboni Driver » Sun Apr 28, 2013 7:02 pm
Island Nucklehead wrote:
A quality third line centre. We've since acquired a Centre that will allow us a 2A/2B combo. So yeah, there's nothing on Toronto's roster that we need.
Sorry Nucklehead, that's bunk. The Canucks couldn't use a quality 3rd line center and a solid defenceman? Or do you think Ebbett & Barker are what we need?
Island Nucklehead wrote: Derek Roy is better than Tyler Bozak. Even as a 3rd line Centre.
First off, Roy isn't a 3rd line center, a salary of $5 or $5.5 mil + puts him in 2nd line center territory, and 1st line on some teams.
Second, the comparison is rather useless. Maybe we should also point out that Bozak isn't as good as Crosby or Ovechkin?
It isn't a matter of Roy OR Bozak, we could have gotten both, or neither.
The question is what we could get back in return for Lu, and what would be better for the team.
Had we gotten Bozak & Franson for Lu (as was rumored) there's no reason why we couldn't ALSO get Roy as a rental, to make a really solid push in the playoffs.
Derek Roy is better than Tyler Bozak. Even as a 3rd line Centre
Far better and Luongo is far better than Reimer.Not even sure why any of this is debatable. Reimer has better numbers in a lockout shortened season, BFD. He has never sustained good numbers for any length of time.
Blob, the reason is this:
1.) Yes, I agree that Reimer is not the same established quality reputation as Lu, (and maybe will never be) The point is that the beginning of the season is the time to do a deal, when the Laffs are not sure that Reimer can get them in the playoffs, and TO is somewhat desperate to break the 9 year drought. By the trade deadline (or after) they are not in a desperate need, as they are confident that they have a goalie who can get them into the first round, at least.
2.) I don't disagree that Roy has better numbers than Bozak, but Bozak is the better player FOR THE CANUCKS.
Why? - Because unlike Roy, we can actually afford to resign Bozak, and as he's from Western Canada and having played hockey in BC, he's more likely to want to stay here.
Don't get me wrong, Roy is a quality center, it's just that we can't afford him unless he is willing to take a big haircut, which I just don't see happening.
Canuck-One wrote: Luo didn't get sentenced to hockey banishment in Toronto and we'll get better players back in the summer than the ridiculous crap that they say MG was willing to accept. .
Canuck One, supposedly at the beginning of the season Gillis wanted a first, a top 6 (top 4) roster player, and a blue chip prospect. The Leaves were supposedly offering Bozak & Franson. By the trade deadline the offer had gone down to Scrivens & a 2nd & 3rd, provided Gillis would eat some of the salary. I can't see anyone making a much better offer, now that Toronto has blabbed what the going price was, we'll get some more lo-ball offers.
And the other downside is this, IF we had gotten some players at the beginning of the season, it would improve our depth in the playoffs, when third line scoring is often what's needed if the top lines get shut down.
Do you think that Ebbett & Barker/Ballard/Alberts are going to step it up in the playoffs?
The other factor is this, had we moved Lu's contract at the beginning of the season, we could have made some offer sheets or signed UFAs this summer when some quality players will be looking to move. Unless Lu's contract can be moved quickly then our hands are tied.
Bottom line - If Gillis this summer gets the kind of offer that supposedly he's looking for (a 1st, a roster player & a blue chip) or even close to that, I'll be shocked.
Island Nucklehead
Post by Island Nucklehead » Sun Apr 28, 2013 7:46 pm
Zamboni Driver wrote:
Why is that "bunk"? You cherry pick a quote from 3 weeks ago, when we acquired a CENTRE at the deadline, and now because of a coaching decision made post-deadline you figure we coulda used another Centre? Nice. If AV was using players at their correct position, as most of us assumed he would with that trade, Ebbet wouldn't be playing. Sedin-Kesler-Roy-Lapierre-Ebbet-Schroeder.
Same thing for Barker. Edler, Garrison, Hamhuis, Bieksa, Tanev, Ballard, Alberts, BARKER. So yeah, when guys get hurt (Bieksa, Tanev) or healthy scratched (Edler, Garrison, Hamhuis) we'll see Barker. He wouldn't play on a healthy back end.
