text:
package org.wso2.carbon.is.migration;

import org.apache.commons.logging.Log;
import org.apache.commons.logging.LogFactory;
import org.wso2.carbon.identity.core.migrate.MigrationClient;
import org.wso2.carbon.identity.core.migrate.MigrationClientException;
import org.wso2.carbon.is.migration.config.Config;
import org.wso2.carbon.is.migration.util.Constant;

import java.util.List;

/**
 * MigrationClientImpl is the one that trigger by the relevant component trigger to start the migration service.
 *
 */
public class MigrationClientImpl implements MigrationClient {

    private static final Log log = LogFactory.getLog(MigrationClientImpl.class);

    @Override
    public void execute() throws MigrationClientException {

        try {

            Config config = Config.getInstance();

            if(!config.isMigrationEnable()){
                return;
            }

            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");
            log.info("............................................................................................");

            VersionMigrationHolder versionMigrationHolder = VersionMigrationHolder.getInstance();
            List<VersionMigration> versionMigrationList
                    = versionMigrationHolder.getVersionMigrationList();

            log.info("Migration Versions List.........................");
            for (VersionMigration versionMigration : versionMigrationList) {
                log.info(versionMigration.getPreviousVersion() + " to " + versionMigration.getCurrentVersion());
            }

            boolean isMigrationStarted = false ;

            for(VersionMigration versionMigration : versionMigrationList){
                log.info(Constant.MIGRATION_LOG + "Start Version : " + versionMigration.getPreviousVersion() + " to "
                         + versionMigration.getCurrentVersion());
                if(!isMigrationStarted && versionMigration.getPreviousVersion().equals(config.getCurrentVersion())){
                    versionMigration.migrate();
                    isMigrationStarted = true ;
                    continue;
                }

                if(isMigrationStarted){
                    versionMigration.migrate();
                    if(versionMigration.getCurrentVersion().equals(config.getMigrateVersion())){
                        break;
                    }
                }
            }
            log.info(Constant.MIGRATION_LOG + "Execution was done through all the requested version list without "
                     + "having unexpected issues. There may be some steps that is not executed correctly but bypass "
                     + "that because of enabling 'continueOnError' property. Please see the above logs to more "
                     + "details.");
        } catch (Throwable e) {
            log.error("Migration process was stopped.", e);
        }

        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");
        log.info("............................................................................................");

    }

}


September 7, 2018 (FPRC) -- With more than a 32% increase in traffic to their website, this Memphis, TN property management company is filling vacancies faster and working more efficiently. This is welcome news to the bottom line of the current clients of Lubin.
After updating their website, creating new content, and putting a strategic effort in place regarding their search engine marketing, Lubin Property Management is delivering some real-world benefits to the owners of the properties that they manage.
With an increased amount of renters and property owners finding their website, this has directly translated to more inquiries and showings of vacant rentals. With rentals getting more exposure and having more showings, the ultimate result is having rentals filled more quickly and more money in property owners pockets.
'Lubin is dedicated to investing in the technology and online strategies that will yield greater returns to our property owner clients. We know that by making this investment, our service level will remain top-notch and our clients will continue to be thrilled with our efforts' says Landon Murie, a member of the marketing team for Lubin.
Lubin's website features pages that are dedicated to available rental properties, showcasing these front and center for prospective renters who are searching online. Lubin hopes that this feature, coupled with the 32% increase in website traffic, will be driving up returns for clients of Lubin for months and years to come.


text:
Double road fatality in southwest Victoria
A teenage girl and a woman have died in a two-car crash at Mount Richmond in southwest Victoria.
Emergency services responded to the head-on collision between a Jeep Cherokee and a Holden Trax on the Portland-Nelson Road at about 10.25am on Thursday.
Victoria Police said the driver of the Holden, a 16-year-old girl and the front seat passenger, a 43-year-old woman, both from Mount Richmond, died at the scene.
A 15-year-old girl in the rear passenger seat sustained life-threatening injuries and was airlifted to hospital.
The driver of the Jeep, a 50-year-old man, sustained non-life-threatening injuries and was taken to hospital in a serious but stable condition.

redpajama_set_name: RedPajamaCommonCrawl


text:
using Newtonsoft.Json;

namespace RKon.Alexa.NET46.Payloads
{
    /// <summary>
    /// Payload for EnpointLowPower Errors
    /// </summary>
    public class EndpointLowPowerErrorPayload : ErrorPayload
    {
        /// <summary>
        /// percentageState value, which an integer between 0 and 100 that indicates the percentage of battery remaining for the device.
        /// </summary>
        [JsonProperty("percentageState")]
        [JsonRequired]
        public int PercentageState { get; set; }
    }
}

redpajama_set_name: RedPajamaGithub


Nancy Buhagiar is an established TV Personality that has been presenting TV Programmes and promoting the use of World Marketing systems since 1992.
Today Nancy and her team prepare delicious recipes as well as makes use of all that World Marketing has to offer so that the true benefits are achieved.
For Recipe Updates and handy information visit Nancy Home Page on Facebook .
Get Notified with our events.
The World Marketing Facebook Page offers some intesteing updates. We urge you to have a look and once you are there like our page. We appreciate your feedback and comments.
Join the World Marketing Club. You will be invited for events and activites that will be organised from time to time .
Follow any one of our product specific pages to have a more detailed perspective on a particular product of system.
Register Your Product and you will be able to benefit further from events activities and promotions that will be organised from time to time.


From the heart of the Potteries in Longton, Stoke on Trent, Walker's Nonsuch is one of Britain's oldest makers of traditional toffee. From humble beginnings they have grown to be a favourite all around the world.
Serving the sweet tooth of local people, Edward Joseph Walker, a former potter, and his sister Florence opened a tiny sweet shop in Longton. He began making toffee in the late 19th century and developed his own special recipe which he kept a closely guarded secret. It was such a success that he went on to open a small factory to meet the ever-growing demand for his tasty toffee.
When his son, Edward Victor joined the family business in 1915 they continued to make delicious toffee to the original recipe and as the business grew Walker's Nonsuch was launched and became a limited company in 1922. As time passed the industrious toffee makers flourished and moved to larger premises.
In 1962, ambitious brothers Ian and Edward took over and set about expanding. With new equipment and the special family recipe, the company's reputation grew from local to national and then to international. In 1981 Edward sadly passed away leaving Ian at the helm for 36 years until his death in December 2017 after completing 60 years of service.
Today the Managing Director is Adrian Hill who has worked at the company for 34 years. Working alongside him are Ian's daughters Katie and Emma along with nephew Edward who are key members of the team along with Jonathan, Jamie and Jessica, the latest generation to join.


The Global Automotive Electric Cable Market report provides an in-depth analysis of the key market insights, its future trends and developments, profiles of leading players, key restraints and drivers, Automotive Electric Cable market segmentation and forecasting. The report highlights the market size and CAGR of the important segments, thus providing quick relevant information of the global Automotive Electric Cable market. This market research report also provides a brief summary of the global Automotive Electric Cable market. The report consists of several aspects that affect the growth of the Automotive Electric Cable market.
Global Automotive Electric Cable Market Analysis Report includes top manufacturers Sumitomo Electric Industries (Japan), Yazaki (Japan), Aptiv (USA), Magneti Marelli (Italy), Hitachi Metals (Japan), Furukawa Electric (Japan), Leoni (Germany), Sumitomo Wiring Systems (Japan), ITT (USA), PKC Group (Finland), Gentherm (USA), A.E.C. (Italy), ACOME (France) along with their company profile, growth aspects, opportunities, and threats to the market development. This report presents the industry analysis for the forecast timescale. An up-to-date industry details related to industry events, import/export scenario, market share is covered in this report.
Chapter 13, 14 and 15 To describe Automotive Electric Cable sales channel, distributors, traders, dealers, Research Findings and Conclusion, appendix and data source.
The report will make detailed analysis mainly on below questions and in-depth research on the development environment, market size, development trend, operation situation and future development trend of Automotive Electric Cable on the basis of stating current situation of the industry in 2019 so as to make comprehensive organization and judgment on the competition situation and development trend of Automotive Electric Cable Market and assist manufacturers and investment organization to better grasp the development course of Automotive Electric Cable Market.
Production Analysis – Production of the Automotive Electric Cable is analyzed with respect to different regions, types and applications. Here, price analysis of the various Automotive Electric Cable market key players is also covered.
Sales and Revenue Analysis – Both, sales and revenue are studied for the different regions of the Automotive Electric Cable market. Another major aspect, price, which plays important part in the revenue generation, is also assessed in this section for the various regions.
Supply and Consumption – In continuation with sales, this section studies supply and consumption for the Automotive Electric Cable market. This part also sheds light on the gap between supply and consumption. Import and export figures are also given in this part.
Competitors – In this section, various Automotive Electric Cable industry leading players are studied with respect to their company profile, product portfolio, capacity, price, cost, gross Margin and revenue.
Other analyses – Apart from the aforementioned information, trade and distribution analysis for the Automotive Electric Cable market, contact information of major manufacturers, suppliers and key consumers is also given.


text:
Q: Doctrine order by relation table with limit I have two tables "series" and "programs" with programs being many to one one series.
Series table
id | name
--------------
1  | lorem
2  | ipsum
3  | foo
4  | bar

Programs table
id | name      | series_id
---------------------
1  | program1  | 1
2  | program2  | 2
3  | program3  | 3
4  | program4  | 4
5  | program5  | 1
6  | program6  | 2
7  | program7  | 3
8  | program8  | 4
9  | program9  | 1
10 | program10 | 2
11 | program11 | 1
12 | program12 | 2

I would like in Doctrine (1.2) to get the two series with the latest program, in this case series 2, followed by series 1, see the last two rows in programs.
My guess would be:
$q = Doctrine_Query::create()->from('Series s')
                             ->leftJoin('s.Programs p')
                             ->orderBy('p.id desc')
                             ->limit(2);

but this returns series 4 and series 3. The problem is that Doctrine uses a distinct query getting the id's before doing the query where the data will be included, they use that tactic, as MySql cannot use limit in subqueries i think.
My questions, how would you get series 2 and series 1 via DQL?

