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St. Frederick hires Dearman as baseball coach
St. Frederick High School principal Blair David is pleased to announce the hiring of Adam Dearman as the new head baseball coach.
Hard working, caring, leader, exceptional coach, and all-around good guy are just a few of the terms many have used to describe Adam. He is Monroe native, a SFHS alumnus, and a graduate of the University of Louisiana Monroe.
"We are excited to be able to hire a St. Frederick former player and graduate," St. Frederick interim athletic director Delton Dearman said. "Adam was an all-state MVP SF baseball player and was an assistant coach at St. Frederick before going to Loyola. He has been left a very good program by former Coach Joey Lee, who left for a head football coaching job, and we feel confident in Adam's ability to take the baseball program to the next level."
Adam Dearman has spent the last 4.5 years as the assistant baseball and football coach at Loyola College Prep in Shreveport, LA, but he is enthusiastic about the opportunity to return to his alma mater.
"I jumped at the opportunity to be able to return to St. Frederick as the head baseball coach," Adam Dearman said. "St. Frederick has always been special to my family, and I am thrilled to be able to lead the baseball program and mold young baseball players into winners. I am also excited to be able to work alongside my dad here at St. Frederick. Lastly, thank you to the school administration for giving me this opportunity."
Adam Dearman is married to the former Anna Giannobile, who is also a graduate of St. Frederick High School, and they have one daughter.
Please contact the St. Frederick High School Athletic Department at (318)-323-9636 if you have any questions or need further information.


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When Someones Comes Into Your Home And Tries To Steal Your Dinner (Hilarious Video)
Sponsored by: www.criaderodecaracoles.com/category/especies
On YouTube, video is viral. A fisherman ended up 'frozen' in fear when he ran into a mysterious creature that emerged from the depths to snatch his newly hunted prey.
Creepy moment on YouTube. An afternoon of fruitful fishing for a man became the worst experience of his life, as he was brutally surprised with the appearance of a strange creature that emerged from the river to snatch the freshly caught fish from a single pull. The final passage of the viral video started messages of admiration to users in the United States, Mexico and Spain.
An afternoon of fruitful fishing for a man was tainted by the untimely force exerted by a mysterious creature against a fishthat fell into the trap of its powerful hook. The fisherman was very surprised with what happened, since it was impossible to remove the animal from the sea.
After several minutes of tension, the fisherman came to observe that inside the ocean, a huge sea creature firmly grasped its prey without releasing it. The viral YouTube video showed the curious moment that the man lived after playing "win" with a mysterious animal that after zooming to the camera would discover his true identity.
Thanks to the characteristics of the strange creature , the boy managed to identify her as a huge sea turtle . As is known, these types of animals are found in oceans, lagoons and bays, where they catch their favorite prey such as fish, mollusks, insects, larvae and crustaceans.
The viral video shared on YouTube showed the incessant dispute in which the fisherman engaged with the sea turtle that did not miss the opportunity to steal his prey. At the end of the clip, the gigantic sea creature won the jackpot and the man regretted his bad luck.
The viral video on YouTube was shared by the ViralHog page, which accumulated more than 3,000 views, 200 shared and dozens of comments. "Once I caught a fish, but a turtle snatched it from me", "Incredible!", "What a terror", the users left on social networks.
Watch the YouTube viral video:
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It's finally over. Phaedra Parks and her estranged husband, Apollo Nida, have reached a divorce settlement, TMZ reports. According to the site, the Real Housewives of Atlanta star and Nida's settlement is confidential, so the terms have yet to be disclosed.
The pair had been battling back and forth over spousal support and properties. The Bravo star, 43, initially filed for divorce from the businessman, 38, in October 2014 after nearly five years of marriage.
TMZ reports that the exes have also reached an agreement regarding custody of their kids Ayden, 7, and Dylan, 4. The exes will share legal custody while Parks will maintain primary physical custody. Nida, who is serving time in prison for money fraud, will get weekly phone calls with them.
The settlement comes after their divorce judgment was reversed by a judge in March and after Nida got engaged to Sherien Almufti, a woman he had been friends with since 2013.


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Home > Fantasy > 2010 > 11/22 > Leafs' starting goalie Giguere hopes to return this weekend
Leafs' starting goalie Giguere hopes to return this weekend
Updated: November 22, 2010 at 7:31 am by Alexander Monaghan
The Leafs play back-to-back games this weekend at Ottawa and Buffalo, in which Giguere hopes to return during. Giguere hopes to begin practicing tomorrow, which would be an earlier return than originally expected. In his absence, Jonas Gustavsson has been excellent going 1-1 and allowing only three goals over 132 minutes.
Giguere will miss one to two weeks. We will keep you updated, for now prepare for the team to roll with Gustavsson.
Jean-Sebastien Giguere left last night's game with tightness in his groin area. The injury is seen more of a precautionary measure, according to Giguere via Jonas Siegel of Eat, Sleep, Leafs:
"You don't want to pull yourself like that," Giguere said. "But at that point I think it would've been selfish for me to stay in and not be 100%."
Over the past month of so Giguere has established himself as a leader in the lockeroom and a veteran presence amongst a young team, especially with captain Dion Phaneuf injured. His counterpart Jonas Gustavsson relieved Giguere and made six wins en route to the victory.
This injury does not sound serious but Gustavsson could get the next start regardless.
Jean-Sebastien Giguere, Toronto Maple Leafs


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We've come a long way – An interview with Shwu Jiau Teoh
Posted by Mary Schneider under ICT-KM Program, Knowledge Sharing | Tags: File Cabinet, Google Apps, google sites, Social Media, social media tools, WorldFish |
In the beginning …
Shwu Jiau Teoh
Way back in the early 1980s, there was no email in CGIAR. When scientists wanted to collaborate with each other, they did so using the technology available at that time: phone, fax, telex and cable. Some of these communication methods were often slow and unreliable, and always expensive – factors that had a direct bearing on critical research efforts. Then in 1985, with the advent of email across the CGIAR System, things began looking up. Almost overnight, day-to-day communications became much faster and cheaper. However, long-distance collaborative efforts could still be slow and, at times, confusing.
The age of social media
Fast forward to the 21st century and we have a completely different scenario on our hands. Welcome to the Age of Social Media!
To find out how today's scientists are collaborating in the CGIAR, we caught up with WorldFish GIS specialist Shwu Jiau Teoh at her office in Penang, Malaysia.
"I feel social media is changing the way some CGIAR researchers work with partners and present the results of their research," she says. "For example, my team uses Google Sites to share documents and collaborate. It's easy to create a website using the Google Sites template. You don't even have to have a programming background – I picked it up in a few minutes. It's ideal for accessing and sharing information and it's free."
Shwu Jiau is also impressed with the various features and functions of Google Sites.
"My team in Penang needs to be able to share information and collaborate with our Chinese partners while working on our project Valuing Living Aquatic Resources of Wetlands in China, led by Dr. Suan Pheng Kam," she explains. "We started using the site last May, when the project first got underway, and we feel that the 10GB of storage (Google Apps standard edition) is more than enough for our needs. We have created content on a public Google site, so that visitors are informed of our work as the project advances. But we also set up a restricted site available to just our team members for sharing knowledge and documents in one place. The site settings allow us to easily assign different levels of permission to our members.
"Any changes to a document are tracked in a history archive, so we can follow the evolution of a document as it is accessed and changed by the various team members. There's also a calendar, a section where members can see announcements in real time, and a page for project and research documentation. A dashboard page, which is by default a two-column webpage with four placeholder gadgets, automatically gives an overview of the project: an embedded calendar with the most recent posts from the announcements page, a list of updated files from the project document page and links to the different research components page.
Of all the easy-to-use features available on Google Sites, this GIS specialist feels the File Cabinet, in which project documents and literature are stored, to be the one that team members value the most.
"Without Google Sites, we would have to communicate via email, which wouldn't be convenient when we want to share large files, as some mail boxes have limitations," she explains. "The File Cabinet is very useful for storing our reports and research literature. In addition, it immediately displays the latest version of all our documents. This makes it easy for team members to keep up to date and also helps with the compilation of donor reports – this is easily done by referring to the related documents available on the project page, without having to search through all the annexes.
"We also use the File Cabinet when we want to prepare material for a workshop and need input from our Chinese counterparts. At the conclusion of a workshop, we usually upload material from the event onto the site for the team members to access and also embed into the site a Picasa slideshow that displays the workshop photo album. There's no way we could go back to using just email to accomplish this."
It looks as if Shwu Jiau and her team have their feet firmly planted in the 21st century.
For those who are already using Google Sites, we'd love to hear your story, too.


