text:
Jack Lake ski trails groomed, but there still is a demand for plenty more snow
Langlade County recreation officials are urging patience as the winter season gets into swing.
Although it seems as though winter started early, the white stuff has been light and fluffy and temperatures have been exceptionally frigid, not the ideal ingredients for cooking up a great batch of skiing and snowmobiling.
Snowmobile trails in the wooded areas of the county are opening tonight while Kettlebowl will fire up the tow ropes a week from today, weather permitting. And the county's main cross-country ski trails are open.
But no one should expect ideal, mid-winter conditions.
"For the most part, people understand early season limitations," Erik Rantala, Langlade County forest administrator, said. "We do the best we can certainly, but there are challenges that come with limited snow early in the season."
Jack Lake has a well-deserved reputation as the area's premier cross-country system, but until enough snow falls for the big Pisten Bully to be fired up, grooming must take place the old-fashioned way, on a Ski-Doo Skandic towing an array of rollers and packers over the 20 miles of loops.
Jack Lake has a well-deserved reputation as the area's premier cross-country system, but until enough snow falls for the big Pisten Bully to be fired up, grooming must take place the old-fashioned way, on a Ski-Doo Skandic towing an array of rollers and packers over the 20 miles of loops
That's a slow process.
"Getting the whole trail packed takes days and days," Tom Lazers, whose duties as the Veterans Memorial Park caretaker includes trail grooming duties at Jack lake and Kettebowl. "There is a tremendous amount of time involved just in getting the trail started."
Lazers generally grooms at Jack Lake daily, Tuesdays through Thursdays, and spends Fridays at Kettlebowl. Problems can arise when late Thursday and Friday snowfalls hit, blurring the trails for the weekend, when skiers really want to get out and enjoy them. Rantala said his department is working to address those issues with extra manpower for grooming duties when needed.
Lazers said the trail needs an additional six to eight inches of snow, and then the big machinery can be fired up, reducing trail grooming time from days to hours.
"You've got to have a good base or you'll wreck the trail and wreck the machine," he said. "By mid-January, this trail will be unbelievable, but it takes a long time to get there."
The county's shorter systems, operated by volunteers at Gartzke Flowage and Moccasin Lake, have a shorter turn-around time for packing and grooming, but early season conditions still exist.
At Moccasin Lake, the trail will be fine for classical skiing this weekend, but equipment problems will delay a new round of grooming and packing until sometime next week.
Gartzke Flowage volunteers have been grooming and combing the trails and tracks may be set for the weekend.
The city of Antigo's in-town trail has been packed, but not tracked, while in eastern Langlade County, the Maranatha trail system at Silver Birch Ranch is expected to be open for Christmas if the snow cooperates.
Langlade County does not require passes for trail use, instead relying on volunteers. Donation boxes are located at the entrances of the Moccasin and Gartzke trails and volunteers are always welcome to join the Elcho Cross-Country Ski Club and the Antigo Bike and Ski Club, which operate the systems.
At Jack Lake, instead of always plugging the donation box, the county is again encouraging regular skiers to buy zipper pulls to wear on their ski jackets. The brightly-colored pulls read "I Ski Langlade County: Jack Lake 2013-14" and are available for $25 from the forestry office located at the fairgrounds. People may also send in their donations to the office located at 1633 Neva Rd. and have the tags mailed to them.


Please note that textbooks and materials are not included within the tuition fees. For a complete listing of required textbooks, materials and uniforms, please refer to the Abrams College bookstore pamphlet for a complete description.
Students must be aware that there are government agencies that charge fees for licensing or certification for certain professional occupations. Although nominal, the student must take into account that these fees are paid directly by the student to the specific government agency.
To be successful in the pursuit of an education, the student must take into account other related fees associated with training and education. These fees include cost of housing, transportation to and from school, health insurance, meals and entertainment as well as emergencies that may arise from day to day.
Abrams College accepts payment for tuition in the form of Cash, Money Order, Certified Bank Check and credit cards. For credit card payments, there is a small fee for administrative charges. Sorry, no personal checks.


Local board members from around Oregon, with support from OSBA and its Board of Directors, have established a new Oregon School Board Members of Color Caucus.
Encouraging individuals of color to run for school boards.
More information about the caucus, including membership options, is available on the OSBA website.
Also a workshop about the caucus is planned for 11 a.m. Saturday, Nov. 12, during OSBA's 70th Annual Convention in Portland. Convention registration materials can be found here.


Black and white bedroom decor lovely 35 affordable black and white bedroom ideas decorationy.
Black and white bedroom decor lovely how to create stunning interior design in black n white.
Black and white bedroom decor luxury wonderful bedroom decor ideas in black and white home design.
Black and white bedroom decor new modern black and white bedroom ideas.
Tags: black and white bedroom decor, black and white polka dot bedroom decor, green black and white bedroom decorating ideas, black and white living room home decor.


text:
Q: In the second round of Invasion: Spire, where does the shotgun spawn? In the second round of Invasion: Spire, when the Spartans are trying to capture the points at the bottom of the spire, I have seen people using the shotgun, but none of the loadouts have it so it must spawn on the map. Does anyone know where the shotgun spawn is for this round?

A: If you're coming from the Spartan spawn, the shotgun is on the far right side, leaned up against a metal fence.  There's also a rocket launcher, nearest the left spawn, and a sniper rifle up against the rocks near the middle spawn.

redpajama_set_name: RedPajamaStackExchange


Lagos, Nigeria- Nigeria's largest and leading lifestyle fair style by ZENITH 2018 took place over the weekend on Saturday, December 29 and Sunday, December 30, 2018 at the Open Ground, Oniru, Victoria Island; bringing together fashion, entertainment and lifestyle finest for what can best be described as the "Lifestyle, Fashion and Entertainment fair of the year".
Championed by leading influencers Alex "Unusual" Asogwa, Denola Grey, Uti Nwachukwu and Steve Chuks who brought on great style to the event; the fair featured an explosive two-day convergence of connoisseurs, vendors and enthusiasts in lifestyle, fashion, entertainment, beauty, automobiles and food industries.
The fair featured masterclasses anchored by TV personality Bonang Matheba; international supermodels Oraine Barrett and Tarmar Awobotu; vendors' exhibition; runway shows and daily music concerts with performances by Waje, Adekunle Gold, Simi, Mayorkun, Timaya, Kizz Daniel, Wande Coal, 2Baba and Bobby Valentino.
The fair, which had attendees throng the venue in their hundreds, was hosted by Mercy Ajisafe, Eku Edewor and Ill Rymz who all brought on their finesse to make the fair an exhilarating show and a perfect way to shut down the year.
Highlights of the fair include the super runway shows walked by only the best of models who strutted their stuff in pieces from hand-picked premium fashion houses; guest appearances from Tobi Bakre, Akin Faminu, Laura Ikeji, Emmanuel Ikubese, Anita Adetoye and the impromptu on-stage dance performance by former Big Brother Naija housemate Alex "Unusual" Asogwa which sent fans into a frenzy.
Virtual Xchange Limited, strategic partner Africa for international premium fashion, lifestyle and entertainment television network, Fashion | One, collaborated with Zenith Bank Plc, on the latter's flagship lifestyle and fashion fair, "Style by ZENITH".
Fashion One operates a global lifestyle and entertainment TV network offering comprehensive coverage of inspirational fashion. Fashion One audiences can access the latest updates through high-quality original programs, reality shows, documentaries, travelogues, entertainment news and lifestyle magazines.
Zenith Bank PLC is a Nigerian multinational financial services provider. It is licensed as a commercial bank, by the Central Bank of Nigeria, the central bank and national banking regulator. Zenith Bank is a leading financial service provider in Nigeria and Anglophone West Africa.
Zenith Bank Plc was established in May 1990, and commenced operations in July of the same year as a commercial bank. The Bank became a public limited company on June 17, 2004 and was listed on the Nigerian Stock Exchange (NSE) on October 21, 2004.


You can easily make your own jar full of Fire Flies or Lightning Bugs with just a few solder joints. The lights flash on and off just like a fire fly and you can change how long they stay on and how long they are dark between flashes. All it takes is an Arduino, 3 LEDs, 3 resistors, a battery, and a Jar.
some assorted wire, wire cutters, soldering iron, and solder.
Find the short leg of your LEDs. This is negative, also known as the cathode. Solder a resistor to this leg, you can cut it short first if you like.
Repeat to make 3 "fire flies."
Download a FireFlyJar .ino file from GitHub . This is the program (or sketch) that lets your Arduino power the Fire Fly Jar.
FireFlyJar-Continuous-0.1.ino will light your LEDs up as long as you have it powered.
FireFlyJar-Timeout-0.2.ino allows you to set a timeout for the LEDs. After a set time they will stop flashing and put the Arduino in shutdown mode. It will continue to draw power but MUCH less.
Start the Arduino program and open the file you downloaded from GitHub.
Plug your Arduino into the USB on the computer. The red light should come on.
Click on the little right arrow in the upper left corner next to the check mark.
You should get a notice that the software is uploading and the red light on the Arduino should blink.
You're done. The Arduino is now programmed.
Disconnect the USB cable from the Arduino before soldering!
Take the positive leads from the LEDs and solder one each into the holes D9, D10, and D11.
It might be easier to tin the wire first. To do this just twist the wire a little and put a small amount of solder on it.
Wire the 3 negative lines together and solder one to the hole marked GND on the Arduino.
With a battery attached (or the computer) the LEDs should start blinking.
When the paint is dry arrange your LEDs in the jar.
Mount the battery under the lid and the Arduino on top of the battery. I used double sided foam tape to hold it all together (and prevent the Arduino from shorting out on the battery.) I had to mount the Arduino on top otherwise the battery would hold down the reset button.
I wrapped it all in electrical tape just for a little extra security.
nice. I made one following the instructions. Learned a lot from this project.
This is really cool. I love the finished look of the jar. Very nicely done!
Thanks. It's my first Instructable and still under construction. Come back later for updates such as auto-off and wiring in a switch.