Point being (again): why would we trade Luongo for players we don't need?
Island Nucklehead wrote: First off, Roy isn't a 3rd line center, a salary of $5 or $5.5 mil + puts him in 2nd line center territory, and 1st line on some teams.
No shit. But on this team he's third. Sedin and Kesler are both better at the Centre position.
Why is the Roy/Bozak comparison useless? These were both players that could have been targetted by the Canucks as deadline pickups. Roy was relatively cheap to acquire, and we got to keep Luongo. If you still have a massive boner for Bozak (who's also a rental), we can sign him in the offseason.
I'm also curious who would back up Schneider if we dealt Lou in your fantasy world. Hell, for all we know Luongo may start in the playoffs. Right now, it seems like hanging on to him AND filling our need at centre was a prudent move.
The bottom line is (assuming Toronto would consider trading Bozak) we had our choice of rentals. Roy >> Bozak. We picked up the better player for us now. We can still pick up the cheaper player in the offseason, for nothing but cap space. Seems pretty win win to me.
Who? Luongo will more than likely go at the draft, well before UFA or offer sheet season. Even if when we do move him, chances are we're not going to have the cap space to compete for any of the bigger names.
Why is that "bunk"? You cherry pick a quote from 3 weeks ago, when we acquired a CENTRE at the deadline, and now because of a coaching decision made post-deadline you figure we coulda used another Centre? Nice.
Roy does look good with Kesler & Higgins though, I'd just like to see an upgrade of Ebbett.
Island Nucklehead wrote: If AV was using players at their correct position, as most of us assumed he would with that trade, Ebbet wouldn't be playing. Sedin-Kesler-Roy-Lapierre-Ebbet-Schroeder.
We've needed a better center than Ebbett all year, and having an additional scoring threat during the playoffs is certainly something that we can use.
If we get into the Conference finals and don't lose anybody to injuries, then you'll be right.
If we lose in the first two rounds due to lack of depth, or because of too many injuries, then having additional quality players would be sorely missed.
An additional R-shooting D man with some scoring skill can't help either.
Yep, but I don't see how we can keep two 2nd line centers, that's the dilemma going into next season.
Why is the Roy/Bozak comparison useless? These were both players that could have been targetted by the Canucks as deadline pickups.[/quote]
My point was that we would have been better off trading for him at the beginning of the season, not at the deadline.
Getting Roy as a rental was a decent trade, having Roy AND Bozak would have been better than Roy & Ebbett, that was my point.
Who? Luongo will more than likely go at the draft, well before UFA or offer sheet season. Even if when we do move him, chances are we're not going to have the cap space to compete for any of the bigger names.[/quote]
No, I actually don't think we have a great need for top 6 or top 4 players, but we need some better supporting cast.
Post by Island Nucklehead » Mon Apr 29, 2013 6:46 am
Zamboni Driver wrote: Roy does look good with Kesler & Higgins though, I'd just like to see an upgrade of Ebbett.
All these points completely forget that there is a salary cap in the NHL.
The Canucks were on the verge of cap trouble all season. We knew Kesler would be out for a while, so while we could have added someone using his LTIR, we'd have to get under the cap when he returned. Booth's injury is what allowed us to go get Roy. Even towards the end of the season we saw Ebbett over Schroeder most likely because he was cheaper.
Depth is the key thing here. We can't just add whoever we want whenever we want. Are we in trouble if some of these guys wind up playing critical minutes for us? Absolutely. But it's the same for any team.
Adding Roy, Bozak and Franson would be great if we didn't have the salary cap or the roster limit (or backup goalie) to worry about.
Post by Hockey Widow » Mon Apr 29, 2013 9:13 am
All of us have known for some time that sooner or later more of our younger players need to step up for us to be cap competitive. Tanev is the only real player from our younger players. Kassian is on the edge and has been for the most part a daily player. We needed more from JS this year and according to AV we didn't get it. Corrodo seems like he is on the verge of being able to do that and next year may be his season to break into the line up. If he can do that then next year we will have both he and Tanev on very good cap hits.
