text:
Next Computing & The Internet
Next Web & Digital Design Applications
Next Computer Games Design
Computer Games Design 9780691196381-01-000 9780691196381-01-000 9780691196381
https://www.whsmith.co.uk/products/powerup-unlocking-the-hidden-mathematics-in-video-games/matthew-lane/paperback/9780691196381-01-000.html
Power-Up Unlocking the Hidden Mathematics in Video Games
By Matthew Lane (Author)
https://www.whsmith.co.uk/products/powerup-unlocking-the-hidden-mathematics-in-video-games/matthew-lane/paperback/9780691196381-01-000.html £11.19 rrp £15.99 Save £4.80 (30%)
A fun and lively look at the mathematical ideas concealed in video games Did you know that every time you pick up the controller to your PlayStation or Xbox, you are entering a world steeped in mathematics? Matthew Lane reveals the hidden mathematics in many of today's most popular video games-and explains why mathematical learning doesn't just happen in the classroom. He discusses how gamers are engaging with the traveling salesman problem when they play Assassin's Creed, why it is mathematically impossible for Mario to jump through the Mushroom Kingdom in Super Mario Bros., how The Sims teaches us the mathematical costs of relationships, and more. Power-Up shows how the world of video games is an unexpectedly rich medium for learning about the mathematical ideas that touch our lives-including our virtual ones. 130 color illus.
Matthew Lane is a mathematician and cofounder of Rithm School, where he works with aspiring software engineers. He writes about the intersection of mathematics and popular culture at mattlane.us.
Contributor: Matthew Lane
Imprint: Princeton University Press
Biography: Matthew Lane is a mathematician and cofounder of Rithm School, where he works with aspiring software engineers. He writes about the intersection of mathematics and popular culture at mattlane.us.
Matthew Lane
https://www.whsmith.co.uk/products/powerup-unlocking-the-hidden-mathematics-in-video-games/matthew-lane/paperback/9780691196381.html £11.19 rrp £15.99 Save £4.80 (30%)


text:
Q: Reactive Extension key press media controls I have a media application that allows the user to Play, Pause, step frame by frame, FastForward, etc.  I am attempting to use Rx to get the following behavior for stepping and FastForward.

*

*If the user clicks the right arrow less than 2 times/300ms I want to frame step.

*If the user holds down the right arrow I want to fast forward until the right arrow button is released.

I think I have the fast forward part correct, but am not sure how to craft this to get the step functionality. I am also open to "better" ways to do the fast forward.
//start FF when we get 2 key presses within the threshold time
Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown")
            .Where(k => k.EventArgs.Key == Key.Right)
            .Timestamp()
            .Buffer(2)
            .Where(x => (x[1].Timestamp - x[0].Timestamp).Milliseconds < 300)
            .Subscribe(x =>
                {
                    Console.WriteLine("FastForward GO");
                    _viewModel.FastForward();
                });

//stop ff on the key up
Observable.FromEventPattern<KeyEventArgs>(this, "KeyUp")
            .Where(k => k.EventArgs.Key == Key.Right)
            .Subscribe(x => {
                Console.WriteLine("FastForward STOP");
                _viewModel.StopFastForward();
            });

A: Solution
var up   = Observable.FromEventPattern<KeyEventArgs>(this, "KeyUp")
                     .Where(x => x.EventArgs.KeyCode == Keys.Right);

// Take, Concat, and Repeat work together to prevent repeated KeyDown events.
var down = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown")
                     .Where(x => x.EventArgs.KeyCode == Keys.Right)
                     .Take(1)
                     .Concat(up.Take(1).IgnoreElements())
                     .Repeat();

var t = TimeSpan.FromMilliseconds(300);

var tap = down.SelectMany(x =>
    Observable.Amb(
        Observable.Empty<EventPattern<KeyEventArgs>>().Delay(t),
        up.Take(1)
    ))
    .Publish()
    .RefCount();

var longPress = down.SelectMany(x =>
    Observable.Return(x).Delay(t).TakeUntil(tap)
    );

There's multiple ways to do this, but this works at getting the "longPress" you need, as well as the "tap". You can use longPress to start fast-fowarding, up to stop fast-forwarding, and tap for frame-stepping.
tap yields when a key has been pressed and released within a timespan of t.
longPress yields when the key has been held down for longer than t.
up yields when the key has been released.
Explaination
A problem exists because the KeyDown event is repeated multiple times for each physical press of a key.
var down = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown");

In this case, we need a way to filter out the repeated KeyDown events. We can do that by using a combination of operators. First, we'll use Take(1). This will yield the first event and ignore the rest.
var first = down.Take(1);

If we only needed to get a single actual key press, this would be great. But, alas, we need to get all of the actual key presses. We need to wait for the KeyUp event to occur and start the whole thing over. To do this, we can use a combination of Concat and Repeat. For the concat observable, we need to make sure we're only taking 1 up event, and that we're ignore the elements of the up observable, otherwise we end up feeding all of the up events into our new observable.
var down = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown")
                     .Take(1)
                     .Contact(up.Take(1).IgnoreElements())
                     .Repeat();

This gives us the actual down events, without the in-between repeated events.
Now that we've cleaned up our source observables, we can start composing them in useful ways. What we're looking for is a "tap" event and a "long press" event. To get the tap event, we need to take a single actual down event, and make sure that it isn't held down too long... One way to do this is using the Amb operator.
var tap = down.SelectMany(x =>
    Observable.Amb(
        Observable.Empty<EventPattern<KeyEventArgs>>().Delay(t),
        up.Take(1)
    ))

The Amb operator stands for "ambiguous". It takes a number of Observables, listens to each one, and waits for them to yield something. Once one of them yields an event, the Amb operator ignores (disposes the subscriptions of) the other observables.
In our case, for each down event that occurs, we use the SelectMany and Amb operator to check to see which yields or completes first... a single up event, or an empty observable that completes after a timespan of t. If the up event occurs before the the empty observable completes, its a tap. Otherwise, we ignore it.
Now we can do a similar thing for "long press", except this time we want to delay the KeyDown event until we know that it's not a tap. We can use a combination of the Delay and TakeUntil operators to do this. Delay makes sure the long press doesn't occur before a tap can be registered, and TakeUntil makes sure we ignore the KeyPress if it turned out to be a tap after all.
var longPress = down.SelectMany(x =>
    Observable.Return(x).Delay(t).TakeUntil(tap)
    );

Generalized Solution
This version works for any key.
var up = Observable.FromEventPattern<KeyEventArgs>(this, "KeyUp");
var downWithRepeats = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown");

var down =
    Observable.Merge(
        up.Select(x => new { e = x, type = "KeyUp" }),
        downWithRepeats.Select(x => new { e = x, type = "KeyDown" })
    )
    .GroupByUntil(
        x => x.e.EventArgs.KeyCode,
        g => g.Where(y => y.type == "KeyUp")
    )
    .SelectMany(x => x.FirstAsync())
    .Select(x => x.e);

var t = TimeSpan.FromMilliseconds(300);

var tap = down.SelectMany(x =>
    Observable.Amb(
        Observable.Empty<EventPattern<KeyEventArgs>>().Delay(t),
        up.Where(y => y.EventArgs.KeyCode == x.EventArgs.KeyCode).Take(1)
    ))
    .Publish()
    .RefCount();

var longPress = down.SelectMany(x =>
    Observable.Return(x).Delay(t).TakeUntil(
        tap.Where(y => y.EventArgs.KeyCode == x.EventArgs.KeyCode)
        )
    );

Usage
Observable.Merge(
    down     .Select(x => string.Format("{0} - press",      x.EventArgs.KeyCode)),
    tap      .Select(x => string.Format("{0} - tap",        x.EventArgs.KeyCode)),
    longPress.Select(x => string.Format("{0} - longPress",  x.EventArgs.KeyCode)),
    up       .Select(x => string.Format("{0} - up",         x.EventArgs.KeyCode))
)
.ObserveOn(SynchronizationContext.Current)
.Select(x => string.Format("{0} - {1}", x, DateTime.Now.ToLongTimeString()))
.Subscribe(text => this.myTextBox.Text = text);

A: Here's an alternative to Chris's that gives three streams, one for clicks, one for begin holds and one for end holds. Makes use of TimeInterval for recording duration between events.
WinForms Version
We can capture KeyDown eliminating repeats by using GroupByUntil to group KeyDown until a KeyUp occurs:
TimeSpan limit = TimeSpan.FromMilliseconds(300);
var key = Keys.Right;

var keyUp = Observable.FromEventPattern<KeyEventArgs>(this, "KeyUp")
                      .Where(i => i.EventArgs.KeyCode == key)
                      .Select(_ => true);

var keyDown = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown")
                        .Where(i => i.EventArgs.KeyCode == key)
                        .GroupByUntil(k => 0, _ => keyUp)
                        .SelectMany(x => x.FirstAsync());

var keyDownDuration = keyDown.Select(k => keyUp.TimeInterval()).Switch();

var clicks = keyDownDuration.Where(i => i.Interval < limit);

var beginHold = keyDown.Select(k => Observable.Timer(limit).TakeUntil(keyUp))
                       .Switch();

var endHold = keyDownDuration.Where(i => i.Interval > limit);

/* usage */
clicks.Subscribe(_ => Console.WriteLine("Click"));
beginHold.Subscribe(_ => Console.WriteLine("Hold Begin"));
endHold.Subscribe(_ => Console.WriteLine("Hold End"));

WPF Version
Originally, I had mistakenly assumed the WPF flavour of KevEventArgs as IsRepeat is not available in the WinForms version - which means this won't work for OP, but I'll leave it in as it may be of use for others.
TimeSpan limit = TimeSpan.FromMilliseconds(300);
var key = Key.Right;

var keyUp = Observable.FromEventPattern<KeyEventArgs>(this, "KeyUp")
                        .Where(i => i.EventArgs.Key == key);

var keyDown = Observable.FromEventPattern<KeyEventArgs>(this, "KeyDown")
                        .Where(i => i.EventArgs.IsRepeat == false
                                && i.EventArgs.Key == key);

var keyDownDuration = keyDown.Select(k => keyUp.TimeInterval()).Switch();

var clicks = keyDownDuration.Where(i => i.Interval < limit);

var beginHold = keyDown.Select(k => Observable.Timer(limit).TakeUntil(keyUp))
                        .Switch();

var endHold = keyDownDuration.Where(i => i.Interval > limit);