Boston Police Commissioner Ed Davis told members of the Justice Department's terrorism task force on Wednesday that local law enforcement needs to receive information on terrorist threats more quickly.
Just hours before the Boston Marathon bombing suspect was set to be arraigned in federal court, Boston Police Commissioner Ed Davis told members of the Justice Department's terrorism task force on Wednesday that local law enforcement needs to receive information on terrorist threats more quickly.
Davis testified on Capitol Hill as part of the Senate Homeland Security and Governmental Affairs Committee's review of the Boston Marathon bombing.
"There is a gap with information sharing at a higher level while there are still opportunities to intervene in the planning of these terrorist events," Davis said, urging lawmakers to write into the task force's mission a requirement that information be shared, according to the Associated Press.
The Associated Press reported that the chairman of the Homeland Security Committee complained that the FBI, the lead investigative agency in the bombing, had refused to attend the meeting. Questions linger about what the FBI knew in advance that might have helped prevent the April 15 bombings that killed three people and wounded more than 260.
"It is this committee's responsibility find out how we did not see it coming," said Rep. Michael McCaul, R-Texas. "We are going to find out what happened, what went wrong, and how to fix it."
According to the Associated Press, experts testifying in the Senate said Boston's response actually was exemplary, in part because the city had years ago built relationships and disaster plans among law enforcement, medical personnel and other responders.
Davis said that in the aftermath of the bombing, the FBI improved information sharing but that more needs to be done, AP reported. He also said cellphone service was overloaded immediately after the attack.
Phones "were rendered completely useless as a means of communication at the scene," he said, adding that "satellite phone technology is not effective for indoor command posts and communication across multiple bodies."
According to the Associated Press, Davis said law enforcement needs "a secure bandwidth in a public safety spectrum dedicated exclusively to public safety use now, as it is the only way to communicate during an event of this magnitude."


Have you started thinking about 2012? The world is supposedly going to end next year, so this is the year to give your goals all you've got. I'm diligently working on my New Year resolutions, goals and projects and am looking forward to sharing them later this weekend.
I find it easiest to think about what I've liked and am happy about in the last year and where I feel I have room to grow. I've accomplished a ton in 2011 - including running my first 10K, 5 miler, 11.5-mile race and half marathon. I also took on an awesome project by becoming the Director of Ambassador Outreach for the Fort2Base Race. I'm ready to use these as a great springboard for an even better 2012.
Don't wait until you're two champagnes into New Year's Eve. Get out a piece of paper and get started with planning SMART goals.


