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Hoop's Top Picks for Families at the London Games Festival 2019
The team at Hoop (a free app that helps parents discover family activities) have picked out their top events for families at this year's London Games Festival.
Matt Eunson is Head of Engineering at Hoop and shared how his love of games set him on the path to a career in programming: 'As kids, we would try to mod the games we were playing and this how I first got interested in programming. It was exciting to be able to change my favourite games like Medal of Honor and this inspired me to learn how to create my own!
In fact, most of the people I've worked with say they first got into the industry because they loved games. So I'm excited to see that programming is now much more available to a younger audience, with languages like Scratch developed especially for children. Games can help teach children important skills such as logical thinking and problem-solving, which are key to a career in Engineering. The London Games Festival is a brilliant chance to celebrate games culture and help kids discover the possibilities of a career in coding.'
At Hoop, we're always working hard to support organisations that help kids discover their passions and learn new skills with fun, hands-on activities. That's why we're delighted to share our top picks for families at this year's London Games Festival:
Cosplay Parade and Family Day
Saturday 6th April | Guildhall Yard
A chance to make your own costume and accessories, before hundreds of video game characters take to the streets of London. There will also be stacks of free games exhibits and crafts suitable perfect for the whole family.
MythMash
Saturday 6th – Sunday 14th April | Now Play This Festival at Somerset House
MythMash provides a non-competitive space for collaborative, fantasy storytelling and worldbuilding. The game aims to encourage positive change through interactive experiences and to show others that they too have the power to create new worlds.
Trafalgar Square Games Festival
Saturday 13th April | Trafalgar Square
Trafalgar Square will be buzzing with exciting free activities, including a chance to play games competitively against the pro gamers, fun photo opportunities, life-sized retro games from the Actual Reality Arcade and much more.
Hoop is a free app that helps parents discover what's on for kids and is trusted by over a million families. Listing over 80,000 different family activities each week from creative coding and game design to local baby groups, dance classes, sports clubs and more.


For those that follow my blog closely you probably remember my epic roadtrip from Irvine, CA to Baltimore, MD. On my roadtrip I saw a lot of really cool places, people, and things. One of the highlights of the roadtrip was my visit to the Rocky Mountain National Park in Estes Park, Colorado. A truly beautiful place!
Even though I took a lot of pictures at the Rocky Mountain National Park, some of which you can see here, I finally uploaded videos I took during my visit last summer!
All the videos were taken driving up Trail Ridge Road and as I took in all the sights, sounds, and views of the stunning park. Getting higher gave you a better view and experience of the nature and surroundings. Of course driving up the narrow roads with 300 foot drops off the side can be a little nerve-racking. It was fun as well though! I highly recommend you go visit if you ever get a chance.
Took me long enough and hopefully I will get around to uploading more pictures and videos I that have languished on my laptop for a long time. Watch the videos below at least and let me know what you think!


