Hello dear Twin Flames! Welcome brave souls – you who are going through the most dramatic and healing relationship you will ever know.
We want to tell you that our Twin Flame experience pushed us through the unthinkable, and it was absolutely worth it. Infinitely so.
Once upon a time, we were blindsided by the deepest LOVE, and now all of our blindspots have been removed. We have purged everything that used to keep us disempowered. We are living without guilt, shame, judgment, or fear. And we are FREE, as we are all meant to be – free to let the soul shine through human form. FREE to love each other in a blissful otherworld.
And we would do it all over again – what we went through to be together. In fact, we were never going to stop. We were going to spend the rest of our lives trying, no matter what it took. Because when the soul calls, the frail human ego and its house of cards – no matter how important they seem, do not stand a chance. You know it. They never will. The house will fall eventually. Why prolong your destiny?
And can you really? Can you put off what calls every fiber of your being? Can you resist the tsunami pull of your heart and soul, and the only one who lights up your world? Because the longing that your Twin Flame unleashes is the same longing you have felt since you were a teenager – to know love as you've always dreamed. To know yourself.
All of this is a grand homecoming for every one of us – even those who fall in LOVE's wake. Even those we must leave so that they remember their own LOVE. And yes, even those whose Twins have run.
We chose our homecoming sooner rather than later. And even though disaster ensued longer than we could barely take, lo! heaven is here on the other side. The same heaven that waits for you, courageous one. The same reunion with yourself, which means reunion with the Divine. Yes, it's here, just on the other side of healing, with your one-and-only perfect Other Half.
Twin Flame love feels more blissful than you can hold. It wakes you up to pure joy and gives you back yourself. But this bliss also highlights what's been keeping you from it. It intensifies your limitations so that you finally heal them. So that you are ready to permanently live in immense and ever-joyful love.
Your Twin Flame relationship is acclimating you to bliss. It is challenging you to stop blocking yourself from it. And at the core of this clearing is a regaining of self-worth. You are removing what's in the way of seeing how divinely perfect you are. And how worthy you are of total, ecstatic joy.
That is why your Twin Flame relationship is a process. It is a series of monumental challenges that ask for courageous steps. And through these steps, you are pushed to finally love yourself. Because self-love is vital for your Twin Flame union. For until you love yourself, you won't be able to stand the ecstasy of permanently joining with your Twin. In fact, such bliss will feel downright unbearable.
Until you love yourself, you won't be able to sit next to your Twin for long. You will start to squirm and ask, "Who me? I can't possibly deserve this joy!" You will come up with several reasons why you don't, and your limitations will be laid bare. And this will feel so intense and unjustly cruel – though the purpose is to finally heal.
Such discomfort can feel impossible. It might tempt you to blame your Twin or deny your love. It might spur you to make excuses, cling to your old life, and run for the nearest door – run from what cries out to be loved in yourself.
Indeed, the emotions that your Twin triggers can feel nightmarish. And you might think that it's all their fault. Your Twin's behavior might even back this up! But they are giving you the opportunity for ultimate healing. Your Twin is helping you to shed your own feelings of unworthiness until you find yourself again. For only then can you bask in the balmy acceptance of unconditional love. Only then can you stand your gorgeous relationship and bathe in its sacred bliss.
Imagine a partnership with zero insecurity. Imagine a life where you feel completely worthy of the greatest love at every moment of the day – something you may not have realized was challenging! Imagine what it's like to rejoin with your Twin and feel total ecstasy. This is what you are being prepared for. And it is exponential joy. We know because it has happened to us.
For all of you struggling to be with your other half, what you seek is more alive than anything you've ever known, and you are being guided to have it again. Because expansive bliss is your true essence and what you are made of. Your human self simply forgot this for a time. Your Twin is here to remind you.
You are being readied for what all humans want – awe-inspiring, gorgeous, romantic love. The kind you've always known was real in your heart. The kind you will fully know when you fully love yourself. For then you will resonate with your sacred union and realize ultimate bliss. You will flourish in your immaculate partnership with your utterly perfect other half.
If you are lucky enough to have found your other half on earth, then deep down you know with complete certainty that this is the only partner for you. You know that the magnitude of your love must be honored, no matter what that means in human terms. And you also know that if you don't honor this love, you will regret it immensely. You will endure the most painful reckoning on your deathbed.
Because your soul cries out to be with your other half. Nothing can take this longing away. Not even the tremendous obstacles you face in order to be together. And it turns out that these obstacles are designed to help you unite.
Your Twin Flame challenges are designed to help you love yourself unconditionally. This makes you whole and able to permanently unite with your Twin. This is what every difficulty between you is challenging you to accomplish. And this is what your incredible longing is guiding you towards. You are being asked to feel the same life-changing, unconditional love that you feel for your Twin, for yourself.
Your Twin Flame challenges are breaking down the ways you have not loved yourself. They are obliterating your old methods of coping, your human conditioning, and your ego. Once you are truly free from these forms of control, you will remember the immaculate perfection you've always been. You will finally, fully embrace self-love.
And you will become utterly empowered. This is what true, divine power is – not chasing goals to make your ego feel better, not looking outside of yourself – even to your Twin! But awesome, full-body LOVE that guides you fearlessly towards JOY. This brings a whole new kind of certainty and confidence to your life. You become so strong with love, from the inside out, that you flourish, unbridled. You fulfill your life's purpose out loud!
The infinite love that you feel for your Twin is calling you to do this. It is calling you to love yourself so that you can shine. This means that you will have to face the ways you aren't loving yourself head on. These things cannot hide any more. Not even in your beautiful relationship.
Such growth cannot be avoided. It will keep calling you with longing and separation – with the ecstasy of joining and the agony of arguments, until you face what begs to heal within. Your Twin Flame relationship will push you to stop living for others and start living for yourself. It will constantly urge you to honor yourself with unconditional love.
You don't want to end up on your deathbed having missed this most golden of all opportunities. You don't want to go back to your old ways, no matter how comforting they have been. Because you came here to do this, and in so doing, help humanity.
It is time to go for what you really, deep-down want – the love that all people dream of: the infinite bliss that you share with your other half. And the full realization that it comes from within you. It shines from the inside out.


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redpajama_set_name: RedPajamaGithub


Small Shop - will it work . . .
Poured a slab (7) years ago for a general purpose "shop". Not necessarily woodworking. Never built the shop, threat of moving kept me from doing so. Now (7) years later I want to get into woodworking & there sits that slab. It's 22' long by 20' wide. . . Can I operate a woodworking shop without "everything on wheels in that amount of space. Would be like to build bookcases, smoking stands, tables of all sizes & some cabinets. Do I need to pour more slab?
Will it fit and work or do I "need" more slab . . . . suggestions please . . . I figure I get one shot at this . . .
If you can afford it, I'd make it bigger--particularly, longer. But to decide, I'd suggest taking a plan and sketching out the equipment you have and expect to get and lay them out w/ movable outlines, trying to see how the operational characteristic would be...particularly pay attention to outfeed areas and sufficient clearance for sheet goods, etc.
Graph paper works great for doing your layout. Gives you a good idea for what space you have to work with and how much space your tools will take up. You can usually get all the dimensions from a catalog or website too.
A few things I did.........I put my grinder on a fold down table........Mounted it to a piece of plywood and mounted that to the wall with two heavy duty hinges.....When I don't need it it folds down and my compressor sits in front of it. I'm planning on putting a dove tail jig on a similar set up, but instead of hinging up and down it will pivot like a door on the wall.....When it swings into place two drop in bars will lock it tight....finish up and fold it back out of the way.
I have approximately your size for my working area in the basement. Actually, mine is a bit narrower than 20' I didn't want my tablesaw on wheels, so I put that in the middle of the length, put against a wall. Put the fence side of the tablesaw against the wall. Leave enough room for 8' in front of the saw and 8' out of the saw. Ideally, of course, more is better, but that's the minimum. Then on the outfeed side of the saw, I built and outfeed table slightly bigger than 8' by 4'. I store all my sheet goods below it. It's also a work table for gluing stuff up, etc. The planer and other desktop tools get pulled off the shelf and used on that table. I have a lathe and drill press against another wall, away from the saw. I did put my bandsaw, jointer, and drum sander on mobile bases.. but it's really not that much of a pain to move them around.
It can be done in your space. You might have to compromise and put a few things on mobile bases though. I'd never put the table saw on a mobile base unless I was extremely tight on space though. I'm sure it's safe, but it's a PITA, because that's the one tool you use a lot. You probably have enough additional space (compared to me) to not have to put your bandsaw on wheels.
one more suggestion. If you can find a place to store your wood in an area other than the shop, it's helpful. Even if you only have about 50-100 board feet, that's a pile that will get in the way if it's on the floor or even on a shelf. Try to steal a little garage or basement space for that.
My space is 20x24 (interior dimensions) less a stairwell... it about the same or a shade bigger. I have big item on your list except the compressor... and I have the 50" rip capacity which takes up some extra space.
Do I wish I had more... heck yes, but it is not claustrophobic. I also do not have room for much wood inventory in the shop.
As other have said, get out the graph paper and pay attention to infeed and outfeed lanes. Share them between tool and even stack them vertically. Make sure that your lanes don't smack into a bench. If you like your benches high, like 36"; you had better raise up you stationary tools to at least that level.
Cheat and use dorways or windoes as in/outfeed space. Once in a blue moon you will want to rip a 10' board in your saw. If opening a door enables that, it's a bonus.
As for height, I would say that 9' is the magic number. You want to be able to manipulate sheet stock without whacking a light fixture. Of course, more is better.
A skylight or two is really nice.
I work out of an area about that size with more than both your lists combined and now do this for a living. That said putting wheels on everything will be cheaper than making you area larger and will make the area that you have seem twice as large. FYI I used to have a dedicated 12" Delta CMS and it truly wasted more space than it was worth. The saw now sets in the store room and I only use it on location and never in the shop.
The first question to ask yourself is "what is the largest piece of timber " for each saw.
If you are regularly cutting 8 x 4 sheet stock your slab is a little undersized, or you need very careful layout.
If you typically use smaller pieces it is plenty big enough.
I like mobile bases on equipment for two reasons. First is cleaning. Second is to get extra clearance for odd large jobs.
Paper and pencil to layout floor plan. If the slab is in place some big cardboard boxes to simulate equipment will be a big help.
Plan carefully for access doors. I rip 10' long stock by opening the garage door.
Your slab is probably big enough with a little planning and some mobile bases.
Consider moving the compressor and dust collection outside the shopspace in a well ventilated addition. These items require a fair size footprint and make a lot of noise.
I'd KILL for that much space. My shop is a 12x14 shed with a 7' roof.
You can do woodworking anywhere.
Your shop size certainly seems adequate to me, but bigger is always better - at least up to barn size :-).
40x22 would be about right. your current size is a small 2 car garage, (I don't count the portion of the garage for the washer, drier, heater, and basic cabinet area) which requires moving stuff around with fewer items than what you mentioned.
I have about twice the machines in a 16 x 24 dedicated shop. Have been getting along with that for 15 years.
However, planning a move and the next shop will be 25' x 40'. SWMBO and I have struck a deal now that the kids are gone. New house will be 1/3 smaller. Shop will be triple in size. Seems only fair.
On Fri, 5 Aug 2005 10:58:16 -0500, "Steve DeMars"
Now, I agree with that. But that's not necessarily a bad thing. Being pressed for space kind of motivates you to get things finished LOL.
I already have a set of bookshelves in one corner of the basement that are only about 6 hours plus finishing from being done, but I keep starting other stuff. But I will finish them after this current project is done.
While it's not always efficient working in a small shop, where there's a will, there's a way.
You're kidding, right? I have about the same equipment and tools crammed into a 12 X 16 space in a basement, and a steam boiler, water heater, and an old double laundry tub are in the same space, too!
Small ambient air cleaners - which one?
Electrical service - welder outlet work for tablesaw?