Harold's exposure to the industry began at a young age while working after school and summers at his parent's real estate firm in Amarillo. Harold earned a BBA in Entrepreneurship and Management from Baylor University. He sharpened his communication and presentation skills as a corporate trainer during his 8 years with ExecuTrain. Then he returned to his real estate roots, now with over 15 years in home financing.
Harold is dedicated to listening to clients, then educating them on best options based on their specific needs and situation. He recognizes the value of delivering beyond the expected. His knowledge about the home buying process does not stop at home finance. His ability to provide outstanding customer service comes from his complete understanding of the buying and selling process and strategies. He creates raving fans and is blessed to have numerous families he has helped repeatedly over the years.
Harold regularly presents to groups on the topics of home finance, credit reports, home buying process, and strategies to help clients and realtors. He frequently works in coordination with financial planners to help mutual clients with real estate needs.
Harold and his family are actively involved at The Village Church – Dallas Campus where he and his wife, Shelby, serve as Deacons, Home Group Leaders, and several other volunteer roles. Harold serves on the Board of Dallas Life Homeless Ministry, the largest homeless shelter in North Texas providing food, shelter, clothing, education, medical care, and long-term rehabilitation. Harold is a regular in the early morning class at Orange Theory Fitness. Harold and Shelby have three children and are often involved in their school, athletic, or scouting events.


text:
Case Name EEOC v. PORT ELIZABETH TERMINAL & WAREHOUSE EE-NJ-0074
Docket / Court 05-4828 ( D.N.J. )
State/Territory New Jersey
Case Type(s) Equal Employment
Special Collection IWPR/Wage Project Consent Decree Study
Attorney Organization EEOC
On October 5, 2005, the Equal Employment Opportunity Commission ("EEOC"), on behalf of a customer service representative ("CSR"), filed a lawsuit in the District Court of New Jersey, under Title VII of the Civil Rights Act of 1964, as amended, and the Age Discrimination in Employment Act ("AEA"), ... read more >
On October 5, 2005, the Equal Employment Opportunity Commission ("EEOC"), on behalf of a customer service representative ("CSR"), filed a lawsuit in the District Court of New Jersey, under Title VII of the Civil Rights Act of 1964, as amended, and the Age Discrimination in Employment Act ("AEA"), against Port Elizabeth Terminal and Warehouse. The EEOC sought injunctive relief, compensatory damages, punitive damages, attorneys' fees, and other forms of affirmative relief, claiming that the defendant treated the complainant less favorably than younger, non-black CSRs, eventually resulting in her discharge after she complained about the disparate treatment.
On June 15, 2006, the District Court (Judge William J. Martini) entered a consent decree where the defendant, among other things, agreed to pay the complainant $28,500.
Perry Miska - 04/13/2014
Develop anti-discrimination policy
Discrimination Prohibition
Implement complaint/dispute resolution process
Post/Distribute Notice of Rights / EE Law
Provide antidiscrimination training
Retaliation Prohibition
Discrimination-area
Discharge / Constructive Discharge / Layoff
Other Conditions of Employment (including assignment, transfer, hours, working conditions, etc)
Discrimination-basis
EEOC-centric
Direct Suit on Merits
Disparate Treatment
EEOC Plaintiff
Causes of Action Age Discrimination in Employment Act (ADEA), 29 U.S.C. §§ 621 et seq.
Title VII (including PDA), 42 U.S.C. § 2000e
Defendant(s) PORT ELIZABETH TERMINAL & WAREHOUSE
Plaintiff Description Equal Employment Opportunity Commission, on behalf of a customer service representative who was allegedly discriminated against based on her age and race.
Indexed Lawyer Organizations EEOC
Class action status sought No
Class action status outcome Not sought
Nature of Relief Damages
D.N.J.
06/15/2006 2:05-cv-04828-WJM-RJH
EE-NJ-0074-9000.pdf | Detail
Complaint [ECF# 1-1]
Order of Dismissal [ECF# 10]
Consent Decree [ECF# 11]
Judges Martini, William J. (D.N.J.) show/hide docs
EE-NJ-0074-0002 | EE-NJ-0074-0003 | EE-NJ-0074-9000
Plaintiff's Lawyers Marino, Stephanie (Pennsylvania) show/hide docs
McNair, Jacqueline H. (Pennsylvania) show/hide docs
EE-NJ-0074-0001 | EE-NJ-0074-0003
O'Boyle, Judith A. (Pennsylvania) show/hide docs
Defendant's Lawyers Conlan, Michael J. (New Jersey) show/hide docs

redpajama_set_name: RedPajamaCommonCrawl


text:
Books and Literary
Nonprofit Events
Times Union Events
Duanesburg
East Greenbush
Scotia-Glenville
The Philadelphia Orchestra: Beyond Baroque
August 1, 2019, 2:00 pm at Saratoga Performing Arts Center, 108 Avenue Of The Pines, Saratoga Springs, NY
Price: $18-63
spac.org
Leopold Stokowski, known for his long association with The Philadelphia Orchestra, was a cultural pioneer, bringing the latest in art and music to Philadelphia in the early 1900s and presenting it in bold, new ways – most notably his incredible interpretations of famous baroque works.
This robust program features Bach's Toccata and Fugue in D, which has become a vastly popular piece, especially in the horror genre, and has been prevalent in pop culture since well before the sound film era. Although it has been a revered work in the classical cannon for more than a century, Bach's Toccata and Fugue in D saw renewed popularity from an arrangement by Stokowski and The Philadelphia Orchestra used in the 1940 animated film Fantasia.
New York City Ballet: Coppélia
1 New York City Ballet: Coppélia
Saratoga Performing Arts Centerz108 Avenue Of The Pines, Saratoga Springs, NYa
New York City Ballet Matinee: Coppélia
2 New York City Ballet Matinee: Coppélia
SPAC's New York City Ballet Gala
3 SPAC's New York City Ballet Gala
SPAC ON STAGE SERIES: Tony Succar & Mixtura
4 SPAC ON STAGE SERIES: Tony Succar & Mixtura
Fela! The Concert
5 Fela! The Concert
The Philadelphia Orchestra's Opening Night @ SPAC
6 The Philadelphia Orchestra's Opening Night @ SPAC
BING BAMBOO ROOM BURLESQUE ONCE UPON A TIME IN SPACE SATURDAY JULY 20, 2019
Hudson Harbor House 351 Broadway Albany, NY 12207351 Broadway, Albany, NY 12207, United Statesa
Watercolors by OTTO Exhibition
Hudson Area Library51 N. Fifth St., Hudson, NYa
Contained Exuberance
Berkshire Botanical Garden5 W Stockbridge Rd, Stockbridge, MA 01262, United Statesa
Lucy's Garden, A Topiary Collection.
Shimmering Flowers: Nancy Lorenz's Lacquer and Bronze L ...


text:
package log

import (
	"github.com/apache/servicecomb-service-center/pkg/util"
	stlager "github.com/go-chassis/paas-lager"
	"github.com/go-chassis/paas-lager/third_party/forked/cloudfoundry/lager"
	"runtime/debug"
)

var (
	defaultLagerConfig = stlager.DefaultConfig()
	stdOutWriters      = []string{"stdout"}
	fileWriters        = []string{"file"}
)

// Config struct for lager and rotate parameters
type Config struct {
	LoggerLevel   string
	LoggerFile    string
	LogFormatText bool
	// MB
	LogRotateSize  int
	LogBackupCount int
	CallerSkip     int
}

func (c Config) WithCallerSkip(s int) Config {
	c.CallerSkip = s
	return c
}

func (c Config) WithFile(path string) Config {
	c.LoggerFile = path
	return c
}

func Configure() Config {
	return fromLagerConfig(defaultLagerConfig)
}

func fromLagerConfig(c *stlager.Config) Config {
	return Config{
		LoggerLevel:   c.LoggerLevel,
		LoggerFile:    c.LoggerFile,
		LogFormatText: c.LogFormatText,
	}
}

func toLagerConfig(c Config) stlager.Config {
	w := fileWriters
	if len(c.LoggerFile) == 0 {
		w = stdOutWriters
	}
	return stlager.Config{
		Writers:       w,
		LoggerLevel:   c.LoggerLevel,
		LoggerFile:    c.LoggerFile,
		LogFormatText: c.LogFormatText,
	}
}

// newLog new log, unsafe
func NewLogger(cfg Config) *Logger {
	if cfg.CallerSkip == 0 {
		cfg.CallerSkip = globalCallerSkip
	}
	stlager.Init(toLagerConfig(cfg))
	return &Logger{
		Config: cfg,
		Logger: stlager.NewLogger(cfg.LoggerFile),
	}
}

type Logger struct {
	Config Config
	lager.Logger
}

func (l *Logger) Recover(r interface{}, callerSkip int) {
	l.Errorf(nil, "recover from panic, %v", r)
	l.Error(util.BytesToStringWithNoCopy(debug.Stack()), nil)
}

func (l *Logger) Sync() {
}

redpajama_set_name: RedPajamaGithub


Rio Rio 900 Firmware v1.63 Windows 98/ME/2000. This is the original firmware release that shipped with the Rio 900.
Rio Nitrus/Eigen Firmware v1.23 Windows 98SE/ME/2000/XP. Resolved an issue whereby some purchased WMA tracks experienced a skipping or stutter when played back.


text:
Rhodri ab Idwal or Ithel ('Roderick son of Idwal') is a Welsh name that may refer to:

 King Rhodri Molwynog of Gwynedd (8th century)
 King Rhodri ab Idwal (Glywysing) of Glywysing (9th century)
 Prince Rhodri ab Idwal Foel of Gwynedd (10th century)

redpajama_set_name: RedPajamaWikipedia


Think professional development meets inner peace! Experience a blissful, Zen evening for your salon, AND learn valuable beginner meditation skills for life.
Relax, unwind, center and re-energize for calm behind the chair AND in the salon. A team-building beginner meditation experience!
$40 per person for a 90 minute session. 4 person minimum -10 max. Meditation CD included for each participant.
Currently offered within a 20 mile radius of Pittsboro, NC. Further than that? No worries! You can still book the evening, along with an extra travel fee.
4 person minimum – 10 max. CD included. Only $32 per session!
Bi/weekly and monthly packages also available.
Fit CALM in to your salon on your time and schedule – no excuses.