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Japan boosts internationalisation funding in a bid to climb global rankings
The Japanese government announced additional funding last month for 37 leading universities. The new spending aims to boost the international competitiveness of Japanese higher education overall, with the goals of further driving the country's ambitious internationalisation efforts in higher education and placing more Japanese institutions among the top-ranked universities in the world.
Education Minister Hakubun Shimomura announced the details of the "Top Global University Project" at a 26 September press conference. Designed as a 10-year project, the initiative places the participating institutions in two categories of "superglobal universities": "Type A" are the country's top research and education institutions while "Type B" are expected to pioneer internationalisation efforts in Japanese higher education. The institutions were selected from 104 applicants by a ministry-appointed panel.
The 37 superglobals
"Type A" institutions will each receive about ¥420 million (US$3.8 million) in annual subsidies, whereas "Type B" universities will receive roughly ¥170 million (US$1.6 million) annually over the term of the project.
The 13 "Type A" institutions are as follows:
National universities: Hiroshima University, Hokkaido University, Kyoto University, Kyushu University, Nagoya University, Osaka University, Tokyo Institute of Technology, Tokyo Medical and Dental University, Tohoku University, University of Tokyo, University of Tsukuba.
Private universities: Keio University, Waseda University.
The researcher exchange facility at Hiroshima University
The 24 "Type B" universities are:
National universities: Chiba University, Kanazawa University, Kumamoto University, Kyoto Institute of Technology, Nagaoka University of Technology, Nara Institute of Science and Technology, Okayama University, Tokyo University of the Arts, Tokyo University of Foreign Studies, Toyohashi University of Technology.
Public universities: Akita International University, University of Aizu.
Private universities: Hosei University, International Christian University, International University of Japan, Kwansei Gakuin University, Meiji University, Rikkyo University, Ritsumeikan Asia Pacific University, Ritsumeikan University, Shibaura Institute of Technology, Soka University, Sophia University, Toyo University.
Each institution will now be expected to carry out its individual plan for internationalisation and development, and, as such, each is immediately now a university with renewed potential as an international partner. The Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) clearly expects that project-related initiatives will span a broad range of international engagement, including student exchange, faculty exchange, joint research, transnational education programmes, and perhaps even the establishment of schools or campuses operated in collaboration with foreign partners.
Some of the participating institutions have publicly announced aspects of their plans under the "Top Global University Project". Hiroshima University, for example, aims to be a top-100 ranked institution, and plans to boost the number of students it sends abroad by more than 400%, from 423 this year to 1,835 in 2023. It similarly intends to double the number of international students it hosts (up to 3,600 by 2023), and increase the number of classes taught in foreign languages from 397 today to 3,357 by 2023.
Meanwhile, Okayama University, a Type B institution, plans to significantly expand courses offered in English, and to achieve a dramatic increase in the number of students scoring 700 or higher on the TOEIC English exam. Ritsumeikan University, also in Group B, plans to build new curriculum and courses focused on the development of problem-solving skills in collaboration with Japanese companies with substantial international operations.
"If the 37 universities meet their goals, half of their teaching staff would come from abroad and a fifth of their classes would be taught in English," said Minister Shimomura.
In danger of being left behind
The Nikkei Asian Review provides the rationale for the Japanese government's continuing focus on internationalising the country's leading universities.
"Over 20% of teaching staffs at many top universities abroad are foreigners. For example, 30% of Harvard's and 40% of Cambridge's are international. In Japan, the figure averaged just 4% in fiscal 2012.
International students enrolled in bachelor's and master's programmes also only account for 2.9% of all students in Japan, which falls significantly under the 7.8% average among member states of the Organization for Economic Cooperation and Development (OECD).
The country is falling behind on international cooperation in research as well. Just 26.4% of Japanese publications are co-authored by foreign researchers, compared with 52.4% of British publications and 33.4% of US ones."
As in many other countries around the world, the global university ranking tables provide a handy scorecard for Japanese educators and policy makers. And Japan clearly has ambitions to place more institutions in the world's ranking tables – and at as high a position as possible.
Currently, only the University of Tokyo (#23) and Kyoto University (#59) appear in the Top 100 of the Times Higher Education World University Rankings for 2014/2015. Both institutions are also found in the 2014 Shanghai ranking, and are joined there by another Group A institution, Osaka University (#78).
The field opens up a bit more in the QS World University Rankings for 2014/15 where we again find The University of Tokyo (#31), Kyoto University (#36), and Osaka University (#55), but this time they are joined in the Top 100 by two other Group A universities: Tokyo Institute of Technology (#68) and Tohoku University (#71).
Building on ambition
The "Top Global University Project" builds on a foundation of ambitious internationalisation plans already set out by the Japanese government. These include the Global 30 Project, through which leading Japanese universities offer complete degree programmes in English, and a broader goal to build international enrolment in the country to 300,000 students by 2020.
The government has also taken steps to expand support for outbound student mobility and has announced reforms to further internationalise Japanese curricula through the expansion of English language instruction in primary grades as well as a proposed redesign of university entrance exams.
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This entry was posted in Asia, Higher Education, Japan, Regions and tagged tertiary education overseas, colleges with international students, student mobility, staff mobility, transnational education.
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Trang chủ / Tin tức & Sự kiện / bolivia climate map
bolivia climate map
Annual rainfall ranges from about 40 inches (1,000 mm) in the south to 70 inches (1,800 mm) or more in the far north, with a pronounced summer maximum. In Tiquipaya, the average annual temperature is 17.0 °C | 62.6 °F. Near Mount Sajama, at an elevation of 14,000 feet (4,300 metres), one of the highest forests of khena trees has survived. However, present-day Aymara associate themselves with the ancient civilization of the Tiwanaku culture which had its capital at Tiwanaku, in Western Bolivia. The Bolivia Weather Map below shows the weather forecast for the next 12 days. Hi/Low, RealFeel®, precip, radar, & everything you need to be ready for the day, commute, and weekend! Bolivia is among the nations least responsible for climate change—in 2010, it released about 13 million metric tons of carbon dioxide emissions … It is characterized by a humid and warm climate in the eastern part of the country. Among the thousands of different trees are Hevea brasiliensis (the most common rubber tree), Bertholletia excelsa (the source of Brazil nuts), and mahogany. In Cochabamba, the average annual temperature is 17.0 °C | 62.7 °F. La Paz Although Bolivia lies wholly within the tropics, it possesses every gradation of temperature from that of the equatorial lowlands to arctic cold. Peru Weather, climate and geography ... Brazil and Bolivia to the east, and Chile to the south. Control the animation using the slide bar found beneath the weather map. Bolivia - Bolivia - Languages and religion: Spanish and 36 indigenous languages are official in Bolivia per the 2009 constitution. However, the highlands experience minimal rain causing the days to become cloudy. By signing up for this email, you are agreeing to news, offers, and information from Encyclopaedia Britannica. Based on weather reports collected during 1985–2015. Many Indians, particularly in the cities, market towns, and new colonies, speak or understand Spanish. Bolivia is a landlocked country in South America, bordering Brazil, Paraguay, Argentina, Chile and Peru. © Climate-Data.org / AM OP / OpenStreetMap contributors. The climate here is considered to be a local steppe climate. Tola (a tough, wind-resistant shrub) and mosslike cushions of yareta, both widely used for fuel, are well distributed, along with cactus scrub. The wettest period is November to March, which, in extreme circumstances, may induce landslides in mountainous areas, and cause certain roads to become impassable. The Köppen-Geiger climate classification is BSk. Potosí – Potosí. There are basically five separate climatic regions: The Andes and Altiplano, the Yungas and Chapare, the temperate valleys, the Chaco and […] Did the Incan people rule over ancient Mexico? Date: 20 February 2016: Source: Derived from World Köppen Classification (with authors).svg. The rainfall here is around 394 mm | 15.5 inch per year. Did Chile gain its independence in 1818? In the Valles region to the south there is a general covering of drought-resistant grasses, shrubs, and small trees, and in the southern foothill zone there is a strip of deciduous forest with such trees as the walnut and quebracho, the latter being a source of tannin and timber. Climate Change Knowledge Portal. The climate here is considered to be a local steppe climate. English: Bolivia map of Köppen climate classification. The temperature here averages 8.0 °C | 46.4 °F. Beni In Cochabamba, there is little rainfall throughout the year. In Bolivia, the climate is very varied, temperatures depend on the area where you are, in the Highlands comprised of La Paz, Oruro and Potosí climate is cold, in the Llanos comprised of Cochabamba, Sucre and Tarija climate is warm and tropical comprised of Santa Cruz, Beni and Pando climate … Characteristic trees include the green pine, aliso (a shrublike tree), laurel, cedar, tarco (a shade tree producing masses of yellow-white flowers), and saúco (which yields fruit used to make medicinal syrups). Regional Characteristics Differences in topography and climate separate Bolivia's lowlands and highlands into distinct areas. Climate data and weather averages in Tarija. The climate of the area north-east of the Andes, in the regions of Beni and Pando, is hot and humid with heavy rains especially between October and May, Trinidad for example, has an annual average of 1,800 mm of rainfall, the maximum daytime temperature averages are around 30°C throughout the year. Uyuni Totora reeds, which grow on the shores of Lake Titicaca, are used for thatching, feeding livestock, and making the Indian boats called balsas. The Climate Change Knowledge Portal is a hub of information, data and reports about climate change around the world. The climate here is classified as BSk by the Köppen-Geiger system. San José de Chiquitos Bolivia has three well-defined geographic zones — high plateau (altiplano), temperate and semitropical valleys of the eastern mountain slopes (yungas), and tropical lowlands (llanos) of … Precipitation, which ranges up to 53 inches (1,350 mm) annually, occurs throughout the year but is heaviest between December and February. The eastern lowlands include all of Bolivia north and east of the Andes. Pando During the year there is little rainfall. They are laden with sand, high humidity, and dust and last for a few days. Bolivia - Bolivia - Climate: Although Bolivia lies wholly within the tropics, it possesses every gradation of temperature from that of the equatorial lowlands to arctic cold. The climate in Oruro is referred to as a local steppe climate. Tiquipaya Travel weather and climate description. Bolivia's geography is defined by extremes, with a climate that varies with altitude from humid and tropical to cold and semiarid. Bolivia lies in the Southern Hemisphere, albeit at tropical latitudes, so its seasons are reversed in comparison with the Northern Hemisphere. This climate is considered to be BSk according to the Köppen-Geiger climate classification. Bolivia's landscape. The Geography of Bolivia Total Size: 1,098,580 square km Size Comparison: slightly less than three times the size of Montana Geographical Coordinates: 17 00 S, 65 00 W World Region or Continent: South America General Terrain: rugged Andes Mountains with a highland plateau (Altiplano), hills, lowland plains of the Amazon Basin Geographical Low Point: Rio Paraguay 90 m Covering an area of 1,098,581 sq.km (424,164 sq mi), Bolivia is the 5th largest country in South America and the largest landlocked nation in the Southern Hemisphere. Map of Bolivia . The area becomes muddy with the floods resulting in increased humidity, heat and mosquitoes. Potosí, Bolivia / Jenny Mealing, Flickr / CC BY 2.0. Western Bolivia is generally colder due its high altitude - similar to the Andes highlands. Select from the other forecast maps (on the right) to view the temperature, cloud cover, wind and precipitation for this country on a large scale with animation. La Paz has dry periods in May, June, July and August. True Amazonian rainforest (selva amazónica) occurs only in the far north in the department of Pando and adjacent areas. Bolivia - Climate data - Climate information and average monthly temperature, humidity, rainfall, snowfall, daylight, sunshine, UV index, and sea temperature for all cities and towns. The rainfall here is around 393 mm | 15.5 inch per year. Occasional cold winds called surazos blow from the south, lowering temperatures abruptly. Part of Beni suffers from extensive flooding beginning in March or April, toward the end of the summer rainy season. Average temperatures range between 45 and 52 °F (7 and 11 °C) during the day, occasionally reaching as high as 60 °F (16 °C), but temperatures at night are much colder and fall below freezing during the winter. The best time and weather to travel to Bolivia 2020. Farther north in the Oriente, grass, palm, and swamp savannas extend into Beni. Santa Cruz The highest point in Bolivia is the Nevado Sajama with 6542 m/ 21463 ft. The Pacific Ocean lies to the west. The temperature here averages 9.1 °C | 48.3 °F. The prevailing climate in Cochabamba is known as a local steppe climate. Although it is a country of climatic extremes, it can be visited all year round. CLIMATES In general the climates in Bolivia are dictated mostly by altitude not latitude. In the 17th century was one of the largest and richest cities in the world, numerous very ornate and interesting structures were built … The best time to visit Bolivia depends on where you go. Although comprising over two-thirds of the national territory, the region is sparsely populated and, until recently, has played a minor role in the economy. Potosí In Tarija, there is little rainfall throughout the year. Eucalyptus and pine trees have been introduced around the shores of Lake Titicaca and in sheltered valleys. La Paz When th… The prevailing climate in Cochabamba is known as a local steppe climate. Here you can query, map, compare, chart and summarize key climate-related information. Cattle raising and logging operations, many of them illegal, place an increasing strain on forested areas throughout the Yungas and the Oriente. Precipitation here is about 556 mm | 21.9 inch per year. Bolivia is a landlocked nation located in west-central South America. Thus the climate is also very diverse. El Alto Oruro Bolivia from The World Bank: Data. Rurrenabaque. The climate in the eastern tropical low lands of Bolivia is hot and humid, the area of the country spans more than half of Bolivian territory. The capital city of Tiwanaku dates from as early as 1500 BC when it was a small, agriculturally based village. The climate of Bolivia varies drastically from one ecoregion to the other, from the tropics in the eastern llanos to polar climates in the western Andes. Bolivia has several geographical areas and climates, and the three predominant ones are Andean (28% of the territory), sub-Andean (13%), and plains (59%). The cinchona, or quina tree, from which quinine is made, and the coca shrub, the source of cocaine, are also indigenous there. Large stretches of the Altiplano are planted in field crops. This location is classified as BSk by Köppen and Geiger. Oruro This location is classified as BSk by Köppen and Geiger. The region now known as Bolivia had been occupied for over 2,500 years when the Aymara arrived. In plains and lowlands, the climate is equatorial in the far north and tropical in the center-south, while in the plateau and the Andean mountain peaks, it's colder and generally quite arid. In Cochabamba, there is little rainfall throughout the year. Previously only Spanish, Aymara, and Quechua were official languages of the country. Author: Enhanced, modified, and vectorized by Ali Zifan. Here are some average weather facts we collected from our historical climate data: The months of January, February and December have a high chance of precipitation. The rainfall here is around 518 mm | 20.4 inch per year. During the year, there is little rainfall in Tiquipaya. Bolivia Weather, climate and geography Weather and climate Best time to visit. A historical mining city next to the largest silver mine in the world. The climate of Bolivia Bolivia is a country centrally situated in South-America. Annual Weather Averages in Tarija. The southern areas have a mild climate whereas the western part is dry. Bolivia has a temperate climate but temperatures can differ dramatically between day and night. Huge expanses of the southern Altiplano are saline and barren, but ichu (a coarse bunchgrass) is common in the north, where it is grazed by llamas. Tarija In the north, however, Lake Titicaca has an important moderating influence, and in bright sunshine winter temperatures may reach as high as 70 °F (21 °C). The mean annual temperatures vary between 60 and 68 °F (16 and 20 °C). The lowlands experience heavy rains that cause floods. In the winter the Andean skies are often a deep blue. There are three natural zones: The Costa region, which contains Lima (the capital), is a narrow coastal plain consisting of large tracts of desert broken by fertile valleys. Get the forecast for today, tonight & tomorrow's weather for La Paz, La Paz, Bolivia. The rainy season is from December to March, but precipitation varies greatly throughout the highlands. The climate in Potosí is referred to as a local steppe climate. The Yungas region of the Andean foothills is clad in luxuriant mountain rainforest that includes an enormous variety of tropical hardwoods, dyewoods, medicinal and aromatic plants, and fruit trees. At lower elevations in the Oriente, vegetation is strongly controlled by the degree of waterlogging that occurs and by the length of the dry season. Santa Cruz de la Sierra Formation of Liberal and Conservative parties, The rise of new political groups and the Bolivian National Revolution. Whole year UV Index table for Bolivia ☀ Water Temperature in localities in Bolivia Weather phenomena recorded in previous years in Bolivia ☃ Long-term 30 day weather forecast for Bolivia ☔ Bolivia month-by-month travel guide to weather conditions and best times to visit. Villazón There, strips of tropical forest line the riverbanks, whereas more continuous forest appears in eastern and northeastern Bolivia. During the year there is little rainfall. Other versions: Köppen climate classification; Köppen climate … The west half of the country is dominated by the huge Andes mountains, with steep slopes and snow-capped peaks. Be on the lookout for your Britannica newsletter to get trusted stories delivered right to your inbox. The average annual temperature is 17.7 °C | 63.9 °F in Tarija. Further south in the region of Santa Cruz de la Sierra, the climat… The rainfall here is around 590 mm | 23.2 inch per year. Sucre In the south the Chaco is scrub-covered, with scattered stands of quebracho giving way northward to a region of semideciduous tropical forest. Chuquisaca In the Andes, contrasts in temperature and rainfall depend more on elevation and cloud cover than on distance from the Equator, and cold winds sweep the Altiplano year-round. As observed on the physical map of Bolivia … Bolivia's rainy season starts from November to March. Sort fact from fiction in this journey through Latin America. Cloudless skies and remarkably clear air bring distant Andean peaks sharply into focus, providing beautiful vistas across the Altiplano. Bolivia Weather Conditions Bermejo 71° Calacoto 49° Camiri 71° Cobija 70° Cochabamba 66° Colcapirhua 66° Concepcion 75° Cotoca 77° La Paz 46° Montero 78° … Differences in topography and climate separate the lowlands into three areas. The summers are warm, humid in the east and dry in the west, with rains that often modify temperatures, humidity, winds, atmospheric pressureand evaporation, giving place to very different climates. Today's and tonight's La Paz, La Paz, Bolivia weather forecast, weather conditions and Doppler radar from The Weather Channel and Weather.com Native quishuara and khena trees can still be found on the Altiplano. Potosí On the low plains of the Oriente the climate is hot, averaging 73 to 77 °F (23 to 25 °C) or higher in the south and up to 80 °F (27 °C) in the north. The best time of year to visit La Paz in Bolivia. The Valles have brighter conditions and less precipitation than the Yungas, as well as somewhat warmer temperatures. In the Andes, contrasts in temperature and rainfall depend more on elevation and cloud cover than on distance from the Equator, and cold winds sweep the Altiplano year-round. The best time to visit the highland areas is April to October — this is the cooler winter period, but it should be dry with plenty of sunshine, with temperatures around the mid-teens. The description under each title shows the dates of coverage of the publication, the parameters you can find in the data, and a list of the volumes that the Library holds … Weather in Bolivia. Read more about Bolivia geography on our Bolivia maps page. According to Köppen and Geiger, this climate is classified as BSk. The mean elevation is 1192 m/ 3910 ft. Bolivia Climatological Data The publications shown below contain climatological data for Bolivia. Cochabamba Together with the neighbouring country of Paraguay Bolivia is the only country on the continent without a coastal line. In stark contrast, clouds of moist air from the Oriente fill the valleys of the Yungas throughout the year, leaving the humid atmosphere rich with the smell of vegetation. Exploring Latin America: Fact or Fiction? The basic weather pattern of Bolivia is the wet and the dry season, which happens at the same time country-wide. The rains make the countryside look green and facilitate the growt… Located at 4,090 m height. Tarija, Cochabamba [189510] Climate is the statistics of weather, usually over a 30-year interval.
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Ford have launched a nationwide awareness campaign in collaboration with Time to Change; a well-established mental health movement that is run by two charities', Mind and Rethink Mental Illness. Their aim is to reduce the stigma surrounding mental health and encourage people to "break the silence on mental health".
Ford's national awareness film promotes the front seat of a vehicle as a 'safe place to talk'. Their research shows over 67 percent (two thirds) of people, were more comfortable discussing their issues whilst in a vehicle.
Here are the five 'Top Tips' from Time to Change and Ford.
Statistics show that around one in four people in the UK experience some kind of mental health problem, with half saying that isolation is worse than the condition itself.
Young men have been identified as particularly vulnerable with only a third of them (34 percent) saying they would openly discuss their feelings, and only 31 percent saying it would be embarrassing to seek help.
With both Andy Barratt and Charles Bilyeu (Chief Executive Officer, Ford Credit Europe Bank) signing the Time to Change Employer Pledge, this makes Ford one of the first automotive manufacturers to promote metal health awareness within the workplace. Working in conjunction with Mental Health First Aid England, the pledge is there to commit the organisation to providing more mental health support for all of their employees.


