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Brainbow: How to Colorize a Brain
In 1906, the Nobel Prize in Medicine was shared between Camilo Golgi and Santiago Ramón y Cajal. Golgi was largely awarded for creating a type of staining that marked neurons and their ramifications, but this one had the problem that because there were so many neurons it was very difficult to see where each neuron began and ended.
You are here: Home / Neuroscience / Brainbow: How to Colorize a Brain
July 6, 2021 by Pablo Barrecheguren Leave a Comment
Cajal altered the staining to make many fewer neurons distinguishable, which made it possible to see each cell individually and discover that in the communication between neurons there are spaces of separation.
The Brainbow
Thus, from the beginning of histology it has been a constant problem to distinguish neurons and their extensions within a dye. And it is not for less, since our brain contains more than 100,000 kilometers of connections, but fortunately in 2007 was published the first version of a technique that allows us to see individually each one of these connections: the Brainbow.
The concepts of the Brainbow
This technique is based on two very simple concepts:
It is possible to generate transgenic animals that express fluorescent proteins of different colors. For example, there is Green Fluorescent Protein (GFP) or Red Fluorescent Protein (RFP).
If each cell of an animal tissue has different types of these fluorescent proteins and in different amounts, then each cell will have a different color.
From this base, animals were generated (initially worked with mice) that had three or four different fluorescent proteins that were mixed thanks to the activation of a gene (a recombinase also artificially introduced into the genome), which randomly mixes the amounts and types of fluorescent proteins in each cell.
The result is that approximately 100 different color combinations are obtained. Here the key is that this color change is permanent and marks the entire cell, including its ramifications. As this happens in the neurons of the brain, it allows a detailed study of what is known as the connectome, (the set of connections between neurons) and already makes it easier to analyze under the microscope where each neuron connects. However, an important amount of bioinformatics and electron microscopy work is required to handle such a large volume of connections. But the study of the connector deserves it since it is the connections that allow the passage of information between neuronal bodies and are the ones that really explain how the brain works each time it executes a task.
El Brainbow in other organisms
The importance of the Brainbow is such that it has been imported into other organisms where genetic manipulation is possible. An example is Drosophila melanogaster, the fruit fly, where there is intense neuroscientific research both at a basic level studying brain development, and at a biomedical level with works that use Drosophila for research on Parkinson or Alzheimer.
And another example is the Dario rerio or zebrafish, which thanks to the fact that it is transparent during the first phases of its development is used for the study of the initial formation of the nervous system of vertebrates.
Furthermore, it should not be forgotten that although Brainbow was originally a technique developed for neuroscientific studies, it is possible to adapt this tool to other types of tissues.
Brainbow and Cell Biology
With all this, the Brainbow has become one of the most popular tools in cell biology. It still remains an important tool in the study of the connectome, which is undoubtedly the great neuroscientific challenge of the 21st century.
But it can also be used in other tasks such as the study of cellular lineages: once the technique is activated, the change of color in neurons is permanent and also hereditary. In other words, if that cell divides, its daughter cells will maintain the color of its progenitor cell. This makes it possible to study precisely which cells give rise to which tissues, thus helping to understand the field of neuronal stem cells in greater depth.
The Brainbow is not only one of the most eye-catching techniques in molecular biology, it is also one of the most versatile.
Benjamin Richier and Iris Salecker. Versatile genetic paintbrushes: Brainbow technologies. WIREs Developmental Biology (2015). Volume 4.
Dawen Cai, Kimberly B. Cohen, Tuanlian Luo, Jeff W. Lichtman, and Joshua R. Sanes. New tools for the brainbow toolbox. Nat Methods (2013). 10(6): 540–547.
Jean Livet, Tamily A. Weissman, Hyuno Kang, Ryan W. Draft, Ju Lu, Robyn A. Bennis, Joshua R. Sanes & Jeff W. Lichtman. Transgenic strategies for combinatorial expression of fluorescent proteins in thenervous system. Nature (2007). Vol 450.
Michel A. Hofman. Evolution of the human brain: when bigger is better. Frontiers in Neuroanatomy (2014). Volume 8, Article 15.
Zoe T. Cook, Nicole L. Brockway, Zachary J. C. Tobias, Joy Pajarla, Isaac S. Boardman, Helen Ippolito, Sylvia Nkombo Nkoula, and Tamily A. Weissman. Combining near-infrared fluorescence with Brainbow to visualize expression of specific genes within a multicolor context. Molecular Biology of the Cell (2019). Volume 30
If you liked the article about Brainbow by the doctor in Biomedicine Pablo Barrecheguren , you might also be interested in other articles of his:
The remains from a shipwreck: emotional memory and Alzheimer's
Merche Cardona