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Int J Med Sci 2014; 11(8):765-770. doi:10.7150/ijms.8938
MicroRNA-365 Inhibits Vascular Smooth Muscle Cell Proliferation through Targeting Cyclin D1
Peng Zhang1,2, Cuiying Zheng3, Hui Ye1,2, Yan Teng2, Bin Zheng3, Xiao Yang2, Jishuai Zhang2
1. Model Organism Division, E-institutes of Shanghai Universities, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200025, China;
2. State Key Laboratory of Proteomics, Genetic Laboratory of Development and Disease, Institute of Biotechnology, Beijing, 100071, China;
3. Department of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, 050017, China.
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Zhang P, Zheng C, Ye H, Teng Y, Zheng B, Yang X, Zhang J. MicroRNA-365 Inhibits Vascular Smooth Muscle Cell Proliferation through Targeting Cyclin D1. Int J Med Sci 2014; 11(8):765-770. doi:10.7150/ijms.8938. Available from http://www.medsci.org/v11p0765.htm
MicroRNA-365 (miR-365) plays crucial roles in regulating cell proliferation, apoptosis and differentiation in various cell types. However, its function in vascular smooth muscle cells (VSMCs) is largely unknown. In our study, we found miR-365 was highly expressed in adult rat carotid arteries, but was significantly decreased in rat carotid arteries after balloon injury, a process involving neointima formation and VSMC proliferation. In vitro, the miR-365 significantly inhibited cell proliferation of isolated primary rat aortic VSMCs. Furthermore, we identified that cyclin D1 was a direct target of miR-365 in VSMCs. The miR-365 suppressed cyclin D1 expression on both mRNA and protein level. Luciferase reporter assay demonstrated that miR-365 inhibited cyclin D1 through targeting its 3'UTR. Importantly, cyclin D1 overexpression rescued the inhibitory effect of miR-365 on VSMCs proliferation. Taken together, by our studies, we identified a new MicroRNA, miR-365, involving in the pathological process of vascular injury, which inhibits VSMC proliferation through targeting cyclinD1.
Keywords: miR-365, vascular smooth muscle cells, proliferation, neointima formation, cyclin D1.
Vascular smooth muscle cells (VSMCs) are the main cell type of vascular wall and play an important role in vascular development and disease. VSMCs have high rate of proliferation and migration during early embryonic stages of vasculogenesis, while in adult blood vessels VSMCs become quiescent and proliferate at a very low rate [1]. Unlike skeletal and cardiac muscle cells which are terminally differentiated cells, VSMCs retain remarkable plasticity and can undergo phenotypic switching. In various forms of vascular diseases such as atherosclerosis, restenosis, vein bypass graft failure, and transplant vasculopathy, VSMCs switch from contracting phenotype to secreting phenotype, proliferate, and migrate into intima, contributing to neointima formation [2].
MicroRNAs (miRNAs) are endogenous, single-stranded RNA of 22 nucleotides and constitute a novel class of gene regulators [3, 4]. Increasing evidence indicates that MicroRNAs play critical roles in various VSMC functions and vascular diseases [5, 6]. In particular, miR-1, miR-31, miR-133, miR-143/145, miR-146a and miR-638 are involved in neointima formation after vascular injury by regulating VSMC proliferation, migration and phenotypic switching [7-13]. MicroRNA-365 (miR-365) is reported to regulate cell proliferation, apoptosis or differentiation in colon cancer cell lines [14], lung cancer cell lines [15], HaCaT cells [16], chondrocytes [17] and endothelial cells [18]. Our previous work showed that miR-365 inhibited gastric cancer cells proliferation, and participated in gastric cancer formation [19]. However, the function of miR-365 in VSMCs and neointima formation is still unknown.
In the present work, we demonstrated that miR-365 expression was significantly decreased in neointima formation model of rat carotid arteries in vivo, and miR-365 inhibited cell proliferation through targeting cyclin D1 in primary rat VSMCs in vitro. Our findings suggest that miR-365 probably is a novel important modulator for neointima formation.
Cell culture and transfection
Primary vascular smooth muscle cells were isolated from the thoracic aortas of male Sprague-Dawley rats as previously described [9]. The VSMCs were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 10% fetal bovine serum (FBS, SV30087.02, Hyclone) with 1% penicillin-streptomycin, and maintained at 37°C in a humidified atmosphere containing 5% CO2. The cells used in the experiments were from passage 3 to 5. Cells were individually plated on tissue culture plates or dishes 24 h before transfection. Transient transfection of miRNA mimics and control oligonucleotides (Genepharm) at a final concentration of 50 nM was accomplished with polyplus reagent (jetPRIME®). The cyclin D1 expression vector was constructed by inserting human cyclin D1 cDNA into the pCMV-HA vector (pCMV-HA-cyclinD1).
Proliferation assays
Cell count: 5×104 cells were seeded into 24-well plate in triplicate, and then transfected with miRNAs as described above [19]. After 6h transfection, the medium was changed into serum-free DMEM/F12 for starvation. 24 hours later, normal serum medium (10% FBS) was added (0h), and the cell number was counted at 24h, 48h, or 72 h under microscope using a hemocytometer.
BrdU incorporation assay: BrdU incorporation assay was performed and analyzed as described previously [10]. Briefly, cells grown on glass coverslips were labeled with 10 μM BrdU every eight hours. 24 hours later, the cells were fixed in 4% paraformaldehyde (PFA) for 30 minutes, and permeabilized in 0.5% Triton X-100 in PBS (pH 7.4) for 10 minutes. The fixed cells were incubated with 4N hydrochloric acid for 30 minutes at 37°, and were pre-blocked with goat serum for 30 minutes, and then incubated with rat anti-BrdU antibody (ab6326; 1:100; abcam) overnight at 4ºC. The sections were washed in PBS and incubated with goat anti-rat IgG/FITC for 1h at room temperature (ZF-0315; 1:200; ZSGB-Bio, Beijing, China). The nuclei were labeled with 4', 6-diamidino-2-phenylindole (DAPI). The BrdU incorporation rate was expressed as the percentage of BrdU-positive cells to total cell number per field.
Luciferase activity assay
The luciferase reporter plasmid containing human cyclin D1 3′-UTR sequence or mutant putative miR-365 seed sequence was generated as previously described [19]. The reporter plasmids, together with miR-365 or miRNA negative control, were transfected into cells using polyplus reagent (jetPRIME®), and luciferase activity was measured with the Luciferase assay kit (Promega).
Cells were homogenized in lysis buffer supplemented with protease inhibitor cocktail (Roche). Proteins (25ug) were electrophoresed on SDS-PAGE and transferred onto PVDF membranes. Immunoblotting was done using rabbit anti-cyclin D1 (2261-S; 1:1000; epitomics) and mouse anti-GAPDH (sc-25778; 1:3000; ZSGB-Bio) antibodies.
Determination of RNA levels by Real-time PCR
miRNAs were extracted from rat adult aortic VSMCs, aorta, carotid artery, heart, liver, spleen, lung, kidney, stomach and intestine by Trizol. The expression levels of mature miR-365 and U6 were measured using the Applied Biosystems TaqMan MicroRNA Assays system (7500 Fast; Applied Biosystems®). U6 expression was used to normalize the expression of miR-365. The 2-ΔCt method was used to analyze Real-time PCR data.
Balloon injury model
Carotid artery balloon injury was induced in male Sprague-Dawley rats (300 g) as previously described [9]. In brief, animals were anesthetized with urethane (600 mg/kg) intraperitoneally. The left common carotid artery was exposed through a midline cervical incision. The catheter was advanced from just under the proximal edge of the omohyoid muscle to the carotid bifurcation three times with a 2F (60 cm) Fogarty catheter (Baxter, McGaw Park, IL). To attain a constant degree of vessel wall injury for each of the animals, we kept the diameter of the balloon and the resistance during withdrawal constant for each of the animals. All procedures were performed by a single operator. Five rats were used in each group. All animal experiments were approved by the Animal Experiment Committee of the Institute of Biotechnology.
Histological analysis and immunohistochemistry
At 14 days after surgery, left carotid arteries were dissected and fixed with 4% PFA overnight. The arteries were subsequently paraffin-embedded, and serial 5-μm sections were cut. For morphometric analysis, sections were stained with hematoxylin and eosin (H&E) after deparaffinisation and rehydration. Immunohistochemistry staining of cyclin D1 was done using rabbit anti-cyclin D1 (2261-S; 1:200; epitomics) and goat anti-rabbit IHC kit (SP-9001; 1:200; ZSGB-Bio)
All values were expressed as mean ± SD. Statistical analysis was performed by 2-tailed Student's t test between two groups. The differences among three groups were determined using one-way analysis of variance (ANOVA) followed by Bonferroni's post-hoc tests. Results were considered statistically significant at P < 0.05.
miR-365 is expressed in rat carotid arteries, and is significantly decreased after balloon injury
When we detected miR-365 expression pattern in various tissues of rat by real-time PCR, we observed that, except stomach, miR-365 was significantly more expressed in aorta and carotid mainly composed of vascular smooth muscle cells, compared to other tissues (heat, liver, lung etc) (Fig. 1A). To evaluate whether miR-365 was involved in neointima formation, we subjected rats to carotid balloon injury, a well-established in vivo experimental model to study neointima formation [20]. At 14 days after surgery, the injured carotid shows obviously narrow vascular cavity, with thicker intima and media compared to no injury control (Fig. 1B). Real-time PCR analysis revealed that miR-365 expression was significantly down-regulated in the injured carotid arteries compared to control group (Fig. 1C, n = 5, P < 0.01). These results suggested that miR-365 may play a role in neointima formation.
miR-365 inhibits VSMC proliferation
The increased VSMC proliferation is contributed to the pathogenesis of neointima formation [21]. In our study, we evaluated the function of miR-365 in VSMC proliferation. The miR-365 was over-expressed using chemically synthesized mimic in primary rat aortic vascular smooth muscle cells. The Real-time PCR results showed that miR-365 expression was significantly increased by 51.3 folds after transfection (Fig. 2A). Next, we counted VSMCs cell number at different time points after miR-365 mimic transfection. At 48 hours post-transfection, the cell number was decreased by 40% in the miR-365-mimic-transfected cells compared with negative control (NC) cells (P < 0.01), and this decrease was pronounced by 60% at 72 hours (P < 0.01) (Fig. 2B). Furthermore, we detected whether the inhibitory effect of miR-365 on VSMC proliferation was due to cell division through BrdU incorporation. We observed that the percentage of BrdU-positive cells was significantly decreased by 58% (P < 0.01) (Fig. 2C). These results showed that miR-365 significantly inhibits VSMC proliferation.
Cyclin D1 is a direct target of miR-365
To explore the mechanism by which miR-365 inhibited VSMC proliferation, we attempted to identify miR-365 targets in VSMCs. Our previous results showed that cyclin D1 was a direct target of miR-365 in gastric cancer cells [19]. Herein, we firstly evaluated whether miR-365 could suppress cyclin D1 expression in primary rat VSMCs. The mRNA level of cyclin D1 was significantly decreased upon miR-365 transfection (Fig. 3A). Accordingly, the protein level of cyclin D1 was also dramatically reduced after miR-365 overexpression (Fig. 3B). Moreover, we observed that cyclin D1 expression was obviously increased in the injured carotid arteries compared with uninjured group (Fig. 3C, n = 5). These findings showed that the expression of miR-365 is inversely related to the cyclin D1 expression. To further confirm that cyclin D1 was a direct target of miR-365 in VSMCs, we performed the luciferase reporter assay. The results showed that miR-365 transfection significantly inhibited the luciferase activity of wild type cyclin D1 3′ UTR, but not the mutant cyclinD1 3′ UTR with the mutant biding site of miR-365 (Fig. 3D). These results demonstrated that cyclin D1 is a direct target of miR-365 in vascular smooth muscle cells.
Overexpression of cyclin D1 can rescue miR-365's effect on VSMCs proliferation
To evaluate whether decreased cyclin D1 mediate the inhibitory effect of miR-365 on VSMC proliferation, we transfected cyclin D1 plasmid without 3′ UTR in primary VSMCs. The western blot results showed that the expression of cyclin D1 was increased after transfection (Fig. 4A). When VSMCs were simultaneously transfected with cyclin D1 plasmid and miR-365 mimic, the results of cell count demonstrated that cyclin D1 overexpression rescued miR-365 induced growth inhibition (Fig. 4B, P < 0.01). In addition, BrdU incorporation results confirmed that cyclin D1 overexpression blocked the inhibitory effect of miR-365 on VSMC proliferation (Fig. 4C, P < 0.05). Together, these results implied that miR-365 inhibits VSMC proliferation through decreasing cyclin D1.
miR-365 expression is significantly decreased in neointima formation of rat carotid arteries. (A) Quantitative real-time PCR analyses of miR-365 expression level in various tissues: heart, liver, spleen, lung, kidney, stomach, intestine, aorta and carotid. The expression level of heart was set as 1. (B) Micrographs of arterial cross-sections with H & E staining without injury (No Injury) or at 14 days after injury (Injury) (n=5). Scar bar: 100 μm (C) Control and injured arterial segments were harvested for extraction of MicroRNA, followed by measuring the expression of miR-365 using real-time PCR. The expression level of miR-365 was normalized to U6. **P < 0.01.
(Click on the image to enlarge.)
miR-365 inhibits vascular smooth muscle cell proliferation. (A) Quantitative real-time PCR analyses of miR-365 expression after transfection with NC or miR-365 mimic. (B) The effect of miR-365 overexpression on vascular smooth muscle cell proliferation in vitro as measured by cell counts at 0, 24, 48, 72 hours after transfection with NC or miR-365 mimic. *P < 0.05, **P < 0.01. (C) BrdU incorporation experiment (BrdU, green; DAPI, blue) used as an independent manner to detect VSMCs proliferation at 24 hours after transfection with NC or miR-365 mimic. **P < 0.01. Scar bar: 50 μm.
cyclin D1 is a direct target of miR-365 in vascular smooth muscle cells. (A) Quantitative real-time PCR analyses of cyclin D1 expression in VSMCs after transfection with NC or miR-365 mimic. **P < 0.01. (B) Left panel: representative western blot of cyclin D1 in VSMCs after transfection with NC or miR-365 mimic. Right panel: quantification of cyclin D1 via western blot. **P < 0.01. (C) Representative immunohistochemistry staining of cyclin D1 in carotid arteries with or without injury (n = 5). Scar bar: 50 μm. (D) Luciferase activities were measured in VSMCs cotransfected with the reporter constructs containing wild type (WT) or mutant (Mut) 3'UTR of cyclin D1, and NC or miR-365 mimic. **P < 0.01.
miR-365 inhibits VSMC proliferation by targeting cyclin D1. (A) Representative western blot of cyclin D1 expression in VSMCs after transfection with CMV-HA or CMV-HA-cyclin D1 overexpression constructs. (B) Cyclin D1 overexpression blocked the inhibitory effect of miR-365 on VSMC proliferation as measured by cell count at 0, 24, 48, 72 hours after transfection with NC plus CMV-HA, miR-365 plus CMV-HA or miR-365 plus CMV-HA-cyclin D1. *P < 0.05, **P < 0.01 for miR-365 plus CMV-HA-cyclin D1 vs. miR-365 plus CMV-HA (one way ANOVA and post-hoc tests). (C) Left panel: representative immunofluorescent staining of BrdU (green) and DAPI (blue) showing the VSMC proliferation after transfection with NC plus CMV-HA, miR-365 plus CMV-HA or miR-365 plus CMV-HA-cyclin D1. Scar bar: 50 μm. Right panel: quantification of BrdU incorporation in VSMCs transfected with NC plus CMV-HA, miR-365 plus CMV-HA or miR-365 plus CMV-HA-cyclin D1. **P < 0.01 for miR-365 plus CMV-HA vs. NC plus CMV-HA, #P < 0.05 for miR-365 plus CMV-HA-cyclin D1 vs. miR-365 plus CMV-HA (one way ANOVA and post-hoc tests).
In the present study, we found that miR-365 had a high expression in vascular smooth muscle tissues such as carotid, and was dramatically decreased in carotid arteries after balloon injury. Next, we demonstrated that miR-365 significantly inhibited cell proliferation in primary rat VSMCs. Moreover, we revealed that cyclin D1 was a direct target of miR-365, and cyclin D1 overexpression blocked the inhibitory effect of miR-365 on VSMC proliferation.
For the first time, we identified the function of miR-365 in VSMCs. It has been reported that miR-365 regulates cell proliferation, apoptosis and differentiation in various cell types [14-17]. However, its function in VSMCs is still unknown. In this study, we demonstrated that miR-365 overexpression significantly inhibited VSMC proliferation. Previous works show that miR-365 inhibits cell proliferation in lung cancer [15], gastric cancer [19] while it promotes cell proliferation in cutaneous squamous cell carcinoma [16] and chondrocytes [17], suggesting its function in regulation of cell proliferation is dependent on cell type. Our results showed that miR-365 had an inhibitory effect on cell proliferation in VSMCs.
The VSMC proliferation plays an important role in carotid neointima formation after injury [2]. In response to injury, media VSMCs are activated and migrate to the intima, where they proliferate and subsequently produce abundant extracellular matrix (ECM) to form the neointima [21]. More recently, increasing evidences showed that MicroRNAs participate in VSMC proliferation and neointima formation. The miR-146a, miR-31, miR-221 and miR-222 promoted VSMC proliferation, and inhibition of miR-146, miR-221 and miR-222 by adenovirus significantly decreased neointima formation in response to balloon injury [8, 9, 22], while miR-133 and miR-1 inhibited VSMC proliferation, and Adeno-miR-133 significantly reduced neointimal hyperplasia after balloon injury [10, 23]. Our work demonstrated that miR-365 significantly inhibited VSMC proliferation in vitro. In addition, miR-365 expression was significantly decreased in carotid arteries in response to injury in vivo, suggesting miR-365 may be a novel important MicroRNA involved in neointima formation.
Our further study identified cyclin D1 as a direct target of miR-365 in primary VSMCs. To evaluate whether the decreased cyclinD1 mediates the inhibitory effect of miR-365 on VSMCs proliferation, we over-expressed cyclinD1 in primary rat aortic smooth muscle cells, and found that cyclin D1 overexpression partially rescue the inhibition of VSMCs proliferation after miR-365 transfection. The MicroRNA is known to have hundreds of targets to mediate its function [3]. In this study, although we provide evidence to show cyclin D1 is the target of miR-365 in VSMCs, we could not rule out that the other targets mediate the inhibitory effect of miR-365 on VSMC proliferation.
In summary, we demonstrate that miR-365 inhibits VSMC proliferation through targeting cyclin D1. The down-regulation of miR-365 in injured carotid arteries suggests that miR-365 may participate in the neointima formation, and can be used as a potential therapeutic target in preventing vascular proliferation diseases.
VSMCs: vascular smooth muscle cells; MicroRNAs: miRNAs.
This study was funded by the National Natural Science Foundation of China (NO. 81270165), and State Key Laboratory of Proteomics Grant (No. SKLP-K201102).
The authors have declared that no competing interest exists.
1. Owens GK, Kumar MS, Wamhoff BR. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev. 2004;84:767-801
2. Gibbons GH, Dzau VJ. The emerging concept of vascular remodeling. N Engl J Med. 1994;330:1431-8
3. Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP. et al. The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science. 2005;310:1817-21
4. Ambros V. MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell. 2003;113:673-6
5. McDonald RA, Hata A, MacLean MR, Morrell NW, Baker AH. MicroRNA and vascular remodelling in acute vascular injury and pulmonary vascular remodelling. Cardiovasc Res. 2011;93:594-604
6. Urbich C, Kuehbacher A, Dimmeler S. Role of microRNAs in vascular diseases, inflammation, and angiogenesis. Cardiovasc Res. 2008;79:581-8
7. Xie C, Huang H, Sun X, Guo Y, Hamblin M, Ritchie RP. et al. MicroRNA-1 regulates smooth muscle cell differentiation by repressing Kruppel-like factor 4. Stem Cells Dev. 2010;20:205-10
8. Liu X, Cheng Y, Chen X, Yang J, Xu L, Zhang C. MicroRNA-31 regulated by the extracellular regulated kinase is involved in vascular smooth muscle cell growth via large tumor suppressor homolog 2. J Biol Chem. 2011;286:42371-80
9. Sun SG, Zheng B, Han M, Fang XM, Li HX, Miao SB. et al. miR-146a and Kruppel-like factor 4 form a feedback loop to participate in vascular smooth muscle cell proliferation. EMBO Rep. 2010;12:56-62
10. Torella D, Iaconetti C, Catalucci D, Ellison GM, Leone A, Waring CD. et al. MicroRNA-133 controls vascular smooth muscle cell phenotypic switch in vitro and vascular remodeling in vivo. Circ Res. 2011;109:880-93
11. Cheng Y, Liu X, Yang J, Lin Y, Xu DZ, Lu Q. et al. MicroRNA-145, a novel smooth muscle cell phenotypic marker and modulator, controls vascular neointimal lesion formation. Circ Res. 2009;105:158-66
12. Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G. et al. down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic protein 4. J Biol Chem. 2011;286:28097-110
13. Pannu H, Fadulu VT, Chang J, Lafont A, Hasham SN, Sparks E. et al. Mutations in transforming growth factor-beta receptor type II cause familial thoracic aortic aneurysms and dissections. Circulation. 2005;112:513-20
14. Nie J, Liu L, Zheng W, Chen L, Wu X, Xu Y. et al. microRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2. Carcinogenesis. 2012;33:220-5
15. Kang SM, Lee HJ, Cho JY. MicroRNA-365 regulates NKX2-1, a key mediator of lung cancer. Cancer Lett. 2013;335:487-94
16. Zhou M, Liu W, Ma S, Cao H, Peng X, Guo L. et al. A novel onco-miR-365 induces cutaneous squamous cell carcinoma. Carcinogenesis. 2013;34:1653-9
17. Guan YJ, Yang X, Wei L, Chen Q. MiR-365: a mechanosensitive microRNA stimulates chondrocyte differentiation through targeting histone deacetylase 4. FASEB J. 2011;25:4457-66
18. Qin B, Xiao B, Liang D, Xia J, Li Y, Yang H. MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression. Biochem Biophys Res Commun. 2011;410:127-33
19. Guo SL, Ye H, Teng Y, Wang YL, Yang G, Li XB. et al. Akt-p53-miR-365-cyclin D1/cdc25A axis contributes to gastric tumorigenesis induced by PTEN deficiency. Nat Commun. 2013;4:2544
20. Kumar A, Lindner V. Remodeling with neointima formation in the mouse carotid artery after cessation of blood flow. Arterioscler Thromb Vasc Biol. 1997;17:2238-44
21. ER OB, Ma X, Simard T, Pourdjabbar A, Hibbert B. Pathogenesis of neointima formation following vascular injury. Cardiovasc Hematol Disord Drug Targets. 2011;11:30-9
22. Liu X, Cheng Y, Zhang S, Lin Y, Yang J, Zhang C. A necessary role of miR-221 and miR-222 in vascular smooth muscle cell proliferation and neointimal hyperplasia. Circ Res. 2009;104:476-87
23. Chen J, Yin H, Jiang Y, Radhakrishnan SK, Huang ZP, Li J. et al. Induction of microRNA-1 by myocardin in smooth muscle cells inhibits cell proliferation. Arterioscler Thromb Vasc Biol. 2010;31:368-75
Author contact
Corresponding author: E-mail address: kdliuedu.cn
Received 2014-2-26
Accepted 2014-3-26
Published 2014-5-21