/* usage */
clicks.Subscribe(_ => Console.WriteLine("Click"));
beginHold.Subscribe(_ => Console.WriteLine("Hold Begin"));
endHold.Subscribe(_ => Console.WriteLine("Hold End"));

To Test The Code
Include nuget package rx-main and paste the WinForms/WPF or code snippets as appropriate to the end of the Form contructor. Then run the code and press the right arrow key whilst observing the VS Output window to see the result.

redpajama_set_name: RedPajamaStackExchange


text:
Resources > Why Funding Circle? > News >
Funding Circle expands U.S. operations with new office in Denver
Updated: Apr 3, 2018
Funding Circle, the world's leading small business loans platform, today announced it is expanding its U.S. operations with a second office in Denver, Colorado. With the move Funding Circle will look to hire around 290 new employees in the Denver area over the next two years to support the company's growth.
Speaking about the move, Colorado Governor John Hickenlooper said: "Funding Circle's presence in Colorado helps strengthen our state's entrepreneurial and investor ecosystem. Their innovative platform provides greater access to capital and will help create sustainable job opportunities in tech and financial services — two of our state's fastest growing and most vibrant sectors."
J.J. Ament, CEO, Metro Denver Economic Development Corporation said: "We are proud that Funding Circle chose Denver for their new U.S. office. They represent a modern approach to business financing, making them a great fit for our region's financial and technology communities and they will have access to a diverse and talented workforce here."
Bernardo Martinez, U.S. Managing Director of Funding Circle, said: "Denver today serves as a hub for both the financial services and technology industries, making it an ideal second home for us in the United States. We are excited to become part of the Denver community and grow our team in the Mile High City."
At Funding Circle, businesses can obtain fixed-rate loans within days to purchase equipment and inventory, hire new employees or for other needs. With U.S. businesses now having accessed more than $1 billion in funding through the platform, Funding Circle is now the first lending platform anywhere in the world to have facilitated more than $1 billion across two markets.
Funding Circle US Launches Small Business Choice, Providing Interest Rate and Loan Term Options to Small Business Owners
Stress testing at Funding Circle
Decade after financial crisis, American small businesses move on from 'bank first' approach to financing

redpajama_set_name: RedPajamaCommonCrawl


text:
lap.2010.0147.pdf (120.81 kB)
Transumbilical laparoscopically assisted appendectomy: an alternative minimally invasive technique in pediatric patients
posted on 2011-05-27, 00:00 authored by Amy B. Stanfill, Danielle K. Matilsky, Kavitha Kalvakuri, Richard H. Pearl, Lizabeth J. Wallace, Ravindra K. Vegunta
Introduction: Transumbilical laparoscopically assisted appendectomy (TULAA) has been reported in the literature as an alternative to traditional three-port laparoscopic appendectomy (LA). Our study compares outcomes between LA and the one-trocar transumbilical technique in a single institution over a concurrent time frame for all cases of pediatric appendicitis. Methods: An Institutional Review Board–approved retrospective chart review of all appendectomies from July 2007 through June 2009 was performed. All appendectomies were performed either laparoscopically or transumbilically. One surgeon predominantly used the TULAA method, whereas the other 2 surgeons used strictly the LA method. No cases were converted to open. Categorization of specimens as normal, acute, or ruptured was based on pathology reports. Outcomes analyzed for each group included surgical duration, cost, length of stay, fever (>101.5F), wound infection, ileus, and postoperative abdominal-pelvic abscess. Results: A total of 131 appendectomies were performed by 3 surgeons, 83 were LA and 48 were TULAA. For all stages of appendicitis, outcomes differed significantly only for operating room cost, with the TULAA being significantly less expensive. All other outcomes were similar between the two techniques. Conclusion: Our study suggests that TULAA is a reasonable alternative to the standard minimally invasive technique for appendicitis in both acute and ruptured situations. All analyzed complications were similar between the groups, suggesting that TULAA is an acceptable surgical method in pediatric patients for all stages of appendicitis.
This is a copy of an article published in the Journal of Laparoendoscopic and Advanced Surgical Techniques © 2010 [copyright Mary Ann Liebert, Inc.]; Journal of Laparoendoscopic and Advanced Surgical Techniques is available online at: http://www.liebertonline.com. The original version is available through Mary Ann Liebert at DOI: 10.1089/lap.2010.0147
Mary Ann Liebert
Transumbilical laparoscopically assisted appendectomypediatric appendicitis


[Source: TGen] – A new light-speed computer connection using a military-tested network provides the Translational Genomics Research Institute (TGen) with the nation's fastest supercomputer link among life-science facilities.
This enhanced capability moves data up to 100 times faster between TGen and Saguaro 2, Arizona State University's supercomputer, accelerating TGen's molecular research into diseases such as Alzheimer's, diabetes and many types of cancer.
To make this possible, Cox Business Arizona has installed a 10-gigabit LightWave fiber-optic connection between TGen in downtown Phoenix and the Saguaro 2 supercomputer, 10 miles away at Arizona State University's main campus in Tempe.


text:
Colin Quinn coming to the Egg with simple plan: Get laughs
Steve Barnes
Comedian Colin Quinn will bring his new show, "The Last Best Hope," to The Egg in Albany on Jan. 14, 2022. The show ran off-Broadway for three weeks in November 2021.
Provided photoShow MoreShow Less
Mike Lavoie photoShow MoreShow Less
"If you advertise as a comedian," said Colin Quin, "your first job is to elicit laughs. With doctors, it's first do no harm. With comedians, first get laughs."
Over the past two decades, Quinn's shows have included six with multiweek runs, or longer, in off-Broaday theaters, and they were often described as comedic monologues with social observations both shrewd and philosophical. He brings the latest, "The Last Best Hope," to The Egg in Albany on Jan. 14, about two months after closing its stay at the Lucille Lortel Theatre in Manhattan's West Village.
But, he said during a phone chat from his New York City home last week, "There's still a lot of stand-up in it, because I'm a stand-up comedian." A "Saturday Night Live" cast member from 1996 to 2000, from 2002 to 2004 he hosted "Tough Crowd with Colin Quinn," a celebrated talk show on Comedy Central featuring Quinn discussing current events with a rotating panel of comedians.
Among topical subjects in "The Last Best Hope": COVID-19, which Quinn had. Twice. The first time, prior to vaccine availability, he was medically eligible to receive monoclonal antibodies. He said, "It was pretty bad at the start, but the treatment changed everything for me." The second COVID infection, after he'd received a booster shot, was milder, he said, "But you still feel terrible."
He's resigned about the progress of the virus: "There's no hiding from it. I feel like everybody is going to get it. We're doing herd immunity on the installment plan."
In part of "The Last Best Hope," Quinn revisits the divided America he examined in his 2019 show, "Red State Blue State," which started on stage and went on to stream on Netflix and was also the first comedy special ever aired by CNN. At its most basic level, the split is between those who support former President Donald Trump and those who don't.
"I feel like that gulf has gotten even bigger," Quinn said. "People in this country are so entrenched, and it's to the point where a lot of people are unwilling to even listen to the other side."
That change in outlook, Quinn said, is both a cause and a result of the amplifying effect of social media.
"Before, if you wanted to talk to somebody, a friend or a stranger, about what was going on in the world, you had to go to a bar and start a conversation," Quinn said, "Now, you don't even have to leave your house. You just post it on social media. It's like everybody is just standing at home and yelling out the window at the same time, all the time."
Quinn said he is bothered by the seemingly instantaneous outrage and opprobrium evoked by even mild statements from celebrities, comedians included.
"It's insane how much less tolerant people are," he said. "It's like almost everything you say is going to be seen by somebody as controversial." He acknowledged that he on occasion reconsiders saying something from the stage today that he freely would have said while riffing with the likes of Jim Norton, Patrice O'Neal, Judy Gold and Nick Di Paolo on "Tough Crowd with Colin Quinn."
"Sometimes you'll just get shocked silence, and I still do some of that," he said. "But sometimes I now think, 'Do I want to ruin everybody's night?' If you're a comedian who's doing you're job, you find a way to put it into joke form to make people laugh."
Colin Quinn: "The Last Best Hope"
When: 8 p.m. Friday, Jan 14
Where: Swyer Theatre, The Egg, Empire State Plaza, Albany
Info: 518-473-1845 and theegg.org/events
Reach Steve on
Steve Barnes has worked at the Times Union since 1996, served as arts editor for six years, and since 2005 has been a senior writer. He generally covers restaurants, food and the arts, and is the Times Union's restaurant columnist and theater critic. Steve was also a journalism instructor at the University at Albany for 12 years. You can reach him at sbarnes@timesunion.com or 518-454-5489.


text:
package com.kanomiya.steward.model.overlay.text;

import jdk.nashorn.api.scripting.ScriptObjectMirror;

/**
 * @author Kanomiya
 *
 */
public interface IConfirmable<T> {

	boolean hasConfirmHandler();
	ConfirmHandler<T, ConfirmResult> getConfirmHandler();

	// ConfirmResult confirm();

	default ConfirmResult confirm(T arg)
	{
		if (! hasConfirmHandler()) return null;
		if (! isEnabled()) return null;

		return getConfirmHandler().apply(arg);
	}

	IConfirmable confirmHandler(ConfirmHandler<T, ConfirmResult> confirmHandler);

	default IConfirmable confirmHandlerJS(ScriptObjectMirror jsFunction)
	{
		return confirmHandler(ConfirmHandler.create(jsFunction));
	}

	boolean isEnabled();
	IConfirmable enabled(boolean enabled);

}

redpajama_set_name: RedPajamaGithub


A thoughtfully designed three bedroom detached 'new build' bungalow pleasantly situated within walking distance of the centre of this most sought after Lincolnshire village. The property will be constructed by I. J. Building Contractors, known for their high standard of fitment and attention to detail. The accommodation comprises stylish open plan dual aspect kitchen, bay fronted living room, utility room and en-suite to master bedroom. At this stage the purchasers would enjoy choice of fitment design.


