text:
< Return to listing
Lawrence Lash, Ph.D.
Professor and Associate Chair
L.H.LASH@wayne.edu
540 East Canfield Avenue
Office Fax
Dr. Lash's research program over the past nearly three decades has focused on various aspects of determining how chemicals produce injury to the kidneys and how we can design approaches to preventing or correcting such injury. The chemicals that we have used to produce kidney toxicity fall into one of three categories: 1) Model chemicals that are used to study specific mechanisms of action; 2) pharmacologic agents, such as analgesics or antibiotics; and 3) environmental chemicals, such are trichloroethylene and perchloroethylene. We have also explored the impact of chronic diseases (e.g., diabetes) and pathological states (e.g., reduced nephron mass with compensatory renal hypertrophy) on susceptibility of the kidneys to chemically induced toxicity. Although some of our studies have been conducted in intact, experimental animals, most of our studies have involved a variety of in vitro systems, including freshly isolated and primary cultures of kidney cells from rats and humans, subcellular fractions, or purified proteins. These types of in vitro model systems have afforded us the opportunity to dissect biochemical and molecular mechanisms of action, to manipulate and specify incubation conditions and to validate them in terms of functional integrity and relevance to the normal, in vivo state.
Key research accomplishments:
Discovery and characterization of a sodium-coupled GSH transporter on renal basolateral membrane that accounts for most of the clearance of GSH in the renal circulation.
Identification of mitochondria as a major subcellular site of action for nephrotoxic cysteine S-conjugates of halogenated solvents, such as trichloroethylene, in rat and human proximal tubular cells.
Development of in vitro procedures to isolate cell suspensions from proximal and distal tubular regions of the rat nephron, to enable investigation of factors that determine cell type specificity of susceptibility to pathological and chemically induced injury.
Characterization of sex- and species-dependent differences in metabolism and sensitivity to trichloroethylene and perchloroethylene, providing data for human health risk assessment.
Identification of the dicarboxylate carrier (DIC; Slc25a10) and the oxoglutarate carrier (OGC; Slc25a11) of the mitochondrial inner membrane as being responsible for transport of cytoplasmic GSH into renal and hepatic mitochondria.
Characterized protein expression and transport function of a battery of organic anion, organic cation, and amino acid transporters and expression of Phase I and Phase II drug metabolism enzymes in primary cultures of human proximal tubular cells, validating these cells for use in drug development.
Demonstrated adaptive changes in mitochondrial GSH transport and redox status in proximal tubular cells from kidney of diabetic or uninephrectomized rats.
Current Research Directions
Mitochondrial Redox Status in Diabetic Nephropathy
Our overall goals are to increase understanding of the underlying biochemistry of the diabetic renal proximal tubule and ultimately develop a novel therapeutic approach. To accomplish this, we focus primarily on what is considered the primary source of cellular dysfunction, the mitochondria, and on modulation of mitochondrial GSH status. Studies will use renal tissue and primary cultures of PT cells from streptozotocin (STZ)-induced diabetic and age-matched control Sprague-Dawley rats as a Type 1 diabetes model, and Zucker obese diabetic and Zucker lean control rats as a Type 2 diabetes model. We will test the hypothesis that overexpression of mitochondrial GSH transporters in proximal tubular cells of diabetic rats reverts the cells to a normal phenotype.
We will test the hypothesis that overexpression of mitochondrial GSH transporters in proximal tubular cells of diabetic rats reverts the cells to a normal phenotype. We hypothesize that overexpression of the DIC and/or the OGC in primary cultures of renal PT cells from diabetic rats can produce a sustained improvement in mitochondrial function and effectively convert the cellular phenotype to that of the normal PT cell. We will then test the hypothesis that modification of specific proteins and signaling pathways are associated with the diabetic nephropathy phenotype and with reversion of the phenotype in cells overexpressing mitochondrial GSH transporters. We propose to identify specific proteins, largely mitochondrial in origin, that either undergo covalent or oxidative modification or are altered in abundance in renal PT cells as a consequence of diabetic nephropathy and that are responsive to overexpression of mitochondrial GSH transporters.
Renal Mitochondria as Sentinels for Environmental Exposures
Our overall hypothesis is that renal mitochondria can serve as sentinels for exposures to certain environmental toxicants and that both proteins and low-molecular-weight compounds are released from renal cells and can be used as sensitive biomarkers. We propose that changes in either proteins (abundance of or unique modifications in key mitochondrial proteins) or unique mitochondrial metabolites will occur in response to exposures to nephrotoxic chemicals that target renal mitochondria and that these will be released into the tubular lumen and ultimately secreted in urine. Two biological models will be used: 1) Primary cultures of proximal tubular cells from human and rat kidney (hPT and rPT) for discovery and mechanistic studies and 2) the male F344 rat for in vivo validation. Two classes of chemicals will be studied: 1) Cysteine conjugates that target renal mitochondria and are derived from nephrotoxic halogenated solvents, namely S-(1,2-dichlorovinyl)-L-cysteine (DCVC; penultimate nephrotoxic metabolite of trichloroethylene [TRI]) and 1,2,3,4,4-pentachloro-1,3-butadienyl-L-cysteine (PCBC; penultimate nephrotoxic metabolite of hexachloro-1,3-butadiene [HCBD]); and 2) two model toxicants, tert-butyl hydroperoxide (tBH) and methyl vinyl ketone (MVK), which cause renal mitochondrial toxicity and exemplify the two distinct mechanisms of oxidative stress induction by the cysteine conjugates, namely generation of reactive oxygen species (ROS) with lipid peroxidation (i.e., tBH) and thiol alkylation (i.e., MVK).
Redox Therapy to Treat Environmentally-Induced Kidney Cancer
Our overall hypothesis is that mitochondrial redox status is an appropriate therapeutic target for treatment of kidney cancer induced by exposure to the environmental contaminant trichloroethylene (TRI). Mitochondrial dysfunction is widely believed to be an underlying factor in numerous diseases and pathological states, including many cancers. Furthermore, oxidative stress is known to be an underlying mechanism that mediates this dysfunction. We propose that overexpression of mitochondrial GSH transporters in PT cells from human kidney will produce a sustained improvement in mitochondrial and cellular redox status, thereby correcting or inhibiting the deleterious effects of TRI.
Polymyxin Antibiotic Nephrotoxicity
Two research collaborations are underway to improve the therapeutic efficacy and safety of polymyxin antibiotics. One project involves collaboration with medicinal chemists to develop novel polymyxin derivatives in which efficacy is improved while adverse side effects (i.e., nephrotoxicity) are diminished. Primary cultures of hPT cells will be used to screen for nephrotoxicity. The other project involves modulation of renal transport to minimize renal accumulation of and toxicity induced by polymyxins, thereby enhancing their therapeutic efficacy.
Baccalaureate: Case Western Reserve University, Cleveland, Ohio
Degree: B.A., Biology and French; granted May, 1980
Dates of Attendance: August, 1976 to May, 1980
Graduate:	Emory University, Atlanta, Georgia
Degree: Ph.D., Biochemistry; granted May, 1985
Dates of Attendance: September, 1980 to December, 1984
Dissertation: Functions of the basal-lateral membrane in thiol metabolism
Ph.D. Advisor: Dr. Dean P. Jones
University of Rochester, Rochester, New York
Postdoctoral Fellow, Department of Pharmacology and Division of Toxicology, Department of Radiation Biology and Biophysics
Dates of Appointment: January, 1985 to October, 1986
Postdoctoral Advisor: Dr. M.W. Anders
In vitro methods in renal toxicology
Primary cell culture
Drug metabolism and transport assays
Glutathione in kidney function and toxicity
Mechanisms of acute and chronic cytotoxicity of environmental chemicals in human kidney
Development of biomarkers of environmental chemical-induced nephrotoxicity
Prior Appointments
Instructor, Department of Pharmacology
Dates of Appointment: October, 1986 to February, 1988
Wayne State University, Detroit, Michigan
Assistant Professor, Department of Pharmacology
Dates of Appointment: March, 1988 to August, 1994
Member, NIEHS Center for Molecular Toxicology with Human Applications
Dates of Appointment: August, 1989 to March, 2010
Associate Professor, Department of Pharmacology
Dates of Appointment: August, 1994 to August, 2001
Associate Professor with tenure, Department of Pharmacology
Marshall University, Huntington, WV
Associate Member (DS), Graduate Faculty
Dates of Appointment: August 1, 1999 to September 30, 2006
J.A. Cichocki, K.Z. Guyton, N. Guha, W.A. Chiu, I. Rusyn, and L.H. Lash: Target organ metabolism, toxicity, and mechanisms of trichloroethylene and perchloroethylene: Key similarities, differences, and data gaps. J. Pharmacol. Exp. Ther. 359, 110-123 (2016). [PMID: 27511820]
A. Gallardo-Godoy, C. Muldoon, B. Becker, A. Elliott, L.H. Lash, J. Huang, M. Butler, R. Pelingon, A. Kavanagh, S. Ramu, W. Phetsang, M. Blaskovich, and M. Cooper: Activity and predicted nephrotoxicity of synthetic antibiotics based on polymyxin B. J. Med. Chem. 59, 1068-1077 (2016). [PMID: 26734854]
L.H. Lash: Mitochondrial glutathione in diabetic nephropathy. J. Clin. Med.4, 1428-1447 (2015). [PMID: 26239684]
J.X. Huang, G. Kaelsin, M.V. Ranall, M.A. Blaskovich, B. Becker, M.S. Butler, M.H. Little, L.H. Lash, and M.A. Cooper: Evaluation of biomarkers for in vitro prediction of drug-induced nephrotoxicity: comparison of HK-2, immortalized human proximal tubule epithelial, and primary cultures of human proximal tubular cells. Pharmacol. Res. Perspec. 3(3), 2015, e00148 (2015). [PMID: 26171227]
L.H. Lash, D.A. Putt, and B. Benipal: Multigenerational study of chemically induced cytotoxicity and proliferation in cultures of human proximal tubular cells. Int. J. Mol. Sci. 15, 21348-21365 (2014). [PMID: 25411799]
Search PubMed for publications from the Lash Lab
Faculty Status
Cell Biology, Toxicology
facebook twitter instagram flickr youtube
6374 Scott Hall
540 E Canfield
Detroit, MI 48201 | (map)


