text:
CULTURE APPARATUS
The invention provides a culture apparatus capable of setting preferable environments for each element of a small-sized culture chamber and manipulator, allowing an automatic culturing operation. The culture apparatus according to the invention is provided with a first chamber and a second chamber being a culture chamber. A device for producing moisture is arranged only within the second chamber. Accordingly, a difference between the humidity environment of the first chamber and that of the second chamber can be produced, and thus a manipulator arranged outside the second chamber, accessible to the second chamber can be prevented from being adversely affected by moisture.
Kan, Kazutoshi (Kawagoe, JP)
Kobayashi, Toyoshige (Hatoyama, JP)
435/286.5
435/286.1, 435/303.1
C12M1/38; C12M1/36; C12M3/00
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BOWERS, NATHAN ANDREW
MATTINGLY & MALUR, PC (ALEXANDRIA, VA, US)
1. A culture apparatus that cultures cells using a culture vessel, the culture apparatus comprising: a first chamber; a device controlling a temperature within the first chamber; a second chamber housed in the first chamber, having a door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber for holding a culture vessel housing cells; and a manipulator arranged outside the second chamber for putting the culture vessel through the door section in and out the second chamber.
2. The culture apparatus according to claim 1, further comprising a first heater housed in the second chamber, for heating the culture vessel.
3. The culture apparatus according to claim 1, further comprising a temperature maintaining device maintaining the interior of the first chamber at a predetermined temperature.
4. The culture apparatus according to claim 1, further comprising an air supplying device supplying air at a constant temperature and at a pressure higher than atmosphere pressure, whereby air within the first chamber leaks through a small gap in the first chamber.
5. The culture apparatus according to claim 1, wherein the manipulator is provided with a drive motor in a section thereof other than a section which enters the second chamber.
6. The culture apparatus according to claim 1, wherein the moisture producing device comprises: a second heater for heating humidifying water supplied to the second chamber; a water temperature detecting device detecting a temperature of the humidifying water; and a heat amount controlling device controlling a heat amount of the second heater according to a water temperature detected by the water temperature detecting device.
7. The culture apparatus according to claim 6, wherein the moisture producing device further comprises: a water volume detecting device detecting a volume of the humidifying water; a humidifying water supplying device supplying humidifying water; and a water volume controlling device controlling supplying of water by the humidifying water supplying device based on a detection result of the water volume detecting device.
8. The culture apparatus according to claim 1, wherein the moisture producing device comprises: a humidifying water circulating device, provided with a heater for heating humidifying water, and supplying and circulating humidifying water at a predetermined temperature to the second chamber; a water temperature detecting device detecting a temperature of the humidifying water; and a water temperature controlling device controlling based on a result of the water temperature detecting device, a heat production amount of the heater arranged in the humidifying water circulating device and thereby adjusting a temperature of the humidifying water.
9. The culture apparatus according to claim 1, wherein the holding device is constituted of a shelf holding a plurality of culture vessels; and the door section has a plurality of small doors separately openable and closable with respect to the shelf so that the plurality of culture vessels can be separately taken out.
10. The culture apparatus according to claim 1, further comprising a fan arranged within the second chamber, producing an air volume sufficient to stir air within the second chamber.
11. The culture apparatus according to claim 1, wherein the humidity of the second chamber is higher than that of the first chamber.
12. A culture apparatus that cultures cells using culture vessels, the culture apparatus comprising: a first chamber; a second chamber housed in the first chamber, having a door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber for holding culture vessels housing cells; a temperature controlling device for heating the culture vessels to keep at constant temperature; and a manipulator arranged outside the second chamber for putting the culture vessels through the door section in and out the second chamber.
13. A culture apparatus that cultures cells using a culture vessel, the culture apparatus comprising: a first chamber; a second chamber housed in the first chamber, having a door section; a door drive device for opening and closing the door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber for holding a culture vessel housing cells; and a manipulator arranged outside the second chamber for putting the culture vessel through the door section in and out the second chamber, wherein the door section is a sliding door, opening and closing thereof being controlled by the door drive device so as to cover an opening arranged in the second chamber, and wherein the manipulator includes: an operating section acting on the culture vessel; a drive section driving the operating section; and a supplementary member for ensuring a distance between the operating section and the drive section.
The present application claims priority from Japanese application JP 2006-238924 filed on Sep. 4, 2006, the content of which is hereby incorporated by reference into this application.
The present invention relates to a culture apparatus, and more particularly to a culture apparatus for tissue culturing (automatic culturing) of, for example, animal cells.
2. Background Art
In medical science- and medical care-related fields and the like, culturing of various types of cells separated from biologic bodies is being actively performed. In order to efficiently performing this type of cell culturing, the culture environment must be brought as close as possible to the environment within the biologic body in which this cell has resided before separation of the cell. Also, in the cell culturing, contamination must be prevented. Particularly, a human can be the largest contamination source, so the interposition of a human is desirably prevented as much as possible.
For example, Patent Document 1 has disclosed a culture apparatus provided with a first chamber and a second chamber for culturing cells. In this culture apparatus, the first chamber is equipped with a plurality of air lock type port openings; and a manipulator operating by remote control or automatic control is arranged in the first chamber. This manipulator is accessible to both the air lock type port openings and the second chamber. Accordingly, the contamination of the culture apparatus by a human can be eliminated.
[Patent Document] JP Patent Publication (Kokai) No. 2004-236514
However, in the culture apparatus disclosed in Patent Document 1, the through control of temperature and humidity environment has not been addressed. The humidity of environment for culturing cells within a culture apparatus is ordinarily 100%. Consequently, in the culture apparatus of Patent Document 1, mechanical devices having a movable part such as a motor, including the manipulator, are exposed to the environment of humidity of 100%, and thus dew condensation, resulting corrosions, and the like adversely affects the devices, which is not preferable.
To address these circumstances, the present invention has been devised, and provides a culture apparatus capable of setting preferable environments for each element of a small-sized culture chamber and manipulator, allowing an automatic culturing operation.
To solve the above problem, a culture apparatus according to the present invention is provided with a first chamber and a second chamber being a culture chamber, and a device for producing moisture is arranged only within the second chamber. Accordingly, a difference can be produced between the humidity environment of the first chamber and that of the second chamber, and thus a manipulator arranged outside the second chamber, accessible to the second chamber can be prevented from being adversely affected by moisture.
The device for producing moisture maintains the humidity at a predetermined level using water supplied to a floor (bottom) within the culture chamber. Also, the device is provided with a heater for maintaining the temperature of this water at a predetermined level and a temperature sensor monitoring the water temperature; the heat production amount of the heater is controlled based on information of the temperature sensor. Further, when the water is supplied according to reduction thereof, it is also possible to deal with a culture processing over a relatively long period of time, and favorable culture environment can be maintained for a long period of time.
Also, a heater for heating culture vessels is arranged within the second chamber, allowing more stable maintenance of culture environment.
Further, regarding the manipulator, when a drive motor thereof is disposed at a section thereof other than a section (operating section) which enters the second chamber, the manipulator can be prevented from being affected by moisture.
More specifically, to solve the above problem, according to the present invention, there is provided a culture apparatus that cultures cells using culture vessels, the culture apparatus including: a first chamber; a device controlling a temperature within the first chamber; a second chamber housed in the first chamber, having a door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber for holding culture vessels housing cells; and a manipulator arranged outside the second chamber for putting the vessels through the door section in and out the second chamber. The humidity of the second chamber is higher than that of the first chamber.
This culture apparatus is further provided with a first heater housed in the second chamber, for heating the culture vessels.
This culture apparatus is further provided with a temperature maintaining device maintaining the interior of the first chamber at a predetermined temperature.
This culture apparatus is further provided with an air supplying device supplying air at constant temperature and at slightly higher pressure than atmosphere pressure, whereby the internal air leaks through a small gap in the first chamber.
The manipulator is provided with a drive motor in a section thereof other than a section which enters the second chamber.
One embodiment of the moisture producing device includes: a second heater for heating humidifying water supplied to the second chamber; a water temperature detecting device detecting a temperature of the humidifying water; and a heat amount controlling device controlling a heat amount of the second heater according to a water temperature detected by the water temperature detecting device, and may further include a water volume detecting device detecting a volume of the humidifying water; a humidifying water supplying device supplying humidifying water; and a water volume controlling device controlling supplying of water by the humidifying water supplying device based on a detection result of the water volume detecting device.
Another embodiment of the moisture producing device includes: a humidifying water circulating device, provided with a heater for heating humidifying water, and supplying and circulating humidifying water at a predetermined temperature to the second chamber; a water temperature detecting device detecting a temperature of the humidifying water; and a water temperature controlling device controlling based on a result of the water temperature detecting device, a heat production amount of the heater arranged in the humidifying water circulating device and thereby adjusting a temperature of the humidifying water.
The holding device is constituted of a shelf holding a plurality of culture vessels; the door section has a plurality of small doors separately openable and closable with respect to the shelf so that the plurality of culture vessels can be separately taken out.
The culture apparatus is further provided with a fan arranged within the second chamber, producing an air volume sufficient to stir air within the second chamber.
According to the present invention, there is provided a culture apparatus that cultures cells using culture vessels, the culture apparatus including: a first chamber; a second chamber housed in the first chamber, having a door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber for holding culture vessels housing cells; a temperature controlling device for heating the culture vessels to keep at constant temperature; and a manipulator arranged outside the second chamber for putting the vessel through the door section in and out the second chamber.