text:
Infantry regiments; crucible of camaraderie
by Sudhir Sharma July 17, 2014
written by Sudhir Sharma July 17, 2014
Irecently attended a unit reunion where young officers with barely six days service mingled intimately with ramrod stiff colonels with more than six decades of regimental affiliation. Amidst much bonhomie and exchange of nostalgic anecdotes the warmth of the extended yet cohesive family spirit was heartwarming. Middle aged ladies who had joined the unit as young brides affectionately hugged and paid respects to the older ladies, who had once been their CO's wives and had welcomed them and initiated them into the fold of the close-knit unit life. I feel that for the infantry which relies so heavily on espirit -de – corps and motivation to overcome the travails it faces, the regimental system provides it the perfect foil to mould and temper the camaraderie and trust so vital in waging and winning wars.
Some researchers have gone to the extent to say that the regimental system was perhaps one of the main factors in the British victory in the Falklands. It may be recalled that the British had to travel 8000 miles across the oceans to do an opposed amphibious assault without air superiority, yet defeated the Argentinean army which is constituted on the continental (industrial) concept.
The French word regiment came into proper usage in the 16th century wherein retinues followed Knights into battle and units were named after their commanding colonels and were normally disbanded after a campaign. Based on this tradition regiments often recruited from their regions known as cantons. The oldest known regiments some of which still exist till date are; Swedish life Guards 1521 and the British Honourable Artillery Company 1537, the oldest being a regiment established in Spain in 1248. In the olden days regiments generally had a maximum of 2 to 4 battalions and recruited from the same region for added cohesion. The colonel of the regiment was the elder statesman who inspired and guided the regiment as also oversaw their training, dress, accruements and traditions. This tradition thankfully survives till today.
The Indian infantry regimental system is of course the legacy of the British who created the same, based on the now outdated notion of martial races. Fortunately the system in India evolved over time and we moved from fixed class to mixed class and finally graduated to the all India class system. The concept of Nam, Namak and Nishan truly epitomizes the very soul of the Infantry regimental system. The izzat of the paltan being foremost is drilled into the mind of each soldier and the respect and reverence granted to regimental colours is possibly second only to the tricolor. The first initiation of a person into the way of life of the army takes place at the regimental centre. Here the tough initial training and traditions of the regiment are handed down to the next generation of soldiers creating a strong and unshakeable life long bond. Twenty or more years later the same battle hardened soldier returns to his alma mater; the regimental centre, to hang up his uniform and go home. But the relationship endures much beyond, not only lasting a lifetime but often spanning many generations.
It is therefore vital that this time and war tested structure is preserved, strengthened and made relevant in the modern context. Periodically, people with limited understanding of the underlying ethos of the regimental system come up with suggestions of new regiments on regional cum caste and ethnic basis. This is unacceptable and has been rightly rejected outright by the army hierarchy. The Infantry regimental system is the bedrock of our organizational structure which has stood the acid test of many challenges in wars and peace over many decades. It symbolizes the heart, soul and perhaps the very spirit of the Indian Infantry. It is therefore our duty to nurture and cherish this unique institution for the continued well being of our gallant army!
Lt Gen Sudhir Sharma,PVSM, AVSM, YSM, VSM,(Retd) is the Chairman of MitKat Advisory Services, India's leading premium risk consultancy. He hails from the Brigade of Guards.
Sudhir Sharma
Lessons from a war we've all but forgotten
DBO: WHOSE PERFIDY IS THE BORDER IMBROGLIO?


Looking for the BEST half season program in Clay County? Our info packet will be out November 5th 2018! NEW THIS SEASON- SIGN-UP IN NOVEMBER AND RECEIVE 5 MONTHS OF FREE TUMBLING! 1 practice a week. 2 local competitions. Half the cost. Same top-notch coaching staff!


PlayStation and Sony San Diego just released the first trailer for MLB The Show 19, this year's installment of the popular baseball sim. The gameplay trailer includes glimpses of new gameplay, as well as some returning legends.
You can see glimpses of new and returning features coming to MLB The Show 19. Room customization and Legends will be making a comeback, while the core gameplay looks to have gotten some exciting updates. Sony San Diego has always prided itself on the lifelike gameplay of MLB The Show, and there seems to be some added refinements since last year's installment. The trailer also ends on a cool shot of baseball legend Willie Mays in a black-and-white filter.
MLB The Show 19 features Bryce Harper as its cover star, despite him being a free agent in 2019. The final cover will likely change to reflect which MLB team he ultimately signs with for the season, but until then the cover simply features Harper in a hoodie.
MLB The Show 19 launches on March 26, 2019 and comes in two editions. The Standard Edition is available for pre-order and comes with 10 Standard Packs along with some retail-specific bonuses. You'll be getting the most by pre-ordering from the PS Store which includes a Gold Player Choice Pack, 5,000 Stubs, and the Bryce Harper MVP Flashback Card for MLB The Show 18.
Check out our MLB The Show 18 review for our take on Sony San Diego's last baseball outing, or our MLB The Show 18 guide for tips and tricks before the new season begins.
Tagged with News,	PlayStation 4,	Sony,	Sony San Diego,	Sports.
Not just the best baseball sim of the generation, but one of the best sports sims period.


text:
The 2009 Los Angeles Streetsies – The Worst of Times
(I didn't say it yesterday, so let's say it today. Thanks to Dorothy, Mark, Marybeth and Matthew for being on the panel.)
Worst News of the Year
Panel's Pick: Despite State Budget Crisis, State Won't Raise Gas Tax But Will Cut Transit
My Pick: Despite State Budget Crisis, State Won't Raise Gas Tax But Will Cut Transit
This story qualifies for a number of reasons. First, of course, there's a budget crisis and that stinks, especially for state workers who are losing their jobs or getting furloughed. But of course the real reason for its inclusion here is because the massive cuts to transit agencies around the state, including OCTA, and Metrolink as well as future cuts coming to DASH and Metro.
Of course, there's also the sad state of affairs for our legislature. They talk tough on the environment but still cut transit while expanding highways across the state.
Honorable Mention: City Does Nothing to Stop Speed Limit Increases, Metro Installs Turnstiles for No Apparent Reason
Worst Law Enforcement Story That Doesn't Involve Andres Tena
Panel's Pick –
LAPD Tackle Cyclist at Critical Mass
My Pick –
LAPD Teaches USC Security How to Enforce Non-Existant Crosswalk Ban on Cyclists
There's nothing worse than the police poorly enforcing laws…unless it's the LAPD enforcing non-existent laws and teaching private security to do the same.
Honorable Mention – LAPD in Mid-Wilshire – Ticket Bikes and Peds in the Crosswalk, Ignore Red Light Runners, LAPD Doesn't Know Crosswalk Laws, Sherrif's Start Checking Bags at Train Stations, Metro Station's No-Man's Land at Night
Worst Commercial
Panel's Pick: Jack in the Box Parodies a Pedestrian Getting Creamed by a Bus
My Pick: Audi Makes Fun of Alternative Transportation
As much as I'd like to believe the panel was just having my back against the noxious Jack in the Box Spammers, I have to confess that I forgot about this Audi ad or I'm sure they would pick that. It's perhaps the most obnoxious car ad I've ever seen.
Honorable Mention: Mercedes: Wreckless Driving is Awesome, Toyota's Ransom Note to America
Most Disheartening Statement by LADOT at a City Council Hearing
Panel's Pick: City's Racial Diversity a Reason for Lesser Outreach on Bike Master Plan
LADOT Is "Out of the Loop" on BMP Release Date/Strategy
By now, news of LADOT Senior Bike Coordinator Michelle Mowery's gaffe has reached Portland, New York and beyond. Yes, she really did say that the reason our bike planning is so far beyond Portland is because we're not white enough. I still can't believe that's what she actually meant, but she said it so there it is.
I'm going with the LADOT complaining on being "out of the loop" on the Bike Plan release last summer because it's something they clearly meant. If it's just an example of the LADOT blowing gas, it's disheartening they're trying to wash their hands of the plan before it was even fully released. If true, it shows a shocking lack of coordination on a document that could end up being more important to cyclists in Los Angeles over the next decade than any other.
Honorable Mention: Concerns Over Slipperiness of Paint a Reason for Not Painting Sharrows, City doesn't have the bike facilities to make bike share worthwhile
Worst Press Coverage
Panel's Pick: Times' Rutten Hands Out Misinformation on Congestion Pricing
My Pick: Media Refuses to Ask Questions on Road Widening of 405
Wow, re-reading that Rutten piece it's hard to believe he got paid to write that. And they can't afford Steve Hymon? Jeesh.
That being said, the media's inability to be more than stenographers when it comes to writing a story about a highway expansion project and how awesome it's going to be is a more disturbing trend. It seems that if a state official, elected or otherwise, says that a widening is going to be good for the environment and congestion, well, that's good enough for the press.
Honorable Mention: LA Now Complains about Traffic "Snarls" in Calmed Santa Monica, WaPo Features HSR Hack Job, Everyone Has a Laugh at Shemar Moore's Bike Crash, ABC 7 Asks Drivers if They're Scared of Cyclists, Industry Rag Is Shocked That CAA Wants Nobodies to Pay for Parking
Story That Never Seems to End
Panel's Pick: Metro Spends Entire Year Debating AnsaldoBreda and Long Range Transportation Plan,
Orange County Cutting Transit, Expanding Highways, The Pico-Olympic Plan Stumbles Forward
Metro Spends Entire Year Debating AnsaldoBreda and Long Range Transportation Plan
What we really can learn from this category is that bad ideas never die in government, they're just waiting to pop back up and bite us again. That being said, for me the story that sucked up more oxygen was the Metro Board's inability to make the hard choices on AnsaldoBreda or passing the Long Range Transportation Plan after Measure R pretty much made all the long term planning decisions for them.
The AnsaldoBreda saga was just too pathetic for words. Month after month the Board was faced down by an army of hard hats despite overwhelming evidence that AnsaldoBreda was the wrong company to make the Measure R rail cars. The promise of building a factory locally was too much for the Board to ignore, even though legally they couldn't consider it if they want to keep receiving federal funds. Mercifully, after nine months of political spinlessness, AnsaldoBreda decided they could get a better deal by going through an open bidding process than bullying a bunch of politicians. Hopefully, I'm not going to have to type the words AnsaldoBreda too many more times, but we'll see what the bids to construct Metro's rail cars look like when they come in.
Honorable Mention: City to Spend 10% of It's Measure R Local Return on Bike/Ped., Villaraigosa Thinks About Privatizing Parking,
Everybody Hates the Bike Plan
Bad News from Sacramento
Panel's Pick:
Governor/Legislator Screw Everyone and the Environment in the Name of Building Football Stadium
My Pick: Governor/Legislator Screw Everyone and the Environment in the Name of Building Football Stadium
Yeah, they actually exempted a 100,000 seat NFL football stadium from having to undergo a full environmental review. Honestly, how anyone involved in this debacle can ever claim to give a damn about the environment and air quality without getting laughed at ever again is beyond me. Because the only review for the stadium was approved only by a political body who's members will benefit financially from the stadium's construction; we'll never see an honest assessment of what damage will be done by the traffic and construction of this football megalopolis. It's too bad Ed Roski doesn't care as much about people dieing on the streets because of speeding drivers as much as he does conning the legislature into giving him a sweetheart environmental deal.
The only good news out of this is that my Assemblyman didn't vote for the exemption. I'm going to pretend it's because I called their office everyday and complained about it. It makes me feel better.
Honorable Mention: Safe Streets Legislation Bottled in Committee, CALPIRG/Smart Growth America Slam State's Stimulus Priorities, Governor Rejects Legislation Requiring 710 Expansion to Run Underground
Biggest "Duh" of the Year
Bike Counts Hint at Chronic Under-counting of City's Cyclists
My Pick: City "Opens Crosswalk to Pedestrians"
I get that it's not a surprise that the city has no idea how many people are biking; but c'mon…it was a crosswalk with a no pedestrians sign!
Honorable Mention: Widening Highways Lead to Faster Speeds
Dumbest Quote
Panel's Pick: Attorney for Man Who Ran Over Pedestrian with Hummer Before Beating Him, "random interactions of diverse people in a
city as frenetic as Los Angeles can lead to disastrous consequences."
Villaraigosa Cites Congestion as a Reason to Subsidize Cars
The best thing about these two quotes? They weren't off the cuff or parts of extemporaneous comments, they were out of the press release in the Mayor's case and court filings in the attorney's case.
Honorable Mention: Frank McCourt Derides City's $300,000 investment in transit for Dodgers as "not real transit" and "a few buses", Boxer Floats "Honor System" As Best Way to Have VMT Tax, Opponent of Highway Memorials Doesn't Want Highways "Cluttered" with Memorials, Rita Robinson Rips City Council to Distract Communities from LADOT's Lame Outreach
Best Example of Screwing the Community
Panel's Pick: Traffic Calming Ripped Out in Westwood
My Pick: Traffic Calming Ripped Out in Westwood
I know there's two sides to every story, but in this case the city actually took out traffic calming because people on adjacent streets were complaining the traffic calming was working. Sounds like an open and shut case that the rest of the community needs calmed streets to divert traffic on to the arterials. The city didn't see it that way.
Honorable Mention: Locals Hate Locally Preferred Alternative for Route 2 Terminus, Fans of the Transit Hating Dodgers Pack Local Streets to Avoid Paying for Parking, Burbank Removes Street Parking to Increase Car Capacity
Is $100 Million Enough to Hold on July's Metro Fare Hikes?
By Damien Newton	| Apr 13, 2010
Are we racing to fare hikes for no reason? Photo: As part of the compromise allowing Governor Schwarzenegger to eliminate the gas tax and replace with with an excise tax (ABX8 6 and ABX8 9), monies collected from the diesel tax are being used to partially replace the funds being removed from the State Transit […]
From Russia, with Transit Love
By Alexander Friedman	| Jun 29, 2009
View of a departing Moscow subway train. All Photos: Alexander Friedman I just returned from a trip to Moscow and noticed an interesting trend. Despite the economic slowdown, which Russia is also certainly experiencing, their public transportation is not only as efficient as it's always been, but – it keeps getting better and better. Unlike […]
The U.S. Transportation Financing Crisis: A Snapshot From the States
By Elana Schor	| Jan 7, 2010
Washington transportation policymaking can often resemble an unwieldy soup of anywhere between 50 and 535 local perspectives, as lawmakers from different states and districts vie for a fixed (or even shrinking) amount of federal funding. Congress isn't eager to raise fuel taxes to pay for transportation — but what about the states? (Photo: Pop and […]
By Damien Newton	| Dec 31, 2009
Bike Story of the Year: Panel's Pick: Everybody Hates the Bike Plan, The Trial and Conviction of the Road Rage DoctorMy Pick: Hummer Slams Into Cyclist, LAPD Blames Cyclist, Cyclists Swarm City Council and Police Commission I'm not even sure I need to go in to an explanation for any of these stories. The panel […]
State Budget: Higher Fees Won't Lead to Better Transportation
By Damien Newton	| Feb 12, 2009
For those of us awaiting our tax return from the State of California, the news from Sacramento concerning yesterday's budget compromise comes as welcome news. For just about everyone else, the news isn't good. That's especially true for anyone that has a commute that isn't either a pedestrian or cyclist. You're not going to catch […]
Cal Transit Association on State Budget: Armageddon Is Here
Yesterday, transit advocates from around the state strove to grapple with the new reality that Governor Arnold Schwarzenegger and the state legislature's "compromise" on the state budget completely abolished the State Transit Assistance (STA) and the $536 million that it dedicated to subsidizing transit operations. There are multiple villains in this story, but the blackest […]


