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Songfest India Launched Xplore, A Music Video IP To promote Independent Musicians
Adding more power to the independent music scene in the country, Songfest India launched the first season of 'Xplore' – a quest to give a platform to some of the most talented indie musicians in India. Announced in August this year, the call for entries for Xplore received an overwhelming response with more than 250 original compositions as entries. Six of the most eclectic tracks will take their music to audiences at large.
An artiste-first music content company, Songfest got together six brilliant artists, to partner with them through the journey of recording the audio, producing the video and distributing them across channels. The platform aims to bridge the gap between indie musicians and access to resources to reach out to the industry. Starting with Sarthak Kalyani's track 'Saahiba', the musical journey of these six talented young artists now begins!
Talking about the experience of providing a platform to independent artists through Xplore, Meghna Mittal, Co-founder and CMO, Songfest India, said, "As an artist-first company, we at Songfest take pride in unveiling Xplore, introducing these six wonderful artists and giving them a platform to showcase their craft to the world. The musicians in the first season of Xplore have been chosen solely based on the strength of their talent and the merit of their compositions as opposed to their fan following, which is usually the case. As creative individuals, we understand the hurdles that independent artists face, and Xplore is a way to provide them support, guidance and help them create music and lend all out strength in sharing it with the wider audience. Our core focus with Xplore is to provide a platform and resources to artists for them to reach their actual potential."
Sarthak Kalyani, the first artist in season 1 of Xplore to have his track released said, "As a musician, all that one wants is to have their music heard by as many people as possible. However, as an independent musician, it is not that easy. Producing a video requires a considerable number of resources to be available at your disposal. But when a platform like Xplore comes your way which gives you all of that and the freedom to create the music you want to, it is quite liberating. It encourages you. It makes you want to create more and give your best because you know that there is an already established audience."
Covid-19Independent MusiciansMeghna MittalSaahibaSongfest
Exclusive Chat With GenZ Digital Content Creator And Independent Indian Hip Hop Artist-Dev Raiyani On His Latest Single 'Wait4me'
Music Licensing Marketplace 'HOOPR' Secures $1.5 Million In Seed Funding LED By 9Unicorns, Venture Catalyst And Inflection Point Ventures


Bad news for outdoors enthusiasts: A new report finds over-the-counter remedies for simple insect bites don't work. In most cases, the best option is to simply tough it out.
The report, published in the Drug and Therapeutics Bulletin, noted mosquitoes, flies, fleas and bedbugs account for most insects bites. The saliva they inject can cause a reaction, an infection, an eczema flare-up, or anaphylactic shock in the most serious cases -- and these warrant appropriate treatment, said the DTB report.
But in most cases, the reaction is mild, and the best course of action is not to bother with over-the-counter remedies for itching, pain and swelling.
"Although antihistamine tablets are widely recommended to quell the itching associated with insect bites, there's not much evidence to back this up," the DTB report. Steroids -- creams and tablets -- are recommended for itching and inflammation, but there is no evidence to support their use, except for people with eczema, according to the report.
Creams containing painkillers/anesthetics, such as lidocaine, benzocaine, or combined with antihistamines and antiseptics, are only "marginally effective and occasionally cause sensitization," the DTB said.
"There is little evidence for the efficacy of treatments for simple insect bites. The symptoms are often self-limiting and in many cases, no treatment may be needed," concludes the DTB.
Most common treatments for insect bites are ineffective and don't do what the labels promise.


Bank of the James' safe deposit boxes offer you the safety and protection you simply can't match in your home. Your valuables are easily accessible in a facility that offers complete privacy. Safe deposit boxes are available for annual rental at all branch offices and in a variety of sizes and prices to accommodate your particular needs subject to availability.


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Simulium tokarense är en tvåvingeart som beskrevs av Takaoka 1973. Simulium tokarense ingår i släktet Simulium och familjen knott. Inga underarter finns listade i Catalogue of Life.

Källor

Externa länkar

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redpajama_set_name: RedPajamaWikipedia


I'm in a Book Club that meets quarterly in Pudsey. We don't take ourselves too seriously, we enjoy reading and wine together. I love it because it makes me read books I wouldn't ordinarily choose to read.
I wouldn't normally describe myself as a fan of crime fiction (although having said that, some of my favourite books this year have been crime fiction – namely the Kate Atkinson Jackson Brodie series!) but this book was just unputdownable!
Ignore the unhelpful title which I don't think does any justice to the story (I don't think it's the same title in Swedish), this is brilliantly written. With multiple plotlines and layers, all weaving together to create the most fantastic literary puzzle, believe the hype, this novel is well worth reading. It's perfect holiday reading and also for commuting – which is when I've read it! The only problem with that is that I've wanted to stay on the bus rather than get off at my stop to go to work!
I won't say any more as I wouldn't want to spoil it for you but all I can say is that as soon as I finished this one I started the second in the trilogy – The Girl Who Played With Fire – immediately. Chain reading I think it's called!


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POLITICS US CONSERVATIVE NEWS
Official! These 20 States Are Suing Trump Over His Latest Obamacare Move! Is Your State On The List?
October 14, 2017 Alex D. 853 Views 2 Comments # Donald Trump, Obamacare
With the failure by Republicans in Congress to repeal Obamacare this year, President Trump is taking matters into his own hands, signing an executive order Thursday that targets the health care law.
Trump is asking federal agencies to look for ways to expand the use of association health plans, groups of small businesses that pool together to buy health insurance, and to broaden the definition of short-term insurance, which is exempt from the Affordable Care Act's rules, administration officials said.
The president was joined at the signing by several Republican lawmakers, including Kentucky Sen. Rand Paul, who called it "the biggest free market reform of health care in a generation."
"This reform, if it works and goes as planned, will allow millions of people to get insurance across state lines at an inexpensive price," Paul said.
It seems that this move made by the President was not welcomed by some liberal attorney general from 20 states!
Nearly 20 states have filed a lawsuit against President Trump over his decision earlier this week to stop key payments that lower health insurance deductibles and co-pays for millions of Americans with modest incomes.
Attorneys general from California, Connecticut, Kentucky, Massachusetts and New York are among the states that announced they had filed the lawsuit in federal court in California Friday. Mr. Trump said Thursday he would end the cost-sharing subsidies, which are given to insurance companies to keep premiums down for lower-income Americans. The states' attorneys general says Mr. Trump is not following federal law in ending a legally mandated system that already is operating.
This is the desperate call from the liberals to stop President Trump!
Alex D is a conservative journalist, who covers all issues of importance for conservatives. He brings attention and insight from what happens in the White House to the streets of American towns, because it all has an impact on our future, and the country left for our children. Exposing the truth is his ultimate goal, mixed with wit where it's appropriate, and feels that journalism shouldn't be censored. Join him & let's spread the good word!
http://www.usasupreme.com/
← NFL Issued A New Ruling On The National Anthem Protests…You Will Not Like This One
BREAKING! Sessions Makes Shocking "Sanctuary City" Announcement (Video) →
Alex D is a conservative journalist, who covers all issues of importance for conservatives. He brings attention and insight from what happens in the White House to the streets of American towns, because it all has an impact on our future, and the country left for our children. Exposing the truth is his ultimate goal, mixed with wit where it's appropriate, and feels that journalism shouldn't be censored. Join him & let's spread the good word!
Sean Hannity Drops Bombshell About Trump's White House
November 15, 2016 Alex D. 2
Trump Signed Legislation Gives States Right To Defund Planned Parenthood
April 13, 2017 Natalie D. 0
Left Wing Comedian Sarah Silverman: "Show Us Your F****** Taxes, You Emotional Child"
2 thoughts on "Official! These 20 States Are Suing Trump Over His Latest Obamacare Move! Is Your State On The List?"
Cecelia Henderson
Nope, Texas won't be on this list. Can anyone tell me why the government is lowering deductibles for millions of Americans, while the rest of them have to carry the full load? This isn't healthcare, this is welfare.
These states have idiots runn8ng them. The Obamacare funding was illegal. For Trump to continue funding it would be illegal. For once we have a POTUS obeying the law. That's the problem with Democrats they think laws don't apply to them. The truth of the matter is that Obama should be up on charges, convicted, and sent to prison. He also committed treason. If we had enough TRUE Republicans and not RINOS in office they would see it done.
Even Lou Dobbs agrees with him.
WATCH: Lou Dobbs Exposes Obama's Treason On National Television –
"Barack Obama, who many claim will be creating a shadow government to frustrate the policy goals of this administration, we're looking at something that's coming very close to, it seems to me, sedition."
VIDEO: https://youtu.be/KgkRpdgP2tI
https://www.teaparty.org/watch-lou-dobbs-exposes-obamas-treason-national-television-2-264878/