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HomeCrimeHomeless woman arrested for burglary, criminal mischief, and drug possession
Homeless woman arrested for burglary, criminal mischief, and drug possession
GAINESVILLE, Fla. – Peggy Elerby, 36, whose address is listed as GRACE Marketplace, was arrested late last night after allegedly entering the victim's apartment uninvited, then refusing to leave; she then allegedly broke the victim's glasses.
The victim reportedly told a Gainesville Police Officer that he and Elerby do not know each other, but Elerby entered his unlocked apartment uninvited and started asking him to give her money. She allegedly refused to leave after he asked her to leave, then threw his prescription glasses on the floor and stomped on them, breaking the glasses.
A neighbor of the victim was in the apartment at the time and told the officer that she saw Elerby enter the apartment, that she refused to leave after being asked, and that she broke the victim's glasses.
Elerby was eventually found hiding in a closet at Windsor Terrace Apartments, reportedly with the permission of the apartment's occupant.
Post Miranda, Elerby reportedly said she was never in the victim's home, but officers saw her in the area of the victim's apartment before she was found and arrested. On the way to the jail, she reportedly said that the victim owes her money.
In a search incident to arrest, officers reportedly found two glass pipes with residue on them, and Elerby reportedly admitted that she uses them to smoke marijuana and synthetic spices. She also reportedly said that she hasn't slept since December 17 because of the drugs she has been taking.
Elerby was previously arrested in November 2021 for hitting and cutting two other homeless people, but those charges were eventually dropped. She was arrested again in April 2022 for hitting a woman and was also charged with five counts of battery on a law enforcement officer. She remained in jail with bail set at $300,000 while awaiting trial and entered a plea of nolo contendere to two counts of battery on a law enforcement officer in July; she was sentenced to two concurrent terms of 270 days in jail with credit for 90 days served.
Elerby has been charged with burglary of an occupied dwelling, property damage over $200, and possession of drug equipment. She has three felony convictions and an active warrant from Georgia; Judge Susan Miller-Jones set bail at $211,000.
You voted for it, you got it. says:
Another resident of Grace, the crime-ridden place.
There's so much drug activity happening at Grace maybe it's time to treat it as a trap house and arrest anyone funding it. Seize the property, sell it for the proceeds and return the funds to the taxpayers. People know who they are and if you can get past the private Gestapo downtown…
Send her back to Georgia, judge.
marvin h rippner says:
GRACE! Craphole!!!!!!!!

redpajama_set_name: RedPajamaCommonCrawl


text:
Kelly Clarkson Kicks Off Talk Show's New Season With Multiple Chris Martin Duets
The covers included "Since U Been Gone," a Whitney Houston classic and Ginuwine's "Pony."
Kelly Clarkson started the new season of her talk show with not one, but five duets with Coldplay's Chis Martin.
Kicking off the new batch of episodes in the Big Apple, Clarkson paid tribute to the resilience and strength of New Yorkers and the magic of the greatest city in the world in her opening monologue.
"We love New York!" she exclaimed, as she was joined by Kristin Chenoweth and Fat Joe at the top of the Monday's show.
"I'm a proud New Yorker man and our city is just a city of hope and inspiration," said the rapper, "Every time they try to knock us down we always come back up with the help of all of America. Cause whenever something happens here in New York, we feel everybody across the whole country feel it with us and we'll keep fighting back."
"This city has always come together and bounced back," added Clarkson, "even in the worst of times, the toughest of times, your spirit is exactly what we all need right now. Sure we thought the pandemic would be over but it's not, so that's cool but we're in the city that never quits!"
As the episode continued, Clarkson proved her duets never quit either — as she and the Coldplay front man performed a number of songs together.
First, the duo performed an acoustic version of Kelly's favorite Coldplay song, "Green Eyes," before the two did a stripped down version of "Since U Been Gone."
While talking about their shared love of Whitney Houston, the two then shared a slowed down rendition of "I Want To Dance With Somebody."
As the two then broke down some of the craziest covers they've ever performed — which, between them, is a lot! — Clarkson recalled trying and failing to do Eminem justice. Eventually, they decided to tackle Ginuwine's "Pony," apologizing ahead of time.
Clarkson also admitted to the Coldplay singer that she had been guilty of singing the band's chart topper "Yellow" more than Martin as it was her daughter's favorite song.
In a heartwarming moment, Kelly invited her children River, 7, and Remy, 5, onstage to sing their favorite song with Chris Martin. Remy, the five-year-old, unexpectedly ended up stealing the show when he interrupted the sing-a-long for a much needed bathroom break!
Next: Giorgio Armani Cool Plum (61) Neo Nude Melting Color Balm Review & Swatches
Next: 54 Hilarious Tweet Reactions To The 2021 Met Gala
Rachel Dratch & Ana Gasteyer Team Up for Holiday Parody "A Clüsterfünke Christmas," Vella Lovell Stars
Dior Birds of a Feather Collection for Fall 2021
Jason Kidd's coaching style described as 'psychological warfare' in Giannis Antetokounmpo's biography
Nicki Minaj & Wendy Williams Seemingly Squash Beef After Wendy Referred To Nicki As An 'Icon' (Video)


