Yes! It is confirmed! Committed! And a spin in the trend! The big 1000 crore movie has got a name and it's "Thugs of Hindostan." For which, Big B and our Mr. Perfectionist (we call him Amir!) are going to share the screen for the first time and the movie will appear by Diwali of 2018. Now, you must be amazed to read such far away prediction but when two legends appear on the grounds for the first time, the news is never too early or late!
The two actors are working on Yash Raj Film's project "Thugs of Hindostan," and it will be made under the direction of Vijay Krishna Acharya, whose previous works are Dhoom3 with Amir khan.
Though the leading actress is still not announced, but we are just can't wait to spot that adorable face.
Earlier, we hardly heard anything from Aamir khan regarding the project, but now he has just reassured all of us on Twitter. Feeling his childlike joy, he shared these words, "Finally, the moment I've been waiting for has come…to work with my icon, Mr. Bachchan! Thanks Adi, Victor, for 'Thugs of Hindostan'.
Not only him, our "Hero", Jackie Shroff has also been approached for the "Thugs of Hindostan." Sources say, he has quite a crucial role in the movie.
With these leading players and even admirable supporting cast actors, this project is surely not less worth than a thousand crore!
This eminent and distinguished movie project will be produced by Yash Raj Films (YRF) and now, we are just waiting to see the leading lady's pretty face of the movie!


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ACCEPTED

#### According to
Index Fungorum

#### Published in
null

#### Original name
Ceratostomella exigua Hedgc.

### Remarks
null

redpajama_set_name: RedPajamaGithub


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Pfizer and Bristol-Myers Squibb's next-gen anticoagulant Eliquis has been stealing warfarin market share for years, but that doesn't mean it's slowing down. The drug now has new data showing it's the safer option for a tough-to-treat group of atrial fibrillation patients.
Presented at the American College of Cardiology annual meeting Sunday, the data showed that adding Eliquis to P2Y12 antiplatelet therapy—such as Sanofi's Plavix or its generics—with or without aspirin, cut the number of serious bleeding episodes compared with warfarin combination treatment. In the study's Eliquis arm, 10.5% of patients had suffered major or clinically relevant bleeding episodes at the six-month mark, compared with 14.7% of patients in the warfarin arm.
Separately from Eliquis' performance, the study, dubbed Augustus, also showed that patients receiving aspirin alongside their antiplatelet and anticoagulant therapies saw an 89% increased risk of bleeding compared with patients who didn't use aspirin. Those results "provide really important information for physicians as they consider treating these high-risk patients," DeMicco said.


text:
Find a flight from Manchester to Munich
Journey duration 1 h 57 min
Get from Manchester to Munich with Virail
Planning to travel from Manchester to Munich? Sometimes it can be hard to know where to start - but that's where Virail comes in. Our powerful search engine will explore all the options available for your trip, combing through more than 2000 transport companies all over the globe. All you have to do is enter your travel dates. In an instant, we'll show you a thorough list of possible routes, with all the information you need to make your decision. Choose from buses, trains, and more, connecting or direct, one-way or round trip - whatever you prefer. When you see your results, select your chosen option and Virail will redirect you to the provider's website, where you can complete your booking. Let us do the legwork for you, and you can just sit back and enjoy your trip.
How can I find the cheapest plane tickets to get from Manchester to Munich?
Prices will vary when you travel from Manchester to Munich. On average, though, you'll pay about £ 354.77 for a plane ticket. You can find plane tickets for prices as low as £ 27.21, but it may require some flexibility with your travel plans. If you're looking for a low price, you may need to prepare to spend more time in transit. You can also often find cheaper plane tickets at particular times of day, or on certain days of the week. Of course, ticket prices often change during the year, too; expect to pay more in peak season. For the lowest prices, it's usually best to make your reservation in advance. Be careful, though, as many airlines do not offer refunds or exchanges on their cheapest plane tickets.
Flights from Manchester to Munich
EasyJet 18:00 – 21:00 Manchester – Franz Josef Strauss £ 27.21 2 h Direct
Lufthansa 17:40 – 20:35 Manchester – Franz Josef Strauss £ 172.31 1 h 55 min Direct
How much time will it take to go from Manchester to Munich by plane?
The journey between Manchester and Munich runs for about 705.9 mi and usually takes approximately 11 h 58 min. However, this figure is an average and will vary according to a number of factors. Depending on the method of transport that you choose, you might be able to arrive in less time. The shortest time in which you can complete this journey is 1 h 55 min. If you are trying to get from Manchester to Munich as quickly as possible, look for express routes or non-stopping services. Direct routes are not always the fastest way to travel. At times, you may even find a quicker route that involves a connection in another city.
When do departures from Manchester to Munich leave?
In general, the first departure of the day will leave Manchester at 07:00, and the last departure will leave at 18:00. However, it is important to remember that this is general information, and these journey times are not necessarily available every day. Timetables for each provider on the route from Manchester to Munich can change from day to day or month to month. In particular, many providers offer different schedules on weekdays and weekends. Public holidays, local events, and seasonal timetables can also cause schedules to change.
What are the airports on the Manchester to Munich route?
You can travel from to using Virail's search engine to find your ideal journey from all the available options. In , you can use airports such as Mánchester and Manchester. These will take you to airports like Aeropuerto Internacional De Múnich-Franz Josef Strauss and Franz Josef Strauss in . However, it is always important to look at the details when planning an international journey. There may not be a direct connection between your airport in and . If this is the case, then you will have to travel by using a connecting journey, changing at one or more airports along the way.
You can depart from Manchester at airports such as Mánchester and Manchester. At the end of the journey, you will be able to arrive in Munich at the following airports: Aeropuerto Internacional De Múnich-Franz Josef Strauss and Franz Josef Strauss. Some of these airports may only be served by particular airlines. Because of this, there may not be a direct connection between all the airports in Manchester and Munich. Plan your journey carefully, as it may be necessary to make another connection before departure or after arrival.
How many daily journeys are there from Manchester to Munich?
If you want to get from Manchester to Munich, you can travel with providers such as Lufthansa, EasyJet, , KLM, Air France, Eurowings, Aer Lingus, British Airways, Austrian Airlines, Finnair, TAP AIR PORTUGAL, SAS, Ryanair, Etihad Airways, Emirates, Iberia, Aegean Airlines and Oman Air. You will find a maximum number of 111 departures per day. On average, there are 21.5 departures per day. Book in advance to make sure that your journey will run smoothly and you depart at a time that suits you best. When you travel, you can look for direct journeys or other routes which offer connections and changes. On average, there are 1.5 direct journeys between Manchester and Munich. If you don't mind changing along the way, you may find more options available to you. There are usually 20 journeys with at least one change. These numbers may vary depending on the time of year or day of the week. Some travelers prefer direct routes, particularly if they are carrying heavy suitcases or concerned about missing a connection. Others enjoy the flexibility offered by connecting journeys. Whatever you prefer, Virail can help you find the right options to suit you.
Flights United Kingdom - Germany »
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Main routes to Munich
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Destination airports
Aeropuerto Internacional De Múnich-Franz Josef Strauss
Franz Josef Strauss