redpajama_set_name: RedPajamaCommonCrawl


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The Paw Print 47:1
Turf Fields to Take Root in Stadium Next Fall
Christina Kochanski|May 23, 2013
Turf fields have already replaced most practice fields in Howard County. Photo by Christina Kochanski
Construction will begin on a turf field for Wilde Lake this summer, according to Activities and Athletics Manager Mr. Parnell. Football, soccer, lacrosse, and field hockey teams will be able to play on the field in September, barring any construction problems.
Long Reach and Oakland Mills will also be receiving turf fields this year, reflecting a growing trend that began with Atholton, Hammond, and Howard last year. According to Mr. Parnell, Howard County is giving turf fields to three schools every year until all 12 have replaced their grass fields.
Junior Sarah Hulit has played Varsity soccer for Wilde Lake since her freshman year. She believes that the turf field will be a huge improvement over the current stadium field.
"I'm really excited because playing on a turf field is a lot better than playing on a bumpy, uneven grass field . . . The outcome of every game will be based only on which team is better, not which team gets a lucky or unlucky bounce," said Hulit.
Junior and football player Tyrone Hayward believes that having a turf field can impact football teams as well as soccer teams.
According to Hayward, "It depends on whether the team is a running or passing team. It's good for a running team because when it rains the players won't have to run in the mud." The turf field will also eliminate rain cancellations and will offer an extra practice field for Wilde Lake's teams.
Senior Alex Broholm, who has played soccer for Wilde Lake for four years, believes that playing on a grass field is sometimes advantageous over playing on turf.
"Playing on turf is a different game. It's a lot faster and some teams aren't trained for that. You have an advantage on turf if you're playing a slow team, but if you're playing a physical team grass can be better because you won't get scraped up by the turf," said Broholm.
Mr. Robin Sawyer played soccer at the University of London and James Madison University. He has coached various teams, including Wilde Lake's Varsity Girl's soccer team. According to Mr. Sawyer, playing well on turf often requires changing a team's style of play.
"The bounce is higher. Turf is fast, faster than it needs to be and you can't string passes together . . . Teams have to train on the turf field in order to play well on it," said Mr. Sawyer.
Although coaches and players believe playing on turf to be a different game, turf fields are designed to mimic natural grass. Turf consists of a layer of rubber padding underneath man-made grass blades, according to the Synthetic Turf Council. Small granules of recycled tires known as "crumb rubber" fill the gaps between the grass blades. A drainage layer is built underneath of the padding so fields can be flat without having to worry about flooding.
The benefits to players associated with turf are not without cost. Each field costs about one million dollars, according to Mr. Parnell. The county will also have to pay to maintain the fields.
"The costs are a trade-off because the county won't have to pay to paint or cut the grass anymore," said Mr. Parnell. "Parks and Recreation will also have 12 more fields available for Howard County citizens. Each one will be a park just like Centennial Lane. Youth football, soccer, and lacrosse teams can use the fields through Parks and Rec."
Coach Harrison, currently the head Varsity football coach, has been coaching at Wilde Lake since 1986. He believes that, in addition to financial costs, turf fields can add heat problems.
"Turf gets really hot in summer and early fall. It's one of the drawbacks. In our opening game against Atholton last year, we played on turf and we had a lot of kids cramping up because it was so hot," said Coach Harrison.
Although there are costs and problems associated with turf fields, Mr. Sawyer believes that Howard County's replacement of traditional grass fields with turf reflects a permanent and expansive change in the game of soccer.
"When I grew up I never played on turf, but now kids are playing sometimes exclusively on turf. The gap appears to be generational," said Mr. Sawyer. "If schools continue to replace grass with turf, than grass fields will soon be a thing of the past."
One Response to "Turf Fields to Take Root in Stadium Next Fall"
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As HCPSS' Winter Sports Season is Postponed, Players Prepare for Another Disappointing Season
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Soccer Victories Mean More Than Meet The Eye
Friday Night Games Shine Light on School Pride
Boys Varsity Basketball Team Looks to Continue Early Season Success
Boys Soccer Wins First State Championship Since 1998 in Overtime
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Wilde Lake Boys and Girls Soccer Bring Home Two State Semi Final Victories: Both Teams Advance to 3A Championship
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Asteroid designated 2018 VJ10, first observed at Palomar Observatory in California (ZTF) on November 15, flew past Earth at a distance of 0.52 LD / 0.00133 AU (198 965 km / 123 631 miles) on November 14, 2018.
2018 VJ10 belongs to the Apollo group of asteroids. Its estimated diameter is between 5 and 11 m (16 and 36 feet).
The asteroid flew past us at 20:39 UTC at a speed (relative to the Earth) of 13.50 km/s.
This is the 62nd known asteroid to flyby Earth within 1 lunar distance since the start of the year and 6th this month.


TRX trainer expert Jay Cardiello has an excellent 15 minute TRX workout for your core that will give you stronger, leaner abs. These exercises are perfect for any level and will give your mid-section a very good burn and fire up your core. TRX is the only workout equipment that engages all your muscles at the same time which results in an all round stronger physique.
Perform these exercises for 60 seconds each with a 30 seconds rest in between. Repeat again one more time.
For more suspension training exercises like this, check out Dan Long's Suspension Revolution here.
Be the first to comment on "15 Minute TRX Core Workout"