Endogenous retroviruses (ERVs) are remnants of ancient retroviral infections that have invaded the germ line of both humans and non-human primates. Most ERVs are functionally crippled by deletions, mutations, and hypermethylation, leading to the view that they are inert genomic fossils. However, some ERVs can produce mRNA transcripts, functional viral proteins, and even non-infectious virus particles during certain developmental and pathological processes. While there have been reports of ERV-specific immunity associated with ERV activity in humans, adaptive immune responses to ERV-encoded gene products remain poorly defined and have not been investigated in the physiologically relevant non-human primate model of human disease.
Here, we identified the rhesus macaque equivalent of the biologically active human ERV-K (HML-2), simian ERV-K (SERV-K1), which retains intact open reading frames for both Gag and Env on chromosome 12 in the macaque genome. From macaque cells we isolated a spliced mRNA product encoding SERV-K1 Env, which possesses all the structural features of a canonical, functional retroviral Envelope protein. Furthermore, we identified rare, but robust T cell responses as well as frequent antibody responses targeting SERV-K1 Env in rhesus macaques.
These data demonstrate that SERV-K1 retains biological activity sufficient to induce cellular and humoral immune responses in rhesus macaques. As ERV-K is the youngest and most active ERV family in the human genome, the identification and characterization of the simian orthologue in rhesus macaques provides a highly relevant animal model in which to study the role of ERV-K in developmental and disease states.
Endogenous retroviruses (ERVs) comprise approximately 8 % of the human genome. While the vast majority of ERVs no longer possess coding capacity for the production of viral mRNAs and proteins, some ERV open reading frames (ORFs) remain intact [1, 2]. Human ERV (HERV) activity is normally repressed in healthy tissues, however, activation of HERV ORF transcription and protein production is detected in numerous developmental and pathological contexts, including embryogenesis, pregnancy, neoplasia, autoimmunity, neurodegeneration, and viral infection [3–10]. In addition, HERV-specific cellular and humoral immunity has been reported in humans, particularly in the context of exogenous retroviral infection with human immunodeficiency virus (HIV) [10–13]. However, ERV activity and ERV-specific immune responses remain poorly defined in the physiologically relevant rhesus macaque model frequently used to study human reproduction, development, neurology, and infectious disease. Therefore, we investigated here if rhesus macaques harbor a functional equivalent of HERV-K (HML-2).
To assess whether a biologically active ERV might exist in the genome of Indian rhesus macaques, we focused on HERV-K (HML-2), the youngest and most active of all ERVs in the human genome . To this end, we used consensus HERV-K sequences to scan the rhesus macaque genome, where we identified three proviral insertion sites on chromosomes 5, 11, and 12. The proviral insertion on chromosome 12 exhibited the greatest sequence homology to HERV-K, with 89 % nucleotide homology to HERV-K Gag, Pro, Pol, and Env open reading frames; we termed this provirus simian ERV-K1 (SERV-K1) (Additional file 1: Figure S1). While the Pro and Pol reading frames contained frameshift mutations leading to premature stop codons, the Gag and Env ORFs remained intact with the potential to code for full length proteins (Fig. 1a). To assess whether the SERV-K1 provirus could produce protein-coding mRNA transcripts, we used mRNA capture to generate a cDNA library from a macaque in which we detected a large SERV-K1 Env T cell response (r02120, see below). The cDNA library from r02120 contained a spliced mRNA transcript coding for the SERV-K1 Env protein possessing canonical structural features of a retroviral Env protein, including a signal leader peptide (L), a RX(K/R)R consensus cleavage site separating the surface (SU) and transmembrane (TM) envelope subunits, a fusion domain, an immunosuppressive domain, and a transmembrane anchor domain (Fig. 1b, c; Additional file 2: Figure S2). Phylogenetic analysis confirmed that SERV-K1 is part of a simian 'sister' lineage of the HERV-K (HML-2) lineage that expanded in the hominid germline (Fig. 1d; Additional file 3: Figure S3). As HERV-K HML2 Env and SERV-K1 Env share >90 % sequence homology, it is likely that, as described for HERV-K Env in humans , SERV-K1 Env retains functional activity in rhesus macaques, and thus may serve as a target for immune responses.
Because SERV-K1 Env retained the ability to code for functional protein, we next assessed whether this activity in rhesus macaques was sufficient to trigger SERV-K1 Env-specific immune responses. To this end, we generated a set of overlapping 15-mer peptides spanning the SERV-K1 Env protein sequence and performed IFN-γ ELISPOT using PBMC from both SIV-infected and -uninfected rhesus macaques. We selected SIV-infected macaques due to the well-documented association between HIV-1 infection and increased HERV activity . Although we screened 29 chronically SIV-infected rhesus macaques (16 progressors and 13 elite controllers), 4 SIVΔNef vaccinated rhesus macaques, and 26 SIV-naïve rhesus macaques we identified only one rhesus macaque mounting T cell responses against SERV-K1 Env (Fig. 2a). As mentioned above, this SIV-infected progressor, r02120, was the same macaque from which we had captured the SERV-K1 Env-encoding mRNA, providing further evidence that SERV-K1 was transcriptionally active in this animal.
Although SERV-K1 Env-specific T cells were only rarely detected in our cohort of macaques via IFN-γ ELISPOT, we identified one response in r02120 targeting SERV-K1 Env526–540 LL15 with surprisingly high magnitude (Fig. 2b). Given that impurities in commercially prepared peptides can result in false positive responses in IFN-γ ELISPOT [16, 17], we confirmed this high magnitude T cell response using SERV-K1 Env526–540 LL15 peptide synthesized by three independent sources (data not shown). Intracellular cytokine staining (ICS) revealed that this high frequency response was CD4+ T cell mediated (Fig. 2c). Strikingly, the SERV-K1 Env526–540 LL15-specific CD4+ T cell response was larger in magnitude than the entire SIVmac239-specific CD4+ T cell response in r02120 (Fig. 2d), and could be detected in ELISPOT at concentrations of 1 μM (Additional file 4: Figure S4). Further analysis of this unusual CD4+ T cell response revealed that SERV-K1 Env526–540 LL15-specific CD4+ T cells were uniformly effector memory in character (Fig. 2e), suggesting continual exposure to low levels of antigen. SERV-K1 Env526–540 LL15-specific CD4 + T cells were also highly polyfunctional, as evidenced by secretion of cytokines (TNF-α and IFN-γ) and chemokine (MIP-1β) and degranulation (measured by CD107a) (Fig. 2f). The effector memory phenotype coupled with the high frequency of responding T cells is reminiscent of the phenomenon of "memory inflation", which is seen with chronic pathogens such as herpes viruses .
In addition to the large SERV-K1 Env526–540 LL15-specific CD4+ T cell response, r02120 also mounted a CD8+ T cell response to SERV-K1 Env108–115 PA8 (Fig. 3a). Similar to the CD4+ T cell response, SERV-K1 Env108–115 PA8-specific CD8+ T cells could be detected in IFN-γ ELISPOT at concentrations of 100 nM (Additional file 4: Figure S4) and were uniformly effector memory in phenotype (Fig. 3b). Thus, while SERV-K1 Env-directed T cell responses are not frequent in SIV-infected rhesus macaques, rare, high frequency, responses can be detected, suggesting that SERV-K1 is active in at least a subset of these animals.
As we had captured an mRNA encoding SERV-K1 Env with all the structural elements of a functional envelope protein, and detected both CD8+ and CD4+ T cells targeting this protein in r02120, we asked whether SERV-K1 Env-specific antibody responses would be present in this animal. To assess this, and to identify the immunogenic domains of SERV-K1 Env, we performed a peptide-based ELISA assay to screen for antibodies directed to SIVmac239 Env and SERV-K1 Env in rhesus macaque r02120. As anticipated, we detected robust antibody responses to SIVmac239 Env (Fig. 4a). However, we also observed robust antibody responses against SERV-K1 Env in the serum of this macaque (Fig. 4b). As the sequence homology between these two envelopes is low (~19 % amino acid homology), the SERV-K1 Env-specific antibodies detected are unlikely the result of cross-reactive SIVmac239 Env-specific antibodies. Furthermore, we found similar antibody targeting of SERV-K1 Env in 3 additional SIV-infected and 2 SIV-naïve rhesus macaques (Fig. 4b). Interestingly, one of the highly targeted regions in this macaque, SERV-K1 Env541–555 TM, is fully conserved with HERV-K Env and a major humoral target in HIV-infected humans . Focusing on six frequently targeted SERV-K1 15-mer peptides, we screened an additional 21 SIV-naïve rhesus macaques, and found consistent antibody targeting of these six SERV-K1 15-mer peptides in all animals tested (Fig. 4c). These data demonstrate that the observed SERV-K1 Env antibody responses represent genuine targeting of SERV-K1 Env and not cross-reactive SIV-specific antibody responses. Thus, SERV-K1 Env protein expression is sufficient to commonly trigger SERV-K1 Env-directed antibody responses in rhesus macaques.
Previous studies reported that infection with viruses such as HSV-1, HIV-1, and cytomegalovirus resulted in an increase in ERV expression in human cells in vitro [19, 20]. To investigate whether the viral infection history of r02120 accounted for its ability to mount SERV-K1 Env-specific T cell responses, we screened a cohort of 24 rhesus macaques, including r02120, for previous viral infections by plasma serology. The pattern of antibody detection against various retroviruses (Simian T lymphotropic virus, Simian retrovirus types one and five, Simian foamy virus), herpes viruses (Herpes B virus, Cytomegalovirus, Lymphocryptovirus, Rhesus rhadinovirus), and paramyxoviruses (Measles virus) did not reveal any correlation between previous viral infections and the presence of SERV-K1-specific immune responses (Fig. 5a). In order to specifically examine whether the observed T cell recognition of SERV-K1 Env LL15 and PA8 in r02120 was the result of cross-reactive SIV-specific T cell responses, we attempted to align these SERV-K1 Env epitopes to SIVmac239 Env and did not find any SIVmac239 Env peptides with greater than 33 and 38 % identity to SERV-K1 LL15 and PA8, respectively (Additional file 5: Figure S5). Furthermore, longitudinal analysis of both the SERV-K1 Env526–540 LL15-specific CD4+ T cell response and Env109–115 PA8-specific CD8+ T cell response in r02120 revealed that these responses were present prior to SIV infection, in contrast to a Vif97–104 WY8-specific CD8+ T cell response that only arose following SIV infection (Fig. 5b). Thus, the LL15- and PA8-specific T cell responses in r02120 likely represent genuine targeting of SERV-K1 Env and not cross-reactive SIV-specific T cell responses. This is in line with a previous report indicating lack of SIV-induced up-regulation of SERV activity in the cells of rhesus macaques . Thus, viral infection status alone does not predict SERV-K1-specific T cell immunity.
