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2. Magnetic resonance imaging...
3. PET and SPECT image guided...
4. Combination of PET and SPECT...
5. The optical imaging guided...
6. Images guided engineered...
7. Conclusions and Perspectives
Theranostics 2012; 2(11):1040-1053. doi:10.7150/thno.4652
Image Guided Biodistribution and Pharmacokinetic Studies of Theranostics
Hong Ding1,2 , Fang Wu2
1. Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL33199.
2. Department of Pharmaceutical Sciences, University at Buffalo, The State University of New York, Buffalo, New York 14260.
Ding H, Wu F. Image Guided Biodistribution and Pharmacokinetic Studies of Theranostics. Theranostics 2012; 2(11):1040-1053. doi:10.7150/thno.4652. Available from http://www.thno.org/v02p1040.htm
Image guided technique is playing an increasingly important role in the investigation of the biodistribution and pharmacokinetics of drugs or drug delivery systems in various diseases, especially cancers. Besides anatomical imaging modalities such as computed tomography (CT), magnetic resonance imaging (MRI), molecular imaging strategy including optical imaging, positron emission tomography (PET) and single-photon emission computed tomography (SPECT) will facilitate the localization and quantization of radioisotope or optical probe labeled nanoparticle delivery systems in the category of theranostics. The quantitative measurement of the bio-distribution and pharmacokinetics of theranostics in the fields of new drug/probe development, diagnosis and treatment process monitoring as well as tracking the brain-blood-barrier (BBB) breaking through by high sensitive imaging method, and the applications of the representative imaging modalities are summarized in this review.
Keywords: Image, biodistribution, pharmacokinetic, theranostics, nanoparticles, antibody.
The properties of biodistribution and pharmacokinetics play a major role in influencing and determining the efficacy and safety for the treatment with a medicine. Currently, several image guided modalities have been applied in biomedicine and even in clinic, including magnetic resonance imaging (MRI), X-ray computed tomography (CT), positron emission tomography (PET), single-photon emission computed tomography (SPECT), electron microscopy, autoradiography, optical imaging and ultrasound (US), etc. They are all noninvasive imaging modalities and proven clinical applications, and some of them are only extended to in-vivo research specimens as small as mice [1, 2]. Among them, PET and optical imaging are regarded as quantitative or semi-quantitative imaging modalities that employ radiotracers or optical tracers to image biodistribution of the labeled drugs or probe loaded delivery systems in the body, meanwhile CT and MRI are normally used for anatomical imaging purposes [3, 4]. In these imaging modalities, non-invasive technique attracted more attention because of its properties of no breaking in the skin and no contact with the mucosa, internal body cavity beyond a natural or artificial body orifice. Non-invasive in vivo molecular imaging can be obtained from PET, MR, CT and visible infrared in vivo optical imaging systems. The discovery of the first modern non-invasive techniques was setup at the end of 19th century based on physical methods: electrocardiography and X-rays. From that time, non-invasive with advantage of penetrating the body rather than a scalpel have continuously enlarged the scope of medical technology. Non-invasive techniques commonly can be used for the purpose of theranostics by diagnostic imaging and therapy on diseases [5-7].
The ability to quantitatively image the biodistribution of therapeutics or drug delivery systems in a noninvasive manner can aid in the development of new theranostic, dose optimization and treatment monitoring. Normally, the information of biodistribution can often be obtained by dissecting the animal, collecting plasma or tissues and being analyzed by high-performance liquid chromatography (HPLC), Enzyme-linked immunosorbent assay (ELISA) etc [8-10], the key advantage of non-invasive imaging is less time consuming and more cost effective of animals and analysis reagents [11]. There is a critical need to establish an effective non-invasive tool to clearly diagnose diseases along with the treatment. If a high effective non-invasive method exists, a cancer patient could be more potentially received effective treatment at an earlier stage with concise targeted drug delivery [12].
Therefore, discussions of perspectives of various imaging guided modalities are focusing on the research applications in biodistribution and pharmacokinetics of delivery systems such as theranostics. The concept of the revolutionary "Theranostic" was originated by Funkhouser in 2002 from one of his reviews. Theranotics is defined as a material that combines the modalities of therapy and diagnostic imaging at the same time within the same dose [13]. It integrates the two features into one "package" to overcome undesirable difference between biodistribution and therapeutic agents. The final goal of the theranostic is to donate materials with the capacity of monitoring the treated tissue, PK and efficacy in the long-term period. This approach will benefit nanomedicine with an effectively style of personalized treatment. In actual applciations, the combination of MRI, CT, PET, or optical imaging and their combination usage may add further impetus to the rapid and high throughput screening of nanoparticle delivery systems based diagnosis and therapeutics (Figure 1). Some representative applications of imaging modalities in the area of biodistribution and pharmacokinetics of nanoparticles for the aim of treatment and diagnosis are also listed in this review.
Illustration of multi-functionalized nanoparticles. Examples of multifunctional nanoparticles for (i) molecular imaging for MRI or PET/SPECT imaging; (ii) drug delivery, (iii) fluorescence detection with a fluorescent probe; (iv) X-ray images such as CT; (v) ultrasonic assistance; (vi) specific targeting, functionalized with specific ligand molecules (e.g. antibody) and (vii) drug payload.
2. Magnetic resonance imaging (MRI) and computed tomography (CT) guided biodistribution and pharmacokinetics study
Magnetic resonance imaging (MRI) is a non-invasive medical imaging to visualize detailed internal structures. For MR imaging, atoms in the body are aligned under a powerful magnetic field. Radio frequency fields change the alignment of the magnetization of atoms. This causes the nuclei to produce a rotating magnetic field detectable by the scanner, which can be recorded to construct an image of the body. Strong magnetic field gradients cause nuclei at different locations to rotate at different speeds, thus providing 3D spatial information. Comparing with other medical imaging techniques such as computed tomography (CT), MRI provides good contrast between the different soft tissues of the body, which make it especially useful in imaging brain, muscles, the heart, and cancers [14, 15]. Another advantage of MR imaging is that it does not need to use ionizing radiation which has damage to cell structure once be exposed.
X-Rays computed tomography, also called computed tomography (CT scan) or computed axial tomography (CAT scan), can make use of radiation to get an internal view of the body for medical imaging employing tomography created by computer processing. Although CT technique is regarded as birth defects or DNA altering because of powerful X-ray radiation exposure, it is still largely employed in hospitals, especially used for broken bones and skulls. Not like MRI imaging which can provide subtle differences between the different kinds of soft tissues, CT can demonstrate the difference between bone density and soft tissue. Although MRI and X-ray are both imaging techniques for organs of the body, the difference in application is that MRI images provide a 3D representation of organs, which X-Rays usually cannot.