1. Successful team creates a successful tour. African Angel Tours offers the personal hands-on approach. We deal with each visitor as part of the family.
2. Luke Angel holds a degree in political science and international relations (Wits). He is currently working in partnership and strategic alliance with Grassroots Tourism. Luke began marketing South Africa in the early 1990's, representing South African arts and crafts products in Trade shows in Germany, France and Italy. Over the past eight years he has focused on the United States market and has built up an extensive business network and client base in California, especially within the African-American community.
3. Luke and his team have dedicated their lives to dismantling racial myths, borders and fears: believing and actively gearing all their business operations to the philosophy that cultural exchanges are the best way to build relations across the social, economic and political divides.
1. The ultimate aim is to not facilitate more low skilled jobs in an industry dominated by a few established big-business players. AAT believes the whole point is to empower talented previously disadvantaged tourist business individuals to reach out and offer their product and services to the tourist and thus directly benefit from the growing volume of tourist trade to South Africa. By virtue of the incredible Rainbow people, world renowned for their hospitality and humanity, AAT believes tourism to South Africa should focus on cultural exchanges and offer the opportunity to the visitor to rub shoulders with the locals: thus contributing to this new "Tourism Way". The visitor and the new tourist product of South Africa have the potential to build "the Better life for all" (Nelson Mandela Feb., 1994) in South Africa: AND portray South Africa as it really is. AAT USA Inc. will thus be the vehicle to exploit this dynamic niche in the expanding tourism market.
1. Conclusion. Collectively Luke and his team are focused on expanding the tourism industry to South – and Southern Africa AND growing an understanding - and experience of the visitor to South Africa to become more than a "bus window" experience. Building on their extensive international business and client connections, South and Southern African networks into the community development, craft production and tourism industries, incredible and diverse tourist attractions: their intimate knowledge of the high expectations of international consumers of cultural products, AAT is developing an exciting new generation of South African cultural tourism packages which will include the mainstream "Must see" attractions: but will include true Journeys of the Mind: into "Must do" experiences. After years of directly marketing South African products overseas, the team has the knowledge and experience of how to use cultural tourism to build bridges between our country and others, opening up those elusive trade linkages that really mean economic empowerment for poor communities. It is the objective of all partners to redefine tourism: both for the visitor to South Africa, exposing them to the country as it is, as well as their interaction and with South Africans themselves as the hosts.


text:
Q: How to request to Gunicorn and respond with Daphne? Using Django channels, Nginx, Gunicorn, Daphne I'm facing a strange issue in deploying Django app with Nginx, Daphne and Gunicorn.
As of now, I have successfully deployed my Django app.
My HTTP requests are handled by Gunicorn, and WSS requests are handled by Daphne, which is meant to happen.
Consider a scenario, where I send an HTTP request to Gunicorn, and Django needs to send a message back via channels. How do I do that?
For example, if I add a post via HTTP POST request, and want to send a notification via channels to all the open channels (which I have a list of) how do I make Django send back a channel message through Daphne, even when the request was HTTP in the first place received through Gunicorn?
The request flow that I need is:
Client  >  HTTP  >  NGINX  >  GUNICORN  >  DJANGO  >  Do some processing  >  Send notification to all open channels via WSS >  Send HTTP RESPONSE OK STATUS=200 to client
I used this guide to deploy
https://github.com/mitchtabian/HOWTO-django-channels-daphne/blob/master/README.md
Everything is working fine except I don't receive WSS messages on the client WebSocket listener, when I send a HTTP request to Gunicorn, but when I send HTTP request to Daphne I get a WSS response successfully

A: You need to configure a Channel Layer:

Channel layers allow you to talk between different instances of an application.

channels_redis is a good one, to transfer a message from a server to another.
The request flow will be:
Client request -> NGINX -> Gunicorn -> Django Application -> Send notification  -> Redis -> Websocket Consumer -> Send response