Up front we will likely lose Booth and Raymond. We need a couple of younger players to be good enough to fit into the line up.And we need one of our goalies to be ready for back up duty.
MG has said repeatedly, and I believe him but call me stupid, that he could have traded Luongo a number of times if he was willing to take cap and term back. He could not afford to do that moving forward.
So while I agree it would have been ideal to trade Luongo at the start of the season I just don't know if a deal was there. We have a lot of rumours but nothing of substance. People say we could have had Bozak and Franssen but I have also heard that the Leaves wanted us to take cap and term along with anything else we got. But since I don't know what MG was offered I choose to believe him when he says there wasn't a deal there.


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The Money Quest: Is it worth Investing in Kisan Vikas Patra (KVP)?
Is it worth Investing in Kisan Vikas Patra (KVP)?
Other than the tax-saving debt instruments available for investments (e.g. PPF, NSC, Bank FDs), which debt instruments can be regarded as good from medium to long term investment point of view?
Due to the current low-interest rate regime, small savings instruments have once again started looking attractive. KVP is also a small saving instrument available at post offices offering a pre-tax return of 8.41% per annum (your money doubles in 8 years & 7 months).
1. Easy to purchase: Available in denominations of Rs 100, Rs 500, Rs 1000, Rs 5000, Rs 10,000 and Rs 50,000. No a/c opening hassles are involved. Just go to a post office fill a form, hand over the cash or cheque / DD and you're done. Further there is no limit as to number of KVP certificates you can purchase or maximum amount you can invest.
2. Post-tax Yield: The interest is taxable on annual basis (although no TDS is involved). The post-tax yield from KVP depends on the marginal tax rate that will be applicable to you.
4. Loan facility is also available against KVP by pledging it with the bank.
No doubt PPF, NSC, Tax-saving bank FDs have an edge over KVPs due to associated tax benefits. But once you exhaust your section 80C limit & PPF investment limit, investing in KVP becomes an attractive proposition.
PPF maintains its superiority over all other small-saving schemes (even ignoring the section 80C tax benefit) because the post-tax yield of PPF is substantially higher than all other debt instruments. PPF is the only debt instrument (other than the EPF), where your interest income is completely exempt. Accordingly the tax-free interest of 8% from PPF is much better than 8.41% taxable interest from KVP. However, if your total income is either nil or less than basic exemption limit, then KVP will score over PPF.
Even after implementation of Direct Tax Code (DTC) which will make the PPF withdrawals taxable, post-tax returns of PPF will be better than KVP.
1. Returns: Yield is 8.40% from KVP as against 8.16% from NSC.
2. Taxation: First, NSC is one of the eligible instrument u/s 80C i.e., the amount of your income invested in NSC gets exempted from tax. Second, returns of both the instruments are chargeable to tax. But unlike KVP, NSC interest is again eligible for deduction u/s 80C. But if your section 80C limit is already exhausted, then this tax benefit offered on NSC is of no good.
In short, considering tax benefits NSC is undoubtedly better than KVP (Note: there won't be any more tax benefit on NSC after implementation of DTC). However, if tax is not the criteria, then KVP returns are a little better than NSC and with a longer investment period of about 2.5 years.
Over the last year, returns offered on bank FDs are steadily coming down. At present 5- to 10-yr bank FDs are offering interest in the range of 7.25% to 7.75%.
Although the YTM of 8.58% (accompanied with interest rate risk) on the L&T finance NCD 2nd issue in February 2010 was almost at par with KVP, the duration was 3 years as against almost 9 years of KVP.
Coming to medium and long term debt mutual funds, the 5-Yr category average returns are somewhere between (As on March 2010) 6 and 7 percent with some tax benefit due to tax arbitrage. Moreover, when there are chances of increasing interest rates, it is very risky to invest in long term debt funds.
So, due to above reasons, KVPs have started looking very attractive debt instrument offering relatively better returns without any risk.