text:
Dr. Martin Luther King and Ms. Ella Baker
Ted GlickJanuary 15,2018
Ella Baker. Photo-ebay
I woke up this morning thinking about Dr. Martin Luther King, Jr. And then I began thinking about Ella Baker, whose model of "group-centered leadership" I have recently been meditating on and writing about.
I heard Dr. King speak in person twice during the 60's, once at Grinnell College in Iowa a few months before he was assassinated. When he was killed 50 years ago, on April 4th, 1968, I began what became 50 years of progressive activism by posting a petition to Congress on the wall of the student mail room that night. About half the students had signed it by the end of the following day and I sent it off to Mike McCormack and Hubert Humphrey, the leaders of the House and Senate.
I met and worked with Ella Baker for several years in the mid- to late-70's after I moved to New York City. Ms. Baker was part of the leadership for a while of a group I was working with, the Mass Party Organizing Committee, and I have always been so grateful for that privilege. I would like to think that I learned much about how to be an effective organizer from the time spent with her and the things about her that I learned from others.
Dr. King and Ella Baker were the two primary leaders of the Southern Christian Leadership Conference in its first couple of years of existence, between 1958 and 1960. King was the heart, soul and speaking voice of SCLC; Baker was the Executive Secretary. Baker was a public speaker too, but she was primarily the behind-the-scenes, get-things-done, relationship-building person. Her vision in doing so was for the emergence of a grassroots-based, mass movement and organization that could take on structural racism and fundamentally transform US society, with Black people at the center of the leadership of that movement.
Ella Baker had been active in the Black civil rights and freedom movement since the late 1920's. She had traveled throughout the deep South in the 1940's for the NAACP, actively supporting local organizers and encouraging the building of strong grassroots organizations which operated in a cooperative way. She was a strong advocate for a shared leadership model and an emphasis on empowering the members of the organization and the new people getting involved: a continually-empowering mass movement.
Ms. Baker had problems with the decision-making structure of SCLC, as explained clearly in Joanne Grant's biography, "Ella Baker, Freedom Bound." The decision-making structure, as explained there, was essentially Dr. King making the major decisions, with input from others.
With the emergence of the student desegregation sit-ins at lunch counters in the deep South in 1960 and the creation of the Student Nonviolent Coordinating Committee, Ms. Baker shifted her time and energies to SNCC. She was a major supporter of the organization and took part in the decision-making process, but usually in the capacity of an active listener who occasionally asked questions.
Grant explains it this way: "Often, her questions directed the discussions. Her technique was much like that of Nelson Mandela, who had learned it from his mentor, a [traditional] chieftain. Mandela wrote in his autobiography, Long Walk to Freedom, 'I have always endeavored to listen to what each and every person in a discussion had to say before venturing my opinion. Oftentimes, my own opinion will simply represent a consensus of what I heard in the discussion.'" (p 136)
Our individual movements and our movement of movements can accomplish much in 2018. The political winds are clearly blowing in a progressive direction, evidenced most recently by the defeat of Trump-backed bigot Roy Moore in the US Senate race in Alabama. The Republicans, and corporate Democrats, are in trouble. But unless we consciously utilize, as much as possible, the group-centered, cooperative, grassroots-empowering, consensus-seeking methods of decision-making, consciously interact with one another and new people in a supportive and strengthening way, whatever gains we make will be much less than what they could be, much less than what our peoples and our damaged world so badly need.
Ted Glick has been a progressive activist and organizer since 1968. Past writings and other information can be found at http://tedglick.com, and he can be followed on Twitter at http://twitter.com/jtglick.
Dr. King and Ms. Baker
Ted Glick
Ella Baker
Mike McCormack
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Nuclear Option Clears Path for African American Judge Robert L. Wilkins, And Two Women