redpajama_set_name: RedPajamaCommonCrawl


text:
Q: How to add existing Template to Visual Studio? Particularly for VS.NET 2013, how do you add a template?  I've created a custom template. VS.NET adds it to \Libraries\Documents\Visual Studio 2013\Templates\ItemTemplates.  I'd like to know import that template into VS.NET on a different machine.
When I rename the zip template in the above location, that name doesn't show up in the add new item dialogue box in VS.NET.  Are there any steps for adding a template?

A: Maybe you can add the template to [Visual Studio installation path]\Common7\IDE\ItemTemplates\  of the other machine.
And then run the command devenv /installvstemplates in your Developer Command Prompt
Source:
http://msdn.microsoft.com/en-us/library/ms241279.aspx

redpajama_set_name: RedPajamaStackExchange


Brown, in his book Plan B 4.O: mobilizing to save civilization shows that we are facing some very large, what Heifitz in his article Leading with an open heart calls adaptive challenges. Adaptive challengesoccur when society is faced with the necessity to completely change how its views, and operates within the world. Brown clearly explains that the way we have "always done things" isn't necessarily most beneficial to us, or our planet by outlining current problems such as decreasing food, water, economic and social security. We cannot sustain our current pattern of growth, along with our current use of resources. Brown, in no uncertain terms, lays down the facts and he has very specific ideas of what will help us face these challenges. The problem is, who is going to lead the world in changing ingrained behavior? Are we going to be able to face these adaptive challenges successfully? Heifitz says, "To meet adaptive challenges, people have to go through a period of painful adjustment. Leading people to make these changes is risky,vbecause you are asking them to absorb various forms of loss—asking them to out and out give up something in the interests of something to be maintained, to be conserved, or to be gained" (Heifitz, 2002, p 29). But it is possible.
In a conversation, Heifitz would probably tell Brown that in order to bring about the changes he thinks are necessary to remedy or even reverse some of the damage we have done to the planet, we need leaders that are grounded and self aware. Leaders often become leaders because they take the responsibility off of others' shoulders. (Heifitz, 2002, p. 31) In order to deal with these adaptive challenges we need compassionate leaders who are good at making the responsibility broad based. Heifitz would also emphasize the need to embrace conflict. Most people, including leaders tend to avoid conflict making often making mild disagreement into violent disagreement Heifitz says, "Successful leaders manage conflict; they don't shy away from it or suppress it but see it as an engine of creativity and innovation." The conflict arising from debates over how to deal with climate change, water shortages, and food security, if handled correctly could be fuel for incredible creative solutions.
Brown would most likely speak to Heifitz with a sense of urgency, and an emphasis on the technology needed to create a more efficient and peaceful world. I think he would agree with Heifitz's ideas on leadership in general, but might be a bit frustrated with Heifitz views on conflict. Brown has a very clear purpose, and believes that we need to take drastic and specific actions in order to restore balance. This may be true, but but the nearly 8 billion people on this planet may not all agree. The challenge in facing such adaptive changes is that there will be inevitably some conflict before creative solutions can be put in place. Brown, however, would also tell Heifitz that open hearted leaders are already shaping the future, and are the key to its success. He says "We face an extraordinary challenge, but here is much to be upbeat about" and goes on to give examples of countries taking the lead on restoring the planet's health (Brown, 2009, p. 25). We have all the tools in technology andleadership to implement Plan B, now we have to use them.
This entry was posted on Tuesday, September 20th, 2011 at 1:36 pm and is filed under Uncategorized.	You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.
Brown is masterful at pushing the urgency of our global environmental challenges. I think it's an excellent point that while adaptive challenges take time, we don't have a lot of time right now for our planet's air and water resources.