In a nutshell, if you've completed your tax savings and are looking for a debt instrument offering assured good returns combined with safety and liquidity then KVP is a good choice.
In other words, invest in KVP if you're done with your tax saving investments and further exhausted your PPF investment limit (including your spouse & children) but make up your mind that you'll remain invested till the maturity because if you make a premature exit, your effective yield will be considerably lower (the facility of making an early exit can be exercised in an emergency by sacrificing some returns).
2. Why should you invest in gold ETFs?
In KVP, you would then have to make a bulk amount as investment, right, unlike a PPF for proper benefit? If you deposit 500 Rs today and then it becomes 1000 after 8 yrs 7 months, then it is of no use. But in a PPF, different amounts can be deposited as and when required. Will that not be an easier and more flexible thing to do?
Very correct…although it is also possible to invest different amounts in KVP over a period of time, but then unlike PPF each KVP certificate will have different maturity date.
Hi, If i invest Rs one lakh in KVP, it becomes 2 lakhs in 8.7 years.
Could you tell me exactly out of that 2 lakh what is the amount that would be deducted as tax.
There's no tds in case of KVPs.
1. How much amount i can get now.
2. I can not go Dewas (MP) from where i purchased this KVP just because of this reason so pls suggest some easier way.
I am a housewife and want to invest 100000 rs,but i am worried that TDS would be deducted on the returns i get n will eat away the fruits of interest as i am not filing my returns. could u pl suggeststhe best way for my investment where TDS would not be deducted????
i m bhanuben m. fachara.i had purchase 5 1/2 year kvp in july 2003 for 8 year plan. so my que. is how can i my money back after complete my plan period ? Can i see my kvp plan update on online ? plz suggest me earlier as possible.
With KVP scheme taken off, what are the alternatives now?


If you see this error in kibana on an agent. It could be for a number of reasons.
Follow this process to figure it out.
Agent buffer on the client is full, which is caused by flood of alerts. The agents have a buffer size to keep resources on the clients consistent and minimal. If this fills up then kibana will stop collecting data.
Logs can stop generating if elastic-search partition reaches 85% full and put the manager into read only mode.
Ones you have ensure there is adequate space you will need to execute a command in kibana to get it working again.
In kibana, go to dev tools and put the above code and play the code.
Alternative command that does the same thing.
Oct 18 12:25:53 waz01env: Completed.
Oct 18 12:25:53 waz01systemd: Started Wazuh manager.
If any of these are failed restart them.
# systemctl stop logstash…# systemctl stop filebeat (this should not be installed on a stand alone setup as it causes performance issues.
5. Restart Logstash & run the curl command to ensure its not readonly.
1. Check last 10 alerts generated in your Wazuh manager. Also, check the field timestamp, we must take care about the timestamp.
…2. If the Wazuh manager is generating alerts from your view (step 1), then let's check if Logstash is reading our alerts. You should see two processes: java for Logstash and ossec-ana from Wazuh.
alerts in that section of Kibana itself. If there are alerts from today in the Discover section.
Reason 4: Agent buffer is full due to flood events. If this occurs events are not logged and data is lost. We want to drill down on a specific agent to figure out what is causing the issue.
This should provide a log an output to show that the agent is logged in the indices for that day. If this is successful then we know that the logs are coming and kibana is able to communicate.
It should show you a positive number, and that number is the number of lines in the audit.log file. Note down it.
2018/10/17 13:36:03 ossec–syscheckd: INFO: Ending syscheck scan (forwarding database).
If you still see the agent buffer full after these steps then we need to do debugging.
2018/10/17 00:09:08 ossec-agentd: WARNING: Agent buffer at 90 %.
2018/10/17 00:09:08 ossec-agentd: WARNING: Agent buffer is full: Events may be lost.
2018/10/17 12:10:20 ossec-agentd: WARNING: Agent buffer at 90 %.
2018/10/17 12:10:20 ossec-agentd: WARNING: Agent buffer is full: Events may be lost.