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We know that selling your home can feel like a daunting task. Our Listing Team is specially trained to ensure the process is as seamless as possible. Selling your home will begin with a thorough pre-sale consultation, in which will discuss the overall strategy specific to your home.
Our Client concierge will be pleased to coordinate the execution of any and all tasks to help prepare your property. From contractors to cleaners, we handle coordination - so you don't have to.


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text:
using System.Web.Mvc;

namespace VsixMvcAppResult.UI.Web.Areas.UserAccount
{
    public static class UserAccountUrlHelper
    {
        public static string Account_LogOn(this UrlHelper helper)
        {
            return helper.Action("LogOn", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_LogOff(this UrlHelper helper)
        {
            return helper.Action("LogOff", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_Register(this UrlHelper helper)
        {
            return helper.Action("Register", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_CantAccessYourAccount(this UrlHelper helper)
        {
            return helper.Action("CantAccessYourAccount", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_ChangePassword(this UrlHelper helper)
        {
            return helper.Action("ChangePassword", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_ResetPassword(this UrlHelper helper)
        {
            return helper.Action("ResetPassword", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_Activate(this UrlHelper helper)
        {
            return helper.Action("Activate", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_Dashboard(this UrlHelper helper)
        {
            return helper.Action("Dashboard", "UserAccount", new { Area = UserAccountAreaRegistration.UserAccountAdminAreaName });
        }
        public static string Account_UserUpdateLastActivity(this UrlHelper helper)
        {
            return "/UserUpdateLastActivity/UserUpdateLastActivity";
        }
    }
}