text:
In 100 Words: Indefinite Hiatus
Billboard Hot 100: December 8, 2018
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Tag Archives: Bubble Gang
comedy, humor, Philippines, television
Ogie Added to Home Sweetie Home, Now Up Against Bitoy Every Saturday
March 15, 2018 ralphierceABS-CBN, ASAP, ASAP ABS-CBN, Bubble Gang, Bubble Gang GMA, Cheche Bureche, Cheche Bureche Bubble Gang, Erich Gonzales, GMA, GMA Network, Home Sweetie Home, Home Sweetie Home ABS-CBN, Jodi Sta. Maria, Michael V, Ogie Alcasid, Pepito Manaloto, Pepito Manaloto GMA, Pepito Manaloto: Ang Tunay na Kwento, Pepito Manaloto: Ang Tunay na Kwento GMA, Pickup Lines, Pickup Lines Bubble Gang, Piolo Pascual, Rufa Mae Quinto, Sana Dalawa ang Puso, Sana Dalawa ang Puso ABS-CBN, Sexballs, Sexballs Bubble Gang, The Blood Sisters, The Blood Sisters ABS-CBN, Toni Gonzaga, TV5, Yaya and Angelina, Yaya and Angelina Bubble Gang 4 Comments
From co-stars to opponents.
Ogie Alcasid and Michael V were the faces of 'Bubble Gang' for a majority of the show's run. Many of 'Bubble Gang"s most memorable skits were focused on the two men, whether it is Yaya and Angelina, Cheche Bureche, Pickup Lines or the Sexballs boy group.
However, Ogie moved on from 'Bubble Gang' and GMA in 2013, and after a three-year spell with the then-TV5, he joined ABS-CBN last year. Yet despite gaining prominence as an occasional performer on 'ASAP', as well as composer and judge on several ABS-CBN talent shows, the itch to rekindle those comedic chops soon got the best of him.
Earlier this week, ABS-CBN announced that Ogie Alcasid would be added to the cast of the sitcom 'Home Sweetie Home'. Ogie's role in the sitcom will be similar to what Jodi Sta. Maria ('Sana Dalawa ang Puso') and Erich Gonzales ('The Blood Sisters') do: that of playing at least two different characters.
Ogie Alcasid also joins the cast of "Home Sweetie Home." He will play the roles of Ireneo and Irenea.
A post shared by ABS-CBN PR (@abscbnpr) on Mar 13, 2018 at 2:24am PDT
In 'Home Sweetie Home', Ogie plays fraternal twins Ireneo (or Neo) and Irenea. Neo is the childhood friend of Julie (Toni Gonzaga) who soon invests on the Walang Kapares pastry shop and restaurant, while Irenea becomes JP's (Piolo Pascual) love interest.
With the addition of Ogie Alcasid to 'Home Sweetie Home', a very interesting subplot develops. 'Home Sweetie Home"s archrival 'Pepito Manaloto' stars Michael V, and his former 'Bubble Gang' co-stars in Ogie and Rufa Mae Quinto now find themselves on the opposite side of the fence.
But don't expect this rivalry to be a fierce one. In fact, off the camera, Ogie, Bitoy and Rufa Mae remain friendly to each other despite their different network allegiances.
Already one of ABS-CBN's top-rated programs on Saturday nights, 'Home Sweetie Home' could only get better with the entry of one of the Philippines' most versatile talents. Still, it will be interesting to see how Ogie's addition will spice up this popular sitcom.
anime, entertainment, Philippines, television
Newly-Redubbed Voltes V to Air on GMA Astig Authority This Week As Part of 40th Anniversary
March 26, 2017 ralphierce1986 EDSA Revolution, 1986 People Power Revolution, ABS-CBN, Ang Dating Daan, Ang Dating Doon, Astig Authority, Bea Binene, Big Bert Armstrong, Boazanians, Bubble Gang, Bubble Gang GMA, Commander Robinson, Daimos, Derrick Monasterio, Dr. Hook, Dr. Ned Armstrong, Dr. Smith, Draco, Ferdinand Marcos, GMA, GMA Astig Authority, GMA Network, Hero, Hero channel, Hero TV, Hero TV channel, Hiro Peralta, IBC-13, Intercontinental Broadcasting Corporation, Jak Roberto, Jamie Robinson, Jett Pangan, Ken Chan, Little John Armstrong, Magandang Buhay, Magandang Buhay ABS-CBN, Mark Gordon, Mary Armstrong, Nippon Sunrise, People Power Revolution, President Ferdinand Marcos, Prince Zardos, Sandara Park, Steve Armstrong, Tadao Nagahama, Telesuccess Productions, Toei Animation, Voltes V, Voltes V 40th anniversary, Voltes V ABS-CBN, Voltes V banned, Voltes V banned by Marcos, Voltes V Evolution, Voltes V GMA, Voltes V IBC-13, Voltes V new dub, Voltes V redub, Voltes V rerun, Voltes V: The Liberation, Zandra, Zuhl 3 Comments
The legend returns: 'Voltes V' will air anew on GMA starting tomorrow, featuring a new Filipino dub. (Screenshot courtesy of Toei, Nippon Sunrise, Telesuccess Productions and GMA Network)
'Voltes V' is back.
As part of the anime's 40th anniversary, GMA announced that a redubbed version of 'Voltes V' will be aired starting tomorrow morning on the network's Astig Authority block. The fresh new Filipino dub of 'Voltes V' will feature the following Kapuso stars:
Derrick Monasterio (as Steve Armstrong)
Jak Roberto (as Mark Gordon)
Hiro Peralta (as Big Bert Armstrong)
Bea Binene (as Jamie Robinson)
Ken Chan (as Prince Zardos)
The voices of Little John Armstrong, Dr. Ned Armstrong, Mary Armstrong, Dr. Smith, Commander Robinson, Dr. Hook, Draco, Zandra, Zuhl and other characters will also be dubbed by various voice actors. However, it is unknown if GMA will give credit to these voice actors for their roles in the redubbed 'Voltes V'.
'Voltes V', a brainchild of Tadao Nagahama (also the man behind 'Daimos'), was first aired on GMA in 1978. However, it was soon banned by then-President Ferdinand Marcos (along with the aforementioned 'Daimos' and other robot anime) for being 'too violent'.
It was only after the People Power Revolution that 'Voltes V' triumphantly returned on air through various TV channels (most notably ABS-CBN and IBC-13). In 1999, 'Voltes V' was brought back by GMA in both English and Filipino dubs (along with the four unaired episodes known as 'Voltes V: The Liberation'), and instantly became popular with a new generation of viewers.
The renewed popularity of 'Voltes V' also inspired the 'Bubble Gang' segment 'Ang Dating Doon' (a parody of the religious program 'Ang Dating Daan') to adopt the anime's opening song as its theme. The skit even brought a toy version of the robot on numerous occasions.
In 2005, ABS-CBN's Hero channel aired a new Filipino dub of 'Voltes V' (known as 'Voltes V Evolution'), starring the likes of Jett Pangan and Sandara Park. However, the new dub was poorly received.
The 2017 dub of 'Voltes V' may be a celebration for the anime's 40th anniversary, but it remains to be seen if the redub will be worth watching. Considering that 'Magandang Buhay' has had some winning moments in recent episodes, this will be a tough task for the returning series to overcome.
Still, for those too young to remember the battle between the Boazanians and the Earth's special forces, 'Voltes V' will be a new experience for all of them. Win or lose, this new dub should give them a fresh perspective on how good overcomes evil, and how bloodlines were revealed.
entertainment, Philippines, reality show, talent show, television
In 100 Words: Ogie Alcasid Officially a Kapamilya
February 1, 2017 ralphierceABC 5, ABS-CBN, Associated Broadcasting Company, Bubble Gang, Bubble Gang GMA, GMA, GMA Network, It's Showtime, It's Showtime ABS-CBN, Ogie Alcasid, Ogie Alcasid ABS-CBN, Ogie Alcasid joins ABS-CBN, Ogie Alcasid Kapamilya, Ogie Alcasid new contract, Ogie Alcasid new contract with ABS-CBN, Regine Velasquez-Alcasid, Tawag ng Tangahalan It's Showtime, Tawag ng Tanghalan, Tropang Trumpo, Tropang Trumpo ABC-5, Your Face Sounds Familiar: Kids, Your Face Sounds Familiar: Kids ABS-CBN 1 Comment
Say hello to new ABS-CBN star Ogie Alcasid.
The multi-awarded singer-songwriter officially inked a contract with ABS-CBN yesterday. formally welcoming him on the Kapamilya camp. This is now his second stint with ABS-CBN after starting his entertainment career with the said network over two decades ago.
Ty so much for the warm welcome @abscbnonline #abscbn #excited #overwhelmed #grateful #humbled #tyLord
A post shared by Ogie Alcasid (@ogiealcasid) on Jan 29, 2017 at 10:41pm PST
Ogie, who also carved a career as a comedian on shows like ABC-5's 'Tropang Trumpo' and GMA's 'Bubble Gang', is currently a judge on 'Your Face Sounds Familiar Kids' and 'It's Showtime"s Tawag ng Tanghalan. He also joins a rare list of couples who work on separate networks, as wife Regine Velasquez-Alcasid remains under contract with GMA.
comedy, entertainment, humor, Philippines, television
The End of A1 Ko Sa 'Yo?
November 29, 2016 ralphierce#A1ThankYou, A1 Ko Sa 'Yo, A1 Ko Sa 'Yo break, A1 Ko Sa 'Yo cancellation, A1 Ko Sa 'Yo conclusion, A1 Ko Sa 'Yo final episode, A1 Ko Sa 'Yo finale, A1 Ko Sa 'Yo GMA, A1 Ko Sa 'Yo last episode, A1 Ko Sa 'Yo offseason, A1 Ko Sa 'Yo season break, A1 Ko Sa 'Yo season finale, ABS-CBN, Bubble Gang, Bubble Gang GMA, Denise Barbacena, Facebook, Gardo Versoza, GMA, GMA Asianovelas, GMA Heart of Asia, GMA KiliTV, GMA Koreanovelas, GMA Network, Jaclyn Jose, Jane the Virgin, Jane the Virgin ABS-CBN, KiliTV, Pinoy Big Brother, Pinoy Big Brother ABS-CBN, Pinoy Big Brother: Lucky 7, Pinoy Big Brother: Lucky 7 ABS-CBN 17 Comments
Is it really over for 'A1 Ko Sa 'Yo'?
Last Thursday, the GMA-produced sitcom aired its so-called season finale, with the hashtag #A1ThankYou. In the episode, Digna (Jaclyn Jose) held a Thanksgiving party to celebrate the return of Kaycee (Denise Barbacena) and her reconciliation with her husband Rolly (Gardo Versoza).
While the episode probably signaled the conclusion of 'A1 Ko Sa 'Yo' as viewers know it, the show's Facebook page tried to pacify concerns by promising another season in the near future. Still, any hope of a return may be questionable considering the year-round approach of Filipino programming.
'A1 Ko Sa 'Yo' first premiered on June 2, 2016 as GMA's first weeknight sitcom in nearly a decade. The show immediately lived up to its billing as a sexually-themed sitcom, emphasizing more mature situations that are not suitable for young kids.
The premiere of 'A1 Ko Sa 'Yo' was at the time an attempt to revive the seemingly moribund KiliTV block. In fact, GMA promoted both 'A1 Ko Sa 'Yo' and 'Bubble Gang' in various 30-second clips in order to draw interest from comedy fans.
Unfortunately for GMA, 'A1 Ko Sa 'Yo' did not exactly revive the glory days of KiliTV. The sitcom initially fared well against ABS-CBN's Filipino-dubbed 'Jane the Virgin', but it soon lost momentum against the ever-popular yet controversial 'Pinoy Big Brother: Lucky 7'.
Let's face it, 'A1 Ko Sa 'Yo' was not a bad sitcom. It was a fresh concept that is as daring as it can get, in contrast to more family-oriented sitcoms that viewers became accustomed to.
'A1 Ko Sa 'Yo' also helped GMA reduce its overdependence on Koreanovelas. During its 26-episode run, various GMA Koreanovelas run for only three times a week, enabling it to run a lot longer than usual.
But now that the sitcom is all but history (for now), expect Koreanovelas to once again dominate GMA's late-night programming. It's a sad development considering that GMA wanted to place another show on weeknights to alleviate its burden on Koreanovelas.
It remains to be seen if 'A1 Ko Sa 'Yo' will ever come back as promised. But for now, it's good riddance for the show.
In 100 Words: Bubble Gang's 21-Gang Salute Special
November 25, 2016 ralphierceAntonio Aquitania, Arianne Bautista, Arra San Agustin, Bubble Gang, Bubble Gang 21st anniversary, Bubble Gang 21st anniversary special, Bubble Gang 21st season, Bubble Gang anniversary, Bubble Gang anniversary episode, Bubble Gang anniversary presentation, Bubble Gang anniversary specials, Bubble Gang GMA, Bubble Gang new opening billboard, Bubble Gang opening billboard, Bubble Gang special episode, Bubble Gang specials, Bubble Gang: 21-Gang Salute, GMA, GMA Network, Gwen Zamora, Jak Roberto, Joyce Ching, Kim Domingo, Max Collins, Michael V, Rufa Mae Quinto, Sam Pinto, Valeen Montenegro 2 Comments
All hail 'Bubble Gang'!
On November 25 and December 2, the Philippines' longest-running gag show will celebrate its 21st year on air with a two-part special. Titled 'Bubble Gang: 21-Gang Salute', the episodes will feature skits that will pay tribute to the country's greatest comedians of all time.
'Bubble Gang' has been on the air since October 20, 1995, with Michael V and Antonio Aquitania the only remaining holdovers from the show's first episode. Last year saw the departures of Sam Pinto, Max Collins, Rufa Mae Quinto, Gwen Zamora and Joyce Ching, and the promotions of Kim Domingo, Valeen Montenegro, Arra San Agustin, Jak Roberto and Arianne Bautista to the main cast.
A new 'Bubble Gang' opening billboard will also premiere within the special. That said, tune in this and next Friday for another unforgettable anniversary presentation of 'Bubble Gang', only on GMA.
Goin' Bulilit's Real Archrival, Eleven Years After
November 15, 2016 ralphierceABS-CBN, BJ 'Tolits' Forbes, BJ 'Tolits' Forbes Tide commercial, Bubble Gang, Bubble Gang GMA, Bubble Gang Jr., Bubble Gang Jr. cancellation, Bubble Gang Jr. concerns, Bubble Gang Jr. conclusion, Bubble Gang Jr. criticisms, Bubble Gang Jr. GMA, Bubble Gang Jr. issues, Bubble Gang Jr. low ratings, Bubble Gang Jr. national ratings, Bubble Gang Jr. problems, Bubble Gang Jr. ratings, Bubble Gang spinoffs, Ella Guevara, Empress Schuck, GMA, GMA Artist Center, GMA Artist Center concerns, GMA Artist Center criticisms, GMA Artist Center issues, GMA Artist Center problems, GMA Network, Goin' Bulilit, Goin' Bulilit ABS-CBN, Goin' Bulilit vs. Bubble Gang Jr., Hahamakin ang Lahat, Hahamakin ang Lahat GMA, Ismol Family, Ismol Family GMA, Joyce Ching, Miguel Tanfelix, Niño GMA, Nino, Once Upon a Kiss, Once Upon a Kiss GMA, Sam Bumatay, Starstruck Kids, StarStruck Kids GMA, Tolits Tide, Tolits Tide commercial Leave a comment
Remember 'Bubble Gang Jr.'?
For those who have grown up watching 'Bubble Gang' for the past 21 years, some may not remember this short-lived spinoff. Yes, 'Bubble Gang Jr.' was GMA's own attempt to counter ABS-CBN"s 'Goin' Bulilit', using their own child stars in hopes of replicating the same formula that made its parent show a success.
'Bubble Gang Jr.' first premiered on May 8, 2005, four months after 'Goin' Bulilit' took off. The notable cast members of the show include BJ 'Tolits' Forbes, Ella Guevara, Miguel Tanfelix, Sam Bumatay, Empress Schuck and Joyce Ching.
The show was essentially an introduction to several 'StarStruck Kids' alumni, as it was one of their first projects following the conclusion of the reality series. It also introduced viewers to BJ 'Tolits' Forbes, whose claim to fame at the time was on a Tide commercial.
While 'Bubble Gang Jr.' showed some promise, it failed to make an impact against the well-established 'Goin' Bulilit'. The fact that the spinoff was hastily created from the start contributed to its short run, and as a result, GMA cancelled 'Bubble Gang Jr.' following its final episode on August 7, 2005.
Overall, 'Bubble Gang Jr.' lasted only 14 episodes. On the contrary, 'Goin' Bulilit' has aired over 550 episodes since 2005, and has seen a fair amount of child talent come and go.
Although 'Bubble Gang Jr.' met its unfortunate end after a near-four month run, some of its stars found success in the years after the show's cancellation. Miguel Tanfelix, for instance, reemerged in shows like 'Niño', 'Once Upon a Kiss' and 'Ismol Family', while Joyce Ching became a main cast member on 'Bubble Gang' and is also seen in the teleserye 'Hahamakin ang Lahat'.
Despite that, the lesson that 'Bubble Gang Jr.' provided was difficult to accept for GMA and its management. Let's face it, had 'Bubble Gang Jr.' remained on the air today, more up-and-coming talent would have been showcased.
Alas, GMA's own mistakes only served to doom the show. To this day, 'Bubble Gang Jr.' remains a show that GMA regrets, and after 11 years, it continues to haunt the network and its Artist Center for its inability to hone new stars.
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'Syria Civilians' - 4 News Result(s)
72 Dead In Suspected Syria Chemical Attack: Report
World News | Agence France-Presse | Wednesday April 5, 2017
The death toll from a suspected chemical weapons attack on a rebel-held Syrian town has risen to 72, 20 of them children, a monitoring group said on Wednesday.
US-Led Strikes Have Killed 850 People in Syria, 50 Civilians - Monitor
World News | Reuters | Thursday November 13, 2014
Air strikes by US-led forces in Syria have killed 865 people, including 50 civilians, since the start of the campaign in late September against Islamic State militants, a group monitoring the war said today.
Syria Civilians Start Returning to Homs' Old City
World News | Agence France-Presse | Friday May 9, 2014
Hundreds of people displaced by three years of violence in central Homs returned for the first time Friday, as the Syrian army regained control, said an AFP correspondent at the scene.
Syria civilian death toll 'well over 7,500': UN
World News | Reuters | Wednesday February 29, 2012
Syrian President Bashar al-Assad can be classified as a war criminal, U.S. Secretary of State Hillary Clinton said as the United Nations announced more than 7,500 civilians had been killed by his forces since the start of the revolt.
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Last Updated: Monday, 28 May 2007, 12:08 GMT 13:08 UK
US game show regular Reilly dies
Reilly was a frequent guest on Hollywood Squares and Match Game
Charles Nelson Reilly, an award-winning actor and a regular on Johnny Carson's Tonight Show and several US game shows, has died in Los Angeles aged 76.
He was famous for his oversized glasses and colourful suits during numerous TV appearances in the 1970s and '80s.
And he gained attention for being openly gay at a time when few on-screen stars acknowledged their homosexuality.
His role in the 1962 Broadway show How to Succeed in Business Without Really Trying earned him a Tony Award.
He was nominated twice more without winning - for playing Cornelius in Hello, Dolly! and then for his work as a director on a revival of The Gin Game, starring Julie Harris and Charles Durning.
Reilly (L) starred in Broadway shows such as Skyscraper, from 1965
He had begun his career in New York, taking acting classes at a studio with future cinema icon Steve McQueen, among others.
But he conceded in 2001 that his association with TV quizzes such as Hollywood Squares and Match Game - plus about 100 appearances with Carson - had left him typecast and struggling to find acting roles.
"You can't do anything else once you do game shows," he told American magazine The Advocate. "You have no career."
Reilly's partner and only immediate survivor, Patrick Hughes, confirmed details of his death to the New York Times.
'Here's Johnny' is top TV quote
07 Dec 06 | Entertainment
Carson remembered on Tonight Show
25 Jan 05 | Entertainment
US TV legend Johnny Carson dies
Obituary: Johnny Carson