In recent years, recombinant DNA technology has been used to design insulin molecules that overcome the limitations of regular insulin in mealtime supplementation. However, safety issues have been raised with these alternatives, as the alteration of the three-dimensional structure may alter the interaction with the insulin and/or IGF-I receptors and therefore lead to the activation of alternate metabolic as well as mitogenic signaling pathways. It is therefore essential to carefully study acute and long-term effects in a preclinical state, as insulin therapy is meant to be a lifelong treatment. In this study, we determined in vivo the insulin receptor signaling characteristics activated by insulin glulisine (LysB3, GluB29) at the level of insulin receptor phosphorylation, insulin receptor substrate phosphorylation, and downstream signaling elements such as phosphatidylinositol (PI) 3-kinase, AKT, and mitogen-activated protein kinase. C57BL/6 mice were injected with insulin glulisine or regular insulin and Western blot analysis was performed for liver and muscle tissue. The extent and time course of insulin receptor phosphorylation and activation of downstream signaling elements after insulin glulisine treatment was similar to that of human regular insulin in vivo. Moreover, insulin signaling in hypothalamic tissue determined by PI 3-kinase activity was comparable. Therefore, insulin glulisine may be a useful tool for diabetes treatment.
The introduction of new short- and long-acting insulin and the development of analogs with increased stability, less variability, and selective action will allow more individualized treatment strategies targeted to specific needs. Moreover, the use of these agents is expected to improve glycemic control in type 1 and type 2 diabetic patients (1,2). Recently, a number of insulin variants generated by genetic engineering were found to retard and stabilize absorption kinetics of insulin preparations. However, the altered amino acid sequence of human insulin carries the risk of altered interaction with the insulin and/or IGF-I receptors, which might lead to the activation of different pathways and potentially increase mitogenic activity (3).
Insulin and IGF-I are known to regulate a variety of growth-related and metabolic effects in target tissues. Although predominant activation of the IGF-I receptor correlates with increased mitogenic potency, stimulation of the insulin receptor is mainly associated with enhanced glucose transport, glycogen synthesis, and inhibition of hepatic gluconeogenesis as long as insulin receptor binding kinetics remain normal (3,4).
Insulin binds to its cell surface receptor and stimulates autophosphorylation of the β-subunit, followed by phosphorylation of substrates, including the insulin receptor substrate (IRS) protein family members. The IRS protein family consists of at least six members, IRS1−IRS6. Each IRS protein contains a highly conserved NH2-terminal pleckstrin homology domain followed by a phosphotyrosine binding domain, which couples IRS proteins to the activated insulin or IGF-I receptor (5). After being phosphorylated by the receptor kinase, IRS proteins bind to effector proteins such as the regulatory subunit of the lipid kinase phosphatidylinositol (PI) 3-kinase. Its product, PI phosphate, activates a network of kinases such as AKT and atypical protein kinase C that are involved in processes like glucose transport, protein synthesis, and gene transcription (6).
With the recombinant human insulin analog insulin glulisine (LysB3, GluB29), the tendency to form dimers and hexamers is significantly reduced in subcutaneous adipose tissue due to genetic engineering, leading to a more rapid onset and shorter duration of action than human regular insulin. Recent in vitro data suggest that the novel insulin analog predominantly activates the IRS-2 signaling pathway, which raises a safety concern, as IRS-2 is known to be involved in cell proliferation and mitogenic signaling (7).
Here, we provide in vivo data on the impact of insulin glulisine on the insulin signaling cascade in peripheral tissues. C57BL/6 mice were injected with regular insulin or the analog insulin glulisine; insulin receptor phosphorylation and activation of downstream signaling elements were then analyzed in muscle, liver, and hypothalamic tissue. [3H]thymidine incorporation rates were also determined in C2C12 myoblasts. The resultant data suggest that activation of the insulin signaling cascade is not altered in mice stimulated with insulin glulisine compared with mice treated with regular human insulin, and that the mitogenic potential of insulin glulisine is comparable with that of regular human insulin.
We obtained 10-week-old male C57BL/6 mice from Charles River and allowed them to acclimatize for 2 weeks. They were maintained on a normal light/dark cycle and kept on a regular diet. Glucose levels were sampled from mouse tail bleeds using a Glucometer Elite (Bayer, Elkhart, IN). All procedures were approved by the local Animal Care and Use Committee.
In vivo insulin stimulation and Western blot analysis.
For in vivo stimulation, a bolus of regular human insulin or insulin glulisine (1 IU/kg body wt) was injected intraperitoneally. For short-term stimulation, 2 IU of insulin were injected into the inferior vena cava. Control animals received a comparable amount of diluent. Tissues (liver, muscle, and hypothalamus) were removed at the indicated time points and homogenized at 4°C, as previously described (8). Homogenates were allowed to solubilize for 30 min on ice and were clarified by centrifugation at 12,000g for 20 min. To detect insulin-stimulated tyrosine phosphorylation, supernatants containing 0.5 mg of total protein were immunoprecipitated with antibodies directed against the COOH-terminal of the insulin receptor (KKNGRILTLPRSNPS), IRS-1 (QHLRLSSSSGRLRY), and IRS-2 (QSQPQPGDKNSWSRTR). Visualization of immunocomplexes after gel electrophoresis and Western blotting with the antiphosphotyrosine antibody 4G10 was performed with the nonradioactive enhanced chemiluminescence system. Blots were subsequently stripped and reprobed to reveal expression of total protein. To assess activation of downstream signaling elements, tissue lysates were separated by SDS-PAGE and transferred to nitrocellulose. Membranes were probed with anti–phospho-AKT and anti–phospho−mitogen-activated protein (MAP) kinase antibodies (Cell Signaling, Beverly, MA), respectively. The insulin used in this study was a gift from Aventis Pharmaceuticals.
Assay of PI 3-kinase activity.
Tissue lysates were immunopurified with anti-PY20 (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies and immunocomplexes were absorbed to protein a-sepharose for 12 h. Immunoprecipitates were washed three times and pellets were directly incubated with 0.1 mg/ml l-α-PI (Sigma) and 50 μmol/l [γ32P]-ATP (Perkin Elmer) at room temperature for 10 min. After 150 μl of 1 mol/l HCl was added, lipids were extracted twice with 450 μl chloroform/methanol (1:1 by vol). Products were separated by thin-layer chromatography, as previously described (9). 32P-labeled phospholipids were detected by autoradiography.
[3H]thymidine incorporation into C2C12 myoblasts.
To measure [3H]thymidine incorporation, C2C12 cells were grown in Dulbecco's modified Eagle's medium (4.5 g/l glucose and 10% FCS) in six-well culture plates, and subsequently starved for 24 h in serum-free medium. After cells were stimulated with insulin for 16 h, [3H]thymidine (0.5 μCi/ml) was added for 4 h. The dishes were then rinsed twice with ice-cold PBS and once with 10% trichloroacetic acid. After 20 min, dishes were washed once with ice-cold 10% trichloroacetic acid. Cells were then lysed with 500 μl of 0.2 N NaOH/1% SDS and neutralized with 0.5 ml of 0.2 N HCl. Radioactivity was determined by liquid scintillation counting.
Statistical analyses were done with a two-sided unpaired Student's t test. Data are expressed as means ± SE.
Autophosphorylation of the insulin receptor after stimulation with insulin glulisine in vivo.
Evidence that activation of the insulin/IGF-I signaling pathway is a pivotal element in cell proliferation and induction of mitogenic activity has been provided by in vitro and in vivo studies. To determine whether rapid insulin receptor activation by insulin glulisine differs in terms of the kinetic and/or extent, 12-week-old C57BL/6 male mice were stimulated with a bolus insulin injection into the inferior vena cava. Tissue was dissected after 10 min, and Western blot analysis was performed for liver and muscle tissue. Insulin receptor phosphorylation was markedly enhanced in both muscle and liver 10 min after injection (Fig. 1A), but was comparable with that in animals treated with regular insulin or insulin glulisine, suggesting an equal insulin receptor autophosphorylation kinetic. Looking at early downstream signaling elements, an equal increase in tyrosine phosphorylation of IRS-1 and -2 was observed (Fig. 1B and C).
To test an extended and more physiological response, including regulation of downstream insulin receptor signaling elements, we further injected mice intraperitoneally with regular insulin or insulin glulisine (1 IU/kg body wt). After the injection, there was no difference in the blood glucose−lowering effect between the two insulins. Blood glucose levels dropped significantly from 110 ± 15 to 60 ± 10 mg/dl with regular insulin and to 74 ± 16 mg/dl with insulin glulisine by 20 min (control versus regular insulin or insulin glulisine: P < 0.001) and down to 53 ± 20 mg/dl with regular insulin and 63 ± 7 mg/dl with insulin glulisine by 30 min (control versus regular insulin or insulin glulisine: P < 0.001). There was no significant difference in the blood glucose−lowering effect between the two insulins at each time point (P > 0.1). Moreover, significant changes in blood glucose levels could not be detected 10 min after injection (P = 0.2). Therefore, later time points were analyzed in the further experiments to test downstream insulin receptor signaling pathways.
Looking at insulin receptor phosphorylation, we observed identical results in mice stimulated intraperitoneally with regular insulin or insulin glulisine in muscle (Fig. 2A) and liver (Fig. 2B) tissue. These data suggest that the amino acid exchange of insulin glulisine does not alter insulin receptor activation. Moreover, tyrosine phosphorylation of the insulin receptor was already upregulated after 10 min in muscle and liver (data not shown), suggesting that the phosphorylation kinetics observed in this study were not dependent on the administration route.
Effect of insulin on tyrosine phosphorylation of IRSs in muscle and liver tissue.
After insulin stimulation, IRS-1 is readily tyrosine phosphorylated by the activated insulin receptor. To determine whether activation of IRS-1 is altered after stimulation with insulin glulisine, the tyrosine phosphorylation state of the IRS-1 protein was evaluated in mice treated with insulin. In contrast to data obtained from myoblasts in vitro (7), IRS-1 tyrosine phosphorylation in muscle (Fig. 3A) and liver (Fig. 3B) was comparable for the two groups throughout the 30-min experiment.
Recent studies have revealed that insulin glulisine predominantly activates the IRS-2−dependent pathway (7,10). Because enhanced IRS-2 signaling has been proposed to be involved in mitogenic signal transduction, the phosphorylation state of IRS-2 was determined in vivo in lysates of muscle and liver after insulin stimulation. Mice were stimulated with insulin and tissue was harvested after 20 and 30 min, respectively. Phosphorylation of IRS-2 in muscle (Fig. 4A) and liver (Fig. 4B) was detected by Western blot analysis using an anti-phosphotyrosine antibody. Concurrent immunoblots revealed a comparable increase in IRS-2 phosphorylation for both insulins throughout the experiment.