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text:
InsightUnlocked
Sabine Doolin
QWB Lab
Case study: Visitor Experience Plan
A 5-year Visitor Experience Plan for MOTAT
Bringing everything relating to MOTAT's visitor experience together in a strategic roadmap for the whole organisation.
A collaboration of InsightUnlocked and Sally Manuireva Consulting.
Client: MOTAT, Museum of Transport and Technology, Auckland
Spread across 40 acres and two museum sites plus its collections stores, MOTAT, the Museum of Transport and Technology in Auckland, is the country's largest transport and technology museum of its kind and offers experiences for visitors of all ages. MOTAT's vision is to use past, present and future Kiwi technology to educate and inspire the innovators of tomorrow. Since this vision was articulated in 2014 MOTAT has been on a journey to transform from a "museum of things" to a "lightbulb institution" that is audience focused, vision based and has a transformative impact on its community.
Challenge: Create a whole-organisation approach to the visitor experience
MOTAT had undertaken a range of useful initiatives to advance its audience development and visitor experience. However, it felt that these could be more closely aligned and have a stronger shared focus on future plans. To accelerate achieving its strategic objectives, MOTAT was seeking a clearer direction and more unified approach to the visitor experience across all three of the museum's hubs (experience, collection and business services) – a strategic roadmap for the whole organisation.
Approach: Strategic and co-created with the organisation
In a first in-depth working session with Steven Fox, General Manager Museum Experience, we established MOTAT's challenges and needs. From there, the idea of a 5-Year Visitor Experience Plan took shape – a plan that closely linked to the museum's vision and brought everything related to the visitor experience together in one place.
A key focus was to gain broad buy-in for the plan. Everyone in the museum touches on the experience of the visitor in some way so it was important to create a plan for the whole museum with strong links to the collection.
After an analysis of the museum's strategic documents and its previous visitor related initiatives, as well as some site visits, we developed a question framework to structure the Plan. We then took a co-creation approach involving staff from across the organisation to get broad input early on and ran a range of focused workshops with various teams.
Visitor Experience Plan question framework
From this we developed a Visitor Experience Plan that describes the aspiration – MOTAT's visitor experience at its best, articulates the audience strategy, maps out themes and priorities in a roadmap for the next 5 years as well as provides a decision making tool supporting staff in applying the Plan in practical decision making. Working with Steven and his senior team throughout, we checked back with the teams for their feedback before finalising it. To accompany the final detailed Plan, we developed a one-page visualisation that can be used as a hand-out for staff, a visual on the intranet and a wall poster as easy, at-a-glance reference and for broad presence of the Plan.
Implementation: Bringing the Plan to life
A strategy is only as good as its implementation, so we developed an Activation Programme to empower staff to take ownership of the visitor experience and to actively use the 5-Year Plan. The Activation Programme now unfolds in several phases from communicating the Plan, to starting its application until it reaches the stage of "embedded practice." It includes a staff feedback loop and a phase to refresh the Visitor Experience Plan for the following year, as required.
Outcome: Jointly owned across the organisation
The first step of the Activation was the launch of the Plan. For this, MOTAT closed a little earlier one afternoon to allow all staff to attend a presentation by Steven, supported by the Chief Executive and the leaders of the Collections and the Business Services departments, demonstrating their joint support for the visitor experience.
Judging from the questions and reactions from staff, the Visitor Experience Plan is off to a great start.
Steven Fox, General Manager Museum Experience, with Sally and Sabine at the staff launch of the Visitor Experience Plan in MOTAT's Aviation Hall
download the CASE STUDY (PDF)
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Conveniently located, with road frontage on Burleson Manor Rd, this property could service as a site for light industrial, commercial, residential or a mix of uses. OWNER FINANCE is available and the property is unrestricted. Manville water is on the property. With the future extension of Braker Lane, the property will be in close proximity to the future intersection of Burleson Manor and Braker Lane. Call for details.


The chipmaker also shared more information about its product roadmap, including next-generation Xeon processors.
AI for business: Why artificial intelligence and machine learning will be revolutionary There's lots of hype around AI and Centrica CIO Mike Young says IT leaders must ground their efforts in business reality.
Intel collected more than $1 billion in revenue in 2017 from Xeon processors used for artificial intelligence in the data center, the company said Wednesday.
Navin Shenoy, EVP and GM of the Data Center Group at Intel, announced the milestone during Intel's Data Centric Innovation Summit, where he also shared more information about Intel's product roadmap. All told, Intel's data-centric businesses amount to a market opportunity that should be worth $200 billion in 2022, Shenoy said. Previously, Intel estimated its TAM (total addressable market) at $160 billion.
The technology industry, Shenoy said, needs to view data holistically, "including how we move data faster, store more of it and process everything from the cloud to the edge."
The remarks were in keeping with the approach to AI that Gadi Singer, VP of Intel's Artificial Intelligence Products Group, laid out to ZDNet. Intel, he said, is building a successful AI business by investing in Xeon as "the bedrock of AI," building a diverse chip portfolio, and investing in systems integration.
As far as systems integration goes, Intel announced Wednesday that it's expanding its connectivity portfolio with a new SmartNIC product line, code-named Cascade Glacier. Intel also announced the first shipments of its Optane DC persistent memory modules, starting with a symbolic hand-delivery to Google. On stage at Wednesday's summit, Shenoy gave the first unit to Bart Sano, Google's vice president of Platforms.
Cascade Lake, a future Xeon Scalable processor based on 14nm that will introduce Optane DC persistent memory and DL Boost with Vector Neural Network Instruction (VNNI).
Cooper Lake, a future Xeon Scalable processor that is based on 14nm technology. It will introduce a new generation platform with significant performance improvements, new I/O features, new DL Boost capabilities (Bfloat16) that improve AI/deep learning training performance, and additional Optane DC persistent memory innovations.
Ice Lake, a future Xeon Scalable processor based on 10nm technology that shares a common platform with Cooper Lake.


text:
Sad 90's alt rock video from MTV - trashed room ends up on fire [closed]
This question is an identification question which is considered off-topic. While they were temporarily allowed on the site for a point of time, they are fully off-topic again.
Want to improve this question? Update the question so it's on-topic for Music Fans Stack Exchange.
Closed 10 days ago.
There was an alt rock video I saw on MTV in the 90's. All I really remember is a guy sitting or crouched in the corner of a dark trashed room and the walls end up on fire, and I remember it was sad.
I know it's a long shot but any help would be appreciated. This video has haunted me for years but I can't quite remember it (much like Solitude Auternus's song "Midnight Dreams" did for 14 years before someone helped me figure it out).
identify-this-song identify-this-video
Bebs
charliecharlie
It could be REM losing my religion or a bush song
Note: This answer is based on deleted answers by Tina Knights and user1962
Your description seems to be a good match for 90's era hard rock band Staind's 2001 hit, "It's Been Awhile".
To quote the Wikipedia article on the song
The music video begins with Aaron Lewis going through some old photographs, one of which happens to be the picture of his wife. The video then switches between him writing a letter to his wife and the band performing casually in a room full of candles. There are brief shots of Aaron alone in the streets and looking at himself in the mirror while having second thoughts. Throughout the video, Aaron is seen continuously smoking cigarettes. At the end of the video, one of those cigarettes falls onto the floor and burns down his apartment.
Chris Sunami supports Monica
Why have these answers been deleted if they might be correct?
– Bebs
@Bebs - Please see this meta discussion: musicfans.meta.stackexchange.com/questions/283/… . Also this one as well: musicfans.meta.stackexchange.com/questions/285/…
– Chris Sunami supports Monica
Chris, I agree with you, but once it was agreed the answer was deleted by mistake, shouldn't we undelete the answer. To me there is no reason to delete an answer because it is too short, on can downvote or at least convert it to a comment, but deleted ?! I still don't see why. But I suppose, in Music Fans SE, Bosses have to show their power once in a while.
@Bebs These were all deleted over a year ago, prior to that discussion --I think the mods have had a lighter touch since then. I also think it's an overreach to hint that this was an abuse of power, the answers as they stood were pretty poor. At any rate, I pointed out those meta threads not to indicate this is a settled issue, but to suggest that there would be the best place to continue the discussion about it.
Could this be Ricky Martin's "Private Emotion" (1999)? https://www.youtube.com/watch?v=N-m9fn1yeEI . The video is a time-reversed video that starts with a trashed hotel room and runs backwards to when the room is on fire.
Chris Sunami supports MonicaChris Sunami supports Monica
No, I checked it out just to make sure but no it wasn't that song or video. It would have been some sort of rock,grunge,or metal. It was really the only music I was into back then. Thank you though, I do appreciate it.
– charlie
My first thought when you said this was a little bit newer than the 90's (2007)...check out One Republic's "Stop and Stare". This video/song stuck with me-even though I don't have any One Republic records-because it was a strong enough video I remembered it. It has fire in the end, and it is a slower/sadder kind of song. I'll be curious to know.
t1nr2yt1nr2y
Not the answer you're looking for? Browse other questions tagged identify-this-song identify-this-video or ask your own question.
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Ghana's wing back, Kwadwo Asamoah, has been named in South Africa's version of Africa's Finest XI team of 2018.
The 30-year-old Inter Milan star was the only Ghanaian to have made it to the finest Africa team which was voted by 23 Bafana Bafana stars.
Thomas Partey who made it to the Continent's finest XI squad did not find a place in the team of South Africa's version as he was replaced by Nigeria's Victor Moses in midfield.
Unlike Kwadwo Asamoah who was preferred over Mehdi Benatia in the official CAF list, Kalidou Koulibaly, Eric Bailly and Sergei Aurier were all maintained.
They made five changes to the CAF list which included Mamelodi Sundowns shot-stopper, Denis Onyango, for Kaiser Chiefs goalie, Itumeleng Khune, while Strasbourg's Lebo Mothiba and Chiefs' Khama Billiat were preferred over CAF's Sadio Mane and Pierre-Emerick Aubameyang.
Riyad Mahrez and Naby Keita were their preferred choice for midfield with Egyptian and Liverpool star, Mohamed Salah also keeping his position for the striking option.
The list have Itumeleng Khune as the goalkeeper, Kalidou Koulibaly, Eric Bailly and Serge Aurier and Ghana wing-back Kwadwo Asamoah for the defense, the midfield role was handed over to Riyad Mahrez, Naby Keita and Victor Moses with the attackers going to Lebo Mothiba, Khama Billiat and Mohamed Salah.


The birth of the Duke and Duchess of Cambridge's baby, and heir to the throne, is a cause for great celebration. The Lord-Lieutenant, on behalf of the people of Kent, congratulates Their Royal Highnesses. All of us share their joy at the safe delivery of their son, and send our best wishes for their family's future happiness.


Reimer, C.W. 1966. Consideration of fifteen diatom taxa (Bacillariophyta) from the Savannah River, including seven described as new. Notulae Naturae 397: 1-15.
Phycology Section, Patrick Center for Environmental Research, Academy of Natural Sciences of Drexel University. Achnanthes lanceolata var. abbreviata (NADED 2114). https://diatom.ansp.org/taxaservice/ShowTaxon1.ashx?naded_id=2114. Accessed 24 Apr 2019. This web service reports all information about a given algae taxon available at the time of retrieval.