When you're a true child of God, you have surrendered your life to God. You have surrendered all like that song writer says; any power opposing you are running against an immovable stone. If they strike that stone, they will end up sustaining injuries. If they sufficiently provoke a stone, and the stone decide to move against them, it will grind them to powder.
So two ways: it's either you fall upon that stone, or this stone falls upon you. Confronting a true child of God will reduce the confronter to dust that it may be scattered to winds. To engage the children of the stone, is to write a courier letter to irredeemable destruction.
In you there is power.
It says behold, I lay in Zion a stumbling block, if anyone falls on that rock, it shall be broken into pieces.
The problem now comes, who are the children of the stones, because they are the ones we must not mess up with.
In 1994 when MFM started here, all the native doctors here gathered together and held meetings for days, they were calling my name (it was one of them that came to confess). When they kept on calling, a stone appeared before them, they spoke to the stone, and it didn't move. They closed for the day, the next day, they still called my name and saw the stone. They spoke incantations, the stone didn't move. In day three, an elderly man in their midst summoned them and told them that this man of God you called, and stone appeared yesterday and today, if you call again the third time and it appear, they will all be gone out of this world. So they closed the case and their agenda.
You must have heard me sharing this, I (the GO) need to share it again.
This woman will get pregnant and at certain month of pregnancy, in her dream, a wild cow will appear before her in the dream and use his horn to move towards her forcefully and puncture her stomach, the next day, the baby is dead. Someone brought her to this meeting, then PMCH has not started then. She heard me saying a scripture in Matthew 22:44. I taught them one prayer point that: power attacking my life, collide with the rock of ages in the name of Jesus! She was pregnant then. That night, she had a dream again and the cow made the attempt he regularly makes, when it remain a slightest flint to hit her, a stone appeared and it collided with the stone. The next day, her chief bride when she wedded, who have always come to sympathize with her anytime she lose the baby; she died a violent death, with all her skulls broken.
1. Problem number 1 is what we find in psalm 78:41-51. This is everything God has done, but they limited God. It is a very sad situation, they turned God into a limited liability company which could fall into debt and closure. They forgot that God is a man of war. The same people God saved began to accuse God, and God always get angry when men put a limit on Him. The sin of limiting God is a common sin among Christians. Unbelief is an insult to the power of God. There was a man called Winston Churchill. He was an intellectual block head. He was mocked and was given an award for the positions of dons and idiots. This same man was made a prime ministers and he was head over those who mocked him then. Every tongue that has limited your destiny is dried up now in the name of Jesus! Any satanic arrow that has determined to be part of your life, I kill it now in the name of Jesus. Do you know why some of us have problems? It is because we engage the devil in discussions. All those why in your life are all questions from the devil. Satan has always asked questions but he doesn't ask any honest question. After a service so powerful, he will come and ask you do you think that prophesy upon your life will come to pass? If you don't keep off from that question, very soon he will begin to suggest his own prescriptions to that your problem. Abram meant exalted father, God changed his name to Abraham which meant father of many nations. So as God removed abram from his (Abraham) life, God will remove every abram in your business, life and career in the name of Jesus!
2. Problem number 2 is that A lot of people are being invited to connect to the rock of ages but then, there are certain rough edges God need to remove from their lives. Many of us are suffering from what is called spiritual immaturity. Many Christians give very little time to devote to the study of the word of God but they devote a greater attention to their career studies. Woe to God that our priorities change as believers, that's why the New Testament lament to your commitment to the word of God.
Bottomline: For the grinding stone to work for you, you must not limit God, you must be a man of God, His word must dwell richly in you.
Bow down your head and pray this prayer: Anything in my life that will prevent the rock of ages from fighting for me, no matter how I love it, take it away from my life.
1. Raise up your hand to the heaven-lies: In this month, whether the enemy likes it or not, I shall prosper by fire in the name of Jesus.
2. Thou power of opportunity wasters, I'm not your candidate, die in the name of Jesus.
3. Anywhere I appear this month, darkness shall disappear in the name of Jesus.
4. All my breakthroughs in captivity, be delivered in the name of Jesus.
5. In whatever I'm passing through now, oh God: answer your name in my life in the name of Jesus.
6. Oh God arise and scatter the decisions of my enemies in the name of Jesus.
7. Sources of my battles, what are you waiting for? Die in the name of Jesus.
8. Every evil hand upon my life assigned to scatter my progress, die in the name of Jesus.
9. Any power that wants suffering to be my signature, oh God arise, scatter them in the name of Jesus.
10. Powers rising up early to curse me, oh God arise, tear them to pieces in the name of Jesus.
11. Satanic lions assigned to tear my destiny, oh God arise, tear them to pieces in the name of Jesus.
12. Oh God arise (shout it three times), make me unstoppable in the name of Jesus.
13. Wicked prophets, wicked witch doctors assigned to mock my destiny, oh God arise, tear them to pieces in the name of Jesus.
14. Every problem left in this year shall not rest on my shoulders in the name of Jesus.
15. Whenever I knock a door, oh Heavens, open seven doors unto me in the name of Jesus.
16. Powers assigned to kill my destiny in the room of battle, what are you waiting for? Die in the name of Jesus.
17. Any power feeding a tree against me, die with your tree in the name of Jesus.
18. Close your eyes and think about the warfare projected into your dream life and shout this: warfare in my dream, stone of fire, kill them now in the name of Jesus.
19. Powers contesting for my joy, collide with the rock of ages in the name of Jesus.
20. Say I connect my life to the grinding power of the rock of ages (3 hot times) in the name of Jesus.
21. Powers consulting sand to fight against me, rock of ages, grind them to powder in the name of Jesus.
22. Any power saying my battle will not end, die in the name of Jesus.
23. Every agent of bad luck, carry your load in the name of Jesus.
All the men in this service please listen carefully, all the wives in this service; pass this information to your husbands whether born again or not: Next Sunday @ the international headquarters, we shall anoint all the hands of all the men in the service. This will be a continuous process in the next three sunday services. There are men who have giant breakthroughs in their hands and the enemy makes it slip out of their hands mysteriously.


