text:
Interaction of the growth and tumour suppressor NORE1A with microtubules is not required for its growth-suppressive function
Anna Moshnikova1,
Sergey Kuznetsov1 &
Andrei V Khokhlatchev1
The NORE1 protein was identified in a yeast two-hybrid screen as a Ras effector that binds Ras protein in a GTP-dependent manner. NORE1A is a growth and tumour suppressor that is inactivated in a variety of cancers. In transformed human cells, both full-length NORE1A protein and its effector domain alone (amino acids 191–363) are localized to microtubules and centrosomes. However, the mechanism by which NORE1A associates with these cytoskeletal elements is not known; furthermore, whether centrosomally-associated or microtubule-associated NORE1A suppresses tumour cell growth has not been yet established.
We have shown that purified NORE1A fails to bind to microtubules in vitro suggesting that other protein(s) mediate NORE1A-microtubule association. Using mass-spectrometry, we identified the Microtubule-Associated Protein 1B (MAP1B) and its homologue C19ORF5 as NORE1A interaction partners. Suppression of C19ORF5 expression by RNA interference (RNAi) and immunodepletion of C19ORF5 protein from cell extracts showed that binding of NORE1A to microtubules is not dependent on C19ORF5. Conversely, RNAi suppression of MAP1B revealed that MAP1B is required for association of NORE1A with microtubules. RNAi-mediated depletion of C19ORF5 or MAP1B did not prevent centrosomal localization of NORE1A. Moreover, the depletion of C19ORF5 or MAP1B did not prevent NORE1A's ability to suppress tumour cell growth.
The interaction of NORE1A with microtubules is mediated by MAP1B, but not C19ORF5 protein. Interaction of NORE1A with centrosomes is not dependent on C19ORF5 or MAP1B, and appears to involve a different mechanism independent of binding to microtubules. The NORE1A microtubular localization is not required for growth suppression.
NORE1A interacts with C19ORF5 and MAP1B proteins
We have previously shown that NORE1A associates with microtubules and that this association is most likely indirect since purified NORE1A cannot efficiently bind to microtubules assembled from pure tubulin [1]. To identify proteins that mediate association of NORE1A with microtubules, we expressed FLAG-tagged full-length NORE1A or effector domain (amino acids 191–363) in HEK293 cells and examined NORE1A-bound proteins by mass spectrometry. Among the most abundant NORE1A protein partners, we identified two distinct microtubule-binding proteins: C19ORF5 and MAP1B. The interaction between ectopically expressed NORE1A and endogenous C19ORF5 and MAP1B proteins was confirmed by coimmunoprecipitation (Figs. 1A–B and 1D).
Interaction of C19ORF5 and MAP1B proteins with wild type and mutant NORE1A. A-C: HEK293 cells were transfected with FLAG-tagged wild type NORE1A, fragments of NORE1A, or deletion mutants as indicated. Cell lysates were precipitated for FLAG and the precipitate was probed with antibody against C19ORF5 and MAP1B (upper panels). Lower panels show expression of FLAG-tagged proteins in cell extracts. Asterisk denotes NORE1A 1–268 degradation product. D: A549 cells were infected with retrovirus coding for hrGFP-tagged wild type NORE1A or hrGFP alone as indicated. Cell extracts were precipitated for NORE1A and the precipitate was probed with antibody against MAP1B (upper panel). Lower panels show expression of NORE1A and MAP1B in cell extracts.
C19ORF5 interacts with NORE1A but does not mediate its binding to microtubules
Both full-length NORE1A and its effector domain alone are capable of interacting with endogenous C19ORF5 protein in HEK293 cells (Fig. 1A). The N-terminal portion (amino acids 1–268) and the C-terminal portion (amino acids 250–416) of NORE1A failed to interact with C19ORF5 (Fig. 1A), suggesting that the intact effector domain, amino acids 191–363, mediates NORE1A-C19ORF5 interaction.
To map the NORE1A-C19ORF5 interaction interface more precisely, we made several small deletions in the NORE1A effector domain and tested these proteins for interaction with C19ORF5. Figure 1C shows that the deletion of amino acids 227–253 completely abolished NORE1A-C19ORF5 interaction. The smaller deletion mutant, NORE1A Δ236–253, also failed to interact with C19ORF5; however, the deletion mutant NORE1A Δ246–253 retained binding activity. Thus, the region 227–246 within the NORE1A effector domain is responsible for interaction with the C19ORF5 protein.
To determine whether C19ORF5 protein is required for the interaction of NORE1A with microtubules, we first depleted endogenous C19ORF5 protein using RNAi and examined the interaction of NORE1A with microtubules in an in vitro cosedimentation assay. Despite significant RNAi-mediated depletion of the endogenous C19ORF5 protein from HEK293 cells expressing NORE1A, the ability of NORE1A to interact with microtubules was not diminished (Fig. 2A). To completely remove C19ORF5, we immunodepleted the HEK293 cell extract with anti-C19ORF5 antibody. Purified NORE1A was then added to immunodepleted or mock-depleted cell extract and the ability of NORE1A to interact with microtubules was examined. Figure 2B shows that, despite efficient C19ORF5 immunodepletion (upper panel), the depleted cell extract was as capable of mediating NORE1A interaction with microtubules as mock-depleted extract (middle panel, lanes 1–4). In addition, purified C19ORF5 protein could not induce interaction of purified NORE1A with microtubules (Figure 2B, middle panel, lanes 5 and 6), although the purified C19ORF5 itself interacted strongly with microtubules (data not shown). The depletion of C19ORF5 protein using RNA interference in A549 cells expressing GFP-NORE1A also did not diminish the ability of GFP-NORE1A to interact with microtubules in vitro (Fig. 2C).
The C19ORF5 protein does not mediate NORE1A binding to microtubules in vitro. A: C19ORF5 protein was depleted by RNA interference in HEK293 cells expressing HA-tagged NORE1A. Equal amounts of cell extracts were probed for C19ORF5 (upper panel) or used to examine the ability of NORE1A to bind to microtubules using the microtubule cosedimentation in vitro assay. B: HEK293 cell extract was immunodepleted of the C19ORF5 protein by incubation with 4G1 antibody followed by Protein A/G plus agarose, or mock-depleted. Equal amounts of C19ORF5-depleted and mock-depleted extracts were probed for C19ORF5 (upper panel). The ability of purified FLAG-NORE1A to interact with microtubules after preincubation with C19ORF5-immunodepleted extract (lanes 1–2), mock-immunodepleted extract (lanes 3–4), or purified FLAG-C19ORF5 (lanes 5–6) was examined as described in the Methods. C. C19ORF5 protein was depleted by RNA interference in A549 cells expressing GFP-tagged NORE1A. Equal amounts of cell extracts were probed for C19ORF5 (upper panel) or used to examine the ability of NORE1A to bind to microtubules as described in Figure 1 (two middle panels). Lower panel, the ability of GFP moiety, expressed alone in A549 cells, to bind to microtubules was examined.
We have shown previously that full-length NORE1A or its effector domain, tagged with N terminal GFP, localizes to microtubules and centrosomes in living A549 cells [1]. We used this cell system combined with RNAi to examine the role of C19ORF5 in NORE1A intracellular distribution. Figure 3A upper panel shows that the C19ORF5 protein was efficiently depleted from A549 cells; importantly, this depletion has no effect on the GFP-NORE1A abundance (Fig. 3A, lower panel). However, the GFP-NORE1A associated with microtubules (visualized by α-tubulin staining) as efficiently in C19ORF5-depleted cells as in control siRNA-depleted cells (Figure 3B). The colocalization was observed in both interphase (Fig. 3B, middle row) and mitotic (Fig. 3B, bottom row) cells. These results indicate that C19ORF5 is not required for targeting of NORE1A to microtubules in vivo.