2018/10/17 14:25:20 ossec-logcollector: ERROR: (1103): Could not open file '/var/log/messages' due to [(2)-(No such file or directory)].
2018/10/17 14:25:20 ossec-logcollector: ERROR: (1103): Could not open file '/var/log/secure' due to [(2)-(No such file or directory)].
2018/10/17 14:26:08 ossec-agentd: WARNING: Agent buffer at 90 %.
2018/10/17 14:26:08 ossec-agentd: WARNING: Agent buffer is full: Events may be lost.
2018/10/17 14:28:18 ossec-logcollector: ERROR: (1103): Could not open file '/var/log/messages' due to [(2)-(No such file or directory)].
2018/10/17 14:28:18 ossec-logcollector: ERROR: (1103): Could not open file '/var/log/secure' due to [(2)-(No such file or directory)].
2018/10/17 14:29:06 ossec-agentd: WARNING: Agent buffer at 90 %.
2018/10/17 14:29:06 ossec-agentd: WARNING: Agent buffer is full: Events may be lost.
Ok, let's debug your agent events using logall_json in the Wazuh manager instance.
Login using SSH into the Wazuh manager instance and edit the ossec.conf file.
5. Now we can take a look into events in order to clarify what is flooding the agent "013".
Once you have the log is seen, you can disable logall_json and restart the Wazuh manager.
From the above log we can see that kubernetes is sending a lot of events to the agent causing the buffer to fill up. To solve this we particular issue from happening in future. We can disable this at the client level or the global level.
– Login using SSH into the Wazuh agent "013" instance.
– Edit the file /var/ossec/etc/ossec.conf, and look for the rootcheck block, then put a <ignore> block for that directory.
Option 2. Check in which group is your agent and edit its centralized configuration.
– Login using SSH into the Wazuh manager instance.
The solution #1 takes effect immediately.
it auto restarts itself automatically and then it applies the new configuration. It could take a bit more time than solution #1.
The above link talks about how to prevent from being flooded.
Now the agent should show correctly in the 15min time range. If a bunch of client had the issue then you need to use ansible to send out a agent restart on all clients or setup a cron on all the machines to restart the agent every 24 hours.
Ansible adhoc command or playbook.
The first step to setting up Wazuh is to add the Wazuh repository to your server. If you want to download the wazuh-manager package directly, or check the compatible versions, click here.
Python >= 2.7 is required in order to run the Wazuh API. It is installed by default or included in the official repositories in most Linux distributions.
Filebeat is the tool on the Wazuh server that securely forwards alerts and archived events to the Logstash service on the Elastic Stack server(s).
The RPM package is suitable for installation on Red Hat, CentOS and other modern RPM-based systems.
This guide describes the installation of an Elastic Stack server comprised of Logstash, Elasticsearch, and Kibana. We will illustrate package-based installations of these components. You can also install them from binary tarballs, however, this is not preferred or supported under Wazuh documentation.
In addition to Elastic Stack components, you will also find the instructions to install and configure the Wazuh app (deployed as a Kibana plugin).
Edit the file /etc/logstash/startup.options changing line 30 from LS_GROUP=logstashto LS_GROUP=ossec.
If you want to download a different Wazuh app plugin for another version of Wazuh or Elastic Stack, check the table available at GitHub and use the appropriate installation command.
By default, the communications between the Wazuh Kibana App and the Wazuh API are not encrypted. You should take the following actions to secure the Wazuh API.
In order to enable HTTPS you need to generate or provide a certificate. You can learn how to generate your own certificate or generate it automatically using the script /var/ossec/api/scripts/configure_api.sh.
In case you do not need to acces to the API externally, you should bind the API to localhost using the option config.host placed in the configuration file /var/ossec/api/configuration/config.js.


Joseph E. Whalen BRANDON — Joseph Edward Whalen, 82, died Thursday, March 7, 2019, at Rutland Regional Medical Center. Funeral arrangements are pending at the Miller & Ketcham Funeral Home in Brandon. A complete obituary will appear in a later edition of the Rutland Herald.