redpajama_set_name: RedPajamaCommonCrawl


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Once you click the Submit button, your deal submission is now pending for Chamber staff approval. Please allow at least one business day for the approval process. No notification will be sent - please check the website to see your posted deal. Once approved, you can view your deal at Member To Member Discounts. Be sure to promote your deal using social media and tag us for a repost. To edit your deal, please resubmit the application and indicate "EDIT" prior to the new deal description in the Discount Offer text box. Deals will be deleted according to expiration date. To request a deal be deleted, please contact Jamie Strom. There is a maximum of five deal postings per company at a time.
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The boom in 'Waldorf Dolls' raises questions about the significance of these types of dolls. Philipp Reubke, senior Waldorf kindergarten teacher and member of the International Association of Waldorf Kindergartens (IASWECE) asks, 'what is a Waldorf Doll'? Is there a 'real' Waldorf Doll?
Waldorf dolls can be bought in many larger Waldorf schools, in some toy stores, and of course on the internet. There are even a number of manufacturers that have applied for "Waldorf doll" as a brand name. But are these commercially available dolls actually the real, original Waldorf doll?
Rudolf Steiner often spoke about the special qualities and needs of the young child, as well as the inner attitude and soul qualities that the adult needs to acquire in order to support the development of the child. However, if one is looking for a detailed description of how a Waldorf kindergarten should look like and the concrete activities that should take place there, these are not to be found in Steiner's work. No program and no recipes!
There are, however, a few exceptions, a few details that Steiner describes quite concretely. And the doll is one of these. One can simply look up what the original Waldorf doll was to look like.


The kratom community online has been under fire from several directions recently. A number of dubious anonymous and hired sources of disinformation, impossible to vett, have been thrown into the conversation surrounding the question, "What the hell is going on with imports?"
Some vendors are having no problem receiving their shipments, while others -- who think they are equally diligent at following the FDA's published rules -- are having shipments held up on seemingly political or other procedural grounds.
Could The Troubled Vendors Be Missing Some Key Fact?
One factor in the FDA's displeasure at seeing so much kratom arriving in the United States, was mentioned plainly in the most recent FDA update of Import Alert 54-15. It noted that the FDA is now watching Facebook for evidence of which importers are selling kratom for human consumption, a use of the herb that the FDA has not yet approved.
Paying the Price of Freedom.
I would like to see an old custom in our country brought back: The freedom to take whatever you want into your body, so long as you're prepared to be responsible for the consequences. No more whining to the state or federal government that someone poisoned you or gave you a tummy ache. This puts the responsibility for doing some accurate research back on us. It's a big task, but I do it all the time now and my health is better than those who let the average MD do their research for them.
Obviously, businesses selling Kratom must guarantee that their product is not adulterated with other substances, or contaminated with microbial organisms. That might give the FDA something legitimate to do, or it could be guaranteed by private testing agencies.
This practice of self-responsibility -- if it was brought back -- would bring about big changes in American society. I think it would be a very good thing.
Read more: Why Does the U.S. Allow Medical Banditry?