Also, according to the present invention, there is provided a culture apparatus that cultures cells using culture vessels, the culture apparatus including: a first chamber; a second chamber housed in the first chamber, having a door section; a door drive device for opening and closing the door section; a moisture producing device for humidifying the interior of the second chamber; a holding device housed in the second chamber, holding a culture vessel housing cells; and a manipulator arranged outside the second chamber for putting the vessels through the door section in and out the second chamber, wherein the door section is a sliding door, opening and closing thereof being controlled by the door drive device so as to cover an opening arranged in the second chamber; and the manipulator includes: an operating section acting on the culture vessel; a drive section driving the operating section; and a supplementary member for ensuring a distance between the operating section and the drive section.
Other features of the present invention will be apparent from the following description of preferred embodiments and the accompanying drawings.
According to the inventive culture apparatus, preferable environment can be set for each element of a small culture chamber and manipulator, allowing an automatic culturing operation.
FIG. 1 is a view illustrating a schematic configuration of a culture apparatus 1 according to a first embodiment;
FIG. 2 is a view illustrating a state where a door 3 of a culture chamber 2 according to the first embodiment is open;
FIG. 3 is a view illustrating a schematic configuration of a culture apparatus 1 according to a second embodiment;
FIG. 4 is a view illustrating a schematic configuration in which the culture apparatus 1 according to the second embodiment is provided with a device (automatic liquid level maintaining device) supplying humidifying water;
FIG. 5 is a view illustrating a configuration in which the culture apparatus 1 according to the second embodiment is provided with another humidity production device different from that of the first embodiment;
FIG. 6 is a view illustrating a schematic configuration of a culture apparatus 1 according to a third embodiment; and
FIG. 7 is a view illustrating a configuration in which the culture apparatus 1 according to the third embodiment is provided with another door section different from that of the first embodiment.
Specific descriptions of embodiments (automatic culture apparatus) of a culture apparatus according to the present invention will be given below with reference to the drawings. It should be noted that configurations illustrated in the drawings are merely exemplary of the present invention, and are not be construed to limit the scope of the present invention.
FIGS. 1 and 2 each illustrate a schematic configuration of an automatic culture apparatus according to a first embodiment of the present invention.
Referring to FIG. 1, within an automatic culture apparatus 1 being a first chamber, there are arranged: a culture chamber 2 being a second chamber constituting a closed space, having a door section 3; and a manipulator 5 for handling a small-sized culture vessel P and performing an operation such as replacement of culture medium. The outer wall of the automatic culture apparatus 1 is desirably composed of a heat-insulating material so that leaking of the internal heat is reduced to a minimum. Also, heat-insulating material, metal or the like can be used for the outer wall of the culture chamber 2. The use of a heat-insulating material for the outer wall has an advantage in that the internal heat of the culture chamber 2 hardly leaks and thus the internal temperature thereof can be controlled independently. The use of metal or the like for the outer wall has an advantage in that heat in the exterior of the culture chamber 2 can easily be transmitted to the interior thereof and at the same time, heat in the interior of the culture chamber 2 can also be transmitted to the exterior thereof and thus both the temperature environments of the first chamber and second chamber can easily be controlled at a culture temperature.
The first chamber is further provided with a heat radiator 6, a fan 7 and a heater 12 for maintaining the interior of the automatic culture apparatus 1 at a predetermined temperature. A temperature of the first chamber is measured by a temperature sensor 22 arranged in the outer side of the culture chamber 2; and the heat production amount of the heater 12 is controlled by an external controller (not illustrated) so that the temperature of the first chamber is maintained at a predetermined temperature: in this case, at a temperature during culturing (culture temperature: 37° C., for example).
The culture chamber 2 is provided in the interior thereof with a turn table 4 receiving the small-sized culture vessels P, a heater 10 for heating the turn table 4, and a temperature sensor 20 detecting a temperature of the turn table. The heat production amount of the heater 10 is controlled based on a temperature signal from the temperature sensor 20 by an external controller (not illustrated) so that the turn table 4 (and the small-sized culture vessel P) is maintained at a predetermined temperature: in this case, at the temperature during culturing.
The culture chamber 2 is provided in a bottom surface thereof with water W used for humidification, a heater 11 for heating the water W, and a temperature sensor 21 detecting a temperature of the water. The heat production amount of the heater 11 is controlled based on a temperature signal from the temperature sensor 21 by an external controller (not illustrated) so that the water W is maintained at a predetermined temperature: in this case, at the temperature during culturing.
The manipulator 5 is provided with a drive section (actuator A) for ensuring flexibility required for operation, and an end effector E.
In a state where culturing is under way with the small-sized culture vessel P placed within the culture chamber 2, the interior of the culture chamber 2 is maintained at the culture temperature and the water on the floor surface of the culture chamber 2 is being vaporized. Further, since the external atmosphere of the culture chamber 2 is maintained at a culture temperature by the heater 12 arranged within the culture apparatus 1, the interior of the culture chamber 2 is maintained at the culture temperature and at a humidity of 100%. Further, since the first chamber is isolated from the culture chamber 2 (second chamber) by the door section 3, the humidity of the culture chamber 2 is higher than that of the first chamber.
As illustrated in FIG. 1, the manipulator 5 is disposed outside the culture chamber 2, and the door 3 of the culture chamber 2 is being closed. Thus, the humidity of the interior of the culture chamber 2 does not affect the exterior thereof. Accordingly, the problems such as dew condensation on the manipulator 5 and resulting corrosions can be solved.
The culture apparatus 1 is also provided with an air supplying section 13 that supplies clean and constant-temperature air from the outside at a pressure slightly higher than atmosphere pressure; thus, the internal air leaks through a minor gap (not illustrated) of the culture apparatus 1. Consequently, a small amount of air leaks at all times and thus the air within the culture apparatus 1 is replaced bit by bit.
When an operation such as replacement of culture media takes place, as illustrated in FIG. 2, the door 3 of the culture chamber 2 is opened. This opening and closing operation of the door 3 is performed by a drive section (not illustrated) arranged outside the culture chamber 2 based on an instruction from an external controller (not illustrated). As illustrated in FIG. 2, with the door 3 opened, the manipulator 5 moves; and the end effector E holds the small-sized culture vessel P and a predetermined operation is performed in the interior or exterior of the culture chamber 2. When replacement of culture media is performed, culture media is supplied via a tube (not illustrated) extending to the end effector E, and the waste liquid is collected.
While the door 3 is open, the internal moisture leaks to the first chamber. However, since it does not take much time to perform the replacement of culture media, the humidity within the culture apparatus 1 will never change to the same level (100%) as the interior of the culture chamber 2. Further, the leaked moisture is gradually lessened by supplying clean air into the culture apparatus 1 to recover the original humidity. Accordingly, adverse effects of the leaked moisture can be neglected.
After the operation, the small-sized culture vessel P is returned to its original position, and the manipulator 5 retreats, and the door 3 is closed, and the culture environment is recovered.
According to the automatic culture apparatus 1 having the above described configuration and function, the 100% humidity environment preferable for the small-sized culture vessel P can be provided and at the same time the low humidity environment preferable for the manipulator 5 used for operation can also be maintained.
A second embodiment of the present invention will be described below with reference to FIGS. 3 to 5. FIG. 3 illustrates a schematic configuration of an automatic culture apparatus 1 according to the second embodiment of the present invention. In the automatic culture apparatus of FIG. 3, the configuration of a temperature maintaining device within the culture apparatus 1 is different from that of the first embodiment.
Referring to FIG. 3, gaps 9a, 9b and 9care arranged in walls of each chamber within the culture apparatus 1. In FIG. 3, a fan 7, filter 8 and heater 12 are arranged in the gap 9a; the fan 7 sends air to the heater 12. The heat production amount of the heater 12 is as with FIG. 1, controlled from the outside based on temperature information of the temperature sensor 22 arranged outside the culture chamber 2 so that a constant temperature is obtained. When this state is maintained, the air is circulated via the gaps 9b and 9cto reach gradually to a constant temperature; and at the same time, the internal microscopic particles are captured by the filter to allow raising of the cleanness within the culture apparatus 1.
The automatic culture apparatus according to the second embodiment is as illustrated in FIG. 4, provided with a device automatically maintaining at a constant level the amount of water residing on the floor surface of the culture chamber 2 for humidity maintenance. More specifically, a water level detection sensor 30 monitors a level of water, and detects an amount of water which decreases when evaporation occurs and when humidity leaking at the time of opening the door 3 causes water to evaporate; and an external controller (not illustrated) determines a water supplying amount; and a pump 32 is driven to supply water W via a supplying tube 31 from an external water reserver tank 33. Typically, tissue culturing takes several weeks of culture period, so the capability of automatically supplying water over a long period is a function required for the automatic culture apparatus.
The automatic culture apparatus according to the second embodiment is as illustrated in FIG. 5, also provided with a humidity production device having a configuration different from that of the first embodiment. Referring to FIG. 5, heated water on the floor surface of the culture chamber 2 enters a constant temperature water unit 45 called a circulator via discharging water side tubes 41 and 43, and passes through a temperature adjustment system therein and a pump, and returns to the interior of the culture chamber 2 via supply side tubes 44 and 42. Accordingly, the water is circulated, and heated by a heater (not illustrated) arranged within the circulator 45 to maintain the water temperature at a constant level, and at the same time the humidity within the culture chamber 2 is maintained at 100% by causing the water to evaporate. Also, the water temperature within the culture chamber 2 is monitored by a temperature sensor 21 arranged within the culture chamber 2, and when the water temperature is lower than a predetermined level, the heat production amount of the heater of the circulator 45 is adjusted by an external controller (not illustrated), whereby the temperature can be efficiently controlled. In this case, the temperature sensor may be arranged within the circulator 45 to control the water temperature.