A: try this:
$q = Doctrine_Query::create()
    ->select('s.name')
    ->addSelect('(SELECT p.series_id FROM Programs p WHERE p.series_id = s.id) as series_name')
    ->from('Series s');
    ->orderBy('p.id desc')
    ->limit(2);

redpajama_set_name: RedPajamaStackExchange


In the movie Philadelphia, Tom Hank's character first meets his lawyer, Denzel Washington, as Denzel is on the phone with a potential client who wants to sue after stepping into a clearly marked hole in a city street; Denzel suggested he had a case. But, holes in streets are not the same as holes in parking lots. In this case, the trial court concluded the plaintiff could sue for an injury caused by a hole in a public parking lot. The court of appeals disagreed and held a city is immune from suit for injuries occurring in a public parking lot that services a public golf course. Colorado waives governmental immunity for personal injuries, and thus subjects cities to lawsuits for injuries caused by dangerous conditions in any park or recreation area, public streets, or public buildings. A parking lot is none of these, so the city was immune from suit. Plaintiff's case was dismissed.
A nine year old elementary school student suffered a compound fracture of her arm when she fell off a "zip-line" that was part of the public school's playground equipment. The child and her parents sued. The school claimed, and the trial court agreed, that it was immune from the claim as a governmental entity. However, public entities can be sued if injuries result from a dangerous condition of a "public facility" maintained by a public entity. The court of appeals concluded, apparently for the first time, that public school playground equipment was a "public facility." It was "public" because it was visible to all and not secluded. It was a "facility" because it was man-made, a mechanical device, and installed for children to play upon. The school, therefore, was not immune to a "public facility" claim. The school was, however, immune from the negligent supervision claim.


Soon after Beverly Weston (Sam Shepard) hires a young Native American woman to look after his wife, Violet (Meryl Streep), who has mouth cancer, he goes missing. His daughters (Julia Roberts, Julianne Nicholson, Juliette Lewis and their families come home to provide support their mother, a woman who is non-stop nasty, possibly due to her addiction to prescription drugs.
After the funeral, during dinner with her extended family, she can't stop criticizing everyone at the table and finally, she pushes too far, prompting her eldest daughter Barbara to take action. Over the course of the next few days, dark family secrets come to light and shocking revelations are made.
This dark comedy is based on the Pulitzer Prize award-winning play by Tracy Letts.