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« Congaree National Park Adds New Wilderness | Main | BLM Decision Keeps Dirty Fuels in the Ground »
Marine Sanctuaries and the Future of Our Ocean
Johnston Atoll National Wildlife (courtesy Jim Maragos/USFWS)
On Tuesday, President Obama announced his proposal to expand a marine sanctuary in the Pacific Ocean, a move that could double all of the world's protected ocean.
By using his executive authority to add protected areas to the Pacific Remote Islands Marine National Monument, the President intends to create the world's largest marine sanctuary in a threatened expanse of the south-central Pacific Ocean. According to The Washington Post, the existing protected areas in the monument may be enlarged by nearly 700,000 square miles. The plan will not be finalized before the White House consults with outside groups, including environmentalists and the fishing industry.
"The Pacific Remote Islands Monument is an important part of the most widespread collection of marine life on the planet under a single country's jurisdiction," describes the National Oceanic and Atmospheric Administration (NOAA). The monument includes seven small islands controlled by the U.S. and some of the most diverse coral reefs in the central Pacific. Protections would extend 200 miles offshore of each of the islands, blocking fishing and other tampering activities in these pristine ecosystems.
Many endangered species of fish, crustaceans, turtles, and marine mammals in need of protection exist in this unique ecosystem. It is only the latest of the President's efforts to safeguard natural environments, though his first major step for marine conservation.
The President's announcement follows a recent decision by NOAA to reopen the process for Americans to nominate new marine sanctuaries. That decision opens the door for the public to protect places and preserve the health of marine communities in the continental U.S. Presidential advisor John Podesta announced the new policy on June 10th.
"Our national marine sanctuaries not only protect special places... but they promote responsible and sustainable ocean uses to protect the health of our oceans for future generations," said NOAA administrator Kathryn Sullivan, Ph.D.
This rule is a momentous step forward in the efforts to preserve America's enduring natural history. It will help propel forward several important marine sanctuary proposals such as the Chumash Heritage National Marine Sanctuary, where the Sierra Club Santa Lucia Chapter has worked with the Northern Chumash Tribal Council and other grassroots organizations toward nominating a new National Marine Sanctuary. Maintaining these spaces is vital to the continuation of the region's historical and cultural legacy.
The State Department also held the "Our Ocean" Conference this week. The conference drew international science, policy, business, and environmental leaders to Washington D.C. to discuss future collaborative efforts to protect the ocean.
"The ocean fuels our trade, it provides much of the food we eat, the air we breathe. It is home to vast, extraordinary ecosystems… but today this incredible resource is threatened by unsustainable fishing, pollution, climate change," announced Secretary of State John Kerry about the conference's intentions. "There can be no doubt that the ocean requires our protection, and our collective action."
These efforts indicate Obama's willingness to give due attention to critical environmental priorities. Along with this marine protection, the President has invoked executive orders 11 times to protect American lands. In May, the President designated the Organ Mountains-Desert Peaks as a National Monument. He cited the importance of preserving natural spaces for our cultural and natural history as a driving factor in this action.
These new safeguards and promises are building Obama's renown as an environmental champion. His administration's recent actions also indicate his respect for the public's demand for responsible environmental policy.
However, there is still much work to be done.
At the "Our Ocean" Conference, U.S. Trade Representative Michael Froman made vows to work on several key policy issues related to fishing regulation.
"We are also seeking, in [the Trans-Pacific Partnership (TPP)], ground-breaking commitments to protect our oceans – commitments never seen before in a trade agreement," Froman said at the conference, mentioning efforts to advance sustainable fisheries management and combat illegal fishing.
However promising these statements may be, they don't seem to match a leaked draft of the TPP's environment chapter from earlier this year that reveals weak language on trade in illegally harvested timber, wildlife, and fish. We hope that Froman and the Obama administration will make good on their verbal pledges and work to strengthen this language. Making fair trade deals to protect the environment, including species of fish and other wildlife, will continue to build President Obama's environmental legacy.
"This [Organ Mountains-Desert Peaks] designation will be a recognition that, like the cultural and historic sites already protected by President Obama, our country's wide open spaces and large natural areas are an important part of the American story," said Sierra Club Director Michael Brune in May. Both on-shore and off, special places and threatened wildlife still need the protection that federal action can provide. Given the President's momentum, this great work is just the jumping-off point.
-- Stephanie Steinbrecher
Posted by Sierra Club at 01:28:02 PM in water, wildlife
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Renting an unfinished building that was meant to be a row of shops and converting it into the first Child Africa School was not without it's challenges.
Word spread fast across the mountains, far and wide that this school was to be for poor and disadvantaged children, whose families could not afford education.
Local communities in and around Kabale watched the transformation with eagerness, in the hope their child would qualify for a place.
The founder and managing director Julie Soberg held meetings with parents / guardians in and around Kabale.
Making sure the communities received the correct information was important.
When the school opened, the idea was to enrol 70 pupils.


Instead of using text to explain 'How-to' tasks, representing them step-by-step is much better as your employees will get it right without having to read much. Training with simulations does exactly that.
Without disturbing the functionality of the newly implemented Enterprise Resource Planning (ERP) system or any other software for that matter, you can train employees to work on it effectively, using simulations. In simulations, you mirror the real-world environment to train your employees on how to use the system. It will avoid serious consequences of experimenting on the actual workflow of the installed ERP.
This blog discusses the importance of Watch-Try-Do simulations and how to use this approach for software training.
You can design simulations for software or ERP end-user training in the simplest format, which is Watch-Try-Do. The training community also calls this the 'Show-Try-Test' method. Generally, software training extends online from the classroom. You will explain the features and benefits of the ERP to employees in the instructor-led training program. Then you move your training platform online for practice exercises. This can be done effectively through simulations.
In the WATCH/SHOW phase, you can show how the employee needs to work on the software. For this, we first need to record the step-by-step actions that need to be performed. The steps in operating the software are demonstrated to employees as a video or through an online course.
For example, if you want to train employees on generating an invoice, placing an order, or opening Outlook and sending an e-mail, you can show the steps of how to do them in the watch or show mode using videos and flow diagrams.
Employees are given a chance to try executing the demonstrated steps in the TRY mode. They will get hint options and highlights on the screen to help them. This acts as reinforcement and facilitates learning by doing.
Kinesthetic learners like this very much as they love to learn by doing. Even if they miss the order of the steps or forget in between, hint options and highlights come to their rescue. In a way, this engages employees in learning the procedure.
In the DO mode, employees execute the steps in the process on their own, without any hints. . If someone gets stuck at any point, the online Help option will be available.
Practice makes perfect. Your employees can view the simulation videos and try any number of times till they gain the confidence to perform the procedure on the ERP. They can always go back and view the whole procedure for reinforcement. Your employees will have online help manuals and support systems handy to access and apply them in their daily work.
The good thing here is, you can customize WTD simulations as per the nature of work, roles, and responsibilities. You can create precise, role-based simulations for employees, supervisors, managers, administrators, and more. By this, employees are trained on what they are supposed to do and to the extent required. The result is, your employees work well on the newly implemented software. In this way, your organization utilizes the software to the optimum extent and improves efficiencies.
Want to know how e-learning can support your corporate training? Check our free E-book: Practical Guide on Custom E-learning: A Handbook for Managers.


