So you're handy with power tools and have a pile of cash burning a hole in your mattress. Flipping real estate may be just the ticket to grow that pile into an Everest of dough!
However, before you start attending auctions or scouring foreclosure sites for deals, you should know that flipping has its own colorful, and, to beginners, puzzling slang. Our Learning the Lingo series can help you look like you're in the know.
Read on to understand the difference between a dollhouse and a haircut and beyond.
Yeah, this one you already know, but it's the starting block for most flips. Distressed properties have fallen on hard times and are usually in the process of foreclosure. Since the original owner hasn't paid the mortgage for some time—maybe a long time—the bank or lender has taken over. It generally has one goal: sell the place and sell it fast. That's why distressed properties are priced far below market value. Hello, bargain basement!
This is the primo list of leads generated by property data companies on homes where owners are 30, 60, or 90 days late in paying their mortgage.
What's important here is that the property is in pre-foreclosure andnotice of default, or NOD, has not been filed; therefore, the property is not public knowledge yet. If you want to beat other rehabbers to the property, these lists give you a sneak peek at which homeowners might want to throw in the towel so you can pounce. They give you a competitive advantage in the marketplace. They also usually cost money, often priced per lead.
This is the price people will pay for the property after you fix it up. It's your profit margin! It's what you're working for, and it's the key metric in your investment strategy. You need to do a realistic calculation of what the home could be worth based on the amount of work you plan to do and the comparable homes in the area. So if you find something that looks like junk but has great bones and is located in a desirable neighborhood, the after-repair value, or ARV, might just sound phenomenal and you should probably plunk down an offer.
It's a minor fixer-upper. Barbie herself would be proud to live there because it needs only cosmetic attention—some new paint and maybe a few lighting fixtures. There's an absence of the deeper problems (foundation, plumbing, electrical) that would require lots of elbow grease and hard cash in order to get the place up to snuff on the market.
You like the no-fuss sound of a dollhouse? This property is even more sales-ready. Just take out the trash and mow the lawn before planting that "for sale" sign, and you're good to go!
In the same way wirehaired pointers flush out pheasants, these professionals hunt down distressed properties whose owners might be desperate to sell. If they smell blood, bird dogs (also called "deal scouts" or "real estate jobbers") then sell these leads to frequent or professional flippers to swoop in and close the deal.
Flippers often use other people's money, or OPM, to curb their own financial risks. Unless you're fully confident in your flipping skills and flush with cash, OPM can make the difference between an intriguing side venture and a do-or-die investment.
Hard-money lenders give loans to flippers in a fraction of the time it takes to get a traditional mortgage. They are not banks, but rather private businesses or individuals. And yes, they're expensive, with interest rates well in the teens. But at an auction, when flippers need cash quickly, a hard loan can serve as a bridge until they can secure longer-term financing—or just flip the house and pay their lender back.
This is a property flipper on human growth hormones. This professional buys a distressed property and then immediately turns around and sells it as is to another flipper. Sometimes these guys work so fast they don't actually close on the initial sales contract; they just agree to buy the property, but before closing, find a buyer and simply assign the contract of sale to this next guy in line. It's great because wholesalers pay nothing but rake in a profit. That said, you'd better know what you're doing and how to spot properties with real potential.
This is a more relaxed flipper, someone who approaches it as a hobby or a part-time second income. Usually these flippers invest their sweat equity (see below) on, well, you guessed it, the weekend.
This is the energy and effort flippers put into buying a property, fixing it up, and selling it. With any luck, it does not involve tears, blood, or hospital visits due to a malfunctioning nail gun. A bit of actual sweat is OK.
This is the ridiculously rapid-fire patter of an auctioneer at a foreclosure auction. It takes these guys years of practice. The National Auctioneers Association hosts an annualInternational Auctioneer Championship in which contestants are scored, among other things, on clarity, voice control, speed, rhythm, and voice expression.
Bene is an abbreviation for "beneficiary." When a distressed property doesn't sell at a foreclosure auction, it goes automatically to the bene—the bank or lender, which will typically then just put it up for sale as a foreclosed property and hope for the best.
Can Paint Color Increase Your Happiness?
Learn how the color choices in your home can increase your well being.
When you think of the word "wellness" you may think of a trip to the spa, or the mountains, or maybe wellness is your weekly yoga class. But can wellness be incorporated into your home? What does that mean, exactly? The Random House Dictionary defines wellness as "the quality or state of being healthy in body and mind,especially as the result of deliberate effort." Wellness and well-being can be thought of as the act of purposefully creating a state of feeling good.
You might already have a wellness room in your home without knowing it. Perhaps it's the kitchen or the garden where you feel creative, connected and inspired. Maybe it's the oversized tub where you relax and unwind at the end of the day. It might even be that cozy nook by the window where you love to sit and read. At a recent trip to Heimtextil, design experts from all over the world and across multiple disciplines designed a special exhibition called Well-Being 4.0. They focused on that which makes us feel protected,energized, nourished and enriched. Given these descriptives, you'd be amazed at how right color strategy in your home can influence your well being and help create a stronger sense of wellness for you and your family.
Whether you are aware of it or not, color plays a huge part of your life. From the clothes you put on in the morning, to the foods to buy at the grocery store, to the brands you reach for in the pharmacy, color is influencing you constantly. In fact, brands hire color experts when launching new products or when entering a new marketplace. Experts know that the colors of the package can increase, or decrease, sales.
The colors you pick for decorating your home, inside and out, influence your overall wellbeing too. You've probably purchased a colorful trendy paint you saw in a magazine only to realize that in person the color gives you a headache, or makes the room feel dark and depressing. Your very perception of color can really make or break a room, so to speak.
Frank H. Mahnke was a founding member of IACC (International Association of Color Consultants/Designers), which aims to bring color education and expertise into the world of design, including interior design. The pyramid above shows how much influence certain factors, like cultural influences, may have on your love of certain colors.
The strongest influence, Mr. Mahnke describes, is our biological reactions to a color stimulus. Think of the luscious, bright red colors of sweet berries that our ancestors foraged for quick bursts of energy.
Less influential than that but still very strong is our collective unconscious, which would include the examples of orange and red, which signify the colors warmth and security of a roaring fire.
Conscious symbolism – associations is like how children always color the sun yellow, and that we still equate that color with a sunny point of view.
Cultural influences and mannerisms refer to the colors that become symbolic within our own distinct cultures. The color white, for example, represents purity in the U.S., and is used for wedding dresses and baptismal gowns. However in Japan, white is the cultural symbol of mourning, and people wear that color when attending funerals.
The influence of trends, fashion and styles are less important but still may influence our color choices. This is easy and obvious to see, particularly when a photograph of a movie icon on the red carpet spawns a whole industry of new dress designs.
Finally, our own personal relationship to color, although the least influential, is still important. If you've always hated the color of orange because you had a bad experience with something that was a dominant color orange in your memory bank, you may never like that color.
Of course, understanding this pyramid of influence is just one part of why we like certain colors in our home. But it's an important idea to grasp when thinking about how certain colors in your home can make you feel protected, energized, nourished and enriched – in other words, make you feel well.
Color is merely our perception of light, and as we age our perception of color, or our ability to remember color, may change. Hospitals, for example, are experiencing the effect of color with patients suffering from memory loss. In fact, memory and color are closely intertwined. If you don't find this compelling think about how large parking garages use color to influence your ability to remember where you've parked your car. Our brains can only pay attention to small amounts of information at a time, that is exactly why brands rely upon color to impose their brand on your brain.
The above example by design company Printsome shows just how powerful color can be on our memories. Check out their other brand color-swap examples; what does your mind think when the Starbucks logo is illustrated in Dunkin' Donuts colors? Does your mind switch brands?
Despite this information about color and memory, Ms. Seybold also mentioned that it may be hard to describe why, exactly, we like a certain color more than others. Studies have shown that when shopping, 93% of consumers place visual appearance over all other factors. 85% of shoppers say color is the primary reason for selecting a particular product.* Within seconds our brains can recognize a brand before we even register the letters or logo.
So how does all of this help you choose the right colors for your home? How does this help you create a state of well being in your living room, kitchen or home office? It's important that you go with your gut (your biological reaction) when selecting linens, paint colors or upholstery. Choose colors that makes you and your family feel good and remember that in a room full of furnishings you have many opportunities to influence the overall color palette. You may choose earthy neutrals for your long term investments like carpeting or flooring, and you may want to experiment with "new" colors in the form of inexpensive accessories that can quickly be replaced.
Leatrice Eiseman, Executive Director of the Pantone Color Institute, head of the Eiseman Center for Color Information and Training, and author of nine books on color, understands that feeling good about your interior colors is not necessarily about choosing one color over another. It's important to play with the right shades and tones for your own home. "It's a mistake to simply rely on paint swatches," she says about choosing the right color paint for your walls. Test out paint samples on the wall or on sample boards and live with it for awhile. Take note of how the color changes with the light of day and how it works with the bounced light from your furniture. Ms Eiseman recalled that when living in sunny Los Angeles she loved a particular shade of yellow she chose for her home. When she moved to the Northwest, she still kept yellow as a primary color but had to adjust the shade in order for it to work well with the grey lighting of the cloudy Northwest.
Take your time. You should never feel rushed when buying fabrics, linens, furniture, carpeting or any interior furnishes for your home. Use Pinterest to create color boards or cut out magazine images. Putting your gut feelings down on paper is the best way to gain focus and identify how you respond physically and mentally to particular colors or color combinations. Using a professional interior designer can help speed up the process however you still need to know what colors you do, and do not, like. Read more tips for working with an interior designer.
Always bring home swatches. Most furniture companies have fabric samples that you can bring home. Not only is this a great way to see how your family reacts to the color, it's also a great way to see how it works within the room. Read more about samples and swatches here.
Don't choose paint colors in the store. The biggest mistake is to pick out your paint chips in the store without testing it out at home first. Even if you have to purchase a small quantity of paint to test at home, it's worth the money to paint part of your wall or paint a sample board. This same idea should be used when selecting wallpaper as well.Read more of our painting tips here.