text:
About MCB
DNA DYNAMICS AND CHROMOSOME STRUCTURE
Chromatin Association of Human Origin Recognition Complex, Cdc6, and Minichromosome Maintenance Proteins during the Cell Cycle: Assembly of Prereplication Complexes in Late Mitosis
Juan Méndez, Bruce Stillman
Juan Méndez
Bruce Stillman
DOI: 10.1128/MCB.20.22.8602-8612.2000
Evidence obtained from studies with yeast and Xenopusindicate that the initiation of DNA replication is a multistep process. The origin recognition complex (ORC), Cdc6p, and minichromosome maintenance (MCM) proteins are required for establishing prereplication complexes, upon which initiation is triggered by the activation of cyclin-dependent kinases and the Dbf4p-dependent kinase Cdc7p. The identification of human homologues of these replication proteins allows investigation of S-phase regulation in mammalian cells. Using centrifugal elutriation of several human cell lines, we demonstrate that whereas human Orc2 (hOrc2p) and hMcm proteins are present throughout the cell cycle, hCdc6p levels vary, being very low in early G1 and accumulating until cells enter mitosis. hCdc6p can be polyubiquitinated in vivo, and it is stabilized by proteasome inhibitors. Similar to the case for hOrc2p, a significant fraction of hCdc6p is present on chromatin throughout the cell cycle, whereas hMcm proteins alternate between soluble and chromatin-bound forms. Loading of hMcm proteins onto chromatin occurs in late mitosis concomitant with the destruction of cyclin B, indicating that the mitotic kinase activity inhibits prereplication complex formation in human cells.
The molecular mechanism that restricts firing of origins of replication to once per cell cycle invokes the ordered binding to and/or release of different replication proteins from specific DNA sequences (replicators) located in the vicinity of the actual origins of DNA replication. Following separation of sister chromatids at mitosis and during the subsequent G1 phase, prereplication complexes (pre-RCs) are formed at origins of DNA replication. Initiation of DNA replication is triggered by the action of at least two sets of protein kinase activities, cyclin-dependent kinases (CDKs) and Dbf4p-Cdc7p. After initiation, the protein complex at each origin changes to a postreplication state (post-RC), thereby preventing further initiation events for the rest of the cell cycle (reviewed in references 15 and57).
The origin recognition complex (ORC), a six-subunit initiator protein (2), is present in both pre- and post-RCs (10), and one of its functions is to mark the position of replication origins in the genome. The pre-RC is established by the regulated binding of additional factors, which include Cdc6p and the minichromosome maintenance (MCM) proteins. Yeast CDC6 displays a genetic interaction with the ORC and is a critical factor for establishing the competence of replication origins once per cell cycle (12, 33, 47,48). Besides its function in DNA replication, it may also be involved in a mitotic checkpoint control, because Cdc6-deprived yeast cells that do not replicate DNA still undergo a reductional mitosis (4, 47, 63).
Cdc6p is a member of the large AAA+ superfamily of ATPases, which includes Orc1p, Orc4p, Orc5p, MCM, proteins and replication factor C (42). Based on sequence similarities between Cdc6p, replication factor C, and other AAA+ family members and on the characterization of a dominant-negative CDC6 mutant, it has been proposed that yeast Cdc6p might function as an ATP-dependent MCM protein loader (45, 63). Indeed, the association of MCM proteins with chromatin is dependent on Cdc6p (1, 12, 34, 59). Biochemical studies with Xenopus provided additional support for the idea of Xenopus Cdc6p (XCdc6p) being an essential factor for establishing pre-RCs. In Xenopus egg cell extracts, XCdc6 could bind to chromatin only in the presence of XOrc2, and it was absolutely required for the subsequent loading of XMcm3 (6).
Yeast Cdc6p is a highly unstable protein, and many factors seem to be involved in its degradation, including theCDC4-CDC34-CDC53-Skp1 pathway (13, 14, 54). However, ectopic expression of Cdc6p in G2 cells is not deleterious for the cell, and it has been shown that Cdc6p cannot induce MCM protein binding to chromatin at this point unless CDKs are inactivated (9,59). Interestingly, a dominant gain-of-function allele ofCDC6 causes persistent MCM protein binding to chromatin and overreplication of the genome in a single cell cycle (34).
Cdc18+ is the Schizosaccharomyces pombehomologue of CDC6 and performs similar functions in regulating initiation of DNA replication and possibly entry into mitosis (28, 40, 43). Gross overexpression of Cdc18+ results in repeated rounds of DNA replication in the absence of mitosis (23, 43). p65Cdc18 is also a very labile protein targeted for destruction by CDK phosphorylation after cells enter S phase (24). At least in S. pombe and Xenopus, another protein, called Cdt1, participates in the assembly of pre-RCs (37, 44).
MCM proteins are essential for DNA replication (reviewed in reference27). A subcomplex of human MCM proteins may function as a replicative helicase (22). This idea has been recently reinforced by the finding that yeast MCM proteins are required for replication fork progression (32) and by the characterization of a processive helicase activity in an archaeal MCM protein (5, 29).
The regulation of initiation of replication in mammalian cells is much less understood, mainly for two reasons: discrete replicator sequences have not been defined, and the information regarding mammalian proteins involved in this process has been limited. The recent identification of human homologues of ORC subunits (18, 49, 50, 62), Cdc6 (65), and MCM proteins (reference 27 and references therein) suggests that the overall mechanism for replication initiation is conserved between mammals and yeast.
We have therefore investigated the dynamics of chromatin association of endogenous human Orc2, Cdc6, and Mcm proteins (referred to as hOrc2p, hCdc6p, and hMcm proteins) throughout the cell cycle. We used centrifugal elutriation as a method to synchronize cells without interfering with their normal metabolism, and we raised new monoclonal anti-hCdc6p antibodies and polyclonal anti-hMcm antibodies. Our data show that a fraction of hCdc6p is targeted to chromatin during the entire cell cycle and help to define the point at which the hMcm proteins are loaded onto chromatin during late mitosis. We also report a previously unrevealed regulated destruction of hCdc6p and discuss the possible execution points for hCdc6p during the cell cycle.
Antibodies.To generate anti-hCdc6p monoclonal antibodies, full-length hCdc6p was overexpressed inEscherichia coli and purified as a glutathioneS-transferase (GST) fusion protein. After cleavage of the GST moiety, hCdc6p was further purified in a Prep Cell gel (Bio-Rad) and used as an immunogen. Standard techniques for immunization of mice, analysis of test bleeds, generation of hybridoma cell lines, and production of ascitic fluid were used (19). A double screening of hybridomas was done by dot blotting, using either native or denatured hCdc6p as the antigen immobilized to nitrocellulose. Hybridoma cell lines hCdc6-26 and hCdc6-37 were positive in both tests. Several other positive cell lines (hCdc6-34, hCdc6-39, and hCdc6-41) turned out to produce immunoglobulin Ms (IgMs) and were not used in this work (unpublished data). Anti-hOrc2p and anti-hCdc6p polyclonal antibodies have been described before (18, 65). Different anti-hMcm3p, anti-hMcm4p, and anti-hMcm5p polyclonal sera were raised in rabbits against synthetic peptides corresponding to amino acids 674 to 693 of hMcm3p, amino acids 21 to 40 of hMcm4p, and amino acids 19 to 38 of hMcm5p, conjugated to keyhole limpet hemocyanin. Anti-pan-Mcm polyclonal serum was raised against a synthetic peptide that is highly conserved in all MCM proteins (amino acids 405 to 421 of hMcm3p). Antibodies against TFIIB were a gift from N. Hernandez (Cold Spring Harbor Laboratory). Monoclonal antibody 12CA5 (antihemagglutinin [anti-HA]) was obtained from C. Bautista (Cold Spring Harbor Laboratory). Anti-MEK2 (M24520) was obtained from Transduction Laboratories, anti-cyclin A and anti-p53 were obtained from Santa Cruz Biotechnology, and Texas Red-conjugated anti-rabbit IgG was obtained from Jackson Immunoresearch Laboratories, Inc.
Cell manipulations and centrifugal elutriation.Cell lines HeLa, U2OS, 293, Manca (B-cell lymphoma), and Raji (Burkitt lymphoma) were obtained from the Cold Spring Harbor Laboratory cell culture facility or the American Type Culture Collection. Separation of logarithmically growing cells into distinct cell cycle phases was accomplished by centrifugal elutriation in a Beckman J2-21 M centrifuge and a JE-6B rotor with a large (40-ml) separation chamber. The rotor was kept at a speed of 1,500 rpm, the temperature was 20°C, and the medium flow was controlled with a Cole-Parmer Masterflex pump. Consecutive fractions of 250 ml were collected at increasing flow rates, ranging from 40 to 120 ml/min. Cells in all fractions were counted, and extracts were prepared after normalization by cell number. For cytofluorometric analyses, an aliquot of 106 cells was fixed by rapid submersion in 1 ml of ice-cold 90% ethanol. After at least 1 h of fixation at 4°C, cells were collected by centrifugation and incubated for 30 min at 37°C in 0.5 ml of staining solution (25 μg of propidium iodide per ml and 10 μg of RNase per ml in phosphate-buffered saline [PBS]). Stained cells were analyzed on a Becton-Dickinson FACScan.
To synchronize a cell culture in prometaphase, 50 ng of nocodazole per ml was added to 1 liter of HeLa cells growing in suspension medium (Joklik's modification to minimum essential medium supplemented with 10% calf serum). After 25 h, the culture was centrifuged at low speed (5 min at 3,000 rpm in a Beckman CS-6R centrifuge), washed with PBS, and resuspended in prewarmed fresh medium without nocodazole. Every 20 min after the release, an aliquot was taken from the main culture, and the cells were collected, washed once in PBS, and used to prepare total cell extracts or subjected to biochemical fractionation as described below. A total of 106 cells from each fraction were used for cytofluorometric analysis of DNA content.
Total cell extracts, nuclear extracts, and chromatin isolation.To prepare total cell extracts, tissue-cultured cells were harvested by centrifugation, washed in PBS, and directly resuspended in Laemmli buffer, followed by sonication for 15 s in a Tekmar CV26 sonicator set at 25% amplitude. To prepare nuclear extracts, the cells were washed once with PBS and lysed by Dounce homogenization in hypotonic buffer (20 mM Hepes-KOH [pH 8.0], 5 mM KCl, 1.5 mM MgCl2, 5 mM Na butyrate, 0.1 mM dithiothreitol [DTT]). Nuclei were collected by centrifugation (10 min, 16,000 × g, 4°C) and resuspended in nuclear extraction buffer (15 mM Tris-HCl [pH 7.5], 1 mM EDTA, 0.4 M NaCl, 10% sucrose, 1 mM DTT). After 30 min on ice, insoluble proteins were removed from the nuclear extract by high-speed centrifugation (40 min, 100,000 × g, 4°C).
To isolate chromatin, cells were resuspended (4 × 107cells/ml) in buffer A (10 mM HEPES, [pH 7.9], 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM DTT, 5 μg of aprotinin per ml, 5 μg of leupeptin per ml, 0.5 μg of pepstatin A per ml 0.1 mM phenylmethylsulfonyl fluoride). Triton X-100 (0.1%) was added, and the cells were incubated for 5 min on ice. Nuclei were collected in pellet 1 (P1) by low-speed centrifugation (4 min, 1,300 × g, 4°C). The supernatant (S1) was further clarified by high-speed centrifugation (15 min, 20,000 × g, 4°C) to remove cell debris and insoluble aggregates. Nuclei were washed once in buffer A, and then lysed in buffer B (3 mM EDTA, 0.2 mM EGTA, 1 mM DTT, protease inhibitors as described above). Insoluble chromatin was collected by centrifugation (4 min, 1,700 × g, 4°C), washed once in buffer B, and centrifuged again under the same conditions. The final chromatin pellet (P3) was resuspended in Laemmli buffer and sonicated for 15 s in a Tekmar CV26 sonicator using a microtip at 25% amplitude (see Fig. 3A). To release chromatin-bound proteins by nuclease treatment, cell nuclei (P1) were resuspended in buffer A plus 1 mM CaCl2 and 0.2 U of micrococcal nuclease (Sigma). After incubation at 37°C for 1 min, the nuclease reaction was stopped by the addition of 1 mM EGTA. Nuclei were collected by low-speed centrifugation and lysed according to the chromatin isolation protocol described above.
Immunoblots, immunoprecipitation, and immunofluorescence.Standard protocols for immunoblots, immunoprecipitation, and immunofluorescence were used (19), with the following notations. Proteins were blotted to nitrocellulose membranes for 1 h at 0.5 A using the buffer described by Bolt and Mahoney (3) (40 mM Tris, 20 mM sodium acetate, 2 mM EDTA, 20% [vol/vol] methanol, 0.05% sodium dodecyl sulfate [SDS]). For immunofluorescence, HeLa cells growing on coverslips were fixed with 3% paraformaldehyde in PBS (pH 7.4) for 15 min at room temperature and permeabilized with 0.2% Triton X-100 in PBS for 5 min at 4°C. Cells were incubated with PBS containing 1% normal goat serum as a blocking agent for 30 min and then with anti-hMcm4p antibody (1:200 in PBS–1% normal goat serum) for 1 h. After being washed three times for 5 min with PBS, the cells were incubated with Texas Red-conjugated anti-rabbit IgG (from goat) for 1 h. DNA was stained with 1 μg of Hoechst stain per ml for 30 s.
Proteasome inhibition and in vivo ubiquitination assays.HeLa cells (106) were treated for 12 h with protease inhibitor LLnL, LLM, MG132, or β-lactone or proteasome inhibitor I (PSI) (all inhibitors were from Calbiochem) at a concentration of 25 μM. Cells were then harvested and extracts were prepared exactly as described by Salghetti et al. (53). Ubiquitinated intermediates in human cells were detected using the His6-tagged-ubiquitin (His-Ubi) method of Trier et al. (61) as used by Salghetti et al. (53). HeLa cells (106) were transfected with 2 μg of pCGN.CSH.FL42 (which encodes HA-tagged hCdc6p), pCGN.Myc, or pCGN.TFIIB, either in the absence or in the presence of plasmid pMT107, which encodes His-Ubi (a gift from D. Bohmann, EMBL, Heidelberg, Germany). At 24 h posttransfection, 20 μM MG132 was added to the cells, when indicated. Cells were harvested at 36 h posttransfection, and His-tagged (therefore, ubiquitinated) proteins were purified on Ni-nitrilotriacetic acid–agarose and subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE). HA-tagged proteins were detected by immunoblotting with anti-HA (12CA5) antibody.
Generation and characterization of monoclonal anti-hCdc6p antibodies.To study the regulation of CDC6 in mammalian cells, monoclonal antibodies against full-length recombinant hCdc6p were raised (see Materials and Methods). Two monoclonal antibodies (hCdc6-26 and hCdc6-37) were characterized in detail and used throughout this study. In immunoblots, both antibodies recognized a protein of approximately 62 kDa in protein extracts from human cells (Fig. 1A). In some experiments, a second band of slightly lower molecular mass was also detected, most likely corresponding to a modified form or a degradation product of hCdc6p. The specificities of both hCdc6-26 and hCdc6-37 in immunoblots were equal to or better than those of several anti-hCdc6p polyclonal sera tried (Fig. 1A and data not shown). The new antibodies efficiently recognized recombinant hCdc6p expressed in E. coli or baculovirus-infected insect cells (not shown).
Specificities of new monoclonal anti-hCdc6p antibodies (Ab). (A) Nuclear extracts from asynchronous 293 cells were subjected to SDS-PAGE, and proteins were transferred to nitrocellulose. After protein staining with Ponceau-S red, individual lanes were cut and immunoblotted with the indicated dilution of monoclonal antibody hCdc6-26 or hCdc6-37 or a polyclonal antibody, anti-hCdc6p. A major signal corresponding to a 62-kDa protein was detected in all cases. (B) Immunoprecipitation (I.P.). Fifty microliters of nuclear extract from 293 cells (0.5 mg of total protein) was incubated for 1 h with 1 or 3 μl of ascitic fluid of the corresponding monoclonal antibody or with 3 μl of an unrelated control antibody (lane C). Immunocomplexes were purified with protein G-Sepharose 4B, subjected to SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-hCdc6p polyclonal antibodies. Lane I, 10% of the input sample. (C) Epitope mapping. Constructs expressing different C-terminal deletions and one N-terminal deletion of hCdc6p were made and expressed in E. coli as GST fusion proteins. Monoclonal antibodies hCdc6-26 and hCdc6-37 were used to detect the different truncated proteins by immunoblotting. The asterisks represent consensus sites for CDK phosphorylation. Boxes A and B indicate the position of Walker A and Walker B motifs, required for ATP binding and/or hydrolysis. The gray box marked Leu represents the hCdc6p leucine zipper. The regions that contain the epitopes recognized by hCdc6-26 and hCdc6-37 are indicated with brackets. aa, amino acid.
The antibodies also recognized hCdc6p in its native form, as shown by immunoprecipitation from human nuclear protein extracts: both hCdc6-26 and hCdc6-37, but not a control antibody, immunoprecipitated a 62-kDa protein, which was subsequently identified as hCdc6p by immunoblotting with a polyclonal antibody (Fig. 1B).
Monoclonal antibodies hCdc6-26 and hCdc6-37 recognize different epitopes in hCdc6p, as indicated by their ability to recognize in immunoblots a series of deletion derivatives of hCdc6p (Fig. 1C). The hCdc6-26 epitope is located between amino acids 100 and 125 of hCdc6p (possibly overlapping with Ser106, a known phosphorylation site [26]), and the hCdc6-37 epitope is located between amino acids 150 and 200. Within this region, amino acids 170 to 178 display the highest score for hydrophilicity and surface probability. This region does not contain any potential phosphorylation sites. The experiments included in this work have been performed with both monoclonal antibodies, and the results with hCdc6-37 are shown.
Association of hOrc2, hCdc6p, and hMcm proteins with chromatin.After biochemical fractionation of human cells, hCdc6p was recovered preferentially in nuclear extracts and not in cytosolic extracts (65). We have investigated whether hCdc6p associates with chromatin, because in yeast and Xenopus, the presence of the ORC and Cdc6p on chromatin is a prerequisite for the subsequent loading of MCM proteins. A simple chromatin-binding assay was developed for this purpose (Fig. 2A) (see Materials and Methods for details). In brief, cells were lysed with Triton X-100 in a sucrose-rich buffer. Nuclei were collected by low-speed centrifugation, washed, and then lysed for 30 min in a no-salt buffer. A second centrifugation step separated remaining soluble nuclear proteins from an insoluble fraction. Proteins found in the final pellet were likely to be bound to chromatin or the nuclear matrix.
Chromatin binding of initiation proteins. (A) Scheme of the biochemical fractionation method. See Materials and Methods for details. (B) An asynchronous culture of Raji cells was subjected to the biochemical fractionation described in panel A. After cell lysis, the nuclei were divided in two aliquots. One of them was incubated for 1 min at 37°C with 0.2 U of micrococcal nuclease, and the other one was incubated in the same conditions without nuclease. After this treatment, nuclei were lysed and the solubilized nuclear proteins (S3) were separated from the chromatin-bound proteins (P3) by centrifugation. The distributions of different proteins in the total cell extract (TCE) soluble fraction (S2), solubilized nuclear proteins fraction (S3), and chromatin-nuclear matrix-bound fraction (P3) are shown.
The distribution of several proteins in the different fractions was tested after biochemical fractionation of an asynchronous cell culture of Raji cells (Fig. 2B). hOrc2p and hCdc6p were recovered preferentially in the chromatin-nuclear matrix fraction (P3). A small fraction of hCdc6p was detected in the soluble cytosolic (S2) and nucleoplasmic (S3) fractions. In contrast to hOrc2p and hCdc6p, hMcm3p was split between the soluble (S2) and chromatin-bound fractions. On the other hand, MEK2, a cytosolic kinase involved in signal transduction, was recovered exclusively in the S2 fraction, indicating that the extraction of cytosolic proteins after cell lysis was complete. The hOrc2, hCdc6, and hMcm3 proteins present in the final insoluble fraction were solubilized almost completely by treatment of the nuclei with micrococcal nuclease (see Fig. 4B, lanes 5 and 6), suggesting that they are associated with chromatin and not a nuclear matrix structure. This simple protocol resulted in good biochemical fractionation and could be used with samples containing as few as 2 × 106 to 5 × 106 cells, making it suitable for cell cycle analysis.
Levels of hCdc6p fluctuate throughout the cell cycle.To analyze the relative levels of hCdc6p throughout the cell cycle, logarithmically growing Raji cells were separated according to cell size by centrifugal elutriation. The advantage of this synchronization procedure is that cells which have never been forced to leave the cell cycle are separated without the use of drugs that interfere with normal cell metabolism (reviewed in reference 30). The DNA content of each elutriated fraction was determined by staining with propidium iodide, and the percentage of cells in each phase of the cell cycle was calculated (Fig. 3A). Immunoblot analyses of whole-cell extracts (Fig. 3B) showed that the levels of hCdc6p are much lower in the first elutriated fractions (corresponding to small, early-G1 cells) than during the rest of the cell cycle. In contrast, levels of other proteins involved in initiation of replication, such as hOrc2p or hMcm proteins, did not fluctuate during the cell cycle. Very similar results have been obtained after centrifugal elutriation of 293 or Manca cells (not shown). These data suggest that the levels of hCdc6p fluctuate throughout the mammalian cell cycle, but in contrast to yeast Cdc6p, most hCdc6p is degraded as cells progress through mitosis.
Protein levels and chromatin association of hCdc6p, hOrc2p, and hMcm proteins during the cell cycle. An asynchronous culture of human Raji cells was subjected to centrifugal elutriation to isolate cells at different points of the cell cycle. The DNA content for the cells in each fraction is shown. (A) Percentage of cells in each phase of the cell cycle, estimated with the CellFIT computer program. (B) Levels of initiator factors in total cell extracts (TCE). Equivalent amounts of each total cell extract (normalized by cell number) were subjected to SDS-PAGE and transferred to nitrocellulose for immunobloting with the indicated antibodies. The concentration of cyclin A is also shown as a control of cell cycle progression. Lane A, asynchronous cells. (C) Cell cycle-regulated chromatin association of hOrc2p, hCdc6p, and hMcm proteins. Cells at different points in the cell cycle were subjected to the biochemical fractionation described for Fig. 2A. Immunoblots of the soluble protein fraction (S2) or chromatin-enriched fraction (P3) are shown.