An automatic culture apparatus according to a third embodiment of the present invention will be described with reference to FIGS. 6 to 7. FIG. 6 illustrates a configuration of an automatic culture apparatus 1 different from that of the first embodiment in the method of receiving the small-sized culture vessel P. Referring to FIG. 6, the culture chamber 2 is provided with multiple shelves receiving a plurality of small-sized culture vessels P, and respective shelves are provided with doors 3a, 3b and 3c independently openable and closable. Instead of being separately opened, these doors may be opened all at once, or may be constituted of a single door. However, when the doors are separately opened and closed, the opening area of the culture chamber 2 can be reduced and thus adverse effects of humidity on the manipulator 5 can be comparatively reduced.
Further, in order to stir the internal air to reduce unevenness of the internal humidity and at the same time to maintain the temperature within the culture chamber 2 at a predetermined culture temperature, the culture chamber 2 is provided with a fan 52 producing an appropriate air volume, a heater 51 for maintaining the temperature at a predetermined culture temperature, and a temperature sensor 50 used for control. In this case, though not illustrated herein, in order to prevent humidity irregularity, there may be arranged a fin for dispersing and guiding the air sent by the fan 52, and a fixed rectifier board.
When a processing such as replacement of culture media is performed in the small-sized culture vessel P, for example, the door 3a is opened, and the end effector E of the manipulator 5 enters the interior of the culture chamber 2 and grasps the small-sized culture vessel P and then the processing is performed in the interior of the culture chamber 2 or after transferring the small-sized culture vessel P to the outside. After completion of the processing, the small-sized culture vessel P is placed in its original position, and the manipulator 5 retreats from the culture chamber 2. With another small-sized culture vessel P in another position, also, the corresponding door is similarly opened to perform the processing. Accordingly, the interior of the culture chamber 2 of a humidity of 100% is completely separated from the interior of the culture apparatus 1 where the manipulator 5 is placed, thus maintaining the environments adapting to the respective functions.
FIG. 7 illustrates an automatic culture apparatus 1 having a door of a structure different from that of the first embodiment. Referring to FIG. 7, the partition wall of the culture chamber 2 is provided with a gap (opening) 60, and there is a door 63 (for example, a sliding door) arranged so as to cover the gap. A movable section 62 of this door 63 is moved vertically by a vertically driving mechanism 61 to vertically move the door 63 secured in an integrated manner to the movable section 62, whereby a door function is provided. Corresponding to this door mechanism, the manipulator 5 is provided with a supporting member (supplementary member) F between an end effector E and an actuator A driving the effector E; when the door 6 is opened and the end effector E is made to enter the interior of the culture chamber 2, the actuator A which is the drive section can be placed at a longest distance from the interior of the culture chamber 2. In this way, there is used a structure in which the supporting member F allows the end effector E to be placed at a certain or more distance from the actuator A. Consequently, the actuator A of the manipulator that is particularly vulnerable to adverse effects of humidity and the like, can be placed at a longest distance from the high humidity environment, and thus the adverse effects of humidity can be prevented more efficiently. As evident from comparison with the structure of FIG. 3, the distance between the actuator and end effector can be lengthened in the structure of FIG. 7.
Though not illustrated, when a moisture absorbent material absorbing moisture leaking during opening of the door is arranged at a predetermined position within the culture apparatus 1, an environment more suitable for the manipulator 5 (particularly, actuator A) can also be maintained.
Also, when the actuator A is covered with a protective member such as resin, the adverse effects of humidity can be further prevented.
Further, in the first to third embodiments, water is supplied to the floor surface (bottom surface) of the culture chamber 2 and heated to obtain a humidity of 100%, but the present invention is not limited thereto; air of a humidity of 100% may be supplied to the culture chamber 2. In this case, there can be used a configuration in which the culture chamber 2 is provided with a device monitoring an internal humidity, and air of a culture temperature, containing moisture is sent to the interior of the culture chamber 2 based on a result thereof.
According to the above described culture apparatus of the present embodiments, the first chamber and the culture chamber (second chamber) are separated; the moisture producing device is arranged only in the second chamber; and both the chambers are maintained at a culture temperature. Accordingly, the small-sized culture vessel can be placed (maintained) in the environment of the culture temperature and a humidity of 100%, and the manipulator used for culturing operation can be placed in the environment of the culture temperature and a low humidity. Consequently, the entire manipulator can be prevented from being exposed to a high humidity area, thus allowing prevention of corrosions and the like of the manipulator. Also, since the drive section of the manipulator affected by humidity in the manipulator is arranged at a position other than the section thereof functioning in the high humidity area, e.g., the end effector, adverse effects of humidity can be further reduced.
Further, since the heater is also arranged in the turn table, the temperature control of the turn table (and the culture vessels) can be quickly performed, so the temperature can be maintained at a constant level even during opening/closing of the door. Also, water for supplying moisture can be automatically supplied and thus the environment can be automatically maintained over a culturing period.
Also, since the temperature maintaining device for maintaining the interior of the first chamber at a predetermined temperature is arranged, the first chamber and second chamber can be controlled at the same temperature.
Further, the culture apparatus according to the present embodiment is provided with the air supplying device supplying air at a constant temperature and at slightly higher pressure than atmosphere pressure and thus the internal air leaks through a small gap of the first chamber. Accordingly, even when the door section is open and the moisture of the second chamber leaks to the first chamber, air having no moisture is supplied to the first chamber, thus allowing suppression of adverse effects of moisture on the manipulator.
When the manipulator is provided with the drive motor in a section thereof other than a section entering the second chamber, a distance between the motor and culture vessel can be ensured, allowing suppression of adverse effects of moisture on the motor.
Concerning the regenerative medicine which cultures cells to utilize them for medical treatment or to examine them, there have been reported examples which exhibit innovative accomplishments at the laboratory level. However, in order for the regenerative medicine to popularize as a typical medical treatment, it is an important point how safely high-quality cultured tissues are efficiently produced. For this, not only culturing by human hands but also efficient automatic culturing is needed. In order to set it up in industry, the establishment of the automatic culturing technology is urgently needed. In the automatic culturing technology, a culturing processing by machine is indispensable; in this case, the environment required for culturing is not always identical to the placement environment required for the machine. Thus, the applicability of the present invention which can perform processings while maintaining environments suitable for each element is large.
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Man Utd boss Erik ten Hag working on summer transfer - days after World Cup humiliation
It's believed Erik ten Hag wants to revamp Manchester United's goalkeeper department with the futures of David de Gea, Dean Henderson and Martin Dubravka all in doubt
Tom BlowSports Writer
Erik ten Hag is looking at ways to improve his Manchester United squad (Image: Ash Donelon/Manchester United via Getty Images)
Manchester United manager Erik ten Hag is reportedly interested in signing Yann Sommer on a free transfer next summer.
Sommer's Borussia Monchengladbach contract expires at the end of the season and the 33-year-old could find employment elsewhere. The Swiss goalkeeper played in his country's humiliating 6-1 defeat to Portugal in the last 16 of the World Cup on Tuesday.
United will be able to contact Sommer's agent from January, as players can speak to overseas clubs during the final six months of their contracts. The Red Devils are looking to revamp their goalkeeping department due to the uncertainty surrounding current stars.
As reported by ESPN, United are considering signing Sommer on a pre-contract agreement ahead of next season. The 80-cap international has represented Switzerland at five major tournaments and played more than 600 first-team games at club level.
Sommer started his career at Swiss giants Basel. After loan spells at Swiss Super League rivals Vaduz and Grasshoppers, he became Basel's first-choice goalkeeper and won four consecutive league titles, as well as the Swiss Cup during the 2011/12 campaign.
Sommer joined Monchengladbach in the summer of 2014 as their first-choice goalkeeper. Although he is yet to win silverware in Germany, he has played in the Champions League was named in the Bundesliga Team of the Season during the 2019/20 term.
Yann Sommer is believed to be a transfer target for Manchester United (Image: Getty Images)
Do you think Manchester United should sign Yann Sommer? Let us know in the comments below!
"I am currently having very open talks with the club [about a new contract]," said Sommer in the summer. "I admit that it took me a lot of time to process the past season and to think about everything that happened [Monchengladbach finished 10th in the Bundesliga]."
United boss Ten Hag knows he may need to bring in new goalkeepers in the next few transfer windows. Martin Dubravka's loan spell expires at the end of the season and he is expected to return to Newcastle after playing just once for the Red Devils this season.
Fellow reserve stopper Tom Heaton is out of contract in June - although United have the option to extend his deal by a further 12 months - while Dean Henderson is not expected to re-join Ten Hag's squad once his loan spell at Nottingham Forest ends in June.
Henderson was critical of United after joining Forest on a one-year loan deal in the summer and it's hard to imagine a route back into Ten Hag's squad. There are also doubts about the future of United's undisputed first-choice goalkeeper David de Gea.
The Spaniard's contract expires in June, although United can extend his deal by a further 12 months. De Gea is one of their highest-paid players - earning around £375,000-a-week - and they could be reluctant to give him another long-term contract on the same wage.
Rumours of United's interest in Sommer also begs the question of whether he would be Ten Hag's first-choice goalkeeper or De Gea's understudy. Sommer may be reluctant to be a second-choice stopper, seeing as he's been a No.1 for the previous 11 seasons.
Transfer deadline day
What time and TV channel is Roscommon v Tyrone on today in the Allianz football league?
Jake Paul vs Tommy FuryJake Paul makes Tyson Fury laugh with comments about disowning brother TommyPaul repeated Tyson's vow about disowning brother Tommy if he loses in their grudge fight and the heavyweight champion couldn't hold in his laughter
Cristiano Ronaldo invites four ex-Man Utd teammates to visit him including Harry Maguire
Harry Maguire is amongst four Manchester United players to be invited by Cristiano Ronaldo out to Saudi Arabia following the 37-year-old's lucrative switch to Al-Nassr
Ireland striker Michael Obafemi completes loan move to Burnley
Republic of Ireland Soccer Team
The Republic of Ireland striker has joined until the end of the season, with the option of making the deal permanent in the summer
National Football LeagueRoscommon v Tyrone and Donegal v Kerry LIVE score updates, TV and stream details, start time and more for the Allianz League tiesFollow all the action from the Allianz Football League Division 1 clashes
Olivia Wilde and Jason Sudeikis spotted hugging as they reunite after custody battle
Olivia Wilde and her ex partner Jason Sudeikis have been battling it out over their two children after they split in 2020. The former couple were engaged for seven years before going their separate ways