Hire a color consultant. Color consultants are definitely a worth-while investment, especially when you need to make a lot of color decisions at once. If you are repainting the whole home, for example, or building a new home, this is a great opportunity to learn more about the colors you love and how to settle on the correct shades. Find an expert here.
The holidays are over but the cold weather is still here…..at least for some of us. My Christmas decor is back in boxes and all of the greenery has been tossed. So now what? Do we make our spaces all springy? Or do we use the cold weather to create cozy homes? I like to call this "cold weather decorating". I have a few easy tips for you to create a warm space for this chilly season.
1. Use throw pillows, not only for added textile but to add warmth and comfort. Splash them around your home, using them on sofas, chairs, beds, window seats, benches and even on the floor.
2. Magazines and books can immediately give the sense of cold, rainy days that require lots of blankets, hot chocolate and hot fireplaces. Display them by stacking them on the floor, on tabletops or displaying them on shelves. Create a more organic feel by mixing and matching sizes and colors.
3. Rugs ALWAYS warm up a space. Choose a rich, textured rug for your powder room. Your guests will love it!
4. Wood accessories bring a sense of rustic, warm moments. I love pairing vintage wood pieces with more modern, streamlined pieces.
5. Display your silver collection with pride. All of that gorgeous old silver compliments the cold, winter months and creates a masterpiece.
6. Sheepskin is an obvious choice for creating cozy spaces. You can display them on chairs, sofas, benches and even in front of fireplaces.
7. If you want to bring some practical warmth to your bed as well as adding some style, a simple quilt will do the job!
Happiness in the home can be a part of the ROI, but other cost vs. value factors vary by region and even by room.ROI, as defined in Remodeling's 2015 Cost vs. Value report, can be broken down as the percentage of the estimated average cost of a renovation project that is projected to be recouped in resale value, as aggregated from real estate and appraisal estimates. Let's take a look at some of the most common renovation projects and how their ROI breaks down.
According to Huffington Post's 10 Most Popular Remodeling Projects Across the Country, the most common remodeling job request projects in the United States are in the bathroom. A midrange bathroom addition cost, a complied in Remodeling's Cost vs. Value report, in 2015 was estimated at $39,578. The ROI was estimated at 57.8 percent. For those needing a bathroom remodel, the cost averaged $16,724 with an ROI of 70 percent. Upscale additions and remodels naturally went up in cost, but the ROI didn't quite hit the level of a midrange upgrade, with 58 percent and 59.8 percent, respectively. Bathroom remodeling projects that were big in 2015, according to Forbes, included custom vanities, feature floor tiles, bigger showers and plant life.
Kitchen remodel job requests accounted for 69 percent, the second most common in the U.S. Major midrange remodel costs in 2015 averaged at about $56,768 with an ROI of 67.8 percent, while minor remodels saw an ROI of 79.3 percent and a cost of about $19,226. A major upscale remodel could cost upward of $113,097 in 2015, with an ROI at 59 percent. According to My Home Ideas, trends in 2015 included built-in coffee centers, dual-fuel ranges, Italian cooking gadgets, designer dishwashers and wine refrigeration.
Not all projects, of course, are room-centered. Window/door replacement accounted for 44 percent of home remodeling job requests in 2015. This included window replacement, entry door replacement and steel, with ROIs of 72.9 percent, 78.8 percent and 72 percent, respectively. Finished basements also were high on the list, as complied by House Logic, with 27 percent of remodeling job requests. Coming in with an average cost of $65,442 in 2015, the ROI on these projects was 72.8 percent.
Additionally, home remodeling job requests vary by U.S. region. In the East-North Central (Wisconsin, Illinois, Indiana, Michigan, Ohio), the midrange top five requests include entry door replacements, garage door replacements, manufactured stone veneers, vinyl window replacements and vinyl siding replacements. The upscale top five requests include fiber-cement siding replacements, garage door replacements, vinyl window replacements, foam-backed vinyl siding replacements and wood window replacements. In the East-South Central (Alabama, Kentucky, Mississippi, Tennessee), the midrange top five requests were much of the same, however an upscale request of fiberglass grand entrances made the cut.
The Middle-Atlantic (New Jersey, New York, Pennsylvania) followed suit, along with many of the same requests in the Mountain area of the nation (Arizona, Colorado, Idaho, Montana, New Mexico, Nevada, Utah, Wyoming). New England home owners (Connecticut, Delaware, Maine, Massachusetts, Rhode Island, Vermont) in the midrange chose steel entry door replacements, manufactured stone veneers, garage door replacements, vinyl siding replacements and wood deck additions as their top five. Top upscale requests included garage door replacements, fiber-cement siding replacements, foam-backed vinyl siding replacements, vinyl window replacements and wood window replacements.
Midrange top five requests in the Pacific region (Alaska, California, Hawaii, Oregon, Washington) mirrored New England, along with the South Atlantic (Maryland, Virginia, West Virginia, South Carolina, North Carolina, Georgia, Washington, D.C., Florida) region and the West North Central (Minnesota, Missouri, Iowa, North Dakota, South Dakota, Kansas, Nebraska) region.
Finally, in the West South Central (Arkansas, Oklahoma, Louisiana, Texas), the midrange top five were manufactured stone veneers, steel entry door replacements, garage door replacements, basement remodels and roofing replacements. The top five upscale requests were fiber-cement siding replacements, garage door replacements, foam-backed vinyl siding replacements, vinyl window replacements and roofing replacements.
Spring and summer are popular times to look for a new home, but house hunters willing to brave the cold might be rewarded with a great deal. Although you'll have fewer houses to choose from, there are also fewer buyers to contend with.
You may benefit from a discount too as sellers are eager to unload their homes. "Homebuyers can take their time when they're looking for a home because there's less people in the market and there aren't as many bidding wars," says Lisa Foradori, Head of Consumer Direct for Mortgage Originations at Chase.
As you decide whether to hunt for houses during the cold winter months, experts provide advantages and disadvantages to consider.
Fewer buyers frequent open houses during the winter as compared to the spring, when there's more inventory. The benefit is that you'll have some breathing room — hot markets known for bidding wars and multiple offers tend to cool down in the colder months. "It saves buyers money and their sanity," says Mazen Fawaz, CEO of OpenHouse.
Time is money for a seller, and those sellers that open their doors to showings during the holiday season and cold-weather months want their homes to sell. "A seller probably doesn't want to sit with the house through the winter months, particularly if they don't live there anymore and have to make sure the home's being maintained and safe," says Ameer. A buyer may very well benefit from a lower price because the seller doesn't want to continue paying for the expense of the house and would like to get it off their plate.
"Lenders have less inventory in the winter because there are less people buying and selling and we can move the loan through the process faster," says Foradori.
"There's also tax implications for buying before New Year's Eve, which fuels a lot of the activity in the 'off season'," says Fawaz. If you close on a new home before December 31, you can apply the purchase of your home towards deductions for that year, otherwise you'll have to wait a full fiscal year to reap any tax benefits from your purchase.
"When you're searching in the wintertime, you're seeing the home during extreme weather and when the home's systems like heating and plumbing, the roof, doors, windows, and insulation are being tested," says Foradori. You can schedule your inspection during a time when you can see the house's problems. "Depending on what you find, you can probably negotiate the price even further," she adds.
People don't always want their child to change schools midyear for continuity reasons, but this can work in your favor. Depending on your child, a midyear move could give them an opportunity to get acclimated to a new environment before summertime, says Ameer.
"You'll see how the house holds up in harsher weather, but you don't know what the lawn looks like," says Ameer. Getting a sense of the exterior condition can be a challenge when it's dark outside by 4 p.m. If you buy a home, you may not know what the landscaping actually looks like and if the grass needs to be resodded or the condition of the bushes until all the snow has melted in the spring.
Pets, cigarette smoke, pungent foods—they can all make your house smell bad. Queen of Clean, Linda Cobb, gives us her best remedies for getting rid of funky odors and creating a fresh-smelling house.
House just smelling yucky? Cobb creates her own air freshener that's perfect for getting rid of odors in the bathroom, bedroom, or anywhere else in your home. "I buy small decorative spray bottles, fill them with this mixture and place them all over my house," says Cobb. "Vodka is the secret ingredient." It soaks up the smell and evaporates quickly.
Use a hand towel and pour white vinegar over it, then wring it out. "Take the towel and walk through the house swinging it in the air, like you're rooting for your favorite team." This will get rid of the smell in no time, and it's all-natural, so no one is breathing in chemicals," she says.
Doggie or cat smell can be tough to get rid of because it's constant and becomes embedded in fibers. Linda's favorite solution for getting the smell out of the carpet and fabrics: Odorzout. "It's 100 percent natural and animals and kids can be around it without worry," she says. Odorzout is a dry product that you sprinkle on the affected area, and it absorbs the odor. Then you vacuum it up! "A little goes a long way and it works great," says Linda.
Why it works? Douches deal with protein-based smells, and your dog's fur is made up of protein. Works like a charm.
10-20 drops of essential oil such as eucalyptus, citrus, or lavender. (You can find essential oils at the Vitamin Shoppe and your local health food store.
Mix and put into a spray bottle to leave in the bathroom.
If the can itself smells, wash it with warm water and white vinegar. Or try sprinkling in some Odorzout, let it sit a few minutes, then dump out and rinse the can. If the garbage is smelly, but the bag isn't full yet, Cobb suggests dumping in some coffee grounds, which neutralize smells.
So, you're thinking about buying a house. Your first house in fact. Well, we've compiled a few things we think are very important for you to know before jumping in! Here are five home buying tips learned from experience.
There is no such thing as a "turn key".
There will always be things to buy or fix when purchasing a new home. No, you might not have to do a complete renovation job but just bare that in mind when you are looking at houses. Make sure to do your due diligence and look for the odds and end things you might have to purchase right away or fix. For example, shower rods, appliances, outside trash bins, etc. Not everything you see stays with the house – so make sure to ask!
The closing date can and will more than likely change.
Probably one of the most frustrating things in the process of buying a house is when the closing date is changed, especially if your lease has already ended, or if you have already sold your house. So, when purchasing your home, make sure to give yourself some wiggle room. There is no worse feeling than feeling displaced or scrambling to find a place to crash for a few weeks before your home closes.
You will need fluid cash.
Even if you are able to negotiate the seller paying the closing costs and you already have your down payment – there will still be costs throughout the process. For example, things like home inspections, radon tests, appraisals, and terminate inspections all come with a price tag. Make sure you have the fluid cash before jumping in!
Your bank account is your best friend and your worst enemy.
The loan underwriter will go through your finances with a fine tooth comb once you've decided on your home. Seriously, if you deposit money that you found lying around your house more than likely the underwriter will ask for an explanation of where the funds came from and to show evidence of how you got it. To be honest, explaining that I sold a few pieces of furniture on craiglist and deposited it into my account was probably one of the hardest things to prove to my underwriter. So, word of advice, when you start looking at houses (not when you put on offer in) keep track of all of your cash deposits, this will just help you later on.
I hope these tips on buying a house have given you some insight in buying a home! What are some things you think buyers should know before buying a house? Are you ready to buy your first home? What was your experience like? Let us know in the comments below!