Activation of PI 3-kinase in muscle and liver tissue after insulin stimulation.
To test IGF-I−like activities, PI 3-kinase activation was determined in mouse liver and muscle after mice were stimulated with insulin glulisine and compared with activation with regular insulin. Tissues were removed 5 and 10 min after insulin injection, and a PI 3-kinase assay was performed. The data suggest that the activation levels in muscle (Fig. 5A) and liver (Fig. 5B) from mice treated with insulin glulisine are equal to those stimulated with regular insulin.
Effect of insulin on the phosphorylation of AKT and MAP kinase in vivo.
Looking at downstream signaling elements, the serine/threonine kinase AKT has emerged as a focal point for many signal transduction pathways regulating GLUT4 translocation, glycogen synthesis, lipogenesis, and gene expression. Growth factor−mediated activation of PI 3-kinase leads to the generation of PtdIns-3,4,5-P3, which recruits inactive protein kinase B from the cytosol to the plasma membrane. Ser473 is then phosphorylated, indicating activation (6). Treatment of mice with insulin led to an activation of AKT in muscle (Fig. 6A) and liver (Fig. 6B). Quantification of five independent experiments revealed a lower phosphorylation for insulin glulisine than for regular insulin after 20 min, but there was no significant difference between the two insulins after 30 min. Moreover, an equal activation of MAP kinase could be observed in muscle (Fig. 7A) and liver (Fig. 7B) tissue by intraperitoneal injection of regular insulin and insulin glulisine.
Looking at mitogenic activity, [3H]thymidine incorporation rates in C2C12 myoblasts stimulated with increasing concentrations of regular insulin or insulin glulisine were determined. Insulin glulisine stimulated [3H]thymidine incorporation to the same extent as regular insulin, suggesting a comparable mitogenic potential (Fig. 7C).
Activation of PI 3-kinase in hypothalamic tissue after insulin stimulation.
Recent data suggest that insulin signaling in the brain is important in maintaining body weight regulation, as abnormalities in hypothalamic signaling lead to hyperphagia and weight gain (11). We therefore assayed the activation of the insulin signaling cascade in the hypothalamus of mice treated with an acute intravenous dose of regular insulin or insulin glulisine. The results obtained indicate that the regulation of the early insulin signaling cascade was equivalent between the two groups, suggesting intact insulin signaling in the central nervous system (Fig. 8).
Modifications of the amino acid sequence of the insulin molecule have resulted in prandial analogs with pharmacokinetic characteristics that more closely mimic the physiological insulin response to a meal after injection (12,13). Moreover, basal day-long glycemic control can be reached with new long-acting analogs that show distinguished absorption kinetics (14).
So far, >300 insulin analogs have been produced, but most of them have been removed from use due to safety concerns after careful in vitro and in vivo examination (15). Given that insulin treatment is most often required over a patient's entire life, it is mandatory to extensively determine potential side effects in a preclinical state, as alteration of the three-dimensional structure affects insulin receptor activation and may lead to altered metabolic as well as mitogenic signaling (3). Insulin glulisine is a new rapid-acting insulin intended for mealtime insulin delivery via subcutaneous injection or external infusion pump. The pharmacodynamics of insulin glulisine are similar to those of insulin lispro and insulin aspart in that insulin glulisine display a more rapid onset and shorter duration of action than regular human insulin due to the lack of hexamere formation.
We earlier tested insulin receptor binding characteristics and receptor auto- and dephosphorylation kinetics of insulin glulisine in rat-1 fibroblasts overexpressing the human insulin receptor and found no alterations compared with regular human insulin (16). However, additional in vitro data accumulated over the past year suggest that insulin glulisine differs slightly in terms of downstream activation of the insulin signaling cascade. In rat cardiomyocytes, a prominent phosphorylation of IRS-2 has been described, with only marginal IRS-1 phosphorylation (7). Comparable results were obtained for human skeletal muscle cells incubated with an extensive amount of insulin glulisine (7). Moreover, an inhibition of cytokine and fatty acid−induced apoptosis was described in a rat insulinoma cell line through activation of IRS-2 and was favored as an antiapoptotic activity of insulin glulisine, thereby opening a new field for optimized insulin treatment (10). However, these data also raise a safety concern, as IRS-2 is involved in cell proliferation and mitogenic signaling. On the other hand, in vitro studies in murine and human cell lines have never revealed changes in cell proliferation due to the incubation with insulin glulisine, even when high concentrations of insulin have been used (17).
Here we report for the first time on in vivo data for insulin glulisine from C57BL/6 mice that were treated with physiological dosages of either regular insulin or insulin glulisine. To overcome the effect of subcutaneous absorption kinetics and focus on direct insulin receptor signaling characteristics, insulin administration in this study occurred by injection in the inferior vena cava or intraperitoneally.
In our in vivo experiments, insulin glulisine activated the insulin receptor as well as insulin receptor substrates to the same extent as regular human insulin. Moreover, activation of PI 3-kinase and downstream signaling elements was equal in liver, muscle, and hypothalamic tissue, suggesting comparable in vivo activation of the insulin signaling cascade. These results are in contrast to the in vitro data obtained from myoblasts, cardiomyocytes, human skeletal muscle cells, and an insulinoma cell line (7,10), most likely due to the extensive amount of insulin and the nature of the cell lines used in those studies.
The current in vivo results support the use of the novel insulin analog insulin glulisine as a rapid-acting insulin analog with unaltered insulin receptor signaling characteristics.
Phosphorylation kinetics of the insulin receptor, IRS-1, and IRS-2 in vivo. Random-fed C57BL/6 mice were injected with human regular insulin or insulin glulisine into the inferior vena cava. Tissues were harvested after 10 min, and tyrosine phosphorylation was measured by immunoblotting in insulin receptor (A), IRS-1 (B), and IRS-2 (C) immunoprecipitates from muscle (left) and liver (right) tissue. Quantification of phosphorylation kinetics by scanning densitometry normalized to the total amount of protein is given below the immunoblots. Scanning data obtained from five independent experiments are expressed as fold increase over basal (untreated) phosphorylation ± SE.
Autophosphorylation kinetics of the insulin receptor in vivo. C57BL/6 mice (age 12 weeks) were injected intraperitoneally with human regular insulin or insulin glulisine. Tissues were harvested at the indicated time points, and tyrosine phosphorylation was measured by specific immunoblotting in insulin receptor immunoprecipitates from muscle (A) and liver (B) tissue. Quantification of receptor autophosphorylation kinetics by scanning densitometry normalized to the total amount of insulin receptor protein is given below immunoblots. Scanning data obtained from five independent experiments are expressed as fold increase over basal (untreated) phosphorylation ± SE.
Phosphorylation kinetic of IRS-1. Random-fed C57BL/6 mice were injected intraperitoneally with human regular insulin or insulin glulisine in a dosage of 1 IU/kg body wt. Tissues were harvested at the indicated time points, and tyrosine phosphorylation was measured by specific immunoblotting in IRS-1 immunoprecipitates from muscle (A) and liver (B). Quantification of IRS-1 phosphorylation kinetics normalized to the total amount of IRS-1 protein by scanning densitometry are given below immunoblots. Scanning data obtained from five independent experiments are expressed as fold increase over basal (untreated) phosphorylation ± SE.
Phosphorylation kinetic of IRS-2. C57BL/6 mice (age 12 weeks) were injected intraperitoneally with human regular insulin or insulin glulisine. Tissues were harvested at the indicated time points, and tyrosine phosphorylation was measured by specific immunoblotting in IRS-2 immunoprecipitates of 500 μg total protein from muscle (A) and liver (B). Quantification of IRS-2 phosphorylation kinetics by scanning densitometry normalized to the total amount of IRS-2 protein are given below immunoblots. Scanning data obtained from five independent experiments are expressed as fold increase over basal (untreated) phosphorylation ± SE.
Effect of insulin glulisine and human regular insulin on PI 3-kinase activity in muscle and liver. C57BL/6 mice (age 12 weeks) were injected into the inferior vena cava with human regular insulin or insulin glulisine in a total dosage of 2 IU. Muscle (A) and liver (B) tissue was harvested at the indicated time points, lysates were immunoprecipitated with PY20 antibodies, and an in vitro kinase assay was performed using l-α-PI as a substrate. Scanning data obtained from three independent experiments are expressed as fold increase over basal (untreated) ± SE.
Phosphorylation of Ser-473 AKT. C57BL/6 mice (age 12 weeks) were injected intraperitoneally with human regular insulin or insulin glulisine. Tissues were harvested at the indicated time points, and Ser-473 phosphorylation of AKT was measured by specific immunoblotting in muscle (A) and liver (B). Quantification of kinetics by scanning densitometry normalized to the total amount of AKT protein are given below immunoblots. Scanning data obtained from five independent experiments are expressed as fold increase over basal (untreated) phosphorylation ± SE.
Phosphorylation of MAP kinase in vivo and [3H]thymidine incorporation into C2C12 cells. C57BL/6 mice (age 12 weeks) were injected intraperitoneally with human regular insulin or insulin glulisine. Tissues were harvested at the indicated time points, and phosphorylation of MAP kinase was measured by specific immunoblotting in muscle (A) and liver (B). Equal protein loads were ensured using an anti-elF4E antibody. Two representative immunoblots are shown (n = 5). C: C2C12 myoblasts were incubated with regular insulin or insulin glulisine at the indicated concentrations for 16 h. Results are means of four experiments performed in duplicate, and expressed as the percentage of maximal response seen in the presence of 10% FCS (100%) ± SE.
Effect of insulin glulisine and human regular insulin on PI 3-kinase activity in hypothalamic tissue. C57BL/6 (age 12 weeks) mice were injected into the inferior vena cava with human regular insulin or insulin glulisine in a total dosage of 2 IU. Hypothalamic tissue was harvested after 10 min. Lysates were immunoprecipitated with anti-PY20 antibodies and an in vitro kinase assay was performed using l-α-PI as a substrate. Scanning data obtained from three independent experiments are expressed as fold increase over basal (untreated) ± SE.
We are grateful to Katrin Brodbeck and Roman Werner for their excellent technical assistance.
H.-U.H. is a member of an advisory panel for and has received honoraria from Aventis Pharma Deutschland.