Within the discussion of ethics and economics some have considered designing a code of ethics for economists. But the idea of such a code is potentially problematic from a pluralist standpoint. Some possibilities are discussed here to show that any code concerning the behaviour of economists presumes a particular view of human nature and thus of professionalism. Further, issues of socio-economic power in the profession pose problems for the interpretation and implementation of some possible principles, notably those referring to standards of competence and truth-seeking. It is therefore concluded that any code of ethics should take the form of general guidelines, with primacy given to the ethics of pluralism: tolerance, even-handedness and open-mindedness, on which the interpretation of all other ethical considerations rests.


A bubble curtain being used in Holandsfjord to prevent the formation of ice. Photo: SINTEF.
"It's also possible to deploy large-scale systems, for example by installing pipes across the entire Yucatan Strait, or extending them from the mainland along the coast. There is no shortage of possibilities", says Eidnes. (View the map).


text:
Misdemeanor love
Sorry, no definitions found. You may find more data at misdemeanor.
I wonder if Viriginia provides defendants with counsel in Misdemeanor 3 prosecutions.
The Volokh Conspiracy » At Least About 200 Criminal Libel/Slander Prosecutions in Virginia from 2001 to 2008
As you may have heard, Microsoft recently launched a clothing line of hipster t-shirts including one called The Misdemeanor featuring Bill Gates 'infamous mugshot.
Megite Technology News: What's Happening Right Now
Mayor Coleman Announces Policy Not to Pursue Certain Misdemeanor Charges Against Journalists
20 « September « 2008 « Niqnaq
quietly dropping all charges
The nominees for best video at this week's MTV Music Video Awards were 50 Cent, Eminem, Missy "Misdemeanor" Elliott, and Justin Timberlake.
CNN Transcript Aug 30, 2003
COOPER: And Missy "Misdemeanor" Elliott is in this.
CNN Transcript Dec 5, 2003
Timbaland: This artist/producer first emerged in the public's consciousness through his innovative work with Missy "Misdemeanor" Elliott (another influential '90s artist) and the late Aaliyah. 1997's "The Rain (Supa Dupa Fly)" still sounds hypnotic, with its offbeat tempo, Elliott's distinctive rapping style, and her singing a sample from Ann Peeble's "I Can't Stand the Rain."
All articles at Blogcritics
Missy 'Misdemeanor' Elliott assaulted Tel Aviv Thursday night with a concert that could be called soulless at best.
Haaretz.com headlines RSS
They call her "Misdemeanor," but Vampire Weekend (2008)


Rut Jesus is a PhD student at the Center for Philosophy of Nature and Science Studies, University of Copenhagen, Denmark. She is Portuguese-Indian, 27 years old, and studied Physics and Philosophy at Harvard University. She is particularly interested in 'productive' communities, from coop living to Wikipedia, in bridging different disciplines, including the sciences and the (performing) arts, and in promoting dialogue.
This exploratory study investigates the bipartite network of meta articles linked by common editors in Wikipedia: and Help: namespaces (and their talk pages) of the South American Wikipedias (ES, PT) and MetaWiki. We construct a bipartite network, and from it, overlapping cliques of densely connected articles and significant editors (who edited at least 10 edits per each article) to study the communities that organize, discuss, and lead those Wikipedias. We compare the 'Café' and 'Esplanada' to the 'Village Pump' and to MetaWiki and see at a meso-level the appearance of coordination areas, policy making and general guidelines.
This page was last edited on 8 September 2009, at 19:39.


DreamHost offers an FTP client in the panel called 'WebFTP' that you can use to connect to your webserver. The instructions below describe how to use this feature in the DreamHost panel.
Your site must be Fully Hosted in order to log in to it.
Under your domain, click the 'WebFTP' link. This automatically logs you into the WebFTP server.
The page automatically loads the directory.
This returns you to the directory just above the current directory.
Uploads are limited to 1GB.
Log into WebFTP using the instructions in the above section.
To upload files, click the upload icon on the bottom left.
A pop-up window appears allowing you to locate the file/folder to upload.
Downloads are limited to 1GB.