Gary feels he's still learning from the example of his lifelong friend, Joseph Dresner. In his role as a Board member, he strives to alter the course of people's lives in significantly meaningful ways that emulate Joseph Dresner's boundless compassion for others.
Gary is singularly focused on seeing the foundation thrive. He draws upon 35 years of experience as a successful business owner, a wide spectrum of professional talents, and considerable personal drive in his work for the foundation. His hope is to ensure that his friend's memory is honored in perpetuity.


Looks BU, but has some broken luster on the high points. Gold toning, very lustrous, someone will surely call this a full BU in the future.
Click the button below to add the 1921 Pilgrim Commemorative Half Dollar, Borderline BU to your wish list.


Begin your day the right way with J.Christopher's or Da Vinci's Donuts for breakfast. J.Christopher's has a killer $10 deal that includes strawberry crepes, French toast, and eggs Benedict, while coffee and donuts from Da Vinci's never disappoints either. Especially maple bacon ones.
Cozy up for lunch or dinner at Crema Espresso Gourmet. This cafe serves more than just coffee, and restaurant week is the perfect time to explore their other just as satisfying menu options.
Craving Italian? You won't be for much longer! For a local and charming mom-and-pop marketplace, we recommend E. 48th Street Market. This family owned and operated store also doubles as a restaurant serving Italian sandwiches, desserts, homemade pastas, and casseroles. Romantics frequent Carbonara Trattoria for its ambiance reminiscent of Italy and its carefully selected menu to match. For a family-style and slightly more filling experience, try Novo Cucina who is known for their pizzas, salads, pastas, and homemade gelato.
Alternatively, you can get creative at Your Pie by building your own 10 inch pizza to go along with your favorite choice of a side. We know it's challenging to decide between all 4 of these restaurant, but that's why we gave you an entire week to work with!
It may be Dunwoody Restaurant Week, but our dining experiences take you far beyond the state line. Pull up a seat and take a culinary tour around the world at these Dunwoody favorites - no passport required!
Find some of Atlanta's best Indian food at Viceroy Royal Indian Dining. If you want a sample of several cultures at once, go to Simha Fusion (formerly known as Com Dunwoody Vietnamese Grill.) They've extended their menu to now include Vietnamese, Indian, and Thai. For a taste of Greece, pay a visit to Grecian Gyro for one of their namesake gyros or a delicious falafel. Add some Spanish flair to your palate with tapas from Eclipse Di Luna, or have a fiesta with authentic Mexican dining at El Azteca in Dunwoody Village! Curious palates seeking the very best from all over should eat at Portico Global Cuisine. Located inside our Le Meridien Atlanta Perimeter hotel, this swanky and eclectic restaurant offers a contemporary twist on worldwide favorite dishes.
Enjoy burgers, sushi, or even both at The Cowfish Sushi Burger Bar for dinner. They're serving up their must-have fusion specialties and bento boxes, alongside complimentary edamame at each table! Save room for dessert because their Mousse is Loose Chocolate Cake is what dreams are made of.
Eat, drink, and be merry at Vino Venue. This restaurant and wine bar is both widely known around Atlanta and a local favorite. With a menu that is just as impressive as their wine selection, Vino Venue is always tough to pass up.
If it's sweets that you seek, you will be happy to see Ali's Cookies or Da Vinci's Donuts on our list of participating restaurants. Both will offer specialty menus allowing their patrons to score sweet deals all week long.
Restaurant Week will have no shortage of steak this year with 3 of Atlanta's finest serving up cuts. It's not every day that you can enjoy a premium steak with all the side fixins for less, so you definitely don't want to pass up a visit to McKendrick's Steak House, The Capital Grille, Fleming's Prime Steakhouse or Fogo de Chao. Our best advice is to pace yourself. With so much to enjoy at such a small cost, you're going to want to savor every juicy bite.
Keep things cool and casual with our bar options for the week. Stop in for lunch at The Bird and take advantage of their beer specials, or watch the game and make new friends at Gilly's - Dunwoody's neighborhood bar that serves the best wings in town.
Eating with a group of people who all have a taste for something different? Go somewhere all-encompassing, with all the shareable items. Enjoy the flavors of summer with a seasonally-inspired menu from Seasons 52, or keep things evergreen at Sage Woodfire Tavern. No objections guaranteed.
New to Dunwoody Restaurant Week this year is Big Dave's Cheeesteaks! The owner is actually from Philly, so you knew these cheesesteaks are the real deal. For just $15, you can have a fully loaded cheesesteak, six wings, fries, AND a drink. Come hungry.
Eating clean? We've got the perfect solution. Diced Foods is another new participant, so you can stay on track while still enjoying the dining deals that restaurant week has to offer. Everybody wins!
Whatever you're in the mood for, Dunwoody Restaurant Week has it. No but really - check out the full list of menus. Morning, noon, or night, we encourage you to experience as many of these restaurants as humanly possible. You'll discover that they each go above and beyond, because that's kind of Dunwoody's thing. As you dine around town June 9-16, be sure to share your food pics with us using #DunwoodyRestaurantWeek or #DineinDunwoody. We'll give you a shout out!


LinkedIn is the best social media platform for engaging with B2B customers both before and after making a sale, according to new research. As studies have shown, the professional network is only growing in importance to marketers, but in the B2B space, it's the most important social network of them all to a lot of businesses.
Is LinkedIn a major component of your marketing and/or sales efforts? Let us know in the comments.
A recent study from Social Media Examiner looked at how marketers are using social media to grow their businesses. It didn't focus exclusively on LinkedIn, but there are a lot signs within it that point to a bigger focus on the network as time goes on.
It's also in third place behind Facebook and Twitter in platforms used by those with less than twelve months experience. As you'd expect, LinkedIn gets more importance placed on it by B2B marketers. Those focused on B2C are placing more emphasis on Facebook, YouTube, Pinterest, and Instagram, while B2B marketers are focused on LinkedIn, Twitter, Google+, and SlideShare (which LinkedIn acquired).
In terms of overall importance to marketers, LinkedIn increased to 21% from 17% since 2014 as Facebook decreased slightly. For the self-employed, LinkedIn scored 23% just behind first-place Facebook (44%).
The study also found that 66% of marketers plan to increase their use of LinkedIn in the future. As you'd guess, B2B marketers are more likely to plan on increasing their use. 80% of B2B marketers plan on doing so versus 56% of B2C marketers. 71% of B2B marketers are interested in learning more about LinkedIn as are 55% of B2C marketers. It's the number two network behind Facebook when it comes to what marketers want to educate themselves about.
18% of marketers are regularly using LinkedIn ads, the study found. That's actually down from 20%. Still, 29% plan to increase their us of LinkedIn ads.
Regalix recently put out its B2B Social Media Marketing 2015 report (via eMarketer) finding that LinkedIn is second only to Twitter as the most used social platform by B2B marketers. Both were significantly ahead of Facebook. All three were well ahead of everything else.
LinkedIn is actually on top of of the list when it comes to channels found to be most effective for customer engagement both before and after sales.
LinkedIn recently extended its ads to publishers sites via LinkedIn Network Display. According to the company, these ads enable marketers to reach a "high-value audience of business professionals" both on and off LinkedIn, while targeting the right people to increase brand awareness with those "who matter most". You can also track the impact of the ads with its campaign and website analytics.
Looking at content types, the Social Media Examiner study found that 69% of marketers plan on increasing their use of blogs. This could further fuel LinkedIn marketing efforts as its publishing platform, which is essentially a blogging platform, is growing at a healthy clip.
The company announced last month that over a million people had published a post using the platform. They first opened it up to everyone in February of last year. The company says over a million unique publishers publish over 130,000 posts a week, and about 45% of readers are in the upper ranks of their industries, such as managers, VPs, and CEOs. The average post now reaches professionals in 21 industries and 9 countries, it says.
Just today, LinkedIn announced that it's expanding the publishing platform globally, opening it up to more languages.
In June, LinkedIn launched a new version of its Pulse news reader app, which makes use of the user's LinkedIn network when determining what content to show them. This should only help with LinkedIn content marketing efforts.
The score is updated on a daily basis regardless of whether or not your'e an actual salesperson.
Are you placing increased emphasis on your LinkedIn marketing efforts? Do you expect to as time goes on? Share your thoughts about marketing on the professional social platform.