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HomeAnime News Archives - Anime CornerBocchi the Rock Releases Episode 9 Preview
Bocchi the Rock Releases Episode 9 Preview
Anime Tv November 29, 2022
The official Twitter of Bocchi the Rock has released preview images for episode 9. Bocchi is acting strange lately due to not being invited by anyone from the band to any outing activities all summer long. In order to bring their friend back to normal, the group has decided to take a trip to Enoshima island. Don't miss out on their upcoming excursion in episode 9 of the series which airs on Saturday, December 3.
Bocchi the Rock – Episode 9 Preview Images
Yoshihiro Hiramine (This Art Club Has a Problem!) is directing and handling the storyboard of the upcoming episode while Hiroki Ito (My Dress-Up Darling episodes 2, 6, & 12) is the animation director. Studio CloverWorks is animating the anime which is based on a 4-Koma manga by Aki Hamaji. Erika Yoshida is writing the scripts and Kikuya Tomoki is composing the music.
Bocchi the Rock Manga Author Draws Bocchi for Anime Episode 8
Crunchyroll is streaming the anime and describes the plot as:
Hitori Gotoh, "Bocchi-chan," is a girl who's so introverted and shy around people that she'd always start her conversations with "Ah…" During her middle school years, she started playing the guitar, wanting to join a band because she thought it could be an opportunity for even someone shy like her to also shine. But because she had no friends, she ended up practicing guitar for six hours every day all by herself. After becoming a skilled guitar player, she uploaded videos of herself playing the guitar to the internet under the name "Guitar Hero" and fantasized about performing at her school's cultural festival concert. But not only could she not find any bandmates, before she knew it, she was in high school and still wasn't able to make a single friend! She was really close to becoming a shut-in, but one day, Nijika Ijichi, the drummer in Kessoku Band, reached out to her. And because of that, her everyday life started to change little by little…
© Aki Hamaji / Hobunsha, Aniplex
The post Bocchi the Rock Releases Episode 9 Preview appeared first on Anime Corner.
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At least 10 killed and dozens injured in Cambodia casino hotel fire | Cambodia
At least 10 people have died and dozens have been injured in a fire that engulfed a casino hotel in Cambodia late on Wednesday, officials have said.
Multiple videos shared online showed flames erupting from the Grand Diamond City hotel and casino in Poipet, a popular commuter town on the border with Thailand, as hundreds of rescue workers struggled to contain the blaze.
Footage showed the massive complex ablaze, with some clips appearing to show people trapped inside the burning building while others jumped from windows as rescuers tried to reach them.
A provisional police report seen by Agence France-Presse said "about 10 people died and 30 people injured", adding that about 400 individuals were believed to be working at the casino.
Local media reported that foreign nationals were inside the casino at the time of the fire.
Nhem Phoeng, Poipet administrative chief, told the Phnom Penh Post that more than 400 people, most of them Thais, became stuck inside the casino. He said most of the deceased were Cambodian and Thai employees.
Major General Sithi Loh, the police chief in Banteay Meanchey province, said many of the injured were believed to be in a critical condition, adding that 360 emergency personnel and 11 fire trucks were sent to the scene as rescue teams continue to search for victims.
The cause of the fire so far remains unclear.
Thai rescue workers told CNN they responded to emergency calls from Cambodian authorities to help tackle the blaze on the third floor of the hotel. The fire broke out about 11.30pm local time, Cambodian police said.
A Thai regional government official from the adjacent town of Aranyaprathet, which abuts Poipet, said the fire spread to a 17-storey building nearby.
Pruk Chomcheewin Aui Song Sahai, secretary to the town's mayor, uploaded a video of the inferno, purportedly live from the scene. He said authorities were continuing to search for survivors and the deceased.
Rescue workers lower people to the ground as a fire rages at the Grand Diamond City hotel-casino in Poipet, Cambodia. Photograph: AFP
A Thai foreign ministry source also said they were coordinating closely with local authorities, with the injured transferred to hospitals in Thailand's Sa Kaeo province. "The authorities have been trying to control the fire including by sending in firetrucks from the Thai side," they said.
A volunteer with Thai rescue group the Ruamkatanyu Foundation said the blaze started on the first floor but spread quickly along the carpets, leaping up through the multi-storey building.
Thai public broadcaster PBS said that 50 Thai nationals including hotel staff and customers were trapped inside the casino complex.
Parinya Phothisat, the governor of Thailand's Sa Kaeo province said that one of those killed was a Thai national and 32 Thais were being treated in hospital.
Other reports have indicated the number of those injured and taken to hospital is much higher.
Authorities confirmed the fire was extinguished just after 11am local time on Thursday.
The Grand Diamond City is one of a number of casino-hotels clustered along the Thai-Cambodian border.
Agence France-Presse and Reuters contributed to this report
Most of Thailand to become cooler
รู้ตัวแล้ว ฝรั่ง-สาวไทย เซิ้งอุจาดข้างเสาไฟจนเป็นข่าว ลั่นเมา ชีวิตหลุดเข้าไปในมัลติเวิร์ส