A letter of instruction is meant to help guide your representatives through administering your estate after your death. Imagine that you could stand over the shoulders of your spouse or children or other loved ones and tell them exactly what needs to be done. Very likely, these individuals are going through a tremendous amount of stress and grieving. But you can ease their burden by leaving step-by-step instructions to help them through the process. That is the purpose of a letter of instruction.
There is no right or wrong way to prepare a letter of instruction. A few ideas were mentioned above. Include a list of where your assets are located, how to access your accounts, where you keep important title documents, etc. These pieces of information are essential to managing any estate.
However, your letter can provide even greater detail. What subscriptions (cable, newspaper, lawn service) need to be canceled? What are the names and phone numbers of your advisers (financial, medical, spiritual, insurance) that should be contacted after your death? How can your representatives contact your family to let them know of your death? Where would you like your funeral held? Do you have a preferred program for your funeral? Do you already have an obituary prepared?
Your letter can also include instructions for distributing small items of personal property that have low market value — but high sentimental value — to your family. You may wish to distribute these items to certain beneficiaries rather than having them sold with your other property as part of an estate sale. Letting your representatives know about your wishes could keep these items from being lost forever. Remember, however, that your letter of instruction can only voice a preference for how you want untitled personal property distributed. All gifts of titled property must be made through proper legal documents.
Talk to an attorney about a letter of instruction.
Estate planning involves more than just legal papers. It is also about more than just your own well-being; it is about providing for your loved ones after you are gone. To discuss how you can take care of your loved ones after your death, contact the Oklahoma City estate planning attorneys at Postic & Bates for a free, no-obligation consultation appointment.
Newer PostWhat is an Advance Directive for Health Care?
Older PostDo I Need Probate to Get My Inheritance?