Association of hOrc2p, hCdc6p, and hMcm proteins with chromatin across the cell cycle.An aliquot of each elutriated fraction was subjected to biochemical fractionation (Fig. 2A). The soluble protein fraction (S2) and chromatin-enriched fractions (P3) were tested for the presence of hOrc2p, hCdc6p, and hMcm proteins (Fig. 3C, left and right panels, respectively). The nucleoplasmic fractions (S3) contained very low amounts of protein and are not shown.
Virtually all hOrc2p was associated with chromatin throughout the entire cell cycle, the same as for its yeast counterpart. Interestingly, hCdc6p was also targeted to chromatin across the cell cycle. Soluble hCdc6p was also detected, but only during S phase and G2/M. This observation possibly reflects the fact that a fraction of hCdc6p is rapidly translocated to the cytosol after the G1/S transition, as has been proposed on the basis of immunostaining studies (26, 46, 52). The levels of soluble hCdc6p in this experiment seem higher than those detected in asynchronous cells. This can be explained because the later-elutriated fractions are enriched in late S and G2 cells, which contain soluble hCdc6p, whereas in the asynchronous population, most cells are in G1 or early S phase.
In contrast to the case for hOrc2p and hCdc6p, the association of hMcm3p, hMcm4p, and hMcm5p with chromatin was tightly regulated during the cell cycle. These proteins were detected on chromatin since early G1 and kept accumulating during G1 until the G1/S transition. All of them were then released as cells progressed through S phase. Some hMcm4p was detected in chromatin in the last elutriated fraction, suggesting that hMcm4p could reassociate with chromatin slightly before hMcm3p and hMcm5p, as has been reported recently for Xenopus (36). Very similar results were obtained with elutriated HeLa and Manca cells (not shown). These observations match other reports on the dynamics of chromatin association of hMcm proteins (11, 16, 21, 31, 51, 60) and further support the validity of the biochemical fractionation protocol that we used.
hCdc6p can be polyubiquitinated in vivo and is stabilized by the presence of proteasome inhibitors.Considering the cell cycle fluctuation of hCdc6p levels described above, we have analyzed the possibility that hCdc6p could be a substrate for ubiquitin-mediated proteolysis. The mitotic degradation of hCdc6p via the 25S proteasome would explain why the protein is more abundant in G2 cells than in early G1.
Two types of experiments were done to address this issue. First, it was determined whether the inhibition of the proteasome with specific inhibitors would increase the steady-state levels of hCdc6p in proliferating cells. Incubation of HeLa cells with a variety of proteasome inhibitors (LLnL, MG132, β-lactone, and PSI) increased the level of hCdc6p between two- and ninefold, whereas incubation with LLM, a calpain inhibitor, did not stabilize hCdc6p (Fig.4A). In contrast, inhibition of the proteasome did not affect hOrc2p levels. Stabilization of p53, a known substrate of ubiquitin-mediated proteolysis, was also observed as a positive control. As an additional negative control, the proteasome inhibitors did not affect the levels of TFIIB, a very stable transcription factor. Similar results were obtained with U2OS cells (not shown).
hCdc6p is targeted by ubiquitination for destruction by the proteasome. (A) Stabilization of hCdc6p by inhibitors of the proteasome. After treatment of HeLa cells with the indicated inhibitor, the cells were harvested and used to prepare total cell extracts. The steady-state levels of hCdc6p, as well as p53, increased in the presence of proteasome inhibitors but not in the presence of calpain inhibitor LLM. The levels of hOrc2p or TFIIB did not change significantly. (B) HeLa cells treated with dimethyl sulfoxide (control) or MG132 were subjected to the biochemical fractionation described for Fig. 2A. The levels of hCdc6p in the different fractions are shown. (C) In vivo polyubiquitination of hCdc6p. HeLa cells were transfected with plasmids expressing HA-tagged versions of hCdc6p, c-Myc, or TFIIB in the absence or in the presence of a plasmid that expresses His-Ubi. When indicated, 20 μM MG132 was added at 24 h posttransfection. Cells were harvested and lysed at 36 h after transfection. His-tagged proteins were purified and subjected to SDS-PAGE. After transfer to nitrocellulose, the samples were immunoblotted with anti-HA antibodies. Lanes 1 to 4 show 6% of the input protein.
To test whether a specific subpopulation of hCdc6p was stabilized, control or MG132-treated HeLa cells were subjected to biochemical fractionation. In HeLa cells, nucleoplasmic (S3) hCdc6p was slightly more abundant than in Raji cells (compare Fig. 2B and 4B). Upon treatment with MG132, both the chromatin-associated and the soluble hCdc6p were strongly stabilized (Fig. 4B), suggesting that both populations of hCdc6p can be degraded by the proteasome.
In a second approach, hCdc6p was found to be polyubiquitinated in vivo. HeLa cells were cotransfected with a plasmid expressing HA-tagged hCdc6p and a plasmid expressing His-Ubi. At 36 h after transfection, the cells were harvested and lysed, and the ubiquitinated proteins were purified from the total protein extract by affinity chromatography (see Materials and Methods). Ubiquitinated molecules were detected after SDS-PAGE by immunoblotting with an antibody directed to the HA epitope. A plasmid expressing HA-cMyc, a known substrate of the proteasome (53), was used as a positive control, and a plasmid expressing HA-TFIIB was used as a negative control.
Comparable levels of HA-hCdc6p, HA-cMyc, or HA-TFIIB were detected 36 h after transfection (Fig. 4C, lanes 1 to 3). When cells were cotransfected with plasmids expressing HA-hCdc6p and His-Ubi, several products with higher apparent masses than HA-hCdc6p were detected, corresponding to polyubiquitinated forms of hCdc6p (lane 7). These products were detected only in the presence of His-Ubi and the proteasome inhibitor MG132 (compare lanes 5 to 7). The requirement for MG132 may reflect the extremely short half-life of hCdc6p once it has been polyubiquitinated. Lanes 8 to 10 show the polyubiquitination of c-Myc, which can be detected even in the absence of MG132. On the other hand, TFIIB was not ubiquitinated under these conditions (lanes 11 to 13).
hMcm proteins are loaded onto chromatin during late mitosis.In the experiment shown in Fig. 3C, chromatin-bound hMcm proteins were detected even in early G1 cells, which is the stage at which hCdc6p is less abundant. The elutriation technique did not permit detailed investigation of the events that take place during mitosis and the M/G1 transition. To better define the hMcm loading step during this window of the cell cycle, a culture of HeLa cells was synchronized at prometaphase with nocodazole, and cells were collected at different time points after release from the block (Fig.5A). For each sample, the cellular DNA content was analyzed by flow cytometry after propidium iodide staining. Comparison of the DNA profiles corresponding to the asynchronous (lane A) and the nocodazole-arrested (lane N) cells shows that the mitotic block was complete. After the culture was reseeded in fresh medium, the cells progressed through mitosis and started entering G1 at around 100 min postrelease. The top immunoblot in Fig. 5A shows the mitotic destruction of cyclin B, the activator subunit of the Cdc2 kinase. Quantitation of this blot (Fig. 5B) revealed that the total levels of cyclin B dropped to below 50% after 80 min and to around 10% after 120 min without nocodazole.
Chromatin association of hMcm proteins at the M/G1 transition. (A) HeLa cells were synchronized at early mitosis with nocodazole (see Materials and Methods), and a fraction of cells was collected at different time points after release from the block. An aliquot of the cells isolated at each time point was used to determine the DNA content by flow cytometry (top panel), and the rest were subjected to the biochemical fractionation described for Fig. 4A. The presence of hCdc6p, hOrc2p, and hMcm proteins in the chromatin-enriched fraction (P3) was analyzed. The top panel shows the progressive degradation of cyclin B in total cell extracts (TCE) as the cells progress through mitosis. Lane A, asynchronous culture; lane N, nocodazole-arrested cells. (B) Quantitation of total cyclin B and chromatin-bound hMcm3p in the experiment shown in panel A. The results are expressed as percentages of the maximum signal in each curve.
The chromatin-nuclear matrix fraction was isolated, and the presence of hOrc2p, hCdc6p, and hMcm proteins was analyzed by immunoblotting. hOrc2p and hCdc6p were detected on chromatin in all samples. Virtually no hMcm3 protein was associated with chromatin in the nocodazole-arrested cells (Fig. 5A, lane N). However, at 100 min after release from the block, almost 100% of the Mcm3p subpopulation that associates with chromatin had been loaded (see also Fig. 5B). A sharp increase in other chromatin-bound MCM proteins, such as hMcm2p and hMcm5p, was detected around the same time with anti-pan-Mcm, an antibody that cross-reacts with different members of the MCM protein family. This result has two implications: (i) despite the mitotic destruction of the bulk of cellular hCdc6p, a fraction of it remained stable on chromatin during mitosis, and (ii) even though hOrc2p and hCdc6p are present on chromatin during early mitosis, the bulk of hMcm proteins were not loaded until a later stage, concomitant with the destruction of cyclin B.
We complemented this observation by examining the subcellular distribution of endogenous hMcm4p protein in HeLa cells by indirect immunofluorescence (Fig. 6). In most interphase cells, a uniform nuclear staining was observed, as has been reported for other hMcm proteins (55, 56). In prophase cells, however, when the nuclear envelope broke down, hMcm4p was uniformly distributed in the cell. Interestingly, during metaphase or anaphase, no hMcm4p staining could be detected on the condensed chromosomes, whereas in telophase, hMcm4p was transported back into the new nuclei. A similar result was observed with the pan-Mcm antibody (not shown). This result (combined with those of Fig.3 and 5) indicates that hMcm proteins start to be loaded on chromatin during late telophase and early G1, when the mitotic CDK activity was reduced by destruction of cyclin B but before cytokinesis was complete.
hMcm4p is loaded onto chromatin during late mitosis and early G1. The subcellular localization of hMcm4p was addressed by indirect immunofluorescence in HeLa cells. Cells were identified as interphasic or mitotic by direct observation of the chromatin condensation state, and the different stages of mitosis were determined by observation of the chromosome distribution. Representative photographs of each stage are shown. hMcm4p stains the nuclei of interphasic cells and the whole cell in prophase, metaphase, or anaphase. In the latter two phases, exclusion of staining in the chromatin is observed. In contrast, strong hMcm4p staining is detected in the chromatin of late telophase cells, which have not yet completed cytokinesis.
We have addressed the question of whether the model developed from studies with yeast to explain how replication origins are activated once and only once in each cell cycle is also valid for mammalian cells. According to this model, Cdc6p, Mcm proteins, and possibly other factors such as Cdt1 bind during early G1 to specific locations within the genome via interactions with the ORC. Several protein kinases, including CDKs and Dbf4p-Cdc7p, activate the pre-RCs, leading to entry into S phase. A good deal of evidence indicates that CDKs are also involved in the inhibition of rereplication during the G2 and M phases of the cell cycle, by exerting a negative control on the formation of functional pre-RCs (reviewed in reference25).
We have analyzed the dynamics of chromatin association of several initiator proteins across the mammalian cell cycle in rapidly proliferating cells. By synchronizing the cultures with centrifugal elutriation instead of drugs, interference with the cellular metabolism was minimized. Moreover, we used new antibodies that detect the endogenous levels of these proteins, thus avoiding possible artifacts derived from protein overexpression. Our data support the notion that, albeit with some significant differences, the mechanisms that control initiation of DNA replication in yeast are conserved in human cells. The finding that hOrc2p remains bound to chromatin throughout the entire cell cycle suggests that the potential for the ORC to serve as the DNA-bound landing pad for other initiation proteins is conserved. Formation of pre-RCs, if they can be defined by the loading of hMcm proteins onto chromatin, starts at late mitosis and continues during early G1, a window of opportunity during which the CDKs are inactive (48). Our data show that hMcm proteins do not bind to chromatin even in the presence of chromatin-bound hOrc2p and hCdc6p, until cyclin B is degraded and therefore the mitotic kinase activity is reduced. From this moment on, hMcm proteins are present on chromatin until the onset of S phase and are progressively released afterwards. This alternation of hMcm proteins between soluble and chromatin-bound states is consistent with previous immunostaining and biochemical data obtained with several hMcm protein subunits (16, 21, 31, 51,60). Our results also parallel the recent finding that the Chinese hamster Mcm2p starts to bind to both early- and late-replicating chromatin in late telophase, before the so-called origin decision point (66), and keeps accumulating during G1 in CHO-400 cells (11). Therefore, the timing of pre-RC formation is almost identical in different mammalian systems.
In contrast to the case for the ORC and the MCM proteins, the regulation of Cdc6p across the cell cycle is very different in proliferating yeast and mammalian cells. In both budding and fission yeasts, Cdc6 and Cdc18 proteins are degraded shortly after the G1/S transition. Expression of hCdc6 gene is E2F regulated (67), and the levels of hCdc6 mRNA peak at the G1/S transition (65). HCdc6p levels, however, are fairly constant in the cell cycle, with the exception of early G1 cells, in which the hCdc6p concentration is much lower. Therefore, most hCdc6p might be degraded during mitosis. We found that hCdc6p can indeed be polyubiquitinated in vivo and that inhibition of the proteasome results in increased steady-state levels of hCdc6p. These data combined indicate that hCdc6p is a bona fide substrate for ubiquitin-mediated proteolysis. The region of hCdc6p that targets it for degradation by the proteasome has been recently mapped to the N terminus of the protein (B. O. Petersen, C. Wagener, M. Melixetian, F. Marinori, E. L. Denchi, C. Matteucci, and K. Helin, personal communication).
Our study also addresses the issue of hCdc6p subcellular localization. Some groups have concluded that the protein is mostly nuclear in G1 but is translocated to the cytosol after the G1/S transition (26, 46, 52). This conclusion is mostly supported by experiments in which epitope-tagged versions of hCdc6p were introduced in human cells and the overexpressed proteins were detected by indirect immunofluorescence. The nuclear-cytoplasmic translocation seems to correlate with the phosphorylation of hCdc6p by cyclin A-Cdk2 (26, 46) and depends on exportin-1 (26). On the other hand, other reports indicate that endogenous hCdc6p is mostly nuclear (64, 65) and is actually associated with a nuclear structure (17).
Our present analysis with elutriated Raji cells revealed that a significant fraction of endogenous hCdc6p remained associated with chromatin from G1 until mitosis. It should be noted that this is compatible with the previously published observations, because the immunostaining technique cannot rule out the possibility that some hCdc6p remains in the nuclei during S and G2. Some soluble hCdc6p was detected after cells enter S phase, in agreement with the translocation model. Coverley et al. (8) recently reported a series of experiments performed with a cell-free system in which DNA replication was induced in isolated 3T3 cell nuclei by incubation with cytosolic extracts from the same cells in the presence of defined amounts of exogenous XCdc6p. The main conclusion from this study was that chromatin-bound Cdc6p persists in S and G2, while soluble Cdc6p is destroyed in a cyclin A-Cdk2 dependent process. This report is consistent with our own findings with endogenous hCdc6p and indicates that proteolysis of hCdc6p is likely another regulatory step in the mammalian cell cycle.
A model for hCdc6p regulation during the cell cycle that integrates the new data with all the previous observations is presented in Fig.7. In late mitosis and early G1, chromatin-bound hORC and hCdc6p are required to load the hMcm proteins onto chromatin, forming the pre-RCs at origins of replication. Later, just before the origins fire, other factors are loaded to form the preinitiation complex. One of these factors is hCdc45p, a protein that in turn could recruit replisome proteins to the origins, as happens in yeast (69). When cyclin E-Cdk2 triggers S phase and cyclin A-Cdk2 becomes active, most hCdc6p is phosphorylated and detached from chromatin, and some is translocated to the cytosol. However, a fraction of hCdc6p remains associated with chromatin, perhaps because it is not accessible to the CDKs. A specific cellular mechanism might contribute to keep a fraction of hypophosphorylated hCdc6p, because hCdc6p has been found to associate with a novel regulatory subunit of protein phosphatase 2A (68). Nucleoplasmic and cytosolic hCdc6p are degraded by ubiquitin-mediated proteolysis.
A model for the regulation of hCdc6p in the cell cycle. See the text for details. IC, initiation complex.
Whether there is a role for cytosolic hCdc6p remains unknown, but it could send a signal that prevents mitosis until it is degraded by the 26S proteasome. The possibility of Cdc6p coordinating DNA replication and mitosis has been previously suggested for yeast (4, 28,47), although the molecular mechanism of this mitotic inhibition has not been elucidated.
In our model, the post-RC in human cells would contain the hORC as well as hCdc6p. But interestingly, this chromatin-bound pool of hCdc6p did not recruit hMcm proteins onto chromatin during G2 phase, or during mitosis until cyclin B was degraded. Therefore, the negative control exerted by active CDKs on the formation of pre-RCs seems to be conserved. Consistent with this idea, inhibition of the CDKs permitted the MCM proteins to reload onto chromatin when mammalian G2nuclei were incubated in S-phase Xenopus extracts (7). It is now clear that multiple mechanisms coexist to ensure the inhibition of pre-RC formation after S phase. These additional mechanisms likely include the regulated instability of the largest subunit of the hORC (41; H. Zou-Yang, H., J. Méndez, M. Hidaka, and B. Stillman, unpublished data) and geminin, a protein first identified in Xenopus that is destroyed as cells progress through mitosis (38).
What is the execution point of human Cdc6p?Based on the evidence accumulated with yeast and Xenopus, it seems reasonable to assume that hCdc6p will be responsible for the recruitment of hMcm proteins onto ORC-bound chromatin at the M/G1 transition. However, if the sole function of hCdc6p was to load Mcm proteins onto pre-RCs during the M/G1transition, it would be difficult to explain why phosphorylation of hCdc6p by cyclin A-Cdk2 at the G1/S transition seems to be essential for entry into S phase (26). This conclusion is based on the observation that overexpression of a nonphosphorylatable form of hCdc6p (bearing the triple mutation S54A/S74A/S114A) inhibits cellular DNA replication. This result is different from that of Petersen et al. (46), where no significant defect in DNA replication was observed after transfection or microinjection of the same hCdc6p mutant derivative. Interestingly, microinjection of mutant versions of hCdc6p which impair ATP binding and/or hydrolysis also results in G1/S arrest (20), reinforcing the hypothesis that hCdc6p has a function at the G1/S-phase transition. One simple explanation that we favor is that hCdc6p is required to load MCM proteins onto chromatin in late G1phase, particularly in cells that are entering into the proliferation cycle from G0 phase or in cells that have an extended G1 phase. This situation would resemble that in S. cerevisiae cells undergoing a prolonged G1 phase (e.g., after treatment with α-factor), where there is a new peak of Cdc6p expression just before the G1/S transition. Therefore, we propose that hCdc6p is essential and rate limiting for initiation of DNA replication, as has been observed (35, 58)
Is there another function for the chromatin-bound hCdc6p during S phase and G2? Very recent data obtained with yeast indicate that Cdc6p cooperates with the ORC to determine origin specificity, in an ATP-dependent manner (39). It is tempting to speculate that the chromatin-bound form of hCdc6p could cooperate with the hORC to bind to specific replicator sequences. As the nature of such sequences in higher eukaryotes remains unknown, this may be an exciting topic for future research.
We thank W. Tansey for his help with the proteasome inhibition and in vivo ubiquitination experiments, R. S. Williams for providing purified recombinant hCdc6p, C. Bautista for her work at the Cold Spring Harbor Laboratory monoclonal antibody facility, K. Brown for her help with epitope mapping of the anti-hCdc6p antibodies, A. Koff for his help with the elutriation protocol, N. Hernandez for anti-TFIIB antibodies, D. Bohmann for plasmid pMT107, A. Verreault for his suggestions on the fractionation protocol, K. Cronin for excellent technical assistance, and A. Losada and J. Chong for useful comments on the manuscript.
This work was supported by the National Cancer Institute (grant CA13106). J.M. was the recipient of postdoctoral fellowships from Fundación Ramón Areces (Spain) and the Human Frontier Science Program Organization.
Received 7 July 2000.
Returned for modification 2 August 2000.
Accepted 15 August 2000.
↵* Corresponding author. Mailing address: Cold Spring Harbor Laboratory, P.O. Box 100, 1 Bungtown Rd., Cold Spring Harbor, NY 11724. Phone: (516) 367 8384. Fax: (516) 367 8879. E-mail:stillman{at}cshl.org.
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Matthew Buzzi Razer Blade Stealth (2019) Razer's 2019 Blade Stealth ultraportable, a smart refinement of its excellent predecessor, packs a sharp (and sharp-edged) redesign, a new dedicated graphics option, and snappy performance with a strong supporting feature set.
After fully recharging the laptop, we set up the machine in power-save mode (as opposed to balanced or high-performance mode) and make a few other battery-conserving tweaks in preparation for our unplugged video rundown test. (We also turn Wi-Fi off, putting the laptop in Airplane mode.) In this test, we loop a video—a locally stored 720p file—with screen brightness set at 50 percent and volume at 100 percent until the system conks out.