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69ers: a summary
Posted on August 30, 2010 by J
I've now completed 69ers. Here's a summary.
The novel is set (predictably enough) in August 1969, at the UK's first great rock festival on the Isle of Wight. Bob Dylan is due to break a long spell of introversion by performing there, just a couple of weeks after the groundbreaking events at Woodstock, near his home, which he chose to avoid. Men have just landed on the moon, Charles Manson's gang are terrorising Hollywood, troops are on their way to Northern Ireland, and Scott Rayner, a 16-year-old grammar school boy and would-be progressive rock star, is setting off over the Solent with his charismatic but abusive pal Gerry to seek work at the festival and make the bootleg tape which will earn them the admiration of their peers.
Life in Woodside Bay is however very different to the cloistered world of the Isaac Watts Grammar School for Boys. The festival proves to be more of a challenge to Scott than he anticipated, especially when he falls for radical army child Jayne, whose commitment to a communal lifestyle draws out a conservative side to Scott he barely realised existed.
Meeting a colourful array of characters from all corners of the world, experimenting with dope and magic mushrooms, celebrating and debating the music and its relevance, Scott is taken on a journey which will change him forever – especially when a disastrous sexual encounter leads to the theft of his prized tape recorder, the end of his friendship with Gerry, and an intensification of his bond with Jayne, who offers to help him face his fears in order to recover his possession.
69ers is a unique take on an unique event in UK history, the issues raised by which still reverberate today. The author, Jon Blake, is the son of the electrical contractor for the festival and has many unique insights arising from his experience there, together with a lifelong interest in music history. He is the author of over fifty books for young people including the critically acclaimed young adult thriller The Last Free Cat.