redpajama_set_name: RedPajamaGithub


To identify genetic variants underlying changes in phenotypes within and between species, researchers often utilize transgenic animals to compare the function of alleles in different genetic backgrounds. In Drosophila, targeted integration mediated by the ΦC31 integrase allows activity of alternative alleles to be compared at the same genomic location. By using the same insertion site for each transgene, position effects are generally assumed to be controlled for because both alleles are surrounded by the same genomic context. Here, we test this assumption by comparing the activity of tan alleles from two Drosophila species, D. americana and D. novamexicana, at five different genomic locations in D. melanogaster. We found that the relative effects of these alleles varied among insertion sites, with no difference in activity observed between them at two sites. One of these sites simply silenced both transgenes, but the other allowed expression of both alleles that was sufficient to rescue a mutant phenotype yet failed to reveal the functional differences between the two alleles. These results suggest that more than one insertion site should be used when comparing the activity of transgenes because failing to do so could cause functional differences between alleles to go undetected.
Understanding the genetic basis of phenotypic change remains a pressing challenge for evolutionary biology. Addressing this challenge requires the identification of the genes contributing to phenotypic divergence as well as the specific changes within those genes that alter their function (Stern and Orgogozo 2008). Linkage mapping and genome-wide association studies (GWAS) are often used to identify regions of the genome associated with phenotypic divergence (Martin and Orgogozo 2013); however, these approaches must be supplemented with functional tests to demonstrate the phenotypic consequences of individual genes and sequence changes. This functional testing is often accomplished through transgenic analysis that evaluates the effects of a specific gene or region of a gene in different genetic backgrounds.
In Drosophila, the activity of divergent alleles is typically compared using transgenes inserted into the genome by transposon-mediated transformation (Wittkopp 2006). Most transposons used for this purpose (e.g., P-elements, piggyBac, and Hermes) insert a transgene semirandomly into the genome (Engels 1996; Garza et al.1991; Guimond et al. 2003; Handler and Harrell 1999; Smith et al. 1993; Spradling and Rubin 1983), which is not ideal because the genomic position of a gene can affect its activity, a phenomenon known as "position effect" (Sturtevant 1925; Wilson et al. 1990). These position effects can result from chromatin structure at the insertion site (Huisinga et al. 2016; Levis et al. 1985; Wilson et al. 1990) and/or interactions between the sequence of the transgene and the surrounding DNA that affect expression of the transgene (Venken and Bellen 2007; Wilson et al. 1990). The former generally affects the expression level of the transgene, whereas the latter can impact its expression level and/or spatiotemporal regulation. The extent of position effects has been hypothesized to be the product of two variables: (i) the strength of regulatory elements at the genomic location in which the transgene is inserted, and (ii) the susceptibility of the regulatory sequences in the transgene to altered activity (Wilson et al. 1990). The addition of insulator sequences flanking a transgene can reduce the effects of surrounding genomic context on its activity (Gdula et al. 1996; Kuhn and Geyer 2003; Silicheva et al. 2010).
Position effects are especially problematic when comparing activity among transgenes expected to vary in subtle ways. Targeted insertion of transgenes in Drosophila, most notably using the bacteriophage ΦC31 integrase system (Groth et al. 2004), can help control for position effects by inserting each transgene of interest into the same genomic position of otherwise identical genomes (Venken and Bellen 2005). With large collections of "landing sites" (sequences that mediate integration of the transgene) for ΦC31-mediated transformation available (Bateman et al. 2006; Bischof et al. 2007; Venken et al. 2006), this method has become the standard for comparing the activity of related alleles in Drosophila. Typically, such a study compares a set of transgenic lines in which each transgene is integrated independently into a chosen landing site, with a single landing site used in most cases (e.g., Cande et al. 2009; Duncan et al. 2010; Frankel et al. 2010; Haley et al. 2010; Joshi et al. 2010; Kalay and Wittkopp 2010; Perry et al. 2011; Rebeiz et al. 2011; Sayal et al. 2011). The use of a single landing site for such studies is justified by the assumption that all alleles compared will be affected similarly by the surrounding genomic context (Wimmer 2005). But is this true? Are sets of related transgenes influenced similarly by the surrounding DNA sequence?
Here, we test this assumption by examining the impact of position effects on a comparison of orthologous alleles that contribute to phenotypic divergence between a pair of closely related Drosophila species. Specifically, we compare the effects of tan alleles from D. americana and D. novamexicana integrated into the D. melanogaster genome at five different genomic locations. D. americana and D. novamexicana diverged ∼400,000 years ago (Caletka and McAllister 2004; Morales-Hojas et al. 2008) and have evolved dramatic differences in adult pigmentation (Throckmorton 1982); D. americana has a brown body, whereas D. novamexicana has a yellow body (Figure 1). Prior work has shown that these differences in pigmentation are due in part to divergent sites located in the tan gene (Wittkopp et al. 2009). As described below, we found that position effects influenced whether or not a difference in activity could be detected between these two species-specific alleles of tan. Further analysis showed that the ability to detect a difference in activity was related to level of expression from the tan transgene at each site. These findings suggest that differences between transgenes should be assessed using multiple landing sites.
Body color of D. americana and D. novamexicana. D. novamexicana (right) has evolved lighter body pigmentation since it diverged from the common ancestor shared with D. americana (left). D. americana has retained the darker body pigmentation shared by all other members of the virilis group.
Previously constructed transgenes containing D. americana or D. novamexicana tan (Wittkopp et al. 2009) were injected into D. melanogaster using the ΦC31 integrase system. The transgenes contained all exonic and intronic sequences of tan, as well as 4.1 kb of sequence 5′ of tan and 3.6 kb of sequence 3′ of tan in a piggyBac vector (Horn and Wimmer 2000) containing an attB site used for ΦC31-mediated transformation and Pax6-EGFP, an eye-expressing green fluorescence marker used to detect successful integration (Wittkopp et al. 2009). In addition to noncoding and synonymous changes, these D. americana and D. novamexicana tan transgenes differ by two amino acids; however, these amino acid differences are not fixed between species and are thus unlikely to be responsible for the species-specific differences in pigmentation (Wittkopp et al. 2009). Each D. melanogaster host genotype carried a transgene on the X-chromosome using the vasa cis-regulatory sequences to express the ΦC31 integrase specifically in the germ-line and a single attP site located on the second (51C – BDSC #24482, 58A – BDSC #24484), third (86Fa – BDSC #24486, 86Fb – BDSC #24749), or fourth (102D – BDSC #24488) chromosome (Bischof et al. 2007). These lines were selected because they contain an eye-expressing red fluorescent protein (RFP) as a visible marker for the landing site; this is in contrast to the majority of strains containing attP landing sites that are commonly used, which use a copy of the yellow gene (which restores dark pigmentation in yellow mutant flies) as a visible marker for the landing site. GenetiVision (Houston, Texas) performed all DNA preparations and embryo injections according to their standard protocols (http://www.genetivision.com/). Transformant flies (expressing green fluorescent protein in their eyes) were used to establish lines homozygous for each transgene in a D. melanogaster background carrying loss-of-function mutations in the X-linked genes tan, yellow, and white. The mutant D. melanogaster tan allele allowed us to test for rescue of the tan mutant pigmentation phenotype by the heterologous tan alleles contained in the transgenes; the yellow mutant allele reduced the amount of black pigment present in these flies, providing a more sensitive assay for changes in abdominal pigmentation caused by the transgenes; and the white allele allowed for easier visualization of the eye-expressing fluorescent transformation marker.
For each line to be analyzed, virgin females were mated with males on standard yeast-glucose media at 20°. Upon formation of pupae, parents were removed and the offspring were allowed to continue development. Male offspring were collected 0–1 d posteclosion and aged to 7–8 d. Flies were stored in 10% glycerol in ethanol prior to dissection.
To harvest abdominal cuticles, 7–8-day-old males were removed individually from the 10% glycerol in ethanol solution and placed on a glass slide. Using a razor blade, the abdomen was separated from the rest of the body then cut along the lateral edge parallel with the anterior-posterior axis. The dorsal half of the abdomen was soaked overnight in a solution of phosphate buffered saline (PBS; 1.4 M NaCl, 27 mM KCl, 190 mM Na2HPO4, and 18 mM KH2PO4, adjusted to pH 7.4 with 1 M HCl). After soaking overnight, a single dorsal half of abdominal cuticle was removed from the PBS and placed on a glass slide, dorsal (cuticle) side down. Using forceps, the abdominal cuticle was cleared of any remaining debris. The cleaned cuticle was then mounted dorsal side up in polyvinyl alcohol (PVA) mounting media (BioQuip) on a clean glass slide, covered with a coverslip, and the coverslip was sealed with clear nail polish. This process was repeated for all genotypes analyzed, with 17–35 (mean = 27) flies analyzed for each genotype. To minimize effects of any day-to-day differences in dissections, all genotypes were dissected during each dissection session.
Dissected abdominal cuticles were imaged in a single session using a Leica MZ6 microscope and Scion (CFW-1308C) camera operated via TWAIN driver in Adobe Photoshop. Magnification was set to 3.2 with ring light illumination at ∼75%. At the beginning of the imaging session, auto white balance (AWB) was used, resulting in a configuration of Gamma 0.605, Red Gain -1.4 db, Green Gain 5.4 db, and Blue Gain 8.9 db with Red Boost and Blue Boost active. These settings were not changed throughout the imaging session. Imaging was conducted at night to minimize changes in ambient lighting. Images were taken slide-by-slide (2 cuticles/slides, cuticles imaged individually) with samples arranged such that no more than two cuticles from the same line were imaged consecutively. A "reference" image of the same dissected cuticle was taken approximately every 10 slides to allow us to evaluate the consistency of the image collection, processing, and analysis pipeline during the multi-hour imaging session.
All images were compiled into a single document in Adobe Photoshop and the "Levels" function was used to adjust the color of all images simultaneously so that the images more closely matched the cuticle appearance visible by eye. This ensured that an identical color adjustment was applied to all photos.
Using ImageJ (Rasband 1997-2016), the area of dorsal abdominal cuticle known as abdominal tergite 4, or "tergite A4," (insert, Figure 2) was manually selected using the polygon tool, excluding any regions containing cracks, holes, or overlapping regions. Measurements of pixel intensity (area, mean, standard deviation, mode, min, max, and median) were taken for each selection. These results were compiled into a single Microsoft Excel spreadsheet where other identifying information was then added, including imaging order (ranging from 1 to 479), allele (no transgene control, D. americana, and D. novamexicana), and landing site (control, 51C, 58A, 86Fa, 86Fb, and 102D). Since ImageJ quantifies pigmentation (pixel intensity of a grayscale image) on a 0–255 scale (dark–light), we subtracted the reported pixel intensity from 255 so that darker cuticles had a higher pigmentation score. This file was then saved as a .csv file for statistical analysis in R.
Measurements of pigmentation intensity in a control sample varied slightly during image collection. Raw median pigmentation intensity in tergite A4 (insert) is plotted against imaging order for the reference cuticle (open circles). All images were taken during in a single sitting without adjustment of lighting, focus, or other imaging parameters; the small (β = −0.0075), yet significant (p-value = 0.008), downward trend in pigmentation intensity as the imaging progressed, presumably as a result of changes in ambient lighting or other uncontrolled imaging variables. An imaging order correction was therefore applied to all measurements, as described in the Materials and Methods section. Corrected median pigmentation intensity values for the same images are also plotted against imaging order (closed circles) to show the effects of this correction.
Median pigmentation intensity of tergite A4 for each sample reported by ImageJ was analyzed using R v3.2.5 (R Development Core Team 2016). Median pigmentation was chosen for analysis instead of mean pigmentation intensity to minimize the impact of outlier (excessively white or black) pixels.
To test for systematic changes in imaging conditions that might have occurred during the imaging session, a linear regression was performed comparing median pigmentation values from the reference cuticle and the image order number. A small but significant regression coefficient (β = −0.0075, p-value = 0.008) was observed, so a correction for imaging order was applied to each median by subtracting (image order number * −0.0075) from the original median value. The differences in reference cuticle values before and after applying this correction are shown in Figure 2. Note that all analyses described below were also performed on data without this correction and produced the same pattern of statistically significant results (data not shown).