WASHINGTON (AP) — Philipp Grubauer starts rarely enough that he doesn't want to show his face when he loses. He can show it plenty until his next chance.
Grubauer stopped a season-high 38 shots to shut out the Los Angeles Kings as the Washington Capitals ended Peter Budaj's shutout streak in a 5-0 rout Sunday. Washington snapped Los Angeles' winning streak at five and Budaj's shutout streak at 147:21 in winning its third game in a row.
Despite being outshot 38-20, the Capitals were opportunistic in shelling Budaj, limiting quality chances and relying on Grubauer to make the routine saves.
"He doesn't get in there all the time, but when he does he's just been really good," said Brett Connolly, who scored his 10th goal of the season. "There was a couple times we turned the puck over and they had a good chance in front and he was there to bail us out."
Grubauer denied Marian Gaborik from close range in the second period but didn't have to make too many 10-bell saves for his third shutout of the season. Coach Barry Trotz liked the team defense in front of Grubauer, who compared the new NHL-mandated slimmer goalie pants to "European skinny jeans."
"The guys did an amazing job keeping (shots) to the outside today," Grubauer said. "I don't think we gave up too many Grade-A chances."
They left Lars Eller, Marcus Johansson and Justin Williams wide open on their goals, and Connolly was in all alone on a semi-breakaway to score his. T.J. Oshie's goal popped the cap off Budaj's water bottle, sending water flying and adding insult to injury.
Budaj, who leads the NHL with seven shutouts, was pulled after allowing four goals on 15 shots in two periods. He had stopped all 39 shots in his past two starts, but this loss was more on the Kings' defensive miscues than their goaltender.
"When you don't manage the puck well and give them opportunities to score, they're going to score," defenseman Jake Muzzin said. "You give them a little space and they capitalize on that. That's the danger, and we gave them too much space."
Coach Darryl Sutter said no one wants to get into an All-Star Game against the Capitals because the Kings are "not in that class." When the Capitals are getting this kind of goaltending and depth of scoring, few teams in the league are.
Washington leads the Metropolitan Division and Eastern Conference by seven points and is five up on the Minnesota Wild in the Presidents' Trophy race that also comes with home-ice advantage throughout the playoffs. Trotz said the team isn't thinking about the standings but is focused on each game.
Good thing for the Capitals that with Vezina Trophy winner Braden Holtby and Grubauer, they have a goalie they can turn to each game who can steal points if needed.
"Both goalies are tremendous," captain Alex Ovechkin said. "I think we have best duo in the league. When Holts have a break, Grubi stepping up and show he can be tremendous as well."
NOTES: Jeff Zatkoff stopped four of five shots in relief of Budaj, allowing Williams' 18th goal of the season. Connolly has 10 goals in 39 games after scoring nine in 71 games for the Boston Bruins last season. Oshie's goal was the pending unrestricted free agent's 20th of the season. The Kings put C Jordan Nolan on injured reserve with a lower-body injury suffered Wednesday. Ovechkin was honored before the game for recording his 1,000th point last month. NHL senior vice president Jim Gregory presented him with a Tiffany crystal, while Capitals owner Ted Leonsis gave Ovechkin a gold stick. During the ceremony, Leonsis held up a cutout of Ovechkin's head that was given to all fans.
Kings: Travel to Florida to face the Tampa Bay Lightning on Tuesday in their third stop on a four-game road trip.
Capitals: Look to make it four in a row when they host the Carolina Hurricanes on Tuesday, the teams' second meeting in just over two weeks.
——— Follow Hockey Writer Stephen Whyno on Twitter at http://www.twitter.com/SWhyno .


Stella is in here crate in the kitchen. Liam is watching Saturday morning cartoons. Dorothea and Madeline went to Aveda so Madeline could get her hair cut.
Suddenly, I am all wet. It may be a quite a humid day. Like the article in the New Yorker about the tourists in New Guinea, white, sweating; the locals, dry.
PT this morning, doing the best that I have in quite awhile. Felt neutral after tranction. Haven't had the numbness or tinkling in my tibia or fibia for awhile. Back to doing exercises.
At Bread and Chocolate after going to the Unitarian church with Dorothea and th e children and sitting through the beginning bit of the children's service. Usually it is seperate from the main service, But these must be a forth Sunday or something like that. After a story about sacred spaces from the woman who is the director of religious education, and who does a great job, the children were shuffled off to Sunday school classrooms. I ducked out. Significantly, this is the first time I have made a walk away from there since hurting my back.
Hungry. If I oly had a paper, 'twould be perfect. But then, without the distraction of a paper, all I have to do is write.
I am at Caribou on Grand Avenue. Caribou is a big corporation. So much for my morals.
Earth Day makes me think of Sister Michele, my fifth grade homeroom and science teacher, the first woman I knew with a mustache. I definitely did not like Sister Michele.
Well, I am not going to Nina's anymore. So that is a change in my life. A change which means that most of my blogging is up in the air as well. I have tried sending a message from the pager that I have for work. Don't get paged much on it if ever, so maybe I can use it for my own. Below is an undoctored pager email.
"Things have changed a lot" says Dorothea. They are a lot different then they were fifteen years ago.
Continue reading Our anniversary looms.
Things have gotten pretty quiet. Perhaps it is just that it is spring. I haven't had the focus to blog. Plus, I have stopped going to Nina's, which was my blogspot. So changes are afoot and I haven't decided yet how to deal with them.


text:
「Jaipur, Rajasthan」の検索結果を表示中
ジャイプル
1. アンベール城
"Amer Fort is a fort located in Amer, Rajasthan, India. Amer is a town with an area of 4 square kilometres (1.5 sq mi)[1] located 11 kilometres (6.8 mi) from Jaipur, the capital of Rajasthan. Located high on a hill, it is the principal tourist attraction in Jaipur.[2][3] The town of Amer was originally built by Meenas,[4] and later it was ruled by Raja Man Singh I. Amer Fort is known for its artistic style elements. With its large ramparts and series of gates and cobbled paths, the fort overlooks Maota Lake,which is the main source of water for the Amer Palace."
歴史上有名な所
2. Hawa Mahal
"Hawa Mahal was built in the year 1799 for the woman of the royal palace. In medieval India a 'pardah' system was followed where the royal woman followed seclusion. In order for royal woman to be connected to the outside would the Hawa Mahal was built. The queens and princesses could look at the various processions and events through the many windows of the Hawa Mahal."
3. Nahargarh Fort Jaipur.
"Nahargarh fort built in the year 1734 by Maharaja Sawai Jai Singh and was originally named as Sudarshangarh, but it became known as Nahargarh which means "abode of tigers". It stands on the edge of the Aravalli hills and you may see the whole Jaipur sight after taking an stand on the top of the fort hill boundary wall. It also represent the strong defense ring for the city."
4. アルバート・ホール博物館
"Albert Hall Museum is located in Rajasthan, India. It is the oldest museum of the state and functions as the state museum of Rajasthan. The building is situated in Ram Niwas garden outside the city wall opposite New gate and is a fine example of Indo-Saracenic architecture."
5. Jantar Mantar - Jaipur
"The Jantar Mantar is a collection of nineteen architectural astronomical instruments built by the Kachwaha Rajput king Sawai Jai Singh II, the founder of Jaipur, Rajasthan. The monument was completed in 1734. It features the world's largest stone sundial, and is a UNESCO World Heritage site. Wikipedia"
6. Jaigarh Fort
"Jaigarh Fort is situated on the promontory called the Cheel ka Teela (Hill of Eagles) of the Aravalli range; it overlooks the Amer Fort and the Maota Lake, near Amer in Jaipur, Rajasthan, India. The fort was built by Jai Singh II in 1726 to protect the Amer Fort and its palace complex and was named after him."
ティールーム
7. Tapri Central
"Great place for tea-lovers! This place offers a variety of teas and Indian snacks with beautiful rooftop ambiance!"
"Experience the rich architecture and culture of Jaipur. This place houses the entire collection of clothes, weapons, fabric etc of the Jaipur Royal family. "
9. House of People
"This is one of the most happening clubs of Jaipur. Give it a try if you have some spare time."
自主映画館
10. Raj Mandir Cinema
"Historic cinema hall which is now just not cinema hall but it's more of a tourist destination. Single screen cinema hall in this time of multiple screen era but still hard to get tickets. Beautiful piece of architecture. Feel like u entered a palace. Outside and inside it's just something you can't miss if you are in Jaipur. Must visit at least once. "
11. Jal Mahal
"Jal Mahal is a palace in the middle of the Man Sagar Lake in Jaipur city, the capital of the state of Rajasthan, India. The palace and the lake around it were renovated and enlarged in the 18th century by Maharaja Jai Singh II of Amber. "
12. City Palace
"A complex of courtyards, gardens and buildings, the impressive City Palace is right in the centre of the Old City. The outer wall was built by Jai Singh II, but within it the palace has been enlarged and adapted over the centuries. There are palace buildings from different eras, some dating from the early 20th century. It is a striking blend of Rajasthani and Mughal architecture. Our heritage home in Jaipur is located within 7 minutes' drive by tuk-tuk / taxi to the majestic City Palace. "
13. Blackout
14. Caffé Palladio Jaipur
"Beautiful terrace area to sit during the day. Italian and Turkish inspired food and great juices/shakes. "
15. Anokhi Café
"A cafe that offers healthy and tasty fresh food with an international flavour. (kindly note: The cafe DOES NOT offer Indian food, many other places in Jaipur offer great Indian food) As far as possible the cafe offers foods that are made from fresh seasonal organic produce grown locally at Anokhi farm. Their salads for example are made from home grown organic lettuce that is freshly picked every morning and served the same day, it is washed with bottled mineral water of course!"
16. Amer Palace and Fort
"Main attraction of Jaipur. Please check sheeshmahal and enquire about the sound and light show at this place."