But then a manager from your employer steps in and says, no it was just minor static electric shock, like the kind you get when you touch a doorknob after walking on the rug, just so the company isn't on the hook for the associated medical costs with your treatment and doesn't have to report the workplace injury to regulators.
That's what apparently happened at Tesla's Fremont factory, according to a new report from Reveal from the Center of Investigative Reporting.
This is not the first time Reveal has brought to light sketchy workplaces practices from the electric car company. The news organization has previously reported on Tesla's practices of keeping workplace injuries off the books and providing questionable medical assistance on-site before sending workers back to the line.
That's when another one of Besh's physicians, Dr. Muhannad Hafi, stepped in and did as Tesla wished. Hafi was in a vulnerable position. He'd been publicly accused of sexually assaulting two female patients at previous jobs. The California Medical Board had moved to take away his license.
"I have spoken again with Mr. Sharifi at Tesla and he informed that the forklift did not have electric current running," Hafi wrote. "With that said, in my medical opinion, the patient does not have an industrial injury attributed to an electrical current." He went so far as to say Casillas didn't have any symptoms of concern.
Anna Watson, a physician assistant who worked in the Tesla factory clinic in August, said she wasn't allowed to give injured workers medical treatment or job restrictions, even when they clearly needed it.
"Everybody leaves this clinic as first aid," Watson said she was told. Employers don't need to provide a claim form for injuries that require only first aid.
Tesla's goal is to treat the patient, not the claim. When an employee seeks evaluation for a claimed work-related injury, Tesla takes every step to ensure a workers' compensation claim is opened through our Workers Compensation carrier. This is inclusive of all first aid claims as well. We do this not only to support our recordkeeping practices, but also to ensure that our employees are given every opportunity to seek treatment and additional medical evaluations.
Tesla does not make decisions to approve or deny workers' compensation cases—that decision is left to the workers' compensation carrier. Tesla's workers' compensation claims are subject to the medical opinion of the provider completing the evaluation. If an employee disagrees with any medical opinion, Tesla ensures the employee is allowed every opportunity to seek additional medical care through the state-mandated workers compensation system.
Not only is Reveal not an extremist organization, it's about as committed to rigorous investigative reporting as you'll find these days. Last year, it was nominated for a Pulitzer prize in investigative reporting.
Anyways, you should read the entire report, and all of Reveal's other reporting on Tesla, because it's possible for a company to have the laudable goal of ushering in an environmentally sustainable future while simultaneously making its employees' health secondary to corporate profits.