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We retrospectively analysed 58 acute leukaemia
Posted on November 30, 2018 by plas9821
We retrospectively analysed 58 acute leukaemia learn more patients who had IA during neutropenic period after chemotherapy and whose serum GM was serially monitored until discharge or death. The kappa correlation coefficient was used to determine the strength of correlation between GM and clinical outcome (survival or death) of IA. The correlation between clinical outcome and GM kinetics was good at week 6 [κ = 0.663, 95% confidence interval (CI): 0.465–0.861] and excellent at week 12 (κ = 0.819, 95% CI: 0.667–0.91). Survival was significantly better in patients whose GM values normalised than in patients with persistently
positive GM (P < 0.0001) regardless of whether neutropenia resolved or acute leukaemia responded to chemotherapy. In neutropenic patients with acute leukaemia, selleck products serum GM correlated strongly with survival outcome of IA. This finding further supports the usefulness of the GM index as a surrogate marker for assessing IA outcome and the need for serial GM testing in therapeutic monitoring. "
"Cryptococcus neoformans is a medically important fungus and can infect all the organs of the body. As vascular endothelial
cell is an important target for C. neoformans to penetrate any organs, the differential protein expression of human umbilical vascular endothelial cell (HUVEC) after incubating with C. neoformans may be the key to penetration. The proteins of HUVECs incubated with C. neoformans and normal HUVECs were collected and purified. After two-dimensional electrophoresis, the differential protein expression was identified by matrix-assisted laser desorption/ionisation mass spectrometry. The mRNA levels of some proteins were measured
by real-time PCR. Three proteins were found significantly overexpressed in HUVECs incubated with C. neoformans, and nine other proteins were downregulated. The mRNA Metalloexopeptidase levels of S100A10 and peroxiredoxin I fluctuated with the protein levels. These results suggested that the expressions of peroxiredoxin I and S100A10 were regulated during the process of invasion of HUVECs by C. neoformans. We hypothesise that these proteins take part in the modifications of HUVEC cytoskeleton and the tolerance to oxidative stress, which may affect the process of invasion by C. neoformans. "
"Combination treatment of paediatric invasive fungal infections (IFIs) has rarely been reported. A total of 17 children with 19 IFI episodes were enrolled in the study. The median age of the patients was 5.3 (range 0.5–17) years. IFI was classified as proven in 4, probable in 12 and possible in 3 episodes. These patients received empiric antifungal treatment, which consisted of liposomal amphotericin B (LAmB) monotherapy for a median duration of 12 days (range 3–69 days). All patients were refractory to LAmB; therefore, caspofungin was added to the therapy in 11 patients. In the remaining six patients, LAmB was ceased and a combination of caspofungin and voriconazole was started.
Mannan C albicans serotype B-specific sera antibodies levels ind
Mannan C. albicans serotype B-specific sera antibodies levels induced by immunization find more with M5-BSA conjugate did no correlate with specific antibody-secreting cells counts. Alteration of mannan C. albicans serotype A-specific IgM and IgG antibody production induced by immunization with M6-BSA conjugate distinctively revealed
IgM/IgG isotype switch (Fig. 4). Purified cell wall mannan does not always maintain their native conformation. To maintain mannan native conformation intact for the analysis of antibodies generated during the immunization with conjugates, we used intact yeast and hyphal cells of C. albicans serotype A in whole cells ELISA assays to determine natural cell wall mannan-specific antibodies levels (Fig. 5). We observed higher yeast and hyphae-specific IgM sera levels following M5-BSA immunization in comparison with control although without significant alteration throughout immunization. M5-BSA conjugate immunization induced significantly higher
levels of yeast and hyphae-specific IgG antibody levels in comparison with IgG levels in sera of controls only after the primary sc injection of conjugate (Fig. 5). IgG levels induced by subsequent M5-BSA conjugate injections were comparable or lower than IgG levels Decitabine chemical structure in sera of controls for both morphological forms of C. albicans serotype A. Yeast and hyphae-specific IgA levels significantly increased after primary M5-BSA conjugate injection and decreased after the primary sc booster administration to the levels comparable with IgA levels in sera of controls (Fig. 5). For M6-BSA conjugate and whole cell–specific IgM levels, we obtained similar results as for M5-BSA conjugate immunization. PAK6 Hyphae-specific IgM levels in immune sera were slightly higher than or comparable with control (yeast form, secondary ip booster injection, 3rd ip) but without significant
alteration throughout immunization (Fig. 5). Immunization with M6-BSA conjugate induced C. albicans serotype A yeast form specific IgG levels comparable with yeast that form specific IgG levels in sera of controls. For hyphal form of C. albicans serotype A, primary sc booster injection and secondary booster injections (both routes of administration, 3rd ip and 3rd sc) induced significantly higher IgG levels in comparison with sera of controls with maximal peak after secondary ip booster injection (Fig. 5). Only for hyphal form of C. albicans, whole cell–specific IgA levels significantly increased after primary M6-BSA conjugate injection. The alterations in C. albicans serotype A whole cell–specific IgG levels after individual administrations of conjugates reveal differences between conjugates. Different changes of the whole cell–specific IgG levels in the course of immunization with conjugates indicated different specificity of induced IgG antibodies and indicate M6-BSA conjugate as preferable for induction of potentially opsonizing antibodies.
Recent reports have shown that RP105-deficient B cells are defect
Recent reports have shown that RP105-deficient B cells are defective in their response to TLR2 and TLR4 ligands, whereas it is likely that RP105/MD-1 positively regulate TLR2/TLR4 responses in B cells.39 In contrast, Divanovic et al.40 reported that RP105 negatively regulates LPS-induced responses in macrophages and dendritic cells.
In the present selleck compound study, we examined RP105 to ascertain the expression of innate immune-related molecules in B cells. The major population of peritoneal B cells has been well reported to be B-1a cells and the immune function of this subset is essentially different from that of the conventional B-cell subset (B-2 cells) that exists in other organs. The present results obtained by flow cytometry suggest that the major population of intestine-related B cells (MLNs, PPs, colon lamina propria) has a B-2 lineage. Next, we examined the production of IL-10 and TGF-β1 in TLR-mediated B see more cells. Mononuclear cells were isolated from several