redpajama_set_name: RedPajamaCommonCrawl


The extreme temperatures and the absence of moisture in the air have badly hit the mango farms in Ramanagaram district, and the output is expected to decline drastically this season.
According to the Horticulture Department, the situation is similar in other areas growing mango across the State.
Sources say the total production is expected to dip by 20 to 25 per cent this time.
Mango is cultivated on around 1.7 lakh hectares in the State.
While the fruit is grown on 50,000 hectares in Kolar district, it is cultivated on 25,000 hectares in Ramanagaram district. Raspuri, Alphonso, Malgova, Bainganpalli, Neelam, Mallika, and Thothapuri are some of the popular varieties grown in Karnataka.
The production this season is likely to hover between 7.5 lakh and eight lakh tonnes. The target was nine lakh tonnes, a senior official in the department said.
Of this, Kolar growers are expected to produce 4.2 lakh tonnes, while the farmers in Ramanagaram are expected to produce 2.5 lakh tonnes.
The prevailing drought conditions have severely affected the mango farms in Ramanagaram.
Hundreds of mango trees in Ramanagaram and Magadi taluks have already withered because of the non-arrival of rain, says Thumbenahalli Shivakumar, a mango grower and senior Karnataka Rajya Raitha Sangha (KRRS) leader.
Mr. Shivakumar is one of the many growers who have suffered huge losses due to the fruit-droppings caused by the lack of moisture in the atmosphere.
In the past three weeks, more than 2,000 fruits have dropped at his farm in Thumbenahalli. "The size of fruits has also shrunk significantly," he adds. According to a Nagaraj, a wholesale trader, the prolonged drought conditions have significantly brought down productivity across the State.
He says the mango season has commenced and the fruit has started arriving in domestic markets in almost all districts, but the size of all varieties has shrunk by around 25 per cent.
According to K.N. Roopashree, Deputy Director, Department of Horticulture (Ramanagaram), the production fall estimation is 20 per cent.
"Ramanagaram is one of the major mango producing districts in South India. Fruits dropping off trees is being observed in almost all the taluks," she says.
Also, fungal infection has affected crops in some parts of the district, Ms. Roopashree adds.
And with supply set to take a hit, the price of mangoes is expected to increase in the domestic markets.