In conclusion, these data suggest that, although ERVs have become endogenous self-antigens, ERV activity can generate specific immune responses. The mechanisms by which this ERV-specific immunity is induced and how tolerance to ERV antigens, if any, is overcome, require further investigation. However, these data contribute to the growing body of evidence that ERVs are not inert genomic fossils, but rather represent dynamic protein-coding products that impact developmental, pathological, and immunological processes.
A total of 82 purpose-bred male or female rhesus macaques (RM) (Macaca mulatta) of Indian genetic background were used in this study including 34 SIV-infected RM, 46 SIV-naïve RM, and 2 RM with time points taken before and after SIV infection. Rhesus macaques were housed at the Wisconsin National Primate Research Center, New England Primate Research Center, or the Oregon National Primate Research Center. All protocols were approved by the respective Institutional Animal Care and Use Committees, under the standards of the US National Institutes of Health Guide for the Care and Use of Laboratory Animals.
We used HERV-K consensus sequences  to search the rhesus macaque genome for the simian orthologue of ERV-K. Sequence analysis was conducted using MacVector (Cary, NC, USA) and Geneious (Auckland, New Zealand) software.
Several micrograms of mRNA were purified from BLCL utilizing the Qiagen (Valencia, CA, USA) RNeasy kit and Oligotex RNA kit according to the manufacturer's protocols. cDNA libraries were made from this mRNA with the Invitrogen (Carlsbad, CA, USA) Superscript II cDNA synthesis kit according to the manufacturer's protocol. The cDNA was ligated into the pCMVsport vector and transformed into stbl4 electromax cells from Invitrogen (Carlsbad, CA, USA). Greater than 2 × 105 colonies were plated out from the transformation and were physically scrapped off the plates to make a plasmid prep of the cDNA library. Probes of approximately 50nt in length were designed based on conserved sequences found in an alignment of multiple known ERV. The following probes were ordered from IDT (Coralville, IA, USA) as 5′ biotinylated probes that had been HPLC purified: 5′CAGCTAYGGCTGCTGTAGCAGGAGTTGCATTGCACTCTTCTGTTCAGTC3′ (envelope sequence), 5′GCTGCCAATCCTCCAGTTAACATAGATGCAGATCAACTATTAGGAATAG3′ (gag sequence), and 5′CACTATTATTAACATATACTTCAATAGGATTATCCATCCATGTAACTGCC3′ (envelope sequence). The cDNA library was enriched for the target molecules utilizing the biotinylated probes and the RecA affinity capture method of Zhumabayeva et al. [24, 25]. Specifically, the buffer described by Zhumabayeva et al. was mixed with aqueous Adenosine 5′-[γ-thiotriphosphate tetralithium salt (stored at −80 in small aliquots in a 2:1 ratio with ATP) from Sigma-Aldrich (St. Louis, MO, USA) and 50 ng of probe and 2 µg of RecA protein in a 30 µl reaction volume. This was incubated at 37° for 15 min. Then 5 µg of cDNA library was added and a further 37° incubation of 20 min was done. The RecA was then inactivated by adding 10 % SDS and Proteinase K as described by Zhumabayeva et al. The Proteinase K was incubated for 10 min at 37°. Finally, the Proteinase K was inactivated by adding 1 µl of 100 mM PMSF. Once this was thoroughly mixed, 20 µl of M-270 Dynal paramagnetic streptavidin beads from Invitrogen (Carlsbad, CA, USA) that were previously washed twice with a binding buffer consisting of 10 mM TrisHCL, 1 M NaCl, and 1 mM EDTA were added to the reaction. This was gently mixed by hand for 30 min at room temperature. The beads were washed three times with 10 mM TrisHCl, 2 M NaCl, and 1 mM EDTA wash buffer to remove unbound plasmid. A last wash was done with pure water at 37°. The beads were resuspended in 50 µl of TE buffer and extracted with an equal volume of phenol:chloroform:isoamyl alcohol. The aqueous phase was ethanol precipitated and resuspended in 10 µl of TE of which 0.5 µl was used for heat shock transformation of stbl3 cells from Invitrogen (Carlsbad, CA, USA). The resulting colonies were plasmid purified and Sanger sequenced to screen for the target sequences. The resulting clones are typically enriched for target molecule to better than 50 %, although the percentages can vary widely. The non-target molecules are generally random in their nature and probably represent non-specific sticking to the streptavidin beads. Sequence analysis was conducted using MacVector (Cary, NC, USA) and Geneious (Auckland, New Zealand) software. Accesssion numbers for captured SERV-K1 Env mRNA are KU363810 and KU363811.
ELISPOT screening was carried out as previous described  using 15-mer peptides (with 11 amino acid overlap) spanning the entire SERV-K1 Env open reading frame or the entire SIVmac239 proteome. Intracellular cytokine staining assays were carried out as previously described [21, 26]. Positive responses shown were confirmed using peptides synthesized by three independent sources.
Heat-inactivated serum was diluted 1:1000 and used in a peptide-based ELISA assay, as previously described , against overlapping 15-mer peptides (with 11 amino acid overlap) spanning either the entire SERV-K1 Env open reading frame or the entire SIVmac239 Env open reading frame. ELISA was conducted in duplicate, using absorbance at 450 nm to determine binding antibodies.
Intuitive Biosciences performed serology assays. Individual serum samples were screened for presence of antibodies to a panel of viral pathogens using CSA: Simian Expanded Array and CSA: Simian Detection Kit (Intuitive Biosciences, Madison WI), following the standard manufacturer's protocol. Briefly, samples were diluted 1:100 in the supplied Sample Dilution Buffer and incubated on the CSA: Simian Expanded Array for 1 hour at room temperature. Each sample well was washed five times with Wash Buffer, and a 1:5000 dilution of anti-Simian IgG in Sample Dilution Buffer was added to each well and incubated at room temperature for 1 h. After washing fives times with Wash Buffer, a 1:100 dilution of gold conjugate reagent in gold conjugated diluent was added to each well and incubated at room temperature for 45 min. After five repeat washes with Wash Buffer, each well was rinsed using 1× rinse buffer. SilverQuant reagent A and B were quickly mixed and added to each well, incubating for 3 min while protected from light. Each sample well was quickly rinsed with ultrapure water several times, and the arrays dried with nitrogen gas at approximately 80 psi. Each array was scanned and analyzed using the AthenaQuant System (Intuitive Biosciences). A report was generated with intensity of each spot recorded by antigen as a mean of five replicate spots, in relative intensity units. Cut off values as specified by the manufacturer were used to determine positive and negative designations for each sample. When a sample generates significant intensity of signal on the array, this indicates that the animal is seropositive to the virus represented by the antigen on the array. This does not detect presence of virus, merely presence of specific IgG to the virus, which is indicative of previous exposure or latent and/or subclinical infection.
HLW performed studies and wrote the manuscript. EJL, LTW, FAN, MBW, LPN, PAC, RPJ, RJG, and BRJ helped perform the studies. BRJ and JBS conceived the study. JBS helped perform the studies and helped write the manuscript. All authors read and approved the final manuscript.
This work was made possible by National Institutes of Health grants R21 AI087474, the Office of Research Infrastructure Programs P51 OD011092 and P51 OD011103, and the Bill and Melinda Gates Foundation grant 01526000084 to JBS. We thank Jackie Gillis for expert technical assistance and members of the Wisconsin, New England, and Oregon Primate Research Center Primate Medicine staff for their expert animal care.
12977_2016_238_MOESM1_ESM.pdf Additional file 1: Figure S1. SERV-K1 proviral sequence. Nucleotide sequence of SERV-K1 provirus from chromosome 12 of rhesus macaque r02120 is shown. Long terminal repeat sequences (LTRs) as well as coding sequences for Gag, Pro, Pol, and Env open reading frames are annotated. Note Pro and Pol ORFs contain frameshift mutations resulting in premature stop codons, while Gag and Env ORFs appear intact. RF = reading frame.
12977_2016_238_MOESM2_ESM.pdf Additional file 2: Figure S2. Alignment of captured SERV-K1 Env mRNA to SERV-K1 provirus. Protein alignment of the predicted amino acid sequence of chromosome 12 provirus and two mRNA variants captured from rhesus macaque r02120 is shown. Matches at each amino acid position are indicated with asterisks (*), while mismatches are indicated with periods (.). Mismatches in each sequence are highlighted in yellow.
12977_2016_238_MOESM3_ESM.pdf Additional file 3: Figure S3. Alignment of SERV-K to primate ERVs. Nucleotide alignment showing part of the pol locus of SERV-K from rhesus macaque r02120 aligned to various ERVs from hominids (humans, chimpanzees) and old world monkeys. Matches at each amino acid position are indicated with asterisks (*). The SERV-K1 locus described here is highlighted in grey. CERV = chimpanzee endogenous retrovirus; HERV = human endogenous retrovirus. Sequences shown here were used to construct phylogram in Fig. 1d.
12977_2016_238_MOESM4_ESM.pdf Additional file 4: Figure S4. ELISPOT titration of SERV-K1 Env-specific T cell responses. a Titration of SERV-K1 Env LL15-specific CD4+ T cell response in r02120 (1 week prior to SIV infection) using decreasing concentrations of peptide. Influenza nucleoprotein 15-mer MQ15 is included as a negative control. b Titration of SERV-K1 Env PA8-specific CD8+ T cell response in r02120 (20 weeks post-SIV infection) using decreasing concentrations of peptide.
12977_2016_238_MOESM5_ESM.pdf Additional file 5: Figure S5. Alignments of SERV-K1 Env T cell epitopes to SIVmac239 Env peptides. a Amino acid alignment of r02120 CD4+ T cell epitope SERV-K1 Env LL15 to SIVmac239 Env LL15. This is the only SIVmac239 Env peptide with 10 or less mismatches to SERV-K1 Env LL15. b Amino acid alignment of r02120 CD8+ T cell epitope SERV-K1 Env PA8 to SIVmac239 Env PC8 and AQ8. These are the only SIVmac239 Env peptides with 5 or less mismatches to SERV-K1 Env PA8. For both a and b, matches to the SERV-K1 Env peptide are shown as dashes.