parts of BALB/c mice and magnetically purified using microbeads. Next, purified B cells (B220+ PDCA-1−) were cultured with or without TLR ligands, then cytokine concentrations in the culture supernatants were measured by EIA. The B-cell fractions used in the experiments were confirmed to be > 95% pure by flow cytometry (Fig. 2a). Although IL-10 production was induced in TLR ligand-mediated B cells, the level of production in CpG-DNA-stimulated cells was significantly higher than that in LPS-stimulated cells (Fig. 2b). In addition, IL-10 production by TLR-mediated PerC B cells was remarkably higher than that by B cells isolated from other parts
of the mice. These results may have been dependent on the unique characteristics of PerC B cells derived from a B-1 lineage. However, when compared with the results of IL-10, lower production levels of TGF-β1 in response to TLR ligands were observed in all Meloxicam of the tested samples (Fig. 2b). In the body systems, TGF-β1 occurs in two physiological forms: latent and active. Although TGF-β1 is important in regulating crucial cellular activities, in most cases an activated TGF-β1 ligand will initiate the TGF-β1 signalling cascade. In our present system, the majority of TGF-β1 as assessed was solely inactive or latent. We also measured the active form of TGF-β1 but the amount was too low to demonstrate any effects of TLR ligands on their secretion (data not shown). Following our experimental results, we investigated the presence of a regulatory B-cell subset producing IL-10 and TGF-β1 in the intestines of BALB/c mice. Furthermore, we conducted additional experiments to elucidate the role of this intestinal regulatory B-cell subset in the pathogenesis of CD using SAMP1/Yit mice. Development of ileitis in the SAMP1/Yit mice was confirmed by histological examinations.
For 3H-thymidine incorporation, 1 μCi 3H-thymidine (Amersham) was
For 3H-thymidine incorporation, 1 μCi 3H-thymidine (Amersham) was added after 24 h and cells proliferated for another 18 h. For transwell assays (0.4 μm pores, Sigma-Aldrich), either 3 × 105 CFSE-labeled splenocytes were in plate wells and 3 × 105 MDSCs in transwell INCB018424 clinical trial inserts; or splenocytes were in plate wells and MDSCs + splenocytes (1:1) in transwell inserts. After 42 h, proliferation of T-cells in the plate wells was measured. Fas-agonistic Jo2 or control mAb (1 μg/mL) (BD Biosciences) were added to cultures after 18 h. After another 24 h, apoptosis was determined using 7-amino-actinomycin and AnnexinV staining (BD Bioscience). IFN-γ and IL-2 were quantified
using sandwich ELISAs (PharMingen), IL-12p70 by the Bio-plex ProTM kit (Bio-Rad) on the Bioplex 200 system (Bio-Rad). NO2− was measured using Greiss reagent as described [43]. Ninety-six-well microtiter plates (Nunc) were coated overnight (4°C) with HA (50
μg/mL) (Sigma-Aldrich), P-selectin-IgG (BD Pharmingen), or control IgG (10 μg/mL). Wells were blocked with 1% dry milk (2 h, 37°C). DiD-labeled CD8+ OT-1 T cells were resuspended in appropriate binding buffer (P-selectin-binding: IMDM + 2% FCS; HA-binding: RPMI1640, 40 mM Hepes, 0.1% BSA, 2 mM MgCl2), added to the plates, subjected to a short spin, and incubated (30 min, 37°C). Nonadherent cells Venetoclax solubility dmso were removed by gentle washing. Bound cells were quantified by a FLUOstar OPTIMA fluorescence plate reader (BMG Labtech). Cytotoxicity of CD8+ T cells was tested using a 4-h 51Cr-release assay. Spontaneous lysis was measured by incubating target cells only with medium, maximal lysis by incubating with 10% saponin. This work was supported
by a doctoral grant from FWO-Vlaanderen to E.S. and K.M., by a doctoral grant from IWT-Vlaanderen to D.L. and Y.M., and by research grants from "Stichting tegen Kanker" and "Vlaamse Liga tegen Kanker" to P.D.B. and J.A.V.G. The authors also thank Ella Omasta, Marie-Thérèse Detobel, Maria Slazak, Nadia Abou, and Eddy Vercauteren for technical and administrative assistance. The authors declare no financial see more or commercial conflict of interest. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Table S1. Overview of the effects of MO- and PMN-MDSCs on various aspects of CD8+ T-cell activation. Table S2. List of commercial antibodies used for flow cytometry Figure S1. MO- and PMN-MDSCs were purified from the spleens of EG7-OVA tumor-bearing WT, IFN-γR-/-, STAT-1-/- and IRF-1-/- mice. Figure S2. MO- and PMN-MDSCs differentially depend on IFN-γ, STAT-1 and IRF-1 to activate their anti-proliferative capacity. Figure S3.
It has already been demonstrated that the degree of bronchial rea
It has already been demonstrated that the degree of bronchial reactivity to histamine or metacholine correlates with asthma severity measured as symptom scores, treatment required
to control symptoms and diurnal variability of lung function parameters [21, 22]. Interestingly, it has been demonstrated that CD14++ CD16+ cells are potent producers of many pro-inflammatory cytokines while stimulated with viral nucleic acids indicating their possible role in regulation of the inflammatory response [9] Surprisingly, however, we have not been able to demonstrate any direct correlation between the number of CD14++ CD16+ cells and any of the conventional Staurosporine molecular weight parameters reflecting intensity of airway inflammation such as FeNO or peripheral blood eosinophilia. Therefore, we cannot provide evidence that CD14++ CD16+ monocytes significantly affect the intensity of allergic inflammation in response to allergen challenge. However, it cannot be excluded that in asthmatic patients, those cells may affect AHR through modulation of inflammatory response to respiratory infections including viral infections. Dysfunction of the airways seen in asthmatic patients depends not only on airway inflammation but also on structural changes in the airways referred to as remodelling. Although airway inflammation leads to development
of bronchial reactivity, in asthmatic patients, successful therapy with inhaled corticosteroids has only selleck mild effect on AHR [23, 24]. Anti-inflammatory effects of corticosteroids in asthma are Sclareol associated with dramatic depletion of inflammatory cells, mainly eosinophils and lymphocytes from the airway tissues [24]. However, some structural changes in the airways are resistant to corticosteroid therapy [24]. It has been recently demonstrated that the degree of bronchial responsiveness to histamine but not to metacholine correlates with airway remodelling [25]. It is therefore tempting to speculate that the disequilibrium between individual PBM subsets may
participate in the development of airway remodelling and AHR. The CD16+ monocytes play a role in tissue remodelling and angiogenesis [8, 10, 26]. Analysis of transcriptomes demonstrated that among all PBM subsets, the CD14++ CD16+ cells are characterized by the greatest expression of genes involved in tissue remodelling and angiogenesis such as TGFB1 or CD105 [10]. Moreover, the Th-2 type cytokines such as IL-4 and IL-13, which are abundantly produced in allergic asthmatics, induce differentiation of monocytes into profibrotic and angiogenic macrophages, which in turn play a crucial role in remodelling of the lungs leading to pathological fibrosis [27]. Further insights into the potential role of individual PBM subsets in asthma are provided by analysis of their kinetics after allergen challenge. Decrease in the number of CD14++ CD16+ PBMs after allergen challenge may reflect different chemotactic potential of those subsets.