Here at Buchanan Chiropractic Clinic, PC, we have many different services to fit your specific needs. Whether you are experiencing back pain, feeling out of alignment, or are just looking to improve your health, we can help.
Learn more about it in this video.
We offer a wide range of therapies including EMS, ultrasound, heat therapy amd laser.
Chronic back pain is one of the biggest problems Americans face today. It affects eight out of ten people at some point in their lives. Even though back pain is common, the level of severity varies. A variety of different conditions are responsible for discomfort in different regions of your back. These include sprained ligaments, strained muscles, trigger points, arthritis, ruptured disks, inflamed joints, sport injuries, and many more. We will locate the source and do everything we can to ease the uncomfortable pain you may be experiencing.
An auto accident has the ability to tweak your neck, your spine, or even force your entire body out of proper alignment. Our Doctor is ready to locate the source of each issue and prescribe the necessary adjustments and corrective exercises as they see fit. Our goal is to have you feeling like yourself again in no time.
We provide specific recommendations on nutritional supplements and healthy food choices to enhance our patients return to optimal health. Do you know which supplements and vitamins are good for you? Dr. Buchanan can provide a structured nutritional program based on your individual needs.
These are just some of the services that we provide here at Buchanan Chiropractic Clinic, PC. We love providing our patients with an enjoyable and pain-free life, so don't hesitate to stop on by!
If you have any questions about the services that we provide our patients, please contact us today at (770) 474-6680.


Branding is the key to success.
In fact, Forbes put it this way, "Brands are psychology and science brought together as a promise mark. Products have life cycles. Brands outlive products."
Yes, your branding can either make or break your venture. And your branding all begins with a simple logo.
Whether it's on a ginormous billboard or a profile picture on an Instagram account, it's clear to see that the easiest way to recognize a company is through its logo.
However, there really is no "one size fits all" when it comes to logo size. How can you make sure your logo is looking its best in all different shapes and sizes? Read on to find out.
The master file for your logo should be created in a vector program so that your design can be scaled up or down accordingly.
Your logo should have at least two versions, horizontal and vertical.
When it comes to online profiles, your logo image size should be smaller than 100 KB if possible.
As mentioned in the basic logo rules above, a vector is the best format to have your logo designed in. Raster graphics (or bitmaps) are another option, but they can actually limit reproduction.
A vector graphic is made up of precise points which ensure consistency throughout a variety of sizes. The logo can usually be scaled to any size without losing any quality, which makes late edits much easier to achieve.
Raster images can be created in large formats, but you never know how large you will need to reproduce your logo in the future. If it's blown up to a huge size, it will no doubt look pixelated and lose its professional look.
Overly complex designs don't scale well when they are printed or are used as tiny icons (for example your favicon icon). Complicated bad logos may just look like a blob of nothingness.
The more details a logo has, the more information the viewer has to think about and process. If you recall the most memorable and successful logos throughout history, most of them are super simple.
For example, McDonald's, Apple, Nike, FedEx, or even Batman! Their icons can be resized upwards and downwards without losing any vital details.
Logos Here, There, and Everywhere!
In this technical age that we live in, logos aren't just found on the door of your office. From your website and facebook account to your office stationery, your logo and branding will be literally everywhere.
Read these next 3 size guides on what the specific logo dimensions should be for each avenue of exposure. Please note that all the logo dimensions mentioned are in pixels and in order of width by height.
Once your logo design is ready, it will need to be uploaded to your website. Generally, the optimal logo dimensions for a webpage are 250 x 150.
You also need to bear in mind your favicon which is a tiny iconic image that will represent your website or business. Favicons are often found in your web browser address bar. They can also be used in bookmarks and feed aggregators too.
Even though it's small, it's mighty. The aim of your tiny favicon should be to identify your brand at a glance. You could feature your full logo if it's simple enough, the first letter of your brand name or the image from your logo.
Using the optimal logo size on your social media pages is vital. If your logos aren't matching the recommended dimensions they could either be stretched and grainy or look like a squashed compressed logo.
However, the optimal logo dimension for most social media pages in 1024 x 512 pixels. Your logo will display correctly across most social media platforms. But there could still be a few issues, so it's always good to follow the above logo size guide.
What Logo Size for Video Productions?
Video media is seen on many different devices and locations like hand-size smartphones, laptop screens, tablets or larger cinematic projections. Therefore no size fits it all. If your logo size looks good on your laptop screen, it could be way to small for displaying via social media on a smartphone.
So whenever you need to define a logo size for your video, the rule of thumb is to think first about "where" and on "what device" your main target audience will enjoy the video content. If you aim for social media on mobile devices, make your logo 10% bigger, if your aim are laptop screens or TV screens, make your logo a bit smaller.
Tip: Most video productions don't have the ability to adjust the logo size to individual formats, so it would be wise to use an average size for the logo that looks reasonable on most video playback devices. Try playing your video in full-screen mode on your laptop and smartphone, if your logo looks good and easy-to-read on both devices, congratulations you found a good logo size.
When it comes to printing your logo, more than the size, the format is important. As mentioned before, the best logo formats should be in vector and not raster. This will make it easy to edit when the need arises, especially when printing.
It's also key to design your logo so that it's adaptable. For instance, it should be fluid enough to switch between vertical and horizontal layouts without it being an issue. This is vital for printing as shapes and sizes can vary dramatically (hats, t-shirts, pens, car wraps, and more).
If you have your logo saved in a vector format, then the size won't matter. But if you do have it in a raster image, you should ensure that the size is as big as possible.
How Can I Resize a Logo?
You may realize that you need to start resizing a logo asap. The options include using photoshop or something similar to edit the dimensions DIY style. This option is only really acceptable if you have some kind of experience, or you could make a huge mess of it.
Another option is by commissioning a freelance designer or a reliable contractor. A freelance designer will be cheaper, but a contractor can cover all your bases if you're not strapped for cash.
Logo Size Sorted, What's Next?
Now, you're informed about which logo size you need for each avenue of branding. But, with logos evolving rapidly, it's sometimes hard to keep up with the latest tech fashions.
A really cool tech trend that's updating even the most boring looking websites is using moving logos. Read more about this awesome trend here.


← Mathematical Methods for Optical Physics and Engineering on Amazon!
Chimpanzees Prefer Fair Play To Reaping An Unjust Reward. We're often told that "life isn't fair", but certain of our primate relatives feel otherwise: Eric Michael Johnson in The Primate Diaries describes research that demonstrates that chimpanzees have a good sense of fair play!
A Cataclysmic Variable in the Field of the Kepler Mission. At his eponymous blog, Professor Astronomy describes his personal astronomical observation of a "cataclysmic variable" — and explains what that means!


It was soon after some ideal New from this plastic new members showed up that some free organizations began to watch a prompt diminishment. Regardless of changing supplier to benefit as much as possible from progressively moderate costs most currently end up back utilizing their past merchant or among those significant 6. All these considerable 6 have a blended piece of the all out segment of more than 96 percent in the consequence of eating the new competitors in essentially undisputed takeovers. Roughly 2/3 of those associations are troublesome by German or French multi-nationals once in a while the solid contenders the focal experts announced would happen because of de-course from the 1990s. Perhaps if the specialists had wandered in to keep a vital space from the very same out of having the period and scattering of electricity things at that point we'd have seen substantially less heart consideration.
And furthermore neighborhood Company is you're most defenseless against electricity customers. Significant private venture customers can utilize their motivating strong tissue to check far more prominent costs. Family customers are forcefully guaranteed by the authoritative pros. Private venders are will surely scatter rates alongside the control viably encourages the buyer to see free and impartial cost recognize sites so as to find the perfect game plan. It's in like manner simple for your family unit client to adjust provider at whatever arrange they, by method for example, Not for your minor association who dependably discovers rate connections troublesome in light of the fact that most of the way into the cerebrum boggling supply number edge anyway in addition into the usually climbing and diminishing rebate costs experienced under present budgetary situations. You just don't have enough capacity to check at a citation from the fear it might change totally from the following day.
Presently there's the vidējais elektrības patēriņš mājsaimniecībā. The electricity value chance to motivate a present supplier association to Switch is truly confined. Tangling the noteworthiness framework better are the various techniques that a supplier can refer to. Some want to fill the Standing expense while some pack the edge cost. On The off likelihood that you are setting serenely on a noteworthy supply of this Industrial focus making to some awesome degree gigantic benefit there isn't any rousing power to be lean. You will know about what position I am keeping and snap to see more.