The C19ORF5 protein does not mediate NORE1A binding to microtubules in living cells. A, B: A549 cells expressing GFP-NORE1A were transfected with anti-C19ORF5 siRNA pool or control siRNA as described in the Methods. An aliquot of cells was used to examine efficiency of C19ORF5 depletion (A, upper panel) and NORE1A expression level (A, lower panel) while other cells growing on coverslip were fixed, processed for immunofluorescence and imaged as described in Methods (B). In B, each panel shows NORE1A, microtubules (stained with α-tubulin), centrosomes (stained with pericentrin) and a superimposed image. Arrows indicate centrosomes. Bar, 10 μm.
MAP1B interacts with NORE1A and is required for its binding to microtubules
MAP1B is a large (ca. 2500 amino acids) microtubule-associated protein consisting of several domains. Figures 1B and 1D show that NORE1A and the effector domain can interact with endogenous MAP1B protein in HEK293 and A549 cells, respectively. Furthermore, interaction required the intact NORE1A effector domain (amino acids 191–363) since neither the N-terminal portion (aa 1–268) or the C-terminal portion (aa 250–416) of NORE1A could interact with MAP1B (Fig. 1B).
To determine whether MAP1B protein mediates the interaction of NORE1A with microtubules, we depleted endogenous MAP1B from A549 cells by RNAi and examined the ability of NORE1A or its effector domain to bind to microtubules in vitro. Figure 4A shows that endogenous MAP1B expression was virtually eliminated by RNAi in A549 cells and Figure 4B shows that NORE1A was unable to interact with microtubules in the absence of the MAP1B protein.
The MAP1B protein is required for NORE1A binding to microtubules. A, B: A549 cells were co-transfected with RNA oligos labelled with the Cy3 dye and anti-MAP1B siRNAs or control siRNA. After one day cells were infected with retrovirus encoding GFP-NORE1A. Two days later, cells were sorted using GFP and Cy3 markers, allowed to recover overnight, lysed and equal amounts of cell extract were probed for expression of MAP1B protein (A, upper panel) and GFP-NORE1A expression level (A, lower panel). Lysates of GFP and Cy3- positive cells were examined for the ability of GFP-NORE1A to interact with microtubules as described in Figure 1 (B). C: A549 cells expressing GFP-NORE1A were transfected with anti-MAP1B siRNA pool or control siRNA. Cells were fixed, processed for immunofluorescence, and imaged as described in Methods. Each panel shows NORE1A, microtubules (stained with α-tubulin), centrosomes (stained with pericentrin) and a superimposed image. Arrows indicate centrosomes. Bar, 10 μm.
To determine whether MAP1B protein is required for targeting of NORE1A to microtubules in living cells, we examined the distribution of GFP-tagged NORE1A or its effector domain in A549 cells in which MAP1B has been depleted by RNAi. Depletion of MAP1B resulted in loss of microtubule localization of full-length NORE1A (Figure 4C) and its effector domain, aa 191–363 (See additional file 1: Supplemental Figure 2). Probing of A549 cell lysates where MAP1B has been depleted for NORE1A indicated that the NORE1A level was not changed, ruling out the effect of MAP1B on NORE1A stability (Fig. 4A, lower panel). Thus, our results indicate that the MAP1B protein is essential for interaction of NORE1A with microtubules in vitro and in vivo.
The reason why binding of NORE1A to microtubules is mediated by MAP1B, but not by C19ORF5 is not clear at the present time. For example, it is possible that the C19ORF5 protein mediates NORE1A interaction with microtubules under specific conditions or in specific cell types not tested here.
The effect of MAP1B or C19ORF5 on centrosomal localization of NORE1A
We showed previously that NORE1A is localized to centrosomes in addition to microtubules [1]. This localization was observed for endogenous NORE1A, and for ectopically expressed NORE1A and its effector domain. Song and co-workers showed that the C19ORF5 protein was present on centrosomes in interphase and mitotic HeLa cells [2], while a recent report from the Latif group described centrosomal localization for C19ORF5 only in mitotic cells [3]. The MAP1B protein was also detected on centrosomes in interphase and mitotic cells [4, 5]. We tested whether C19ORF5 or MAP1B play a role in the centrosomal localization of NORE1A.
In A549 cells into which NORE1A was introduced by retroviral-mediated gene transfer, depletion of the C19ORF5 protein did not result in dissociation of NORE1A from centrosomes in interphase (Fig. 3B, middle row) or mitotic (Fig. 3B, bottom row) cells. Depletion of MAP1B protein induced dissociation of full-length NORE1A (Fig. 4C, arrows) and its effector domain (See additional file 1: Supplemental Figure 3) from microtubules, but not from centrosomes. Thus, centrosomal localization of NORE1A is independent of its microtubular localization and does not require C19ORF5 or MAP1B proteins.
In contrast to NORE1A, the centrosomal localization of the closely related tumour suppressor RASSF1A was found to be dependent on the C19ORF5 protein [2], possibly reflecting differences in the regulation or mechanisms of action of NORE1A and RASSF1A. Alternatively, this difference could be caused by the use of different cell systems: HeLa cells in ref. [2], compared with A549 cells in this study.
MAP1B or C19ORF5 are not required for NORE1A to suppress tumour cell growth
In an earlier study, we showed that the NORE1A mutant that was incapable to localize to both centrosomes and microtubules was deficient in growth suppression [1]. However, the issue whether microtubular localization or centrosomal localization of NORE1A is required for suppression of tumour cell growth remained unresolved. Here, we took advantage of the finding that full-length NORE1A and its effector domain dissociate from microtubules but not from centrosomes upon the MAP1B depletion. In A549 cells into which NORE1A effector domain was introduced by retroviral-mediated gene transfer, depletion of the MAP1B protein did not interfere with NORE1A's ability to suppress growth (Fig. 5A). Likewise, the depletion of the C19ORF5 protein (Fig. 5B) and the combined depletion of MAP1B and C19ORF5 (data not shown) have no effect on NORE1A's induced growth suppression.
C19ORF5 or MAP1B depletion does not interfere with NORE1A-induced growth suppression. A549 cells were transfected with anti-MAP1B siRNA pool (A) or control siRNA, or anti-C19ORF5 siRNA pool or control siRNA (B) then infected with retrovirus expressing GFP or GFP-NORE1A effector domain (aa 191–363). Two days later, cells were fixed, processed for flow cytometry and analyzed as described in Methods. The percentage of GFP-positive cells in each phase of the cell cycle +/- mean is shown.
Our previous work identified endogenous NORE1A as centrosomal protein in nontransformed human lung cells and as a centrosomal and microtubular protein in transformed lung cells [1]. However, this study shows that the microtubular NORE1A association is not required for its growth-suppressive activities. This raise the possibility that it is centrosomal NORE1A that is important for growth suppression. Further studies are required to determine the mechanism of NORE1A centrosomal localization and the importance of its targeting to centrosomes for suppression of tumour cell growth.