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I'm happy to say that tenacious amateur sleuth Kate Shackleton is back for her eighth outing. (I wrote about my first encounter with Kate in Whitby here.) This time she's in for some starry encounters, as she scores an unusual invitation to view the 1927 eclipse and is drawn into investigating some dramatic deaths.
Frances Brody captures all the excitement of the 1927 eclipse well and weaves it seamlessly into her story. It's fascinating to see where the Astronomer Royal chooses to view it from in the path of totality and how the author uses that setting so well, bringing in minor as well as the main characters to help us see the relevance to them as well as those who attend the ticketed event. I got a real sense of occasion, the planning which went into it, and its importance to the chosen school. I also enjoyed how breezily Kate manages to arrange a flight up there and back.
The tiny brass bell on the cafe's door rang softly as Geneva let herself in.
Two of her regulars looked up from their seats at the counter: Huxley, a local retiree, and Tim, a long-haul trucker who stayed on a Houston–Chicago route week in and week out. "Sheriff's here," Huxley said as Geneva passed behind him. At the end of the counter, she opened the gate that led to her "main office," the space between the kitchen and her customers. "Rolled in 'bout thirty minutes after you left," he said, both he and Tim craning their necks to gauge her reaction.
"Must have made ninety miles an hour the whole way," Tim said.
Geneva kept her lips pressed together, swallowing a pill of rage.
She lifted an apron from a hook by the door that led to the kitchen. It was an old one, yellow, with two faded roses for pockets.
"Sheriff?" Wendy said from her perch at the other end of the counter. She was sitting in front of a collection of mason jars, each filled with the very best of her garden. Plump red peppers, chopped green tomatoes threaded with cabbage and onion, whole stalks of okra soaked in vinegar. Geneva lifted each jar one by one, holding it up to the light and double-checking the seal.
"I got some other stuff outside," Wendy said as Geneva pulled a marker from the pocket of her apron and started writing a price on the lid of each jar.
"You can leave the chow chow and the pickled okra," Geneva said, "but I got to draw the line on all that other junk you trying to sell." She nodded out the front window to Wendy's car. Wendy and Geneva were the same age, though Wendy had a tendency to adjust her age from year to year depending on her audience or mood. She was a short woman, with mannish shoulders and an affected disregard for her appearance. Her hair was gray and pomaded into a tight bun. At least it had been tight last she combed it, which could have been anywhere from three to seven days ago. She was wearing the bottom half of a yellow pantsuit, a faded Houston Rockets T-shirt, and men's brogues on her feet.


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Sir David Attenborough Celebrated With Newly Discovered Flatworm
Home / News » Sir David Attenborough ...
A team of Czech, Belgian and Austrian researchers from Masaryk University and the University of Graz has discovered two new flatworm species in the waters of Africa's Lake Tanganyika. One of them was coined Cichlidogyrus attenboroughi, after the legendary BBC documentary maker and naturalist, as a present for his 90th birthday.
When discovering new species, scientists are relatively free in the choice of their names. This opportunity may hence be used to honour colleagues and friends, lovers and celebrities. And who better to honour as a biologist than Sir David Attenborough, in gratitude for his contributions to nature education, inspiring millions worldwide?
"The thought had crossed our minds already for some time, but dedicating a species to Sir David seemed especially fitting at the occasion of his 90th birthday" says Nikol Kmentová, a Czech parasitologist who described the new species. Numerous animals and plants have been named after Attenborough, but this seems to be the first flatworm and probably the tiniest organisms named after him.
The newly discovered worms, whose descriptions were published in the specialised journal Parasites & Vectors, live as parasites on the gills of deep-water fishes in the Lake. Despite their minute size (a few tenths of a millimetre long), these modest critters are impressive testimony to what evolution is capable of. Considering that many of these worms are relatively picky as regards their choice of fish to settle on, imagine that they have to find, in this immense body of water, a suitable fish shortly after hatching from their egg, and then remain attached to its gills for the rest of their life…
"The unique ecosystem of Lake Tanganyika is a well-known showcase of how diverse life underwater can be. Studying its deep-water fishes is a particular challenge – hence we mainly worked with fish landed by fishermen for the commercial market" explains Stephan Koblmüller, an Austrian biologist specialising in the Lake's astonishing diversity of fishes.
"Belgian researchers have explored Lake Tanganyika for many years" adds supervisor Maarten Vanhove from our Institute, "often in collaboration with scientists from the neighbouring countries. RBINS in particular has a rich history in scientific research on Lake Tanganyiaka, as it was responsible for the management of the national parks of DR Congo until its independence in 1960. THe archives of scientific missions between 1925 and 1960, including an exploration of the lake in 1946-1947, were recently digitalised by CEBioS.
"By focusing on the Lake's parasite fauna, we want to highlight that apart from the Lake's famous fish fauna, this ancient ecosystem harbours much more biodiversity than meets the eye – also a recurring message in the work of Sir David" Vanhove concludes.
Categories: OD Taxonomy and Phylogeny, Scientific News


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Kicking off in Pittsburgh on March 29.
In spring 2019 the Be The Cowboy tour continues in U.S cities we didn't get to play the first time around! Thanks for waiting :') also WE'RE GOING W @jaysomband ✨ Swipe to see presale and on sale info in the second image!
On Monday, Mitski revealed a new set of dates for the spring edition of her Be The Cowboy tour. The strictly U.S. stops are "cities we didn't get to the first time around," the Be The Cowboy singer said on Instagram. The 17-night run of shows will kick off in Pittsburgh, PA on March 29 and will hit Columbus, Lousiville, Indianapolis, and St. Louis among others. The tour will then close out on April 20 in North Adams, Massachusetts.
Pre-sale for Mitski's spring trek will be available via her website on December 14. Check new dates up above.
Thumbnail image courtesy of Natalia Mantini for The FADER.