text:
Dana Gillespie's 2021 memoir, Weren't Born A Man (Hawksmoor Publishing), lifted the lid on one of entertainment's wildest, most adventurous and creative lives. Dana was born in 1949. Mid-childhood, she moved from the country to central London just as a full-scale blues revival was under way. By 16, she was recording singles for Pye. In 1968, she was signed by Decca, leading to two LPs, the second of which, Box Of Surprises, was written entirely by her. At the turn of the decade, Dana was in the original London cast of two prominent musicals – Catch My Soul and Jesus Christ Superstar. Simultaneously, she was part of a team of session singers (Elton John, David Byron, Madeline Bell) who recorded soundalike versions of pop hits for Avenue Records. She and childhood friend, David Bowie, were both managed by Tony Defries and his MainMan company.
In 1973, Dana's third album, Weren't Born A man, recorded at Trident Studios and produced by Bowie, Mick Ronson, Robin Cable and Dana, came out on RCA. The follow-up, Ain't Gonna Play No Second Fiddle, appeared in 1974 and Dana undertook a US tour in support of it. While Dana was working on demos for her third RCA album, MainMan imploded. Unable to record while still in contract, Dana returned to the UK and sought gigs and acting jobs, appearing in The People That Time Forgot (1977), The Hound Of The Baskervilles (1978), Nic Roeg's Bad Timing (1980) and Mai Zetterling's Scrubbers (1982). Among the people with whom she acted were Doug McClure, Peter Cook, Dudley Moore, Denholm Elliott Joan Greenwood, Terry-Thomas, Kenneth Williams, Roy Kinnear, Penelope Keith, Prunella Scales, Art Garfunkel, Harvey Keitel and Kathy Burke.
In 1982, Dana resumed her recording career with Blue Job (Ace Records), a collection of early 20th century bawdy blues numbers. She kept one foot in the pop camp with the 1984 album, Solid Romance (Bellaphon), which spawned international hit, 'Move Your Body Close To Me', before committing to the blues full-time. She founded and ran the Mustique Blues Festival with Basil Charles OBE, for two decades. In 1997, she joined the UK leg of Bob Dylan's tour as 'special guest'. There are now two strands to Dana's recording career which run in parallel; her singer/songwriter blues albums for Ace Records and (in Europe) Wolf Records, and her albums of bhajans (devotional songs). Dana tours worldwide, including India, Austria, Switzerland, Germany, Britain and beyond, frequently performing with the London Blues Band. She regularly appears in the UK capital at the 606 Club, Chelsea, and The Temple of Art and Music, Elephant & Castle. Her Decca recordings have been anthologised as London Social Degree (RPM, 2019) and her RCA recordings as What Memories We Make (Cherry Red, 2019). Dana's most recent albums are Under My Bed (Ace, 2019) and Deep Pockets (Ace, 2021).
Where do you even start with the task of trying to shoehorn Dana Gillespie's life and experience into a few meagre paragraphs? She is, after all, a singer, songwriter, actress, broadcaster and consummate raconteuse who's lived multiple lives. Although today she's known as the UK's long-reigning Queen of the Blues, Dana has been 108 other things, too. After a happy, unconventional childhood, first in the Home Counties and then in central London, she completed her education with a short spell at the noted Arts Ed stage school. Although she'd had a taste of sporting success as the British Junior Water-Skiing Champion, it was increasingly clear that one calling compelled her beyond all others; music. It was pouring into her teenage life in a number of ways; drumming lessons, guitar, singing, after-school jobs in record shops and, thanks to an ability to make herself look like a grownup, attending gigs at The Marquee in Soho and The Cromwellian Club in South Kensington. It was at the former that she met David Bowie (then Jones) in 1964. The friendship they formed was characterised by the mutual encouragement of each other's incipient songwriting talents. Their paths would converge several times over the ten years that followed.
As Dana's tastes developed, one musical form permanently and profoundly endeared itself to her; the blues. Something about its combination of guts, honesty and libidinous poetry had a transformative effect on her. But, armed with just a twelve-string guitar, Dana knew that her entry into the business of recorded music would be more swiftly effected via another genre that had also enjoyed a full-scale revival in the 1960s; folk. In 1965, signed to Pye Records, Dana issued a series of singles with A-sides by external writers backed by self-composed B-sides. This trio of flip-sides represents the first recorded flourishing of her songwriting talent – 'You're A Heartbreak Man', 'It's No Use Saying If' and 'Adam Can You Beat That'. The accomplishment of composing and recording her own songs at 16 years old placed her in an exclusive group. She and her 60s contemporary, Twinkle, were the first female artists in the UK to write and record their songs while still teenagers. The only other prominent female teens who'd pulled off the feat were in America – Carole King in 1958, Janis Ian in 1965 and Laura Nyro in 1966.
DANA AT DECCA
Dana's growing stature – the singles, live work and appearances on ITV's Ready, Steady, Go – brought her to the attention of Decca Records where she arrived in 1968, joining David Bowie who'd landed at Decca subsidiary, Deram, the previous year. Dana's tenure with the company was remarkably fruitful. First came the pop/folk album Foolish Seasons (1968) which, for reasons still unclear, didn't get beyond test-pressing stage in Britain, but came out on the London imprint in America. Its striking cover photograph was shot by Gered Mankowitz, with whom Dana would enjoy a long creative partnership. Foolish Seasons was primarily written by others, with just a sprinkling of Dana originals. The following year's Box Of Surprises (this time, Decca reversed its baffling strategy, issuing the album in the UK but not in the States) was a portent; all the songs were written by Dana and the album bore traces of her beloved blues, thanks in part to the backing by Savoy Brown. Today, both albums are highly collectable, selling for three-figure sums. At the time of their release they were insufficiently promoted, with just one single creeping out, 'You Just Gotta Know My Mind', a bracingly energetic Donovan original composed especially for Dana and produced by Jimmy Page.
STAGE CAREER
At turn of the decade, Dana joined the cast of Catch My Soul, the rock-musical adaptation of Othello written by its star, Jack Good, and staged at The Roundhouse in London. Among her fellow cast-members were PJ Proby, Marsha Hunt and PP Arnold. But with her recording career in stasis, It was time for a changing of the guard in terms of management. Bowie had just met a potential new manager, Tony Defries. Soon, he and Dana would become part of the Gem music management company where Defries worked with Laurence Myers. Defries quickly secured RCA contracts for his two leading stars. While Bowie got to work on his first RCA LP (Hunky Dory), Dana had other commitments to honour – she was now appearing in the West End, Andrew Lloyd-Webber/Tim Rice blockbuster, Jesus Christ Superstar. Not only was her performance as Mary Magdalene captured on the original London cast soundtrack album (MCA, 1972), she's still, to this day, widely regarded as the best, most convincing Mary, despite stiff competition from Yvonne Elliman and a host of other star performers. Dana would return to the stage in a variety of productions from the 1980s onwards.
MAINMAN, BOWIE, RCA & TONY DEFRIES
By the time Dana's first RCA album, Weren't Born A Man (1973), came out, Defries had launched his own management company, MainMan, which would go down in history for its fearless, radical approach to promotion and its innate, ahead-of-the-times understanding of branding. Weren't Born A Man, 80 per cent Dana's writing, was produced by David Bowie, Mick Ronson, Robin Cable and Dana. Made between 1971 and 1973, it included 'Andy Warhol', written by Bowie for Dana. But the real revelation of the album, apart from the bold, erotic, ruby-hued cover, was the strength of Dana's world-class writing and magisterial singing. It remains a near-perfect example of the thrilling rock/pop/folk/glam hybrid that was being popularised by Dana's peers at the time, including Bowie and Lou Reed. Musicians on the album included Ray Cooper (percussionist from the Elton John Band), Rick Wakeman, Bobby Keyes and arrangers Del Newman and Robert Kirby. The following year's Ain't Gonna Play No Second Fiddle, which turned up the lubricious blues influence a few notches, was another landmark achievement. Second Fiddle was produced by Dana, working with John Porter, who'd overseen Bryan Ferry's These Foolish Things the year before. An uproarious account of the album's wild, bacchanalian sessions was written by engineer Phill Brown in his acclaimed memoir, Are We Still Rolling? (Tape Op, 2010). By now, Dana was living in one of MainMan's apartments in New York. In the States, she promoted her RCA releases with a high-profile national tour, during which Earl Slick and Michael Kamen joined her band when they weren't committed to dates with Bowie.
Had MainMan continued to prosper, Dana might well have had an international breakthrough. Everything was pointing in that direction. But, while work was under way on her third RCA album, MainMan collapsed in a tangle of debts, recriminations and lawsuits. Worse still, until the legalities were unpicked, Dana was stuck in contract and unable to record for anyone else.
Reeling from the blow dealt to her recording career, Dana returned to London, seeking gigs and acting jobs. At the outset of her career, she'd appeared in The Lost Continent (1968) and Ken Russell's Mahler (1974). She resumed film work with The People That Time Forgot (1977), The Hound Of The Baskervilles (1978), Nic Roeg's Bad Timing (1980) and Mai Zetterling's Scrubbers (1982). Among the people with whom she acted were Doug McClure, Peter Cook, Dudley Moore, Denholm Elliott Joan Greenwood, Terry-Thomas, Kenneth Williams, Roy Kinnear, Penelope Keith, Prunella Scales, Art Garfunkel, Harvey Keitel and Kathy Burke.
INTO THE BLUES
Had MainMan not fallen, it's possible that the next stage of Dana's career might not have transpired, and yet it proved to be a vital reboot, enabling her to find most durable musical identity. In the early 1980s, finally free to record, Dana pitched the idea of an album of early 20th-century bawdy blues songs with the punning title of Blue Job. Ace Records eagerly gave their consent, leading not only to a superb album that has remained a key part of Dana's catalogue but also to a long relationship, forty years and counting, with the label. The self-produced Blue Job reintroduced Dana to the album-buying public as one of the UK's most convincing and credible blues stars and even better was to come when she began making blues albums of her own writing. 1984's Below The Belt reunited Dana with her Decca producer, Mike Vernon, and included some of her original blues songs.
Never one for painting herself into a corner, Dana simultaneously tried other things, including modish, synth-based pop music. Her second album of 1984, Solid Romance, issued on Bellaphon and Ariola, spawned a huge European smash with Move Your Body Close To Me, not only written but also produced by Dana. As if to make up for the post-MainMan hiatus, Dana recorded prolifically, with work for Ace, Bellaphon, GiG Records, Wolf Records and more.
DANA DOES DYLAN
In the 1990s, Dana consolidated her European success with a series of blues albums for German and Austrian labels – Where Blue Begins (1991), Blue One (1994), Have I Got Blues For You (1997) and, in collaboration with Joachim Palden, Boogie Woogie Nights (1991) and Big Boy (1992). In 1997, no less a figure than Bob Dylan, with whom Dana had been friendly in the 60s, personally intervened to engage her as the special guest for the UK leg of his 1997 Never Ending Tour. Dana appeared at Bournemouth International Centre, Cardiff Arena, and Wembley Arena. At Wembley, she was joined by Queen's Roger Taylor on drums.
DANA MISCELLANY - WORKING WITH ELTON; RADIO SHOW; MUSTIQUE BLUES FESTIVAL
An indefatigable hunger for experience and experimentation has added scores of additional feathers to Dana's cap. In the late 60s and early 70s, she was an in-demand session singer. While her backing-vocal appearance on Bowie's The Rise And Fall Of Ziggy Stardust And The Spiders From Mars is well-known, she was also part of an elite team of session stars, including Elton John, David Byron (lead singer in Uriah Heep) and Madeline Bell, who lent their uncredited voices to the kitsch, soundalike, pop albums issued by Avenue Recordings. Dana's considerable broadcasting experience includes a world-music radio show, Globetrotting With Gillespie, created for Blue Danube Radio, the Austria-based, international station. The show, syndicated worldwide, ran for over ten years. Dana founded the Mustique Blues Festival, and for two decades ran it with Basil Charles, OBE, in support of his charity which provided scholarships for the children of St Vincent's. Not only did Dana organise acts including Mick Jagger, Ronnie Wood, Ian Siegel and Joe Louis Walker, she produced a fund-raising, Mustique Blues Festival live album for each year she was in charge.
Since becoming a follower of Sri Sathya Sai Baba, Dana has recorded spiritual albums of bhajans (devotional songs) and performed extensively in India. These albums were initially issued using the pseudonym, Third Man, before Dana switched back to using her own name. 1999's Mirrors Of Love album was accompanied by a hardback book of inspirational wisdom compiled by Dana and illustrated by the late Jorg Huber, with whom Dana collaborated for 35 years.
DANA TODAY
Dana's alliance with the London Blues Band has taken her all over the world, while in London she's a regular fixture at the 606 club in Chelsea. Her post-millennial albums have been among the strongest of her career – Experienced (2000), Staying Power (2003), I Rest My Case (2010), Cat's Meow (2014) and capture Dana singing the blues with the kind of conviction and authenticity that can only be acquired with experience. While Dana still writes alone, she has also formed a creative alliance with London Blues Band guitarist, Jake Zaitz, with whom she's composed the bulk of her new material.
In Dana's sixth decade of music-making, it seems that the respect and recognition so long her due is finally coming her way. The last two years have seen a positive critical reappraisal of her work throughout the entertainment press. In 2019, her RCA albums were issued as a deluxe anthology, What Memories We Make (Cherry Red Records), including singles, alternative versions of songs and previously unheard demos for a prospective third MainMan album. Around the same time, RPM Records compiled her Decca work on a collection called London Social Degree. Plans for more archival anthologies are under way. 2019 ended with the release of one of Dana's finest albums of original material, Under My Bed (Ace Records), but promotion was cut short owing to the covid pandemic. Dana's memoir, Weren't Born A Man (Hawksmoor Publishing) arrived in 2021, leading to rapturous coverage in The Times, The Spectator and The Daily Mail.
Since then, Dana has never been busier. She has joined forces with The Temple Of Art And Music in London's Elephant & Castle. Part of the sprawling, street-food emporium, Mercato Metropolitano, the TAM is a new venture in ethical music management and Dana will perform there on a regular basis. It also serves as the principal filming location of Globetrotting With Gillespie, which has just been relaunched as a YouTube show, featuring Dana and some of her favourite figures from the arts, including Marc Almond, Julian Clary, Pandit Dinesh, Big Joe Louis, and Dino Baptiste. Dana's 72nd album, Deep Pockets (Ace Records, 2021), is further evidence that, far from diminishing her gifts, age is only enhancing them. The album, recorded during lockdown in a makeshift studio at the edge of Lake Maggiore, features some of Dana's most searing and perspicacious songwriting, plus blistering performances from her band, made up of members of the London Blues Band and some of Europe's preeminent session musicians.


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So again we have our radio button selected and what I'm going to do is uncheck the display none, so we can see them now. So the approach that we're going to take is to set the element to absolute positioning so that it doesn't disrupt the position of the elements around it, and then we're going to give it a negative left value, which will bump it far to the left but it won't affect horizontal scrolling at all. So let's go ahead and set the position to absolute and then we'll set the left value to a large negative number, let's do negative 999 pixels.