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Marian Foik (Polonia, 6 de octubre de 1933-20 de mayo de 2005) fue un atleta polaco, especializado en la prueba de 4 x 100 m en la que llegó a ser subcampeón olímpico en 1964.

Carrera deportiva
En los JJ. OO. de Tokio 1964 ganó la medalla de plata en los relevos 4 x 100 metros, con un tiempo de 39.3 segundos, llegando a meta tras Estados Unidos que con 39.0 segundos batió el récord del mundo, y por delante de Francia, siendo sus compañeros de equipo: Wiesław Maniak, Andrzej Zielinski y Marian Dudziak.

Referencias

Atletas de Polonia
Medallistas olímpicos de atletismo
Nacidos en Ruda Śląska
Fallecidos en Varsovia

redpajama_set_name: RedPajamaWikipedia


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Published in IJCP December 2021
Pharmacists' Role in Reducing Pharmaceutical Waste
December 10, 2021 | Rashmi Zalpuri, Vishal Kamra, Jk Sharma, Astha Zalpuri
The disposal of leftover/unused medicines is an area of concern in several countries as their improper disposal can impact the environment, which in turn, will affect the health of humans and animals. The main objective of this study is to know the opinion of pharmacists related to the disposal methodologies of unused medications. Several pharmacists were studied in Northern India to evaluate the opinion and the practice towards safe disposal of unused/unwanted medicines. Random interview studies were carried out in North India using a questionnaire. The questionnaire was based on reason, i.e., unused medicine disposal practices opinion of pharmacist as appropriateness of the disposal methodology and awareness of hazard effects of improper disposal. The data was collected from various categories of individuals and interpreted. A total of 135 registered pharmacists participated in this study. The main method that was adopted by the respondents was to dispose the unwanted medications in trash and flush down the sink, which accounted for nearly 42% and 11% of the respondents, and 6% were in the practice of burning/throwing in household waste. Fifty-three percent agreed that they were not aware of the environmental hazards due to this practice and 11% said community take-back programs could help to solve the problem. About 30% of the pharmacists accepted that their pharmacy has collecting point of unwanted medicines. Regulatory authorities should implement and execute policies for proper and safe disposal of unused medicines.
Keywords: Unwanted medications, unused medications, disposal of unused medicines, expired medicines, pharmaceutical waste
The disposal of unused/unwanted medicines from household and healthcare sector is becoming an emerging problem for local and national health and environmental departments. The concerns regarding inappropriate medicine disposal by throwing them in dustbin, flushing down the sink and disposing without proper precautions have been growing and now they are receiving attention.1 Expired, unused, damaged and contaminated substances from households and healthcare activities all come under the category of unwanted medicines. So, it is not uncommon to find them, but whenever such situations arise, there should be clear guidance and methods on how to dispose them. There have been several ways, such as educational materials and regulations, which are available for the same, but the extent to which they are implemented is not clear.2 Not just one, but many problems have been encountered regarding improper disposal of medicines. Some of them are not just limited to humans but animals also.
Keeping unused medicines at home may end up in accidental poisoning in children.3 Pharmaceuticals have a chance of entering drinking water as well and they may affect populations like pregnant women, which emphasizes the need for awareness and practices for safe disposal.4
The fate of the improperly disposed medicines in water is unknown because unfortunately, current water treatment systems do not remove many pharmaceuticals from drinking water.5 Usually the concentration of these medications is negligible; however, long-term exposure to even low levels of multiple medications could be hazardous.6-8
If medicine disposal is improper, the health of the exposed population is at risk. The drains often percolate to the soil, which in turn, contaminates the mass of land and it passes to the food chain. Ineffective treatment of wastewater pollutes freshwater, thus polluting the environment.9 Many types of chemicals were found in 80% sampling of 139 streams of US and the most common were detergents, hormones, prescription/nonprescription drugs, pesticides and antibiotics.10 Some pharmaceuticals have been found in cow, goat and human milk.11
It is a common myth that the expired or unused medicines may be resupplied/repacked by changing their expiry period. While there is no evidence, many people continue to have this opinion.
To cope up with the current market, the increase in manufacturing of pharmaceuticals has led to increase in pharmaceutical waste. The recommendation comes from the World Health Organization (WHO) that unusable medicines should be considered as a pharmaceutical waste, which need to be disposed appropriately, as unsafe disposal of these unwanted or expired drugs often creates a major problem.12 Some medicines should be crushed and mixed with cat litter or coffee grounds and disposed in trash in a leak proof sealed container, which will eventually decrease the chances of poisoning.13-15
However, several other safer methods are also used, such as reverse distribution and drug take-back programs, in which unused/left out medicines are collected by nearby local pharmacies or government/private agencies. In many countries, it has been found to be the best method. The main aim of this medicine collection program is to avoid the unwanted medicine pollution, thus reducing the amount of drugs available for accidental poisoning or theft. Further, such programs provide full support to the public to return unwanted medicines so that they can be disposed of safely.16 Medicines take-back programs that collect medicines from a central location for safe disposal are the most environmentally safe disposal method.17
Considering the fact that pharmacists can have a role in safe disposal of medicines, we conducted a study among pharmacists to determine their awareness, behavior, opinion and practice towards safe disposal of unwanted medicines.
North India (mainly Delhi and NCR) was selected for the study. Pharmacists were selected at random and the study was conducted from July to November 2019. All the selected pharmacists were visited with a questionnaire.
The questionnaire was prepared in English language. Before the survey was conducted, its purpose was well informed to the participants and they were assured that their information would remain confidential, and that only the researchers would have access to the information. For the conclusion and completion of the study, few points were taken care of:
Socio-demographic characteristic of the respondent (male/female), including their age, marital status, educational qualification (Table 1).
Reason mentioned for having unused medicine with consumers including subsiding condition, carelessness, forgetfulness, excess purchase, intolerable side effects, treatment changed, unpleasant taste, expiry date.
Unused medicine disposal practices, including throwing them in waste bins, flushing into toilet, burning, return to pharmacy, buried in ground, given to needy persons.
By using appropriate statistics, the collected data was analyzed.
Table 1. Socio-demographic Data
No. of participants
Graduate in Pharmacy
Diploma in Pharmacy
A total of 135 government and private pharmacists participated in the study and had work experience of 10 to 30 years. Male participants were more than female participants.
Figure 1 represents the participants' opinion on drug take-back. About 30% of the respondents accepted take-back of unwanted medicines and agreed to keep their pharmacy as a drug take-back center while 60% of the respondents did not agree. About 10% didn't answer.
Figure 1. Participants' opinion on drug take-back.
Figure 2 shows participant's opinion and practice towards disposal of medicines. Among these, 42% of the respondents stated that they were throwing the unwanted/left out medicines into trash, 11% were flushing it down the sink/toilet, 30% stated giving back to pharmacy was the best option, 11% said community take-back programs could help to solve the problem and 6% were following other methods like household waste or burning the unwanted pharmaceuticals.
Figure 2. Participants' opinion and practice towards disposal of medicines.
Figure 3 represents the knowledge and awareness of safe disposal methods and hazards of improper disposal. Among the respondents, 53% stated that they were not aware of the consequences due to improper disposal of medicines on humans and environment, 45% were aware of the hazardous effects while 2% didn't answer.
Figure 3. Participants' knowledge and awareness of safe disposal methods and hazards of improper disposal.
Figure 4 represents receipt of instruction or guidelines for the safe disposal methods. Fifty-four percent of the participants said that they couldn't get any guidelines regarding the safe disposal procedures while 46% were getting guidelines for safe disposal from different sources, including 20% from general body meeting, 10% from textbooks and 16% from journals. It was also stated by the participants that national and local government agencies, social media, and drug manufacturing companies have to take a step forward in creating awareness and giving guidelines for environment friendly disposal methods. Most of the participants expressed that there is much less awareness of the issue.
Figure 4. Receipt of instruction or guidelines for safe disposal.
There is high occurrence of pharmaceutical and personal care products in drains, sewerages and rivers and they are signaled to be a future disaster. One of the important effects due to presence of pharmaceuticals in environment is occurrence of antibiotic resistance.
Improper disposal of unused medication is a global problem, both in developed and developing countries. In developing countries, this is more problematic as it often leads to health risks and environmental hazards.
Although there are options for disposing medications, consumers keep the unused leftover as they don't want to let their money go waste, while they don't know the risk of keeping unused medication. The leftover medication at home possesses several risks, such as deterioration of potency, accidental overdose and consumption of expired medicine. Therefore, one has to dispose of these medicines timely, but the disposal shall be done keeping in view the community and environment.
A major hand behind safe disposal of medicines is that of medical professionals since they are directly in contact with the consumers. Pharmacists can influence safe disposal of medicines.
One of the best methods encountered for the safe disposal of unwanted medicines is the drug take-back programs; however, it is less popular, less practiced and leaves people with fewer options. Among the participants in the study, a pharmacist with years of experience said, "We are ready to accept the unused medicines from the patients if they come along with their payment slip and the prescription within couple of months of the purchase of the medicines. Another pharmacist with work experience of years said, "We give back the expired medicines within 3 months back to the distributor/manufacturer, in return we get credits from them, it's a kind of redistribution and an environmentally friendly habit by avoiding unsafe disposal of drugs, especially of antibiotics and cytotoxic drugs.
Usage of medicines from soon to expire stock and avoiding unnecessary prescribing are practices that may contribute to decreased medicinal waste.
This study provided the current scenario of knowledge and practice towards the disposal of medicines by pharmacists. The awareness regarding impact of improper disposal of pharmaceutical waste is still an issue, which needs immediate attention. The current practices are not optimal for collection and disposal for the pharmacist. Pharmacists have the potential to be on the forefront of this movement, but it is essential that their knowledge of proper medication disposal should be complete and accurate.
Teaching of proper disposal of pharmaceuticals in medical, dental, nursing, veterinary or pharmacy curriculum is needed. From this study, it can be said that there is an urgent need for raising awareness and education on medicines take-back program to avoid environmental pollution. There should be national guidelines on the appropriate disposal of unused and expired medicines to minimize the impact on environment and best ways to educate about proper disposal, which may be started through school, religious places, community meetings and at pharmacies.
Begum MM, Rivu SF, Hasan MMA, Nova TT, Rahman MM, Alim MA, et al. Disposal practices of unused and leftover medicines in the households of Dhaka Metropolis. Pharmacy (Basel). 2021;9(2):103.
Bashaar M, Thawani V. Hassali MA, Saleem F. Disposal practices of unused and expired pharmaceuticals among general public in Kabul. BMC Public Health. 2017;17:45.
Labu ZK, Al-Mamun MMA, Harun-or-Rashid M, Sikder K. Knowledge, awareness and disposal practice for unused medications among the students of the private university of Bangladesh. J Biomed Pharm Res. 2013;2(2):26-33.
Collier AC. Pharmaceutical contaminants in potable water: potential concerns for pregnant women and children. EcoHealth. 2007;4(2):164-71.
Stackelberg PE, Furlong ET, Meyer MT, Zaugg SD, Henderson AK, Reissman DB. Persistence of pharmaceutical compounds and other organic wastewater contaminants in a conventional drinking-water-treatment plant. Sci Total Environ. 2004;329(1-3):99-113.
Smith CA. Managing pharmaceutical waste: What pharmacists should know? J Pharm Soc Wiscon. 2002;17:20-2.
Kummerer K. Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources: a review. Chemosphere. 2001;45(6-7):957-69.
Daughton CG. Cradle-to-cradle stewardship of drugs for minimizing their environmental disposition while promoting human health. I. Rationale for and avenues toward a green pharmacy. Environ Health Perspect. 2003;111(5):757-74.
Doerr-MacEwen NA, Haight ME. Expert stakeholders' views on the management of human pharmaceuticals in the environment. Environ Manage. 2006;38(5):853-66.
Buxton HT, Kolpin DW. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams. USGS Fact Sheet FS02702. Reston, VA: U.S. Geological Survey; 2002. Available from: https://toxics.usgs.gov/pubs/FS-027-02/
Azzouz A, Jurado-Sánchez B, Souhail B, Ballesteros E. Simultaneous determination of 20 pharmacologically active substances in cow's milk, goat's milk, and human breast milk by gas chromatography-mass spectrometry. J Agric Food Chem. 2011;59(9):5125-32.
Guidelines for safe disposal of unwanted pharmaceuticals in and after emergencies. WHO, 1999. Available from: https://www.who.int/water_sanitation_health/medicalwaste/unwantpharm.pdf
Where and how to dispose of unused medicines. FDA. Available from: https://www.fda.gov/consumers/consumer-updates/where-and-how-dispose-unused-medicines
Mitka M. FDA: flush certain unused medications. JAMA. 2009;302(19):2082.
Tong AY, Peake BM, Braund R. Disposal practices for unused medications around the world. Environ Int. 2011;37(1):292-8.
Glassmeyer ST, Hinchey EK, Boehme SE, Daughton CG, Ruhoy IS, Conerly O, et al. Disposal practices for unwanted residential medications in the United States. Environ Int. 2009;35(3):566-72.
Yasir AA. Environmental impact of pharmaceuticals and personal care products. J Global Pharm Technol. 2017;09(9):58-64


Kate Hudson may already be a mother to two beautiful boys, but that doesn't mean she's any less excited about her third child—a girl—with boyfriend Danny Fujikawa. Their little one is due any day now, and Hudson and Fujikawa have been dutifully getting their home ready for the baby—in more ways than one. Hudson shared a series of video clips to her Instagram stories on Tuesday, showing how she and Fujikawa have been putting together their baby's nursery—everything from picking out wallpaper to unpacking the bassinet to modeling the baby carrier. In the clips, Hudson can be heard making remarks about the $1,160 Snoo Smart Sleeper the couple selected for their little one, telling Fujikawa, "Oh, look at this. You sort of strap the baby in." In yet another post, Hudson shows Fujikawa putting the finishing touches to the bassinet, which he built himself. "He loves a project," the actress captioned the shot. A final, hilarious post shows father-to-be Fujikawa donning the baby carrier while checking his phone. "I think someone's ready to go," Hudson wrote, along with a number of heart emojis.
Hudson also posted a photo of herself with some friends lounging on her living room couch, feeling her growing baby belly and laughing hysterically. "To get the full value of joy you must have someone to divide it with," she captioned the shot, adding the hashtag #girlfriends. In addition to spending time with her gal pals, Hudson has also been enjoying nights in bed watching TV, as she showed in a post to Instagram last month. "Working all day today and realizing that I should probably be sharing some work life because that's a huge part of my day to day but . . . I'm too pregnant and too lazy so here's a picture of what I wish I was doing all day!" she captioned the shot, which included a photo of her pregnant belly and a glass bowl full of watermelon.
And back in June, Hudson shared a very Zen image of herself lying face up on a yoga mat with her limbs and head supported by pillows. In the photo, she shows off her already large belly in a matching sports bra and leggings set. Early in September, she demonstrated the progress of her baby bump since then in a bathroom selfie in which she dons a colorful brick red kimono with an orange bikini top underneath. "Outie," she captioned the image, in reference to her protruding belly button.