Pools of small-interfering RNA duplexes, cat.# L-016881-00 against C19ORF5 and cat.# L-010348-00 against MAP1B were from Dharmacon, Inc. (Lafayette, CO). The Alexa Fluor 488-labelled AllStars Negative control siRNA, cat # 1027284, were from Qiagen (Valencia, CA). RNA duplex labelled with CY3 dye, cat # 4621, were from Ambion (Austin, TX). The anti-C19ORF5 4G1 antibody were from A&G Pharmaceutical, Inc. (Columbia, MD). The monoclonal anti-NORE1A antibody 10F10 was described in ref [1]. Anti-pericentrin antibody, cat.# ab-4448, were from Abcam (Cambridge, MA), the anti-α-tubulin DM-1-A and anti-γ-tubulin GTU-88 antibodies were from Sigma (St Louis, MO), and the anti-MAP1B antibody, cat # 612679 was from BD Biosciences. Secondary antibody conjugates were from Jackson ImmunoResearch (West Grove, PA). All other reagents were previously described by Moshnikova et al. [1].
Plasmids encoding NORE1A were described previously [1]. The human C19ORF5 protein (IMAGE clone; ID 5925583) was purchased from ATCC (Manassas, VA) and subcloned into pCMV5 vector with the N-terminal FLAG tag. This and all other constructs were prepared using standard molecular biology techniques [6] and verified by sequencing.
Cell Lines, Transfection and Retrovirus Production
Cultivation of A549 human lung adenocarcinoma and HEK293 human embryonic kidney cells (ATCC), HEK293 cells transfection and retroviral supernatants production was described previously [1, 7]. The siRNA duplexes were transfected using DharmaFECT1 reagent (Dharmacon) according to the manufacturer's instructions.
Immunodepletion, Immunoprecipitation and Western Blot Analysis
HEK293 cells were lysed in buffer A (30 mM HEPES, pH 7.4, 20 mM KCl, 1mM NaVO4, 20mM NaF, 20 mM β-glycerophosphate, 7.5 mM MgCl2, 1% Triton X-100, 2 mM EGTA, 0.1% 2-mercaptoethanol and protease inhibitors) and the C19ORF5 protein was immunodepleted by overnight incubation with 4G1 antibody adsorbed on Protein A/G+ beads. The beads were pelleted and the supernatant was subjected to a second immunodepletion as described above. For mock immunodepletion, normal mouse IgG was used instead of anti-C19ORF5 antibody. Other procedures were performed as described previously [1, 7].
Immunofluorescent Microscopy and cell preparation
were performed as described previously [1, 7]. In some cases, cells were transfected with siRNA duplexes prior to plating on coverslips. Forty-eight hours after plating, cells were fixed in 100% methanol at -20°C for 5 minutes and processed for immunostaining as described [1]. Digital images were taken under UV illumination with appropriate filter sets at 100× magnification using a LEICA DMIRE2 microscope and captured using IPLab 3.6.5a software (Scanalytics, Rockville, MD). To allow comparison of image intensity within each experiment, images for GFP-tagged NORE1A and α-tubulin were taken at the same exposure and processed identically in the Adobe Photoshop program.
Tubulin-Binding (microtubule cosedimentation in vitro) Assays
Microtubule cosedimentation assay was done with the Microtubule Binding Protein Spin Down Assay Kit (Cytoskeleton Inc. Denver, CO, cat.# BK029), according to the manufacturer's instructions. After assay, the pellet was adjusted to the same volume as the supernatant with GTB. Both supernatant and pellet were adjusted to 1 × SDS gel loading buffer with 4× stock solution. Equal volumes of supernatant and pellet were separated by SDS PAGE and analyzed by western blotting. In some experiments, purified FLAG-NORE1A was preincubated for 1 hour at 37°C with C19ORF5-immunodepleted or mock-immunodepleted HEK293 cell extract, or purified FLAG-C19ORF5 prior to incubation with microtubules.
Cell Cycle Distribution Assay
A549 cells were transfected with anti-MAP1B siRNA pool, or anti-C19ORF5 siRNA pool or control siRNA as descried above. After one day cells were replated in triplicate on 6-well plates and 8 hours after were infected with retrovirus expressing GFP or GFP-NORE1A effector domain (aa 191–363). Two days later, cells were fixed with 1% formaldehyde in PBS, stained with 0.05 mg/ml propidium iodide and analyzed by FACSvantage (Becton-Dickinson, San Jose, CA). Cell cycle distributions of GFP-positive cells were determined by ModFIT software.
The abbreviations are:
NORE1, novel Ras effector 1
RASSF1A:
Ras-association domain family 1 isoform A
HA:
hemagglutinin
peptide tag DYKDDDDK
hrGFP:
Renilla reniformis Green Fluorescent Protein
DAPI:
4', 6-diamidino-2-phenylindole
GTB:
general tubulin buffer
BSA:
kDa:
kilodalton
microtubule-associated proteins.
Moshnikova A, Frye J, Shay JW, Minna JD, Khokhlatchev AV: The growth and tumor suppressor NORE1A is a cytoskeletal protein that suppresses growth by inhibition of the ERK pathway. J Biol Chem. 2006, 281: 8143-8152. 10.1074/jbc.M511837200.
Song MS, Chang JS, Song SJ, Yang TH, Lee H, Lim DS: The centrosomal protein RAS association domain family protein 1A (RASSF1A)-binding protein 1 regulates mitotic progression by recruiting RASSF1A to spindle poles. J Biol Chem. 2005, 280: 3920-3927. 10.1074/jbc.M409115200.
Dallol A, Cooper WN, Al-Mulla F, Agathanggelou A, Maher ER, Latif F: Depletion of the Ras association domain family 1, isoform A-associated novel microtubule-associated protein, C19ORF5/MAP1S, causes mitotic abnormalities. Cancer Res. 2007, 67: 492-500. 10.1158/0008-5472.CAN-06-3604.
Dominguez JE, Buendia B, Lopez-Otin C, Antony C, Karsenti E, Avila J: A protein related to brain microtubule-associated protein MAP1B is a component of the mammalian centrosome. J Cell Sci. 1994, 107 ( Pt 2): 601-611.
Vandre DD, Feng Y, Ding M: Cell cycle-dependent phosphorylation of centrosomes: localization of phosphopeptide specific antibodies to the centrosome. Microsc Res Tech. 2000, 49: 458-466. 10.1002/(SICI)1097-0029(20000601)49:5<458::AID-JEMT8>3.0.CO;2-#.
Sambrook J and Russell, D.W.: Molecular Cloning: A Laboratory Manual. 2001, Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, Third Edition
Khokhlatchev A, Rabizadeh S, Xavier R, Nedwidek M, Chen T, Zhang XF, Seed B, Avruch J: Identification of a novel Ras-regulated proapoptotic pathway. Curr Biol. 2002, 12: 253-265. 10.1016/S0960-9822(02)00683-8.
We thank Dr. Friedrich Propst (University of Vienna, Austria) for providing MAP1B plasmids and Charles Lyons for assistance with Mass Spectrometry. This work was supported by University of Virginia Cancer Centre Grant GF10574 and by a grant from the Wendy Will Case Cancer Fund to A.V.K.
Department of Pathology, University of Virginia, PO Box 800904, Charlottesville, VA, 22908-0904, USA
Anna Moshnikova
, Sergey Kuznetsov
& Andrei V Khokhlatchev
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Search for Sergey Kuznetsov in:
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Correspondence to Andrei V Khokhlatchev.
AM helped conceive of the study, carried out the experimental work, analyzed the data and participated in drafting the manuscript. SK helped conceive of the study and analyzed the data, carried out some of the experimental work, and participated in drafting the manuscript. AK conceived of the study, participated in the design of the experiments, carried out some of the experimental work, analyzed the data, wrote the first draft of the manuscript and edited it. All the authors have read and approved the final version of the manuscript.
Additional file 1: Supplemental Figure 1: Distribution of tubulin in the supernatant and pellet fractions of the microtubular cosedimentation assay was examined. Supplemental Figure 2: Microtubular distribution of GFP-NORE1A191-363 was examined in A549 cells in which MAP1B proteins were depleted by RNA interference. Supplemental Figure 3: Centrosomal distribution of GFP-NORE1A191-363 was examined in A549 cells in which MAP1B proteins were depleted by RNA interference. (PDF 5 MB)
A549 Cell
HEK293 Cell
Effector Domain
C19ORF5 Expression
C19ORF5 Protein