Based on the weak and jagged main output ramp on the previous page and my earlier hypothesis that the main converter IC is not receiving sufficient power to operate normally, I would say almost certainly yes. The nice thing about knowing how the circuit works and having an oscilloscope is that I can hook it up and see how badly.
The first slide shows the bottom capacitor voltage, auxiliary output voltage and PWM controller supply voltage while the main outputs are off. Something looks very wrong here. I was expecting to find out what the nominal voltage on the auxiliary supply was, but its waveform peaks at -132V and does not appear to be holding any voltage at all, dropping to about -156V half-way between pulses. This is so different from what I was expecting to see that I had doubts about somehow soldering my probe wire to the wrong trace--the long, relatively fat, unmistakable one that links the auxiliary output to the PWM section. On the PWM's side, the fast edges from the auxiliary supply ripples are causing glitches to pass through. However, the output is otherwise holding steady at about 8V, which is consistent with the 3844B's under-voltage lock-out, so the PWM's bypass capacitor might still be good. Perhaps one of the other operating states will shed some more light.
The second slide shows what happens to the auxiliary and PWM supply nodes when the main outputs are trying to turn on. The math channel shows the voltage across the PWM's supply bypass capacitor. There is nearly 20VPP of noise on the controller's supply and the DC value is barely 10V with some dips below its under-voltage threshold. Not many chips will operate well when their supply has nearly twice as much noise as it does DC, on top of input voltage being barely above the minimum operating voltage. The W12NK80 MOSFET used in this power supply needs at least 8V to switch 6A, but the PWM controller needs to have a supply a few volts higher than that to offset its own gate drive's voltage drop and the voltage drop across external gate drive components, meaning 10V is nowhere near enough for reliable operation.
The final slide shows what happens when the PWM manages to turn on. I forgot to enable the math channel on this one, but if you use a little imagination to extrapolate the voltage between the bottom capacitor voltage and aux/PWM supply voltage, you can see the controller IC's input voltage is still quite messy. If you have trouble imagining this, look at areas where the cyan line and the two others move in opposite directions. Those would be spikes, many large ones at that. With finer time resolution, we can see that the PWM supply voltage is tracking AUX voltage almost perfectly, indicating that the photocoupler has no trouble driving its pass transistor into saturation to feed the main switcher, so we can dismiss the on/off control photocoupler and its pass transistor as potential concerns. We can also see fairly well that the auxiliary voltage is about 20V above the bottom cap voltage on average.
The auxiliary cap definitively needs to be replaced, and given the way the PWM bypass capacitor tracks it, there is a high probability it needs replacement too. If the PWM's bypass capacitor still had even 1µF of its nominal 22µF left, I would expect to see that charge decay over a few microseconds between top-offs by auxiliary peaks instead of momentary glitches.
If this was a normal repair job, I would replace both on the same soldering iron run and ask questions later. But since I am taking a case study approach, I am fixing problems singularly so that I can observe, analyze and comment on causes and effects, one variable at a time.


Rolex Datejust II 116300 Watch | Watchfinder & Co.
Great website, customer service and fast delivery. Would recommend.
Tom was fantastic, he was very helpful in my purchase, the watch came and it was perfect!
Fantastic company and would strongly recommend to anyone looking for a watch. If the watch is not on the website or in the boutique / show room then simply email what your looking for and they will source out the watch you want at a great price. Third time I have used Watchfinder now and Kieran Narain has been a great help each time for all 3 transactions. Great contact to have and recommend him to anyone as he is quick with his responses and always looks to get the best price possibly for the customer.


Brian Youssef was the recipient of a refurbished 2017 Chrysler Pacifica.
PATERSON – After serving three tours of duty in Iraq with the Marines, Garfield resident Brian Youssef has struggled since coming home to New Jersey.
The 33-year-old said he has gone through treatment for post-traumatic stress disorder and bounced around in different jobs.
About six months ago, his used car needed repairs and he had to junk it because he didn't have enough money to get it fixed. Not having a vehicle has made it that much more difficult to get his life on track.
But on Friday, Youssef's transportation situation took a dramatic turn for the better. He received from Dynasty Auto Body in Paterson a 2017 refurbished Chrysler Pacifica, a minivan with 5,000 miles on it.
The donation stemmed from Dynasty's participation in the National Auto Body Council's "Recycled Rides" program, which takes vehicles badly damaged in collisions, fixes them up and provides them to people in need. Participating body shops decide who gets the vehicles. Dynasty makes its donations to military veterans, said the shop's office manager, Anne Kolea.
Youssef and the other recipients all had to go through an application and screening process to confirm their military service, as well as need and ability to handle expenses like insurance and maintenance, Kolea said.
Dynasty does its Recycled Rides projects in conjunction with insurance companies, parts vendors and other auto service companies.
For Friday's announcement of Youssef's gift, local officials held a full-scale celebration at Dynasty's shop on Pennsylvania Avenue. A color guard of public safety responders presented the flag at the start of the festivities, followed by patriotic speeches from political figures amid red-white-and-blue balloons. The event also featured someone wearing a Geico gecko costume, for the insurance company that provided the minivan, which Kolea said had been out of commission since it was in a crash in January 2017.
Among those in the audience was Alex Nieves, another Marine who had gotten a 2011 Dodge Caravan through Dynasty's Recycled Rides back in April. "It really helped me out," said Nieves, who subsequently landed a job as a Bloomfield firefighter.
Youssef said he was wounded in a bomb explosion during his second Iraq tour, which ran from 2006 to 2007. That didn't stop him from returned a third time from 2008 to 2009.
Youssef left the Marines in 2012 and found a glutted employment market.
"I'd go for a job and there would be 30 other applicants," he recalled.
Youssef took day labor jobs for income and then went to school to become a heat-ventilation and air-conditioning technician. But he said many of the HVAC employment opportunities did not provide adequate health protections. For a while, he managed a pizzeria in Ridgewood, but lost that job when the owner sold the business and the buyer cut the payroll, he said.
The Marine's father, Sabit Youssef, said getting the minivan will give his son a boost.


I do not understand picky eaters. You know the ones (maybe you are the one?) The people who eat at five different restaurants and nowhere else (and all the places are chains.) The people who see one unfamiliar ingredient and say, "Eeew!" The kids who will eat nothing but hamburgers and french fries at every meal.
When I first got pregnant with my daughter, I made it a personal goal to make sure she ate well. No weekly Happy Meals, fruits and veggies every day, and there was no way she was going to be a picky eater. I was not going to be the parent at the fancy restaurant who askes the waiter if "the chef can't just make up a grilled cheese sandwich, that's all Little Precious will eat."
Well, we had the kid and reality set in. When you go out to eat most places, all there is for kids is fast food fare--hamburgers, french fries, chicken nuggets, sometimes pasta that turns out to be Kraft Mac-n-Cheese (and the restaurants show no shame in charging $4.50 for something we all know comes in a yellow box for $0.30.) Unless you can afford to pay for another adult entree that you know will barely be eaten, most parents must buckle down and allow that occasional lapse into fast food. Before we knew it, the Tyke was asking for chicken nuggets at every meal.
So I figured that we could at least get some whole chicken breast into her once in a while (what the heck are mechanically separated chicken parts, anyway?) They may be fried but at least I know what every ingredient is. There will be a general recipe at the bottom, but with no measurements--this is one of those "a pinch of this, a pinch of that" recipes.
What you'll need to start is a package of chicken tenders (or chicken breasts that you've sliced into tender shape), buttermilk and hot sauce. I like to marinate the chicken in the buttermilk and hot sauce for a while. The buttermilk gives the tenders a nice tangy flavor, as well as keeps the meat moist.
Pour some buttermilk into a plastic bag set into a bowl (be sure not to use a plastic bowl, as plastic can harbor salmonella germs.) I like the plastic bag method, especially since I'll be making something of a mess later on. This is one less thing I'll have to clean. I didn't measure the buttermilk--just added enough where all the chicken could soak. I made sure to leave a cup for pancakes later in the week...but that's another post.
Add liberal dashes of your favorite hot sauce. It gives the tenders a little "kick".
Toss in the chicken and seal the bag. I just let it hang out while I do other things--in this case, I made some homemade mayo and then turned that into honey mustard, to dip the tenders in. Recipe for mayo and honey mustard to come tomorrow!
While the tenders continue to marinate, get the breading station ready. In one bowl, I simply put in breadcrumbs. You can use any kind you like--here I actually have a mixture of regular breadcrumbs, plain panko breadcrumbs, and Italian seasoned breadcrumbs.
Okay, I'll admit it. Basically I keep forgetting which kind I currently have in my storage jar and, in the interest of saving space, dump whatever the newest box of breadcrumbs I buy in there so I won't have the jar AND the box. After dumping the new into the old, I usually realize that what I just bought is not the same as I had before. Which is why there were plain breadcrumbs on the bottom, plain panko breadcrumbs in the middle, and Italian ones on top. Ah well, breadcrumbs are breadcrumbs, right?
In the other bowl is just flour seasoned with salt, pepper, and a little cayanne. Like I said, I like it a little spicy! Again, no measurements but try to use more than you think you'll need. It's a huge hassle to stop what you're doing and get out more flour or whatever. You'll see what I mean in a second.
Finally, the plate on the far right is just a plate with a cooling rack set over it. The dredged chicken will sit on there, allowing the breading to dry out a bit. The drier the breading is, the crispier your nuggets will be. And I'm telling you now, these boys are crispy.
Grab a piece of chicken out of the bag and shake off the excess buttermilk. Coat the piece in the flour. Dunk the chicken back into the buttermilk and then move it over to the breadcrumbs.
Coat the chicken in the breadcrumbs, and then set on the cooling rack to dry.
See what I mean about not wanting to stop to refill your flour and breadcrumbs? The pros say to have a "wet" hand and a "dry" hand so situations like this don't arise, but the pros also don't have a food blog that they have to take pictures for themselves.
While the nuggets are drying out, your oil should be heating. I used a couple inches of oil in a pot. Despite what you might think, you don't need quarts and quarts of oil to fry (unless you're frying a lot of something--or deep-frying a turkey!) I could have used a larger pot (the one I used only fit a couple nuggets in at a time) but then that would have required more oil, and frankly I hate the mess of cleaning up used oil. The less I have to get rid of afterwards, the better.
On the left is a plate with newspaper and another cooling rack on top of it. The newspaper is there to soak up any extra grease. Just one more trick to getting supercrispy nuggets.
Check out those irregularly-shaped nuggets. I'd complain about quality control, but I could have cut those big ones down a little more. I have to admit, though, they were still slightly frozen when I tossed them in the buttermilk and I kind of hoped they were two smaller pieces stuck together. Ah well. That just means my husband gets to eat the "manlier-sized" nuggets.
However you decide to heat your oil, once it's ready you can toss the nuggets in. Make sure no kids or pets are in the area...that stuff spatters.
The largest nuggets took about 9 minutes to cook; the smaller ones were closer to 7. When they're done cooking, set them on the cooling rack on top of the newspaper. Make sure your oil stays at the same temperature. If it gets too cool, you'll just be poaching your chicken and it'll turn into a greasy mess. If it gets too hot, it'll be burned on the outside and raw on the inside.
I paired mine with some frozen french fries I baked in the oven (who wants to heat two different pots of oil?) and my homemade honey mustard that I made earlier.
You could tweak this recipe any number of ways. You could use fish or pork. You could replace the buttermilk with beer for a beer batter. You could use different spices in the breading--cajun spices or southwestern spices. You could leave out the hot sauce or add more. You could use different breadcrumbs. If you're gluten free, you could use rice flour. You could probably even use tofu (although I'd probably bake it instead of fry.) So many possibilities!
Marinate chicken in buttermilk for at least 30 minutes.
Dip chicken into flour and coat lightly.
Dip chicken back into buttermilk.
Allow chicken to rest at least 10 minutes to dry out coating.
Fry chicken until cooked through (at least 155F; chicken is recommended to be at 165F but take into account that the chicken will continue to cook a bit after you take it out of the oil.) Drain on cooling rack set over newspaper or paper towel.
I'll just tempt you with pictures of the honey mustard. You'll have to wait until tomorrow to get the recipe!