This section explains how to download files or folders from the web server to your computer.
In your web directory, right-click the file you wish to download.
You can also click the file to highlight it, then click the download icon on the bottom toolbar.
Depending on your browser, you are either prompted to confirm the download or it downloads automatically (no prompt).
Folders are downloaded as .zip files.
This option allows you to download a file from an external location by using the file's URL.
In the bottom toolbar, click the 'Fetch' icon.
Click the OK button to download the file.
Right-click a file to open an editing options menu.
In the menu, select 'Edit'. The source editor opens where you can make adjustments to your file.
After you finish editing the file, click the Save button on the bottom right to save your changes.
When finished, click the Close button to go back to the previous listing of files.
You can create a file or folder within WebFTP.
In the bottom toolbar, click the Create icon.
From the menu, select either 'New File' or 'New Folder'.
A pop-up displays where you can name the new file or new folder.
Enter a name for the file or folder, and then click theOK button to save it.
If you are creating a file, the file editor opens where you can add content to the file.
You can change the permissions on a file or folder within WebFTP.
From the menu, select 'CHMOD'.
In the pop-up, adjust the permissions as needed. You can also type in the number value for the permissions.
From the pop-up window, select 'Delete'.
Article last updated April 11, 2019 17:21 PST.


Every month, I hear from people that have fallen victim to "Common scams".
fear in you! Unfortunately, I am sure I will add to this list as time goes on.
he will get you through it!"
- Request for cash or to send money Western Union.
$10) IF this is your thing it is recommended that this be done by you.
- Paying someone for Meditation.
- Paying someone for prayer work.


text:
'Do not use social media platforms to spread dirt, fake news'
By DB POST Digital // August 30, 2018
Varanasi: Prime Minister Narendra Modi Wednesday requested people not to spread dirt on social media saying it does not behove a decent society. In a video interaction with Bharatiya Janata Party workers and volunteers from Varanasi, his Lok Sabha constituency, the prime minister said everybody should train themselves to never use social media platforms to "spread dirt" but the many good things that are around them.
He added that spreading of false messages on social media is dangerous for society. "People at times overstep the bounds of decorum. They hear or see something false and forward it. They don't notice that how much damage they are doing to society. Some people use such words that do not behove any decent society. They say or write anything about women," said Modi in response to a question.
He asserted that the issue is not about any political party or ideology. It is about 125 crore Indians and everybody should train themselves for never spreading dirt through the social media but share good things around them, the PM said.
Modi also stressed on the need to create an atmosphere of positive news about the country and share information that strengthens the society as he lamented that a quarrel between two families in a 'mohalla' becomes a national news nowadays. He called for sharing videos highlighting the "changing face of India", saying the country is now witnessing "historic and unprecedented" progress.
Was Aadhaar must for girls to tie rakhi to PM? Fake news goes viral
Was Aadhaar card mandatory for girls tying rakhi to PM Modi? A post is going viral on social media, claiming that authorities allowed only girls and women who had their Aadhaar card to tie rakhi on PM Modi's wrist.
On Raksha Bandhan, women and children on Sunday across various walks of life tied rakhis to the wrist of Narendra Modi.
As part of security protocol, girls and women present at the PMO were asked to show some ID. However, furnishing Aadhaar card was not mandatory for girls and women.
The fake claim was based on pictures shared by official website of PMO India and the Press Information Bureau (PIB).
Rakshabandhan 2018
About DB POST Digital
Staff Writer at DBPost.com


Please elaborate in what terms you need help. This looks to have no loads. Also what kind of support are these, pinned or rollers?
They have roller supports. I want to determine the stability, strength and stability of the structure.
As far as I can understand, it cannot be mobile both directions at a time.
Also at lease one support needs to be pinned for the structure to be stable.


I've made a number of orders but only had one customer feedback/review email sent to me. Having said t hat the products I've received have been very good quality and I've had no major issues. A couple of years ago I received an item t hat had a broken piece and they replaced it without me having to ask. So far, really good customer service. They are my go to online shop for general period costuming.