Pairwise t-tests using unpooled standard deviations were then performed on the corrected pigmentation medians to identify which comparisons among tan alleles and/or landing sites were statistically significant. Statistical significance was assessed using p-values adjusted for multiple testing by the Benjamini and Hochberg method (Benjamini and Hochberg 1995) as implemented in the pairwise.t.test function in R.
To test for differences in the expression level of transgenes inserted at each genomic location in D. melanogaster, relative expression levels of the D. americana tan transgene were measured using pyrosequencing (Wittkopp 2011). Specifically, we measured the mRNA abundance of the D. americana tan allele inserted at each genomic location relative to the mRNA abundance of the D. novamexicana tan allele inserted at the 86Fa landing site. This D. novamexicana genotype was chosen as the internal reference point for measurements of D. americana tan expression because it caused an intermediate pigmentation phenotype, suggesting it might also have an intermediate level of expression. The P14–P15 pupal stage was analyzed because D. americana and D. novamexicana tan have previously been shown to be most highly expressed during this time (Cooley et al. 2012). Pupal heads and wings were removed to avoid measuring tan expression in those tissues, focusing our measurements on expression in the thorax and abdomen where pigmentation phenotypes are most apparent.
For each landing site, both genomic DNA and total RNA were extracted from three replicate samples, each containing six dissected pupae expressing D. americana tan and six dissected pupae expressing D. novamexicana tan. cDNA was reverse transcribed from extracted RNA using a polyT primer for each sample. Both genomic DNA and cDNA were analyzed by pyrosequencing as described in Wittkopp (2011). PCR primers used to amplify the sequence analyzed (which was located in exon 7) were 5′-GATGCTGAAGTCCAGCGTGTC-3′ and 5′-biotin-CAGCCGCCAGTGACATCA-3′, and the primer used for pyrosequencing had the sequence 5′-CGAGCACGATGTCCG-3′. All measurements were then normalized to the relative expression of the D. americana tan transgene inserted at landing site 86Fa to compare expression among the D. americana tan transgenes at different landing sites.
Supplemental Material, File S1 contains pigmentation measures from all individual cuticles. File S2 contains the code used to analyze the pigmentation measures. File S3 contains the raw and analyzed pyrosequencing data.
To test the assumption that position effects are negligible when comparing divergent alleles of the same gene at a single genomic location, we transformed D. americana and D. novamexicana tan alleles into five different genomic locations in D. melanogaster (51C, 58A, 86Fa, 86Fb, and 102D). Each of these transgenic lines was then crossed with D. melanogaster yellow, white, and tan mutants (see Materials and Methods for full genotype) to move the transgenes into genetic backgrounds lacking a functional copy of the D. melanogaster tan gene. Prior work has shown that the difference in body color seen between D. americana and D. novamexicana (Figure 1) is due in part to changes in tan and that these D. americana and D. novamexicana tan transgenes significantly increase abdominal pigmentation in a D. melanogaster tan mutant (Wittkopp et al. 2009). The transgenic tan allele from the more darkly pigmented D. americana was reported to increase pigmentation significantly more than the transgenic tan allele from the more lightly pigmented D. novamexicana, indicating that there is functional divergence between these species-specific alleles that affects pigmentation (Wittkopp et al. 2009).
To determine whether the insertion site of the D. americana and D. novamexicana tan transgenes affected their relative activity, we used an analysis of variance (ANOVA) to test for significant effects on pigmentation of allelic identity of the tan transgene (D. americana or D. novamexicana), genomic location of the landing site, and the interaction between the two. All three factors were found to be statistically significant predictors of pigmentation intensity (Table 1). In other words, pigmentation differences were detected between alleles and among landing sites, and the difference between alleles differed among landing sites. The significance of this interaction term is particularly interesting because it suggests that the effects of genomic context might differ between alleles, implying that the landing site used to compare the function of D. americana and D. novamexicana alleles might alter the conclusions drawn about differences (or lack thereof) between these two alleles.
One way that the genomic context can affect a transgene is to simply silence it. To determine whether such silencing was contributing to the difference in allelic differences observed among insertion sites, we used t-tests to determine whether each transgene caused a statistically significant darkening of pigmentation in each transgenic line relative to the D. melanogaster tan mutant phenotype. Such a darkening would indicate that the transgene carried was being expressed at a level sufficient to restore at least some dark pigmentation in D. melanogaster. We found that the transgenic tan alleles from both D. americana and D. novamexicana failed to significantly alter pigmentation of the D. melanogaster tan mutant when inserted into the fourth chromosome at cytological position 102D (Table 2). This evidence of transgene silencing is consistent with prior studies showing that the fourth chromosome of D. melanogaster is highly heterochromatic (Riddle and Elgin 2006; Riddle et al. 2009) and can suppress the expression of transgenes (Salzler et al. 2013; Sun et al. 2000). Landing site 102D does not always silence transgenes, however; other transgenes inserted into the 102D landing site have been shown to be expressed during larval stages (Bischof et al. 2007; Barolo and Evans, personal communication). At each of the other four landing sites tested (all located on chromosome 2 or chromosome 3), both the D. americana and D. novamexicana tan transgenes caused a significant darkening of pigmentation relative to the tan mutant phenotype (Table 2), indicating that the transgenes were expressed and producing functional Tan protein. To determine whether the silencing of transgenes at landing site 102D was sufficient to explain the significant interaction observed between transgene identity and landing site in the initial ANOVA, we excluded flies with transgenes inserted into this site and repeated this ANOVA. We found that the two main effect terms (transgene identity and landing site) and the interaction term remained statistically significant (Table 3), indicating that the relative activity of the D. americana and D. novamexicana tan transgenes differed even among sites that allowed expression of both transgenes.
To further investigate this difference in relative transgene activity among insertion sites, we used a series of t-tests to compare the pigmentation phenotype caused by the D. americana and D. novamexicana tan alleles inserted at the same landing site. We found that the D. americana tan allele increased dark pigmentation of the D. melanogaster tan mutant significantly more than the D. novamexicana tan allele when inserted at three (58A, 86Fa, and 86Fb) of the four landing sites expressing the transgenes (Figure 3 and Table 2). The difference in activity between these two alleles was masked, however, when then transgenes were inserted into the landing site at 51C (P = 0.411, Figure 3 and Table 2). Excluding flies with transgenes at this landing site (51C) as well as flies with transgenes at the landing site that silenced the transgenes (102D) from the ANOVA described above resulted in a nonsignificant interaction between transgene allele and insertion site (Table 4), indicating that the relative effects of the D. americana and D. novamexicana tan transgenes on pigmentation were comparable at the 58A, 86Fa, and 86Fb landing sites.
Genomic location can impact the relative difference in pigmentation caused by D. americana and D. novamexicana tan alleles. Box plots of pigmentation intensity summarize, for each genotype, the range of pigmentation phenotypes observed. The median (center line), first quartile (bottom of box), third quartile (top of box), and ± 1.5 × the interquartile range (whiskers) are shown for each genotype examined. Yellow boxes along the x-axis represent the D. novamexicana allele and brown boxes represent the D. americana allele. Significant increases in pigmentation from the control were detected for all genomic locations except 102D (Table 2). Three of the other four landing sites (58A, 86Fa, and 86Fb) showed significant differences in pigmentation driven by the D. americana and D. novamexicana tan alleles, whereas the fourth landing site (51C) did not show a detectable difference in pigmentation between flies carrying the two species' alleles (Table 2). Representative images from the 25th percentile (first quartile), median, and 75th percentile (third quartile) are shown below the box plot for each genotype. The most striking differences between alleles are seen in the anterior regions outside the dorsal midline stripe.
Prior work has shown that position effects often alter expression levels of transgenes (e.g., Markstein et al. 2008; Namciu et al. 1998; Wilson et al. 1990), thus we hypothesized that the different pigmentation phenotypes resulting from different insertion sites of the transgenes might be caused by differences in transgene expression among landing sites. To test this hypothesis, we used pyrosequencing to measure the relative expression of the D. americana tan transgene among landing sites (Figure 4). Genomic locations (58A, 86Fa, and 86Fb) that showed statistically significant differences in pigmentation caused by the D. americana and D. novamexicana tan alleles had the highest levels of D. americana tan expression. The genomic location (51C) in which the D. americana and D. novamexicana tan alleles showed a significant increase in pigmentation relative to the D. melanogaster tan mutant, but no differences in pigmentation between flies carrying the D. americana and D. novamexicana tan alleles, had a lower level of D. americana tan expression. The genomic location (102D), in which neither the D. americana nor the D. novamexicana tan transgene increased pigmentation significantly relative to the D. melanogaster tan mutant, showed the lowest expression of D. americana tan among all five lines. These results confirm that different landing sites resulted in different levels of transgene expression and suggest that a threshold in transgene expression level must be reached before the different activities of the D. americana and D. novamexicana tan transgenes can be detected. We expect that this will be generally true when comparing activities of divergent alleles inserted into the same genomic location, but that the value of this threshold will likely differ depending on the strength of regulatory sequences in the transgenes, genomic context, and/or the relative difference in activity between alleles.
Genomic location impacts relative expression of the D. americana tan transgene in D. melanogaster. Expression of the D. americana tan transgene inserted at each of the five landing sites tested is shown relative to its expression when inserted in the 86Fa landing site. Circles indicate mean expression among replicate samples and the error bars show the 95% C.I. of the estimates. Note that the relative expression level of D. americana tan among landing sites correlates with the ability to detect differences in abdominal pigmentation (Figure 3). The D. americana tan transgene inserted at 58A, 86Fa, and 86Fb all showed similar expression as well as similar pigmentation phenotypes. The D. americana tan transgene inserted at 51C had a level of expression between these lines and the line with the transgene inserted at 102D, as well as pigmentation that was intermediate between these lines and 102D. The D. americana tan transgene inserted at 102D had the lowest transgene expression and failed to increase dark pigmentation relative to the tan mutant phenotype.
In summary, by comparing activities of divergent alleles of the same gene at five different genetic locations, we were able to test the assumption that position effects can be ignored as long as the two alleles compared are inserted into the same genomic location and the transgenes are expressed. We found this not to be true; D americana and D. novamexicana tan transgenes inserted at landing site (51C) increased dark pigmentation relative to a D. melanogaster tan mutant, yet showed no significant difference in their relative activity. If we had only compared the effects of these tan alleles at the 51C landing site, we would have concluded that they had conserved functions. The lower level of transgene expression at this site relative to transgenes inserted at the three landing sites that allowed a functional difference between the D. americana and D. novamexicana tan alleles to be detected suggests that landing sites allowing the highest levels of transgene expression might provide the most power for detecting differences between alleles. We recommend that at least three genomic locations should be tested to search for allelic differences in activity. Although this increased production of transgenic lines would increase cost and workload, they would help prevent inaccurate conclusions from being drawn from transgenes affected by position effects.
We would like to thank Scott Barolo and members of the Wittkopp lab for helpful discussions as well as Robert Dikeman for experimental assistance. This work was funded by the National Institutes of Health (R01-GM-089736).
Copyright © 2016 John et al.
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Q: how to integrate essl biometric device data to attendence system through php I want to get the punch in and out time from biometric device and store it in another attendence product database (mysql)