If you are stopped by police on the road in the state of Hawaii and you have no proof of auto insurance, you will get penalties. You can get fine $1,000 for the first offense and even till $3,000 for the next offense. Besides that license's suspension also is one of thing that possible to happen if you don't carry the proof of auto insurance coverage. And the final offense you are able to enter jail in times. Those are which as the background of people in the state of Hawaii looking for the right coverage and affordable auto insurance to choose.
There are many companies in Hawaii which offer auto insurance coverage, but sometimes it's not easy for you finding the right one. Considering about the right company indeed is one of important thing should be considered so that you can get the best coverage. It'll be a good consideration and most people's requirement for getting the affordable auto insurance companies in Hawaii. There are some companies which qualify for it depending on some characteristic and what we are looking for. For instance, if you desire finding the cheapest auto insurance, you need to choose company which offers it.
Besides that you can compare from one to another company available there, but the good thing for you to consider about the state law first. The state of Hawaii requires similar with another requirement, liability is one basic coverage should be had. These are minimum rate of level $20,000 for single injury or death, $40,000 for total injury or death, $10,000 for property damage liability and $10,000 for property damage liability. These are important for people who are looking for auto insurance coverage in Hawaii. Should be keep in mind that auto insurance is a mandatory and it's not as an option.


text:
nova página do texto(beta)
Rev. Soc. Bras. Med. Trop. vol.52 Uberaba 2019 Epub 30-Maio-2019
Antifungal efficiency of chemically and thermally-activated acrylic resins after surface treatment using poly (diallyldimethylammonium chloride)
Rennan Luiz Oliveira dos Santos1
Carina Domaneschi1
Victhoria Beatriz Pedroso1
Giovanna Piacenza Florezi1
Luciana da Silva Ruiz2
Rinaldo Ferreira Gandra3
Norberto Nobuo Sugaya1
Claudete Rodrigues Paula1
1Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brasil.
2Instituto Adolf Lutz-CLRII, Núcleo de Ciências Biomédicas, Bauru, SP, Brasil.
3Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.
Acrylic resins are used in the preparation of facial prostheses and may be colonized by fungi. Here, we verified the antifungal efficacy of this material after surface treatment using poly (diallyldimethylammonium chloride).
Acrylic resin specimens with and without surface treatment were subjected to tests for fungistatic and fungicidal activities. Standard strains of Candida albicans and Aspergillus niger were used.
After surface treatment, the fungistatic and fungicidal efficacies of the resins against C. albicans and fungistatic action against A. niger were verified.
The surface treatment was a determinant of the antifungal activity of the material.
Keywords: Acrylic resins; Biocide; Dental materials
Acrylic resins are widely used for various applications due to their favorable characteristics, such as biocompatibility, ease of processing, polishing capacity, and low cost. In dentistry, these materials are used to rehabilitate facial and stomatognathic structures affected by pathologies, trauma or surgery, restore aesthetics and function, and assist in psychological therapies1,2.
However, the susceptibility of these resins to the adhesion of microorganisms, particularly in cases of poor patient hygiene, is the main cause of infections and inflammation of the area in contact with the prosthetic material3,4.
Given this situation, there is a worldwide trend of testing antimicrobial agents together with the dental and medical materials in order to avoid its colonization with bacteria and fungi5-8. The results, however, are quite varied and the antimicrobial mechanisms of some agents, such as poly (diallyldimethylammonium chloride) (PDADMAC), used for the surface treatment in dentistry, are still not fully understood,.
PDADMAC has quaternary ammonium salts in its structure and is classified as a biocide. Some of its advantages include low cost and biocompatibility and, currently, it is being used on a large scale for water purification9-11. In 2016, Silva et al. observed that these resins, upon surface treatment (TS), became efficacious against bacteria, but were not tested against fungi8.
Therefore, the present work aimed to evaluate the antifungal potential of the PDADMAC biocide when applied on the surface of a chemically activated acrylic resin (CAAR) and thermally activated acrylicresin (TAAR).
Round test samples measuring 3 cm in diameter were made with CAAR and TAAR of the Clássico® brand (São Paulo, SP). The TAAR was activated in long cycles at a low temperature (60ºC for 3 h and 70ºC for 9 h). The test specimens were separated into four groups as shown in the flowchart (Figure 1). Two of these groups were subjected to TS by the application of PDADMAC (Chemical Institute, University of São Paulo, Brazil) at concentration of 4% weight per 10 mL of tetrahydrofuran solvent (Labsynth, Diadema, SP). The drying time was set as ten minutes. The other groups did not undergo TS with the compound.
FIGURE 1: Flow chart demonstrating the preparation of test specimens. CAAR: Chemically activated acrylic resin; TAAR: Thermally activated acrylic resin; TS: Surface treatment.
Totally 48 test specimens were subjected to sterilization with gamma radiation at a dosage of 25 KGy12. A culture suspension of 100 µL Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404) at a concentration of 106 UFC were spread on the agar surface. Mycological tests were performed as per the protocols of the American Association for Testing and Materials for assessing the antifungal properties of the material against the standard strains using a Biorad brand spectrophotometer. The conidia and blastoconidia of A. niger and C. albicans were used to perform the tests.
The fungistatic property was verified by the G21-15 test13, where the test bodies were placed in contact with petri dishes containing Sabouraud dextrose agar (Sigma, USA) and the two fungi mentioned above. The results were recorded after 7, 14, and 28 days of incubation and depending on the extent of fungal growth, a visual classification score was established as follows: 0 (no growth), 1 (growth traits), 2 (mild growth), 3 (moderate growth), 4 (abundant growth). This test was performed in triplicates.
The fungicidal properties were analyzed by the E2149-1314method in which the fungal solution was cultured in 50 mL of phosphate buffer and the experimental materials were placed into it. This solution was collected at two different timepoints as follows: initial concentration of viable microorganisms (0 hours) and final concentration (1 hour) followed by determination of the percentage reduction of the colony forming units (CFU). The test was performed in triplicates.
The Shapiro-Wilk test was used to check the assumption of normality of the data distribution of the fungicidal tests. A Student's t test was used to observe the differences between the control and TS groups and the test for repeated measures was applied in order to observe the differences between the initial and final measurements of each of these groups. A significance level of p <0.05 was established.
The fungistatic tests G21-15 against C. albicans showed that the TS samples maintained a score of 0 at the three evaluation timepoints, demonstrating significant efficacy. Contrarily, the score of the control samples increased gradually, indicating that fungal growth was not prevented. The results were similar to those observed for A. niger. The efficacy of TS samples was significantly different from that of the control (Figure 2).
FIGURE 2: Intensity of fungal growth of Candida albicans and Aspergillus niger represented by the G21-15 test codes analyzed at three different times. CAAR: Chemically activated acrylic resin; TAAR: Thermally activated acrylic resin; TS: Surface treatment.
The fungicidal tests E2149-13 showed that the control samples were not able to reduce fungal growth. In the TS samples, a reduction of about 99.99% against C. albicans strains and 0% against A. niger strains (Table 1), was found for both the resins.
TABLE 1: Fungi Count for Candida albicans and Aspergillus niger.
Aspergillus niger
% of
p-values
reduction or increasing
CAAR 2.2×105 2.3×105 +0.02 - 0.0572 1.5×105 1.6×105 +0.05 - 0.1835
CAAR + TS 2.2×105 1.6×10 -4.16 99.99 <0.0001* 1.5×105 1.6×105 +0.05 - 0.0377
TAAR 2.2×105 2.5×105 +0.06 - 0.0634 1.5×105 1.8×105 +0.07 - 0.0153
TAAR + TS 2.2×105 1.4×10 -4.21 99.99 <0.0001* 1.5×105 1.8×105 +0.06 - 0.0198
CAAR: Chemically activated acrylic resin; TAAR: Thermally activated acrylic resin; TS: Surface treatment. *Statistically significance: p<0.05.
The antifungal effect in acrylic resins is widely evaluated in literature. Several antimicrobial agents have already been tested in dental and medical devices. Amal and Amani, in 20166, have shown that the addition of henna powder to the acrylic resin prosthesis may be effective in controlling the proliferation of C. albicans on its surface, but further studies are required to determine its physical properties. Jain in 20138 tested the application of delmopinol on the surface of the thermally activated acrylicresins and obtained a higher reduction in the adherence of C. albicans after contamination when compared to that before the application of delmopinol.
The effect of the PDADMAC biocide in acrylic resins has been described in a few studies. However, in these studies, it has not been used for dental applications and therefore the influence of different types of polymerization types of this material have not been considered.
Sanches in 201515evaluated the acrylic resin with PDADMAC using nanoparticles and positive results were observed with respect to its antifungal activity against C. albicans. However, in this study, a polymethylmethacrylate resin was synthesized together with PDADMAC. Similar to the study by Amal and Amani7, these studies have constructed a type of inclusion that can interfere with the physical properties of the material. Our study, however, did not require an evaluation of the physical and mechanical properties of the material since a surface treatment of the resins was carried out.
Additionally, the materials are biocompatible because there is no molecular interaction between the PDADMAC and the resins. However, this lack of interaction implies a limitation, since, in environments with secretion interferences such as saliva, the biocide could leach and consequently its antifungal properties could be lost. Thus, to understand the impact of these interferences, new studies are being conducted.
The surface treatment using PDADMAC was a determinant of the antifungal activity and this activity was not influenced by the type of resin treatment. Its action was proven in thermally activated acrylic resins and when it was chemically activated against C. albicans. Contrarily, only its fungistatic action was proven against the fungus A. niger.
The authors would like to thank for the funds provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).
1. Abdulrazzaq Naji As, Jafarzadeh Kashi Tb, Behroozibakhsh Mc, Hajizamani Hd, Habibzadeh Se. Recent Advances and Future Perspectives for Reinforcement of Poly (methylmethacrylate) Denture Base Materials: A Literature Review. J Dent Biomater. 2018;5(1):490-502. [ Links ]
2. Kumar S, Rajtilak G, Rajasekar V, Kumar M. Nasal prosthesis for patiente with xeroma pigmentosum. J Pharm Bioallied Sci. 2013;5(2):176-78. [ Links ]
3. Von Fraunhofer, Joseph G & Loewy Z. Factors involved in microbial colonization of oral prostheses. Gen Dent. 2009;57:136-44. [ Links ]
4. Pereira-Cenci T, Cury AA, Cenci MS, Rodrigues-Garcia RC. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont. 2007;20(3):308-10. [ Links ]
5. Sivakumar I, Arunachalam KS, Sajjan S, Ramaraju AV, Rao B, Kamaraj B. Incorporation of antimicrobial macromolecules in acrylic denture base resins: a research composition and update. J Prosthodont. 2014 Jun;23(4):284-90. doi:10.1111/jopr.12105. [ Links ]
6. Nawasrah A, AlNimr A, Ali AA. Antifungal Effect of Henna against Candida albicans Adhered to Acrylic Resin as a Possible Method for Prevention of Denture Stomatitis. Int J Environ Res Public Health. 2016 May 23;13(5). pii: E520. doi: 10.3390/ijerph13050520. [ Links ]
7. Jain D, Shakya P. Anin vitro study on effect of Delmopinol application on Candida albicans adherence on heat cured denture base acrylic resin: A thorough study. Indian J Dent. Res. 2013;24:645. doi: 10.4103/0970-9290.123423. [ Links ]
8. Silva VRM, Dos Santos RLO, Petri DFS, Dias RB, Coto NP. Use of biocides on the surface of materials for making bucomaxillofacial prostheses. Brazilian Journal of Oral and Maxillo facial Surgery. 2017;7:21-24. [ Links ]
9. Zhao X, Zhang Y. Bacteria-removing and Bactericidal Efficiencies of PDADMAC Composite Coagulants in Enhanced Coagulation Treatment. CLEAN - Soil, Air, Water. 2012;42(1):37-42. [ Links ]
10. Hauck TS, Ghazani AA, Chan WC. Assessing the effect of surface chemistry on gold nanorod uptake, toxicity, and gene expression in mammalian cells. Small. 2008 Jan;4(1):153-9. [ Links ]
11. Wang L, Jiang X, Ji Y, Bai R, Zhao Y, Wu X, Chen C. Surface chemistry of gold nanorods: origin of cell membrane damage and cytotoxicity. Nanoscale. 2013;5(18):8384-91. doi: 10.1039/c3nr01626a. [ Links ]
12. Laughlin WL, Boyd AW, Chadwich KH, Donald JC, Miler A. Dosimetry for radiation processing. London: Taylor and Francis; 1989. [ Links ]
13. ASTM G21-15, Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi, American Society for Testing and Materials, West Conshohocken, PA. 2019. [ Links ]
14. ASTM E2149-01, Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions, American Society for Testing and Materials, West Conshohocken, PA2019. [ Links ]
15. Sanches LM, Petri DF, de Melo Carrasco LD, Carmona-Ribeiro AM. The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate). J Nanobiotechnology. 2015;13:58. doi: 10.1186/s12951-015-0123-3. [ Links ]
Financial Support: The work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (33002010138P1) and Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/07684-3) in Brazil.
Recebido: 23 de Janeiro de 2019; Aceito: 29 de Março de 2019
Corresponding author: Rennan Luiz Oliveira dos Santos. e-mail:rennan_475@usp.br