text:
Home » Editions » 2016 04 July/August » AlterRurality
AlterRurality
DOMINIC STEVENS (DUBLIN SCHOOL OF ARCHITECTURE, DIT) AND SOPHIA MEERE (LANDSCAPE ARCHITECTURE, UCD) DISCUSS THE 'FIELDWORK LETTERFRACK 2016: ALTER-RURALITY' CONFERENCE, WHICH THEY RAN 6 – 9 JUNE IN RURAL CONNEMARA.
What starts to happen when artists, architects, landscape architects, farmers, practitioners and researchers from all around Europe meet to discuss rural life? This June, in Letterfrack, Connemara, an event was held to find out. Three Irish universities (University College Dublin, Dublin Institute of Technology and Galway Mayo Institute of Technology) gathered together 65 researchers, practitioners, teachers and advisors, all engaged with rural life and interested in its future, from 30 different organisations and 10 different countries, for exchange of ideas and experience.
"We do not belong to those who have ideas only among books. It is our habit to think outdoors – walking, leaping, climbing, dancing, preferably on lonely mountains or near the sea where even the trails become thoughtful." (Friedrich Nietzsche)
The primary purpose of this conference was to explore possible futures, opportunities and challenges for rural milieus in twenty-first-century Europe. This meeting of minds had several aims, however, one of them being to cause some kind of convergence between various strands of the design community and the rural community itself. We thought it would be a good start for a group whose ultimate purpose is to imagine viable, productive and lively rural opportunities.
By gathering together attendees with a wide range of experience, and spending each day debating a particular question or theme, we hoped to encourage new dialogues across disciplines. By making the space and taking the time to immerse ourselves in place – walking, talking, thinking, looking, tasting and seeing – we hoped to create an exchange of knowledge and ideas about rural landscapes, lifestyles, production, design and culture, looking at how best to sustain rural places.
Intense exchanges took place in the formal setting of a lecture theatre (3 mornings: 24 plenary presentations, 3 evenings: 9 keynotes), but unlike many academic conferences, the possibilities for informal discussion and exchange also abounded. Lunch, teas and dinner were served in the village's Ellis Hall, thanks to local chef Derick Healy and his team. Participants spent their afternoons leisurely foraging on the shore, tracing watercourses, visiting farms or simply walking and talking. All were helped by the unexpected sunshine!
Holding this conference in a rural setting was important. Organised as a collaboration between ourselves and Deirdre O'Mahony from GMIT's Centre for Creative Arts and Media (CCAM), this was the third in a series of events held under the name 'AlterRurality'. The first was held in Fribourg, Switzerland and the second in London. These events were ARENA projects, an international network that exists to promote research in architecture and allied disciplines across Europe.
GMIT's Letterfrack National Centre for Excellence in Furniture Design proved the perfect setting for a conference on rurality, as the village has an extraordinary history of rural development, in which the school buildings play a significant role.
Thirty-three talks
The conference kicked off with Pieter Versteegh's (Ecole Spéciale d'Architecture, ESA Paris) keynote lecture offering an alternative description of rurality in which a provocative list of presumptions are inverted, or as Pieter put it, "viewed through a mirror".
In addition to invited keynote speakers, 24 papers were chosen for oral presentation, with 24 more made into posters and displayed in Ellis Hall, which is owned by Connemara West. Short presentations succeeded one another in response to specific themes. The sessions and the succession of papers were chosen as counterparts to each other, in order to expand the field of view as far as possible. While some talked of real places and things, others talked of the metaphysical and of transformation. We all talked about time: past, present and future.
Outstanding presentations included that of Dr Ciara Healy (Lecturer in Art, Department of Art, University of Reading) and Adam Stead (visual artist) in a collaborative performance in which correspondence between teacher and student were read aloud. Titled Already the world: A Post-humanist Dialogue, their letters were concerned with ecological and environmental ways of knowing in socio-agricultural and metaphysical modes. With Healy's help, Stead sought to understand the socio-political and ecological impacts of industrialisation and consumerism on agriculture and rural communities in Britain and Ireland.
In Bows, Buildings and Experimental Archaeology: A Journey from Norway to Dublin Through Bowmaking, Stephen Fox, an experimental archaeologist and PhD student at UCD, described how both questions and answers can arise through making. Fox is an expert in Viking archery and bow making. Having spent the summer of 2015 working in a reconstructed longhouse museum in Lofotr, Norway, he is currently investigating Dublin's Viking architecture, its raw materials and construction methods through the active building of an authentic type 1 Viking house at UCD's Centre for Experimental Archaeology and Material Culture.
In the following presentation, Digitally Fabricating Rural Wood Constructions, Professor Urs Hirschberg of TU Graz's Institute of Architectural Media transcended 1000 years to promote sophisticated digital fabrication technologies, the building of bespoke timber structures in rural areas and, conversely, the showcasing of special timber designs in urban contexts. He demonstrated both the viability of high-tech firms operating from rural locations and the possible revival of craft in the digital age. TU Graz's IAM works with local industry partners to push the boundaries of timber fabrication, teaching future architects and fabrication factories about the amazing possibilities of the digital tools.
Andrew Freear's description of Auburn University's famous design and build programme left us wondering why we can't all do the same thing! Known for its ethos of recycling, reusing and remaking, Rural Studio provides hands-on educational experience while assisting an underserved population in west Alabama's Black Belt region. The studio's philosophy is that everyone, rich or poor, deserves the benefit of good design. Students work within the community to define solutions, fundraise, design and, ultimately, build remarkable buildings. In 25, Rural Studio has built 150 projects and educated more than 600 'citizen architects'.
Dr Aine Macken-Walsh, researcher with Teagasc, astounded the audience by her proposition that governmental policies of the past 25 years have largely failed to support Irish farming and fishing families. Funding, she argued, has created a 'project class' that has benefitted a few, but been ignored by the majority.
Chinese researcher and PhD scholar at London's Architectural Association, Jingru Cyan Cheng, suffers no illusions: rurality in China is administratively determined, socially constructed and spatially specified.
Her design research project instrumentalises rural China's specific administrative, social and spatial conditions to explore rurality as a spatial question. It proposes a spatial framework for China's rural territory in an attempt to inverse the power of the city.
Three walks
The point of the walks was to be outside, to breathe fresh air and to walk and talk. Aided by unusually low tides and bright sunshine, Rory Keatinge led wonderful walks along the shore, taking us to his secret locations to collect cockles and mussels, before helping us clean and prepare them for dinner. Rory has foraged for food along the coast of Letterfrack since his childhood, a passion that undoubtedly contributed to his choice of profession in fisheries management.
In her booklet The Water Glossary, artist Carol Anne Connolly gathered together Irish terms that describe water, essentially reproducing a descriptive landscape in words sourced from old texts as well as from a diverse range of people including fishermen, farmers, weather forecasters, scholars and poets. Connolly read aloud from her lexicon in the Irish language while we admired the view, the sounds of water and her words.
Michael Gibbons, one of Ireland's leading field archaeologists and a storyteller, took us on a walk to the far and not so distant past of the hills, pointing out rocks and patterns in the landscape, traces of ancient settlements and tombs that indicate animal enclosures and field systems. He also identified more recent burial grounds and abandoned cottages dating from the Famine.
Located in an extraordinarily beautiful setting overlooking Killary Fjord, we walked across hills on which traditional blackhead horny sheep run as Tom Nee of Killary Sheep Farm described the highs and lows of contemporary farming. His talented dogs demonstrated sheepherding moves while Nee cut turf by hand using the old two-sided spade, or sleán, which cuts through the peat as if it were butter. Tom also talked about the relationship between farming and tourism.
Three Dinners
Immersed in rural matters we indulged ourselves in local food and the eating of communal meals cooked and served daily. Three dinners. Three lunches. Three cream teas of scones and homemade strawberry jam. Connemara lamb, mashed potato and cabbage. Roast chicken. Fish stew. Mussels, cockles gleaned by the beach walkers. Rhubarb crumble. Home-grown watercress. Nettle soup.
'Fieldwork Letterfrack 2106: AlterRurality3' built on the experience of two earlier 'ARENA' conferences. Held in a rural locality, the event remained intentionally small. It brought together a range of disciplines, teachers, practitioners and researchers from Ireland and beyond, with the aim of encouraging informal debate and exchange across disciplines. It widened participation to include the community voice with the aim of (starting to) overcome the barriers that still separate academia from practice. We believe that this conference is a step in the right direction, and a move towards greater collaboration between the many institutions, practitioners and academics that are engaged in rural life and interested in its future.
Sophia Meeres teaches at UCD. Her research is connected to the transformation of landscapes, the recognition and conservation of rural lands, practices and traditions.
Dominic Stevens is an architect and lecturer in the Dublin School of Architecture DIT whose works and practice is often rurally located.
Images: Attendees at the 'AlterRurality' conference; Attendees on one of the walks in Connemara
Architecture, Community, Connemara, Dublin Institute, DUBLIN SCHOOL, Letterfrack National Centre, Rural, RURAL CONNEMARA, University College Dublin