Transfection experiments were carried out essentially as describe
Transfection experiments were carried out essentially as described previously (8). Briefly, viral DNA (1.5 μg/culture) was excised from recombinant plasmid and introduced into the cells using Lipofectamine (Invitrogen, Carlsbad, CA, USA). Thereafter, the transfected cells were transferred into 25-cm2 flasks containing culture medium and passaged at a split ratio of 1:3 or 1:4 every 3 or 4 days. Cells were harvested at 30, 43, and 50 days after transfection, and the HA titer was determined as described previously (8, 14). Experiments
were performed using four independent cultures. The transfected cells exhibited no obvious CPE and were able to be passaged serially for 3 weeks of incubation. Thirty days after transfection, obvious CPE buy Opaganib (rounding
of the cells) was observed in a small population of all COS-tat cell clones (data not shown). The cells were subjected to HA assay at 30, 43, and 50 Tamoxifen molecular weight days after transfection. At 30 and 43 days after transfection, HA titers of COS-tat cell clones were significantly greater than those of parental COS-7 cells (Fig. 1a, b). In COS-tat7 cells, HA titer peaked at 43 days and remained unchanged up to 50 days after transfection (Fig. 1a–c). HA activity in COS-tat15 cells increased gradually from 30 to 50 days, with a peak at 50 days after transfection (640 ± 256 HA units) (Fig. 1a–c). HA activity in COS-tat 22 cells increased steeply up to 30 days compared to that in other COS-tat cell clones (Fig. 1a) and was similar to that in parental COS-7 cells at 50 days after transfection (Fig. 1c). These results indicate that stable expression of
HIV-1 Tat leads to increased production of PML-type JCV in COS-tat cells. The data also suggest that the kinetics of PML-type JCV propagation differ among COS-tat cell clones. To confirm HIV-1 Tat-mediated propagation of PML-type JCV, we examined the replication of viral genomic DNA in COS-tat cell clones. Total DNA was isolated from the above-mentioned HA samples using a QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) and subjected to real-time PCR analysis for quantification of JCV genomic DNA, essentially as described previously (8, 14). The detectable range of real-time PCR was more than 100 copies per reaction in this system (8, 14). The amount of viral DNA in COS-tat7, COS-tat15, and COS-tat22 cells was Aldehyde dehydrogenase significantly greater than that in parental COS7 cells at 30 days after transfection (Fig. 2a). In COS-tat7 cells, viral DNA level peaked at 43 days after transfection and declined at a later time point (Fig. 2b, c). The amount of viral DNA in COS-tat15 cells gradually increased from 30 to 50 days after transfection (Fig. 2a–c). In COS-tat22 cells, the amount of viral DNA increased steeply up to 30 days after transfection compared to other COS-tat cell clones. Although COS-tat22 cells exhibited a steep increase in the amount of viral DNA compared to other COS-tat cell clones on day 30, the amount decreased from 43 to 50 days (Fig. 2a–c).
Future investigations might aim at identifying drug level thresho
Future investigations might aim at identifying drug level thresholds that allow for minimum toxicity and optimum efficacy of antifungal prophylaxis. "
"So far fungal foot infection (FFI) has been considered as troubling, however, not dangerous, by the general public as well as doctors. Nevertheless, new immunology information and anatomy dispositions led us to the distinct suspicions. We propose a FFI–induced knee joint osteoarthritis (OA) model. We suppose repeated recurrences of fungal foot disease to be the initiating immunology impulse. The aim of the work is to introduce a new model and to determine antigen epitopes initiating and maintaining
the knee OA using computer simulation. click here Freely accessible immunological databases
and servers were used in this search. Presentable antigen epitopes in Trichophyton rubrum dermatophyte products were identified for molecules of the six most abundant alleles of DRB1 locus of human major histocompatibility complex. Subsequently, similar sequences in human joint peptides (collagens, aggrecan and others) were matched to these antigen epitopes by a comparative program. A number of pairs with very similar fungal selleckchem and joint peptide sequences, supposed to initiate and maintain the knee OA antigen epitopes, were found. A FFI-induced knee joint OA model is shown to the medical community which can initiate further discussion, research and practical verification. "
"Inflammatory Tinea capitis (TC) is a rare form of TC. The aim of this study was to review epidemiological, clinical and mycological profile of inflammatory TC. We present a retrospective study (1999–2010), enrolled all the cases of inflammatory TC observed at a referral hospital in the northern Tunisia. One hundred and twenty-one patients with mafosfamide inflammatory
TC, 83 male patients (68.6%) and 38 female patients (31.4%) were enrolled. The mean age was about 8 years. A majority of TC (71.9%) were in patients lesser than 10 years of age. Positive family history and contact with animals were noted in seven and 35 cases respectively. Direct examination was positive in 110 cases (59 ectothrix, 51 endothrix) and positive cultures were obtained in 105 patients (49 Trichophyton violaceum, 31 Microsporum canis, 13 Trichophyton interdigitale complex, 12 Trichophyton verrucosum). Systemic treatment was carried out in 115 patients with griseofulvin, in one with terbinafine. A complete recovery was noted in 88 cases; and persistent alopecia in 28 cases. The inflammatory TC is rare, but more common in rural families. The disease mostly affected male genders (68.6%) and T. violaceum remains the common pathogen of inflammatory TC in northern Tunisia. "
"Candida albicans is the major aetiological agent of oral candidosis and one of its important virulent factors is the production of extracellular phospholipases, which can be modulated by subtherapeutic concentrations of antifungal agents thus decreasing their pathogenicity.
To investigate whether the orphan gene cluster is responsible for
To investigate whether the orphan gene cluster is responsible for the biosynthesis of these complex polyketides, we analysed its architecture Ruxolitinib order and compared it to the gene cluster encoding enacyloxin biosynthesis in B. ambifaria.[53] Indeed, we found a high
similarity of both clusters (Fig. 1b). The PKS consists of various proteins with similarity to cis-acyltransferase PKSs and a single protein with homology to trans-AT PKSs. Additionally, a number of tailoring enzymes such as oxidases and chlorinases are encoded in the gene cluster. The absolute configuration of the carbons was inferred from the deduced stereospecificity of the ketoreductase domains and is in full accord to the configuration predicted for enacyloxins in B. ambifaria.[53] Enacyloxins possess potent antibiotic properties due to their ability to inhibit protein synthesis by binding to the elongation factor EF-Tu.[54, 55] By agar diffusion assay, we tested the antibacterial activity of the novel derivative 6 as well as of enacyloxin IIIa (5) and found that both compounds display equally potent activity against E. coli and P. aeruginosa. Next, we investigated whether enacyloxins are also produced in the fungal–bacterial coculture. Therefore, we cultured both organisms on an agar plate and analysed product formation by HPLC-MS. Surprisingly, we found high titres of antibiotics (1–2 mg l−1) in