Dr. Sgouros and colleagues applied MRI based preclinical biodistribution and localization data and the therapeutic potential of trastuzumab monoclonal antibody (mAb) (anti-HER2/neu) against ovarian carcinoma. By MRI images, they found out the rapid blood-pool uptake (5-9 hours) of mAb after intraperitoneal injection followed by tumor localization (26-32 hours). And micro-PET finding was consistent with the data from high resolution MRI images slices for mAb bio-distribution in main organs of tumor-beard mice [16]. Dr. Teng et al conjugated anti-mouse OxLDL polyclonal antibody to polyethylene glycol-coated ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles, and imaged at 7.0 Tesla MRI on a carotid perivascular collar model in atherosclerotic disease mice. The results showed MRI signal loss in the carotid atherosclerotic lesions after administration of targeted anti-OxLDL-USPIO at 8th hour and 24th hour, which means that anti-mouse OxLDL antibody conjugated magnetic nanoparticle can be used as a strategy for the therapeutic evaluation of atherosclerotic plaques in vivo by MRI imaging [17].
CT became an important tool in medical imaging to supplement X-rays and medical ultrasonography since 1970s. It has more recently been used for preventive medicine or screening for disease. CT is regarded as a moderate- to high-radiation diagnostic technique. The radiation dose for a particular study depends on multiple factors: volume scanned, patient build, number and type of scan sequences, and desired resolution and image quality. Normally, the high resolution and image quality means the high exposure of radiation to patients. The high ionizing radiation in CT application can damage the DNA double strand structure in human body. In one study, researchers found out at a typical CT scanning, 40%-100% of the irradiated cells is damaged by one or more double strand breaks in DNA molecule. Although there is repair process in cells for damaged and broken DNA, the results are not satisfied [18]. That's why by now, CT technique is designed to combine with other image techniques for better application in patients.
3. PET and SPECT image guided biodistribution and pharmacokinetics study of theranostics
3.1 Radiolabeling of antibody therapeutics and principle of PET imaging
PET imaging is another kind of non-invasive option for diagnosis. Since biodistribution of antibody-based therapeutics is a key consideration that can be modulated to impact the ensuing in vivo pharmacological effect. After being labeled with radionuclide of sufficient long half-life, antibody therapeutics can be monitored using PET (positron emission tomography) imaging with respect to pharmacokinetics and its biodistribution. Normally, it is assumed that the labeling method does not perturb the property of the molecules with respect to metabolism, specific binding or non-specific interactions. The labeling isotopes include positron emitters such as 11C, 15O, 18F, 68Ga, 64Cu, or 76Br with half-lives of 20 minutes, 2 minutes, 2 hours, 68 minutes, 13 hours, and 16 hours respectively. Long-lived positron emitters such as 89Zr and 124I with half-lives of several days, 3.2 days for 89Zr and 4.2 days for 124I, are also available [19]. Among them, Copper-64 (64Cu) is a promising PET radionuclide, particularly for antibody-targeted imaging [20]. The reported methods for modification include addition of labeled groups or radiolabel chelating groups. Compared with radio-halogenation, radiometal ion complexation reactions have simpler chemistry and allow tracer production kits. For example, chelator 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid (DOTA) is commonly used in radiolabeling copper radioisotope with antibody [21].
'MicroPET' is a small-animal positron emission tomography which uses pharmacologically or biochemically active compounds labeled with short-lived or long-lived positron-emitting radionuclides to monitor their biodistribution, physiological processes and biochemical pathways etc [22]. These radionuclides are incorporated into molecules during the radiosynthesis step, generating the radiotracer. PET scans are acquired following the injection of the radiotracer. The radiotracer accumulates in the tissues, and its radionuclide decays by emission of a positron (anti-electron). After travelling at most a few millimeters, a positron will collide with an electron, simultaneously releasing two gamma rays (photons) with energy of 511 keV into opposite directions. These two photons are detected by the PET camera and by collecting a statistically significant number of radioactive events, mathematical algorithms reconstruct a three-dimensional image that shows the distribution of the positron-emitting molecules in the tissues [19]. Single-photon emission computed tomography (SPECT) is also one kind of nuclear medicine tomographic imaging technique using gamma rays which can provide 3D information as PET as well. Normally, a marker radioisotope could be attached to an nanoparticles or antibody. After being administered in vivo, the gamma-emission of the isotope from the radiolabeled materials allows the drug to be seen by a gamma-camera. SPECT can generate a true 3D image, which permit accurate localization of organs. Therefore, it can be used to provide information about localized function in internal organs, such as functional cardiac or brain imaging [11].
3.2 PET images guided biodistribution and pharmacokinetic study of theranostics
Targeted therapeutic and diagnostic nanocarriers functionalized with antibodies, peptides or other targeting ligands that recognize over-expressed receptors or antigens on tumor cells have potential in the diagnosis and therapy of cancer. Biodistribution study of therapeutic antibodies by PET methodology could quantitatively measured by radiotracer in organs/tissues. Biodistribution and pharmacokinetics could also be recorded by blood sampling at predetermined time points, preferably associated with radiometabolite evaluation with respect to time [23]. PET images were used from heart to determine blood concentrations. Briefly, nude mice bearing human colon tumors (LS-174T) were injected intravenously with a chimeric 124I anti-CEA mAb (cT84.66) and imaged serially 1 hour to 7 days postinjection. The results showed that PET blood curves agreed well with direct measurements within 12% at all time points. The 4.2-day half-life of 124I favors its use for PET study of monoclonal antibodies (mAbs). Therefore, quantitative biodistribution measurements could be achieved from tumor-bearing mice by using 124I anti-CEA mAbs with standard microPET acquisition and processing techniques. Although from the ROI of heart on the PET images, the blood concentrations can be calculated, most of the case, the pharmacokinetic study was done by withdrawing blood sample and analyzing them with radioassay or ELISA [24].
The distribution of monoclonal antibodies is determined by the rate of extravasation in tissue, the rate of distribution within tissue, binding affinity in tissue and the rates of elimination from tissue, route of injection, species etc. Although PET imaging has been applied in the study of Integrin αVβ3 expression or peptide antagonist [25-27], the mAbs are large molecules with MW of about 150 kDa, it seems to be very slow for them to diffuse across vascular endothelial cells. The movement of antibody into tissue by convective transport is thought to be the primary mechanism for the distribution in tissue [28]. The transport is mainly determined by the movement rate of fluid from blood to tissue and by the sieving effect of paracellular pores in the vascular endothelium. Normally, the biodistribution of drugs requires sacrificing a number of rodents and quantitating the tissue concentration by HPLC, ELISA or by counting the radioactivity emitting from the tissue. MicroPET imaging provides a non-invasive way of quantitation of the concentration of mAbs in tissues, especially useful for monitoring the kinetics in target organ such as tumor.