The remains from a shipwreck. That's what I like to call what Alzheimer´s doesn't take away. And that is, in a way, the representation of what outlasts the disease after such a tremendous tsunami of destruction. After the loss of memories, identity and—why not say it—the very essence of the person,in a person with Alzheimer's there is…
Continue Reading The remains from a shipwreck: emotional memory and Alzheimer's
Cognitive rehabilitation for people with Alzheimer's disease
Dunia Chappotin
Alzheimer's disease is relentless. It progresses steadily while we are waiting for a miracle drug to get us out of this nightmare. While we want to remain optimistic, such magic pills may not reach the market for years to come, and the current medication leaves most people unsatisfied. What should we do? Sit back and do…
Continue Reading Cognitive rehabilitation for people with Alzheimer's disease
Mirror Neuron System: Function, Dysfunction and Rehabilitation Proposals
Anterior cingulate cortex: executive control, emotional control
What is optogenetics?
brainbow, cell biology, connectome, Neuroscience, study of brain connections
About Pablo Barrecheguren
D. in Biomedicine. En Materia, JotDown Spain, Principia, Neurocosas, TED, Big Van Ciencia and Imperial College.
New activity to work on episodic memory with children
The first activity of 2022 of NeuronUP is here! The First Dog is an activity to work on episodic memory with children. What is this activity to … [Read More...] about New activity to work on episodic memory with children
Take a look at the 10 most used NeuronUP activities in 2021
Happy New Year readers! We at NeuronUp enjoy all the year end lists come January! It's fun to look back at the best music, movies, restaurants, bars, … [Read More...] about Take a look at the 10 most used NeuronUP activities in 2021
Neurorehabilitation in brain injury: dual tasks and new technologies.
The performance of neurorehabilitation activities that require the involvement of different processes allows a more comprehensive and realistic … [Read More...] about Neurorehabilitation in brain injury: dual tasks and new technologies.
ADHD Treatment with Neurofeedback
The cognitive process of attention allows us to access the world around us. The brain is concerned with filtering stimuli from the environment, … [Read More...] about ADHD Treatment with Neurofeedback
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Home / American Football / Jared Stegman taking his Aussie style to Germany's Kiel Baltic Hurricanes
Jared Stegman taking his Aussie style to Germany's Kiel Baltic Hurricanes
By Roger Kelly on 03/16/2017@RogerKelly18
The Kiel Baltic Hurricanes looked down under and to the far north to find the quarterback to help lead them hopefully to a German championship. Australian Jared Stegman, quarterback for the Australian national team – Team Outback – and for the past couple of years for Sweden's Uppsala 86ers has landed in Kiel, Germany located on the Baltic Sea coast and is ready to get started.
The versatile Stegman (6'2″, 210 lb), from Brisbane, Australia, gained worldwide recognition at the IFAF World Championships in Canton, Ohio, piloting Australia to a fifth place finish, at times in spectacular fashion. In 2016, he guided the Uppsala 86ers to the Swedish championship game losing in a heartbreaker to the seven time champions Carlstad Crusaders. In 10 regular season games, Stegman threw for 1,988 yards and 28 touchdowns passes.
The Kiel Baltic Hurricanes advanced to the German semifinals in 2016 losing to the powerful Braunschweig New Yorker Lions who went on the win the German title. Kiel finished with an 11-4-1 record.
American Football International caught up with Jared not long after his arrival in Germany.
American Football International: You have had quite a football journey over the past 3-4 years. You were in Sweden for 2 seasons after the 2015 WCs. Why the move to Germany now?
Jared Stegman: The last few years have been great for me. In 2015 I really tried to branch out and experience a new challenge and I took that a step further by becoming the starter in Uppsala in 2016. I feel like I was able to compete and experience some success in the Superserien and all I really want to do is take my passion for the game to the highest level I can take it and the GFL was the next logical step. Fortunately for me, Kiel's HC replied to my message on Europlayers and the rest is history.
AFI: How did you find out about playing in Europe and how did you end up in Uppsala?
Stegman: I can't quite remember how I first heard about playing in Europe. I think one of the Americans who was imported to my local league in Australia had spoken about it. I knew the World Championships were coming up in 2015 in Sweden so my master plan was to gain more playing experience and already be in a European country for the tournament. That's when I google searched all of the teams in the Superserien and found contact details for GM's and Club Presidents. I sent away my bio as well as the all important film and a number of teams got back to me, but Uppsala was the most appealing. Little did I know that the WC was going to be relocated to Ohio and I was too late to change my plan as I'd already signed the contract.
AFI: How much has playing abroad helped you in Australia both with your club team and the Outback?