Is the Cloud Secure or Should We Be Wary?
Is the cloud a secure place to store your data or not?
As of right now, less than 10% of the world's data is being stored on the Cloud. Though Cloud storage offers a huge gain in convenience and cost effectiveness, many corporations and other administrative professionals are still very leery. They're just not sure about this Cloud and whether or not their data is going to be safe. We're here to discuss this issue and give you both sides of the story. Let's take a look.
The Cloud is very secure.
A lot of people view the Cloud as being a lot less secure than its traditional counterpart, but the facts just don't back that up. As far as hacking attempts go, both platform types are going to face breaches due to the fact that attacks are often opportunistic in nature. That being said, Cloud services often have highly sophisticated security measures, wheras traditional storage can be outdated and easily infiltrated. Furthermore, some of the world's greatest minds in IT are working on making the Cloud more secure. Not only that, but Cloud Service Providers (CSPs) undergo audits every year to ensure that their datacenters are being monitored and that information about them is being recorded. On-premise systems never see such thorough monitoring.
The Cloud's security isn't perfect.
According to ComputerWorld.com, Google received at least 21,389 requests from the government to reveal personal data. In most cases, Google provided some sort of data in response. Microsoft was asked three times that many times, though they only responded about 2.2% of the time. The fact remains that the government continually makes requests for data. Sometimes that data is given. The security of the Cloud should be examined in this context: This technology is very new and legislation to protect the rights of those who use it usually takes some time to come into effect. In terms of actual security, the Cloud is strong, but that doesn't mean that no one has access to your data.
This entry was posted on Friday, December 2nd, 2016 at 1:22 pm. Both comments and pings are currently closed.


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Computer Questions: How Agp Works
Information about AGP and how it works in simple layman's terms. Explains graphics operations on your PC.
AGP stands for Accelerated Graphics Port and is the architectural standard for the way your PC handles and displays graphics, succeeding PCI (Peripheral Component Interconnect) functions. It was introduced in 1997 by Intel for use in its Pentium II based motherboards but has since become the industry standard for the way graphics is processed on your PC.
AGP delivers graphics performance comparable to what you routinely find on dedicated graphics workstations costing upwards to $20,000, for a fraction of the price. Graphical elements such as texture mapping, z buffering and alpha blending allows for realistic 3D images in games and scientific applications without having to get up off a lot of cash for specialized hardware that couldn't be used for much of anything else.
Now, your eyes probably glazed over at the terms 'texture mapping', 'z buffering' and 'alpha blending'. Well, here is a quick explanation of each and how they work in processing graphics on your video card. Texture mapping is a graphics process that renders a 2 dimensional (2D) image and wraps it around a 3 dimensional object.
Z buffering is an algorithm, or formula, that makes sure perspective works in the virtual world the way it does in the real world. For instance, objects in the foreground blocks those in the back ground. Things such as the display of shadows and line-of-site viewing from the vantage point of the user are also handled as part of the z buffering process.
Alpha blending is the process of determining how each pixel element in an image will behave in terms of opacity versus transparency. Each pixel in 32-bit graphics has four channels: Red, Green, Blue, and an alpha channel, which governs how the previous three in the current pixel look when layered on top of another pixel.
Now how does all this relate to the AGP and your video card? Speed, detail, and frame rate in 3D applications. Frame rate is how many frames can be displayed in a second. This is much faster than the eye can determine image from image, thus giving the illusion of movement and animation when in actuality you are seeing individual snap shots.
PCI based video cards supports a data transfer rate of up to 132 MB/s (megabits per second) in 32 bit graphics, while AGP based cards support up to 533 MB/s. PCI cards must share bandwidth with other peripheral devices in the PC such as the printer port and hard drive controller. It must also preload texture data into video memory. Additionally, PCI cards must perform one instruction at a time. All of this affects graphics performance. Because of the wider bandwidth of data transfer between motherboard processor and your AGP based video card, this blending and texture information is processed on the fly in real time as opposed to being preloaded into your video card's memory and accessed at periodic intervals.
The AGP maintains a higher data transfer rate between video card and motherboard through multiple direct memory queries. It uses your main system memory in a dynamic fashion. That is, memory that is not in use by the graphic card is returned to the operating system on a need to need basis. Because your video card, using the Accelerated Graphics Port, accesses your RAM directly on a wider bandwidth, graphics information is processed faster, resulting in more realistic displays and smoother animations.
Nowadays, AGP slots come standard on motherboards. There is in option in you BIOS setup to allocate a portion of system memory to AGP video functions if the motherboard has an onboard graphics card. The best thing, however, is to purchase an actual AGP graphics card as it will always be better than onboard graphics. Also you need to consider RAM. As AGP functions use actual system RAM, you need to ensure you have enough for that and normal system operation. More memory equals better graphics performance and smoother all around PC operations.
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Home WORLD M400X Skycar – You Can Now Buy This Flying Car on eBay...
M400X Skycar – You Can Now Buy This Flying Car on eBay For $5 Million
This is the original Moller M400 Skycar®. It is the world's first true VTOL (vertical-takeoff-and-landing) "Flying Car" to be demonstrated. The M400 Skycar® is a true museum piece, developed over many years and first flown in 2001 at the company's shareholder meeting. It has since flown (under a restricted environment) many times.
image/text credit: moller
The M400 Skycar® is a true museum piece, developed over many years and first flown in 2001 at the company's shareholder meeting. It has since flown (under a restricted environment) many times.
A collector who buys the M400 will receive it in its original 2001 flight form and condition, complete with eight Rotapower engines that produced over 720 hp allowing the M400 Skycar® to take off and land vertically. It should be noted, however, in this original form, it does not have FAA approval and a condition of this offer is that it cannot be flown.
Nonetheless, this vehicle can be the centerpiece of any car or aircraft collection in a public or private museum. Reassembling the M400 back to its original state will take some time.Alternatively, a bidder can buy the M400 Skycar® and with the support of Moller International, make it the world's first FAA approved VTOL capable "flying car".
Over the last 30 years, Moller International has spent more than $150 million developing the engine and airframe technology to make this prototype possible.
A modified version of the original M400 Skycar® was previously offered for sale, but failed to meet the reserve. The M400 Skycar® is now superseded by a hybrid Skycar® 400 configuration and is available to be converted back to when it first flew.
VIAMoller International
SOURCEmoller


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Discover more about the building by viewing all Sylvan Collection Urban Townhomes condos for sale. In love with the neighbourhood? There are 4 townhouses for sale (Dufferin Grove). Browsing properties outside of Dufferin Grove? The city also has 352 townhouses for sale in Toronto. The list price of $1,399,900 is 0% lower than the average list price of $1,099,315 for Dufferin Grove real estate and 66.01% higher than the average list price of $843,246 for Toronto real estate.
Property Details Home facts for 44 Havelock Street: utilities, exterior, parking etc.
Rooms Room details for 44 Havelock Street: size, flooring, features etc.
There are currently no condos for sale at 44 Havelock Street. Contact us for this building's price history or to see units in nearby buildings.