For instance, a chimeric anti-CD105 monoclonal antibody, TRC105, was conjugated to 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and labeled with (64)Cu. Serial PET imaging revealed that the 4T1 tumor uptake of the tracer was 8.0 ± 0.5, 10.4 ± 2.8, and 9.7 ± 1.8%ID/g (percentage of injected dose per gram tissue) at 4, 24, and 48 h post-injection, respectively, higher than most organs at late time points which provided excellent tumor contrast. Biodistribution data as measured by gamma counting were consistent with the PET findings [11]. Non-invasive PET imaging of vascular endothelial growth factor (VEGF) expression using radiolabeled bevacizumab has been investigated in ovarian cancer models [29]. Small-animal PET and microCT images were used to calculate tumor uptake and compared with ex vivo biodistribution at 168 h after injection. The tumor accumulation of 89Zr labeled bevacizumab was calculated to be 7.38 %ID/g using small-animal PET imaging, which is equivalent to ex vivo biodistribution studies. Liver and spleen were the other organs that uptake tracer bevacizumab. This project demonstrated the quantization of radio-labeled bevacizumab in tumor using non-invasive imaging is convenient, which was also validated by ex vivo biodistribution studies. Since VEGF is a major angiogenic factor responsible for the development of tumor vasculature, the expression level of VEGF could be regarded as a factor indicating disease progress. PET imaging were performed in mice bearing human colorectal cancer (HT29) xenografts to investigate the in vivo biodistribution of VEGF after injection of 64Cu-DOTA-bevacizumab. The accumulation of 64Cu-DOTA -bevacizumab in the tumor was 22.7 %ID/g, 24 %ID/g, 19.0 %ID/g at 24, 48 and 72 h, respectively, which was significantly correlated with VEGF expression as measured by western blot (q = 0.81, P = 0.004) [30].
Currently, the PK and biodistribution were also conducted not only for antibodies, but also for antibody or peptide functionalized theranostics. For example, Dr. Cai W etc. employed PET imaging using (66)Ga as the radiolabel to quantitatively evaluate the pharmacokinetics and tumor targeting efficacy of nano-graphene. Nano-graphene oxide (GO) sheets with covalently linked, amino group-terminated six-arm branched polyethylene glycol (PEG; 10 kDa) chains were conjugated to NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid, for (66)Ga-labeling) and TRC105 (an antibody that binds to CD105). TRC105-conjugated GO was specific for CD105 in cell culture. (66)Ga-NOTA-GO-TRC105 and (66)Ga-NOTA-GO exhibited excellent stability in complete mouse serum. In 4T1 tumor-bearing mice, these GO conjugates were primarily cleared through the hepatobiliary pathway. (66)Ga-NOTA-GO-TRC105 accumulated quickly in the 4T1 tumors and tumor uptake remained stable over time (3.8 ± 0.4, 4.5 ± 0.4, 5.8 ± 0.3, and 4.5 ± 0.4 %ID/g at 0.5, 3, 7, and 24 h post-injection respectively; n = 4). PET imaging successful demonstrated the in vivo tumor targeting property with GO, showing them to be suitable nanoplatforms for future biomedical research such as cancer theranostics [31].
Andresen TL et al covalently attached octreotate (TATE) to the distal end of DSPE-PEG(2000) on PEGylated liposomes with an encapsulated positron emitter (64)Cu that can be utilized for PET imaging. TATE are somatostatin peptide analogs, can target Somatostatin receptors (SSTRs) that are over-expressed in neuroendocrine tumors (NETs). They used PET imaging to investigate the biodistribution and pharmacokinetics of the (64)Cu-loaded PEGylated liposomes with and without TATE. They found that the presence of TATE on the liposomes resulted in a significantly faster initial blood clearance in comparison to control-liposomes without TATE. PEGylated liposomes with or without TATE accumulated at significantly higher quantities in NETs (5.1±0.3 and 5.8±0.2 %ID/g, respectively) than the free peptide (64)Cu-DOTA-TATE (1.4±0.3 %ID/g) 24h post-injection. Importantly, (64)Cu-loaded PEGylated liposomes with TATE showed significantly higher tumor-to-muscle (T/M) ratio (12.7±1.0) than the control-liposomes without TATE (8.9±0.9) and the (64)Cu-DOTA-TATE free peptide (7.2±0.3). The higher T/M ratio of the PEGylated liposomes with TATE suggests some advantage of active targeting of NETs, although no absolute benefit in tumor accumulation over the non-targeted liposomes was observed. The results showed that (64)Cu-loaded PEGylated liposomes with TATE conjugated to the surface could be promising new imaging agents for visualizing tumor tissue and especially NETs [32].
3.3. SPECT images guided biodistribution and pharmacokinetics study of theranostics
Single-photon emission computed tomography (SPECT) is a nuclear medicine tomographic imaging technique using gamma rays. Normally, a marker radioisotope could be attached to nanoparticles or antibody. After being administered in vivo, the gamma-emission of the isotope from the radiolabeled nanoparticles or antibody allows the drug to be seen by a gamma-camera.
Since the source of SPECT images are gamma ray emissions, the radioisotope decays and emits gamma rays, which can be detected by a gamma camera to obtain 3D images, which permit accurate localization of organs [33]. This information is typically presented as cross-sectional slices through the patient, but can be freely reformatted or manipulated as required. Therefore, it can be used to provide information about localized function in internal organs, such as functional cardiac or brain imaging. For example, 3D acquisition SPECT of the liver was performed to investigate the correlation between tumor accumulation of In-111-bevacizumab and VEGF-A expression in patients with colorectal liver metastases [34].