Stegman: As I alluded to earlier, getting some valuable experience in Uppsala in the lead up to the WC, not only under some great coaches and a veteran QB, but against better competition, helped me perform at a greater level in Ohio. Being able to play for different coaches and with a number of high level, knowledgeable players really affirmed what I knew and believed about the game. It was this reassurance and confidence that I was able to take with me into games for my club and into practice, preparation and general organisation.
AFI: What foods did you enjoy in Sweden?
Stegman: Fika, the coffee and sweet treat tradition, became a part of my day to day life as well as meatballs which were quite cheap and were used in a variety of my meals. I have some fond memories of all you can eat IKEA brunches and MAX hamburgers. On a related note, in the 24 hours I've been in Germany I've had a schnitzel, a pretzel and a bratwurst! I think I'm going to love the food here!
AFI: Did you get a chance to travel much and if so where to?
Stegman: I have been able to do a lot of travel in my two overseas tenures. Coming over and playing football and having a base, it's such a great platform for traveling… I've been lucky enough to get to Finland, Denmark, Iceland, Italy, Greece, Germany and Estonia. Being able to see the world and travel plays a huge part in why I don't settle down into a permanent full time job.
AFI: What are you expecting with Kiel?
Stegman: In terms of I'm expecting a new challenge. I'm expecting to be a part of a complete team and organisation. I'm expecting to carry on the winning tradition.
AFI: What do you think you bring to the team?
Stegman: I think I bring to the team a passion and a gratitude for the game similar to those that I will be playing with. I can relate to my teammates to build a better team because I've been that guy who pays to play, who works full-time, who lifts in between work and practice when that's all the time you have. In terms of on the field, I bring with me the ability for the offense to be versatile and dynamic. I have some moments where I can run the ball and make plays scrambling, but I think my strength will be getting the ball out quickly to our brilliant wide receiver corps. Also, a non-American QB allows for balance on offense as we have a great import receiver and running back.
AFI: What are you looking forward to with this next big move?
Stegman: I'm really looking forward to measuring myself up against, what I've heard is, the best competition in Europe. I want to know what my potential is and by playing at a higher level I really hope it brings out my best.
Interested in playing American football in overseas?
Register on Europlayers.com.
Europlayers is the largest online database of American football players, teams and coaches. It is a connector, bringing together and introducing athletes to overseas teams. On the site you can begin researching teams around the world. Once you create a profile, you become searchable by hundreds of American football clubs around the world.
Roger Kelly is an editor and a writer for AFI. A former PR Director the B.C. Lions of the Canadian Football League for 7 years, he now lives in Sweden writing about and scouting American Football throughout the world.
Related ItemsAmerican FootballAustraliaEuropeGermanyJared StegmanKiel Baltic HurricanesNewsSwedenUppsala 86ers
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View this article online: https://www.claimsjournal.com/news/national/2020/03/20/296125.htm
Anti-Vaccine Ex-MMA Fighter Sues Facebook For Kicking Him Off
A former Mixed Martial Arts fighter sued Facebook Inc. for kicking him off the platform over posts in which he blamed vaccines for his son's death.
Nick Catone says the social network's decision to delete his account violated his free speech and is destroying his business, a gym in New Jersey that he promoted and advertised on Facebook.
Catone's infant son, Nicholas, died in May 2017, according to the lawsuit filed Thursday in federal court in Manhattan. After his son's death, Catone started a daily journal on Facebook, in which he regularly talked about his son and blamed vaccines for contributing to his death.
On Feb. 24, Facebook deleted Catone's account without an explanation, according to the suit. Catone says that as a result, he "has lost all of his memories and pictures he had of his son" on Facebook.
Last year, Facebook announced a policy intended to combat misinformation about vaccines, which health organizations worldwide have determined as safe. As part of the policy, it said it would disable accounts of repeat offenders.
Facebook's censorship of anti-vaccine advocates "embodies its categorical attempt to cater to the pharmaceutical industry," Catone said in the lawsuit.
He is seeking to paid punitive damages "sufficient to punish and deter Facebook for violating the First Amendment."
"Because the sum of $5 billion appears to be insufficient to deter Facebook, the plaintiff asks the jury for a sum significantly in excess of that amount," he said.
Facebook didn't respond to a request for comment.
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Q: How can I deserialize the string in the field when pulling documents with nest in c#? I'm trying to deserialize the string json value in the responseBody field. After get the documents I can deserialize this in the foreach. But I think it would be better if there is a way to do this inside SearchAsync.
var searchResponse = await elasticClient.SearchAsync<TransactionsElasticDto>(s => s
                        .Size(1000)
                        .Query(q => q
                        .DateRange(r => r
                        .Field(f => f.CreatedDate).GreaterThanOrEquals(startDate).LessThan(endDate))
                    ).Scroll("30s"))