It feels like a lot of work because it is. It feels exhausting because it is. Don't minimize what you're doing for yourself.
It's almost like raising yourself from scratch. It's like starting over. It's major construction, excavation and strengthening. It is a ton of work.
It's taking your young self's hand and guiding them through the hard times. It's looking back and integrating your past and your present. It's forgiveness. Its kindness and compassion. Accepting that many things can only be learned by making mistakes and by doing things wrong. It's taking hindsight and moving forward instead of looking back. It's opening old wounds once and for all so that the healing process can begin. It's filling gaps with better things. It's learning and unlearning. It's integrating your past, present, and future selves, of which there are infinitely many, and it's necessarily a lifelong process. But a worthwhile one at that.
I sometimes find it hard to take on this job of healing, just because it is hard. You are touching on my subconscious!


text:
Hassani Shapi (born 15 July 1973), is a Kenyan actor, particularly active in Italian cinema. He is best known for the roles in the films Star Wars: Episode I - The Phantom Menace, The World Is Not Enough and Il maresciallo Rocca.

Personal life
He was born on 15 July 1973 in Mombasa, Kenya. He obtained his masters degree in Paris, France, in veterinary science. After becoming a veterinary doctor, he worked in a private clinic in Paris. After few years he quit the job to get into acting.

Career
He first worked with an English theatre company in Paris called ACT. He has been involved in theater and played in several plays such as One Flew Over the Cuckoo's Nest, Animal Farm, The Old Man and the Sea and Don Quixote. In 1992, he made his television debut in some episodes of the series Runaway Bay directed by Tim Dowd. After six years in 1998, he reappeared in television for a single episode of the serial The Ambassador. In 2009, Shapi starred in the role of 'Saddam Hussein' in the Italian television serial Intelligence - Servizi & Secrets.

After he moved to America, he joined the cast of Law & Order in 2010. However, his most notable film appearance came through the role of Jedi Master 'Eeth Koth' in Star Wars: Episode I - The Phantom Menace  which was filmed by George Lucas in 1999. In the film, he acted along with renowned actors, Liam Neeson, Ewan McGregor and Natalie Portman.

With the success of Star Wars, he was invited to taken part in several major international productions such as Agent 007, The world is not enough, Irina Palm and Don't desire the woman of others. Meanwhile, he appeared in several Italian comedies such as Our Marriage Is In Crisis, Lezioni of Chocolate, Today Married, Sharm el Sheikh - An Unforgettable Summer, Nobody Can Judge Me, Females Versus Males, Without Art or Part, Chocolate Lessons 2 and The Extra Day With Fabio Volo.

Filmography
 Star Wars: Episode I - The Phantom Menace (1999)
 The World Is Not Enough (1999)
 Our Marriage Is In Crisis (2002)
 Don't Desire the Other Woman (2004)
 Murder Unveiled (2005)
 Irina Palm (2007)
 Lessons in Chocolate (2007)
 Just Married (2009)
 Oggi sposi (2009)
 Sharm el Sheikh - An Unforgettable Summer (2010)
 Women vs. Men (2011)
 No One Can Judge Me (2011)
 Without Art or Part (2011)
 Chocolate Lessons 2 (2011)
 One Day More (2011)
 Escort in Love (2011)
 Land Gold Women (2011)
 The Extra Day (2011)
 Make a Fake (2011)
 Damascus Cover (2017)
 Scappo a casa (2019)
 Pop Black Posta (2019)

Television
 Runaway Bay (1 episode, 1992)
 The Ambassador (1 episode, 1998)
 Murder Prevention (1 episode, 2004)
 Apparitions (2008)
 Intelligence - Services & Secrets (3 episodes, 2009)
 Inspector Coliandro (3rd season, episode 4, 2009)
 Law & Order: UK (1 episode, 2010)
 The Island (2012)
 Welcome to Table 2 - North vs South (1 episode, 2013)
 Anti-Mafia Squad - The Boss Returns Episode 8 (2016)
 The Island of Pietro (2017)

References

External links

Living people
Kenyan male film actors
Italian male film actors
1973 births


In this paper we present preliminary results related to path-planning problems when it is known that the quantities of interest in the system are generated via a diffusion process. The use of mobile sensor-actuator networks (MAS-Net) is proposed for such problems. A discussion of such networks is given, followed by a description of the general framework of the problem. Our strategy assumes that a network of mobile sensors can be commanded to collect samples of the distribution of interest. These samples are then used as constraints for a predictive model of the process. The predicted distribution from the model is then used to determine new sampling locations. A 2-D testbed for studying these ideas is described. The testbed includes a network of ten robots operating as a network using Intel Motes. We also present simulation results from our initial partial differential equation model of the diffusion process in the testbed.


text:
Houston Texans Have A Great Deal Of Cap Space
What will they do with it?
By Tim Apr 13, 2017, 1:51pm CDT
Share All sharing options for: Houston Texans Have A Great Deal Of Cap Space
Nuk can't believe they cut Tony Bergstrom.
Photo by Maddie Meyer/Getty Images
There was a time when your Houston Texans weren't exactly awash in cap space in comparison to other teams. That's no longer the case, thanks in large part to the Texans' bold strategy of not signing a free agent who wasn't on their team last year. With Tony Bergstrom's release today, the Texans now boast more than $31 million in cap room.
This will bump the Texans' cap space up to $31.608m with 64 player contracts.
— TexansCap (@TexansCap) April 13, 2017
Compared to the rest of the NFL, Bob McNair's organization is swimming in cap dollars.
Texans have 5th most cap space in NFL at the moment, 2 of the top 4 in same division. Indy nearly same amount of cap space.
The Colts' cap space, however, isn't what it was at the time that tweet was initially sent.
Colts sign Johnathan Hankins, per report https://t.co/mBRpAZ15dG
— Stampede Blue (@StampedeBlue) April 13, 2017
Back to the Texans—at this stage of the free agency game, it's rather unlikely that the team will make any kind of splash. What's considerably more likely is that the organization uses a good chunk of that space to extend existing players. I reckon DeAndre Hopkins is that the top of potential candidates for that treatment.
You know, Nuk...the dude who has been making catches like this since he was knee-high to a grasshopper.
Throw back to the highschool days. pic.twitter.com/FGp6B07jxa
— Deandre Hopkins (@Nukdabomb) April 13, 2017


President Obama: "Well, first of all I think Cory Booker is an outstanding mayor. He's doing great work in Newark and obviously helping to turn that city around. And I think it's important to recognize that this issue is not a, quote, distraction. This is part of the debate that we're going to be having in this election campaign about how do we create an economy where everybody from top to bottom, folks on Wall Street and folks on Main Street, have a shot at success, and if they're working hard and they're acting responsibly, that they're able to live out the American dream.
Now, I think my view of private equity is that it is — it is set up to maximize profits and that's a healthy part of the free market. That's – that's part of the role of a lot of business people. That's not unique to private equity, and as I think my representatives have said repeatedly, and I will say today, I think there are folks who do good work in that area, and there are times where they identify the capacity for the economy to create new jobs or new industries. But understand that their priority is to maximize profits. And that's not always going to be good for communities or businesses or workers.
And the reason this is relevant to the campaign is because my opponent, Governor Romney, his main calling card for why he thinks he should be president is his business experience. He's not going out there touting his experience in Massachusetts. He's saying,' I'm a business guy and I know how to fix it,' and this is his business.
And when you're president, as opposed to the head of a private equity firm, then your job is not simply to maximize profits. Your job is to figure out how everybody in the country has a fair shot. Your job is to think about those workers who get laid off and how are we paying them for their retraining? Your job is to think about how those communities can start creating new clusters so that they can attract new businesses. Your job as president is to think about how do we set up a equitable tax system so that everybody's paying their fair share that allows us then to invest in science and technology and infrastructure, all of which are going to help us grow.
And so, if your main argument for how to grow the economy is, 'I knew how to make a lot of money for investors,' then you're missing what this job is about. It doesn't – it doesn't mean you weren't good at private equity, but that's not what my job is as president.