text:
Rufflets Country House Hotel
Rufflets Country House Hotel in St Andrews, is one of the oldest & best country house hotels in Scotland. This idyllic turreted mansion house, set on 10 acres of award-winning gardens, has been in the same family ownership since 1952. With an enviable location on the outskirts of St Andrews, you can enjoy the tranquility of the Fife countryside with the bustle of the town only 1.5 miles away. Rufflets offers a wide range of accommodation options, with 24 individually designed bedrooms. The elegant and spacious Terrace Restaurant is perfect for a romantic dinner for two or for a larger family gathering, and the hotel's own garden supplies much of the fresh produce used in the seasonally changing menus. Golf, of course, is the main attraction in the area. St Andrews may be the acclaimed 'Home of Golf' but the medieval town also offers excellent shopping, art galleries, restaurants and museums. Regarded as one of finest hotels in St Andrews, Rufflets has been awarded 4 Red Stars by the Automobile Association and 4 Gold Stars by Visit Scotland.
Rufflets offers 24 individually-styled and tastefully decorated bedrooms. Unlike most of the larger chain hotels, rooms are different shapes and sizes, which adds to their individual character and distinct charm. Please note that the bedrooms in the main house are located on the first and second floors of the hotel and are accessed by stairs.
All Guestrooms Feature:
Iron & Trouser Press
Molton Brown Toiletries
Terrace Restaurant:
Decorated with light faux wood paneling, the deep terracotta walls are enhanced by a collection of striking still life prints featuring the work of the Scottish Colorists, Cadell and Peploe.
The spacious restaurant has an unfussy, classic contemporary feel to it. Tables, clothed in crisp white linen, are arranged to take full advantage of the space and the views of the formal terraced garden. The fully upholstered, high backed chairs are designed for comfort. In the evening, sparkling glass, candlelight, fine china and floodlit gardens set the scene for a relaxed dining experience, whether it is for a romantic dinner for two or a larger group.
The 2AA Rosette Restaurant, which features in all the major guides, offers a daily changing, fixed price menu with ingredients sourced locally, some even grown in our own gardens.
The City of St. Andrews
On The Map: St Andrews is located on Scotland's east coast. Dundee is less than 30 minutes to the north, while Edinburgh 1.5 hours to the south.
Poised and well-groomed, St. Andrews is the pilgrimage site for golfers around the world. It is also Scotland's oldest university town, founded in 1410. The town of St. Andrews is small—only three main streets and an open, airy feel with long stretches of sandy beach on either side of town. There are acres-plus of golf links in every direction. The locals are proud of their town and it has a refined, old-fashioned ambience. Many original buildings have survived, and the castle and cathedral have been rebuilt to preserve their remains. The main streets and cobbled alleys are lined with crooked houses. Medieval churches line up and meet at the ruin of the 12th century cathedral. St. Andrews is a light, seaside resort that feels busier and larger than it is. More importantly, it is one of the most notable and historic towns in Scotland.