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In Anglo Irish Bank Corporation Limited v. Quinn Investments Sweden AB & Others (1) the High Court had to consider applications under Articles 23 and 28 of Regulation 44/20001 (the "Brussels Regulation"). Ultimately, whilst it dealt with the application under Article 23, the High Court felt that, on the Article 28 application, further clarification was required and referred the matter to the Court of Justice, adjourning the proceedings pending that determination.
A dispute arose between the parties in relation to the provision of finance to the Quinn Group by the plaintiff, which sought declarations as to the ownership of share pledges. A Swedish company (the first named defendant) had been set up by the Quinn family through which property was owned. That company had various subsidiaries, including Cypriot companies, which held shareholdings in other companies which directly held the properties. The plaintiff had prima facie security over the property portfolio and the companies that held the property. However, the plaintiff alleged a conspiracy to alter the way in which the property portfolio was held, with the object of depriving the plaintiff of its security over the property portfolio. The Quinns applied to stay or dismiss the proceedings under Article 23 on the basis that a related action was pending in Cyprus. They also sought declarations that the courts of Sweden and Cyprus had jurisdiction to settle disputes about the share pledges under Article 28.
At the outset, Clarke J. noted that, notwithstanding the merits of the claim, he was only concerned with the question of jurisdiction. He outlined that, in the EU context, the Brussels Convention and, latterly, the Brussels Regulation, had been adopted to specify the particular jurisdiction or jurisdictions which are appropriate to decide particular disputes. Moreover, as sell as setting out rules which determine the jurisdiction(s) most appropriate to decide on a particular dispute, the Brussels Regulation also identifies which countries' courts are to make a decision as to where the proceedings will be tried. Although those rules are relatively detailed, he noted that the interpretation of those rules can be difficult and the application of the rules to individual cases can be controversial. Inasmuch as possible, it is important that there are clear rules as to which countries' courts are to make the decision as to where the trial is to be held, because without them, there is potential for differing views to be adopted.
It was, Clarke J. felt, important to bear in mind that that there were a number of proceedings in being between the parties in various jurisdictions. He identified that those other cases formed part of the picture against which the jurisdictional issues before him in this case fell to be considered. He noted that, although the legal and factual bases underlying the proceedings brought by the Quinns in Cyprus and Ireland were the same, the security at which each of the sets of proceedings were directed was different. As part of the proceedings before him, the plaintiff sought orders to restrain the Quinns from taking any steps in furtherance of the alleged plan to restructure the property holdings to limit the effectiveness of the plaintiff's security. The Quinns brought their applications under Articles 23 and 28 in respect of those proceedings brought by the plaintiff.
Even before considering those articles, he referred to Bosphorus Hava Yollari Turizm Ve Ticaret Anonim Sirketi v. Minister for Transport (2). There, Murphy J. stated that the teleological or schematic approach was a fundamental principle of interpretation to be applied to EC regulations and directives. The Supreme Court approved such a position in Radio Limerick One Ltd v. Independent Radio and Television Commission (3). Consequently, Clarke J. here opined that the starting point for any consideration of the Brussels Regulation is its recitals, and he cited recitals 2, 11, 12, 15 and 16.
On the Article 23 application, he noted that there was nothing in the relevant pledge agreements which purported to confer exclusive jurisdiction on, respectively, the Swedish or Cypriot Courts. Although those courts did have jurisdiction by reference to the relevant clauses, no provision was made for those courts to have exclusive jurisdiction. Clarke J. found that the sole basis on which Article 23 could exclude the jurisdiction of the Irish Courts (which he found did arise) was if there were relevant and applicable jurisdiction clauses, which conferred an exclusive jurisdiction on the courts of a jurisdiction other than Ireland. Since the clauses in the pledge agreement did not, he held that Article 23 had no bearing on these proceedings.
In this regard, Clarke J. noted that the proper approach for a court to adopt in circumstances where three separate courts are seised of the same or similar issues has only been considered in one case being Masri v. Consolidated Contractors International Company SAL & Ors (5). That case, however, he felt was "unusual" and ultimately offered him no guidance.
In the current context, he stated that it was not the function of the Courts of Ireland to interfere with the proper consideration by the Cypriot Courts as to whether those courts had jurisdiction and he noted that an application had in fact been brought on the basis that they were related to the first in time Irish proceedings. This was a matter for the Cypriot Courts to decide that question and Clarke J. stated that it would be wholly inappropriate for him to offer any view on that determination. Notwithstanding that, the Cypriot Court's ruling on the issue is of some significance since, if the Cypriot Court eschewed jurisdiction, there would be no Cypriot proceedings to prevent the Irish Court in dealing with these proceedings in the ordinary way. Accordingly, Clarke J. refused to decline jurisdiction (by dismissing these proceedings under Article 28) at this stage as to do so would pre-empt the decision of that Court. Ultimately, he felt that, since there was no clear guidance as to what is to happen in the context of a third set of proceedings being brought in a jurisdiction first seised of at least connected proceedings, the best course of action was to obtain definitive guidance by referring the question to the Court of Justice.
In coming to that conclusion, he observed that the approach to adopt where there are "three sets of proceedings pending, two of which (being the first and third in time) are pending in the courts of one member state while the other is pending in the courts of a different member state is not entirely clear." He also identified that it was also necessary and important to decide which courts should make the jurisdictional decisions and to determine which actions are "related" for the purpose of the Brussels Regulation.
Clarke J. declined the application made under Article 23 on the basis that there is no agreement that would confer exclusive jurisdiction on the courts of any other member state. On the propriety of the Irish Court dismissing or staying these proceedings under Article 28, the matter was referred to the Court of Justice. Finally, in circumstances where that issue was to be referred, he adjourned the application before him pending the ruling of the Court of Justice.


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Just four games into the season and already Seattle Sounders FC has trotted out three different formations. Opening Day against Sporting Kansas City it was a 4-4-2. Against Toronto FC it was a 4-2-3-1 that included Clint Dempsey in his only start. During Dempsey's suspension against Montreal and Columbus the club played a 4-3-3. Against the Portland Timbers on Saturday (12 p.m. PT on NBCSN/97.3FM) Dempsey will be back from suspension and U.S. National Team duty.
He is healthy and fit, but there is a simple question yet to be answered: How does Dempsey fit in the 2014 side?
Head Coach Sigi Schmid gave no indication as to whether the successes of the three-man midfield means that it will be back on Saturday. "We'll see how all that fits back together," Schmid told the media after Tuesday's training session.
Seattle's version of the 4-3-3 uses a central triangle (that's typical), with one man at the point (fairly typical), but diverges from most clubs that use this shape in that the player that occupies that top space shifts based on whether Sounders FC is in attack or defense.
That would have to change if Dempsey is inserted centrally in that band of three.
Osvaldo Alonso would obviously sit behind, but in the attack Dempsey needs to be higher than how Gonzalo Pineda plays. In defense, Dempsey is unlikely to be the defensive player that Micheal Azira and Andy Rose were when they started.
That would seem to indicate a shift back to the 4-2-3-1 where the wide midfielder would offer defensive support closer to their own goal. This is most similar to how Dempsey slots into the United States squad, where he is a creative force and a scoring threat taking a little bit of the modern No. 10 and the old trequartista into the band of three.
Both Lamar Neagle and Kenny Cooper showed that they can put in the defensive work while also creating scoring opportunities while in a three-forward set. They could likely do the same in the five-man midfield.
But there is also an alternate path that Schmid could choose. He could stick with that 4-3-3, and instead of having Dempsey in the middle, Schmid can put him wide and left. It is a spot most similar to where he worked with Fulham back when he served the world notice that an American could dominate in the Premier League. The central band provides cover while the forwards act as harriers, disturbing the preferred passes of the opposition.
When Dempsey is out here he is primarily a scorer who cuts in from the wide space to shoot at angles. He also has the options to slip the ball into the central forward, back to a run by a central midfielder or switch to the far post.
If Schmid decides to start Dempsey after his short week of training with the USA, there will be options as to how to use the talented attacker. Schmid likely already knows what he will do, but he's also not tipping his hand – not that he would normally, but certainly not with Portland being the opponent.


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Jamal Ben Saddik (; born October 3, 1990) is a Belgian-Moroccan professional kickboxer. He is a former two-time Glory Heavyweight Championship challenger and 2018 Glory Heavyweight Grand Prix tournament winner.

Combat Press ranked him as a top ten heavyweight between September 2015 and July 2021, peaking at No. 3.

In Glory, Ben Saddik was involved in doping controversies and was removed from the official rankings by the promotion in 2023. In 2015 and 2022 he emerged victorious in the bouts against Anderson Silva and Benjamin Adegbuyi, but were both cut short by Ben Saddik testing positive for banned substances and receiving suspensions, with the fights reversed to a 'no contest'.

Personal life
Ben Saddik was born and raised in Antwerp. His parents emigrated to Belgium from the village of Ait Hadifa in northern Morocco. He was taken to a kickboxing gym at the age of 9 by his father, who feared life in the streets would have a negative impact on his son. As a teen, Jamal was shot and grazed with a bullet after a street fight. In 2012, he was diagnosed with thyroid cancer, which would emerge again around a year later. Ben Saddik has since been cancer-free. He was a student of Cor Hemmers until 2021.

Career

Early years
He made his debut in the It's Showtime promotion on March 6, 2011, at Fightingstars presents: It's Showtime Sporthallen Zuid and beat Rico Verhoeven. He was then scheduled to fight Anderson Silva at Fightclub presents: It's Showtime 2011 on May 21, 2011, but the bout was scrapped when Silva was instead moved to the It's Showtime 2011 Lyon card to fight Daniel Ghiță.

He TKO'd Vitaly Oparin in round one at United Glory 15 on March 23, 2012, and followed this up with a fourth-round KO of Nikolaj Falin on June 2, 2012.

Glory
Ben Saddik lost a decision to Jahfarr Wilnis at Glory 2: Brussels on October 6, 2012.

But, despite this setback, he was invited to compete at Glory 4: Tokyo - 2012 Heavyweight Grand Slam, the biggest heavyweight tournament in kickboxing history, on December 31, 2012. He upset world #4 Errol Zimmerman at the opening stage, taking a decision. In the quarter-finals, he beat Remy Bonjasky on points, but lost via a quick body kick from Daniel Ghiță in the semis. Despite coming back from the tournament empty-handed, Ben Saddik's stock rose greatly by beating two much higher-rated fighters.

Ben Saddik fought Peter Aerts at Glory 8: Tokyo - 2013 65kg Slam on May 3, 2013, in Tokyo. He started well and floored Aerts in round one, but the Dutchman rallied back and dropped him three times in the second, forcing a referee stoppage.

He was knocked out late in round three by Ben Edwards in a slugfest at Glory 12: New York - Lightweight World Championship Tournament in New York City on November 23, 2013.

He was expected to fight Benjamin Adegbuyi at Glory 14: Zagreb in Zagreb, Croatia on March 8, 2014. However he broke his hand while training so he was replaced with Dmytro Bezus.

He knocked out Nicolas Wamba in round two at Glory 16: Denver in Broomfield, Colorado, US on May 3, 2014.

He lost via disqualification when he attacked a downed Hesdy Gerges just seconds into their bout at Qabala Fight Series #1 in Qabala, Azerbaijan on June 29, 2014. Following this incident, Glory suspended him for six months. Ben Saddik would later issue a public apology to both Gerges and fans of the sport.

First suspension and return
Ben Saddik rebounded from his DQ loss to Gerges with a first-round knockout win over Mamoudou Keta, during Glory 22. Ben Saddik won his next outing with Glory as well, defeating Anderson Silva by a third-round TKO. On August 7, 2015, it was announced that Ben Saddik failed a drug test prior to Glory 23: Las Vegas. He was suspended and fined $6,650 by Nevada State Athletic Commission until 8 August 2016. Ben Saddik must also submit clean drugs tests for steroids and diuretics at 30, 10 and 3 days before next fight in Nevada. As a result, his TKO win against Anderson Silva was changed to a no contest.

His next two fights were outside of Glory in the regional circuits. He was first scheduled to fight Brian Douwes during A1 WCC 20. Douwes won the fight by decision. His next fight was against Andre Schmeling during A1 WCC 21.

He also had one with ACB Kickboxing, against Gordon Haupt. Ben Saddik won by a first-round KO.

Returning to Glory, Ben Saddik won decision against Ismael Londt and Guto Inocente.

He was given a chance to fight Rico Verhoeven for the Glory Heavyweight title. Jamal lost to Rico Verhoeven by TKO for the Glory World Championship 49 after his won fight in 2011. He was seen spitting in the face of Rico Verhoeven in a prefight press conference for which he was fined after the incident.

On December 8, 2018, he won the Glory 62 Heavyweight Contender tournament by knocking out Benjamin Adegbuyi in the first round of the final, after winning his fights against Junior Tafa and Guto Inocente in the quarter and semi-final respectively.