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text:
Q: "Not only… but also" at the beginning of a sentence Do we always have to use inversion when we start a sentence with "not only… but also"? For example:
Is it correct to write/say:

Not only they were tall, but also they were strong.

Or

Not only were they tall, but they were also strong.

Which one is correct?

A: Only this one is correct:

Not only were they tall, but they were also strong.

I think, we have to do inversion every single time a sentence or clause begins with not only. There is no exception to this rule. I think of this type of structure as a pattern that never breaks and one you simply should commit to memory.
One more example:

Not only do I have to now install the operating system again, but also buy a new computer.

It seems to me that we typically do inversion in English when a sentence or clause begins with a negative word or phrase (the operative word here is begins). One of the most common words of that group that immediately springs to mind is rarely.

Rarely do you see people using desktop computers nowadays. Most people prefer laptops to them because of their better portability characteristics.

However, there is going to be no inversion when not only, or rarely for that matter, is found between the subject and the verb:

I not only have to now install the operating system again, but also buy a new computer.

A: The inversion does not normally carry over to subsequent independent clauses, so

Not only were they tall, but they were also strong

is the correct version.
Subject-auxiliary inversion occurs in declarative clauses only when certain types of element are put in front position. Negatives are one very obvious type of element that trigger subject-auxiliary inversion when fronted:
Never had I seen such chaos.
At no stage were they in danger.
"Not only" is clearly negative, but even when used alone, "only" has the same effect:
Only when I counted the money did I realise the error.
"Only" is not negative, but it is semantically close to a negative, in that only John liked it, for example, entails No one other than John liked it.

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Elton Brand's first trade deadline is over! We discuss how Brand did, Markelle Fultz's run as a Philadelphia 76er is over, as he was traded to the Orlando Magic for a first round pick, a second round pick, and Jonathon Simmons. We discuss the trade, the rollercoaster ride of Fultz in Philadelphia, as well as the James Ennis trade and how the rest of the Eastern Conference improved.


During the raid police detained 5 suspected members of the gang and recovered one hand gun and $250,751.00 in cash denominations of Guatemalan Quetzals, Honduran Lempiras, and US Dollars.
Additionally, Police recovered 50 archaeological pieces, in which there are vases, statues, carved stones shaped head, among others.
The Ministry of Interior stated on their Twitter account that they had notified the Ministry of Culture and Sports about that evidence relevant to the artifacts to determine if the artifacts are legitimate or counterfeit goods.


text:
Seine Tochter ist der Peter ist ein österreichischer Spielfilm aus dem Jahre 1955 von Gustav Fröhlich mit Sabine Eggerth in der Titelrolle. Weitere Hauptrollen spielen Wolf Albach-Retty und Josef Meinrad. Es handelt sich hierbei um eine Verfilmung des Romans "Und seine Tochter ist der Peter" von Edith Zellweker.

Handlung
Der Ingenieur Max Klaar ist geschieden und hat eine halbwüchsige Tochter namens Elisabeth. Weil sie ein richtiger "Wildfang" ist und auch sonst eher Verhaltensweisen von Jungs ihres Alters an den Tag legt, nennt man sie meist "Peter". Bei einer ihrer waghalsigen Unternehmungen mit Freund Peperl fällt sie eines Tages ins Wasser und ertrinkt beinah. Peter hat Glück, dass zufällig die junge Helga in der Nähe ist, die ihr im letzten Moment das Leben rettet.

Peter ist begeistert von Helga, von der sie sich wünschte, dass sie eines Tages vielleicht sogar ihre neue Mutter werden würde. Doch da taucht plötzlich ihre leibliche Mutter, die glamouröse Schauspielerin Nora Christian, auf. Sie nimmt ihre Tochter ohne das Wissen ihres Vaters mit sich und will sie in ihre Künstlerkreise in Wien einführen. Peter fühlt sich jedoch dort nicht wohl und nimmt, von Heimweh getrieben, Reißaus, um zu ihrem Vater und ihren Freunden heimzukehren. Max Klaar, Peperl und Helga heißen sie daheim herzlich willkommen.

Produktionsnotizen
Seine Tochter ist der Peter entstand in Wien-Schönbrunn (Atelier) sowie am Millstätter- und Faakersee in Kärnten (Außenaufnahmen). Die Uraufführung erfolgte am 2. September 1955 in Wien, die deutsche Premiere war am 28. Oktober desselben Jahres in Pforzheim.

Co-Produzent Alfred Lehr übernahm auch die Herstellungsleitung, Co-Produzent Ernest Müller die Produktionsleitung. Fritz Jüptner-Jonstorff gestaltete die Filmbauten, Gerda Walter die Kostüme. Dia Lucca war Choreografin, Wolfgang Glück Regieassistent, Anton Pucher Kameraassistent. Gretl Schörg war auch als Sängerin zu hören.

Derselbe Stoff wurde von Heinz Helbig bereits 1936 erstmals in Österreich verfilmt.

Kritiken
Im Lexikon des Internationalen Films heißt es: "Lebensferne, sentimentale Unterhaltung."

Einzelnachweise

Weblinks

Österreichischer Film
Filmtitel 1955
Literaturverfilmung
Schwarzweißfilm

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This easy No-Churn Strawberry Vegan Ice Cream is rich and creamy. Just blend all ingredients in the blender, pour into a loaf pan and let the magic happen. In a few hours, you have a creamy luscious ice cream that resembles a classic store bought version.
If you haven't noticed, this is the first ice cream recipe on Nurture My Gut. Yup, it's true! I have been working on vegan ice cream recipes for the last few years and unfortunately, all I get is something I am not proud to share.
The flavors are amazing but the texture is icy. Tis the reason, no yummy ice cream recipes, until now! I have finally discovered the secret to creamy ice cream!
Okay, it's not really a secret! Just check out the ingredients in store bought vegan ice creams and you will find xanthan gum in the list. I was actually avoiding adding xanthan gum to my ice cream because I wanted to keep the ingredients clean and minimal, but with so many failures, I decided to give it a chance. I am so glad that I did! The texture is just like an ice cream full of dairy. Seriously, this is so creamy! I am looking forward to making more No-Churn Vegan Ice Cream Recipes for you.
Adding xanthan gum (corn free version)is truly the secret to an extra creamy No-Churn Vegan Ice Cream. You do not even need an ice cream maker, just blend your ingredients, pour into a container and freeze for 5-6 hours. Let your ice cream thaw for a few minutes and then its ready to scoop into bowls or onto my gluten free waffle cones.
I will be working on a vegan chocolate ice cream. Any flavors you want to see on the blog? Please make your request in the comments below!
Add to a loaf pan and cover with plastic wrap. Freeze for 4-5 hours for a soft serve ice cream and 5-6 hours for a "firm" ice cream or overnight. Allow to thaw for 5 minutes before serving.
I have mine in the ice cream maker at the moment. Not sure if I like the almond flavoring though. It tasted really strong before I put it in the maker. I will probably omit it next time. Other than that I think it tastes really good.
I partially watched your video about tortillas. I closed it after the 6th commercial halfway through. I hate waiting on the commercials. I guess Netflix and Hulu have spoiled me.
The ice cream turned out really good except I still didn't like the flavor of the almond flavoring. I will leave it out next time. Just a little too strong for my taste. I may try adding a small amount of strawberry flavoring.
The ice cream had a nice consistency. I poured it into muffin tins and froze it for individual portions. Worked out great!
I have had the same ice issues in my ice cream so I will try the xanthan gum. Have you tried this recipe in an ice cream machine?


GreenKiwi is an education for sustainability programme for New Zealand schools.
We provide outstanding, practical resources to help teachers and students discover sustainability. Our material facilitates effective, student-centred instruction that is assessment friendly, curriculum based, multi-disciplinary, inter-disciplinary and very importantly, fun.
GreenKiwi resources are free for any New Zealand teacher who registers to use them.
Spaceship Earth is our exciting first course that teaches existing curriculum within an education for sustainability framework. Key sustainability concepts are presented in an interpretive context that will engage and fascinate students.
In 2019 GreenKiwi will launch three new courses: 'More good with less stuff' for year 3 and 4 students. 'Sustainability at Home' for year 5 and 6 students. 'Sustainable Development' for year 9 and 10 students. Contact us to be notified when courses launch.
We will be running a fundraising campaign in 2019 for the development of the new courses. We are a not-for-profit Charitable Trust relying on the generosity of people to enable this important work. Sign up to be notified of the campaign launch.


text:
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">

<META http-equiv="Content-Type" content="text/html; charset=UTF-8">
<TITLE>
Uses of Interface org.apache.http.cookie.CookieSpec (HttpComponents Client 4.1.1 API)
</TITLE>

<LINK REL ="stylesheet" TYPE="text/css" HREF="../../../../../stylesheet.css" TITLE="Style">

      Overview
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Uses of Interface
org.apache.http.cookie.CookieSpec

Packages that use CookieSpec

org.apache.http.cookie
The API for client-side state management via cookies.

org.apache.http.impl.cookie
Default implementations for interfaces in
org.apache.http.cookie.