redpajama_set_name: RedPajamaCommonCrawl


text:
The best team liquid results are yet to come
Since the League of Legends franchise took on the significance it's Today, everyone else has wanted to participate in their elite dome of teams and players, eSports championships such as these gather a group of followers of more than three million individuals who follow along each season Plus they create their fan groups for every single team, the players eventually become actors and ofcourse team liquid the sum of money involved becomes staggering.
Their lovers have grown because their performance has improved and they've won championships; in the regional stage, their numbers demonstrate it like an average team with fifty% of games won and fifty% of matches lost, at his best he has won two tournaments in 2019.
With the newest adjustments of team Liquid in his group of players he has high hopes of going farther in the 2020 season, they are already considered in the United States but their objective is still to achieve international fame, they have been preparing for success since 2015 when they started their professional task.
Be known for the total amount of money it invests in its own signings and also the constant fluctuations in its own players, this reality has led them to occupy an important place at the regional positions nevertheless the results are not yet seen in the games and the global qualifiers, the current team has great expectations concerning it, even however some changes happen to be playing on its platform.
This sounding shift refers to one of its own members with much more time around the Team, it really is Broxa, what's coming is yet to be viewed and confirmed, at the moment the spring season is going to start and the fans are prepared to participate in the major's LoL meetings that will attract new leading teams or will repeat the very same ones who for a long time have headed the contests leaving hardly any for other teams.
Tags: Liquid, team


Medium defender from Western Australia who has a penetrating kick and reads the game well. Greaves is composed with the ball in hand and makes good decisions providing defensive rebound. A hard-working player with good endurance who caught the eye during the NAB AFL Under-18 Championships.
Draft history: 2019 Rookie Draft selection (Hawthorn) No. 13.


text:
Q: Find Measure of Angle $E$
How do you solve for angle $YEP$
I have tried using $\sin$, $\cos$ and $\tan$ to solve but I can't.
Is there supposed to be a rule of $30,60,90$ triangle when the altitude is made?

A: In triangle $AEP$ , $AE=$$20\over {\cos A}$ In the big triangle, $AE=25\cos A$
Equating them, you get $\cos A = $$2\over {\sqrt{5}}$ .
Angle $YEP$ = $90^o - $angle $PEA$ = angle $A$

redpajama_set_name: RedPajamaStackExchange


The International Trade Administration's Enforcement and Compliance office is extending by 40 days all deadlines for E&C proceedings in light of the recent federal government shutdown. This extension applies to every proceeding before this office as of 28 January, including the following (but excluding court-ordered redeterminations).
In addition, for AD/CV duty orders and suspension agreements with December and January anniversary months, all requests for administrative reviews will be due by 28 February.