RALEIGH, N.C. and HUNTSVILLE, Ala. (May 4, 2017) – NeoNova, an NRTC company, today announced it has acquired API Digital, a leading rural broadband solutions provider. API Digital will continue to operate as a wholly-owned subsidiary of NeoNova while maintaining its headquarters in Huntsville, Ala.
NeoNova and API Digital customers will not experience any immediate changes to their services or billing relationships. The price and terms of the transaction were not announced.
NeoNova's 144 employees nationwide provide help desk, networking and revenue-building services to 230 rural broadband affiliates in 44 states, while API Digital and its 60 employees already serve more than 100 customers across the country.
NeoNova, an NRTC company, helps rural service providers grow by delivering a wide array of subscriber, network management, and professional services leveraged by a powerful service delivery platform and backed by the industry's top professionals. Today, NeoNova serves 230 affiliates, helping them provide exceptional service to more than one million subscribers across 44 states. NeoNova's parent company, NRTC, is a not-for-profit cooperative that provides technology solutions to more than 1,500 electric and telecom members in 48 states to help them bring all the advantages of today's evolving technology to rural America. Visit www.neonova.net, Facebook, LinkedIn, or Google+, and follow @NeoNova_NNS.
Founded in 1999, API Digital is a networking communications and managed IT services provider supporting hundreds of thousands of users across the US and internationally. Leveraging our own network and data centers in cooperation with the world's leading network providers, API Digital delivers carrier-class connectivity and colocation. From our 24x7x365 Network Operations Center, API Digital also provides live support services including proactive monitoring, network engineering, and end-user support.