text:
Gurley over Gronkowski in Madden NFL 19 video game matchup
Posted: Jan 30, 2019 / 03:40 PM CST / Updated: Jan 30, 2019 / 03:40 PM CST
ATLANTA (AP) — It might have zero bearing on Sunday's Super Bowl, in which the Patriots are a 2½-point favorite over the Rams. Still, on Tuesday night, Los Angeles All-Pro running back Todd Gurley had the upper hand against New England star tight end Rob Gronkowski.
Gurley beat Gronkowski 21-6 in a Madden NFL 19 Super Bowl 53 preview as part of Xbox Sessions: Game Before The Game. Some interceptions and a missed extra point did in Gronkowski in the contest.
"Safe to say that game went good, I got to meet Gronk and hang out with him playing the game," said Gurley, who led real football in scoring this season with 21 touchdowns. "Honestly I had one of my best Madden games I've ever had. I don't know how I did it, but (Rams quarterback Jared) Goff came through in the clutch for me. It was a great time."
Not so much for Gronkowski, who wasn't exactly Tom Brady at the controls in the video version of the Super Bowl.
"It didn't go so well for me there," he said. "You know, I threw a couple of interceptions, I didn't make enough plays; 21-6 I lost.
"I got to come out better on Sunday for sure."
Gurley and Gronk appeared at the VIP Microsoft Lounge, where they were cheered on by some wildly enthusiastic fans. The good-natured but intense battle isn't likely to have a similar final score given the powerful offenses on both sides, and how NFL teams have lit up the scoreboard all season.
"We feel great about it because we're keeping the same mentality we have every game," said Gronkowski, whose Patriots are in their third straight Super Bowl and seek their sixth title. "It's about us doing our job, as coach says, and that's what we're prepared to do."
For Super Bowl newcomer Gurley — the Rams last made the game in 2000 when they represented St. Louis — this isn't just a chance for a championship. It's a homecoming.
"This is exciting, we're out here in ATL for Super Bowl 53 playing against one of the greatest quarterbacks of all time," Gurley said. "It's exciting to be back in Georgia where I went to school.
"They're a great team and we have a great team so looking forward to matching up against them."
Particularly if his Rams are as successful as Gurley was against Gronk.
More AP NFL coverage: https://apnews.com/NFL and https://twitter.com/AP_NFL
More The Big Game Stories
At the Super Bowl, eyes on iPhones not the field
by Ashley Bridges / Feb 4, 2019
ATLANTA (NEXSTAR) --
Millions of eyes were glued to the TV last night for the Super Bowl.
Renewed halftime show controversy: wardrobe malfunction vs inconsistent rules?
by James Clark / Feb 4, 2019
Social media and even a few news media outlets are exploring the question: why can Adam Levine of Maroon 5 go shirtless at a Super Bowl halftime show but Janet Jackson's wardrobe malfunction was highly controversial?
Blue-checked Twitter members like Katie Nolan, Aisha Tyler, Aminé, are among those asking the question on social media. Nolan's comment, "Super Bowl halftime nipple rules feel inconsistent," posted Sunday evening, had more than 27,000 people talking as of Monday, according to Twitter.
Patriots defeat Rams in Super Bowl LIII, 13-3
by The Associated Press / Feb 4, 2019
ATLANTA (AP) — Defenses dominated what was supposed to be a super shootout, until Tom Brady led one classic drive to win the New England Patriots their record-tying sixth Super Bowl.
Brady threw two perfect passes to Rob Gronkowski to set up rookie Sony Michel's 2-yard score — the only touchdown in the lowest-scoring Super Bowl ever. That put New England up 10-3. A late field goal clinched the game 13-3.
Top Stories / 1 min ago