He signed a new, long term, contract with Glory in January 2020.

In October 2020, Glory announced Ben Saddik would fight a rubber match with Rico Verhoeven for the Glory Heavyweight title, although they didn't announce a date for the fight. The fight was later scheduled for Glory 77. He withdrew from the bout, 11 days before the event, due to a back injury.

Ben Saddik was scheduled to face Benjamin Adegbuyi at Glory: Collision 3 on October 23, 2021. The two of the previously fought at Glory 62: Rotterdam, in the finals of the 2018 Glory Heavyweight Contender tournament, with Ben Saddik winning by a first-round knockout. Ben Saddik was later rescheduled to face Rico Verhoeven at the same event, as a late replacement for Alistair Overeem, who was forced to withdraw due to an injury. Ben Saddik lost by fourth-round TKO.

Ben Saddik was booked to face the former Enfusion Heavyweight Champion and 2020 Glory 76 Heavyweight Tournament winner Levi Rigters at Glory 80 on March 19, 2022. The bout was cancelled minutes before starting, due to riots breaking out in the crowd.

Second suspension
Ben Saddik faced Benjamin Adegbuyi, in an eliminator for the interim Glory heavyweight championship, at Glory 81: Ben Saddik vs. Adegbuyi 2 on August 20, 2022. He made quick work of his opponent, as he won the fight by a first-round knockout. Ben Saddik knocked Adegubuyi down once prior to the stoppage.

In January 2023, Glory announced Ben Saddik is suspended since his fight with Benjamin Adegbuyi at Glory 81 on 20 August 2022, due to an investigation into a rule violation. It was revealed on February 8, 2023, that he had tested positive for performance enhancing drugs. As a result, his victory over Adegbuyi at Glory 81 was overturned to a no contest. He was also suspended for 15 months, beginning with August 20.

Legal troubles
On March 10, 2021, he was arrested in Antwerp, Belgium in a major operation targeting organised crime in Belgium and the Netherlands. Ben S. was referred to as the seller ('reseller') of the secured crypto devices. His lawyer emphasized though that the arrest of Jamal Ben Saddik has nothing to do with the trade in or possession of prohibited substances such as cocaine (Operation Sky). On March 12, 2021, the Mechelen Council Chamber ordered Ben Saddik to be jailed for 30 days. His attorney immediately filed an appeal. The appeal will be decided in 14 days by the Antwerp Court of Appeal.

Championships and accomplishments
Glory
2018 Glory Heavyweight Grand Prix (+95 kg/209.4 lb) Winner
CombatPress.com
2015 "Comeback of the Year"
2018 "Comeback Fighter of the Year"

Kickboxing record

|-
|- style="background:#c5d2ea;"
| 2022-08-20 || NC ||align=left| Benjamin Adegbuyi || Glory 81: Ben Saddik vs. Adegbuyi 2 || Düsseldorf, Germany || NC (Overturned) || 1 || 2:34
|-
! style=background:white colspan=9 |
|-
|- bgcolor="#FFBBBB"
| 2021-10-23 || Loss ||align=left| Rico Verhoeven || Glory Collision 3  || Arnhem, Netherlands || TKO (Punches) || 4 || 0:56
|-
! style=background:white colspan=9 |
|-
|-  bgcolor="#CCFFCC"
| 2018-12-08 || Win||align=left nowrap| Benjamin Adegbuyi || Glory 62: Rotterdam Final || Rotterdam, Netherlands || KO (Left hook)|| 1 ||  1:52
|-
! style=background:white colspan=9 |
|-
|-  bgcolor="#CCFFCC"
| 2018-12-08 || Win||align=left| Guto Inocente || Glory 62: Rotterdam Semi Final|| Rotterdam, Netherlands || Decision (Unanimous) ||  ||  3:00
|-
|-  bgcolor="#CCFFCC"
| 2018-12-08 || Win||align=left| Junior Tafa || Glory 62: Rotterdam Quarter Final|| Rotterdam, Netherlands || TKO (2 Knockdowns Rule) || 1 ||  2:55
|-
|-  bgcolor="#CCFFCC"
| 2018-09-29 || Win||align=left| D'Angelo Marshall || Glory 59: Amsterdam || Amsterdam, Netherlands || TKO (3 Knockdowns Rule) || 1 ||  0:57
|-
|-  bgcolor="#FFBBBB"
| 2018-05-12 || Loss||align=left| Jahfarr Wilnis || Glory 53: Lille || Lille, France || Decision (Unanimous) || 3 ||  3:00
|-
|-  bgcolor="#FFBBBB"
| 2017-12-10 || Loss ||align=left| Rico Verhoeven || Glory 49: Rotterdam || Rotterdam, Netherlands || TKO (Head kick and Punches) || 5 || 1:10
|-
! style=background:white colspan=9 |
|-
|-  bgcolor="#CCFFCC"
| 2017-03-25 || Win ||align=left| Guto Inocente || Glory 39: Brussels || Brussels, Belgium || Decision (Unanimous) || 3 ||  3:00
|-
|-  bgcolor="#CCFFCC"
| 2016-12-10 || Win ||align=left| Ismael Londt || Glory: Collision || Oberhausen, Germany || Decision (Unanimous) || 3 || 3:00
|-
|-  bgcolor="#CCFFCC"
| 2016-10-16 || Win ||align=left| Gordon Haupt || |ACB KB 8: Only The Braves || Hoofddorp, Netherlands || KO (Right hook) || 1 || 1:24
|-
|-  bgcolor="#CCFFCC"
| 2016-05-28 || Win ||align=left| Andre Schmeling || A1 World Combat Cup 21 || Eindhoven, Netherlands || KO (Right uppercut) || 1 ||
|-
|-  bgcolor="#FFBBBB"
| 2015-11-28 || Loss ||align=left| Brian Douwes || A1 World Combat Cup 20 || Eindhoven, Netherlands || Decision || 3 || 3:00
|-
|-  bgcolor="#c5d2ea"
| 2015-08-07 || NC ||align=left| Anderson Silva || Glory 23: Las Vegas || Las Vegas, Nevada, USA || NC (Overturned) || 3 || 2:55
|-
! style=background:white colspan=9 |
|-
|-  bgcolor="#CCFFCC"
| 2015-06-05 || Win ||align=left| Mamoudou Keta || Glory 22: Lille || Lille, France, || KO (Punches) || 1 || 1:59
|-
|-  bgcolor="#FFBBBB"
| 2014-06-29 || Loss ||align=left| Hesdy Gerges || Qabala Fight Series #1 || Qabala, Azerbaijan || DQ (Attack on a downed opponent) || 1 || 0:20
|-
|-  bgcolor="#CCFFCC"
| 2014-05-03 || Win ||align=left| Nicolas Wamba || Glory 16: Denver || Broomfield, Colorado, USA || TKO (Left Hook) || 2 || 1:24
|-
|-  bgcolor="#FFBBBB"
| 2013-11-23 || Loss ||align=left| Ben Edwards || Glory 12: New York || New York City, New York, USA || KO (Right Hook) || 3 || 2:52
|-  bgcolor="#FFBBBB"
| 2013-05-03 || Loss ||align=left| Peter Aerts || Glory 8: Tokyo || Tokyo, Japan || TKO (Referee Stoppage) || 2 ||
|-  bgcolor="#FFBBBB"
| 2012-12-31 || Loss ||align=left| Daniel Ghiță || Glory 4: Tokyo - Heavyweight Grand Slam, Semi Finals || Saitama, Japan || KO (Liver kick) || 1 || 0:35
|-  bgcolor="#CCFFCC"
| 2012-12-31 || Win ||align=left| Remy Bonjasky || Glory 4: Tokyo - Heavyweight Grand Slam, Quarter Finals || Saitama, Japan || Decision (Unanimous) || 2 || 2:00
|-  bgcolor="#CCFFCC"
| 2012-12-31 || Win ||align=left| Errol Zimmerman || Glory 4: Tokyo - Heavyweight Grand Slam, First 16 || Saitama, Japan || Decision (Unanimous) || 2 || 2:00
|-  bgcolor="#FFBBBB"
| 2012-10-06 || Loss ||align=left| Jahfarr Wilnis || Glory 2: Brussels || Brussels, Belgium || Decision (Unanimous) || 3 || 3:00
|-  bgcolor="#CCFFCC"
| 2012-06-02 || Win ||align=left| Nikolaj Falin ||  || The Netherlands || KO (Punches) || 2 ||
|-  bgcolor="#CCFFCC"
| 2012-03-23 || Win ||align=left| Vitaliy Oparin || United Glory 15 || Moscow, Russia || TKO || 1 ||
|-  bgcolor="#CCFFCC"
| 2011-03-06 || Win ||align=left| Rico Verhoeven || It's Showtime Sporthallen Zuid ||Amsterdam, Netherlands || TKO (Corner Stoppage) || 2 || 1:00
|-
| colspan=9 | Legend:

See also
 List of male kickboxers

References

External links
Profile at GLORY

1990 births
Living people
Sportspeople from Antwerp
Moroccan male kickboxers
Belgian male kickboxers
Heavyweight kickboxers
Glory kickboxers
Doping cases in kickboxing
Moroccan sportspeople in doping cases
Belgian sportspeople in doping cases
Moroccan expatriate sportspeople in the Netherlands
Belgian expatriate sportspeople in the Netherlands


T he Account Status feature lets you create an account or contact status field and control this field based on related objects such as Leads, Contacts or Opportunities. A daily batch can be scheduled to run and update the specified field with the indicated value.
To begin, launch the Account Status feature from the Lane Four Launcher. The first tab lets you create a new formula for Accounts. Click Create New to specify the source object and source field along with a value.
Account Status field lets you determine the desired status of the account. In the Destination API field, paste the field's API name of the account status field.
Similarly, the Contact Status Formulae tab allows you to set a Contact Status based on an Opportunity Stage.
Click here to download a spreadsheet that can help you manage contact status and account status values using the above steps. If you would like to update a contact status based on an Opportunity stage, navigate to the Contact Status Formulae tab and create new.
Enter a number in the rank field to order the rules in this setting. Set the value of the Opportunity Stage that should be considered when changing the contact status, such as "Interest/Discovery". Next enter the value that should show on the contact record such as "Working". The Destination API field should have the developer name of the contact field which shows this value.
Activate it via the IsActive checkbox. It will display in the contact status details once saved.