text:
Dehydrogenase Signal
LDH exists in four distinct enzyme classes.
2000), and top vertebrate predators typically disappear from all
2000), and top vertebrate predators typically disappear from all but the largest habitat fragments (Terborgh et al. 2001). Similarly, Zabel and Tscharnke (1998) found CH5424802 research buy insect predators to be more sensitive to habitat patch isolation than insect herbivores. Among non-rare arthropod species in the present study, there was no evidence that carnivores were more vulnerable to invasive ants than were herbivores or detritivores. Among rare species, however, trophic role was significantly related to vulnerability,
but only for endemic species. Rare endemic carnivores were by far the most likely group to be absent in ant-invaded plots (Table 2), with vulnerable species belonging to six different taxonomic orders. Rare endemic detritivores were the next most vulnerable group. One reason that carnivore species are often at risk is that they tend to exist at lower densities than herbivores and detritivores. But in these
communities, trophic role was most clearly important for rare species, among which population density varied little. Instead, endemic carnivores at our study sites may be especially vulnerable to invasive ants because, in addition to experiencing direct predation and interference competition for feeding or refuge sites, they may also experience exploitation competition for prey resources. Invasive ants are also efficient scavengers, so they may similarly compete with some detritivores or omnivores for food resources (McNatty et al. 2009), although it has also been hypothesized that some detritivores may enjoy an increased resource base consisting of abundant ant carcasses Ispinesib research buy in invaded areas (Porter and Savignano 1990; Cole et al. 1992). Herbivores, as a group, may be least vulnerable SGC-CBP30 mouse because most of them will not be competing with ants for food resources
to any great extent. In addition, some endemic herbivores, such as delphacid planthoppers, are tolerated by ants, perhaps because they produce honeydew (Krushelnycky 2007, Supplementary Tables 2 and 3). Finally, we found no association between body size and the likelihood or magnitude of population reduction as a result of ant invasion, regardless of whether a species was rare or not, or whether we ADAMTS5 controlled for other explanatory factors, including phylogenetic trends. Large body size is often correlated with other factors thought to increase vulnerability in animals, such as lower fecundity, slower development, lower abundance or density and larger range requirements (Reynolds 2003). These associations, however, do not always hold, leading to much variation among taxa in the relationship between size and vulnerability (McKinney 1997; Fisher and Owens 2004). In the present study, larger species had slightly lower densities and tended to occupy higher trophic positions than smaller species, which should make larger species less resilient to losses from ant predation.
The fliC gene appears however not to be useful for distinguishing
The fliC gene appears however not to be useful for distinguishing between R. pickettii and R. insidiosa based on our findings. The division of the groups did not correlate to clinical or environmental association or to their location of isolation. The reasons for the variation learn more between the 16S-23S spacer region and the fliC gene could be potentially due to the structure of the fliC gene. This is demonstrated by Burkholderia flagellin sequences, which exhibit high levels of homology in the conserved terminal regions but differ considerably in the central region [57]. Variation
is a common feature of flagellin proteins, which are believed to fold into a hairpin-like conformation, with the terminal domains being responsible for defining the basic filament structure lying on the inner surface and the central, variable region being surface exposed [58]. In a previous epidemiological study involving sixteen isolates of R. pickettii, eight PS-341 cell line different RAPD profiles were observed for isolates coming from blood culture, distilled water and an aqueous chlorhexidine solution [16]. In another study, involving fourteen isolates of R. pickettii from various biological samples the same RAPD pattern was found in all instances [59], while Pasticci et al., carried out a study involving fifteen isolates of
R. pickettii KU-60019 molecular weight that gave three patterns [27]. The results of our study with a larger number of isolates indicated that there is some diversity in the studied populations but that this is limited and isolates from different environments grouped together. The results obtained with BOX-PCR showed nineteen different profiles among the fifty-nine isolates examined again demonstrating limited diversity (Figure 3b). To the best of our knowledge this is the first reported study of the diversity of R. pickettii and R. insidiosa carried out with BOX-PCR. A similar study carried by Coenye et al., on ninety-seven B. cepacia
Genomovar III isolates Aldol condensation found 20 different patterns with a DI value of 0.821 [60]. The molecular fingerprinting methods used here yielded rapid and reproducible fingerprints for clinical and environmental isolates of R. pickettii and R. insidiosa. Presently, little is known regarding the source of R. pickettii isolates occurring in hospital environments. Investigations by other authors have reported no evidence of patient-to-patient transmission, and they suggest that multiple independent acquisitions from environmental sources could be an important mode of transmission of R. pickettii [5]. The most common sites of contamination were blood-sampling tubes, dialysis machines, nebulizers and other items frequently in contact with water [5]. Conclusions BOX-PCR and RAPD typing was found to be more discriminatory than the typing of genes in R. pickettii such as the fliC gene or the ISR. The majority of isolates were shown to possess similar genotypes by both BOX and RAPD-PCR (Figure 3a, b).
The achromobactin biosynthetic pathway is a particularly valuable
The EPZ5676 supplier achromobactin biosynthetic pathway is a particularly valuable resource for the study of these enzymes as it relies on the action of all three types of synthetase click here [22, 24]. Achromobactin has been shown to be important for virulence in Dickeya dadantii (formerly Erwinia chrysanthemi) [25], and both pyoverdine and achromobactin contribute to epiphytic fitness of P. syringae pv. syringae 22d/93 [21], but the contribution of siderophores
to virulence of P. syringae 1448a has not previously been characterized. We therefore examined the roles of both achromobactin and pyoverdine in virulence of P. syringae 1448a, as well as their relative contribution to iron uptake and growth under more precisely defined conditions. Results Identification Everolimus price and in silico characterization of the P. syringae 1448a pyoverdine locus The biosynthesis of pyoverdine has been most extensively studied in P. aeruginosa PAO1 and most, if not all, of the genes required for pyoverdine synthesis in this strain have now been identified [[6, 10, 26]]. Ravel and Cornelis [8] used the PAO1 pyoverdine genetic locus as a blueprint for annotation of the pyoverdine loci from three other fluorescent pseudomonads, including P. syringae pv. tomato DC3000. We adopted a similar strategy to interrogate
the P. syringae 1448a genome, individually BLASTP searching all of the known PAO1 pyoverdine proteins against the P. syringae 1448a sequence database [27]. The genomic organization of pyoverdine genes in P. syringae 1448a is highly similar to the C1GALT1 P. syringae DC3000 genetic locus presented by Ravel and Cornelis [8], but less similar to that of PAO1 (Figure 1A, Table 1). Given the similarity with the P. syringae DC3000 genetic locus and the excellent earlier analysis of Ravel and Cornelis, we confine our analysis of the non-NRPS genes of P. syringae 1448a to two aspects not previously noted by them. The first concerns the only PAO1 gene that clearly lacks an ortholog in P. syringae, pvdF, which encodes an enzyme required for generating the N5-formyl-N5-hydroxyornithine residues that are present in the PAO1 (but not P. syringae) pyoverdine side chain. Instead,
P. syringae 1448a contains a gene (Pspph1922; marked * in Figure 1A) that is 37% identical at a predicted protein level to the syrP gene of Pseudomonas syringae pv. syringae. Originally mis-annotated as a putative regulatory gene, SyrP has subsequently been shown to be an aspartate hydroxylase that is required for synthesis of the NRPS-derived phytotoxin syringomycin [28]. On this basis we propose that Pspph1922 very likely catalyzes β-hydroxylation of two hydroxyaspartate residues expected to be present in the P. syringae 1448a pyoverdine side chain (Figure 1B), with equivalent iron-chelating roles to the N5-formyl-N5-hydroxyornithine residues of PAO1 pyoverdine. We also note that P. syringae 1448a contains two orthologs of the PAO1 ferripyoverdine receptor gene fpvA.
The distinct genetic divergence and gene organisation patterns of
The distinct genetic divergence and gene organisation patterns of these learn more catabolons suggest disparate evolutionary origins, [12]. In relation to the identification and characterisation of styrene linked
PACoA catabolons, several strain specific traits have been reported in Pseudomonas species studied to date. Comparative analyses of sty gene sequences from Pseudomonas putida CA-3, Pseudomonas fluorescens ST, Pseudomonas species Y2 and Pseudomonas sp VLB120 reveal a high degree of similarity in terms of percentage identity and structural organisation, [1]. However, functional characterisations in P. putida CA-3 and P. fluorescens ST have identified different regulatory profiles in relation to catabolite repression inducing carbon sources and nutrient limitation exposure [6, 7, 13, 14]. With respect to the PACoA catabolon, an essential phenylacetic acid uptake mechanism has previously been characterised in Pseudomonas
putida U, co-ordinately expressed with the catabolic genes [10]. In contrast, a recent proteomic analysis of styrene grown P. putida CA-3 cells indicated that phenylacetic acid transport gene products were not detected in styrene grown CA-3, despite the expression of all other PACoA catabolon proteins [15]. Bioinformatic analysis of PACoA catabolon gene organisation in 102 microbial genomes revealed repeated de novo clustering of the catabolic genes [3]. However, the authors suggested that recombination events and in situ gene replacements by interspecies gene transfer had produced PXD101 considerable diversity in both gene composition and operonic organisation in the pathways. In light of these findings the question arises as to whether the conserved catabolic function of the PACoA catabolon is subject to varied, host dependent regulatory influences in differing species. Elucidation of such host regulatory
influences may identify key flux control points for recombinant strain engineering strategies to optimise biotechnological outputs related to the pathways [9, 16–18]. In this study the Pseudomonas putida CA-3 genome was randomly mutagenised Thymidine kinase with a mini-Tn5 transposon and isolates screened for altered styrene and phenylacetic acid utilisation phenotypes in an effort to identify key regulatory influences acting on these catabolic pathways in this strain. Figure 1 Over view of styrene catabolism. Summary schematic of the major steps in styrene and phenylacetic acid degradation. Gene clusters have been grouped broadly in relation to function, while the arrows reflect common operons observed in Pseudomonads. However, it should be noted that PF-01367338 chemical structure significant variation in PaCoA catabolon gene organisation is seen in nature, such that a standard consensus schematic is not possible.
The developed sensors would be useful at lower phenyl hydrazine c
The developed sensors would be useful at lower phenyl hydrazine concentration [10–14]. By comparing with
reported literature, composite nanorod-based phenyl hydrazine sensor was found to be more sensitive (Table 1). Composite nanorods illustrated drastically elevated sensitivity and lower detection limit as compared to earlier reported phenyl hydrazine sensors [17, 20, 21]. Consequently, the composite nanorods are excellent aspirant for the development of competent and most sensitive phenyl hydrazine sensor. Table 1 Comparison 17-AAG cell line between the sensitivity of composite nanorod sensor and literature Electrode materials Sensitivity (μA.cm−2.μM−1) Reference Composite nanorods 1.5823 Present work Al/ZnO 1.143 [17] Carbon nanotube 0.03 [20] Ferrocene and carbon nanotubes 0.0389 [21] Conclusions selleck kinase inhibitor In summary, composite nanorods were synthesized by a simple and low-temperature hydrothermal process. The detailed morphology of the synthesized composite nanorods
was characterized by XRD, FESEM, FT-IR, XPS, and UV–vis spectra and reveals that the synthesized composite is well-crystalline optically active nanorods selleck chemicals containing Ag, Ag2O3, and ZnO. The synthesized composite nanorods were applied for the detection and quantification of phenyl hydrazine in liquid phase. The performance of the developed phenyl hydrazine sensor was excellent in terms of sensitivity, detection Tryptophan synthase limit, linear dynamic ranges, and response time. Since synthesized composite nanorods have very simple synthetic procedure, low cost, and high sensitivity for phenyl hydrazine sensing, therefore, it is concluded that chemical sensing properties of composite nanorods are of great importance for the application of composite nanorods as a chemical sensor. Acknowledgments The authors would like to acknowledge the support of the Ministry of Higher Education, Kingdom of Saudi Arabia, for this research through a grant (PCSED-014-12) under the Promising Centre for Sensors and
Electronic Devices (PCSED) at Najran University, Kingdom of Saudi Arabia. References 1. Jamal A, Rahman MM, Khan SB, Faisal M, Akhtar K, Rub MA, Asiri AM, Al-Youbi AO: Cobalt doped antimony oxide nano-particles based chemical sensor and photo-catalyst for environmental pollutants. App Surf Sci 2012, 261:52–58.CrossRef 2. Khan SB, Rahman MM, Jang ES, Akhtar K, Han H: Special susceptive aqueous ammonia chemi-sensor: extended applications of novel UV-curable polyurethane-clay nanohybrid. Talanta 2011, 84:1005–1010.CrossRef 3. Faisal M, Khan SB, Rahman MM, Jamal A, Umar A: Ethanol chemi-sensor: evaluation of structural, optical and sensing properties of CuO nanosheets. Mater Lett 2011, 65:1400–1403.CrossRef 4. Jain RK, Kapur M, Labana S, Lal B, Sharma PM, Bhattacharya D, Thakur IS: Microbial diversity: application of microorganisms for the biodegradation of xenobiotics. Curr Sci 2005, 89:101–112. 5.
However, when amino acid sequence alignment
analysis was