In Part I of this mammoth piece, I analyzed Quinn's relationship with the Cheerios, and power. Season 2 has shown us choosing to get her power back, for better or worse. Power is mostly equated to the "old" Quinn - the one that tortured Rachel Berry and had everything she ever wanted.
Quinn's kindness has been established. And now that she has chosen power again, she similarly must continue to choose kindness, or else the character development is lost. So what's the status of Quinn's kindness - and her friendships - in Season 2?
For better or worse, Quinn is somewhat of an isolated character. In general, I appreciate this attribute of Quinn's personality, but I do also like the idea of her interacting with others. It gives us some insight on how she's conducting herself socially, which is part of her character's huge change. Without Quinn having friends to interact with, it's significantly more difficult to track her development.
Quinn's oldest friends, within the context of the show, are Santana and Brittany. I touched a little bit on the Quinn-Santana dynamic in Part I, but I made the mistake of surmising that Quinn and Santana were never friends, which is definitely an oversight on my part. Confounded absolutes! It's more complicated than that, and unfortunately we don't get a lot of concrete Santana-Quinn interaction without any piss or vinegar. It's true that Santana's loyalty to Quinn runs deeper than one would think - she didn't rat out her pregnancy to Sue, yet Quinn ratted out Santana's summer surgery. Quinn also slept with the guy that Santana had been quasi-dating. The question of loyalty is certainly interesting with these two.
Regardless, Quinn and Santana can probably be easily classified as the tried-and-true, if somewhat superficial, distinction of "frienemies." Season 2 opened up their dynamic instantly, with the fight in "Audition," and then dropped it just as immediately. Remember in "Rocky Horror Glee Show," Quinn and Santana both played Magenta? What a lovely opportunity to explore the dynamic of two friends and rivals playing the same role. Magenta, Head Cheerio - a great parallel could have been used there. But instead, we got to see Mr. Schuester needlessly overturn the whole production so he could be Rocky, and Season 2's Quinn and Santana are relegated to sniping at and dancing with each other. Missed opportunities, party of OH-SO-MANY.
Brittany and Quinn are even more mysterious. Quinn seems to have a permanent "WTF" face in Brittany's company this season, but it'd be nice to see another dimension to the dynamic. I'm assuming Quinn is no exception to the "Brittany likes everyone" rule (aside from those few early episodes where Brittany was not quite Brittany yet) but as of yet it's largely unexplored. Brittany's unerring and daffy sweetness would either drive Quinn crazy or be exactly what she needs. Either way, I'm game.
All in all, Quinn's post-pregnancy relationship with her erstwhile sycophants is maddeningly uncharted, and for a set of characters frequently packaged up and delivered as a trio, they rarely interact in a meaningful way. Change this, writers!
Next on the list of Quinn's friendships is Mercedes. The writers brought Ms. Jones in during the Back 9 for Quinn to relate to on the topics of body insecurity and feeling like a minority. Needless to say, I think we all did a double take. While my initial reaction to Quinn and Mercedes was one of skepticism, I have come to appreciate their dynamic and accept it as something that does need to be continued, even if it was originally a bit out-of-nowhere. Mercedes and Quinn were a very real entity in "Home," "Laryngitis," and "Funk," and Quinn even requested Mercedes to be in the delivery room with her when she had Beth.
So far, in Season 2, the extent of their relationship amounts to occasionally sitting next to each other in Glee Club, and what's sad is that we consider this an accomplishment of continuity. Even though they may not seem to be able to relate to one another in their current (lack of) storylines, their friendship needs to be present. Mercedes opened up her home to Quinn in a time when the girl did not have many good things going for her. This relationship should not just disappear.
Speaking of relationships that should not disappear, we are now rounding the corner into perhaps my favorite dynamic the show has ever given us: Rachel and Quinn. It is perhaps a dangerous idea to get me ranting on these two, because I could write pages. PAGES, I tell you! Their relationship is genius in that it is tied inextricably into the show's construct and the two characters' arcs. YET, we find that after the first 13 episodes, their interactions are few, far between, and frankly, don't speak to the reasons why these two SHOULD be interacting in the first place.
Quinn and Rachel were initially designed to be enemies. Opposites. Cheerleader/loser. Popular girl/unpopular girl. Traditionally beautiful/unconventionally beautiful. They are each other's foils, and represent the core dynamic the show addresses - Quinn is the embodiment of the Cheerios, the cool kids, and Rachel is the embodiment of Glee Club, the losers. On paper, they should hate each other. But in one of the show's true brilliant moments, Rachel reached out to Quinn when she got pregnant, setting aside all past animosity, and made her feel accepted. This gesture itself is an embodiment of one of the show's main themes. What's not to like?
My favorite Quinn Fabray moment is the scene she shares with Rachel, and then Puck, in "Sectionals." I'll expound on the Puck part later - for now, let's discuss the Rachel portion. Rachel initially pursued Quinn with friendship, but her obsession with Finn led her to destroy his relationship with Quinn by telling him the secret of the baby's father. But in this wonderful scene of tension, silence, and yet so much meaning, it is Quinn who forgives her for the transgression. This is the moment where Quinn chooses kindness, which is key to her character. It's a beautiful scene. They are supposed to hate each other. They've both transgressed against the other, and yet... they don't hate each other.
What more, they actually complement each other. Beneath their superficial polarities, Rachel and Quinn are actually quite similar. Their character traits both deal with control and determination, and their arcs both speak to the negotiation of self vs. others. These factors simultaneously attract and repel one another, making for a fascinating dynamic. The fact of the matter is that Quinn and Rachel were designed to interact, and all signs at the end of "Sectionals" seemed to point towards this development.
Of course, the Back 9 was sorely lacking in this respect, and the pair's complete and utter dearth of screentime in these episodes makes me cranky to this day. Season 2 has stepped up, however, in that they've at least spoken to one another. "Brittany/Britney" finds Rachel asking Quinn for a favor in regards to Finn, which has got to be a touchy subject - and yet, Quinn obliged, in a scene we were unfortunately not privy to. "Duets" shows Rachel encouraging Quinn to sing with Sam and win the competition. However, this specific interaction, while lovely to see, has little relevance to what their dynamic should be at this stage.
No, the original direction for Quinn's and Rachel's characters seems to have been largely dropped or ignored, and so I go into every episode hoping things will turn around, only to get irrationally angry when Quinn says she wants to punch Rachel in the face. Way to go, writers. I love it when you completely undo perfectly good character development for no good reason other than throwaway jokes.
So, when it comes to Quinn and kindness, I want to see it manifest in the form of gratitude to Glee - Mercedes and Rachel in particular. We got a snippet of this in "Duets," when she tells Sam she sticks with Glee because they've been good to her; and I won't lie, I cheered when she said it. If I ran the show, I would push for the construct that Quinn is terribly protective of Glee Club out of thankfulness, and that she fights back when the Glee Club is threatened. But alas, I do not run the show, and instead Quinn toes the line when it comes to acting out against her popularity.
Regardless, I still want some actual positive interaction between the people that Quinn has held tenuous friendships with, and perhaps even a storyline that allows for this to be developed simultaneously. Season 2 so far is generally on the right track, but still lacking in the character specifics. Quinn is capable of kindness, and even in her solitude, I want the writers to actively demonstrate that.
Your tribute to Quinn's and Rachel's fascinating relationship is the type of work that makes me want to be a writer. Amazing.
You're very welcome. I admire that you're confident enough to share your opinions and most important, you back them up with facts. It is something I work to achieve every day as a writer and as a person.
And you can't go wrong with Quinn and Rachel. I love both of them beyond belief.


Èze its a medieval village with a fabulous view over Saint-Jean-Cap-Ferrat. If you spend time at Cote d'Azur you might face only the luxury and the overpriced restaurants, hotels,…etc. The ancient village of Èze is different. On the top of the hill you can walk around in the botanic garden of the city, called Jardin Botanique d'Èze wich is known for its collection of cactus and for its wonderful panoramic view and you can also visit the ruins of the castle which was torn down in 1706.
This small medieval village has a lot of history but it is also famous for its beauty. It has many art galleries, nice restaurants and also two very beautiful five star hotel, both with an amazing view. I would recommend it for tourists and for honeymooners as well because of its charm and quietude.
If you would like to go from Hostel Smith you can take the bus 82 at Place Garibaldi which goes directly to Èze and only takes 20 minutes.


Christine Ohlman (a/k/a "The Beehive Queen" for her mile-high platinum-blonde hairdo) is the current, long-time vocalist with NBC's Saturday Night Live Band whose sixth critically-acclaimed CD with Christine Ohlman & Rebel Montez, "The Deep End," was honored on numerous national end-of-year Top Ten lists. She'll be joined onstage by the members of Christine Ohlman & Rebel Montez, including acclaimed international area guitar ace Chris Bickley; Michael Colbath (bass); and Boston drum god Lorne Entress.
Ohlman, born in the Bronx, has long and deep ties to New York both through her long, continuing tenure at SNL and via collaborations with Marshall Crenshaw, Willie Nile (she partnered with Crenshaw and Nile on a recent sold-out tribute to songwriter Doc Pomus at the Guthrie Center), GE Smith, Liberty DeVitto, Ricky Byrd, Eric "Roscoe" Ambel, Paul Shaffer, Ronnie Spector, Will Lee, John Leventhal and a host of others. She led the finale at Little Kids Rock's annual gala at the Sony Playstation theatre with Trombone Shorty, Jon Batiste and Lake Street Dive, raising more than a million dollars in a single evening.