text:
Q: Restart powershell script My script counts the audio files on the PC and checks if the drive entered in the input is the system drive. If it is, a message is sent to Return or Exit the script. I created the :INI and :EXIT paragraphs, but the script does not return to :INI?
Script:
clear-Host
:INI
cls
Write-Host "                                          Count Audio Files on PC" -ForegroundColor Yellow
$br
$local= Read-Host -Prompt " Enter your path Music folder. Eg. D:\*"
$location = "$local"

$locTst = $location.substring(0,2)

# Check if the drive c:
If ($locTst -eq "c:") {
    write-host -ForegroundColor Red " Drive $locTst does not allow access to external scripts!"
    $dirLoc= Read-Host -Prompt " Return?(Y/N)"
    if ($dirLoc -eq "y") {
       :INI
    } else {
       :EXIT
    }
}

commands to counting audio files...

:EXIT
exit

How to make the script return to :INI to restart the script and also in :EXIT the powershell screen is closed?
I tried to use the commands from this link, but they give an error in Powershell ISE and I couldn't debug the code.
how can i allow y/n to be input

A: In keeping with your current PowerShell coding constructs, give this slight modification a try:
clear-Host
while ($true) {
    cls
    Write-Host "                                          Count Audio Files on PC" -ForegroundColor Yellow
    $br
    $local= Read-Host -Prompt " Enter your path Music folder. Eg. D:\*"
    $location = "$local"

    $locTst = $location.substring(0,2)

    # Check if the drive c:
    If ($locTst -eq "c:") {
         write-host -ForegroundColor Red " Drive $locTst does not allow access to external scripts!"
         $dirLoc= Read-Host -Prompt " Return?(Y/N)"
         if ($dirLoc -eq "y") {
             continue
         } else {
             break
         }
    }

    # commands to counting audio files...
    break
}

exit

This uses a while loop to continue keeping users inside your INI section, so long as they respond with "y" for drive C: content; and will break out of the loop (and exit) if not.


text:
Q: Math pow alternative in java I have a need to sum up power of 2 from any digit x to 0.
If x=6,desired sum is 2pow6+2pow5+.....1.
While I can always write a algorithm to wind down to 0 using Math.pow - this function seems notorious performance-wise in a loop.
Would appreciate if someone could help achieving the same using shift binary operators - I hear they are much more efficient than pow.