analysis was carried out, the putative cadF (-like) ORFs from all 17 C. lari isolates examined in the present study showed amino acid residues of FALG (50% identity) within the amino acid positions 137 – 140, instead of the FRLS residues (Figure 4). No FRLS residues were also detected within any other regions of the cadF (-like) ORF from all 17 C. lari isolates examined. Interestingly, FNLG residues within AdpB (Ad-adhesin in p-Prevotella, B-second identified AR-13324 solubility dmso adhesin) in Prevotella intermedia (a black-pigmented gram-negative anaerobe) [32] was 75% identical to the FALG from C. lari (Figure 4). Therefore, it may be important to clarify if the CadF (-like) protein from C. lari isolates can bind to fibronectin or not. An experiment is now in progress to resolve this. In the present study, for the first time, we have described the cloning, BMS202 sequencing and characterization of full-length Cla_0387 from the 16 C. lari isolates. The CMW values were estimated to be 23,689 – 23,875 Da
for the 16 C. lari isolates and C. lari RM2100 strain and these values were also equivalent to those from two C. jejuni and a C. coli reference strains (Table 2). In addition, the cadF (-like) gene and the Cla_0387 gene may possibly be functional within C. lari isolates, based on the present northern blot hybridization and RT-PCR observations, as shown in Figure 2A and 2B. Thus, the cadF (-like) gene and the Cla_0387 gene could be co-transcribed within C. lari organisms, consisting of an operon.
Since the Cla_0387 showed a high deduced amino acid sequence similarity to the Escherichia coli haloacid dehalogenase-like phosphatase [33], these two may have an important biological relationship within the C. lari cells. In the present study, the authors designed two novel primer pairs (f-/r-cadF1 and f-/r-cadF2) in silico for amplification of an approximate 2.3 kbp region, including the full-length cadF (-like) gene and its adjacent genetic loci, based on sequence Temozolomide information of C. lari RM2100, C. jejuni RM1221 and C. coli RM2228 strains, resulting in successful Tau-protein kinase amplification, TA-cloning and sequencing of those from the 16 C. lari isolates isolated from differencet sources and in several countries. Therefore, the present novel PCR primer pairs would be likely of value for, C. jejuni and C. coli organisms, as well as for other C. lari isolates. A dendrogram showing phylogenetic relationships was constructed by the NJ method [29], based on nucleotide sequence information of full-length cadF (-like) gene from 16 C. lari isolates and C. lari RM2100 and other thermophilic Campylobacter reference strains. As shown in Figure 5, the 17 C. lari isolates form a major cluster separating from the other three thermophilic Campylobacter spp. In addition, the 17 C. lari isolates form some minor clusters, respectively, based on nucleotide sequence information from cadF (-like) gene (Figure 5).
5 fold change threshold) The
5 fold change threshold). The detachment phenotype of nine mutant strains was characterized using visual inspection (recorded with a digital camera), cryosections of 3 h biofilms, and SEM of the surface after draining the tubing. With slight variations, all the mutant strains exhibited detachment phenotypes that were quite similar. Figure 10 presents a panel of results for six of the strains tested. In the top row are mutants exhibiting detachment phenotypes that we consider essentially identical. The detachment phenotypes of the aqy1/aqy1 and MK5108 cost ywp1/ywp1mutants and the orf19.822 double knockout were very PRT062607 manufacturer similar to those shown in the top row.
The macroscopic appearance of the psa2/psa2 mutant was similar to the reference strain but the biofilm was too fragile to withstand the application of the OCT polymer to the surface so cryosections could not be obtained. In the bottom row are detachment phenotypes that exhibited slight variations. Cryosections of the pga13/pga13 mutant did not produce hyphae that were clearly aligned at both edges of the
biofilm. We tentatively attribute this to disruption of the structure during application of the OCT polymer since this biofilm had the appearance selleck screening library of being more fragile than that of the reference strain. In contrast, the mkc1/mkc1 mutant produced a biofilm in which alignment of hyphae appeared to be more pronounced than in the reference strain. (The detachment phenotype of the CAI4 reference strain was the same as the BWP1 reference strain). The detachment phenotype of ACT1-ALS3
biofilm was the only one that differed appreciably from the reference strain in terms of macroscopic appearance. Compared to the reference strain this mutant exhibited fewer regions of detachment that were relatively more displaced from the surface. Figure 10 Detachment phenotypes of selected mutants. All data presented are for 3 h biofilms. The top row of panels in each set are digital camera images (top view, first row; side view, second row). The third row in each set are cryosections and the forth row are SEM images of the surface after draining the tubing. SEM images PAK6 show the most densely colonized regions of the surface that could be found. The biofilm formed from the pga13/pga13 mutant was relatively fragile and this may have contributed to the altered structure of the cryosections. In terms of gross structure the most pronounced differences were seen in the ACT1-ALS3 construct which exhibited fewer regions of detachment that were relatively more displaced from the surface. Discussion Although circumstantial evidence strongly implicates that detachment from C.
Algae 2005,20(3):239–249 CrossRef 27 Kim GH, Klotchkova TA, West
Algae 2005,20(3):239–249.CrossRef 27. Kim GH, Klotchkova TA, West JA: From protoplasm to swarmer: regeneration of protoplasts from disintegrated cells of the multicellular marine green alga Microdictyon umbilicatum (Chlorophyta). J Phycol 2002,38(1):174–183.CrossRef 28. Klotchkova TA, Chah OK, West JA, Kim GH: Cytochemical and ultrastructural studies on protoplast formation from disintegrated cells of the marine alga Chaetomorpha aerea (Chlorophyta). Eur J Phycol 2003,38(3):205–216.CrossRef 29. Kim GH, Klochkova TA, Yoon KS, Song YS, Lee KP: Purification and characterization
of a lectin, Bryohealin, involved AZD0156 in the protplast formation of a marine green alga Bryopsis plumosa (Chlorophyta). J Phycol 2006,42(1):86–95.CrossRef 30. Yoon KS, Lee KP, Klochkova TA, Kim GH: Molecular characterization of the lectin, bryohealin, involved in protoplast regeneration of the marine alga Bryopsis plumosa (Chlorophyta). J Phycol 2008,44(1):103–112.CrossRef 31. Muller WE, Zahn RK, Kurelec B, Lucu C, Muller I, Uhlenbruck G: Lectin, a possible basis for symbiosis between
bacteria and sponges. J Bacteriol 1981,145(1):548–558.PubMed 32. De Hoff P, Brill L, Hirsch A: Plant lectins: the ties that bind in root symbiosis and plant defense. Mol Genet Genomics 2009,282(1):1–15.PubMedCrossRef PI3K inhibitor Authors' contributions JH designed the experiments, analysed the data and wrote the paper. FL maintained the algal cultures. JH and HD performed the experiments. FL, ODC and AW conceived the study and helped to draft the manuscript. All authors read and approved Molecular motor the final manuscript."
The integron includes a site-specific recombination system that integrates and expresses genes present on mobile elements called gene cassettes [1]. The integron platform is defined by three characteristics: an integrase gene (intI) whose product encodes a site-specific integrase, IntI, an attachment site (attI) at which point DNA sequences are inserted and a promoter (Pc) which expresses genes within the gene cassettes inserted at attI [2]. Gene cassettes can be inserted into the integron as individual units but multiple events can lead to large tandem arrays. Integrons are best known for their role in the spread of antibiotic resistance genes in clinical environments [3]. These clinical integrons harbour 1-6 gene cassettes and are often associated with mobile elements such as resistance plasmids and transposons [3]. However, integrons are diverse genetic elements found in approximately 10% of environmental bacteria [2]. In these bacteria, integrons are found in chromosomal locations and rarely carry antibiotic resistance gene cassettes indicating a general role in evolution. Vibrio is a genus of highly adaptable bacteria found in diverse marine-associated learn more niches [4].
(b) M-H curves for the WS2 nanosheets measured at different tempe
(b) M-H curves for the WS2 nanosheets measured at different temperatures, where the diamagnetic signal has been deduced. (c) The FC and ZFC curves for the WS2 nanosheets. Recently, similar ferromagnetic nature was also observed in other layered materials, like graphene, graphene nanoribbons, and MoS2. Matte et al. and Enoki et al. proposed that edge states as well as adsorbed species
affect the magnetic properties of graphene [25, 26]. Zhang et al. prepared MoS2 samples with high density of prismatic edges and showed them to be ferromagnetic at room temperature, where the magnetism arising from nonstoichiometry of the unsaturated Mo and S atoms at the edge [27]. Our previous results indicate that the saturation magnetizations of the exfoliated MoS2 nanosheets increase as the lateral size decreases, revealing the edge-related ferromagnetism [22]. Density functional calculations on inorganic analog of graphite MoS2 reveal that edge
AMN-107 mouse states are magnetic and it appears that magnetism originates at the sulfur-terminated edges due to the splitting of metallic edge states at the Fermi level [28]. Besides, calculation results indicate that only MoS2-triple vacancy created in a single-layer MoS2 can give rise to a net magnetic moment [29]. Shidpour et al. indicated that a vacancy on the S-edge with 50% coverage intensifies the magnetization of the edge of the MoS2 nanoribbon, but such Emricasan manufacturer a vacancy on S-edge with 100% coverage causes this magnetic property to disappear [30]. Furthermore, MoS2 and WS2 clusters (Mo6S12 and W6S12) were shown to be magnetic, where the magnetism arising from the unsaturated central metal atom is due to
partially filled d orbitals [18]. In our case, the WS2 nanosheets with 2 ~ 8 layers thick and the presence of the high density of edges can be seen from the images in Figure 2f. The bends in the layers may arise from the defects. Besides, the high-resolution TEM FER image of the nanosheets shown in Figure 2d reveals a hexagonal arrangement of atoms with zigzag edges. Such defective centers and edges would be associated with the W atoms, which are undercoordinated, resulting in partially filled d orbitals. A high concentration of such edges and defects in our samples could be one of the Gemcitabine possible reasons for the observation of ferromagnetism. Conclusions In summary, even though the observed ferromagnetism in WS2 is in the bulk limit, results indicate that the ferromagnetism for exfoliated WS2 nanosheets persists from 10 K to room temperature. We attribute the existence of ferromagnetism partly to the zigzag edges and the defects in our samples. This unusual room-temperature ferromagnetism, which is an intrinsic feature similar to that observed in carbon-based materials, may open perspectives for spintronic devices in the future. Acknowledgements This work is supported by the National Basic Research Program of China (grant no. 2012CB933101), NSFC (grant nos.
05) (B) Genetic map of genes (open arrows) coding STM3169 within
05). (B) Genetic map of genes (open arrows) coding see more stm3169 within Salmonella-specific JPH203 supplier locus (gray arrows) and genes flanking the locus (closed arrows). Figure 5 STM3169 is a novel virulence protein. Competitive index was determined at 48 h after infection in the spleen (A). Effects of stm3169 disruption on the invasion (B) and the intracellular survival
(C) in the mouse macrophage cell lines, RAW264.7. Cells treated with IFN-γ were infected with S. Typhimurium wild-type and the mutant strains at a multiplicity of infection of 1. At 2 h and 24 h after infection, macrophages were lysed and the bacterial number was determined. Asterisks indicate that differences were statistically significant (P < 0.05). Because it is believed that intracellular Salmonella is likely to be restricted to the acquisition of nutrient substrates from infected host cells, the stringent response could occur in SCV. Thus, we next analyzed the contribution of STM3169 to intracellular survival of S. Typhimurium in macrophages. In accordance with previous data that a ppGpp0 mutant strain deficient in both spoT and relA genes resulted in a severe reduction of intracellular proliferation and suvival [12]. In contrast to the wild-type level of invasion, intracellular survival of TH973 in RAW264.7 cells was reduced, compared with that of the wild-type strain. The reduced CFU of TH937 in IFN-γ
treated-RAW264.7 cells was not more severe than that in the ΔrelAΔspoT double mutant, ΔssaV (SH113, SPI-2 T3SS component-defected mutant), and ΔssrB (YY1, SPI-2 regulator Combretastatin A4 nmr mutant) strain,
but was equal to that in the ΔsseF (TM548, SPI-2 effector mutant) strain (Figure 5B and 5C). These results suggest that the expression of additional virulence factors, like STM3169, in macrophages might be affected in a highly avirulent phenotype of a ppGpp-deficient strain in mice. stm3169 is regulated by the SPI-2 transcriptional regulator ssrB It has been demonstrated that ppGpp mediates the expression of virulence-associated genes involved in bacterial invasion and intracellular growth selleck screening library and survival via global and/or gene-specific transcriptional regulators in S. Typhimurium [12, 14]. Since intracellular growth and suvival of Salmonella in macrophages is dependent upon SPI-2 function, we next confirmed whether expression of stm3169 is regulated by the SsrAB two-component system, which positively controls the expression of SPI-2 genes as well as other genes belonging to the SsrB regulon [32]. To test this, we constructed S. Typhimurium strains carrying stm3169::lacZ transcriptional fusions on the chromosome in the wild-type (SH100) and ΔrelAΔspoT (TM157) genetic background. Salmonella strains carrying the stm3169::lacZ fusion gene (TH1162 and TH1164) were grown in defined MgM medium (pH 5.8) with 0.1% casamino acids and measured β-galactosidase activity. The transcription levels of stm3169::lacZ fusion were significantly decreased in TM157 (Figure 6A).
Ordered Graphic Division Based on Nonsymmetry along with Anti-Packing Design Representation Model.
Evaluation of the pain relievers consequences between Your five mg/kg regarding alfaxalone along with Ten mg/kg involving propofol implemented intravenously throughout kittens and cats.
Higher phylogeographic along with hereditary selection regarding Tidestromia lanuginosa sustains full-glacial refugia regarding arid-adapted vegetation in southeast as well as main Coahuila, The philipines.
Growth and development of Any NEUTRON DOSIMETRY SYSTEM BASED ON Increase SELF-ACTIVATED CSI DETECTORS FOR Healthcare LINAC Situations.
The effect of self-practicing systematic scientific observations in a multi player, immersive, involved personal actuality software vs . bodily products: a randomized controlled tryout.
ampk-signal