A: download the SDK from zkteco site. and go through the sdk sample programs and documents.
If you are already familiar with the zk program then, You have to read all the users from one device (using ReadAllUserID to buffer the data into device's memory and then GetAllUserInfo) then call SetUserInfo for every user id with another device.
If you are like to do it over http please check http://zkteco.tech/application/biometric-web-api.html for api details.

redpajama_set_name: RedPajamaStackExchange


text:
Optimum Transplanting Date for Rice Production
M. Asghar, M. Sadiq, S. M. Mehdi, M. Sarfraz, G. Hassan and R. Ahmad
Journal of Biological Sciences, 2001, 1(4), 259-260.
The results indicated that maximum yield of paddy was obtained from those plots which were transplanted on 20th July except 1997 where 5th August date gave the maximum but non-significant yield. Among the varieties Shaheen performed better than others. Straw yield decreased with the delay in transplanting irrespective of season. Maximum straw was produced when the lines/varieties were transplanted on 20th June followed by 5th July, 20th July and 5th August in all the years. Among the lines/varieties tested Shaheen produced the least straw. The results indicated that maximum yield of paddy was obtained from those plots which were transplanted on 20th July except 1997 where 5th August date gave the maximum but non-significant yield. Among the varieties Shaheen performed better than others. Straw yield decreased with the delay in transplanting irrespective of season. Maximum straw was produced when the lines/varieties were transplanted on 20th June followed by 5th July, 20th July and 5th August in all the years. Among the lines/varieties tested Shaheen produced the least straw.
ASCI-ID: 38-189


The Cleveland Cavaliers will host the Golden State Warriors on Wednesday night in the NBA.
The Cavs (5-18, 3-8 home) are coming after a rare win, having defeated the Nets in Brooklyn 99-97 a couple of nights ago. Still, that isn`t saying much, as the Cavs remain one of the worst teams in the NBA and wins will be hard to come by this season. With a poor roster, Cleveland has lost 4 of its last 5 games, and wasn`t even competitive in the defeats.
The Warriors (16-9, 5-7 away) have gone though some tough times already this season, with injuries to Curry and Green, as well as the feud between Green and Durant. However, Golden State remains the best team in the league by a mile and they are now on a quest to rack up some wins in a row in order to reclaim the top spot in the Western Conference. The first step was made two nights ago in Atlanta, with the Warriors winning by 17 points.
Bet on Cavs v Warriors and get your 100% (120 Eur) welcome bonus at LSbet now!
Team news: JR Smith and Kevin Love are long term absentees for Cleveland, while Dekker, Nwaba and Zizic have missed the last couple of games and will be unavailable tonight as well (Zizic has a chance to play). The Warriors will continue to be without Green, Cousins, Jones and possibly McKinnie, but the latter might return tonight.
The Warriors have lost some ground to the top teams in the West after struggling the last few weeks without Curry, and now they feel like it`s time to get back down to business and start winning games consecutively.
With Steph back they are already a completely different and much better team, but right now they are also taking games seriously and are motivated to win – maybe more than they usually are in the regular season.
Steph Curry suggested this himself, saying after the Monday Hawks game that the Warriors were keen to start strong and put the game to bed as early as possible in order to get back on track and not leave the door open for Atlanta late.
This will probably be the approach ahead this game against the Cavs as well. And as long as the Warriors are locked in and focused, they should have zero problems against a team like Cleveland.
A similar scenario like in the game against the Hawks could be expected here, and the 11 point handicap should be well within Golden State`s grasp. It`s difficult to see this poor Cavs team staying within touching distance with a Warriors side which is motivated and has Steph Curry back – especially since the Cavs are also dealing with injuries and missing some serious rotation pieces.
The result of my analysis can only be a bet on the Warriors to cover the -11 (asian) handicap. Prediction: Cleveland Cavaliers – Golden State Warriors 100 – 125.


Cute and delicate presentations have no limit! Another super awesome presentation of such themes is found in the Bimba & Lola winter 2013-2014 lookbook, which is finally available for our deep and in-detail scrutiny. The peculiar retro-vintage vibe found in almost all the lines of this luxurious Spanish brand is imprinted in the looks of the new Bimba & Lola collection as well. There you have all the necessary details to create one-of-a-kind combos that will make you stand out in the gray crowd of fall style followers.
What is fresh and quite popular for the nowadays fashion trends is the floral design, found on the majority of Bimba & Lola winter 2013-2014 collection pieces, thus representing the actualized side of the line. This way, in this lookbook we find an interesting cohesive line of spectacular, a bit minimalistic and straight shapes, combined with an interesting succession of colors carrying various color shade compositions like brown tones, combinations of timeless black and white, some navy blue motives, occasionally interrupted with passionate red.
The retro vibe is represented with the nicely cut silhouettes. Thus, the first look of the Bimba & Lola winter 2013-2014 collection features a nice way of introducing the black and white theme, created as floral prints and put all over a pajama-type top and pants combo, having T-shirt cropped shape for the top and some slightly cropped bottom. Going on with the thematic black and white style, a combination of a woolen sweater topping a shirt and paired with pants is found, followed by another spectacular shaping featuring a boatneck top and a straight skirt.
Following this, a variety of coats are presented, which have interpretations of wool motives, some manly oversized shapes, and extended silhouettes. Talking about other compositional patterns, we can't but also mention the nice paisley-resembling florals, boatneck tops, and wool pointed sweaters in navy blue patterns.
The nicely chosen Bimba & Lola accessories are other descriptive elements of the collection. So saying Bimba & Lola winter 2013-2014 lookbook, we mean a nice diversity of looks, put within modernized motives and especially aimed at creating supercool and awesome looks.


Rosa Beltran Design: SOME INSPIRED BATHROOMS FROM ... IKEA??
SOME INSPIRED BATHROOMS FROM ... IKEA??
We're at the "value engineering" stage of our house renovation project right now, which means going over everything with a fine tooth comb to find all the areas where we can save by mixing high/low design without sacrificing anything in style.
Obviously it's no small feat to essentially re-build an entire house (we're tearing down all the interior walls, completely rearranging the existing floor plan, and adding a master suite) and it's no small expense either. Luckily I get a lot of pleasure out of finding creative solutions when designing on a budget. There's something ultra satisfying about combining common things in an unusual and creative way to make it surprising and inspiring!
Lately I've been thinking about the kids' bathroom and wondering what I might be able to do with it using a high/low design approach. I've run across some very attainable ideas from Ikea that I thought you might like too.
I think there are definitely some ideas worth considering here in some way, shape, or form!
We used 2 of the vanities shown in the first picture in our master. They look great (this finish was much better-looking than the gloss white one, we thought), and they are pretty indestructible, with amazing storage. We would absolutely do it again.
I love IKEA as is, but it's inspiring to see their products used in a way that's not obviously IKEA. I have been looking at vanities for a bathroom reno and there are some for over $1000 that are poorly made and look cheap compared to the reasonably priced IKEA ones.
I adore your blog and hope you will make it BIG and get the recognition you deserve! There are so many successful crappy blogs, so I'd love to see a quality blog like yours get the recognition (and financial success) it deserves.
Artluvr this is good to know, thanks!
Very inspiring pictures, I particularly love the 4th, which can be really useful if you miss space !