The following is an example of a document in elasticsearch
{
        "_index" : "test_transactions_202110",
        "_type" : "_doc",
        "_source" : {
          "consumerId" : 5420,
          "createdDate" : "2021-10-06T11:58:52.2270000",
          "responseBody" : """{"ProgramId":486435,"Test":{"TestId":9728783}}"""
        }
}

redpajama_set_name: RedPajamaStackExchange


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**WARNING: There is a scene in this story which can be a trigger for someone who has suffer from violence and sexual abuse.
REVIEW: My Torin is the first book I read by K. Webster, curiosity got the best of me, the book description drove me in and I was lost once I started reading it.
In a painfully evocative and brutally honest story, K. Webster's My Torin explores these characters's misfortune. In a world where mental illness is almost on epidemic levels, we find stories of perseverance, love and a lot of patience, and it is in stories like My Torin where we perhaps get to experience it first hand without being totally consumed by the struggle of those who suffer from it.
My Torin is the story of 3 very different individuals who are brought together under terrible circumstances. Casey was abandoned as a child, she was left on the doorsteps of a church on a snowy day. Known to the world as Casey Doe or Casey Cocaine as a child she moved from family to family in a system that hardly ever provide the protection children so greatly need.
As part of the system Casey is accustomed to disappointment, to struggle & lack of love, she suffers from ADHD and it is constantly hounded by the thoughts of abandonment & loss. She feels unloved and unwanted but that all changes when she meets Tyler. As she crosses path with this man Casey can't help but feel hope. Tyler is the oldest of the Klines, two brothers who have been hurt by life unpredictability and while they are happy in their own ways he knows that he and his brother need more than each other. That's when Casey comes to the picture.
As the story progresses we are introduced to these characters from different angles. We slowly learn about their likes & dislikes, we are exposed to their differences and soon enough we are captivated by their emotions. While Casey and Tyler are open books, Torin mental illness/disorder keeps him closed up as a window shut by years of paint. In a very emotional and heart-wrenching story, we learn about these characters misgivings, their failures and their desire and need for connection and love. The author does an amazing job bringing these characters together without taking away the essence of their individual struggles.
Torin is a wonderful character, he is kind, passionate and smart. Tyler is caring, understanding and loving and Casey is strong, intelligent and patient. What makes these characters perfect are their flaws, their ability to look beyond pain and while it is hard, they go on with broken hearts letting love and their deep connection map their future.
My Torin is a story of love, connection, struggles, and pain but it is also a story that exposes the reality that so many people with autism experience in life. K. Webster did a fantastic job crafting a story that is perhaps the closest to reality some of us will ever get. This story is beautiful, it is full of hope, love and understanding.