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It's November 2018: What SF book are you reading?
I am beginning Thin Air: A Novel by Richard K. Morgan, his much needed return to sci-fi.
Oh nice. Let us know how it is.
I only read The Steel Remains from his dark fantasy series and I loved it. (note to myself, I should really finish that series).
Ofc, I watched Altered Carbon on Netflix like probably everybody else on here and enjoyed that show.
I thought Altered Carbon and the two other Takeshi Kovaks books were among the very best sci-fi that I have read. Hence my excitement for another by Morgan.
I started Gridlinked by Neal Asher. Time to get into the Polity universe.
I know I should be starting with Pardor Moon chronologically but I already own Gridlinked and The Line of Polity so I decided to start there.
I have decided that I am going to reread the Polity series again. My current sci-fi Accidental War isn't taking hold so I'll put it aside for now and I've read so much fantasy lately that I need a change.


text:
Nosotros tres (en alemán Wir Drei ) era un cuadro del artista alemán Philipp Otto Runge, pintado en 1805. Mostraba al pintor junto a su esposa Pauline y a su hermano Daniel. La pintura quedó destruida durante un incendio en 1931, cuando ardió el Palacio de Cristal de Múnich mientras la obra se encontraba allí en préstamo temporal, como parte de una exposición dedicada al romanticismo alemán.

Historia de la obra
En una carta a sus padres escrita el día de Navidad de 1804 el pintor se disculpaba por no poder enviar un regalo y les comunicaba su intención de pintar un cuadro para ellos. Un dibujo preparatorio fechado poco después (7 de febrero de 1805) muestra ya la idea del artista para el cuadro, aunque con algunas variaciones con respecto a lo que fue la obra definitiva. Una vez finalizado, el artista regaló el cuadro a sus padres, tal como había prometido.

La pintura fue propiedad de la familia hasta que en 1892 fue donada a un museo de arte, la Kunsthalle de Hamburgo, donde se exhibió desde entonces y de cuyas colecciones fue parte hasta que en el verano de 1931 fue enviada temporalmente a Múnich para participar en la exposición "Obras de románticos alemanes desde Caspar David Friedrich hasta Moritz von Schwind". La exposición se inauguró el 1 de junio de 1931 en el Palacio de Cristal (Glaspalast) de Múnich, pero el día 6 de junio un incendio provocado destruyó completamente el edificio junto a las 110 obras que formaban parte de la exposición y a las más de 3.000 que formaban la colección permanente de arte del Glaspalast.

Actualmente solo sobreviven fotografías del cuadro anteriores al incendio, además del mencionado dibujo preparatorio, un dibujo de la pintura realizado por uno de los hermanos del artista y una copia de la obra hecha por el pintor Julius von Ehren, que se conserva en la Kunsthalle de Hamburgo.

Nosotros tres fue la primera de las grandes composiciones de retratos que establecieron la fama posterior de Runge y tenía una enorme importancia dentro del conjunto de su relativamente escasa obra, pues había comenzado su carrera artística tardíamente y falleció cuando contaba solamente 33 años. A pesar de estas circunstancias se le considera el más destacado de los pintores románticos alemanes, después de Caspar David Friedrich.

Descripción
Runge es conocido por haber intentado expresar en su obra nociones de la armonía del universo a través del simbolismo del color, la forma y los números, cosa que puede apreciarse en Nosotros tres. Vestido de azul oscuro, el artista, a la derecha del cuadro, mira al espectador. Su cabeza está ligeramente inclinada. Su esposa Pauline, que lleva un vestido amarillo dorado, está a su lado. Tiene un brazo sobre su hombro e inclina la cabeza hacia él. A la izquierda puede verse a Daniel Runge, hermano del pintor, armador y poeta, apoyado en un roble, símbolo de lealtad, protección y estabilidad, que extiende sus hojas protectoras sobre el artista y su esposa. El padre de Runge había dado su consentimiento a la profesión de pintor de su hijo en 1798 por intercesión de Daniel, que se convirtió además en el principal apoyo material del artista durante toda su vida. El hermano, que vivía soltero, era considerado un hombre altamente educado, de mentalidad ideal y con sus propias inclinaciones artísticas. Runge escribió a su hermano a finales de 1802: «Siempre he confiado en ti para lo más alto que hay en mí, y te lo agradezco».

La mano de Pauline, entrelazada con la de su cuñado, actúa como vínculo entre los dos hermanos. La hiedra que trepa al roble simboliza la eternidad de este vínculo. La planta de la esquina inferior derecha parece ser hierba de San Roberto (Geranium robertianum), que en alemán a veces se denomina «Gracia de Dios» (Gottesgnad).

Referencias

Bibliografía
  Frank Büttner: Philipp Otto Runge. Munich 2010

Enlaces externos

Cuadros perdidos
Cuadros de 1805
Cuadros de Philipp Otto Runge
Kunsthalle de Hamburgo

redpajama_set_name: RedPajamaWikipedia


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Books > A Beautiful Day in the Neighborhood
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Listen up homophobes, they are coming for you and your wallet. They told us it was a slippery slope argument, that if we had nothing to worry about if we kowtowed to the gaystapo or the gay brigade.
The Oregon Court of Appeals upheld the ruling that a bakery owner had to pay a $135,000 fine for declining to bake a wedding cake for a lesbian couple. The court ruled that the "emotional damages" done to the couple were so heinous that Melissa and Aaron Klein, owners of Sweet Cakes Bakery, had to pay for their sins to the tune of 6 figures. Ouch. That is probably a significant portion of their income. So much for live and let live, am I right?
But we knew this was their agenda all along. Make the political and social climate so hostile towards anyone who believes in traditional marriage that any dissent will be stamped out accordingly. Everyone else will self-censor out of fear of these people. It's all a master plan at work.
Sounds like Stalinist Russia. Comply or else.
"This is a shocking result which shows the state's relentless campaign to punish Oregonians who live and work according to their faith," said Anna Harmon, a legal representative for the Kleins.
"We believe that freedom of expression for ourselves means freedom of expression for others," said Mike Berry, an attorney for the First Liberty Institute, which took the case on behalf of the Kleins in March.
The Kleins had no choice, but to go out of business shortly after the lawsuit in 2013. Melissa also had to close her online business.
Too bad bigots, you got what was coming to you. You must acquiesce to these people and live by their demands or they will put you out of business in the name of equality and fairness. What's not fair about that?
The next step for the Kleins would be to appeal to the Oregon Supreme Court. They haven't decided on what to do yet, but I doubt the ruling would by any different in hippieville Oregon. But don't worry, nothing will change because of the gay marriage ruling. You have nothing to worry about, nothing to see here folks.