Although heart has been used as an organ in PET imaging to estimate blood concentration, for pharmacokinetic study using SPECT imaging, the commonly used method is to collect blood samples from patients or animals at timed intervals after injection of the radioisotope-labeled mAb [23]. The major advantages of radionuclide-based molecular imaging techniques (SPECT and PET) over other modalities (e.g. optical and MRI) are that they are very sensitive (down to the picomolar level), quantitative, and there is no tissue penetration limit. However, one disadvantage is that the resolution (typically >1 mm) of either SPECT or PET is not as high as the other imaging modalities such as MRI. In most cases, the purpose of labeling the nanoparticle with a radionuclide was for the non-invasive evaluation of its biodistribution, pharmacokinetic properties, and/or tumor-targeting efficacy with SPECT or PET [35, 36]. Radiolabeling of nanoparticles was also applied for PK and biodistribution studies for the purpose of theranostics. Dr. Helbok et al radiolabeled DTPA-derivatized lipid-based nanoparticles including including (111)In and (99m)Tc for SPECT and PET images with therapy in vitro and in vivo. Their study showed that PEGylated DTPA-nanoparticles were suitable for targeting applications. Biodistribution in Lewis rats revealed no significant differences between nanoparticles in terms of DTPA loading and particle composition; however, different uptake patterns were found between the radionuclides used [37]. Dr. DeNardo et al applied 111In-labeled, chimeric L6 (ChL6) monoclonal antibody linked iron oxide (IO) nanoparticles for studies on pharmacokinetics, tumor uptake, and therapeutic efficacy in athymic mice bearing human breast cancer tumors. The 111In-labeled ChL6 was conjugated with carboxylated polyethylene glycol (PEG) on dextran-coated iron oxide nanoparticles with one to two ChL6 antibodies per nanoparticle (Fig 2). Inductively heating the nanoparticle by externally applied alternating magnetic field caused tumor necrosis after 24 h of therapy. After nanoparticle injection, SPECT imaging was carried out to quantify the nanoparticle uptake in the tumor, which was about 14 percentage injected dose per gram (%ID/g) at 48 h post-injection. A delay in tumor growth occurred after the alternating magnetic field treatment, which was statistically significant when compared with the untreated group [38, 39]. Dr. Misri and colleagues also applied SPECT imaging for mesothelin expressing in tumors by evaluation of 111In labeled antibodies. Their findings indicated that the antimesothelin antibody mAbMB may be developed into a diagnostic agent for imaging mesothelin-expressing cancers [40]. Dr. Wu et al also successfully applied gamma scintigraphy by pulsincap capsule in the gastrointestinal tract of dogs by to find out the desintegration and transit behavior of Tetramethylpyrazine phosphate. Their SPECT images confirmed the time controlled capsule releasing in the gastrointestinal delivery in dog at the expected time schedule [41].
Simple radioimmuno-nanoparticles diagram of a BNF-type particle coated with PEG to link 111In-ChL6. The particle core has ferromagnetic property. Adapted from [39].
4. Combination of PET and SPECT with CT, MR or NIRF images guided biodistribution and pharmacokinetic studies of theranostics
Imaging modalities such as CT and PET have been applied sequentially in the diagnosis and staging of disease and in monitoring the effects of therapy for several years. Although PET offers high sensitivity in terms of molecular concentrations and it is possible to measure picomolar amounts of a PET radiopharmaceutical in a tissue. With a three dimentional images obtained by PET, establishing a ROI (region of interest) and set the location of the organs or tissues in the images is of critical importance. Since accurate anatomic localization of functional abnormalities seen on PET scans is well known to be challenging because of the lack of detailed, high-resolution anatomy, the fusion of PET with CT in one camera unit (PET/CT) or fusion of PET image with MR images provides a merged image where not only the physiological status but also the anatomical details may be seen on the same image, which opens new ways of diagnostics and quantitation [42-46]. Indeed, in many cases anatomic imaging is used exclusively, although functional imaging with PET is fulfilling an increasingly important role in the staging and therapy monitoring processes, particularly when the CT scan is equivocal. This situation changed dramatically with the recent introduction of the combined PET/CT scanner, an approach that solves the fusion problem through hardware rather than software. This device provides a medical imaging department with the capability to acquire accurately aligned anatomic and functional images for a patient from a single scanning session [47]. In another word, anatomical/functional imaging techniques help to identify the exact location of the signals. Since the patient remains positioned on the same bed for both imaging modalities, temporal and spatial differences between the two sets of images are minimized.
Schneider DW et al used whole-body small-animal PET to compare the bisodistribution and tumor localization of 86Y or 111In-labeled antibody formats, derived from a single antimindin/RG-1 mAb in a prostate tumor model. The images were colocalized with simultaneous micro-CT imaging. The antibody accumulation in the tumor correlated with molecular size. The larger antibody formats (IgG and miniantibody) gave higher tumor uptake levels than did the smaller formats (diabody and scFv). These larger formats may be more suitable for radioimmunotherapy applications. The smaller formats were rapidly cleared from circulation, and the diabody, which accumulated in the tumor, may be more suitable for radiodiagnostic applications. The combination of PET with CT provides easier localization of tissues [34, 48].
PET is sensitive in the process of detecting and quantifying a low abundance molecular target in cells while MRI is capable of providing exquisite anatomy for organs. Thus, an integrated PET/MRI system will permit the simultaneous acquisition of several parameters in highly accurate spatial and temporal registration. Quantitative values from PET of a large number of biological parameters are complemented by the high-resolution information provided by MRI (in the micromolar range) to yield complementary information. An MRI-based method has the potential to improve PET image quality, increasing its reliability, reproducibility, and quantitative accuracy [49]. PET data and MRI data have been combined for monitoring the drug anticancer effect [50], the response of patients to antibody treatment [51] as well as for detection of lymph node metastases [52], which is very important for the improvement of tumor detection and for the guide of mAb-based therapy.
Overall, accurate quantization of PET tracer uptake levels in tumors is important for staging and monitoring response to treatment. The combination with anatomical information obtained from CT will improve quantization of tumor values in terms of bias and variance [53]. Using appropriate software to fuse PET and CT image together and accurately quantify the tumor uptake level of tracer is of critical value. There is PET/CT scanner available for clinical study for the [54-56]. However, the integrated equipment which combines microPET and contrast-enhanced microCT for small animal study is still at the starting beginning stage [57, 58].
PET and NIRF imaging was also combined to study the ex vivo biodistribution 64Cu-DOTA-NuB2-Alexa Fluor 750 in CD20 positive Raji lymphoma-bearing mice. Raji tumor showed significantly higher uptake of DOTA-NuB2-Alexa Fluor 750 than that of DOTA-Alexa Fluor 750 (p<0.05). Significant correlation was obtained between the organ-to-muscle ratios measured by the radioactivity and fluorescence intensity [59]. In Xie's work, iron oxide nanoparticles with triple functional of PET/NIRF/MRI were applied for the in vivo biodistribution study. To assess the biophysical characteristics of this novel nanosystem, the HSA coated IONPs (HSA-IONPs) were dually labeled with 64Cu-DOTA and Cy5.5, and tested in a subcutaneous U87MG xenograft mouse model. In vivo PET/near-infrared fluorescence (NIRF)/MRI tri-modality imaging, and ex vivo analyses and histological examinations were carefully conducted to investigate the in vivo behavior of the nanostructures. This nanosystem is well-suited for dual encapsulation of IONPs and drug molecules, with the compact HSA coating, the HSA-IONPs manifested a prolonged circulation half-life; more impressively, they showed massive accumulation in lesions, high extravasation rate, and low uptake of the particles by macrophages at the tumor area [60].