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HOME BROWSE JOURNALS MANCHESTERHIVE MANCHESTER UP
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Eşref Aksu
in The United Nations, intra-state peacekeeping and normative change
Open Access (free)
The United Nations, Intra-State Peacekeeping and Normative Change
New Approaches to Conflict Analysis
Series editor: Peter Lawler, Senior Lecturer in International Relations, Department of Government, University of Manchester
Until recently, the study of conflict and conflict resolution remained comparatively immune to broad developments in social and political theory. When the changing nature and locus of large-scale conflict in the post-Cold War era is also taken into account, the case for a reconsideration of the fundamentals of conflict analysis and conflict resolution becomes all the more stark.
New Approaches to Conflict Analysis promotes the development of new theoretical insights and their application to concrete cases of large-scale conflict, broadly defined. The series intends not to ignore established approaches to conflict analysis and conflict resolution, but to contribute to the reconstruction of the field through a dialogue between orthodoxy and its contemporary critics. Equally, the series reflects the contemporary porosity of intellectual borderlines rather than simply perpetuating rigid boundaries around the study of conflict and peace. New Approaches to Conflict Analysis seeks to uphold the normative commitment of the field's founders yet also recognises that the moral impulse to research is properly part of its subject matter. To these ends, the series is comprised of the highest quality work of scholars drawn from throughout the international academic community, and from a wide range of disciplines within the social sciences.
M. Anne Brown
Human rights and the borders of suffering:
the promotion of human rights in international politics
Karin Fierke
Changing games, changing strategies:
critical investigations in security
Tami Amanda Jacoby and Brent Sasley (eds)
Redefining security in the Middle East
Deiniol Jones
Cosmopolitan mediation?
Conflict resolution and the Oslo Accords
Jan Koehler and Christoph Zürcher (eds)
Potentials of disorder
Helena Lindholm Schulz
Reconstruction of Palestinian nationalism:
between revolution and statehood
David Bruce MacDonald
Balkan holocausts?
Serbian and Croatian victim-centred propaganda and the war in Yugoslavia
Jennifer Milliken
The social construction of the Korean War
Ami Pedahzur
The Israeli response to Jewish extremism and violence:
Tara Väyrynen
Culture and international conflict resolution:
a critical analysis of the work of John Burton
Copyright © Eşref Aksu 2003
The right of Eşref Aksu to be identified as the author of this work has been asserted by her in accordance with the Copyright, Designs and Patents Act 1988.
Published by Manchester University Press
Altrincham Street, Manchester M1 7JA
www.manchesteruniversitypress.co.uk
British Library Cataloguing-in-Publication Data
A catalogue record for this book is available from the British Library
Library of Congress Cataloging-in-Publication Data applied for
ISBN 0 7190 6748 0 hardback
ISBN 9 7818 4779 0910 institutional
by Action Publishing Technology Ltd, Gloucester
https://www.manchesteropenhive.com/abstract/9781526137906/9781526137906.00001.xml
United Nations; peace; security; governance; intra-state conflicts; UN peacekeeping; international community; normative basis; institutionalised values; UN Charter
Series: New Approaches to Conflict Analysis
The United Nations, Intra-State Peacekeeping and Normative Chang
The UN and intra-state conflicts: problematising the normative connection
Rethinking the UN through intra-state peacekeeping: the analytical framework
The UN's role in historical context: impact of structural tensions and thresholds
UN peacekeeping in intra-state conflicts: evolution of the normative basis
The UN in the Congo conflict: ONUC
The UN in the Cyprus conflict: UNFICYP
The UN in the Angola conflict: UNAVEM
The UN in the Cambodia conflict: UNTAC
Reflections on international normative change
Select Bibliography
The United States in the Indo-Pacific
Obama's Legacy and the Trump Transition
Editors: Oliver Turner and Inderjeet Parmar
This edited volume explores the political, economic and security legacies former US President Barack Obama leaves across Asia and the Pacific, following two terms in office between 2009 and 2017. The aim is to advance our understanding of Obama's style, influence and impact by interrogating the nature and contours of US engagement throughout the region, and the footprint he leaves behind. Moreover, it is to inform upon the endurance of, and prospects for, the legacies Obama leaves in a region increasingly reimaged in Washington as the Indo-Pacific. Contributors to the volume examine these questions in early 2019, at around the halfway point of the 2017–2021 Presidency of Donald Trump, as his administration opens a new and potentially divergent chapter of American internationalism. The volume uniquely explores the contours and dimensions of US relations and interactions with key Indo-Pacific states including China, India, Japan, North Korea and Australia; multilateral institutions and organisations such the East Asia Summit and ASEAN; and salient issue areas such as regional security, politics and diplomacy, and the economy. It does so with contributions from high-profile scholars and policy practitioners, including Michael Mastanduno, Bruce Cumings, Maryanne Kelton, Robert Sutter and Sumit Ganguly. The volume will be of interest to students and scholars of the international relations of Asia and the Pacific, broadly defined; US foreign policy and global engagement; the record and legacies of former President Barack Obama; and the foreign policies of the administration of President Donald Trump.
Limiting institutions?
The challenge of Eurasian security governance
Editors: James Sperling, Sean Kay, and S. Victor Papacosma
Eurasian security governance has received increasing attention since 1989. The North Atlantic Treaty Organisation, the institution that best served the security interests of the West in its competition with the Soviet Union, is now relatively ill-equipped resolve the threats emanating from Eurasia to the Atlantic system of security governance. This book investigates the important role played by identity politics in the shaping of the Eurasian security environment. It investigates both the state in post-Soviet Eurasia as the primary site of institutionalisation and the state's concerted international action in the sphere of security. This investigation requires a major caveat: state-centric approaches to security impose analytical costs by obscuring substate and transnational actors and processes. The terrorist attacks on the World Trade Center and the Pentagon marked the maturation of what had been described as the 'new terrorism'. Jervis has argued that the western system of security governance produced a security community that was contingent upon five necessary and sufficient conditions. The United States has made an effort to integrate China, Russia into the Atlantic security system via the Partnership for Peace (PfP) programme and the Euro-Atlantic Partnership Council. The Black Sea Economic Cooperation has become engaged in disseminating security concerns in fields such as environment, energy and economy. If the end of the Cold War left America triumphant, Russia's new geopolitical hand seemed a terrible demotion. Successfully rebalancing the West and building a collaborative system with Russia, China, Europe and America probably requires more wisdom and skill from the world's leaders.
The EU and crisis response
Editors: Roger Mac Ginty, Sandra Pogodda, and Oliver P. Richmond
This is a start-of-the-art consideration of the European Union's crisis response mechanisms. It brings together scholars from a range of disciplinary backgrounds to examine how and why the EU responds to crises on its borders and further afield. The work is based on extensive fieldwork in among another places, Afghanistan, Libya, Mali and Iraq.
The book considers the construction of crises and how some issues are deemed crises and others not. A major finding from this comparative study is that EU crisis response interventions have been placing increasing emphasis on security and stabilisation and less emphasis on human rights and democratisation. This changes – quite fundamentally – the EU's stance as an international actor and leads to questions about the nature of the EU and how it perceives itself and is perceived by others.
The volume is able to bring together scholars from EU Studies and Peace and Conflict Studies. The result showcases concept and theory-building alongside case study research.


This plant based candle is hand poured in England combining fine quality oils and natural wax.
Presented in a recycled glass jar with a lid, this candle will burn cleanly for approximately 50 hours.
Perfect for when you need a little mood boost.


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Recent Top Ten Specific Date Date Range By Official By Location By Topic
China Vitae tracks the appearances and travel of up to 200 leading Chinese officials. Information is available on the date and location of the activity, officials in attendance, the topics raised, and the source of the data.
For a complete record of official's appearances and travel, click on the official's name.
China Vitae uses the following sources to obtain appearance and travel information on tracked officials: (View Sources)
VIP Appearances & Travel
Activity: Jiang Zemin attended the concert to commemorate the 110th anniversary of the birth of the late Chairman Mao Zedong.
Location: Beijing Municipality
Attendees: Jiang Zemin, Chen Zhili, Hui Liangyu, Li Changchun, Liu Qi, Liu Yunshan, Sun Jiazheng, Wang Gang, Wen Jiabao, Wu Guanzheng, Xu Caihou, Zeng Qinghong
Topics: domestic politics
December 26, 2003 (Jiang Zemin)


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HomeFeatured WalksHow we grade walking routes on Walking Tenerife
How we grade walking routes on Walking Tenerife
October 31, 2018 Jack Featured Walks, Walking Tips 0
Anyone who has spent any time on specialist walking websites will already know one sure thing when it comes to how walking routes are graded – it varies enormously. It's the same when it comes to how different countries grade their own official walking routes.
In order to try to improve our own way of doing it we researched how it was done elsewhere… and found it was a minefield. We wrote an article about these findings on our Buzz Trips website which specialises in hiking and dining around the world.
One of the higher parts of Fuerteventura.
There's a very good reason for the variations in how routes are graded – locations vary not only in topographical features but also in the quality of paths. For example, even in the Canary Islands walking on Fuerteventura is a quite different experience from walking on La Gomera in terms of the terrain you encounter and the ascents/descents involved.
And the view from a path in Teide National Park.
In some countries official paths can be wide and well maintained. Many we've walked in Germany have been like this. In other places paths aren't maintained at all. We've found this in parts of Greece and Portugal. The state of paths can impact on difficulty levels almost as much as distance and ascents/descents involved. Take the Masca Barranco on Tenerife. It's not a long walk from the hamlet to the coast, but it is a challenging one thanks to the terrain. There's a lot of scrabbling involved which requires a certain level of dexterity. We've been banging the drum about Masca being a challenging route for years whilst some others strongly disagreed.
Tellingly, Masca Barranco remains closed to the public because of the amount of accidents that have occurred on the route.
Uneven path in the Masca Barranco.
Up until now we've generally applied a more descriptive way of explaining the difficulty level of the routes in our walking sets. This involves a general outline of the differences between a level 1 route (more a long stroll than a hike) and a level 5 route (only recommended for experienced and fit hikers).
Easy coastal walking.
On each of the routes in our walking sets we go into more detail, taking into account the terrain as well as distance and often total ascent/descent. For each area of Tenerife we try to balance out routes in each package so there are ones which are accessible to most reasonably fit walkers and a route which offers more of a challenge. However, we're not into hardcore hiking so none are like the routes you might encounter in places like Corsica which are used to train the French Foreign Legion. We view walking as a fun activity.
And a challenging descent to the coast.
However, people have occasionally asked what the route grading means on the descriptions on our Buy Islands Walks page as they're a bit on the vague side.
It's a fair question, so here's a more detailed explanation of how we grade our routes:
Level 1: Under 5km and with ascents/descents which are 100m or less.
Level 2: Under 10km with ascents/descents which are under 200m.
Level 3: Between 10 and 15km with ascents/descents up to 500m.
Level 4: Between 10 and 15km with ascents/descents up to 1000m.
Level 5: Ascents/descents of over 1000m.
Not as challenging as it might look.
Not everything in nature fits neatly into boxes, so there are a couple of variations depending on individual route characteristics. But generally speaking these should give anyone who's interested a better idea of what is involved.
descents
difficult routes
easy routes
grades of walking routes
hiking routes
Walking route from Tenerife's north coast to Mount Teide
Walking through the old quarter in La Laguna
Six scenes of the Teno Alto walking route on Tenerife
August 22, 2018 Jack 0
There are a handful of walking routes on Tenerife which instantly would spring to mind if we wanted to impress visiting friends who enjoyed a jaunt in the countryside. Teno Alto in the west of the island one of them. […]
Tenerife Government Bans Fires on Tenerife Due to Hot Weather Warning
July 9, 2010 Jack 2
The Tenerife Cabildo has issued a ban on agricultural burning, bonfires and fires of any kind in the countryside from Thursday 8 July until the end of the hot weather alert for Tenerife. No fires […]
Tenerife Hiking, Discover the South West
July 27, 2010 Andy 4
For many, the south west coast of Tenerife represents the perfect holiday destination, enjoying as it does some of the longest sunshine hours on the island. […]