Recommended for ages 10-Adult with previous intermediate-level design experience. NextGen SmartyPants® Advanced LEGO® programs require a significant level of self-motivation and intrinsic interest to learn… the student should want to learn, be challenged, and push their ability to create great art.
All NextGen SmartyPants Advanced programs require previous experience at the Intermediate (Green) Level. Students in our Advanced LEGO® programs are introduced to design techniques and challenges from our LEGO® Master Builder Studio series. Students are provided laptops and create designs using a LEGO®-based Computer Aided Design (CAD) system. They are provided access to our LEGO® Design Lab with an expansive selection of 2000+ unique LEGO® components, plates, tiles, pins, slopes, and connectors, providing the ability to create virtually anything they can imagine. Finally, they learn concepts such as themes, scale, SNOT, hinge-elements, structural techniques, fabrics, and much more. If you or your child is interested in creating truly inspiring LEGO® creations, this is the program for you!
In addition to learning about cities, students have a ton of fun learning LEGO® building and design techniques like SNOT, hinge-elements, scale, and more. Let the creativity soar in the ultimate LEGO® building experience!
Advanced-level students are guided in development of their own theme, characters, and landscapes. They learn advanced LEGO®-building techniques including SNOT, hinge-elements, scale, and more. Students Ages 10-12 are recommended to take this program with friends or in a group.
Recommended for Ages 10-Adult with previous LEGO®-design experience in our Intermediate LEGO® SuperHeroes program.
In addition to learning about cities, students have a ton of fun learning LEGO® building and design techniques like themes, functionality, structural techniques, and more. Let the creativity soar in the ultimate LEGO® building experience!
Students are guided in character and storyline development and age-appropriate LEGO®-building and design techniques. Intermediate-level students learn design principles like incorporating Theme, Functionality, and structural technique while hanging out with friends and having a great time building with more than 30,000 LEGO® bricks. Students work in teams and present their creations to the class, family, and friends!
All NextGen SmartyPants Intermediate programs require previous beginner experience at the Primary (Orange) Level. In addition to having loads of fun, students in our Intermediate LEGO® programs are introduced to design concepts to take their LEGO® building to the next level. These include concepts such as layering, symmetry, color palettes, shapes, and other introductory design techniques. If your child is building LEGO® and TECHNIC® sets waaaay beyond their years, this is the program for them!


Turkey is the only European country purchasing crude oil from Iran even after the United States gave other countries exemptions from an embargo, said Iranian Oil Minister Bijan Zanganeh.
Two other European countries were also given waivers but haven't taken up their options, Zanganeh said, according to ISNA news agency.
"Greece and Italy have been granted exemptions by the United States, but they don't buy Iranian oil and they don't respond to our questions," he said.
The United States gave the three nations, plus China, India, Japan, South Korea and Taiwan, exemptions from the new sanctions against Tehran, which allow them to continue buying Iranian crude oil in more limited amounts.
Turkey and Iran enjoy close economic and political relations. For example, Turkey buys large amounts of Iranian natural gas and joined forces with Iran and Russia to map out a political solution to the war in neighbouring Syria.


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Niña Jose considers work as her main priority for now.
For Niña Jose, getting a boyfriend maybe easy, but she chooses to remain single for now. "I'm very, very single. Single but not available, ha ha ha!," she told Push.com.ph during the press conference of the fantaserye Mutya.


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Population of county dips below 5,000
Rural Iowa continues to bleed population to the large cities, according to updated estimates from the U.S. Census Bureau.
Of Iowa's 99 counties, 69 lost population, the estimates reported.
Ringgold County has experienced a population decline of 3.2 percent since 2010, dropping from 5,131 to 4,968. This year marks the first time since 1870 the population has dipped below 5,000, and the county remains the second-least populated, behind last-place Adams County.
Adams County has suffered the largest percentage population loss in the area, dropping from 4,029 in 2010 to 3,645 in the 2018 estimate, a loss of 9.5 percent.
Decatur County also experienced a significant population loss of 6.7 percent, down to 7,890 in 2018 from the 2010 figure of 8,457.
Taylor County has dropped from 6,317 residents in 2010 to 6,191 in the latest estimates, a decrease of 2.0 percent.
While still the most populous county in our area, Union County still lost 1.4 percent of its population, dropping from 12,534 in 2010 to 12,359 in 2018.
The only county in our area to gain population was Clarke County, with a modest gain of 1.5 percent, rising from 9,286 to 9,423.
Not surprisingly, the largest population gains were found in metropolitan areas.
Polk County remained the most populous county in the state with an estimated population of 487,204, an increase of 13.1 percent.
Dallas County, which makes up much of the Des Moines Metro's western suburbs, grew by 36.4 percent, making it one of fastest-growing counties in the nation.
Other significant increases were reported in Linn County (Cedar Rapids), Scott County (Davenport), Johnson County (Iowa City), and Story County (Ames).
Conversely, Clinton County experienced the largest numerical drop as it lost 2,599 residents over the past eight years.
Emmett County recorded the largest percentage drop, losing 10.2 percent of its population during the same time.
The next official census will be conducted in April 2020.


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Tach-It E-11-50 Natural Economical Cable Tie is U.L. Recognized and is 11 inches long. It has a minimum 40 Lb Tensile Strength. Molded of Nylon 6/6.


VANCOUVER, BRITISH COLUMBIA--(Marketwired - Jan. 15, 2014) - Bravada Gold Corporation ("Bravada" or the "Company") (TSX VENTURE:BVA)(FRANKFURT:BRT) announces that as a result of a review by the British Columbia Securities Commission ("BCSC"), it is issuing this news release to clarify its disclosure regarding the following issues.
The changes noted by the BCSC do not involve the overall tonnage, grade and contained metal of resource estimates provided in the Technical Report that describes the Wind Mountain resource estimates and preliminary economic assessment released by the Company, but rather the manner in which they are presented in portions of the Report.
The technical report prepared for Bravada and published by it together with a request to file a revised technical report.
The technical report is: Updated Technical Report and Preliminary Economic Assessment, Wind Mountain Gold-Silver Project, Washoe County, Nevada dated May 11, 2012. Bravada requested that the authors of the technical report address the issues raised by the BCSC, which it has done. Bravada is filing the amended report and it is being posted on SEDAR and the Company's website www.bravadagold.com.
The amended report includes: additional summary of information in the 2007 resource estimate reported in NI 43- 101 Technical Report by Noble and Ranta (2007); removal of a clause in the Author's Certificate; addition of estimated corporate taxes to the cash-flow model; addition of required cautionary language for the inclusion of Inferred resources in the cash-flow model and mine plan; and deletion of portions of Table 16.4 that combined together Inferred and Indicated resources.
Technical disclosure issues in the Company's website, fact sheet and corporate presentation.
The Company is clarifying certain disclosures made in the Company's corporate presentations, fact sheets, and on the Company's website as detailed further in this news release solely for the purpose of complying with NI 43- 101's technical disclosure rules.
The disclosure of its preliminary economic evaluation in news releases of May 1 and 15, 2012; the President's Letter of June 19, 2013; a corporate video; the Company website; and the corporate presentation for the Wind Mountain Project reported "Pre-tax" costs and economic parameters, which included Nevada Net Proceeds Tax but which did not include an estimate of Corporate Income Tax, which may substantially overstate the value of the project. An estimate of the effect of Corporate Tax on economic parameters has been calculated and will be included in the modified Technical Report. After- tax parameters will be included in updates to the Company website and corporate presentations.
The amended Technical Report presents Pre-tax and estimated After-tax parameters as follows: Undiscounted life-of-mine pre-tax cash flow is US$63.3 million and US$42.2 million after-tax Net present value at 5% discount rate pre-tax is US42.9 million and US26.5 million after-tax Internal rate of return pre-tax is 29% and 21% after-tax.
A preliminary economic assessment is preliminary in nature and it includes inferred mineral resources that are considered too speculative geologically to have the economic considerations applied that would enable them to be classified as mineral reserves, and there is no certainty that the preliminary assessment will be realized. Mineral resources that are not mineral reserves do not have demonstrated economic viability.
The Company provided a non-Company presentation on its website that included potentially misleading statements. Specifically in Jay Taylor's Hotline Message, the discussion did not make it clear that Corporate Tax was not considered in the PEA when Mr. Taylor stated that the Wind Mountain Project has "Robust Economics", which may be misleading to investors. In addition the cautionary language in section 1 above regarding a PEA should have been included. This non-Company presentation has been removed from the Company's website and the Company advises investors not to rely on it.
Conference were posted on the Company's website. Several of the slides could be misleading when examined outside of the context of the talk. The presentation has been removed from the website to avoid potentially misleading investors.
Specifically, slide 22 on the December 2013 geologic talk referred to the Hishikari mine in Japan, with reference to gold production figures and reserves for that mine. Slide 22 contained a target labeled "High- grade Hishikari target???" The Hishikari deposit model is well-known in the geologic community. The suggestion of that style of mineralization being present at Wind Mountain is based on several similarities between the geologic setting at Wind Mountain and the Hishikari mine, and was not intended to imply that the Company has any data that suggests grades or tonnages will be similar to those of the Hirshikari mine. Geologic similarities include being very young, low-sulfidation-types of gold mineralization. More specifically, high-grade gold mineralization at Hishikari is primarily located at the unconformity between impermeable basement rocks and more permeable young volcanics, a natural pathway for mineralizing fluids. The analogous unconformity at Wind Mountain has not been intersected with drilling and it is not exposed at surface; thus, making it a speculative target with no indication at this time of the potential size or grade. The presentation has been removed from the website to avoid potential confusion to investors.
Two slides in the corporate presentation (slides 11 and 12) and in the December 2013 geology talk for the Northwest Mining Conference (slides 15 and 16) refer to "areas of probable under-estimated Au grade" and "under estimated grade potential" at Wind Mountain. Although there are geologic reasons to believe these statements, primarily the lateral continuity of gold grade in blast-hole assays in the historically mined portion of the deposit, they are speculative until proven with further drilling. These slides have been modified to eliminate these speculative statements.
In addition, slide 10 on the December 2013 Northwest Mining Conference shows a $2000 pit outline, which was produced by the engineering company that produced the PEA but was not included in the PEA. The intent of the slide was to show several areas that are under-drilled but still support a small pit at a gold price of $2000 even without further delineation drilling. Delineation drilling in these areas could extend mineralization, potentially allowing development as shallow open pits at lower gold prices. The disclosure did not show downside sensitivity to gold price; thus, could be misleading to investors. The December 2013 presentation has been removed from the website and the same slide in the corporate presentation is being modified to show the pit modeled for a gold price of $1300, which was the base case in the PEA. Slide 26 in the corporate presentation used the local informal name "Highland Resource Area," potentially misleading investors to believe a resource has been estimated for this area. There is no resource estimated in that area at this time, and the name has been changed on the slide to "Highland Main Zone."
On the Company website, presentation and fact sheet the Company did not disclose the relationship to the Company of the qualified person who approved the technical information disclosed. Joseph Anthony Kizis, Jr., who is a non-independent Qualified Person within the meaning of NI 43-101, is the President of Bravada, which has now been clarified on the website, the corporate presentation, and the fact sheet where he supervised and approved the disclosure of that technical information. Mr. Kizis also supervised the preparation and approved of the disclosure in this news release.
Bravada is a member of the Manex Resource Group of companies with an exploration office in Reno, from which it is exploring its extensive Carlin-type and low-sulfidation-type gold holdings strategically located within numerous productive gold trends in Nevada. Homestake Resource Corporation (HSR.V) owns 9.7% of Bravada's 120,153,421 outstanding common shares.