SPECT has been combined with MRI to investigate the pharmacokinetics and biodistribution. For example, 99mTc-labeled superparamagnetic iron oxide nanoparticles were employed for multimodality SPECT/MRI of sentinel lymph nodes. In Dr. Madru's work, Nanoparticles with a solid iron oxide core and a polyethylene glycol coating were labeled with (99m)Tc. Wistar rats was injected subcutaneously and sacrificed 4 h after injection. Animals were imaged with SPECT/MRI and the microdistribution within the lymph nodes was studied with digital autoradiography. The result showed that the accumulation of (99m)Tc-labeled superparamagnetic iron oxide nanoparticles (as the percentage injected dose/g [%ID/g]) in the lymph nodes was 100 %ID/g, whereas in the liver and spleen it was less than 2 %ID/g. Digital autoradiography images revealed a nonhomogeneous distribution of (99m)Tc- labeled superparamagnetic iron oxide nanoparticles within the lymph nodes; nanoparticles were found in the cortical, subcapsular, and medullary sinuses [61].
5. The optical imaging guided biodistribution and pharmacokinetics study of theranostics
Optical imaging is a photonic technique which applied in the area of imaging, describing the behavior of visible, ultraviolet, and infrared light used in imaging [62, 63]. These optical techniques, such as bioluminescence and fluorescence, are emerging as powerful new modalities for molecular imaging in disease diagnosis and therapy. Optical imaging offers higher sensitivity and temporal resolution than PET at the same spatial resolution in small animals, but unlike PET is limited to a few centimeters of tissue depth [35]. The bioluminescence and fluorescence can be obtained from dyes, specific metal materials, and biological resources etc and already used in diagnosis and therapy in research and clinic [64-66]. With the help of combing of innovative molecular biology and chemistry, scientists have developed optical methods for imaging a variety of cellular and molecular processes in vivo, including the receptor-ligand interaction, tumor targeting, bio-distribution of proteins such as antibody, etc. Whereas optical imaging has been used primarily for research in small-animal models, in some specific area optical imaging may have the possibility to translate into clinical medicine. The advantages of optical imaging for assessing antibody pharmacokinetics in vivo are the conjugation chemistry, cost, and high-speed throughput. The fluorophore conjugation chemistry is often carried out with a commercially available kit and can be performed in most laboratories without special license, equipment, hazardous precautions, or specific training. The detection systems are generally 30 to 50% as expensive as those for small-animal SPECT or PET. Planar fluorescence images are generated within seconds or minutes, typically with a photograph overlay. Optical imaging experiments also can accommodate four to five animals per scan, allowing an efficient researcher to perform 100+ animal images per hour, whereas PET and SPECT imaging is often limited to one to two animals per 10 to 30 min scan. Such throughput and cost combined with expected improvements in quantification from advances in instrumentation provide an exciting outlook for this preclinical fluorescence imaging approach [67].
A key challenge for pharmacists and clinic doctors is defining the disposition of drugs such as monoclonal antibody and pharmacokinetic property in whole animals or patients. Ongoing discoveries of these potential target molecules in disease also drive pharmaceutical and biotechnology companies to find leading agents that selectively regulate these molecular pathways in vitro and in vivo. Among all the existing techniques, optical imaging including bioluminescence and fluorescence, are emerging as key techniques to meet these challenges and advance molecular imaging in preclinical research and patient care. Bioluminescence is the process of light emission in living organisms, it is a chemical process wherein light is emitted during the interaction of a protein produced in engineered cells and an administered substrate, for example firefly luciferase and luciferin [68]. Bioluminescence imaging is a technology developed over the past decade that allows for the noninvasive study of ongo-ing biological processes in small laboratory animals. Bioluminescence imaging utilizes native light emission from one of several organisms which contain bioluminescence. Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation with a different wavelength. It is a form of luminescence. Fluorescence imaging uses probes that emit light (fluorescence) after the excitation at a fluorophore-specific wavelength. In most cases, emitted light has a longer wavelength, and therefore lower energy, than the exciting source, known as the Stokes shift [69]. Dr. Wu and colleagues applied NIR dye labeled Bevacizumab antibody to find out the pharmacokinetics, lymph Node Uptake and mechanistic PK behavior model after i.v. and s.c. administration in mice [70, 71]; they also found an interesting phenomena that after s.c. administration in mice, the lymph node uptake of proteins in mice had a molecular weight dependence by the way of fluorescence imaging [72]. This finding may provide quite useful aim for the future protein biodistribution and pharmacokinetic studies.
The most important application of optical imaging in the biological field is early cancer detection or diagnosis in preclinic and clinic studies, they provided a very useful alternative modality for tumor imaging [73]. Dr. Hülper P and his colleagues published their finding of tumor localization of nti-TGF-β antibody and its effects on gliomas. The orthotopic gliomas beared mice was subcutaneous injected with AF680-labeled 1D11, a pan-neutralizing TGF-β antibody, and used for the bio-distribution study via in vivo imaging and fluorescence microscopy. With the combination of results from direct tumor size measurement, H&E staining and immune-histochemistry, it demonstrate that TGF-β plays different roles in combating the tumor depending on subcutaneous versus orthotopic implantation site [74]. Besides the monoimaging principle, combining two or more different imaging modalities in the same agent can be of considerable value in molecular imaging. By binding a biotinylated anti-Her2 Herceptin antibody to provide tumor targeting, a biotinylated DOTA chelator labeled with 111In and a biotinylated Cy5.5 fluorophore to a streptavidin nanoparticle, the imaging results show high tumor accumulation and strong tumor-to-normal tissue contrast by both fluorescence and nuclear imaging. The subsequent biodistribution study confirmed the specific tumor accumulation of radioactivity at 40 h was 21 ID%/g and therefore much higher than all other tissues including liver, heart, kidney, spleen, and muscle that accumulated 8.7, 2.5, 6.9, 7.2, and 1.9 ID%/g, respectively [75]. In Cohen's work, IRDye800CW was coupled with monoclonal antibodies of cetuximab and bevacizumab with 89Zr multi-labeling for optical imaging of tumor targets. The biodistribution studies showed that blood clearance was enhanced and live uptake was increased at 24 to 72 hours post injection when 2 or more eq of dye had been coupled to mAbs. This study realized that after labeling with IRDye800CW, optical imaging can be used for accurate quantification of the biodistribution of mAbs (Figure 2) [76].