Trump's budget proposal would hurt Americans and help the president's wealthy donors, argues Ivins resident Andrew Kramer.
Last month, President Trump released his $4.7 trillion 2020 budget, titled "A Budget for a Better America. Promises Kept. Taxpayers First.". Once again, there's a flagrant disconnect between Trump's words and his actions. In fact, the budget reveals Trump's true values and priorities which betray the American people, including his enthusiastic supporters.
For starters, Trump promises to control deficit spending, yet his budget would cause trillion dollar deficits for the next four years, even with its wildly unrealistic projection of 3 percent annual GDP expansion. Then, to add to our pain as taxpayers, Trump calls for the massive tax cuts for the wealthy elite enacted by the GOP in 2017 (that added $1.9 trillion to the deficit) to be made permanent. This, when its obvious we need to increase tax revenues by requiring the corporate and wealthy elite to pay a larger and fair share.
Regarding healthcare, Trump's budget calls for slashing Social Security disability insurance, reversing Medicaid expansion and ending the Affordable Care Act. Although Trump recently reversed position on terminating the ACA due to bipartisan pressure, Trump's intention to deny health insurance for millions and undermine Medicaid is clear. He would cause and remain oblivious to the suffering of millions.
Funding for public education would be cut 10 percent, the third consecutive year of cuts, while funds and tax breaks would be given to private schools. After-school programs for students of low-income families would be eliminated. In addition to weakening public education, Trump would make college less affordable by cutting funding for several key programs that assist with college tuition. These measures would exacerbate the student loan debt which already totals an astounding $1.5 trillion.
Funding for the working poor would be slashed. Food stamps, which assist some 4 million children who live in poverty, would be cut 30 percent over the next ten years. Subsidies for public housing would be reduced making it more difficult for low-income people to find housing. Home energy assistance would be eliminated, causing many to suffer in the cold during winter.
The budget for the Labor Department would be cut, thereby reducing funding for unemployment insurance, even though unemployment is expected to increase as the economy slows. The job corps that helps poor young people enter the workforce would be slashed. Federal employees — those thousands who struggled during Trump's government shutdown - would be hammered once again with less take-home pay and pension cuts.
And, as expected from one of the most anti-environment presidents in our history, funds for the Environmental Protection Agency would be slashed almost one-third next year alone. This underfunding combined with a former coal industry lobbyist now heading the EPA, would virtually assure us of more polluted air and water, more toxins in our food chain, and other hazards that threaten our health and the planet.
Then, in spite of Trump claiming "we must write the next chapter of the great American adventure, turbocharging the industries of the future and establishing a new standard of living for the 21st century", his budget does the opposite. Funding for research and development in technology, invention and innovation is slashed. In the first year alone, funds for the National Science Foundation, the National Institutes of Health, and the Centers for Disease Control and Prevention are each cut 12 to 14 percent. Investment in R&D for renewable energy and energy efficiency is virtually eliminated. Subsidies for renewable energy are reduced while unnecessary subsidies for fossil fuels, including coal, are increased. For our decaying infrastructure, Trump allows $200 billion over ten years when trillions are required. Further, his budget calls for cuts to agencies that implement infrastructure upgrades — a 22 percent cut to the Army Corp of Engineers and almost 20 percent reduction to the Department of Transportation.
Trump's budget claims to support working families by assisting families with child-care needs and providing paid parental leave, however, this is deceitful lip service. Appropriations for these programs are either woefully inadequate or non-existent.
In summary, while statements in Trump's budget profess to put the needs of the American worker first, in reality, his budget betrays the American people. While the rich benefit, working people get the shaft. Public investment essential to our future is deprived. As Robert Greenstein, president of the Center on Budget and Policy Priorities, states: "Altogether, the budget would make poverty deeper and more widespread, increase the ranks of the uninsured, exacerbate inequality and racial disparities, and shrink opportunities for those trying to get ahead." In short, this social and economic decline would not "make America great again".
Fortunately, we have a Democratic majority in the House that will not allow Trump's harmful assault on America to stand. But that does not absolve Trump of his underlying agenda, his values and motivations which are to transfer as much wealth as possible from the vast majority to the wealthy elite.
The question is, how long will his supporters remain blind to his false promises? And how long will congressional Republicans (and corporate Democrats) remain beholden to their wealthy donors instead of the American people?
Andrew Kramer lives in Ivins.


Αthens, Thessaloniki and Arahova welcomed the most Greek visitors in February, according to recent data released by INSETE.
The Tourism Ministry has carried out efforts to safeguard Greek small and medium-sized businesses active in room or apartment rentals.
Authorities of the island of Lefkada are interested in extending the tourism season through the development of sea travel and new theme-oriented products.
Scoot offers travelers from Athens the opportunity to fly to Laos via Singapore, following the launch of services between the two Asian countries.
The "History War Museum Anastasios Liaskos" on Zakynthos, which offers valuable information on Greece's history, has officially opened to the public.
The NJV Athens Plaza hotel was named a "Champion" of sustainability at the 4th Annual Sustainability Summit, which was recently held in the Greek capital.
The "Greek Maritime Golf Event", the top golf tournament for the shipping community, is returning for the fifth year to Costa Navarino during June 8-9.
Crete's Olive Green Hotel is among the 25 best in Greece, according to TripAdvisor's Travelers' Choice Awards for Hotels 2019.
The Arabian Travel Market (ATM) 2019 showcase in Dubai will host its first Hotel Industry Summit as part of its line-up of special events.
This week, GTP Headlines introduces Nikolaos Anthrakidis, the managing director of "Be My Guest Athens", a boutique hotel in the Thissio neighborhood of Athens.


text:
Joel Sherman
The two ways Yankees can make Kyle Schwarber fit their roster: Sherman
Social Links for Joel Sherman
December 3, 2020 9:59am
December 3, 2020 10:11am
More On: new york yankees
These are the Yankees and Mets prospects in Baseball America's Top 100
How a heart attack drastically changed the Yankees' fate in the 1990s
Potential Yankees target hires Scott Boras
Kyle Schwarber fits so naturally — in theory — with the Yankees. They are crying out for lefty bats. The now non-tendered former Cub has a pull swing that is made for 40-plus homers with the short porch in The Bronx. He is probably not going to be expensive, likely favoring a one-year contract to rebuild his value for next year's free agent market.
But then theory smashes into the reality of the Yankee roster.
Giancarlo Stanton is locked in as the DH. Luke Voit, the defending MLB homer champ, is at first base. Schwarber can be put in left. But while the right field at Yankee Stadium would favor his swing, the large swath in left would exacerbate his ordinary (or worse) defense.
Two of the Yankees' problems are having too many lumbering DH types that negatively impact their defense and too many players whose profile is walk, strikeout or homer — and that three true outcome model defines Schwarber.
There is a lot of Gary Sanchez in Schwarber's offensive game. Like Sanchez, there were early hints of an all-around terrific hitter — he was one of now former Cubs president of baseball operations Theo Epstein's favorite players because Epstein sensed the skill to be a complete hitter with power. But over time Schwarber — like Sanchez — has morphed into more of an all-or-nothing pull monster.
Schwarber hit .188 in the abbreviated 2020 season. Only five players who came to bat at least 175 times had a worse average — with Sanchez at the bottom at .147. The Yankees decided to stick with Sanchez and tender him a 2021 contract before Wednesday's deadline despite being in payroll-slashing mode.
Perhaps even more payroll obsessed, the Cubs did not want to invest the $8 million-ish it would take to keep Schwarber. It continued the tear down of their 2016 champion. Jon Lester is a free agent. The Cubs also non-tendered center fielder Albert Almora, like Schwarber, a high first-round pick of the Epstein administration.
But here is something to keep in mind about Sanchez and Schwarber — even in difficult seasons they remained among the majors' best in exit velocity. And the Yankees love them some exit velocity. Schwarber is a year removed from 38 homers. He was the first player the Yankees pursued in the summer of 2016 when they had Aroldis Chapman on the trade market. But back then, Schwarber was off limits and the Yankees landed Gleyber Torres.
Kyle Schwarber finally there for Yankees' taking after Cubs release
There are scouts who believe Schwarber can use the whole field better and become a better overall hitter without sacrificing much power because he has the strength to get the ball out to left-center and center. In this way, there are some similarities to Michael Conforto, picked 10th in 2014 when Schwarber was drafted fourth. Conforto's offensive profile improved in 2020, in particular, when he used his strength throughout the whole field.
There is enough there for Schwarber to entice the Yankees, whose only regular lefty hitter at the moment is switch-hitter Aaron Hicks. How could they make him fit?
They sign him to play left field, use Mike Tauchman or a re-signed Brett Gardner as a late-game defensive substitute and deploy Clint Frazier as a trade piece to help land starting pitching. Opposing executives have told The Post the Yankees have at least tested the waters on what kind of return they could get on players such as Frazier, Tauchman and Miguel Andujar.
The Yankees use Voit as a central trade piece for starting pitching and play Schwarber at first base — believing he can handle that position at least as well as Voit, if not better.
mlb hot stove
Read Next Yankees let go of veteran in slew of moves
Phil Mushnick
NFL broadcasts need more of NBC's brief moment of clarity


Our tesol course spot in the Philippines is found in a metropolis that mixes and ancestral heritage with a contemporary company environment in the course of significant development. Crowned the ?�Queen Town from the South?? Cebu has emerged since the state?�s best vacationer and business area.
Create self confidence, practise your Talking skills and create fluency while you center on Finding out as a result of conversation.
I do know none of Korean stars. But Korean does have the confidence to get proud of them selves, and present the world, And that's why their culture can prosper and enchant Absolutely everyone.