Uses of CookieSpec in org.apache.http.cookie

Methods in org.apache.http.cookie that return CookieSpec

 CookieSpec
CookieSpecRegistry.getCookieSpec(String name)

          Gets the cookie specification with the given name.

 CookieSpec
CookieSpecRegistry.getCookieSpec(String name,
              HttpParams params)

          Gets the cookie specification with the given ID.

 CookieSpec
CookieSpecFactory.newInstance(HttpParams params)

          Creates an instance of CookieSpec using given HTTP parameters.

Uses of CookieSpec in org.apache.http.impl.cookie

Classes in org.apache.http.impl.cookie that implement CookieSpec

 class
AbstractCookieSpec

          Abstract cookie specification which can delegate the job of parsing,
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BestMatchSpec

          'Meta' cookie specification that picks up a cookie policy based on
 the format of cookies sent with the HTTP response.

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BrowserCompatSpec

          Cookie specification that strives to closely mimic (mis)behavior of
 common web browser applications such as Microsoft Internet Explorer
 and Mozilla FireFox.

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CookieSpecBase

          Cookie management functions shared by all specification.

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IgnoreSpec

          CookieSpec that ignores all cookies

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NetscapeDraftSpec

          This CookieSpec implementation conforms to the original draft
 specification published by Netscape Communications.

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RFC2109Spec

          RFC 2109 compliant CookieSpec implementation.

 class
RFC2965Spec

          RFC 2965 compliant CookieSpec implementation.

Methods in org.apache.http.impl.cookie that return CookieSpec

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RFC2965SpecFactory.newInstance(HttpParams params)

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redpajama_set_name: RedPajamaGithub


can someone tell me where I can find info on pipe flairing? Specifically the dimentions. Do I have to do a bigger flair for R410A than with R22. Can you over flair?
After all the song and dance the manufacturers made over how to joint pipework on R410 systems, they decided to do nothing. The flair is exactly the same as on R22 systems, but they do recommend that a good quality flairing block is used. I use one with a concentric cone and a spring loaded clutch. Must admit it does make a very nice flair every time. Another option are braze on flair ends which are a little more robust than a flaired pipe.
I can only add atype of connection used in places where you need to be sure of no leeks. cost a little but very good.
We call it STEK couplings.
Yup, only need to fit a copper ring inside.
I was looking for something better than the old Imperial flaring block so I imported a Yellow Jacket deluxe flarer from the US because I couldn't find anything local. This produces a beautiful flare. Airefrig now stock a similar Rothenberger unit.
Also in Oz, Heldon are selling high pressure adaptors. To quote "Our swivel adaptors feature a machined stainless steel flare face". "The copper stepped washer fits easily in the stainless steel face, overcoming the issue of misalignment which commonly leads to leakage". You braze these in and they are pressure rated for R410A.


PJ's Coffee & Tea Co. was a 28-year old company native to the New Orleans area when Raving Brands of Atlanta bought it. After the company headquarters relocated out of state, the brand began to lose touch with its roots, which contributed to a drop in sales.
Reconnect the PJ's brand with its New Orleans-based history.
To return to its roots and emphasize its New Orleans connection, Fridge rebranded the company to become PJ's Coffee of New Orleans. A new logo was developed along with a corresponding ad campaign that included POP, collateral, packaging, radio and TV as well as in-store design. All tied back to the story of Phillis Jordan, who opened her first store in 1978, well before the coffee craze. One of the most unique campaign elements was the use of reclaimed antique windows to literally "reframe" the company's history. A unique window was put on display at each PJ's Coffee location.
The new sophisticated design for packaging was awarded the QSR Award for Best Reconnection in 2006 and the QSR/FPI Food Service Packaging Award in 2007. As a result of Fridge's rebranding strategy, PJ's beverage and whole-bean coffee sales increased by over 40%. During Fridge's association with PJ's, the company expanded from 20 stores to over 60 locations throughout the Southeast.


New for 2016, the Croisette armchair is made from an outdoor synthetic fibre that provides you with a comfortable and supported please to sit in your outdoor space.
Dimensions: Height 83cm x Width 65cm.
Chair weave made from a synthetic fibre.


text:
namespace WinPomodoro.Messaging
{
    /// <summary>
    /// A simple message class that we use to pass messages around the application
    /// via the Messenger singleton, but keep the parts sufficiently decoupled
    /// for the MVVM style of work.
    /// </summary>
    public class SimpleMessage
    {
        public SimpleMessage() : this(MessageType.SwitchToTimerView)
        {
        }

        public SimpleMessage(MessageType type)
            : this(type, string.Empty)
        {
        }

        public SimpleMessage(MessageType type, string message)
        {
            Type = type;
            Message = message;
        }

        public enum MessageType
        {
            SwitchToTimerView,
            SwitchToSettingsView,
            SwitchToAboutView,
            SettingsChanged,
            TimerStop,
            TimerStart,
            TimerTick,
            TimerReset
        }

        public MessageType Type { get; set; }

        public string Message { get; set; }

    }
}


text:
Italian Defence Ministry and Alenia Aermacchi sign agreement for three M-346 trainer aircraft
Posted On Monday, 22 December 2014 16:11
World Aviation Defense & Security News - Italy
Alenia Aermacchi and the Italian Defence Ministry today, December 22, have signed a contract worth EUR120 mn ($147 mn) for the supply of three M-346 training aircraft. The agreement also includes a ground base training system and logistic support.
Alenia Aermacchi M-346 Master military trainer aircraft
The M-346 is the ideal platform for a latest-generation integrated training system. Its flexibility means that it can also be configured as an affordable advanced defence aircraft for operational roles. Thanks to its innovative technical characteristics, the aircraft also stands out on account of its high degree of safety and low acquisition and operating costs. The M-346 has been designed using the latest "design-to-cost" and "design-to-maintain" concepts, with an avionics system modelled on those used in new-generation fighter aircraft, such as Eurofighter, Gripen, Rafale, F-22 and the future JSF.
The M-346 Master is powered by two Honeywell F124 engines, and uses an digital flight control system by Teleavio/Marconi in collaboration with BAE System, Dowty and Microtecna for actuators. Armaments are carried on nine external store stations, six of which are underwing, equipped with 450kg-class ejector release units, and two wingtip stations for air-to-air missiles. The under-fuselage station carries avionics pods or a 300 l conformal fuel tank.
Besides Italian air force, the Alenia M-346 has been ordered by Israeli air force (30 ex.), with deliveries started in mid-2014, and by the Republic of Singapore air force, which for the deliveries of 12 M-346 trainers were completed in March 2014.

redpajama_set_name: RedPajamaCommonCrawl


Price for Huawei Honor 7S 16Gb Gold in Riyadh, Jeddah, Khobar (KSA).
Honor 7S Dual SIM - 16GB, 2GB RAM, 4G LTE, Gold. Warranty 2 years.
Honor 7S, 16GB, Gold. Warranty 2 years.
Honor 7S, 16 GB, Gold, 4G LTE. Warranty 2 years.


또한 OnForeColorChanged 메서드를 사용하면 파생 클래스가 대리자를 연결하지 않고도 이벤트를 처리할 수 있습니다.The OnForeColorChanged method also allows derived classes to handle the event without attaching a delegate. 이는 파생 클래스에서 이벤트를 처리하는 기본 방법입니다.This is the preferred technique for handling the event in a derived class.
파생 클래스에서 OnForeColorChanged(EventArgs)를 재정의하는 경우 등록된 대리자가 이벤트를 받도록 기본 클래스의 OnForeColorChanged(EventArgs) 메서드를 호출해야 합니다.When overriding OnForeColorChanged(EventArgs) in a derived class, be sure to call the base class's OnForeColorChanged(EventArgs) method so that registered delegates receive the event.