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Learn to Speak Hungarian
The history of the language originated around 1000 BC. The Hungarians call their language Magyar. For the most part the Hungarian language stems from Asia (the Uralic region). The language has also been influenced by various Slavonic languages, as well as by the German language.
Hungarian is part of the Ugrian subgroup of Uralic languages, and so is not part of the Indo-European family tree. Its nearest 'relatives' are Finnish and Estonian.
Learn Hungarian Today!
Learn Hungarian with Language Door Today!
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Facts About the Hungarian Language
The name Hungary or Hungarian is believed to come from the semi-nomadic tribe called the Huns that lived throughout central and southeastern Europe, most well-known is Attila the Hun in ~500 AD and some Huns had settled for a brief time in Hungary.
Hungary has borders with seven countries in Europe, starting directly north and moving clockwise is Slovakia, Ukraine, Romania, Serbia, Croatia, Slovenia and Austria.
Hungarian uses the Roman alphabet.
The Hungarian language has 25 consonant phonemes and 14 vowel phonemes.
When speaking the language, the primary stress is always on the first syllable of a word.
Where Is Hungarian Spoken?
There are about 14 million native speakers of the Hungarian language. Most live in Hungary (about 9.5-10 million), about 1.4 million in Romania, about 2.5 million mainly in Transylvania, but also Slovenia, Czech Republic, Ukraine, Croatia, Slovakia, Serbia, and Austria. There are also many people of Hungarian origin in the UK and other European countries, the USA, Canada and Australia.


Crusher Dust is a crushed blue metal dust. It is approximately 8mm slithers down to dust and is blue grey in colour. You can stabilise this by mixing cement with it, this will make it more durable. Crusher Dust can also be used to put under water tanks, and concrete slabs or to backfill trenches.
2013-11-15· Hi All I have put down a 50mm crusher dust path/hardstand area (stabilised with cement) around my garden shed. I'm wondering whether you know of any product that will help bind the top surface (without applying say an asphalt surface etc.).
cotton soil, the yellow clay, and the red murrum mixed with stone dust (crusher dust) in ... stabilization of the soils by mixing good soil with the poor, ... either the sand or the crusher dust (stone dust) or both could ... and grading using the concept of suitably index. ... From Table 1 it can be seen that the black cotton soil and the.
Specially designed mixes to bring different qualities of gravels together. Also offering stabilised gravels and washout fill as required.
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2011-10-02· Compacted crusher dust or roadbase is perfectly adequate for a perimeter path – and if you mix cement in with it (14x20kg bags per cubic metre of roadbase/crusher dust makes a pretty good "concrete") and thoroughly compact it, it will draw in ground moisture and set hard anyway. A thin layer of decorative gravel (like rhyolite) over the top and you're done.
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Southern Quarries produces quarry sands to Department of Planning, Transport and Infrastructure specifications. Southern Quarries is a South Australian market leader in the production of manufactured sand specifically designed for use in the production of concrete.


text:
Die fromme Helene ist eine Bildergeschichte des satirischen Zeichners und Dichters Wilhelm Busch. Die Geschichte erschien 1872 im Bassermann Verlag, der von Wilhelm Buschs langjährigem Bekannten Otto Friedrich Bassermann geleitet wurde. Die Geschichte ist ähnlich wie Buschs Bildergeschichte Der heilige Antonius von Padua von Buschs antiklerikaler Haltung geprägt. Vor dem Hintergrund des Kulturkampfes in Deutschland fand die Bildergeschichte schnell eine große Leserschaft. Sie zählt auch noch heute zu den populärsten Geschichten Wilhelm Buschs.

Inhalt und Hintergrund
In der frommen Helene beleuchtet Wilhelm Busch satirisch religiöse Heuchelei und zwielichtige Bürgermoral:
Ein guter Mensch gibt gerne acht,
Ob auch der andre was Böses macht;
Und strebt durch häufige Belehrung
Nach seiner Beß'rung und Bekehrung

Viele Einzelheiten der frommen Helene sind von biographischem Erleben Buschs geprägt. 1867 lernte er durch seinen Bruder Otto Busch die Frankfurter Bankiersgattin Johanna Keßler kennen. Zwischen den beiden kam es zu einer mehrjährigen Freundschaft, die nach der Einschätzung der überwiegenden Zahl der Busch-Biographen aber platonisch blieb. Die siebenfache Mutter war eine einflussreiche Kunst- und Musikmäzenin in Frankfurt und glaubte in Busch einen bislang noch nicht hinreichend gewürdigten Maler zu entdecken. In dieser Einschätzung wurde sie von Anton Burger, einem führenden Mitglied der Kronberger Malerkolonie gestützt. Johanna Keßler richtete in ihrer großzügigen Villa an der Bockenheimer Landstraße Wilhelm Busch Wohnung und Atelier ein. Motiviert von ihrer Unterstützung und Bewunderung widmete Wilhelm Busch sich in seinen Frankfurter Jahren vor allem der Malerei, verbrachte viel Zeit im Städel und war als Maler sehr produktiv. Johanna Keßler stand seinem humoristischen Werk dagegen kritisch gegenüber. Die fromme Helene entstand in der Spätphase von Buschs Frankfurter Jahren und weist Elemente einer zunehmenden Entfremdung und kritischen Auseinandersetzung mit dem Lebenskonzept der Familie Keßler auf.

Johanna Keßler war mit einem deutlich älteren Mann verheiratet und ließ ihre Kinder von Gouvernanten und Hauslehrern großziehen, während sie eine aktive Rolle im Frankfurter Gesellschaftsleben spielte.
Schweigen will ich vom Theater
Wie von da, des Abends spät,
Schöne Mutter, alter Vater
Arm in Arm nach Hause geht.

Zwar man zeuget viele Kinder,
Doch man denket nichts dabei.
Und die Kinder werden Sünder,
Wenn's den Eltern einerlei.
Auch die Heirat der deutlich gealterten Helene mit dem reichen G. I. C. Schmöck scheint eine Parallele zu Johanna Keßlers Mann Johann Daniel Heinrich zu sein, der seinen Namen zu J. D. H. Keßler abkürzte. Nach Meinung der Busch-Biographin Eva Weissweiler leitet sich Schmöck von Schmock ab, einem jiddischen Schimpfwort, das Dummkopf, Trottel oder Idiot bedeutet. Johanna Keßler wird auch diese Anspielung verstanden haben, denn ihr Mann war an Kunst und Kultur gänzlich uninteressiert.

Im zweiten Teil der frommen Helene greift Wilhelm Busch das katholische Pilgerwesen an. Die fromme Helene erschien zur Zeit des Kulturkampfes und Wilhelm Busch hatte bereits mit dem heiligen Antonius von Padua eine große antikatholische Leserschaft gefunden. Allerdings kann man davon ausgehen, dass Wilhelm Busch nicht gezielt antikatholische Tendenzen ausnutzte, um eine breite Leserschaft zu finden. Der protestantisch erzogene Wilhelm Busch bezweifelte auf Grund seines pessimistischen Menschenbilds von vornherein das katholische Heiligenwesen. In satirischer Weise überzeichnet er ein ihm fremdes Lebensgefühl. Auch dies hat einen biografischen Hintergrund. In seiner Münchner Zeit hatte er gemeinsam mit Münchner Künstlerkollegen Kloster Andechs aufgesucht und belustigt festgestellt, wie viele Pilgerpärchen sich im Umfeld des Klosters zu Schäferstündchen ins Gebüsch zurückzogen.

Begleitet von ihrem Vetter Franz geht die bislang kinderlose Helene auf Wallfahrt. Die Wallfahrt zeigt Erfolg; nach gebührender Zeit bringt Helene Zwillinge zur Welt, deren Ähnlichkeit mit ihrem Erzeuger jedoch dem Leser deutlich macht, dass nicht Schmöck, sondern Vetter Franz der Vater ist. Schmöck erstickt in seiner frischen Vaterfreude an dem Festmahl, das er sich zu Gemüte führt, und Vetter Franz findet auch ein vorzeitiges Ende, da er sich nicht nur für Helene, sondern auch für das Küchenpersonal interessiert und deshalb vom eifersüchtigen Kammerdiener Jean erschlagen wird. Der nun verwitweten Helene bleiben nur Rosenkranz, Gebetbuch und Alkohol als Lebenstrost. Ihr Ende findet sie, als sie betrunken in eine brennende Petroleumlampe stürzt; ihre Seele wird daraufhin vom Teufel selbst in die Hölle entführt, wo sie dem bereits eingetroffenen Franz Gesellschaft leisten muss.

Nach Helenes tragikomischem Ende formuliert der Spießer Nolte einen Moralsatz, der vielfach als treffende Zusammenfassung schopenhauerischer Weisheit ausgelegt wird:
Das Gute – dieser Satz steht fest –
Ist stets das Böse, was man läßt!

Verfilmung
Die fromme Helene wurde 1965 von Axel von Ambesser unter gleichem Titel verfilmt. Die Titelrolle spielte Simone Rethel.

Weblinks

 Die fromme Helene bei zeno.org

Ausgaben
 Wilhelm Busch: Die fromme Helene. 10. Auflage, Diogenes, Zürich 2007, ISBN 978-3-257-20109-3 (in Zusammenarbeit mit Wilhelm-Busch-Gesellschaft Hannover).