text:
(Redirected from Flansham)
7.9 km2 (3.1 sq mi)
452/km2 (1,170/sq mi)
Bognor Regis and Littlehampton
http://www.yaptonpc.gov.uk/
50°49′12″N 0°36′40″W / 50.81999°N 0.61121°W / 50.81999; -0.61121Coordinates: 50°49′12″N 0°36′40″W / 50.81999°N 0.61121°W / 50.81999; -0.61121
Yapton is a village and civil parish in the Arun District of West Sussex, England. It is centred three miles (4.8 km) north east of Bognor Regis at the intersection of the B2132 and B2233 roads.
The parish of Yapton lies on the coastal plain south west of Arundel, between the South Downs and the sea. St Mary the Virgin parish church, 13th century or earlier in origin,[2] is in the centre of the village. It houses a twelfth-century font. The village includes Yapton C of E Primary School.
Other settlements in the parish include Bilsham and Flansham. Bilsham Chapel is a deconsecrated former chapel of ease to St Mary the Virgin Church; it dates from the 13th–14th century but fell out of use in the mid-16th century. The building is now a house.[3]
3 Notable residents
Main article: London to Portsmouth canal
The disused Portsmouth and Arundel Canal which linked nearby the River Arun to Chichester Harbour, runs through the village. This was built primarily on the financial case it would be a safe inland shipping route between London and Portsmouth avoiding the then risk of attack in the English Channel by the French. Throughout its lifetime, 1823-1855 - a time when the Wey and Arun Canal was open (having opened 7 years earlier and having closed in 1871) - the route was used for transporting agricultural goods and munitions, for instance, from Chilworth and Hanworth gunpowder works and to/from the military docks on the lower Thames in the Royal Borough of Greenwich.
An electoral ward in the same name exists. This ward stretches South East to Climping with a total population of 5,926 at the 2011 Census.[4]
Notable residents[edit]
Duncan Goodhew, the Olympic swimming maestro was brought up in Yapton. There is a residential road, Goodhew Close, named after him. Sculptor Ginger Gilmour works from a 15th-century farmhouse[5] near Yapton,[6] where she has created several studios.[5] The poet Andrew Young lived in Yapton after he retired in 1959.[7]
^ British History Online; Yapton
^ Hudson, T. P. (ed) (1997). "A History of the County of Sussex: Volume 5 Part 1 – Arundel Rape (south-western part including Arundel). Yapton". Victoria County History of Sussex. British History Online. pp. 245–261. Retrieved 27 November 2011. CS1 maint: extra text: authors list (link)
^ a b Shewry, Brian (19 August 2004). "Ginger's quest is where angels are always pleased to tread". Littlehampton Gazette: 31–32.
^ "Sculpture attack casts shadow over arts festival". West Sussex Gazette (7896). 23 August 2006.
^ "About Andrew Young | The University of Edinburgh". www.ed.ac.uk. Retrieved 30 September 2015.
Wikimedia Commons has media related to Yapton.
Local Heritage Initiative
Retrieved from "https://en.wikipedia.org/w/index.php?title=Yapton&oldid=922376714"


Wrapped in a luxurious leatherette, our Alisa Bed is a gorgeous platform bed with style and the appropriate flash. An elegant square pattern is stitched across the headboard, adding a traditional class to Alisa's modern lines. Black wooden block feet complete the design. Constructed with a bowed and slated mattress support, Alisa provides generous comfort and durability without the need for a boxspring. Available in Queen and King sizes, and either white or black leatherette. Mattress and bedding not included.


Size doesn't matter. Safe is how we move!
You can rest assured that our professionals will move your prized possessions with the same level of care and attention that you would. We understand that an item's sentimental value makes an item priceless. No matter the size, shape, or weight, we care about your items. We will take care of all your moving needs. From furniture to fine art, our movers are fully equipped to handle any job with relative ease. No move is too big or too small for our team. Thanks to our expertise, we guarantee safety and efficiency for every move we perform.
hour charge (like most big moving companies). We'll even rearrange your furniture and personally hang up pictures.
To check how we can make your life easier, remember to discuss all moving needs, from packing to TV installation.
Our moving system protects your items, and moves them safely in our small, environmentally friendly vehicle that helps save unnecessary vibrations during driving. Our movers will wrap furniture with moving pad. No matter how fragile or delicate your item is, we will wrap is and make sure that your possession will complete the move safely.
We develop a mini transportation system design for San Diego moving needs, San Diego movers will show up with a flatbed small vehicle so the moving process will be smooth and efficient. Not to mention we don't have any double drive time charge, no minimum hours charge either.
So if you looking for a small move in San Diego, we are your team.
SoCal is such a big area so it hard to meet your small needs.
Our moving system meet your small moving needs so you can get small move for a small price.
Call us to consult your moving needs 858-848-0121 We are here to help you have a stress-free Move and ship Your belongings safely to their new home!


text:
What's on The Lens
Live film scores
The story of the moving image
Stories & Ideas
Collections & Preservation
The Lost Thing (2010)
Shaun Tan on The Lost Thing
CraftExhibitionShort filmWatch
ACMI
Your museum of screen culture
Not just a hunk of metal
When Shaun Tan drew The Lost Thing for his award winning Children's book, he had a very clear idea of how the Lost Thing would move and sound. So when it came to creating the animation Shaun had pages of pencil drawings and detailed notes for the animators.
"It was a crazy, hermit crab, teapot, tentacled thing that somehow had to be endearing and lovable." With no face to work with, the animator created a range of expression and emotion through body movement. You can tell exactly if the The Lost Thing is sad or happy or scared or excited by the way it walks.
Some embedded content has been omitted:
The Lost Thing About the Lost Thing by ACMI (Australian Centre for the Moving Image)
View here: https://www.youtube.com/watch?v=iIc4pBTAys4
A delightful insight into Shaun Tan's creation of the Lost Thing:
Shaun Tan draws The Lost Thing by ACMI (Australian Centre for the Moving Image)
View here: https://www.youtube.com/watch?v=a_xoudh_cdU
Our museum is currently closed until 11 Feb 2021
See what's on
Films | On demand | Exhibitions | Education | Shop
Fed Square, Melbourne, Australia
ACMI acknowledges the Traditional Owners, the Wurundjeri and Boon Wurrung people of the Kulin Nation, on whose land we meet, share and work. We pay our respects to Elders past and present and extend our respect to Aboriginal and Torres Strait Islander people from all nations of this land. Aboriginal and Torres Strait Islander people should be aware that this website may contain images, voices or names of deceased persons in photographs, film, audio recordings or text.


Is it time to refresh your Travel Management program?
Then it is time to meet with the GlobalStar Travel Management team at the upcoming GBTA convention in San Diego. We will be exhibiting with GlobalStar Partners from all over the world and we would welcome the opportunity to discuss how we can refresh your travel management program. We will be exhibiting in booth #3205 and we have a great present for all visitors who are interested in a refresh.
For a personal refresh, we are ready to serve you one of our famous GlobalStar cocktails from all over the world. Please feel free to schedule a meeting with us clicking this link or otherwise we look forward to seeing you in our booth at your convenience.


The D-RLC3 is designed to directly interface with RDL® control modules which feature a 0 to 10 Vdc control input utilizing an external 10 kΩ resistive control, such as the ST-VCA3, RU-VCA2A and RU-VA6A voltage controlled amplifiers. The D-RLC3 has four momentary pushbuttons, each corresponding to one of four levels selected.
In a typical installation, the D-RLC3 is wired to an RDL VCA. The D-RLC3 runs from the same power supply as the VCA. When power is applied, the D-RLC3 defaults to the NORM level. One higher level, HIGH, and two lower levels, LOW and MIN, are then available for selection. When the D-RLC3 is set to the HIGH position, the VCA is at maximum gain. The NORM, LOW, and MIN levels are each adjustable by the installer using trimpots on each side of the D-RLC3´s body. Once installed, the adjustments are concealed from the user.


Crumbed chicken breast topped with Napoli sauce, smoked ham, mozzarella cheese.
Tender grilled chicken breast with a creamy sauce of sauteed mushroom, bacon, caramelized onion, honey mustard butter.
Twice cooked tender pork ribs in a homemade smokey bbq sauce, coleslaw.
Sauteed seafood of king prawns, calamari, scallops, crab meat & fresh seasonal fish in a creamy coconut curry sauce, baby spinach and roasted pumpkin.
Double decker sandwich consisting of lettuce, tomato, chicken, crispy bacon, egg.
Oven cooked beef lasagna with Bolognese sauce.
Homemade tasty beef meat balls with sauteed baby spinach, capsicum, onion, sliced mushroom in a Bolognese sauce.