Optical imaging with 89Zr-bevacizumab-IRDye800CW in a tumor-bearing mouse. Adapted from [76].
In the area of cancer diagnosis, organic dye and semiconductor nanocrystals (so called quantum dots (QDs) or quantum rods (QRs) attract attentions because of its unique optical properties to be fluorescent probes or luminescent nanoprobes for biological applications, ranging from immunoassays to live cell and tissue imaging, especially in cancer detection [77-82]. Such nanomaterials have their advantages over traditional fluorescent probes such as organic dyes and fluorescent proteins [79, 83]. For example, they have robust photochemical stability, high quantum yield, and excellent resistance to chemical and photochemical degradation, as well as size-tunable photoluminescence that ranges from visible to near-IR with sharp spectral bands [84]. Also, QDs with different emission colors can be simultaneously excited with a single light source, with minimal spectral overlap, providing significant advantages for multiplexed detection of molecular targets [85, 86]. Thus, with conjugation with antibody and drug on the same QDs, this kind of theranostic reagent can be used as new type of probes for optical bioimaging and treatment on cancer.
Although there are significant advantages of semi-conductor nanocrystal QDs in the application of optical imaging for cancer detection, the potential toxicity cannot be ignored due to the existing of Cadmium element component inside the particles. In order to alleviate possible toxicity and increase the stability of QDs in biological field, Ding et al successfully employed carboxyl functional Pluronics F127 (F127COOH) into the antibody conjugated QDs for pancreatic cancer detection and obtained satisfied results. They successfully applied anti-mesothelin antibody in vivo for pancreatic cancer detection [87]. The in vitro and in vivo toxicity study showed the low damage to cells and tissues in small animals. Another alternative on optical imaging is using biodegradable polymer encapsulated NIR dye for cancer detection [88]. With proper fluorescent dye or drug encapsulation, this kind of biodegradable copolymer can be conjugated with target ligands or specific antibody for all purpose of tumor targeting. Because of the concerning about the possible toxicity, researchers also generated a "pure" bioconjugated luminescent silicon quantum dots for cancer detection [89, 90]. This kind of luminescent probe can even used for sentinel lymph node mapping since it is one of the most important site that the metastatic happened [91]. Although the above nanomaterials have their advantages over traditional fluorescent dye probes, batch to batch reproducibility and quantization of the labeled drug still need further investigation. Whether the biodistribution of the labeled therapeutic antibodies is significantly affected by the nanomaterials remains a question to be addressed.
Over the past years, different kinds of optical imaging techniques have been developed for biomedical applications, including various microscopy methods for in vitro and ex vivo applications as well as several methods for in vivo applications such as bioluminescence imaging, fluorescence imaging, ultrasound, dark field, diffused optical tomography, and optical coherence tomography, etc. Not only with help from expertise from studies on materials, various techniques can be used in combination, either simultaneously or sequentially, to provide complementary information from the same cells, tissues, organs, or animals [92-96]. Dr. Chen's lab applied fluorescent Maestro imaging system by real-time video imaging of protease expression in vivo, they found out that PEGylation of a molecular beacon with a specific size PEG can significantly alter the activation properties of the probe targeting matrix metalloproteinases (MMPs). And MMPs are a family of zinc-dependent endopeptidases and represent the most well-known proteases associated with tumorigenesis [97].
Among them, both fluorescence and bioluminescence imaging techniques have been found wide applications for in vivo tumor optical imaging in mouse models and afford convenient, frequent visualization and measurement of tumor biomarkers in a real time, sensitive, and noninvasive way. It also can allow for longitudinal detection of disease progression and therapeutic response in the same animals so as to minimize the subject-to-subject variability and reduce the animal number required by a traditional method. Importantly, such in vivo studies are very valuable for bridging the gap between in vitro and in vivo studies and facilitating preclinical and further translational studies [98].
6. Images guided engineered nanoparticles and antibodies cross blood-brain barrier (BBB)
The blood-brain barrier (BBB) is a special tight capillaries membrane in CNS that formed from a continuous layer of endothelial cells bound together with tight junctions that allow very little transcellular or pericellular transport of molecules in blood fluid [99]. The BBB is an obstacle for clinicians and complicates the treatment of diseases of the central nervous system (CNS). In order to develop BBB associated theranostic techniques, neuroimaging techniques have become increasingly important in assessing the biologic and physiologic properties of brain tumors and neurologic lesions (11). Although there are many successful imaging platforms including X-ray tomography (CT), optical imaging, positron emission tomography (PET), single-photon-emission computed tomography (SPECT), ultrasound (US), and magnetic resonance imaging (MRI), etc. MRI and combining with US seems the most successful applications in the clinic.
Nanoparticles of course can be employed as effective tool for theranostic purpose. One biocompatible stealth nanoparticles were designed by using microemulsion methodology to optimize BBB circumvention and encapsulate drugs for brain delivery. In vitro and in vivo data showed that the formulation has no effect on BBB barrier integrity and membrane permeability, or alteration of expression of the BBB tight-junction proteins, occludin, and claudin-1 while crossing BBB [100]. This kind of nanoparticles can be applied in theranostic through BBB. Nanoparticles encapsulation or conjugation of transporter substrates have already been used for enhance the access of drugs to the brain. In last decade, nano-sized carrier comprised by polymer, emulsion, liposome, nanocrystals, micelles and etc, are already make great progress in pharmaceutical field for drug targeting and imaging. Dr. Ding and his colleagues applied inorganic gold nanords for siRNA delivery into brain has been succeeded in vitro and in vivo based on their contrast property in cancer detection [64, 101-103]. They also applied fluorescent nanocrystal as theranostic carrier for CNS drug delivery system [104]. Dr. Koffie et al prepared biodegradable nanocarrier systems made up of poly(n-butylcyanoacrylate) dextran polymers coated with polysorbate 80 (PBCA nanoparticles) to deliver BBB-impermeable molecular imaging probes into the brain for targeted molecular neuroimaging. They demonstrated that PBCA nanoparticles using as in vivo targeting of BBB-impermeable contrast agents can deliver BBB-impermeable targeted fluorophores of a wide range of sizes: from 0.5k-Da targeted polar molecules to 150k-Da tagged immune- globulins into the brain of living mice model of Alzheimer's disease. PBCA nanoparticles do not induce nonspecific BBB disruption, but collaborate with plasma apolipoprotein E to facilitate BBB crossing [105]. Dr. Qiao et al conjugated lactoferrin (Lf) with poly(ethylene glycol) (PEG)-coated Fe3O4 nanoparticle to cross BBB by receptor-mediated transcytosis via the Lf receptor present on cerebral endothelial cells. The MRI images confirmed that in vivo experiments, Fe3O4-Lf probe exhibited an enhanced effective measure for delivering the nanoparticles across the BBB [106, 107].