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1120S Sub S Corporate
11834: 1120S - Important Facts
Important Facts About The 1120S
For 2019 calendar year corporate returns, the filing deadline is March 16. For a return, the perfection period does not extend beyond March 26. For an extension, the perfection period does not extend beyond March 21.
Beginning with Drake18, changes to the Schedule B have added 3 new questions. Questions 9 and 10 relate to the business interest expense limitation introduced by the TCJA ( starting on page 64). Question 15 simply asks if the corporation is filing a Form 8996.
Forms 8109 and 8109-B, Federal Tax Coupon, cannot be used to pay S corporation taxes (see 1120S instructions). Make corporate tax deposits electronically.
To pay S corporation tax due, use either the PMT screen or EFTPS (Electronic Federal Tax Payment System).
To pay estimated taxes, file a deposit via EFTPS. The need to make estimated tax payments is only required in certain situations. Thus, the ES worksheet is not required as it is for an 1120 return.
To get more information about EFTPS or to enroll in EFTPS, visit https://www.eftps.gov/eftps/ or call 1-800-555-4477. Additional information about EFTPS is also available in Pub. 966.
To elect for your business to be an 1120S, fill out form 2553 Election by a Small Business Corporation (enter 2553 in the Screen … Code field or click 2553 Election as Small Business Corporation on the Other Forms tab).
Print and complete Form 2553, including signature. The form is not transmitted with the return. Either mail it to the IRS, or scan it for attachment to the 1120S return for e-filing (see Related Links at the bottom of this page).
If an election is to be effective for the tax year you are e-filing, review the filing deadlines in the "When to Make the Election" section of the Instructions for Form 2553. For an election based on a mailed Form 2553, mail it before the return is e-filed and early enough that the election is effective before e-filing occurs - otherwise the return may be rejected.
The maximum number of shareholders is 100. Shareholders can be individuals, estates, exempt organizations described in section 401(a) or 501(c)(3), or certain trusts described in section 1361(c)(2)(A).
Drake Software defaults to a calendar year. If you are creating a fiscal year return, enter the dates on screen 1.
To amend an 1120S, check the amended return box on screen 1. Then go into the return and make the necessary changes.
If you have data entered on the L screen, but the balance sheets are not showing, read the following.
If the corporation's total receipts for the tax year and balance sheets' total assets at the end of the tax year are LESS than $250,000 the Schedules L and M1 are not required, and Question 10 (Question 8 prior to Drake12) of the Schedule B is answered yes.
To force the schedules, in the top left corner of the PRNT screen select Force-print Schedules L and M-1.
Also on the PRNT screen are options to suppress Schedule M-2, the option to print shareholder adjusted basis worksheets, and the option to prepare an extension (which may also be done directly on the 7004 screen). From the PRNT screen you may override particular options, including the envelope coversheet.
If your 1120S return does not update from the prior year, check in the prior year program to see if the return was marked as Final return. If the 1120S return was marked Final return in the prior year, it will not update. If you want to update, go into the prior year return and uncheck the Final return option on screen 1. Then in this year's software go to Last Year Data > Update 20XX to 20XX, enter the EIN and follow the prompts.
To get information to flow to Form 6252 - Installment Sale, enter the information on the 6252 screen in the upper right hand under Current Year and Prior Years columns.
If you receive a PIN error message, check the Title and Signature (name) of the officer signing the return. All fields need to be completed on the PIN screen.
You cannot e-file a bankruptcy return.
Beginning in Drake10, you can e-file an amended return. See FAQ U Amended Return e-Filing in data entry.
To change an 1120 to an 1120S or an 1120S to an 1120, go to Tools > File Maintenance > Change file type. Enter the EIN and select the return type to which you would like to change. Back up the original file before you change the return type. For more information on available file type conversions, see Related Links at the bottom of this page.
Calendar year returns are due on March 15th and the 7004 extends the deadline 6 months, to September 15th. Fiscal year returns are due on the 15th day of the third month after the end of their year. The 7004 extends the deadline 6 months.
For more information, see Form 1120S instructions.
Return Due Dates and Deadlines
1120S - Attaching Form 2553 as a PDF for e-Filing
Changing File Types
1041/1065/1120S - K-1 Codes
1120S - Shareholder Health Insurance
1120S - Calculating Book Income, Schedule M-1 and M-3
QBI Deduction - Frequently Asked Questions
1120S - Entering Officer Information - Form 1125-E
Trial Balance Import for 1120, 1120S, 1065, and 990 Returns
1120, 1120S, 1065, and 990 – Balance Sheet and Auto-balance
Duplicate Business Return Rejection - Not Amended or Superseded