A: You don't have to calculate it in a loop, what you are trying to compute is equivalent to
Math.pow(2, x+1) - 1

Even better, you can calculate it like torquestomp suggested, which will be faster:
(1 << (x + 1)) - 1

A: 2^n + 2^(n-1) + 2^(n-2) + ... + 2 + 1 = (2^(n+1) - 1) = ((1 << (n+1)) - 1)


text:
Harleston Funeral Home records
Avery Research Center for African American History and Culture
The Harleston Funeral Home Papers consist of 17 volumes which document the deceased, customers, and funeral expenses. They are organized into three series. The Funeral Records (1910-1923; 1929-1950; 1953-1958) are chronologically organized account books detailing funeral and related expenses. All of them contain indexes; some indexes list the deceased while others list the person paying for the funeral. Extensive biographical statistics are often given, and there are various mentions of other businesses using hearses such as those run by N. C. Boags, R. M. Snype and others and references to the Mickey Brothers and Eugene Gadsen (or Gadsden) funeral homes. The Insurance Records (1951-1970) also list deceased and related expenses, but include individual insurance information, and omit extensive biographical information. The Financial Records (1905-1907; 1951-1957) consist of an inventory, receipt, and account book, which lacks individual funeral records and statistics, but detail business expenses with hundreds of decorative bill heads. Researchers are required to use microfilmed records.
Collection Arrangement
1. Funeral Records
2. Insurance Records
3. Financial Records
Harleston Funeral Home (Charleston, S.C.) (Organization)
Material is in English
Papers have been microfilmed. Researchers are required to use microfilm.
Box 1 - See Reel 1
The nature of the Avery Research Center's archival holdings means that copyright or other information about restrictions may be difficult or even impossible to determine despite reasonable efforts. The Avery Research Center claims only physical ownership of most archival materials.
The materials from our collections are made available for use in research, teaching, and private study, pursuant to U.S. copyright law. The user must assume full responsibility for any use of the materials, including but not limited to, infringement of copyright and publication rights of reproduced materials. Any materials used for academic research or otherwise should be fully credited with the source.
The original Harleston Funeral Home was established in Charleston, South Carolina in 1901 by Edwin G. Captain Harleston (1854-1931) and his brother, Robert Harleston (1846-1915), under the name Harleston Brothers Funeral Home. It was one of the first funeral homes for African Americans in Charleston. The same year that it was founded, it merged with Mickey Funeral Home following the death of its proprietor, Edward H. Mickey (1850-1899). Mickey had married E.G. Harleston's sister, Hannah Harleston Mickey (1848-1928). As a widow, she joined with her brother E.G. Harleston as joint proprietors of the business and in 1903, the name was changed to Mickey and Harleston Funeral Home. In 1913 the firm was dissolved and E.G. Harleston established a separate funeral operation by erecting a building at 121 Calhoun Street. The business was thereafter known as Harleston Funeral Home. That same year, E.G. Harleston enlisted his artist son, Edwin A. Harleston (1882-1931) to help with the business. They ran the funeral home together until 1931, when they both died. The business then stayed in the Harleston family until 1961, when Jobe Colbert bought controlling interest. In 1978, Grippon A. Boags purchased the business and renamed it Harleston-Boags Funeral Home. It still (2006) operates at 121 Calhoun Street.
3.5 linear feet (7 archival boxes)
The original Harleston Funeral Home was established in Charleston, South Carolina in 1901 by Edwin G. Captain Harleston (1854-1931) and his brother, Robert Harleston (1846-1915), under the name Harleston Brothers Funeral Home. It was one of the first funeral homes for African Americans in Charleston. The Harleston Funeral Home Papers consist of 17 volumes which document the deceased, customers, and funeral expenses. They are organized into three series. The Funeral Records (1910-1923; 1929-1950; 1953-1958) are chronologically organized account books detailing funeral and related expenses. All of them contain indexes; some indexes list the deceased while others list the person paying for the funeral. Extensive biographical statistics are often given, and there are various mentions of other businesses using hearses such as those run by N. C. Boags, R. M. Snype and others and references to the Mickey Brothers and Eugene Gadsen (or Gadsden) funeral homes. The Insurance Records (1951-1970) also list deceased and related expenses, but include individual insurance information, and omit extensive biographical information. The Financial Records (1905-1907; 1951-1957) consist of an inventory, receipt, and account book, which lacks individual funeral records and statistics, but detail business expenses with hundreds of decorative bill heads.
Acquisitions Information
Donated by Grippon A. Boags, July 1996
Processed by Jessica Lancia, 2006
Encoded by Melissa Bronheim, July 2010
Edited by Aaron Spelbring, January 2014
Funding from the Gaylord and Dorothy Donnelley Foundation supported the processing of this collection.
Funding from the Council on Library and Information Resources supported the collection processing and encoding of this finding aid.
African American businesspeople -- South Carolina -- Charleston
African Americans -- South Carolina -- Charleston -- Statistics, Vital
Eugene Gadsden Funeral Home (Charleston, S.C.)
Funeral homes -- South Carolina -- Charleston
Funeral rites and ceremonies -- South Carolina -- Charleston
Harleston family
Harleston, Edwin Augustus, 1882-1931
Harleston, Edwin Gailliard, 1854-1931
Harleston, Robert, 1846-1915
Mickey Funeral Home (Charleston, S.C.)
Mickey family
Mickey, Hannah Harleston, 1848-1928
Undertakers and undertaking -- Equipment and supplies
Undertakers and undertaking -- South Carolina -- Charleston
Undertakers and undertaking -- United States
Boags, Grippon A. (Donor, Person)
Inventory of the Harleston Funeral Home records, 1900 - 1976
Processed by: Jessica Lancia; machine-readable finding aid created by: Melissa Bronheim
Language of description note
Description is in English
Funding from the Gaylord and Dorothy Donnelley Foundation and the Council on Library and Information Resources supported the collection processing and encoding of this finding aid.
Part of the Avery Research Center for African American History and Culture Repository
http://avery.cofc.edu/
Charleston South Carolina 29424 United States
averyresearchcenter@cofc.edu
[Identification of item], Harleston Funeral Home Papers, Avery Research Center, College of Charleston, Charleston, SC, USA.
[Identification of item], Harleston Funeral Home Papers, Avery Research Center, College of Charleston, Charleston, SC, USA. https://findingaids.library.cofc.edu/repositories/3/resources/278 Accessed January 21, 2022.


This comforting vegetable beef stew is perfect for cold winter evenings, and filled with earth vegetables for added nutrition.
Be sure to pick out a lean cut of beef, like the eye of round noted, and look for a brand that is free of antibiotics and hormones, like Laura's Lean Beef. Note that "Lean" beef contains less than 10g of fat, 4.5g or less of saturated fat, and less than 95mg of cholesterol per serving, by USDA standards. "Extra lean" contains less than 5g of fat, 2g of saturated fat and 95mg of cholesterol per serving.
This vegetable beef stew is naturally dairy-free / non-dairy, egg-free, nut-free, peanut-free, and gluten-free (but stick with the wheat-free tamari – and yes, Worcestershire is usually gluten-free!).
For soy-free, you can try coconut aminos as a soy sauce substitute, or simply up the Worcestershire a touch and season to taste with additional salt, if needed.
Heat the oil in a large skillet over medium-high heat. Sear the meat cubes until browned on all sides.
In a separate bowl, combine the canned tomatoes, tomato sauce, soy sauce, Worcestershire sauce, beef stock, basil, salt and pepper. Mix thoroughly.
Place the meat and vegetables in a roasting pan, and pour the tomato mixture over top.
Cover and bake for 45 minutes. Reduce the heat to 250°F and bake for 2 hours or until meat is very tender to the fork. Baste occasionally.


I got a big commission for an illustration project. I have until September to finish it: The commission is to create (lots of!) illustrations for an educational website. They should be simple, fun, and able to show different characters in a standard style.
I have done some concept art on the project and think that I am finding a style to work with. I was partly inspired by the animations on the educational Youtube Channel "Crash Course". But the client wanted more realistic proportions and more expessive faces. So, that's how you go: you start with some inspiration and work with the client and your own ideas to find out what works. And this takes time. A friend of mine was quite surprised to hear that a full comission can easily take 100h of work! Processes don't just happen. But if you stay comitted to them, they develop to a place you didn't anticipate from the start. And most of the time, that's a good thing.


Right now they are my favorite. I literally wear them just about every day. I am not sure how much longer they will last but if they tarnish tomorrow I will have gotten my money's worth. I made the mistake of spraying my perfume on one of them and I see a few tiny spots on that one. I probably have a few months left just remember not to get perfume on them and they will probably last a very long time..
I love these earrings! They're so pretty and I wear them often.
You had very few choices in white gold.
i love white gold and sterling silver and was impressed with the price and design.
I thought that the earrings were very cute!!!!
I have looked for these earrings everywhere and when I found them here, I had to get them. I am so happy with everything that I have purchased here, I love shopping here. Great items at a great price. It's a protected site, purchase guarantee, and best prices anywhere.
Very pretty. Just the right size.
i liked the ear rings.
They are white gold, unique, pretty and well priced!
Looking for a pair to replace the one earring I lost.
I fell in love with these hoops when I first saw them! It took me several days but I finally made my decision! Hope my choice turns out to be the right one!
I can only wear silver or gold. I also find drop earrings or hoops more comfortable than posts. And I like the bamboo look.
They are a nice wearable size for most people and I do like the bamboo styling.
I think it is excellent for the price.
I need a pair of silver/white gold earrings. I am tall. the size attracted me. A steal of a price.
these earrings are beautiful and the price was unbeliveable.
Because they are beautiful and different!
I lost my wedding ring before my husband died and it was yellow gold bamboo. I love bamboo jewelry.
looks good when paired with yellow gold. just hope they are a medium size earring.
Like hoop earrings and the price.
What are the dimensions of the earrings?
BEST ANSWER: Earrings dimensions: 1.5"(L) x 1.5"(W).
what are the measurement of these hoops ?
Are these solid white gold or not? I'm seeing the title, but when questioned, it states they are really brass. Which is correct?
BEST ANSWER: They are white gold-plated brass.
BEST ANSWER: White gold-plated brass.