It sounds completely counterintuitive that a razor-sharp knife would actually cause fewer injuries in the kitchen, doesn't it? An interview with a butcher in a recent Gourmet Live article reveals how this is so.
Stanley Lobel of Lobel's butcher shop in New York explains that a sharp knife means you have to make fewer cuts. A dull knife makes you work harder; several cuts are required where one or two would do.
It's really a simple law of averages: fewer cuts means fewer chances of cutting yourself over the long run. More cuts, and the risk goes up. A sharp knife will also cut more cleanly and precisely than a dull knife, and with much less chance of slippage.
A few other knife safety tips from the article: make sure you cut away from your body at all times, keep the handle of your knife (and your palm) dry, and don't leave knives in the sink where they can become hidden under soapy water.


Do you know, with few clicks you will be able to create Virtual Machine in Windows Azure. In this article I am going to create Virtual Machine in Windows Azure. Let's see how easy to create virtual machine in windows Azure.
Now you may click on the Virtual Machine to create new Virtual machine and also to see all the existing virtual machine.
Here you can see the status of all the existing Virtual Machine. You always have to make sure that your virtual machine is topped/shutdown once you finish your work. Otherwise it will consume your data and you will be getting the bill based on the usage.
Now, to create new virtual Machine you may click on the NEW button at the left bottom corner.
You will find here an option to enter the server name, user id , password. Most important option you may find here is the IMAGE Dropdown and the SIZE of the virtual machine.
I am selecting the default size here but I require a virtual machine with Visual Studio image. So from the dropdown list I am selecting the More Images… option to find the correct image and version required. I am going to choose Windows Server 2012 with Visual Studio ultimate 2013 Update 1. Since we are selecting the image with Visual Studio you don't need to enter DNS name, User ID and password here.
Click Next to enter the DNS name, User ID and password.
Once you Click next you will find another important configuration here, that is nothing but the CLOUD SERVICE. If you click on the Cloud Service Drop down List you will find all the virtual machines you have already created. This is the place used to configure load balancing. In case you require load balancing then you have to choose one of the existing virtual machine you already created. Here I am not going to create load balancing so I have chosen Create new Cloud Service option.
Just to show you how does it works I have selected an existing Virtual machine name in Cloud Service. As soon as you select that VM you can find that DNS field name and the region fields will be disabled. The reason for this is if you want load balancing then the DNS name must be same and also the region this VM is going to create must be same.
In next wizard we will select the end point we are going to use. By default Remote Desktop and PowerShell will be added. If you wanted to host any application in this Virtual Machine we have to add HTTP endpoint as well. Otherwise we may not be able to browse the application over the internet. Click on the Tick mark to create the Virtual Machine.
Once you click on the tick mark you have to wait for some time to create the virtual machine. You can see the progress on your screen.
Once you find the status as Running you can open the Virtual machine configuration by selecting the Virtual machine name. Now click on the DASHBOARD tab to find the CONNECT option to do the remote login to the server.
You may get an alert when you click on connect button and also the download option to download the rdp file.
Once you download the .rdp file you can either double click to connect on the downloaded file or directly click on the Open button showing in the message itself.
Enter the user id and password to login to the Virtual Machine. That's it..You have created virtual machine now…You can see the desktop icon for Visual Studio 2013 in your virtual machine.
Make sure you shut down the Virtual machine if you don't require the machine. You will find the option Shutdown in virtual machine if you press "Windows + C", also once it disconnects make sure to shutdown the service in case if it is running.
To find the Shutdown option for the service you may click on the Virtual Machine menu at the left menu bar.
Hope you are good to create new virtual machine in Windows Azure now…!!!


BioLink Buffer & Penetrant is a natural acidifying agent for lowering the pH of spray solutions and increasing penetration of tank-mixed materials. Use of BioLink Buffer and Penetrant reduces pesticide and fertilizer degradation caused by alkaline hydrolysis. It does not use corrosive mineral acids and is safe to use in spray equipment.
This entry was posted in Organic Products, Pesticides and Adjuvants, Products by admin. Bookmark the permalink.


College Credit Card Usage Statistics.
Media helps get the word out about getting "good credit"
It is a very exciting time for United College Marketing Services. For 17 years, we have positively affected the credit card marketing industry by developing innovative sales methodologies that credit educate people in the process.
We have been richly rewarded as we are one of the most profitable companies in our field. In addition to measuring up well financially we have also made quantifiable improvements in the lives of the college students we sell to, the campus administrators we work for and the card issuers that rely on us for growth. Through our Campus Merchant Program, we are able to pass some great savings unto the students while at the same time providing campus merchants a channel with which to reach their target consumers, students.
I personally want to thank all those that have partnered with us in this journey. Senator Ted Kennedy from Massachusetts and Representative Carolyn Maloney from New York for making the nation more aware of credit card marketing issues. Key people in the press such as Robert Heady from Bank Rate Monitor, David Robertson from Nilson Report and Jim Daly from Credit Card News have been very gracious in tracking our progress.
United College Marketing Services is a company that promotes credit cards to college students. Instead of the typical sales approach, UCMS avoids solicitory methods. We try to offer students more value. An example of this is Credit Strategies Seminars. Just about anyone can set up a booth to sell cards on campus, so UCMS offers this alternative.
We started offering Credit Strategies Seminars to combat the people that screamed "Get good credit!" but did nothing to explain what good credit really is. We have developed Credit Strategies Seminars to be of use to you, the college student. In one section we actually show a scorecard that shows how a loan officer evaluates applicants. We also give you an address that allows for you to get transaction recorders FREE!
We also offer a Campus Merchant Program which is a locally based, premium discount coupon program that is specific for each college that we market at. Discounts range between $100 to $250 per coupon book. Student users receive the book entirely free of charge so long as they have a visa/mc card. Users are not required to use the visa/mc card. The CMP has received much positive press because it saves college students extra money over normal campus coupon books. Since 1989, we have saved 7,500,000 students 2.4 billion dollars. UCMS has the goal of saving students 3.25 billion dollars by 2008!
We have interview tape, b-roll and company executives available to give interviews.
• "College-Why Issuers Are Targeting America's Youth"
• "Credit Where Credit Is Due?"
• "College Credit Cards Outlawed? How NY and Mass Might Curb Marketers"
• "Credit Education 101: A Sales Pitch With a Plus"
• "Issuers Face a Visit To The Dean's Office"
• "Students See Red Carpet Treatment From Banks"
• "Negotiate Your Way To A Lower Rate Credit Card"
• "Avoid Common Pitfalls That Plague College Students"
• "Turn That Piece Of Plastic Into A Financial Tool"
• "Learn What Credit Industry Insiders Want To Keep Secret"
© 1989 - 2014 UCMS. All rights reserved.


the cutesy name for the uterus.
when im on my period, I get crampies in my uteroo.
Looking for uteroo meaning? Delighted that you discovered the greatest determine on our web site!
Recently this definition was actually observed 51 times.


text:
Q: Does Curity Introspection supports application/token-introspection+jwt? Does anyone know if Curity supports application/token-introspection+jwt?
I am trying to implement https://curity.io/resources/learn/introspect-with-phantom-token/ and according to https://connect2id.com/products/server/docs/api/token-introspection
application/token-introspection+jwt seems to be the one we should be using.
However, when I tried to use it, I am getting a HTML page with below message.
Provided content type not in list of allowed ones [application/json, application/jwt]

A: The specification that introduce application/token-introspection+jwt is in draft state and not yet supported by Curity. Similar (more or less same) functionality can be achieved by using Accept: application/jwt instead.