Literatur
 Michaela Diers: Wilhelm Busch, Leben und Werk. dtv, München 2008, ISBN 978-3-423-34452-4.
 Joseph Kraus: Wilhelm Busch. Rowohlt, Reinbek bei Hamburg 1970; 16. Auflage 2004, ISBN 3-499-50163-5.
 Gudrun Schury: Ich wollt, ich wär ein Eskimo. Das Leben des Wilhelm Busch. Biographie. Aufbau, Berlin 2007, ISBN 978-3-351-02653-0.
 Gert Ueding: Wilhelm Busch. Das 19. Jahrhundert en miniature; Insel, Frankfurt am Main 1977; Neuauflage 2007, ISBN 978-3-458-17381-6.
 Eva Weissweiler: Wilhelm Busch. Der lachende Pessimist. Eine Biographie. Kiepenheuer & Witsch, Köln 2007, ISBN 978-3-462-03930-6.

Einzelnachweise

Literarische Figur
Literarisches Werk
Literatur (19. Jahrhundert)
Religionsparodie
Deutscher Comic (Werk)
Bilderbuch
Werk von Wilhelm Busch


Listen to the Newest Episode of the Soaps In Depth Podcast!
In this week's episode, Charlie and Richard dive into how the change in headwriters over at THE YOUNG & THE RESTLESS will impact the future of Genoa City as the soap moves forward. Plus, with Valentine's Day coming up, they celebrate with a look at your favorite couples. And there's also a peek into the mailbag to see what the listeners are buzzing about right now!
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For more news on all your favorite daytime dramas, keep reading Soaps In Depth magazine.
Y&R Snags GH Actress Brytni Sarpy for a New Role!


"They" say big things always happen in threes (or is that bad things?).
1) The Sonics traded franchise star Ray Allen and drafted Kevin Durant.
2) Mike Hargrove shocked everyone when he stepped down as the Mariners manager and was replaced, as of today, by John McLaren.
3) The Sonics are expected to hire a new coach this week, perhaps as soon as Monday.
So much for a sleepy summer. Wonder if we're done or if a summer of turmoil has just begun.
Perhaps the Mariners, who with or without Hargrove are feeling more and more like contenders, will make a big move.
Over at USS Mariner, they speculate on what it might take to get the White Sox to dish pitcher Mark Buehrle to Seattle. Most interesting part of the analysis is how they challenge the notion that trading prospects, such as Wladimir Balentien or Jeff Clement, for Buehrle doesn't mortgage the future.
I grew up in Atlanta and as a tyke sometimes watched pro wresting on what was then WTBS. My buddies and I sometimes went as far as to quote the Nature Boy Ric Flair (only Ali was better at being arrogant). So maybe my interest in the AJC's story on Lex Luger is just a product of that. Still, on the heels of the murder-suicide involving Chris Benoit, this is a good followup.
Finally, if you A) dislike A-Rod; 2) Love New York tabloid journalism, this is for you.


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text:
The voice of the Republic's forgotten
Annette Joseph-Gabriel
The film BACK UP! and important conversations about state violence, racism, global imperialism, and, crucially, the internal workings of social movements.
Still from "BACK UP!"
For observers of the endless examples of racism in the United States legal system, the month of October began with surprising news. Amber Guyger, the white Dallas police officer who shot and killed Botham Jean, her black neighbor, in his living room, was found guilty of murder. Across the Atlantic, on the same day that Guyger was convicted in Dallas, Assa Traoré, the family member of another slain black man, who died in 2016 in police custody under suspicious circumstances, stood in a French courtroom. Unlike Guyger, there were no hugs or tears for Traoré. Instead, Traoré was the defendant in a defamation suit for naming on social media the three police officers involved in her brother's arrest on his 24th birthday, the arrest that led to Adama's death in a holding cell.
Still in France, but some 6,000 kilometers and an ocean away from Paris, police brutality is not a particularly hot button issue. Instead, in Martinique, the long-running scandal is of the poisoning of agricultural land and water sources with the American-manufactured insecticide chlordecone, nearly 20 years after the French government banned its use in the metropole. The fact that the French state did not take the same measures to safeguard the health of its citizens in the Caribbean, allowing the continued use of a chemical deemed too toxic for the rest of France, means that the chlordecone scandal, like Adama Traoré's death, is very much about French racial discrimination that has its roots in the country's imperial past.
With the US as the site of production, France as the seat of power, and Martinique on the receiving end of destructive policies driven by economic expediency, chlordecone unites three disparate places that are rarely spoken of in the same breath. Indeed, not many people connect police brutality in the French capital to an environmental disaster that has given the small Caribbean island the dubious distinction of having the highest rate of prostate cancer in the world. Connecting those dots is at the heart of the new film BACK UP! by Christophe Gros-Dubois.
BACK UP! is a low-budget film that makes lofty promises, and it delivers on some of them. The story revolves around a group of underground investigative journalists who style themselves "the voice of the Republic's forgotten." Their mission is to uncover the hidden truth behind acts of state violence. At the start of the film, that violence comes in the form of the police killing of Sami Adibi, a young French man of Tunisian origin. The police have no answers, only the well-rehearsed narratives that criminalize the dead man.
Still from "BACK UP!"
The four journalists of Back Up turn to social media to diffuse their counternarrative. Professor X pontificates on camera. Hakim Tafer, the ostensible leader, meets with an American investor who can "take Back Up to the next level." The group's only white journalist bears that most quintessential of French names, François, and sees his work behind the camera as a steppingstone to a more stable gig at a mainstream media outlet. The only woman, La Rageuse, is also the only person who does any investigating. Her task is to connect Adibi's death to historical examples of state violence, specifically the disappearance of Georges Beltran, a Martinican activist in Paris, in the 1990s. Amidst a flurry of likes, livestreamed chats and debates about how to maintain a steady flow of faithful online followers, the conflict among the underground journalists reveals the complicated mess that the neat descriptor of "speaking truth to power" seeks to encapsulate.
Expertly played by Astrid Bayiha, La Rageuse is at once the film's flaw and redeeming quality. Hers is a compelling character. She is angry. We know that it is a justified, righteous anger. But we do not know the reason for it. In fact, we never learn her real name. For all its self-reflexiveness on the fine line between the radical politics of sousveillance and the self-aggrandizing desires of activist-influencers, the film is remarkably shortsighted in its rendering of La Rageuse as yet another angry black woman with no backstory.
Despite the film's shortsighted representation, La Rageuse is also the only one who sees that Back Up's insistence on access to the Adibi family's grief "for the cause" borders on the exploitative. She questions the ways that the underground nature of the group's work will be changed by American investment backing. In a film peppered with heavy-handed monologues about the illegitimacy of state violence, La Rageuse is the voice of nuance. On one occasion when a security guard ejects her from the grounds of a company she is investigating, she throws a tampon in his face as a metaphorical middle finger. Ultimately, she is the only person who connects Martinique's white plantation owners and French police as complementary agents of state violence via economic repression and brutal force respectively.
Police brutality and the chlordecone scandal come together in the film as the Janus-faced representation of the French state, terrifying on both sides. The shaky movements of the handheld camera simulate the clandestine smartphone recordings that the Back Up team marshal as evidence in the court of public opinion. They invite the viewer to consider the promises and limits of body cameras and bystander footage as tools for destabilizing state power. Juxtaposed with the grainy images of the VHS tape of the disappeared Martinican activist, Georges Beltran, they also invite the viewer to connect past to present, environmental crimes in the French Caribbean to racism in the French capital.
BACK UP! offers no answers, even as it clumsily attempts a neat resolution at the end of the film. But it is the start of an important conversation about the global net cast by imperialism then and now.
For observers of the endless examples of racism in the United States legal system, the month
Africa doesn't care about its women
Seun Babalola
I have the privilege to fight, argue or board a plane when I feel like I've had enough. The vast majority of women on the continent do not have that option.
African poets get their own documentary
The friendship of the poets Syl Cheney-Coker and Niyi Osundare is the subject of the road movie documentary, "The Poets."
Intimacy in Africa, on film
Chandani Patel
Bringing attention to African filmmakers who challenge prevalent cinematic depictions of the continent.
"This is not Pantsula"
Bukola Afolayan
I got a treat when I was in Johannesburg recently. I was about to jump into a cab when this van pulls up and out piled these colorfully clad kids. With their exit came the …


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text:
Q: Why is pressing back removing all the navigation bar information whilst in landscape? I've not been able to find anyone speaking of the same problem but i'm quite happy to be pointed to a post where something like this has been resolved.
As it stands, in portrait.
Picture 1: shows a media list, you can click a piece of media

Picture 2: you press the back arrow "<"

Picture 3: It returns you back to the correct page with everything working

Now, when I attempt to do this with a landscape orientation (locked or unlocked).
As you can see, "View Media" at the top has disappeared and so has the Logout button.

Some things i've noticed about the problem:

 After carrying out that second sequence in landscape mode, if I rotate to portrait all the features return. I've also noticed that while it's animating from the View Media page to the piece of media, if I click back ("< View Media") while the black bar is coming across (picture below) it all is there when I go back.

I've got nothing in the code that affects the orientation anymore as I thought i'd remove it all to start debugging but i've tried quite a few things and I think i'm barking up the wrong tree. Anyone pointing me in the right direction would be helpful! Thank you

A: I found the problem but I wouldn't really call it an answer. I've got the status bar hidden on the second VC in landscape but when I show it everything resumes fully working.. If I want to hide the status bar i'm having to create a fake back button:
UIBarButtonItem *item = [[UIBarButtonItem alloc] initWithTitle:@"Back" style:UIBarButtonItemStylePlain target:self action:@selector(popVC)];
self.navigationItem.hidesBackButton = YES;
self.navigationItem.leftBarButtonItem = item;

then in popVC just
[self.navigationController popViewControllerAnimated:YES];


China's Ministry of Railways and its associated companies have a mountain of debt (see 10 reasons to short China), quite simply because of the over-aggressive expansion of the high-speed rail. Now, the ministry's disclosure confirms that the Ministry and its associated companies have lost RMB3.76 billion in the first quarter of 2011. Despite earlier rumour that the ministry will cut investment plans, the ministry denies it.
The representatives of the ministry said the main cause of the loss was increasing prices of diesel, steel and other parts and materials. Previously some suggested that the ministry will cut investment by RMB200 billion, yet the ministry denied it. The latest disclosure also shows that the ministry has RMB1.983 trillion of debt, slightly higher than the RMB1.8 trillion that we have already known, while the total asset amounts to RMB3.4 trillion.
This is hardly surprising. The infrastructure expansion in China was obviously too aggressive, and the problems with high-speed rail is that it is too expensive for most people, so it is destined to be under-utilised, so it is unclear if the ministry can make enough money in the long-run to service and repay its debt. On a more positive side, it is a good thing that the ministry disclose some numbers to the public, even those numbers were hardly reassuring.