We have no old photos of Allt Glas a' Chùil available currently. You might like browse old photos of these nearby places.
Historic Maps of Allt Glas a' Chùil and the local area.
Browse 1 photo books on Allt Glas a' Chùil and the surrounding area.
Read and share your memories of Allt Glas a' Chùil itself or of a particular photo of Allt Glas a' Chùil.


text:
Bianca Belair Net Worth 2021, Kids, Husband, Biography, Age, Height, Weight, Real Hair, Wiki and Career Details
Written by James in Sports Last Updated June 16, 2021
Real Name/Full Name Bianca Nicole Blair
Nick Name/Celebrated Name: Bianca Belair
Birth Place: Knoxville, Tennessee, United States
Date Of Birth/Birthday: 9 April 1989
Parents Name: Father – Leonard Blair
Mother – Travonda Blair
Siblings: Leonard Jr., Jeffery Phillips, Jermaine, and Jaster
School: Austin-East Magnet High School
College: University of South Carolina
Religion: Unknown
Boyfriend: N/A
Husband/Spouse Name: Kenneth Crawford (Montez Ford) (m. 2018)
Profession: Professional Wrestler
Bianca Belair is a renowned professional wrestler who is currently signed in to WWE. In WWE, she performs under a ring name Bianca Belair on the SmackDown brand. Currently, she is the champion in the WWE Smackdown Women, which is her first reign. They came to be among the first African American to make headlines in WrestleMania together with Sasha Banks.
Before Bianca, Belair was mainly known as the EST of NXT, but currently, he is now the EST of WWE. She gained much of her popularity after earning the All-American and All-SEC honors, which paved the way to her success. Keep reading this article as we share more details regarding the net worth, biography, early life, personal life, and career details regarding Bianca Belair.
Bianca Belair was born on 9th April 1989 in Knoxville, Tennessee, in the United States of America. Her current residence is in Orlando, Florida, USA. Her full name is Bianca Nicole Crawford, and she contains the Afro-American ethnicity. Her father's name is known as Leonard Blair, while her mother is known as Travonda Blair.
Additionally, she also has four siblings known as; Leonard Jr., Jeffery Phillips, Jast, and Jermain. While in her early life, she got involved as an athlete following the passion she had for her career. She claims that the history of her family, starting from her grandfather, inspires her career since her childhood.
Belair attended school in his birthplace area at Knoxville, Tennessee, in Austin-East Magnet High School. While in school, she got involved in several sports in which she had much success in different sporting activities. During her time in school, she was a track and field athlete in which she competed in the hurdles.
In her Higher education life, she attended 3 universities which include the University of South Carolina, Texas A&M University, and later she went to complete her studies at the University of Tennessee. At the University of Tennessee, she got named to the SEC's Academic honor roll for 2011 and 2012. During the time she was in the university, she was a powerlifter and a CrossFit competitor. Following this, she got featured in Crossfit.com, Femme Rouge Magazine, and in RX magazine. Nevertheless, her shifting rib syndrome forced her to abandon her career in Crossfit.
Personal Life: Husband, Kids, Family, Marriage
Bianca Belair got engaged in 2017 to her fellow professional wrestler known Kenneth Crawford. Kenneth competes on SmackDown with the ring name Montez Ford. They later tied the knots on 23rd June 2018. Though they don't have children together, Belair became the stepmother to Montez Ford's two children, who were from the previous relationship he was in.
Is Bianca Belair Lesbian/Gay?
No, Bianca Belair is neither gay nor lesbian. She is of a straight sexual orientation and in her love life together with her husband, Montez Ford. She has had no rumors regarding her being gay or lesbian.
Bianca Belair commenced her career as a CrossFit competitor together with being a powerlifter in her university. She was, however, forced to stop her career following an Intercostal Chondritis that is known for shifting the rib syndrome.
Following the end of her CrossFit career, Bianca joined the WWE prospects through which she gave out her details. She then later received a social media message from Mark Henry, who was a WWE veteran, that informed her that she could receive a trial following her profile on the CrossFit career. After having the trials done, she got a contract signing to WWE on 12th April 2016. Her signing was allocated to the WWE Performance Center located in Orlando, Florida.
She then made her first appearance in WWE on a segment known as NXT live event in June 2016, where she claimed to be the 'EST of NXT…. Prettiest, worst, strongest. She, however, faced a defeat on her first featuring. She was later featured in NXt in September 2016, where she defeated Aliyah.
In the following NXT Women's Championship, she was eliminated by her opponents known as Payton Royce and Billie Kay. She later defeated Aliyah together with Sage Backett in the Me young Classic, though eliminated on the second round by Kairi Sane, who became the winner of the championship.
Belair commenced having success in her career, which saw her have several defeats, including defeating Lacey Evans, Candice leRae, Dakota Kai, Aliyah, and Deonna Purrazzo. This saw her get featured in the WrestleMania Women's Battle Roayl on WrestleMania 34. Nevertheless, she got eliminated by Becky Lynch.
In December 2018, she won against Mia Yim, Lacey Evans, and Io Shirai, which saw her win the Fatal 4-Way match. As a result of this, she became the contender for the NXT Women's Champion. She then lost her undefeated streak of 367 days in January 2019 after losing to Baszler.
Bianca Belair won the Women's Battle Royal in the NXT Women's Championship in January 2020, where she won against Rhea Ripley. She was then featured in the Women's Royal Rumble Match as a Number 2 after the elimination of other legends in wrestling. She, however, was defeated by Charlotte Flair after 33mins and 20secs.
She saved The Street Profits, the team his husband Montez Ford plays for severally. She was drafted to the Smackdown brand in 2020, in which she defeated Natalya and Billie Kay in October 2020. In January 2021, she won the Royal Rumble Match by eliminating Rhea Ripley, making her the second African American superstar after The Rock to win in WWE history. She was in the match for 52 minutes which was a record in the Women's Roayl Rumble match.
Later, she challenged Sasha Banks in WrestleMania 37 hence winning the Smackdown Women's Championship, which was her first title to win in her career.
Bianca Belair has been very passionate about her career. Through her determination in her career, she has seen several successes, which have seen her win several awards. Some of her awards and achievements she has had include the following;
She got racked 9 on the 10 Female Wrestlers of the year 2018
In 2020, she got ranked 23 in PWI Women's 100 on the top 100 female wrestlers
She is the first African-American woman on the headline WrestleMania and to win WWE title.
Net Worth, Salary & Earnings of Bianca Belair in 2021
Bianca Belair has an estimated net worth of $5 Million. This comes from the several wins she has had in her career. In addition to this, she has been featured in several competitions. Her net worth is expected to keep increasing with the expectation of having several wins in the future.
Bianca Belair Social Media Platforms
Bianca Belair has been active on social media platforms from which she has a following of more than 331k in her Twitter account, 785k followers on Instagram and 1.3M on her Facebook account.
You can follow her from the following:
Bianca Belair Twitter Account
Bianca Belair Facebook Account
Bianca Belair Instagram Account
She maintains a long weave which she also uses in attacking her opponents at times.
In conclusion, Bianca Belair has had an amazing story. Though she had invested in a different career line while in her education days, she was later forced to stop the career, which later saw her get into wrestling. Her ladder is increasing, and she is expected to have much more success in her career following the determination and passion she has for her career.