It is well-known that few of those antibodies can penetrate the shield of tightly packed cells known as BBB. Normally, antibody concentrations in the brain are typically about a thousand times lower than in the blood. In the recent finding, Dr. Watts and his collaborators report the design of an antibody that surmounts this obstacle. This antibody had the function of targeting two proteins. One is called β-secretase 1, which is a popular target for drugs to treat Alzheimer's disease; the second protein targeted by the antibody is the transferrin receptor, which activates a molecular channel that normally imports iron into the brain. By clinging to this receptor, the antibody is transported into the brain, where it can act against β-secretase 1. The double-duty antibody performed well in mouse models of Alzheimer's disease: a day after receiving a single injection of the antibody, concentrations of amyloid-β in the brain plummeted by 47% [108, 109].
Lee et al applied peptidomimetic monoclonal antibodies that target peptide transcytosis systems for the BBB in vivo. Murine monoclonal antibodies to the rat transferrin receptor, such as the OX26 monoclonal antibody, are targeted through the BBB on the transferrin receptor in the rat. The capillary depletion technique demonstrated transcytosis of the RI7-217 rat monoclonal antibodies antibody can through the mouse BBB in vivo. The studies indicate rat monoclonal antibodies to the mouse transferrin receptor may be used for brain drug-targeting studies [110]. Dr. Boado and his colleagues used engineered murine monoclonal antibody to the human insulin receptor (HIR) as a brain drug delivery system for transport across the human BBB (Figure 4). The HIRMAb was humanized by complementarity determining region (CDR) grafting on the framework regions (FR) of the human B43 IgG heavy chain and the human REI kappa light chain. The humanized HIRMAb avidly bound to the HIR of isolated human brain capillaries, which are used as an in vitro model system of the human BBB. The humanized HIRMAb was radiolabeled with 125-iodine, and injected intravenously into an adult, anesthetized Rhesus monkey. Brain scanning showed the humanized HIRMAb was rapidly transported into all parts of the primate brain after intravenous administration. The humanized HIRMAb may be used as a brain drug and gene delivery system for the targeting of large molecule therapeutics across the BBB in humans [111].
Film autoradiography of Rhesus monkey brain removed 2 h after an intravenous administration of [125I]-humanized HIRMAb. Coronal sections through the forebrain (top panel), midbrain (middle panel), and hindbrain/cerebellum (bottom panel) are shown. Adapted from [111].
Molecular imaging takes advantage of the traditional diagnostic imaging techniques and introduces molecular imaging probes to measure the expression of indicative molecular markers at different stages of diseases. It has been widely reported the importance and advantages of various imaging modelities in the drug development. We summarized the distribution and pharmacokinetics studies using MR imaging, CT, PET imaging, SPECT imaging, optical imaging as well as SPECT etc. Among those imaging modalities, PET, SPECT and MRI imaging has been used in clinical study, while optical imaging is still on the way of getting through; almost all of the studies using optical imaging are in the preclinical status. Non-invasive detection of various molecular markers of diseases can allow for much earlier diagnosis, earlier treatment, and better prognosis that will eventually lead to personalized medicine. For using molecular imaging to investigate the biodistribution and PK properties of theranostics, the following aspects still need further investigation:
Major advantages of radionuclide-based molecular imaging techniques (SPECT and PET) over other modalities (optical and MRI) are high sensitivity (picomolar level), quantitation, and non-tissue penetration limitation. However, one disadvantage is that the resolution of either SPECT or PET is not as high as MRI. In most cases, the modalities such as PET or SPECT can be applied for studies of biodistribution, PK study properties, and/or tumor-targeting efficacy in non-invasive image ways.
Comparing with other medical imaging techniques such as CT, MRI provides good contrast between the different soft tissues of the body, which make it especially useful in imaging brain, muscles, the heart, and cancers. CT is preferable in the application of bones imaging while MRI is good for tissue imaging.
For both PET imaging and optical imaging, the image only showed the signals from the radioisotope probe or fluorophore probe, while the probe may still attach to the metabolite of drug even after the degradation of the drug. Therefore, the imaging method is not able to distinguish the metabolite or parent drug. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) or size exclusion HPLC for confirming the stability of the labeled antibody therapeutics may be a good way of providing more information of metabolism. For labeled antibody, due to its therapeutic effect and detective property, the labeled antibody can also be considered as theranostics if it is used in the field of treatment and diagnosis.
After obtaining biodistribution data using molecular imaging in preclinical study, establishing physiologically based pharmacokinetic model (PBPK) and allometric scaling for translation of animal data to human clinical pharmacology is very important. Further research effort is needed on establishing PBPK model using imaging data.
For the later clinical application, the absorption, distribution, metabolism/degradation and excretion of theranostics need to be extensively studied. Various imaging modalities mentioned in this review are useful quantitative tools for investigating the above biopharmaceutical properties of theranostis.
BBB, blood-brain barrier; CDR, complementarity determining region; CT, computed tomography; DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; ELISA, Enzyme-linked immunosorbent assay; FR, framework regions; HIR, human insulin receptor; HPLC, high-performance liquid chromatography; Lf, lactoferrin; mAbs, monoclonal antibodies; MRI, magnetic resonance imaging; NETs, neuroendocrine tumors; NIRF, near-infrared fluorescence; PBCA, poly(n-butylcyano- acrylate) dextran polymers coated with polysorbate 80; PBPK, physiologically based pharmacokinetic model; PET, positron emission tomography; ROI, region of interest; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; SPECT, single-photon emission computed tomography; TATE, octreotate; US, ultrasound; USPIO, ultrasmall superparamagnetic iron oxide; VEGF, vascular endothelial growth factor.
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Corresponding author: Hong Ding, Department of Immunology, Florida International University, Miami, FL33199. Tel: 305-348-1490, Fax: 305-348-1109. Email: hdingedu.
Accepted 2012-6-17
Published 2012-11-5

redpajama_set_name: RedPajamaCommonCrawl


text:
Daily Devotional Friday 8th May 2020
Acts 16:11–15
The Conversion of Lydia
11 So, setting sail from Troas, we made a direct voyage to Samothrace, and the following day to Neapolis, 12 and from there to Philippi, which is a leading city of the1 district of Macedonia and a Roman colony. We remained in this city some days. 13 And on the Sabbath day we went outside the gate to the riverside, where we supposed there was a place of prayer, and we sat down and spoke to the women who had come together. 14 One who heard us was a woman named Lydia, from the city of Thyatira, a seller of purple goods, who was a worshiper of God. The Lord opened her heart to pay attention to what was said by Paul. 15 And after she was baptized, and her household as well, she urged us, saying, "If you have judged me to be faithful to the Lord, come to my house and stay." And she prevailed upon us.