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The Twelve Winds
notes from the wind's twelve quarters
Books Read Lists
Memorable Exchanges
16 August 2008 3 Comments
While at my brother's house, with Sarah, age 4:
Sarah, handing me Donald Duck, says, "Here, play with me. You be the bad guy. The princess [Minnie Mouse] is in trouble and the prince [Mickey] has to save her."
"But what if the prince gets in trouble?" I ask. Sarah frowns at me. She says, "The prince doesn't get in trouble. He's the prince! He saves the princess." Knowing this clearly came from too much Disney influence, I insisted, "But what if he does get in trouble? What then?" I had in mind Prince Philip in Sleeping Beauty, for I would call a dragon Trouble, but I couldn't remember who saved him. My mind races: Aladdin? Jasmine was no help there either.
Sarah replies, "Princes don't get in trouble."
Aha! "Belle saves the Beast in Beauty and the Beast. He almost dies, but she saves him!" I exclaim. Sarah ponders this for a moment. I seize my chance. "So, if the prince gets in trouble, who saves him?"
"The princess!" she shouts, grinning. She gives me a sly look. "…And if the princess is in trouble, who saves her?"
"The prince," I concede, in light of Disney evidence. But I wouldn't relent. "And if they're both in trouble…"
"They save each other!" Good job, Sarah.
Last night while watching the Olympics, I'm knitting, and my dad is listening from the other room. I hear something outside and then in the chimney. I, like anyone who's grown up in a desert, asks in disbelief, "Is it raining?"
"I don't know. Let me check the weather," my dad says, and starts to look it up on his computer. I mute the television and listen… yes, it's raining. But my dad says, "No, we're not supposed to get any rain until Sunday."
"It sounds like it's raining," I comment.
"Oh, well, the radar shows we might have some scattered clouds… but not a lot of rain, and it won't last for very long."
It sounded, however, like it was pouring. "Er, Dad, I think it's coming down."
He finally gets up and opens the door to look out. "Oh!" he exclaims. "It is raining."
"You shouldn't be a meteorologist," I say. He laughs, and holds the door open for Jewely, who comes bounding in yowling because she is wet. Poor cat. How undignified.
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Basic email is available to ND local government as a complimentary extension of the statewide network. See instructions to set up Pioneer Mail in Outlook and instructions to set up iOS (iPhone and iPad). Some agencies are starting to move to Exchange Online as part of the Microsoft Office 365 service offering .
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North Dakota State School Superintendent Kirsten Baesler on Friday announced that Burdell Johnson, of Tuttle, and Sonia Meehl, of Oakes, have been appointed to the state Board of Public School Education. Common Schools Fund provides more than $1 billion for schools over 10 years.
What is North Dakota doing to help low-income students?
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Linnaeus in the Netherlands
April 8, 2019 April 3, 2019 / Maura Flannery
Title page of Carl Linnaeus's Systema Naturae (1735), courtesy of the Biodiversity Heritage Library.
There is a great deal of talk about the European Union these days, and the advantages of open travel among nations. Freedom of movement is a wonderful concept in any age, and it's one experienced by Carl Linnaeus (1707-1778) when he was in his late 20s. Having completed his education in Uppsala, Sweden and having become engaged to a woman whom he very much desired, he set out for three years of study and travel. This wasn't entirely his own idea. His future father-in-law was not thrilled with his daughter's beloved, a physician with few financial resources, so he would only bless the match by having Carl agree to a three-year hiatus. Linnaeus might not have been a man of means, but he was a man who had already learned a great deal about botany and had developed original ideas about how plant diversity should be organized. He also had some experience of travel having spent a few months exploring Lapland, the northern reaches of Scandinavia. So in 1735 he took his manuscripts, packed his bags, and headed to the Netherlands, traveling through Germany on the way. His experiences in Holland and elsewhere in Europe did a great deal to form his ideas and shape his career. This series of posts will look at some of those influences (Blunt, 1971).
It seems that Linnaeus did not make a good first impression on many people. There are a number of stories about men who were put off by his self-possessed manner, and then, as they realized what a good mind lurked behind the bravado, became good friends with him. This was the case with Johannes Burman, a professor of botany and director of the Amsterdam botanic garden. Burman, who was the same age as Linnaeus, had been at the garden for several years working on the Flora of Ceylon, using primarily the herbarium of Paul Hermann, who had collected there in the 1670s. After this brief meeting where Burman was unimpressed by Linnaeus, it would probably have been difficult for either of them to predict that they would be lifelong friends. At this time Linnaeus also visited Albertus Seba who had amassed a large cabinet of curiosities including materials he collected on trips to the East and West Indies. During these years the Netherlands was an important naval power with far-flung mercantile interests, so along with trade goods—like spices and silks, exotic plants, animals, and artifacts also poured into Dutch ports. Even though Seba had sold his original massive collection, he was able to build another and showed some of it to Linnaeus on two visits to his home. He later asked Linnaeus to assist him in preparing a book he was writing on his holdings, but by then the Swede had made other connections (Rutgers, 2008).
Linnaeus next spent two weeks in Harderwijk, the site of a university where for a week's residency he qualified as a doctor, submitting a thesis he had written in Sweden. Then he went to Leiden and showed his manuscript of Systema Naturae to the Dutch botanist Jan Frederik Gronovius, who was so impressed with the work that he arranged for its immediate publication as a thin volume of 14 pages that set out the rudiments of Linnaeus's taxonomic system. Gronovius also gave him a letter of introduction to Herman Boerhaave, who had retired as head of the Leiden Botanic Garden. As with Burman, their relation did not begin smoothly, but eventually Boerhaave appreciated Linnaeus's intelligence and energy. However, none of these meetings landed him a position where he could earn enough money to allow him to remain in the Netherlands. He told them that he would have to return home. That's when Boerhaave offered to fund a trip to Cape Town, South Africa which was then under Dutch control and was proving to be a botanically rich area. Linnaeus, however, after his Lapland expedition, did not much relish a long journey with many probable hardships; Sweden was a safer and easier option.
There are more twists to this story. On his way home, Linnaeus stopped in Amsterdam and again visited Burman, this time with a letter of introduction from Boerhaave. Burman paid more attention to his visitor, especially after Linnaeus was able to identify a rare plant Burman showed him. The latter offered to pay Linnaeus for helping to prepare the Flora of Ceylon, and also convinced him that he should definitely call on George Clifford, a wealthy merchant and horticulturalist who lived near Haarlem. Clifford and Linnaeus got on well because Linnaeus identified many of his hosts' Indian plants and was sorely tempted by Clifford's offer to live and work on his estate, with access to his garden and herbarium. But Linnaeus was committed to Burman. In the end, Burman and Linnaeus visited Clifford, and Burman agreed to free Linnaeus if Clifford would give him a very desirable book displayed in his library: the second volume of Hans Sloane's Natural History of Jamaica. Clifford and Linnaeus were both very fortunate, with the gardener/financier getting an expert to bring order to his collection of specimens, properly name his plants—those in the herbarium and those in the garden—and help in producing a catalogue to make public his botanical treasures. Linnaeus, on the other hand, was freed of economic worries, had a very comfortable place to live, and great resources to work with, including a first-class library. What happens then will be the subject of a later post.
Blunt, W. (1971). The Compleat Naturalist: A Life of Linnaeus. New York, NY: Viking.
Rutgers, J. (2008). Linnaeus in the Netherlands. TijdSchrift Voor Skandinavistiek, 29, 103–116.
Botanists, Botany, Collections, History, Systematics
Carl Linnaeus, Johannes Burman
← Natural History in 17th-Century Britain: Nehemiah Grew
Linnaeus in the Netherlands: Mentors →


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Anyaoku canvasses return to six regions, abolition of states
Ihuoma Chiedozie, Enugu
A former Secretary-General of the Commonwealth, Dr. Emeka Anyaoku, on Tuesday canvassed the abolition of the 36 state governments in the country.
Anyaoku, who spoke at a public symposium organised by the Nigerian Bible Society in Enugu, said most of the state governments are not viable, as they are finding it difficult to pay their workers.
At the event, which was held at the Cathedral Church of the Good Shepherd, Anglican Communion, Enugu, Anyaoku canvassed a return to the regional structure practiced in the First Republic, with the country's six regions forming federating units.
According to him, the current 36-state structure is unwieldy and very expensive.
He explained that the multiplication of administrative organs occasioned by the 36-state structure meant that a major proportion of the country's resources is spent on administration.
He said, "The present governance arrangement we have, with the country comprising of 36 non-viable states, most of which cannot pay the salaries of their teachers and civil servants, is not the best.
"Rather, we should return to an arrangement where the six regions will form six federating units.
"Having 36 states mean we have 36 state assemblies, 36 judiciary services, etc, and all these means that the country is spending too much money on administration," he said.
Anyaoku noted that that Nigeria recorded more development during the era of the six regional governments.
"Those of us who are old enough to remember the six regions know that the country was developing better in those days," Anyaoku said.
Anyaoku commended the Anglican Church in Nigeria for opposing same-sex marriage, despite the acceptance of the practice by the English chapter of the church.
He commended the Bishop of the Anglican Diocese of Enugu, Rt. Rev. Emmanuel Chukwuma, for openly resisting moves by western powers to impose gay marriage on the Nigerian congregation.
Anyaoku said, "One of the problems that is threatening to divide the Anglican Church is the question of homosexuality and same-sex marriage.
"I am not an authority on the Holy Bible but I was brought up to believe that it is not right," he said.
Anyaoku, however, expressed disappointment at the failure of multitudes of religious institutions in the country to address the high rate of immorality and corruption among Nigerians.
He noted that, although Nigeria is widely seen as a highly religious country, majority of Nigerians are of questionable character.
While speaking on 'The role of religion in combating ills in the society', Anyaoku called for a "moral revival" in the country.
Anyaoku said, "It is said that Nigeria is one of the most religious countries but yet there is a high degree of corruption in Nigeria – there are corrupt practices including examination malpractice in Nigeria.
"It is so bad that some parents assist their children to engage in examination malpractice.
"Religious leaders have a role to preach to their followers the revival of the values held dear by our people in the past.
"Children should be taught that success should come from hardwork, they should be taught that the biggest possession a man should have is integrity and good character."
He cautioned religious leaders, including pastors, priests and imams, against accepting donations from "people whose source of wealth is not clear".
Bishop Chukwuma, at the event, delivered a lecture entitled 'Impact of Bible translation on evangelism'.
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