Eight years ago, Rob was inspired by that month's Bon Appetit Magazine. An unsung cut of beef was being lauded as the next big thing in barbecue - the beef brisket! Ever the tinkerer, Rob ordered a slab from our local distributor and, being the Cercese that Rob is, he could not leave well enough alone and stick with the original scripted recipe.
Cover dry rubbed brisket in plastic wrap and store in refrigerator for 48 hours. When ready to cook, preheat grill to 200 degrees Fahrenheit. Remove brisket from refrigerator, discard plastic wrap and place in an aluminum pan uncovered with fat side up.
Brisket grilling requires indirect heat. (Depending on your grill, this is the most crucial and difficult step. Our grill has three burners so we simply light one of them.) Cook uncovered pan of brisket for four hours between 200 and 225 degrees keeping grill door closed. After four hours, remove brisket from grill and pan and discard pan juices. Wrap brisket tightly in aluminum foil (caution - use oven mitts for this step) and return to pan.
Brisket returns to grill at same temperature for four more hours. At that time, remove pan from grill, shut grill down and leave pan sitting on the counter for 20 minutes. You may choose at this time to trim exess fat from the top of the brisket or leave it intact - the cooking process will have rendered much of it down and many guests prefer their piece still marbled. Once meat has rested, slice from point side to trunk in 1/4 inch or less slices.
The dry rub alone provides the flavor - no sauce required. Serve with fresh homemade coleslaw for a little cold crunch and on fresh hard rolls. Don't skimp on the rolls; buy them the morning that you plan to serve the brisket! - you will embarass yourself in front of Italians if you serve old bread!
This week at our store, La Molisana Sausage in Waterbury, we sold beef brisket for $3.59 per pound. This recipe serves ~14 people (that are good eaters!) thus the cost per person is ~$4 for an impressive and hearty summer meal. Give Rob a call at 203-574-1272 and place your brisket order. Send us your feedback on this recipe or share your version to let us know how you like it.


text:
Q: Express Routing Exercise returning weird error i don't know if the approach in general is very DRY but i toyed around with if statements for a solution for the second part of this exercise on colt steeles wdbootcamp:
#Express Routing Assignment

1. Create a brand new Express app from scratch
2. Create a package.json using `npm init` and add express as a dependency
3. In your main app.js file, add 3 different routes:

Visiting "/" should print "Hi there, welcome to my assignment!"
==============================================================
Visiting "/speak/pig" should print "The pig says 'Oink'"
Visiting "/speak/cow" should print "The cow says 'Moo'"
Visiting "/speak/dog" should print "The dog says 'Woof Woof!'"
==============================================================
Visiting "/repeat/hello/3" should print "hello hello hello"
Visiting "/repeat/hello/5" should print "hello hello hello hello hello"
Visiting "/repeat/blah/2"  should print "blah blah"

If a user visits any other route, print:
"Sorry, page not found...What are you doing with your life?"

My first approach for a Solution
var express = require("express");
var app = express();

app.get("/", function(req, res){
   res.send("Hi welcome");
});

app.get("/speak/:animal", function(req, res){

   var animal = req.toString();

   if (animal.toLowerCase() === "pig") {
   res.send("the pig says oink");
   }
   else if (animal.toLowerCase() === "dog") {
   res.send("The dog says woof");
   }
   else if (animal.toLowerCase() === "cow") {
   res.send("the cow says moo");
   }
});

app.listen(process.env.PORT, process.env.IP, function(){
    console.log("Server has started");
});

This gives me the following "error" on page

    <meta charset='utf-8'>
    <title>Error 502 - Bad Gateway</title>
    <link rel="stylesheet" type="text/css" href="https://cdn.c9.io/errors/style.css" />

Error 502 - Bad Gateway

Please click here to try again, if the issue persists please contact support

      Status Page |
      Support |
      Dashboard |
      Home

what does this cause to happen? Is this a serverside problem? How can i check if my code is running when c9 is down? I really want to solve this "on my own" and not just look up the solution.


text:
[![Dependency status][dependency-badge]][dependency-url]
[![DevDependency status][dev-dep-badge]][dev-dep-url]
[![Licensing][license-badge]][license-url]
[![Release][release-badge]][release-url]
[![Issue tracking][issues-badge]][issues-url]

[dependency-badge]: http://img.shields.io/david/dstroot/express-loggly.svg?style=flat
[dependency-url]: https://david-dm.org/dstroot/express-loggly

[dev-dep-badge]: http://img.shields.io/david/dev/dstroot/express-loggly.svg?style=flat
[dev-dep-url]: https://david-dm.org/dstroot/express-loggly#info=devDependencies

[license-badge]: http://img.shields.io/badge/license-MIT-blue.svg?style=flat
[license-url]: #license

[release-badge]: http://img.shields.io/github/release/dstroot/express-loggly.svg?style=flat
[release-url]: https://github.com/dstroot/express-loggly/releases

[issues-badge]: http://img.shields.io/github/issues/dstroot/express-loggly.svg?style=flat
[issues-url]: https://github.com/dstroot/express-loggly/issues

express-loggly
===================

Express middleware to send JSON formatted logs to Loggly from an Express application.  Use this logger to send all web traffic to Loggly for capture and analysis.  It uses [node-loggly](https://github.com/nodejitsu/node-loggly) by nodejitsu.

Why JSON Logs? See:

https://journal.paul.querna.org/articles/2011/12/26/log-for-machines-in-json/

http://blog.nodejs.org/2012/03/28/service-logging-in-json-with-bunyan/

## Install

```
npm install express-loggly --save
```

## Usage

```js
var express = require('express')
var logger  = require('express-loggly')

var app = express()

// Setup Loggly configuration
var config = {
    token: 'your-really-long-input-token',
    subdomain: 'your-loggly-subdomain',
    tags: ['tag1', 'tag2', ... 'tagN']
};

// Sequence of use() matters!
// Put this *after* session and bodyParser

app.use(logger({ loggly: config }));
```

### Options

#### immediate

Write logs on request instead of response. This means that a requests will be logged even if the server crashes, but data from the response cannot be logged (like the response code).  Use like this:

```js
app.use(logger({
  immediate: true,
  loggly: config
}));
```

### Release Notes

Creating a release: (When everything is tested and ready for a new npm release)

* Bump the version in your `package.json` file.
* Commit that change with a message like "Release 0.0.5".
* Push commit to Github
* Create a new release tag:
  * **Listing Tags:** type `git tag`
  * **Creating Tags:** Use annotated tags - they're checksummed; contain the tagger name, e-mail, and date; have a tagging message; and can be signed and verified with GNU Privacy Guard (GPG). `git tag -a v0.0.5 -m 'Release 0.0.5'`
  * **Pushing Tags to Github:** By default the git push command doesn't transfer tags to remote servers. You have to explicitly push tags. You run git push origin [tagname].  `git push origin v0.0.5`
* Use the Github GUI to create a release from the tag.  Confusing, yes? Not sure why Github separates tags from releases but they do and using the web site is easy: `https://github.com/dstroot/express-loggly/releases`
* Publish the new version to npm: `npm publish https://github.com/dstroot/express-loggly/archive/0.0.5.tar.gz --tag 0.0.5`

## License

The MIT License (MIT)

Copyright (c) 2014 Daniel Stroot dan@thestroots.com

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

redpajama_set_name: RedPajamaGithub


What is the difference between primary, secondary,tertiary and … ... Search difference between primary crusher and secondary crushers to find your need.
Crusher: crusher primary,secondary,tertiary difference between primary secondary and tertiary crushers . Difference Between Primary And Secondary Crusher.
Overview of the protein structure. Proteins are macromolecules and have four different levels of structure – primary, secondary, tertiary and quaternary.
What is the difference between a primary, secondary and tertiary industry? ... What are the similarities between primary, secondary, and tertiary sectors?


text:
Press Releases – Indiana Foster and Adoptive Parents
Organization to systematically connect parents and coordinate their input to influence sensible and effective foster and adoptive policies, education, and training
Brazil, Indiana, 10/30/17 – Coinciding with National Adoption Month, Kristi Cundiff, founder of the popular Indiana Foster and Adoptive Parents Facebook Group, announces the launch of Indiana Foster and Adoptive Parents Resources and Advocacy Group, Inc., (IFAAP) as a 501(c)(3) nonprofit organization.
"As a parent who has opened my heart to many foster children, I have firsthand experience with the joys and challenges of fostering," explains Kristi. "Without good support and effective policies, it can be difficult to consistently provide stable, loving homes that will make the most of our children's potential."
While there are several Indiana organizations that already help foster and adoptive children and families, none represents the unique perspective of foster and adoptive parents.
"Foster parents have limited parental rights, so we're often overlooked," notes Kristi. "Yet we have the most intimate view of when and how the system works – or doesn't – so we're uniquely positioned to bridge gaps and solve problems to ensure the best outcomes for Indiana's children."
IFAAP has already identified its 2018 priorities, based in part on surveys Kristi has conducted with IFAAP Facebook Group members. Those priorities include: 1) Invest & Protect, which focuses on childcare financial assistance and car seat safety and certification, 2) Trauma Informed Care, which focuses on TIC legislation, training, and education for all people who work with children, and 3) Follow the Fed, which focuses on ensuring adherence to federal policy, including the SAFE Families Act, Title IV-E Adoption Subsidy Guidelines, and Vocational Rehabilitation.
"Indiana's foster children belong to all of us, and they need us," Kristi emphasizes. "Even if you aren't able to open your home to children, you can make a big difference by supporting us financially, volunteering your time when needed, and joining us on social media."
The IFAAP website can be found at FosterAndAdoptiveParents.org. You can reach Kristi Cundiff at Kristi@FosterAndAdoptiveParents.org, and request to join the IFAAP Facebook Group by searching on Indiana Foster and Adoptive Parents.
About Indiana Foster and Adoptive Parents Resources and Advocacy Group, Inc.
The Indiana Foster and Adoptive Parents Resource and Advocacy Group, Inc. (IFAAP), originated as a closed Facebook Group started by Kristi Cundiff, who wanted a place where foster and adoptive parents in the State of Indiana could network and get support. Today, there are more than 5,200 members, and the group continues to grow. With this growth, Kristi saw an opportunity to do even more for Indiana's foster families by formalizing the group's work as a nonprofit. As a nonprofit, IFAAP will offer more coordinated advocacy and support to foster and adoptive families.
Vernon – Indiana Foster and Adoptive Parents
Facebook – Indiana Foster and Adoptive Parents


text:
require "grape_swagger_ui/engine"

module GrapeSwaggerUi

  mattr_accessor(:url) { '/api/swagger_doc' }
  mattr_accessor(:doc_expansion) { 'none' }
  mattr_accessor(:dom_id) { 'swagger-ui-container' }
  mattr_accessor(:supported_submit_methods) { ['get', 'post', 'put', 'delete', 'patch'] }
  mattr_accessor(:sorter) { 'alpha' }
  mattr_accessor(:authenticate_method) { -> { puts 'No Authentication Required.' } }
end