text:
$94K Win for Machinists at Sheppard AFB
September 4, 2019 September 4, 2019 Joe Jackman GOIAM.ORG, iMail, News, Southern
So often we forget – it's the bravest person in the room who stands up in front of their employer and declares their desire to be a union representative. But knowing this fact in no way makes the action easier. Machinist members Guadalupe Anguano and Zacary Allan both quickly realized that when they asked to be part of the IAM team.
In February 2017, a small group working for Solution One Industries (SOI) on Sheppard AFB in Wichita Falls, TX wanted to be represented by the Machinists Union. A few months later, it was time to negotiate their first contract. And that's when it became clear that SOI was not a fan of this idea.
"As soon as negotiations began, the company started to single Guadalupe and Zacary out. Employee writes up appeared overnight, charging these men with everything from not calling in to laying down on the job and even leaving company property unsecured," explained District 776 Business Representative Bud Dulworth. "It was alarming how quickly they were targeted as the lead negotiators on the contract. We knew we had to do something for our union brothers."
The case went to arbitration and struggled through stalls and appeals for more than a year. But in the end, the Machinists Union came out a winner with a ruling that supported the premise that indeed, these two men were fired for their union involvement and both deserved to be made whole again. The IAM ultimately was awarded $94,000 for these union brothers. That money covers back pay, seniority and medical costs. But this is less about the money and more about doing what's just in the face of adversity.
"I am so proud we were able to take care of our union brothers who work at Sheppard AFB and take care of our U.S. Military family there," said IAM Southern Territory General Vice President Rickey Wallace. "These two showed courage and determination in front of a company that held their livelihoods at stake, but yet they didn't back down. That is the purest definition of a trade unionist."
Anguano and Allan are thankful for the money and the union support, but the real winners here are all the people who will benefit from two men who stood up and did what was right when it mattered most.
The post $94K Win for Machinists at Sheppard AFB appeared first on IAMAW .
IAM Family Members Awarded Union Plus Scholarships


The original artwork is painted either on antique ceiling tins or on solid wood door panes from dismantled doors. The paintings hang from strips of leather. The door panels average 22" x 10" and the ceiling tins are 24" x 24".
I keep the old paint on the doors and tins. The subjects are painted in acrylics. I apply combined found materials — rosaries, bottle caps, scrabble letters, beads, old silk flowers, etc., to complete the work. I seal the painting with an acrylic medium.
My desire is to create paintings that are spiritual and inspirational.
Each signed print is encased in a plastic sealed envelope for protection. The prints are shipped in heavy duty cardboard cylinders.
Hello, my name is Sher Chappell.
I studied several years in Paris, France where my name Sher was considered an endearment, so I started signing my paintings "ESSA."
In my opinion, it is important to convey the subject matter to the viewer in a way that reflects love and compassion for others to share.
I live on a working cattle ranch and try to paint every day. My studio is located 3 miles away from the ranch in the Old Lytton Springs High School, which I have refurbished.
Thank you for visiting my site and viewing my artwork!