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Irene Mayhew Bassett (1872–1959)
John O'Neil, photographer (New Bedford, Massachusetts), Irene Mayhew Bassett, ca. 1890; privately held by the Faulkner-Hull Collection.
Irene Mayhew Basset was born in June 1872 in Massachusetts to William A. Bassett and Almira D. Mayhew.1 During her childhood and teen years, Irene lived with her parents and siblings, William, Charles, Alvah, Edwin, Frederick, and Dorothy at 15 Chestnut in New Bedford and attended Middle Street Grammar School.2
Tragedy struck the family in 1886, when Irene's brothers, Alvah and Eddie, fell through the ice while playing and Eddie did not survive. Eddie is buried in Oak Grove Cemetery in New Bedford.3
"Dead Under Ice, Eddie Bassett," newspaper article, The Boston Globe (Boston, Massachusetts), 17 Jan 1886, p. 1, col. 8.
Irene graduated from New Bedford High School in 1890.4
Annual Report of the School Committee of the City of New Bedford, with the Superintendent's Annual Report, for the Year 1890, (New Bedford : Mercury Publishing Company, 1891), p. 21-23, New Bedford High School Graduating Exercise, 27 June 1890.
After high school, Irene became a kindergarten teacher and lived with her parents and siblings, Charles, Alvah, and Dorothy.5 Irene's father, William A. Bassett, died in 1903.6
"William A. Bassett," death notice, The Fall River Daily Herald (Fall River, Massachusetts), 26 Feb 1902, p. 4, col. 4.
Following her father's death, Irene and all of her siblings except William lived at 166 Mills Street with their mother, Almira, and their mother's siblings, Allen Mayhew and Harriet Mayhew Davis.7 Irene's mother, Almira Mayhew Bassett, died in 1911 and is buried in Oak Grove Cemetery in New Bedford.8 When their uncle, Allen, died in 1917, he left the majority of his estate to Irene and her siblings.9
"Will of A. G. Mayhew," newspaper article, The Fall River Daily Evening News (Fall River, Massachusetts), 6 Sept 1917, p. 7, col. 3.
Irene, Charles, and Alvah lived at 166 Mill Street for decades. Charles and Alvah ran the fish market and Irene continued to teach school. Dorothy remained at the home until the 1920s, about the same time Fred moved back in following the death of his wife, Dora, in 1922.10 In 1932, Irene and Dorothy traveled to Honduras, returning on the S.S. San Benito with a cargo of bananas.11
"Port of Boston, SS San Benito Arrives from Tela, Honduras," newspaper article, The Boston Globe (Boston, Massachusetts), 29 Aug 1932, p. 10, col. 4.
Irene's brother, William, died in Cheshire, New Haven County, Connecticut in 1934. He is buried in Rural Cemetery in New Bedford.12
"William H. Bassett," obituary, The Brooklyn Daily Eagle (Brooklyn, New York), 22 Jul 1934, p. 17, col. 3.
Irene's brothers, Frederick and Alvah, died in 1944 and 1954 respectively.13 Irene and her brother, Charles, remained living together at 166 Mill Street in New Bedford until his death in 1956.14
Irene's sister, Dorothy, was a noted psychologist. She died in Philadelphia, Pennsylvania in 1958 and is buried in Oak Grove Cemetery in New Bedford.15
"Dorothy Bassett, Psychologist Dies at Age 67," obituary, The Daily Journal (Vineland, New Jersey), 28 Jan 1958, p. 2, col. 1.
The last surviving sibling, Irene died at the age of about 87 in 1959 in New Bedford.16
1. Full name fo Irene Mayhew Bassett listed in Annual Report of the School Committee of the City of New Bedford, with the Superintendent's Annual Report, for the Year 1890, (New Bedford : Mercury Publishing Company, 1891, p. 23, New Bedford High School Graduating Class of 1890, Irene Mayhew Bassett.
No birth record found in page-by-page searches, New Bedford, Bristol County, Massachusetts, Births Register 1871, vol. 4, p. 74-94A, 485 births; Family Search.
Also, New Bedford, Bristol County, Massachusetts, Births Register 1872, vol. 4, p. 95-116, 508 births; Family Search.
Also, New Bedford, Bristol County, Massachusetts, Births Register 1873, vol. 4, p. 117-138, 502 births; Family Search.
Also, New Bedford, Bristol County, Massachusetts, Births Register 1874, vol. 5, p. 1-12, 537 births; Family Search.
Also, New Bedford, Bristol County, Massachusetts, Births Register 1875, vol. 5, p. 13-25, 587 births; Family Search.
The 1900 census states a year and month of June 1872 for Irene's birth.
1900 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 187, sheet 15B, dwelling 286, family 371, William A. Basset household.
Address: 83 Summer Street
– Irene M., daughter, age 27, single, born June 1872 in Massachusetts, school teacher
– Dorothy, daughter, age 9, single, born Sept 1890 in Massachusetts
Also, Irene and her sister, Dorothy, traveled to Honduras in 1932 and both of their birth years appear to be 4 years later than the known birth years for both Irene and Dorothy. Dorothy's month and day match her birth record. It is possible Irene's birth was on 21 June 1872.
Passenger Lists of Vessels Arriving at Boston, Massachusetts, 1891-1943, S.S. San Benito, Tela, Honduras to Boston, Massachusetts, departure 22 Aug 1932, arrival 28 Aug 1932, manifest list, p.174, nos. 3, 4, Irene and Dorothy Bassett.
– no. 3, Bassett, Irene, age 56, female, single, birth 21 June 1876.
– no. 4, Bassett, Dorothy, age 41, female, single, birth 4 Sept 1894.
Also, New Bedford, Bristol County, Massachusetts, Births Register 1890, vol. 7, p. 41, no. 648, Dorothy Bassett, 4 Sept 1890. Parents: William A. [Bassett], born in New Bedford, and Almira D. May [unreadable], born in Westport. Father's occupation: Fish dealer.
2. 1880 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 111, page 16, dwelling 149, family 202, William A. Bassett household.
Address: 15 Chestnut
– William A. Bassett, age 36, born in Massachusetts, fish dealer
– Elmira D., wife, age 32, born in Massachusetts
– Wm. H., son, age 12, born in Massachusetts
– Irene M., daughter, age 9, born in Massachusetts
– Charles M., son, age 6, born in Massachusetts
– Alvah H., son, age 3, born in Massachusetts
– Edwin B., son, age 1, born in Massachusetts
Also, New Bedford, Bristol County, Massachusetts, Births Register 1882, vol. 5, p. 118, no. 248, Frederick C. Bassett, 25 May 1882. Parents: William A. [Bassett], born in New Bedford, and Almira Mayhew, born in Westport. Father's occupation: Fish dealer.
Also, W. A Greenough, complier, 1893 The New Bedford and Fairhaven Directory, (Boston, Massachusetts : W. A Greenough, 1893), p. 84, William A. Bassett.
– Bassett, William A., fish, 193 Purchase, h. 15 Chestnut
3. "Dead Under Ice, Eddie Bassett," newspaper article, The Boston Globe (Boston, Massachusetts), 17 Jan 1886, p. 1, col. 8.
Also, New Bedford, Bristol County, Massachusetts, Deaths Register 1886, vol. 5, p. 49, no. 31, Edwin B. Bassett, 16 Jan 1886. Male. Age: 7 years, 1 month. Cause of death: Drowned. Place of death: Chestnut Street. Place of birth: New Bedford. Parents: William A. and Almeda D. Burial: Oak Grove.
Also, Find A Grave, memorial 23341095, Edwin B. "Eddie" Bassett (1879–1886), Oak Grove Cemetery, New Bedford, Bristol County, Massachusetts; no headstone photo.
4. Annual Report of the School Committee of the City of New Bedford, with the Superintendent's Annual Report, for the Year 1890, (New Bedford : Mercury Publishing Company, 1891), p. 21-23, New Bedford High School Graduating Exercise, 27 June 1890, Irene May Bassett
5. W. A Greenough, complier, 1899 New Bedford and Fairhaven City Directory, (Boston, Massachusetts : W. A Greenough, 1899), p. 102-103, Bassett family listings.
– Bassett, Alvah H., mill apprentice, b. 83 Summer
– Bassett, Charles M., clerk, 193 Purchase, b. 83 Summer
– Bassett, Irene M. kindergarten teacher, b. 83 Summer
– Bassett, William A., fish, 193 Purchase, whol. 161 Front, h. 83 Summer
Also, 1900 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 187, sheet 15B, dwelling 286, family 371, William A. Basset household.
– William A. Basset, age 56, married 34 years, born Mar 1844 in Massachusetts, fish dealer
– Almira D., wife, age 52, married 34 years, born May 1848 in Massachusetts, 8 children born, 6 living
– Charles M., son, age 25, single, born Oct 1874 in Massachusetts, fish dealer
– Alvan H., son, age 22, single, born Nov 1877 in Massachusetts, second hand mill
6. New Bedford, Bristol County, Massachusetts, Deaths Register 1902, p. 297, no.185, William A. Bassett, 24 Feb 1902. Age: 57 years, 11 months, 19 days. Place of death and burial: New Bedford. Place of birth: New Bedford. Cause of death: Heart disease. Parents: William C. [Bassett] and Harriet Luther.
Also, "William A. Bassett," death notice, The Fall River Daily Herald (Fall River, Massachusetts), 26 Feb 1902, p. 4, col. 4.
7. 1910 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 199, ward 4, sheet 8A, dwelling 111, family 148, William A. Basset household.
Address: 166 Mill Street
– Almira D., age 60, married 44 years, born in Massachusetts, 7 children born, 6 living
– Irene M., daughter, age 35, single, born in Massachusetts, public school teacher
– Charles M., son, age 34, single, born in Massachusetts, fish market employee
– Alva H., daughter, age 32, single, born in Massachusetts, fish market
– Fred C., son, age 19, single, born in Massachusetts, twist drill
– Dorothy, daughter, age 19, single, born in Massachusetts
– Allen Mayhew, brother-in-law, age 72, widowed, born in Massachusetts
– Harriet Davis, sister-in-law, age 72, widowed, born in Massachusetts
8. Commonwealth of Massachusetts, certificate of death, no. 440, New Bedford, Bristol County, Massachusetts, Almira D. Bassett, 25 Feb 1911. Place of death: 166 Mill St., New Bedford. Maiden name: Mayhew. Widow. Spouse: William A. [Bassett]. Birth: 2 May 1848. Age: 62 years, 9 months, 23 days. Parents: Jeremiah B. [Mayhew] and Dorothy B. Manchester. Cause of death: Hepatitis. Informant: Husband. Burial: Oak Grove Cemetery, 28 Feb 1911.
Also, Find A Grave, memorial 233230949, Almira Durfee Mayhew Bassett (1848–1911), Oak Grove Cemetery, New Bedford, Bristol County, Massachusetts; no headstone photo.
9. "Will of A. G. Mayhew," newspaper article, The Fall River Daily Evening News (Fall River, Massachusetts), 6 Sept 1917, p. 7, col. 3.
– William H. Bassett of Cheshire, Connecticut, watch and chain
– Irene M. Bassett of New Bedford, $1000, niece
– Dorothy M. Bassett of New Bedford, $1000, niece
Residue divided between:
– William H. Basset of Cheshire, Connecticut, nephew
– Fred C. Bassett of Cheshire, Connecticut, nephew
– Alvah H. Bassett of New Bedford, nephew
– Charles M. Bassett of New Bedford, nephew
10. 1920 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 140, ward 4, sheet 3A, dwelling 41, family 58, Irene Basset household.
– Irene Bassett, age 44, single, born in Massachusetts, public school teacher
– Charles, brother, age 45, single, born in Massachusetts, fish dealer, own shop
– Alvah, brother, age 43, single, born in Massachusetts, salesman, fish shop
– Dorothy, sister, age 30, single, born in Massachusetts, public school teacher
Also, 1930 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 125, ward 4, sheet 15A, dwelling 269, family 269, Irene Basset household.
– Fred C., brother, age 46, widower, born in Massachusetts, salesman, fish market
– Alvah H., brother, age 50, single, born in Massachusetts, proprietor, fish market
– Charles M., brother, age 55, single, born in Massachusetts, salesman, fish market
Also, 1940 U.S. census, New Bedford, Bristol County, Massachusetts, population schedule, enumeration district (ED) 20-76, sheet 13B, family 300, Irene Basset household.
– Irene Bassett, age 60, single, born in Massachusetts, supervisor, special classes public schools – Charles M., brother, age 65, single, born in Massachusetts, fish clerk, retail fish store
– Alvah H., brother, age 62, single, born in Massachusetts, owner of fish store
– Fred C., brother, age 56, single, born in Massachusetts, fish clerk, fish market
Also, "Cheshire, Mrs. Fred C. Bassett," funeral notice, Record-Journal (Meriden, Connecticut), 8 Aug 1922, p. 12, col. 6.
Burial location changed to New Bedford, "Cheshire, Mrs. Fred C. Bassett," burial notice, Record-Journal (Meriden, Connecticut), 8 Aug 1922, p. 12, col. 6.
11. "Port of Boston, SS San Benito Arrives from Tela, Honduras," newspaper article, The Boston Globe (Boston, Massachusetts), 29 Aug 1932, p. 10, col. 4.
Also, Passenger Lists of Vessels Arriving at Boston, Massachusetts, 1891-1943, S.S. San Benito, Tela, Honduras to Boston, Massachusetts, departure 22 Aug 1932, arrival 28 Aug 1932, manifest list, p.174, nos. 3, 4, Irene and Dorothy Bassett.
Note: It is believed both birth years are 4 years later than actual birth years.
12. "William H. Bassett," obituary, The Brooklyn Daily Eagle (Brooklyn, New York), 22 Jul 1934, p. 17, col. 3. Death: 21 July 1934, Cheshire, Connecticut.
Also, Headstone reads: William H Bassett / 1868 – 1934 / His Wife / Sarah H. Whiting / 1869 – 1956. Find A Grave, memorial 105408178, William Hastings Bassett (1868–1934), Rural Cemetery, New Bedford, Bristol County, Massachusetts with headstone photo.
13. Commonwealth of Massachusetts, Boston, Massachusetts, Death Index, 1941-1945, Dartmouth, Bristol County, Frederick C. Bassett, 1944, vol. 36, p. 202.
Also, Commonwealth of Massachusetts, Boston, Massachusetts, Death Index, 1951-1955, Fall River, Bristol County, Alvah H. Bassett, 1954, vol. 41, p. 337.
14. Manning's New Bedford and Fairhaven Directory for the year beginning January 1955, vol 74 (Springfield, Massachusetts : H. A Manning Company, 1955) p. 200, Charles M. and Irene Bassett.
– Bassett, Charles M (Irene) retired h166 Mill
Note: Written as if spouses; they were siblings.
Commonwealth of Massachusetts, Boston, Massachusetts, Death Index, 1951-1955, New Bedford, Bristol County, Charles Mendell Bassett, 1956, vol. 73, p. 24.
15. "Dorothy Bassett, Psychologist Dies at Age 67," obituary, The Daily Journal (Vineland, New Jersey), 28 Jan 1958, p. 2, col. 1.
Also, Commonwealth of Pennsylvania, Philadelphia, Philadelphia County, certificate of death, no. 7441, Dorothy M. Bassett, 28 Jan 1958. Residence: North Main Road, Vineland, Cumberland County, New Jersey. Age: 65. Birth: 14 Sept 1892, New Bedford, Massachusetts. Parents: William A. Bassett and Elmira D. Mayhew. Occupation: Psychologist, Public and Private Schools. Burial: Oak Grove Cemetery, New Bedford, Massachusetts, 31 Jan 1958.
Also, Find A Grave, memorial 233240660, Dorothy M. Bassett (1890–1958), Oak Grove Cemetery, New Bedford, Bristol County, Massachusetts; no headstone photo.
16. Commonwealth of Massachusetts, Boston, Massachusetts, Death Index, 1956-1960, New Bedford, Bristol County, Irene M. Bassett, 1959, vol. 75, p. 187.
Citing this page: Kimberli Faulkner Hull, author, "Irene Mayhew Bassett (1872–1959)," Cool Adventures (https://cooladventures.com/collection/irene-mayhew-bassett-1872-1959/ : posted 2021).