text:
Healthy Hearing About Us
About Healthy Hearing
The mission of Healthy Hearing is to improve the quality of life for people with hearing loss. We do so by connecting consumers to high-quality hearing care providers via our directory of consumer-reviewed clinics located across the U.S. We also strive to provide top-notch content on hearing loss and hearing loss treatments.
Healthy Hearing launched in 2001 as a way to help people find information about hearing loss and hearing aids on the Internet. Since then, we've grown substantially, with over four million site visitors in 2018.
The Healthy Hearing Report has earned Digital Health Awards for the past five years running, including two bronze awards in 2019 for coverage of essential oils and hearing loss and the unrecognized impact of exhaustion from hearing loss, a silver award in 2018 for our article about hearing aid research using spider silk, and in 2017 for a survey we conducted about unexpected hearing hazards.
With 3,600+ clinic profiles and more than 25,000 consumer reviews, our hearing care directory is the largest of its kind in the continental United States.
Be part of the conversation on social media and connect with us on Facebook, Twitter, LinkedIn and Pinterest. See our contact page if you need one-on-one assistance.
When you or a loved one needs hearing healthcare, use our Find a clinic tool to get the help you need.
Mandy Mroz, AuD, president
Dr. Mandy Mroz is the president of Healthy Hearing. As an audiologist, her previous experience includes hearing research, customer service and training. She earned her bachelor's degree from the University of Northern Iowa, her master's degree from The University of Memphis and her doctorate in audiology from the University of Florida. Mandy's dedication to serving people with hearing loss guides her as she oversees the strategy and daily operations of Healthy Hearing, keeping everyone on track. She lives in Red Wing, Minnesota, with her husband, her daughter and their prolific vegetable garden. In addition to gardening, Mandy enjoys cooking, reading, exploring nature and traveling.
Susanne Jones, BC-HIS, customer support manager
Susanne Jones is the customer support manager for Healthy Hearing. She previously oversaw a hearing clinic for a major hearing instrument manufacturer. Susanne earned her bachelor's degree in communication from Bradley University and is a licensed hearing instrument specialist who is Board Certified in Hearing Instrument Sciences. Because she has hearing loss and is a long-time hearing aid user herself, Susanne understands the challenges of hearing impairment and knows the benefits of modern hearing technology firsthand. She loves knowing the work she does every day helps hearing care practitioners all over the country provide quality care to people with hearing loss. Susanne resides in Roscoe, Illinois, with her husband and two children. Outside of the office, she enjoys sports, mid-century modern architecture and design, cooking and visiting wineries.
Jennifer Dahmer, HIS, customer support specialist
Jennifer Dahmer is the customer support specialist for Healthy Hearing. As a licensed Hearing Instrument Specialist, Jennifer knows the impact of helping the hearing impaired. She earned her bachelor's degree in journalism from the University of Wisconsin - Madison and has spent her career in healthcare sales and marketing. At Healthy Hearing, she prides herself on delivering excellent support to providers and patients alike, diligently working to connect people with hearing loss to professionals dedicated to improving lives through hearing healthcare. Jennifer lives with her husband and three children outside of Milwaukee, Wisconsin, and enjoys homesteading hobbies including raising goats, chickens, ducks and dogs.
Joy Victory, managing editor
Joy Victory is the managing editor of Healthy Hearing. She has extensive experience editing consumer health information. Her training in particular has focused on how to best communicate evidence-based medical guidelines and clinical trial results to the public. Joy earned her bachelor's degree in journalism and mass communication from New Mexico State University. She lives in Austin, Texas, with her husband, daughter and cat. She enjoys spending time outdoors, exploring Austin's live music scene, and shelling whenever she is lucky enough to be at the beach.
Stephanie Meyers, SEO specialist
Stephanie Meyers is the search engine optimization (SEO) specialist for Healthy Hearing. Her work makes it easy for consumers searching for hearing help online to find Healthy Hearing. Throughout her marketing career, Stephanie has worked with agencies who have served clients in many different industries including the hospitality and healthcare businesses. As part of the Healthy Hearing team, she is proud to work for a company that helps educate people and improves their lives every day. Stephanie lives in Portland, Connecticut, with her husband and two cats. In her spare time, she enjoys cooking and traveling anywhere she can.
Becky Lemons, senior developer
Becky Lemons is a senior developer for Healthy Hearing. She earned her master's degree in medical engineering from The University of Washington and has over 15 years of experience in web and software development in the medical device and healthcare industries. At Healthy Hearing, Becky is responsible for all phases of design, development, testing and database management. She feels fortunate to be able to use her skills to help people with hearing loss receive quality care. Becky lives in Wenatchee, Washington, with her husband and two daughters. She enjoys camping, boating, snowboarding and playing fantasy football.
Bill Talkington, PhD, junior developer
Dr. Bill Talkington is a junior developer for Healthy Hearing. He earned his PhD in neuroscience from West Virginia University, where he studied vocalization processing in the human auditory system with functional MRI and EEG. After transitioning from academic research into a career in web development, he feels fortunate to work for a company that not only combines these two interests but one that aims to educate and help people achieve their best hearing health. Bill lives in Uniontown, Pennsylvania, with his wife and two daughters. In his spare time, he enjoys playing guitar, woodworking, biking, gardening and volunteering in his community.
Brian Gordon, front-end developer
Brian Gordon is the front-end developer for Healthy Hearing. He earned a bachelor's degree in sociology from California State University-San Bernardino. For the past several years, he has worked with digital marketing companies to build web pages and campaigns for dozens of clients across multiple fields. Brian's focus for Healthy Hearing is on building and improving user experiences to help connect people with clinicians as easily as possible. Brian lives in Glendora, California, though he likes to travel far away from home as much as possible. He spends his free time swimming, body surfing, watching terrible movies and learning how to build his own video games.
All new content published on Healthy Hearing is proofread, fact-checked and edited before publication. We use only trustworthy, medically vetted information and research to provide the latest and most accurate hearing care information. We also continually update older content.
Managing Editor Joy Victory is in charge of editorial production for the site. She has extensive experience in healthcare journalism and online content management spanning two decades. Read more about Joy.
Debbie Clason, features writer
Freelance features writer Debbie Clason has been penning articles for Healthy Hearing since 2013, covering a broad range of topics related to hearing loss, hearing care and hearing aids. Debbie has authored some of our most perennially popular pieces, including airplanes and ear pain, the best apps for learning sign language, and even earwax. She has more than 40 years experience as a journalist, corporate marketing director and freelance writer and owns the marketing and communication business Clason Communcations. Debbie received her bachelor's degree in journalism and master's degree in education from Indiana University. The (very proud) mother of three grown children lives in Fountain Hills, Arizona. Her hobbies include hiking, playing classical guitar and camping with family.
Glenn Schweitzer, tinnitus columnist
Columnist Glenn Schweitzer writes our monthly Tackling Tinnitus column, in which he shares his personal insights overcoming struggles with tinnitus and Meniere's disease. A blogger, book author and passionate advocate for people with vestibular disorders, Glenn wrote his first article for us in 2017. Since then, his columns have resonated with many thousands of readers struggling with tinnitus.
Temma Ehrenfeld, medical writer
Freelance writer Temma Ehrenfeld is our newest contributor, joining the team in 2019. A longtime healthcare journalist who also has hearing loss, she has written for publications from the New York Times and Wall Street Journal to Popular Science and Psychology Today. Temma writes from a place of informed empathy. She loves to tackle emerging or medically complex topics in hearing care, such as hearing aids for profound hearing loss, telehealth and hearing care, and how rare disease can impact hearing. She is the author of a children's book series and a novel, and spends free time kayaking, dancing, at the theater and discussing poetry.
About our commitment to you
Better hearing is not a destination, but a process. We hope that our educational resources improve your personal journey and/or the journey of a loved one.
We encourage and welcome your input so we can continue to improve this process and help others. If you have any feedback, please feel free to contact us using our online form or give us a call at (800) 567-1692.
Thanks for visiting and we sincerely wish you Healthy Hearing!


social customer experiences that deliver real business results@BRIANSOLIS1Remember when traditional marketing worked?24Editorial calendars, tech stunts and real-time marketing programs say the opposite of thoughtfulness. 5From Monologue to Dialogue678BrandWhat we say and doExperienceWhat people feel and shareExperience Divide911Your strategy is defined by the questions you ask and the answers you get.12ROIReturn on IgnoranceWhat is brand?14What is experience?1619#LEARNTO UNLEARNBehind every screen, every expression and impression, is a human beingnot a consumer.21WELCOME TO THE #EGOSYSTEMhttp://notable.ca/a-photographer-is-removing-phones-from-his-images-to-show-how-addicted-we-are/2223242526Master Total Re-CXingPeople look at their phones 1,500x each week.They spend 177 minutes on their phone per day.The Future of Experience in 1 Picture293034Micro-moments unfold through a variety of common I want scenarios that help people take steps or make decisions I want to learnI want to buyI want to knowI want to goI want to do90%of smartphone users are not absolutely certain of the specific brand they want to buy when they begin looking for information online.73%say getting useful information from a business is the most important attribute when selecting a brand.404142More Mindfulness, Less Strategy.Marketing as Social ObjectsImpressionDeepEngagementOut of Mind orConnected Experience43THE A.R.T. OF ENGAGEMENTCTIONSAEACTIONSRRANSACTIONST44Remember when you were told to stop daydreaming and how it made you feel? Please dont stop.We need dreamers (and doers) now more than ever.#dontquityourdaydream 46Brian Solis bsolis@prophet.comAltimeter Group, a Prophet Companybriansolis.com@briansolisLinkedin.com/influencer/briansolisFacebook.com/thebriansolis47You cannot move in a new direction if you dont lead the wayYou cant change anything if you change nothing to do so.
First Year Experience WHERE BUSINESS MEETS FASHION.
Education and Economics FASA presentation July 28, 2009 Where Education Meets Experience Where Education Meets Experience.
IPM Asset Solutions: Where Innovation Meets Experience.


Love Your Body Events at Reston Town Center kicks of Virginia Yoga Week.
Instructor from Bikram of Great Falls teaching Bikram to patrons at the event.
Diana Falk, left, of Centreville, said, "It's my first time coming out to the Love Your Body event, I think it's great and a great way to bring people together."
Members of Sterling Yoga, Pittsburgh, Pa. From left, Sirgoh Koaur, Ramdep Kaur and Elkie Derekson.
Despite the 90 degree weather, hundreds of yoga enthusiasts gathered Sunday for the Love Your Body event in Reston Town Center. This annual event was organized by Beloved Yoga, which has studios in Reston and Great Falls.
The event lasted until 4 p.m. and throughout the whole day there were a variety of activities available; free yoga classes, live music, complimentary massages; energy healing demonstrations; and healthy food samples, as well as the opportunity to ask questions about alternative healing therapies.
Michelle Mallo-Esposito, of Herndon, said, "This is a fabulous opportunity to bring like-minded people together to celebrate the art of an alternative cure."
New this year to event was a kids' tent that also offered yoga classes such as story time yoga and awaken the superhero.
Ramdep Kaur, first time to the event, said, "This event is a great way to bring enthusiast of yoga together to share knowledge and experience."
Love Your Body Day is a kick off for Virginia Yoga Week, June 10 –17. In celebration of yoga throughout the Commonwealth, Virginia yoga studios and teachers will be offering free classes along with special workshops and karma yoga events throughout the week.
For more information contact Beloved Yoga at belovedyoga@yahoo.com or call them at 703-860-9642.