text:
Secure logging with syslog-ng
Forward integrity and confidentiality of system logs
Track: Security devroom
Room: UA2.114 (Baudoux)
The design, implementation, and configuration of the secure logging service. Its aim is to provide tamper evident logging, i.e., to adequately protect log records of an information system against tampering and to provide a sensor indicating attack attempts. The secure logging service achieves this by authentically encrypting each log record with an individual cryptographic key used only once and protects integrity of the whole log archive by a cipher{based message authentication code. Each attempt to tamper with either an individual log record or the log archive itself will be immediately detected during log archive verification. Therefore, an attacker can no longer tamper with log records without being detected which greatly enhances the use of log archives in forensic investigations.
Log records are normally produced by any information system in order to perform monitoring during normal operations and for troubleshooting in case of technical problems. Log information is equally important for retaining the security of an information system, as security relevant events are recorded and can later be monitored for unusual patterns which may indicate an attack attempt. Examples include log on and log off, startup and shutdown, network service access, network filter rule application, storage access, etc. Log records may also contain valuable information about a system that a potential attacker intends to compromise. If an attacker is able to successfully compromise a system, they are also able to tamper with log records, potentially hiding their traces. This makes forensic analysis extremely difficult, as no reliable data source about system behavior immediately before the attack is available to a security analyst performing incident investigation. Therefore, log information should be appropriately protected. The aim of the secure logging service is to provide tamper evident logging, i.e., to adequately protect log records of an information system and to provide a sensor indicating attack attempts. The secure logging service achieves this by authentically encrypting each log record with an individual cryptographic key used only once and protects integrity of the whole log archive by a cryptographic authentication code. Each attempt to tamper with either an individual log record or the log archive itself will be immediately detected during log archive verification. Therefore, an attacker can no longer tamper with log records without being detected. ost information systems rely on standards in order to provide logging services. One of the most widely adopted standards is the syslog protocol which is specified in RFC 5424. Many implementations of this protocol are available. A popular extensible implementation with additional features is syslog-ng, which is an enhanced logging daemon with advanced features for input and output. Furthermore, it features capabilities for log message filtering, rewriting, and routing. It can be used as a drop-in replacement for existing log daemons on UNIX systems. The implementation of the secure logging service providing tamper evidence and confidentiality of system logs based on the template mechanism of syslog-ng is presented together with an application example.
Stephan Marwedel
Secure Logging with syslog-ng (paper)


text:
Q: C++ Boost::ASIO Thread Pool issue I just created my Thread Pool for game server, but i got one error in compiling what i didn't know how to fix.
Error :

Connection/CConnection.cpp: In lambda function:
  Connection/CConnection.cpp:62:6: error: 'this' was not captured for
  this lambda function

Thread Pool declaration :
class Worker {
public:
    Worker(ThreadPool &s) : pool(s) { }
    void operator()();
private:
    ThreadPool &pool;
};

// the actual thread pool
class ThreadPool {
public:
    ThreadPool(size_t);
    template<class F>
    void enqueue(F f);
    ~ThreadPool();
private:
    // need to keep track of threads so we can join them
    std::vector< std::unique_ptr<boost::thread> > workers;

    // the io_service we are wrapping
    boost::asio::io_service service;
    boost::asio::io_service::work working;
    friend class Worker;
};

template<class F>
void ThreadPool::enqueue(F f)
{
    service.post(f);
}

Function what use it :
void CConnection::handle()
{
     int i = 0;
     ThreadPool pool(4);
     pool.enqueue([i]
    {
     char * databuffer;
     databuffer = new char[16];
     for(int i = 0;i<16;i++)
     {
      databuffer[i] = 0x00;
     }
     databuffer[0] = 16;
     databuffer[4] = 1;
     databuffer[8] = 1;
     databuffer[12] = 1;
     asynchronousSend(databuffer, 16);
    });
}

Can someone tell me where, and what is problem ?

A: My guess is that asynchronousSend is a function in the CConnection class. To call function in object you have to capture this:
pool.enqueue([this] { ... });

As you see I've removed the capture of i as it's not needed, since you declare a local i inside the lambda.