[1] 16:12 Or that
In Philippi, Paul begins his outreach among those who were religious Jews (v13). These religious people could be described as 'low hanging fruit', people easier to reach because they would have a knowledge of the Old Testament and who God was.
Yet, for even religious people to be saved it takes a miracle as we now see in Lydia (v14), whose heart was opened to receive the Gospel message. For anyone to respond to the Gospel and be saved, it takes a work of grace in transforming a hard heart of sin into one that will accept Christ as Saviour and Lord. The prophet Ezekiel speaks of how the Lord will take our hard heart of stone which resists Christ, and replaces it with a soft heart of flesh, that is open and sensitive to Jesus and the Gospel (Ezekiel 36 v26).
Lydia's new found faith and love for the Lord is evident in her and her family being baptised but also in her support for Paul through hospitality (v15).
Genuine faith should be seen in caring for God's people and supporting Gospel ministry. True faith will have hands that will serve and legs that will go for the sake of the Gospel. Pray about how you are involved at present in supporting Gospel ministry and those who minister. Is there something more the Lord wants you to do?
More from May 2020
Acts 16v6-10
Acts 16v16-24


Did you know that "The Hamptons" are a collection of small villages and hamlets that includes: East Hampton, Bridgehampton, Southampton, Westhampton, Sagaponack, Sag Harbor, Watermill and a few others.
The reason so many celebrities take refuge in The Hamptons is that you will not find any paparazzi, strip malls, or event a larger grocery store and definitely not a Walmart. What you will find are close-knit communities of small business owners, boutiques, cafes, and events that gather people together. Community is often lost in our society and we all yearn for it. The Hamptons towns and villages have that sense of purpose. The Hamptons might seem to be the place just for the very rich and famous but we felt right at home when we visited.
Since we had never been to Southampton we decided to stay in a well-known Inn called the Southampton Inn. The Southampton Inn is conveniently located just out of the main town on 91 Hill Street. It is quite affordable at around $199 a night during the week, off-peak. That's reasonable for this area are you will not find many chain hotels in the towns. There are some outside of town about 15 to 20 minutes away. When you stay in the Hamptons you are looking for the entire experience so staying at a local Inn or Bed & Breakfast fulfills that need.
The first day on our trip, we ventured into the town and our first stop was a lovely coffee shop and cafe at 99 Main Street called The Golden Pear Cafe. The Golden Pear Cafe offers breakfast, pastries, quiche, sandwiches, salads, and great coffee anytime of day. When you open the door of The Pear Cafe you immediately feel welcomed by the smell of fresh pastries and coffee. The Pear Cafe is the perfect stop for coffee and we enjoyed it so much we came back the next day for lunch.
There are so many things to do in Southampton that cost little or no money. This can definitely be an affordable trip. Window shopping is a favorite, people watching, driving around and "house gawking" and walking by the ocean are some of our favorite things to do and they don't cost a dime. Of course, once you are in some of the shops you may want to open your wallet but that is completely optional.
You will find lots of people out walking their dogs. Make sure to stop by Ralph's Barber shop on Main Street and say hello to Buddy, the resident Yellow Lab. He might even do a trick for you.
Walking down Main Street you will walk past small boutiques, dress shops, a cheese shop, galleries, the local barber and Hildreth's Home Goods Department Store. Hildreth's (51 Main Street) is "America's oldest store" and has been around since 1842. This is where you want to go for celebrity spotting and to get inside information from the locals about the town. Celebrities often shop at Hildreth's because it has everything – furniture, children's, bed and bath, tabletop, outdoor, home decor, gifts, notions like yarn and fabric and even a toy store.
If you are looking for a quick bite to take back to your hotel , stop in The Village Gourmet Cheese Shoppe for on-the-go cheese platters, Smoothies, or an Espresso.
Once you have spent the day in town, make reservations for dinner at 75 Main. 75 Main has been recognized as the number 1 Italian restaurant in The Hamptons. When the weather is nice, you will find guests dining outside and if you are eating inside, they open the doors to let the fresh air in.
If the weather is good you must drive down to Cooper's Beach on Meadow Lane. Parking is free in the Fall after Labor day and you can spend an afternoon walking on the beach, picking up shells, walking the dog, or watching the waves come in.
Driving past Cooper Beach you will see beautiful homes lining both sides of the streets. Some of the ornate gates are just as beautiful as the houses and you might want to stop and wonder who lives there.
With so much to see and do, a trip to Southampton, New York, must be on your itinerary for the Fall.


Francis Marshall Hall, 92, of Virginia Beach, died at his home Sunday, March 24, 2019. Francis was born Nov. 7, 1926, in Norfolk to Spotswood Braxton Hall and Harriet Hicklin Hall. He grew up in Bronxville, New York, and moved to Virginia Beach in the 1950s.
Francis was preceded in death by his wife, Elizabeth Hitch Hall; and brother, Spotswood Braxton Hall Jr.
He is survived by his children, Francis Marshall Hall Jr. (Gail) and Margaret Rives Hall Bacon (Jesse); grandchildren, Dr. Mary Adams Bacon, Margaret Lee Bacon, Thomas Nash Bacon, Page Spotswood Bacon, Carter Francis Hall and Katharine Rives Hall; and his cat, Annie.
After graduating from Bronxville High School, Francis entered Yale University, but withdrew in early 1945 to join the U.S. Army Air Force as an aviation cadet, training in Texas and Colorado. After the war ended, Francis returned to Yale, graduating in 1949. After graduation, Francis worked briefly in the coal business, but was recalled to the Air Force for stateside duty during the Korean War.
After his second discharge, he worked several years as an engineer for a gas company before beginning a long career in the securities brokerage business, starting as a stockbroker in Norfolk and then moving to various management roles in Norfolk and Richmond.
Francis enjoyed athletics in high school and college, competing in basketball, swimming, track and other sports. Over the years, he also enjoyed sailing, fishing, boating and building furniture and model ships. Francis competed in Masters Swimming until he was 85 and was a longtime fan of UVA basketball.
A graveside service will be held at 1 p.m., Friday, March 29, at Eastern Shore Chapel Cemetery followed by a reception in the parish hall.
We would like to express our thanks to the wonderful staff at First Colonial Inn, which was his home for the past two years.