18 ft ( 3.5m) castoldi tender with 125hp engine, splash guards , bimini top and teak decking. 3.5m tender with 25hp Outboard. 1300cc Waverunner kawasaki 800cc jet ski water skis , wakeboards, towable toys. etc segway seabob APPLE TV with direct access via the net to a never ending range of new releases - Plus over 8000 songs, and more than 100 TV episodes and a great range of films.
APPLE TV with direct access via the net to a never ending range of new releases - Plus over 8000 songs, and more than 100 TV episodes and a great range of films.


text:
Q: IOS/Objective-C/Core-data: how to get object from objectID This answer suggests that to get an object from the objected, you use a method such as this:
-(NSManagedObject *)objectRegisteredForID:(NSManagedObjectID *)objectID

or
-(NSManagedObject *)objectWithID:(NSManagedObjectID *)objectID

This may be a dumb question but how do you call these methods? With the MOC, managed object and other objects involved, am finding this very confusing and not succeeding in calling either method.
This is giving a 'use of undeclared identifier' error, for example:
NSManagedObject *object = objectRegisteredForID:self.moID;

Thanks for any clarification.

A: Yes, both are methods of the NSManagedObjectContext therefore you need to call this method on an NSManagedObjectContext instance where this object is in.
See also https://developer.apple.com/library/mac/documentation/Cocoa/Reference/CoreDataFramework/Classes/NSManagedObjectContext_Class/index.html#//apple_ref/occ/instm/NSManagedObjectContext/objectWithID:


text:
South American Silver Files International Arbitration Against Bolivian Government
South American Silver Corp. (TSX:SAC)(OTCQX:SOHAF) announced today that its wholly-owned subsidiary, South American Silver Limited, has commenced international arbitration proceedings against the government of Bolivia under the Arbitration Rules of the United Nations Commission on International Trade Law pursuant to the Agreement between the Government of the United Kingdom and the Government of Bolivia for the Promotion and Protection of Investments.
The arbitration has been commenced as a result of acts and omissions of the Government including the issuance of Supreme Decree No.1308 on August 1, 2012. The Decree revoked mining concessions covering the Malku Khota Mining Project held by Compania Minera Malku Khota S.A. a wholly-owned subsidiary of South American Silver Limited. These measures resulted in the complete expropriation of the Project without compensation. The arbitration claim is for Bolivia's breach of the Treaty and international law. Bolivia has breached the Treaty's requirement to provide full protection and security to foreign investors and their investments, as well as the Treaty's protections against, among other things, expropriation without just and effective compensation, unfair and inequitable treatment, and less favorable treatment than afforded to Bolivian nationals or nationals of third states.
Since the Decree, the company has made numerous attempts to open a dialogue with the Government with the aim of reaching an amicable resolution of the dispute, including receiving fair-value compensation. In late February 2013, the company was invited to attend a meeting set for 17th April, 2013 in La Paz, Bolivia. Attorneys representing South American Silver attended the April meeting but no resolution was reached and no compensation offered. The Decree stated clearly that the Bolivian state mining company, Corporacion Minera de Bolivia, would hire an independent firm to carry out a valuation of the investments made by CMMK within a period not to exceed one hundred and twenty business days. However, no such valuation has been carried out, nor has an ndependent firm been hired to undertake the valuation.
Exploration commenced at Malku Khota in 2003 and work on the Project continued through the expropriation in August 2012. After completing a total of 42,700 meters of drilling in 121 diamond core holes, and undertaking metallurgical testwork, process development and engineering design, a Preliminary Economic Assessment Update was filed on SEDAR on 13th May, 2011 in a NI 43-101 technical report. The May 2011 PEA Update included economic modeling that indicated pre-tax net present value at a 5% discount rate of:
--  $704 million at metal prices of $18.00/oz silver and $500/kg indium
--  $1.482 billion at $25.00/oz silver and $570/kg indium
--  $2.571 billion at the $35.00/oz silver and $650/kg indium.(1)
After the 2011 PEA Update, the pre-feasibility study phase was started in June 2011. The ongoing work program, which was designed to expand mineral resources and enhance project economics, included the development of social and community programs to benefit indigenous communities, baseline studies for the environmental review and assessment program, exploration, metallurgical testwork, flowsheet development, engineering design and preparations to construct a modern 150 person exploration camp near the exploration site. The planned work programs including the PFS were not completed due to the expropriation.
Phillip Brodie-Hall, President and CEO of South American Silver said, "It is regrettable that we are forced to resort to international arbitration to resolve this matter. Bolivia chose to expropriate our mining concessions; it must now meet its legal obligations and compensate us for this significant loss. International arbitration gives us the means to pursue our case and we are very confident that it will deliver fair value compensation. We have a first-class team working on the case and, while we are open to dialogue with the Government, we are prepared to go the full distance in arbitration if that's what it will take to get fair value compensation. Our loyal shareholders deserve nothing less."
Now that the arbitration has been commenced, the Bolivian Government has thirty days to respond and an arbitration tribunal will be empanelled in the coming months. Thereafter, the tribunal will set a provisional timetable for the arbitration itself including a schedule for submission of a Statement of Claim, Statement of Defense and oral hearings. Unless there is a negotiated settlement of the dispute, it is expected that the arbitration could take up to 2-3 years from commencement of proceedings until rendering of a final award.
Bolivia has been involved in over fifteen "nationalizations", the majority of which have taken place after President Morales came to office. A number of the cases have reached negotiated settlements and at least five others are pending. Bolivia's reserves of cash and gold were estimated at $13.62 billion as of December 31, 2012, up from US$3 billion when Morales came to office in 2006. Bolivia's credit rating was upgraded by all three major rating agencies in 2012, the most recent being Fitch Ratings upgrade from B+ to BB- in October 2012. Bolivia has demonstrated that it has ready access to international funding, having had a $500 million 10-year bond offering significantly oversubscribed in August 2012 at an interest rate of only 4.875%. The IMF recently forecast that Bolivia will be one of the fastest growing economies in South America with an expected GDP growth rate in 2013 of 4.8%.
Mr. Brodie-Hall went on to say, "It is not a question of whether Bolivia is capable of meeting its obligation to compensate the company for the expropriation; it clearly has the financial capacity."
About South American Silver Corp.
South American Silver Corp. is a growth focused mineral exploration company creating value through the exploration and development of the large scale Escalones copper-gold project in Chile, the realization of value from the Malku Khota project in Bolivia and the pursuit of new opportunities. South American Silver Limited is a company incorporated in the Bermuda Islands, an overseas territory of the United Kingdom, which through its wholly owned Bahaman subsidiaries: Malku Khota Ltd, G.M. Campana Ltd. and Productora Limited., owns one hundred per cent of the outstanding shares of Compania Minera Malku Khota S.A., a Bolivian company, holder of the Malku-Khota mining concessions until their nationalization by the Bolivian Government. The Company's approach to business combines the team's track record of discovery and advancement of large projects, key operational and process expertise, and a focus on community relations and sustainable development. Management has extensive experience in the global exploration and mining industry.


text:
Tag Archives | los angeles opera
Singers pay 'electrifying' tribute to opera
By Aryn Plax, Berenice Gonzalez on November 18, 2016 in Arts, Etc., Feature Slider, Music
Los Angeles Opera artists performed an ensemble of songs from plays and operas as part of the Lyceum series Sunday in Morgan Auditorium.
By Kendra Craighead, Tyler Evains on November 11, 2016 in Arts, Etc., Happenings
Campus and community arts events for the week of Nov. 11, 2016.
By Kendra Craighead, Tyler Evains on November 4, 2016 in Arts, Etc., Happenings
Campus and community arts events for the week of Nov. 4, 2016.
By Kendra Craighead on October 28, 2016 in Arts, Etc., Happenings
Summer releases look promising
By Kady Bell on May 12, 2006 in Arts, Etc., Movies, Music
Upon publication of this story, summer will be a short 40 days away. And just as Country Time Lemonade commercials act as a bell in my head – forever ringing in bright blue skies and bathing suits – new album releases, upcoming blockbusters, concert tours and various forms of local entertainment serve as other telltale […]