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text:
Чарльз Джозеф Керстен (Charles Joseph Kersten; 26 травня 1902 — 31 жовтня 1972) — республіканець, антикомуніст, представник Вісконсину в конгресі США в 1947-49 і 1951 — 55 рр.

Увагу до історії України почав звертати після вбивства С. Петлюри. Керстену належать слова:
«Московські методи нищення українців такі немилосердні, що їх в історії тиранії ніде знайти не можна.»

Примітки

Посилання
Керстен Чарльс  //
 Історія ненависті  // форум на censor.net

Республіканці США
Члени Палати представників від штату Вісконсин
Американські антикомуністи
Адвокати США
Римокатолики США


text:
Angiogenesis is regulated by indicators received by receptor tyrosine kinases such
Victoria Harper Main August 28, 2018 224452-66-8 IC50, Lum
Angiogenesis is regulated by indicators received by receptor tyrosine kinases such as for example vascular endothelial development factor receptors. Traditional western blot evaluation, and immunofluorescent histochemistry. Spry2 mRNA and proteins amounts in the wound bed more than doubled through the resolving stages of curing, coincident using the starting point of vascular regression with this wound model. In another test, intracellular degrees of Spry2 or its dominant-negative mutant (Y55F) had been raised with a topical ointment application towards the wounds of controlled-release gel comprising cell permeable, transactivator of transcription-tagged Spry2, Spry2Y55F, or green fluorescent proteins (as control). Wound examples had been analyzed for vascularity using Compact disc31 immunofluorescent histochemistry aswell for total and phospho-Erk1/2 proteins content. The treating wounds with Spry2 led to a significant reduction in vascularity and a lower life expectancy great quantity of phospho-Erk1/2 weighed against wounds treated using the green fluorescent proteins control. On the other hand, the wounds treated using the dominant-negative Spry2Y55F exhibited a moderate upsurge in vascularity and raised phospho-Erk1/2 content material. These outcomes indicate that endogenous Spry2 features to downregulate Lum angiogenesis in the curing murine pores and skin wound, possibly 224452-66-8 IC50 by inhibiting the mitogen-activated proteins kinase signaling pathway. period stage, and 3-mm punch-biopsy tools had been employed for wound harvesting during afterwards time points; hence, all gathered wound examples from the many time points were left with around the same quantity of encircling unwounded tissue in accordance with the wound region. The lower of your skin with 224452-66-8 IC50 its exclusive postwounding revascularization design aswell as the current presence of a scar tissue (seen as a having less locks) are two strategies that were employed for the id from the wound region during afterwards time 224452-66-8 IC50 points, specifically on and postwounding. The mice had been housed in sets of five at 22 to 24C on the 12-h:12-h light-dark routine; water and food had been provided advertisement libitum. Pet protocols found in these research had been reviewed and accepted by the Institutional Pet Care and Make use of Committee from the School of Illinois at Chicago. All pet procedures had been conducted relative to the (Country wide Institutes of Wellness). Total RNA Removal and Real-Time PCR Wound examples kept in RNAlater alternative (Sigma) had been homogenized in TRIzol reagent (Invitrogen, Carlsbad, CA). Total RNA was isolated and treated with DNase I based on the Invitrogen process and examined for purity, and its own focus was quantified spectrophotometrically. Total RNA (1 g) was invert transcribed to cDNA using the RETROscript RT package (Invitrogen). GAPDH primers had been released previously (20). Spry2 primers had been designed using SciTools PrimerQuest software program (Integrated DNA Technology, Coralville, IA); Spry2 primer sequences had been the following: forwards, 5-ACTGCTCCAATGACGATGAGGACA-3; and invert, 5-CCTGGCACAATTTAAGGCAACCCT-3. cDNA examples, upstream and downstream primers for both endogenous control gene (GAPDH) and the mark gene (Spry2), and SYBR Green PCR Professional Combine (Applied Biosystems, Foster Town, CA) had been packed onto MicroAmp 96-well PCR response plates (Applied Biosystems), as well as the amplification process was operate using the ABI Prism 7000 and StepOnePlus Real-Time PCR systems (Applied Biosystems). Fresh threshold routine (Ct) data had been analyzed using the Ct technique, as previously defined (18). Values produced for each test are normalized to GAPDH at every time stage, and the info are portrayed as fold boosts in gene appearance relative to regular, unwounded skin. Comparative RNA appearance was put through statistical evaluation by one-way ANOVA and Bonferroni's posttests using GraphPad Prism 4.0 software program (GraphPad Software, NORTH PARK, CA). Proteins Extraction and Traditional western Blot Evaluation Wound examples (3 mm) that were kept iced at ?80C were homogenized in 500 l of radioimmunoprecipitation assay buffer using a protease inhibitor cocktail (1/100 dilution; Sigma). Examples had been centrifuged at 13,000 rpm at 4C for 15 min. The causing supernatants had been collected, as well as the proteins concentrations had been quantified utilizing a BCA proteins assay package (Pierce, Rockford, IL). Proteins extracts had been blended with SDS-PAGE buffer and 2-mercaptoethanol (5% of total) and boiled for 3 min. Proteins examples (30C55 g per street) had been loaded into related wells inside a 10% Tris-glycine acrylamide gel (Bio-Rad, Hercules, CA). Separated protein had been used in a nitrocellulose membrane and clogged with 5% skim dairy in Tris-buffered saline. Antibodies had been put on the membrane for 1 h and cleaned with Tris-buffered saline-Tween 20. For the analysis for Spry2 existence, rabbit anti-human Spry2 224452-66-8 IC50 (1/500; Sigma) was utilized. For the analysis for MAPK signaling protein, rabbit anti-rat p44/42 MAPK 224452-66-8 IC50 [total (t)-Erk1/2; Cell Signaling Technology, Danvers, MA] or rabbit.
Four different monoclonal antibodies (mAbs) reactive with rat CD11b (ED7, ED8,
Victoria Harper Transferases June 10, 2017 Evofosfamide, Lum
Four different monoclonal antibodies (mAbs) reactive with rat CD11b (ED7, ED8, OX-42 and 1B6c) have already been characterized for their ability to induce homotypic aggregation of granulocytes or to modify granulocyte adhesiveness triggered by phorbol myristate acetate (PMA) or (IgG1). serum (FCS). Purity of cells was >95% granulocytes. For cross-blocking experiments granulocytes were elicited from the peritoneal cavity 4 hr after thioglycollate i.p. injection. Cells were washed twice with phosphate-buffered saline (PBS; pH 74) containing 01% bovine serum albumin (BSA). Cross-blocking experimentsCross-blocking of anti-CD11b mAbs was determined by preincubating granulocytes (1106 cells/ml) with either ED7, ED8, OX-42, 1B6c or control mAb (20 g/ml) 20 min at 4 after which biotinylated ED7, ED8 or OX-42 mAbs (25 Evofosfamide g/ml) were added and incubated for another 30 min at 4. Cells were washed once with PBS and binding of the biotinylated mAbs was detected with avidinCphycoerythrin (PE) (Vector, Burlingame, CA) using a fluorescence-activated cell sorter (FACScan; cytometer (Becton Dickinson, Oxford, UK). Aggregation assaysIn order to measure homotypic aggregation of granulocytes semiquantitative and quantitative aggregation assays were used. For the semiquantitative aggregation assay cells were resuspended in RPMI-1640 with 5% FCS at 5106/ml (5105/per well) and placed in 96-well flat-bottom microtitre plates, incubated at 37 in the absence or presence of mAbs and/or PMA and fMLP. Where indicated, the cells were preincubated with mAbs (at 4) or metabolic inhibitors (37) for 30 min. The following concentrations of inhibitors were used: sodium azide (02%); EDTA (004%); cytochalasin B (5 g/ml); genistein (50 g/ml); staurosporine (05 m); okadaic acid (1 m); orthovanadate (100 m); H7 (80 m); HA1004 (10 m) and bisindolylmaleimide (1 m). Semiquantitative scoring of aggregation was carried out after various time periods as described.22 Scores ranged from 0 to 5: 0. no aggregation; 1. less then 10% of cells in aggregates; 2. 10C50% of cells formed small clusters; 3. 50C80% of cells in small loose and/or compact clusters; 4. >80% of cells formed large loose and/or compact clusters; 5. nearly 100% of cells in large very compact aggregates. All plates were scored by two independent observers. For the quantitative aggregation assay cells were resuspended in Evofosfamide RPMI-1640 with 5% FCS at 2,5106/ml, placed in a Terasaki microwell plate (in Evofosfamide 20 l) and cultured in a hanging drop at 37 for 2 hr. Lum Where indicated, granulocytes had been preincubated with mAbs or metabolic inhibitors as referred to above. Thereafter, the amount of free of charge cells had been counted inside a haemocytometer and percentage of cells in aggregates was dependant on the following formula: Percent of cells in aggregates=100[1?(amount of free of charge cells following cultivation)/(amount of free of charge cells in moderate control before cultivation)]. Colorimetric assay for granulocyte adherence to plasticWe utilized a revised assay, referred to by Oez et al initially.23 Quickly, cells had been resuspended in RPMI-1640 with 5% FCS, preincubated with mAbs at 4 for 30 min and seeded at 5105/per well in 96-well flat-bottom microtitre plates in the existence or lack of PMA or fMLP. The plates had been incubated at 37 for 1 hr. Following this, non-adherent cells had been removed by cleaning 3 x with saline and staying adherent cells had been stained with 01% methylen blue in PBS for 15 min. The plates had been washed 3 x in running drinking water and remaining to air dried out. Evofosfamide Thereafter, 200 l 01 n HCL was put into each well and absorbance of dissolved dye was assessed by an enzyme-linked immunosorbent assay (ELISA) audience (Behring ELISA Processor chip, Behring, FRG) at 650 nm. Outcomes Different epitopes for the rat Compact disc11b molecule identified by mAbs The 1st goal of this research was to define epitope specificity of four mAbs which understand the -subunit of rat CR3 (Compact disc11b). The outcomes of cross-blocking tests presented in Desk 1 display that preincubation of granulocytes with both ED7 and ED8 led to almost full cross-blocking of.