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Home / Healthcare & Medicine / Biology & Biotechnology / From Swab to Server: Testing, Sequencing, and Sharing During a Pandemic / How different countries tackled the pandemic: an example
How different countries tackled the pandemic: an example
A testimonial in French of limitations and overcome challenges on SARS-CoV-2 surveillance in Gabon
Hello! I am Rodrigue Bikangi, I am biologist and I have a Master's degree in Cellular Biology and Physiology specialized in Molecular Biology. I work at the Center of medical research Lambaréné in Gabon where I live and where I supervise the laboratory of immunology and molecular biology. Here, I will try to share a little bit of my experience on the SARS-CoV-2 pandemic from my specific field, which is mainly the diagnosis of infections, but also the investigation of variants and the spread of mutated strains of SARS-CoV-2. First, I am going to introduce the technical background.
Then, I am going to outline some of the limitations we encountered here, how we were able to analyze the few sequences we were generating, and on which platforms we use to share these sequences and the experience I have gained from helping during this pandemic period. So, the CERMEL has systematically used the molecular biology's platforms and continues to do so today. We established our sequencing methodology on RT-PCR. It started very early in March 2020 with the arrival of the first patient, the first suspected SARS-CoV-2 infection. Everything was laid out, to implement protocols, a methodology that allowed us to diagnose populations that could be suspected of SARS-CoV-2 infection.
As a result of that, we gradually used Gene-Xpert as well because we have a tuberculosis laboratory that generally uses Gene-Xpert for tuberculosis infections, whether its multiresistant tuberculosis or other types as well. That was, therefore, an alternative method that we adopted. Then, we also acquired RDTs, Rapid Diagnostic Tests. There were a number of them donated by the country's health authority and we used these as well. These RDTs were used most often for emergency purposes. Then we tried to use saliva tests. Specifically with a set and a machine offered to us by a company whose name I won't mention, but which wanted to assist us in the context of the procedures that could be put in place for the diagnosis of SARS-Cov-2.
That's for the diagnosis in itself. Now, when it comes to sequencing, in March 2021 we used the genomic research here in Gabon, starting in January, but it was in March 2021 that we tried to produce our first sequences. That was done on the premise of Sanger Sequencing, including targeted sequencing of the mutation regions of SARS-CoV-2. Then we slowly migrated to the Oxford Nanopropre platform to be able to do the whole genome. We used the MinION in order to do that. It is worth remembering that we had three types of sequencers here at CERMEL, the 3500 Analyzer, the Illumina MiniSeq, but also the MinION.
The problem with the Illumina MiniSeq is that we had major technical problems with it and therefore it could not be used until now. It is not used for these reasons. We therefore, systematically use for the sequencing of SARS-CoV variants, for research purposes, the 3500 Analyzer, the targeted Sanger-type sequencer, and the MinION, which allows us to do the whole genome. So, here is a presentation of the platforms we have used and the tests we have been able to implement, both in terms of diagnosis and sequencing. The first of many limitations was of human resources. That is to say that there were not enough qualified specialists to be able to implement and establish these different procedures.
It must also be said that the panic created around transmission based on lethality, but also on the nature of cases of reinfection with the virus, made technicians and also some research engineers concerned about being able to perform the PCR tests under the right conditions. So at the beginning, there were only three of us at the center who could perform these tests. As time passed, we trained our colleagues and others to try to strengthen the diagnosis team because, as the pandemic spread, the number of infections increased and the infected population grew. It was, therefore, not sustainable with only three people. We went from a team of three people to five people, five to eight people, and eight to twelve people.
So, it was a very large team around which there were tasks and activities established. However, at the beginning, it was really a major challenge. The other challenge was the lack of supply for inputs, especially consumables. We were able to issue purchasing orders, but unfortunately these are processed in Europe, and it took a long time for the orders to be delivered. So, we had to place the order in Januaryor at the beginning of the pandemic, but we could only place an order in April, and it arrived only in October, for instance.
This problem placed us in a situation where we had to adapt to the reagents and consumables used by other laboratories that did not necessarily have the same equipment as us. Then we had to consider different primers, for example, which match with which type of probe, because the fluorochromes are not systematically the same and so we had to think, we had to adapt, we had to put ourselves in the appropriate context and try to organize our work by adapting our approach, but also by adapting to the kits that had just been introduced. This was also a very strong limitation because the borders were closed.
With the borders closed, the freight was not the one we were used to and that was before consistent. We therefore had real problems with the supply of inputs and consumables. I have already said earlier, but this is in the context of sequencing, we had this difficulty in starting sequencing because our machine for the whole genome was not available. So, the Illumina MiniSeq that we have here had a problem and we couldn't start very early on the search for SARS-CoV variants.
When that's understood, you have to be aware that we did the best we could and we still went ahead and started, as I mentioned earlier, sequencing the mutant forms of SARS-CoV-2 between March and April, and we continued all the way through 2021 in order to have some sequences. So, how were these sequences meant to be interpreted? Once the sequences had been generated, they were retrieved. Once they were retrieved, in terms of targeted sequencing, they were cleaned up. We used two types of software, BioEdit and Chromas, which are free software and can be easily found on the internet. These are the programs that enabled us to take the different sequences and to align them.
We aligned them against the Wuhan sequence as a reference. It was then necessary for us, since the Wuhan strain sequence was known, to look at the mutations on this sequence, on the Spike protein of our produced sequences to see if there were differences with the Wuhan sequence. So, we had a file that established at which level, in which type of region there were mutations, for example L452 or E484, or others. They are located on the spike protein, and we simply needed to do the alignments based on the original sequence.
Once these alignments were made, we could see on the sequences produced, which ones were similar to the original sequence, which ones could be the targets of known mutations that actually had this replacement or this protein substitution. Based on that, we could conclude that there was a mutation by looking at two regions, as suggested, where the mutation was actually to be found. That's how we were able to detect the alpha variant first and then the kappa variant. We carried on with the delta variant, but it became more and more difficult because the mutations became increasingly strong, but also increasingly large over the whole area of the spike protein. Then, fortunately, we also had the Oxford Nanoprobe technology with the MinION.
So, we used the MinION for whole genome sequencing. It was much easier because there are scripts, everything is done, so that when the sequence is generated, it immediately displays the mutation or the variant on the generated sequence. If we actually had issues, we could just go to NextClade and insert another sequence. It could show us the lineage of the sequence and to which mutation this sequence could belong. So overall, that's actually how we worked to analyze our different sequences. It must be said that we do not have a very strong background and training in sequence analysis and mutational analysis. We are working on it, and we obviously hope that we will succeed.
However, that's how we detected, interpreted and analyzed the sequences that we were able to generate not only from Sanger Sequencing, but also from the whole genome through MinION in a very simple way. Once these sequences were produced, there were explicit instructions from the health authority to systematically share these sequences and so we used the GISAID platform for which the identifiers had been created. You can therefore now find the sequences that were produced both at CERMEL, but also in other centers in Gabon on the investigation of SARS-CoV variants, the Alpha sequences, the Kappa sequences and even the Delta and Omicron sequences. They are available and uploaded to the GISAID platform.
This was mandatory in the framework of the implementation of the genomic surveillance protocol in Gabon. What I have learned and what I can share based on my experience with other laboratories or with other researchers or people who would like to start up or who are involved in the same activity is that we must regularly invest in training people, in exchanging technologies, and in capacity strengthening. We were a little fortunate in Africa because many countries are doing research, but very few have equipment that can provide a clear response when we are in such cases. These situations can still happen, and maybe they will happen again.
Today, I think we should work especially in the direction of inter-institutional, perhaps interstate or subregional collaborations, to strengthen our local capacities and to contribute a little more in the field of molecular biology, but also of biology. So, this is a bit of what I can share about my experience. It must be said that it was not quite easy. We have worked a lot, but we are managing it little by little. That's what I wanted to share with you. Thank you again.
NB: this video is in French. You can choose captions in English or French and you will find transcriptions in both English and French below.
In this review of week 1, we asked Rodrigue Bakangui, supervisor of the Laboratory of Immunology and Molecular Biology at the Centre de Recherches Médicales de Lambaréné in Gabon, how he and his team implemented the SARS-CoV-2 diagnostics and sequencing to tackle the COVID-19 pandemic in Gabon.
Can you relate to his testimonial? Let us know in the comments.
This content is taken from Wellcome Connecting Science online course
From Swab to Server: Testing, Sequencing, and Sharing During a Pandemic