redpajama_set_name: RedPajamaStackExchange


text:
package resourceread

import (
	storagev1 "k8s.io/api/storage/v1"
	"k8s.io/apimachinery/pkg/runtime"
	"k8s.io/apimachinery/pkg/runtime/serializer"
)

var (
	storageScheme = runtime.NewScheme()
	storageCodecs = serializer.NewCodecFactory(storageScheme)
)

func init() {
	if err := storagev1.AddToScheme(storageScheme); err != nil {
		panic(err)
	}
}

func ReadStorageClassV1OrDie(objBytes []byte) *storagev1.StorageClass {
	requiredObj, err := runtime.Decode(storageCodecs.UniversalDecoder(storagev1.SchemeGroupVersion), objBytes)
	if err != nil {
		panic(err)
	}
	return requiredObj.(*storagev1.StorageClass)
}

redpajama_set_name: RedPajamaGithub


text:
paul cram
StarShipSofa No 451 Tony Pi and Costi Gurgu
September 14, 2016 by Jeremy Szal
Main Fiction: "Cosmobotica" by Tony Pi and Costi Gurgu
Originally published in The Mammoth Book of Dieselpunk
Tony Pi is a Chinese–Canadian writer in Toronto with a Ph.D. in Linguistics. He is the winner of the 2015 Aurora Award for Best English Poem/Song, a multiple past finalist in the category of Best English Short Fiction, and finalist for the 2009 John W. Campbell Award for Best New Writer. His work appears in many anthologies and magazines, including Clarkesworld Magazine, Fantasy Magazine, Beneath Ceaseless Skies, and The Time Traveller's Almanac.
Costi Gurgu was born in Constanta, the 2600-year-old Greek city on the Black Sea shore, and lives in Toronto with his wife, on the Ontario Lake shore. Large bodies of water help Costi glimpse into other realms. That and some Dacian magic. His fiction has appeared in Canada, the United States, England, Denmark, Hungary and Romania. He has sold three books and over fifty stories for which he has won twenty-four awards. His latest sales include the anthologies "Ages of Wonder", "The Third Science Fiction Megapack", "Tesseracts 17", "The Mammoth Book of Dieselpunk", "Street Magick-Tales of Urban Fantasy" and "Dark Horizons". His novel "RecipeArium" will be published in 2016 by White Cat Publications.
Narrated by: Paul Cram
Paul Cram's young-with-a-touch-of-gravel voice is a bit newer to the world of audio than it is to other acting forms. Listening fans will hopefully be excited by several upcoming audio book titles in the next year being voiced by him. You can listen to his audio books that are currently available right now through Amazon, iTunes, and Audible.
Cram was most recently seen on set for the feature film WILSON opposite Woody Harrelson, and the indie film ANNIVERSARY shot in Maine, USA by movie director Jim Cole.
When not on a movie set or in a recording booth, Paul can be found deep-frying chicken wings with his sister in her kitchen, or quarreling about pop-culture with his little brother around one the 10,000 lakes of Minnesota. You can find his website Paul Cram Actor or IMDB.
Also, be sure to sign up for Paul's monthly newsletter loaded with contest prizes, movie releases, and of course audio book narration details as they become available.
Fact: Looking Back at Genre History by Amy H. Sturgis
http://rss.acast.com/starshipsofa/starshipsofano451tonypiandcostigurgu/media.mp3
Categories: Podcast | Tags: actor narrator, canadian author, costi gurgu, dieselpunk, mammoth book of dieselpunk, paul cram, paul cram actor, tony pi, WOTF winner
StarShipSofa No 425 Bruce Sterling and Jill Heinerth
March 2, 2016 by Jeremy Szal
Interview: Cave diver Jill Heinerth explores hidden underground waterways running through our planet.
Main Fiction: "Black Swan" by Bruce Sterling
Originally published in Interzone, reprinted in Years Best SF 15.
Bruce Sterling, author, journalist, editor, and critic, was born in 1954. Best known for his ten science fiction novels, he also writes short stories, book reviews, design criticism, opinion columns, and introductions for books ranging from Ernst Juenger to Jules Verne. His nonfiction works include THE HACKER CRACKDOWN: LAW AND DISORDER ON THE ELECTRONIC FRONTIER (1992), TOMORROW NOW: ENVISIONING THE NEXT FIFTY YEARS (2003), and SHAPING THINGS (2005).
He is a contributing editor of WIRED magazine and writes a weblog. During 2005, he was the "Visionary in Residence" at Art Center College of Design in Pasadena. In 2008 he was the Guest Curator for the Share Festival of Digital Art and Culture in Torino, Italy, and the Visionary in Residence at the Sandberg Instituut in Amsterdam. In 2011 he returned to Art Center as "Visionary in Residence" to run a special project on Augmented Reality. He has appeared in ABC's Nightline, BBC's The Late Show, CBC's Morningside, on MTV and TechTV, and in Time, Newsweek, The Wall Street Journal, the New York Times, Fortune, Nature, I.D., Metropolis, Technology Review, Der Spiegel, La Stampa, La Repubblica, and many other venues
Discuss this episode on our forums.
http://rss.acast.com/starshipsofa/starshipsofa-no-425-bruce-sterling-and-jill-heinerth/media.mp3
Categories: Podcast | Tags: actor, anniversary, bruce sterling, cave diving, cyberpunk, hard sf, interzone, itunes, jill heinerth, jim cole, nature, paul cram, paul cram actor, woody harrelson


text:
Shared Resilience: Businesses Are Ready to Partner to Build Climate Resilience
Samantha Harris, Manager, BSR
Follow @h@Samantha_Lacey
#Climate Change,
This past weekend at COP23, the We Mean Business coalition cohosted the 15th annual Development and Climate Days—the signature climate development and resilience event during the UN climate negotiations. We Mean Business brought together resilience experts from around the globe, including business leaders, to discuss innovative ways to enhance community resilience in relation to climate change.
One of the themes during this event focused on "shared resilience," which embodies the concept that no one actor can tackle this complex issue alone: We must work together. Climate risk is no longer thought of as something only impacting small island nations, but something that affects businesses, the global economy, and developed nations. We have seen this in particular this year, with natural disasters in the United States and extreme weather events around the world, such as the flooding in South Asia. All stakeholders must collectively work to create successful, lasting partnerships and initiatives that boost climate resilience.
For business, climate change is a material risk. Over the past three years, the World Economic Forum's Global Risk Report ranked climate change as the highest risk facing business. Companies, therefore, have a crucial role to play in both building their own adaptive capacities to climate-related events and enhancing broader societal resilience.
Throughout the Development and Climate Days at COP23, we were lucky to hear from several multinational companies, along with other experts, that are already working to address climate resilience—some are doing so because resilience, development, and risk management are not fundamentally different. All three activities demand systems thinking, identifying root causes of vulnerability, long-term planning, and taking practical action.
Business already assesses many types of risk through enterprise risk management systems, and companies can consider applying an environmental and climate lens to these existing assessments. Holistic and comprehensive resilience strategies are the best bet to address climate risks within the operations, supply chains, and vulnerable communities in which business operates.
Companies can begin by assessing climate risk; they can then develop a resilience strategy that includes their physical, financial, social, natural, human, and political assets. One example of a company that is doing this is Woolworths Holdings Limited, which is tackling climate risk throughout its supply chain and within vulnerable communities through its people. With a program that seeks to enhance the livelihoods of local women throughout its supply chain, Woolworths works with smallholder farmers on water management and farming techniques.
The Kellogg Company also helps farmers in different places face challenges by addressing access to market issues, making supply chain pricing more transparent, and sharing growing and storing techniques.
Finance is also key to the bigger picture of how we'll transition to a resilient society. How do we increase scale, broaden financial flows, and expand access to finance to those who need it to adapt to the impacts of climate change? Applying financial capital isn't only about matching funds with project pipelines. It will require collaboration among companies, banks, and all levels of government. Capital can finance non-financial instruments, too, such as procurement, government engagement, guaranteed offtake, and disclosure. These tools can help move the trillions in the real economy necessary to combat climate change.
Companies are ready to partner on the issue of climate resilience. In this spirit, Michelle Patron, director of sustainability policy at Microsoft, told participants of Development and Climate Days, "We want to partner with you and understand where the challenges are—and get tools into the hands of the people who need them most."
Samantha (Sam) drives the climate resilience pillar of BSR's climate change practice, which includes how businesses can act at the intersection of people and climate change. She leads BSR's foundational research and strategy on the intersection of climate and women's empowerment, as well as this cluster in the Business Action for Women collaboration. Sam also leads the international policy work at the… Read More
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