NWI Food Council Board Member, Dominique Edwards, discusses food insecurity in NWI with attendees at LaPorte County Master Gardeners Brown Bag Series on local food.
The LaPorte County Master Gardeners invited the NWI Food Council to join a panel discussion on local food at their first session of their Brown Bag Series. It was their largest turn out for a Brown Bag Series presentation with a few dozen in attendance, demonstrating strong community interest in the topic of local and sustainable food. Dominique Edwards, board member with the NWI Food Council and food access expert, had the opportunity to speak about the council's work and did a deep dive into the basic knowledge around food systems on a local and regional level. The conversation was enhanced as the panel and audience spoke about food insecurity, food access, food deserts, and food justice. Dominique shared her graduate research on Gary's food deserts and the current situation of food insecurity data in LaPorte County. The series wrapped with a discussion on ways to combat local food system disparities.
Dominique was joined on the panel by Jason from the Pax Center, Damien Appel from Native Roots Farm, and Catherine Mrozinski-Langford from the LaPorte Farmer's Market. We were honored to be a part of this panel!
The NWI Food Council volunteers for County Line Orchard's "Farm to Table" each year to assist chefs with sourcing local ingredients from farms within our region. Our team also spends the evening helping chefs hand out tastings to patrons and working the impressive local cheese, apple and cracker station. It's a wonderful evening with around 500 guests looking to sample the flavors of the season and the region!
Legacy Foundation received a $226,119 grant from the United States Department of Agriculture to address food deserts in Gary and East Chicago. The Foundation partnered with The NWI Food Council, City of Gary Department of Environmental Affairs/Green Urbanism and Lake County Purdue Extension to apply for the USDA Farmers Market Promotion Program to address high rates of poverty and food insecurity in northern Lake County, Indiana.
"Access to healthy food is something many of us take for granted. We can easily drive to the grocery store or visit our neighborhood farmers market and pick up fruits and vegetables, but there are so many in our community who lack the ability to do this. We think that's unacceptable," said Legacy Foundation President, Carolyn Saxton.
Nearly 75,000 people in Lake County are food insecure, according to Map the Meal Gap, a Feeding America study. Food insecurity refers to a lack of access, at times, to enough food for all household members and limited or uncertain availability of nutritionally adequate foods. According to the USDA, there are 24 food deserts in Lake County and 14 of those are in Gary. That means that more than 33% of the population along the census tract lives over a mile from a supermarket or grocery store. The lack of access to nearby food in low-income areas is often worsened by financial and transportation barriers.
The new three-year program will add food markets and mobile markets for residents in Gary and East Chicago and increase the number of farmers markets eligible to accept benefits from the Supplemental Nutrition Assistance Program (SNAP). Currently, there are only two SNAP eligible farmers markets in the city of Gary.
Through this program, new farmers markets will be created in both Gary and East Chicago and capacity will be expanded for a Gary farmers market. All eight farmers markets already operating throughout Lake County will receive capacity building and technical assistance. The project aims to increase consumption of and access to locally grown produce for residents in food deserts. It will expand marketing, outreach, technical assistance, education and local sales for farmers, and create a mobile food market to reach residents in at least half of the 24 food desert census tracts.
In the coming fall and winter seasons, Legacy Foundation and partners will work to get local urban farms eligible to receive SNAP benefits. New farmers markets and the mobile market are slated to begin in the summer of 2019.
In case you hadn't heard: besides getting healthy local food on your child's school lunch plate, the farm-to-school movement is now reaching cafeterias of all kinds! Colleges, pre-schools, prisons, hospitals—food activists around the country are working with local growers to connect the dots in our food economy.
Elias Crim, NWI Food Council board member, had the opportunity to attend this excellent biennial event, hosted by the National Farm to School Network. It brought together a diverse group of food service professionals, farmers, educators, students, representatives from nonprofits and government agencies, public health professionals and more for three days of learning, networking and movement building. Interestingly, the mood was more like that of a political movement than a professional conference!
Keynote speakers included Rodney K. Taylor (a pioneer for establishing the "Farmers' Market Salad Bars" in California schools—back in 1997!) and Haile Thomas (17-year old speaker, health activist, and Food Network chef).
From our NWI area, Josh Craig, media specialist (and F2S activist) at Munster High School also attended. We hope to work with his school—which already has a F2S program underway--in our future NWIFC activities in this area.
The overall impact of these F2C programs is to support local agriculture, address inequities in the food system and foster a culture of food literacy in the community.
Nationally, according to the Farm to School Network, some 42,587 schools (about 42% of all U.S. public schools) now have F2S programs, with 23.6 million students engaged. These schools in 46 states are now spending $789 million on local food.
The latest Indiana Department of Education data indicate that 31% of our state's schools have a farm-to-school program of some kind in place (many states are now well over 50%), with local food representing an average of 18% of the school lunch budget, and 29% of all Indiana schools now offering a school garden program. Lots more work to be done!


Tip #1: Don't just go with the lowest price. Experts agree that the lowest price is not always the best value; in fact, there are many considerations to think about before hiring a contractor for a complete roof replacement. Vancouver, WA is home to many fine roofing contractors. We recommend Soto & Brothers Roofing for repairs and replacements for the quality of workmanship, service, and overall value that they offer their clients. Don't just take our word for it- go online and check out reviews to see what locals have to say about Soto & Brothers. We're convinced, you'll be a believer as well.
Tip #2: Find a contractor that offers free resources and additional value along with their affordable prices. For example, a roofing contractor that offers free estimates and free or low cost inspections will add a lot of benefit to their service. Call Soto & Brothers for your free estimate on a roof replacement in Vancouver, WA. Professional advice is invaluable at a time when you're facing a high investment. Make sure you're getting as much for your investment dollars as possible by working with an agency that champions affordable service.
Tip #3: Discover how reliable your prospective contractor is by calling their office with your questions or sending an email. If the company is reliable, they won't want to keep you waiting any longer than necessary. You'll discover how much worth they place on potential clients by looking at how much time it takes for them to get back with you- and whether they apologize for a lengthy wait. When you bring your list of questions to their experts, do they respond in detail and take the necessary time to address your concerns? The hallmark of a good contractor is how they communicate with prospective clients. Call Soto & Brothers at 360-241-5024 to discuss your upcoming roof replacement in Vancouver, WA- you'll be glad you did.
Tip #4: If time is a factor, ask about guarantees and warrantees on the workmanship and materials. Protect yourself and your investment by hiring a contractor that is clear about what they are promising for the cost. At Soto & Brothers Roofing, you are 100% protected with a guarantee that is among the best in the industry. Feel free to visit their website and see for yourself why locals prefer to do business with Soto & Brothers.
Tip #5: Ask your contractor what type of roof replacement they recommend. Just because your home was protected with shingles does not mean you have to go with the same kind of roof for your replacement. New metal roofing systems offer incredible value and long life for your one time cost and come with little maintenance throughout the years. Request a free quote for your roof replacement in Vancouver, WA by calling Soto & Brothers Roofing at 360-241-5024- their pros will deliver exceptional results at an affordable cost- guaranteed.


text:
Roundtable Staff
Admirals Roundtable
News & Discussion Site For Your Milwaukee Admirals
News	 November 29, 2010 April 10, 2014
Grading the Admirals Through the First Quarter of the Season
Offense (B-): Clearly, coach Lane Lambert has some work to do with Milwaukee in making the Admirals a more dangerous offensive hockey team. Other than Linus Klasen and a few other players, Milwaukee isn't blessed with an abundance of firepower. Therefore the offense has to come by way of committee effort–a Nashville trademark. Prior to the road trip, the Admirals got goals from players on their top three lines, but that dried up some in Texas and Oklahoma. Milwaukee averages 2.55 goals per game, which is not going to cut it long term.
Defense (B+): This might have been the hardest grade for me to judge. 90% of time Milwaukee has really impressed me in their own end, preventing their opponent from getting quality scoring chances against. Allowing just 2.36 goals against per game is a testament to the Admirals efforts in the defensive zone. However, lapses at bad times have proved punishing for Milwaukee.
Goaltending (A-): If this only had to do with the performance of Mark Dekanich, I would state it as an A or A+, but No. 2 slot has hardly been reliable so far for Milwaukee. It's nice to see Jeremy Smith picking up some much needed wins for the Admirals, but Milwaukee really needs Chet Pickard to elevate his game to the higher level he seems more than capable of reaching, but for some reason hasn't found yet.
Special Teams (C-): Carrying a power play ranked 20th in the AHL at 15.8% and penalty kill ranked 18th working at an 82.1% efficiency just isn't going to cut it long term. The power play in particular went cold as the Admirals headed south on the road. For a team that struggles at times to score goals, special teams could prove to be a decisive weapon for Milwaukee competing against more offensively gifted clubs.
Final Grade (A-): Milwaukee has far exceeded my expectations through the first quarter of the season, which is a credit to Lambert efforts and the efforts of the team as a whole. The Admirals were a young and unproven club to start the year, but prior the extended road trip the club was starting to form solid identity as a tough team to play against. But don't mistake young and unproven for a lack of talent. There is plenty of talent in Milwaukee to build a winning hockey club, and so far Lambert has the Admirals heading in the right direction toward to a berth in the AHL playoffs.
So Roundtable, What are your grades for the Admirals so far?
Filed under: Chet Pickard, Final Grade, Lane Lambert, Linus Klasen, Mark Dekanich, Milwaukee Admirals, special teams
Ads defeat Rampage 5-3 in final game of road trip
Home Sweet Home; Admirals Defeat Moose 6-3
9 thoughts on "Grading the Admirals Through the First Quarter of the Season"
TooManyMenonTheIce says:
Yes we are only 4 points out of first and if we were able to pull out a few of those shoot outs early on we would be even closer but I still only give us an overall grade of a B.
(C-) Special team units do need to step it up. I think we were close to having more short handed goals than power play goals on the road trip. That needs to be a bit tighter.
(A) Goaltending is coming around. Good to see Smith stepping up and playing fairly well when needed.
(B-) Offense is lacking but some players are stepping up when others can't seem to find the back of the net.
(B) Defense is holding it's own in our own zone but part of taking care of your own zone is breaking out of it to start the offensive rush. I think I see too much of just chipping it out of the zone and the lack of solid tape to tape passes.
I have to give you an "I" for incomplete on your grade card for the goaltenders. Your analysis of Dekanich is right on but your brief blurb on Jeremy Smith is a weak attempt to down play his early success with Milwaukee. In just 3 games, he has won more games than Pickard did in 14 over the last two seasons. No disrespect intended to Pickard but Jeremy Smith deserves a chance to elevate his game to a higher level too without some star struck fan like yourself lobbying for the return of a goalie whose numbers say "hype without substance". The proof is in the statistics. You owe it to all of us to "check your sources" before you publish. Why would you want to return Pickard to Milwaukee when someone else is outplaying him? Pickard's numbers in Cincinnati are not very impressive which leans more towards "hype without substance" again. What Milwaukee needs is the goaltending to continue on its' current path, no matter who the goalies are. You'd see this if you could wipe the star dust out of your eyes.
jasonkarnosky says:
Mark, in response I would have to say please read the rest of my posts before you go about writing something like this. The way Pickard is playing, no doubt Jeremy Smith deserves a shot and credit to him for taking advantage. But there is a reason why Pickard made the Admirals out of training camp and not Smith.
Maybe Chet Pickard never will figure it out, but the talent is there. I am certainly NOT lobbying to bring back Pickard, in fact at this point Nashville is probably better off trading Pickard to another team and to another goaltending coach who might be able to help him figure things out.
Btw…here is my sources: Jeremy Smith in his three games in an Admirals uniform: 2-1-0, with a 3.34 G.A.A. and a 89.1% Save Percentage. Granted those numbers are better than Pickard's (0-3-1, 3.41 G.A.A. and a 88.5% Save Percentage), but they aren't as impressive as you might think. I would be willing to bet that Smith wishes he was playing better than he has so far to make sure he takes Pickard's job away from him. But neither of those goaltenders screams long term solution if Mark Dekanich gets called up, traded, or hurt, hence the A-.
Chet's Landlord says:
If Chet had five goals of offensive support, he might have a few extra wins too.
The statistics are what they are. Let's let the players prove who should be playing. After 14 games or so, let's look at Smith's stats vs. Pickard's. It's very easy to get caught up in the 1st round pick vs. the 2nd round pick, etc. It seems that Nashville has already done a pretty good job of that all on their own. Pickard has obviously been shown a lighter letter of the law than Smith (probably due to the 1st vs. 2nd round pick scenerio) but even Nashville has to reach a point where they have to second guess their decisions, thus moving Smith up and Pickard down. The facts are that the two most deserving goalies are in Milwaukee right now, and as we all know this can change at any time but until it does…….Let's give Smith the same opportunity as Pickard has had with Milwaukee. 15 plus games in a patient environment focused on development. FYI- Landlord -Milwaukee's offensive support for Smith has averaged 3.3 per game in his 3 starts.
We wish both goalies the best. And we'll love the one we're with. Hopefully Smith will continue to work hard and improve in practice so that he'll have the confidence of the coaches to play him more often than one game in 3-in-3 sets.
Some random guy says:
Something's not right with Chet. I can't put my finger on it but he seems like he is not carrying himself on the ice the same way he was in the WHL. I don't know if it the organizations handling of him or if he is just getting passed up. I hope he gets right for his and the organizations sake but if not I hope he gets traded and finds a better fit like Jones and Santorelli from last year.
I think Jeremy has done well since the call up and it will be a battle for the two young men as backup. I haven't seen him play prior to this year so I don't know how well he is progressing but so far so good.
Chris in NOLA says:
Goaltending: A. While we can discuss Chet/Smith, whatever. Fact is, without Dex, we wouldnt have anywhere close to the number of wins so far. (Or shootout losses; I kid)
Defense: B. If Aaron Johnson wasn't taking his obligatory 2 penalties a game, I would even consider giving an A. Looks like he's stopped taking them but then again, I haven't paid much attention to the road trip
Offense: C. Do we even have offense besides Klasen? I would say I expected more out of Bourque, but at the same time, he's so young obviously needs time to adapt the the pro level.
Maybe its the fact we don't have anyone who seems to consistently put the puck in the net, but this team has done nothing for me this year to get excited. I would say Josi has been impressive, but we've only seen what, 2 home games out of him?
adsfan says:
B overall. The PP stinks, the PK is okay. Offense B-, Defense B+, Dex A-. Johnson took a stupid penalty last night that allowed the Meese to score their third goal.
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So many of the people I encounter, either in the Church or in the university setting, push themselves hard, holding themselves and others to a standard so high that it encourages an obsessive drive for perfection.
Last weekend, my body sent me a clear message to stop this insane perfectionism. Is it so hard to be content with doing our very best, and praying to do better? We're human, and humans make mistakes, don't always get things done, offend others when it's the last thing intended.
This line from Anne Lamott's newest book, Imperfect birds, stands out, and I'll share it here: "Rae had once made a room-sized weaving for Audubon's Bolinas Lagoon Preserve, of egrets and herons nesting in redwood trees, and Elizabeth remembered now the secret ribbon woven into one branch, which bore the words of Rumi: 'Each has to enter the nest made by the other imperfect bird.'"
This passage leaped into my mind when I learned yesterday that our bishop has asked me to preach at this week's clergy conference. If there's any more intimidating setting, I don't know what it is. At the recent Chrism mass, I recall thinking as I watched a colleague ascend the steps to the pulpit, "Glad it's not me! Tough crowd!"
Ha. So it is that many of us take our turn, at least one of us each year when we come together at the retreat center. So, if you're reading this, please offer up a prayer that the word of God, the mystery of God, and the love of God may be revealed through my speaking.