the mixed cultures as well, indicating that enacyloxins may also be produced during the food fermentation process. check details We also noticed a strong growth inhibition of the fungus when grown next
to the bacterium (Fig. 3A). Even more surprisingly, a characteristic phenotype became apparent: Whereas Ketotifen the fungal cells are retarded in growth, the bacteria seem to grow to a high cell density in vicinity to the fungus. In addition, we noticed the appearance of a distinct yellow line on the bacterial–fungal interface, presumably a precipitation of a secreted compound (Fig. 3A). To elucidate the nature of the precipitate, we cut the line from the agar plate and extracted the agar plug with ethyl acetate. LC-MS analyses of the extract revealed that the line is caused by precipitation of bongkrekic acid (Fig. 3A). Bongkrekic acid is known to possess antifungal activity,[18, 56] indicating that the strong growth inhibition of the fungus is due to a massive secretion of bongkrekic acid. Therefore, we analysed the activity of bongkrekic acid against R. microsporus by agar diffusion assay and found that the toxin is indeed active against the fungus (MIC 20 μmol l−1). In this respect, it is also interesting to note that we noticed a huge increase (100%) in bongkrekic acid production when the bacterium is grown in presence of the fungus. This finding implicates a high production rate during the food fermentation process. Next, we investigated the cause of the precipitation of bongkrekic acid.
1) Analysis of 15 normal, uninfected PPD-negative healthy donors
1). Analysis of 15 normal, uninfected PPD-negative healthy donors revealed no detectable cytokine expressing CD4+ T cells after stimulation with the M. tuberculosis proteins, ESAT-6, Ag85B and 16 kDa (Table 1), thus confirming specificity of intracellular cytokine
staining. Following stimulation with Staphylococcal enterotoxin FK506 nmr fragment B (SEB), the proportion of 3+ CD4+ T cells, which produced IFN-γ, IL-2 and TNF-α simultaneously, was very low and did not differ statistically between TB patients and subjects with LTBI (data not shown). Similarly, there was no statistically significant difference in the proportions of 2+ CD4+ T cells (IFN-γ+IL-2+, IFN-γ+TNF-α+ and/or IL-2+TNF-α+) between TB patients and LTBI subjects, but the latter had a significantly click here lower proportion of 1+ TNF-α+ CD4+ T cells (data not shown). There were a number of differences between TB patients and subjects
with LTBI following stimulation with ESAT-6, Ag85B and the 16-kDa antigen (Fig. 2). Most notably, and in contrast with the previously reported results in chronic viral infections, we found a significantly higher proportion of 3+ CD4+ T cells simultaneously secreting IFN-γ, IL-2 and TNF-α in patients with TB, as compared with LTBI subjects, upon stimulation with any of the three tested M. tuberculosis antigens (Fig. 2). Using a threshold of 0.01% to avoid systematic biases incurred by zeroing negative values (frequency
values <0.01% were set to zero), we found that 3+ CD4+ T cells were detectable in very few LTBI subjects (3/18, 3/18 and 2/18 in response to Ag85B, ESAT-6 and 16 kDa, respectively), but were frequently detected in most TB patients (17/20, 18/20 and 17/20, in response to Ag85B, ESAT-6 Astemizole and 16 kDa, respectively; see also Table 1 for comparison). In contrast, LTBI subjects had significantly higher (12- to 15-fold) proportions of 2+ CD4+ T cells that produced IL-2 and IFN-γ (IFN-γ+IL-2+) in response to Ag85B, ESAT-6 and 16 kDa, compared with TB patients (Fig. 2). Moreover, LTBI subjects also had higher proportions of 1+ CD4+ T cells that produced IFN-γ only (IFN-γ+), compared with TB patients, although this difference attained statistical significance only in response to Ag85B. Proportions of any other 2+ or 1+ cytokine secreting CD4+ T-cell subsets did not differ between TB patients and subjects with LTBI after short-term antigen stimulation (Fig. 2). This suggests that the type of response is not determined by the type of antigen, but is rather homogenous against the whole pathogen. It has been previously reported that LTBI individuals with a negative short-term (24 h) IFN-γ release test (IGRA) may turn to a positive response after long-term (6 days) stimulation 21.
435, P = 0 038) and weakly with dialysis vintage (n = 60, r = −0
435, P = 0.038) and weakly with dialysis vintage (n = 60, r = −0.216, P = 0.050). Serum Fet-A RR, on the other hand, selleck screening library were positively correlated with log-transformed serum CRP concentrations (Fig. 3; r = 0.338, P = 0.002) dialysis vintage (n = 60, r = 0.508, P < 0.001), and weakly with calcium carbonate dosage (r = 0.345, P = 0.047). Neither serum total Fet-A concentrations nor Fet-A RR showed significant differences with respect to gender. Inflammation and mineral stress, as commonly seen in patients with CKD, are associated with detectable
levels of CPP in the circulation. CPP formation may prevent further mineral aggregation, crystallization and progressive crystal growth, but may also deplete levels of free Fet-A that may have protective cellular effects. Calcium phosphate nanocrystals are pro-inflammatory to macrophage, stimulating the production of pro-inflammatory cytokines and reactive oxygen species and are thus by themselves damaging.[24] Therefore, CPP formation
may be viewed as a response to mineral stress to prevent systemic mineral deposition. Recent work describes the rapid uptake of CPP by the reticuloendothelial system,[15] thereby removing potentially damaging packets of mineral and preventing their aberrant deposition. ABT-263 purchase These data are certainly congruent with this theory. The fact that these CPP are not normally detectable in the circulation, and that mechanisms of clearance exist, suggests that in pathological states, either the rate of formation is increased or the rate of removal is reduced
or at least exceeds the capacity of the clearance pathway. There is good in vitro evidence that free Fet-A is internalized by mineral-stressed VSMC, wherein it inhibits caspase-induced apoptosis and matrix-vesicle mineralization,[34] both key steps in VC. Hence limitation of free Fet-A by consumption in the formation of CPP may exacerbate the situation. Alternatively Fet-A-containing CPP may be taken up by macrophage or VSMC and may themselves have deleterious cellular effects. In this paper we again show that CPP are detectable in CKD and are present at high levels in patients Phloretin undergoing dialysis as indicated by the high serum Fet-A RR. The slightly higher average Fet-A RR in HD compared with PD patients presumably in part reflects lower systemic inflammation observed in some PD patients, but also their shorter dialysis vintage. If the removal of CPP were merely a function of renal function then one might expect to find the absence of such particles in conditions where renal function is normal. We recently reported a case of Takayasu's arteritis which was associated with gross VC, raised serum Fet-A RR but normal renal function.[31] We have extended this observation in this study by showing that the presence of chronic inflammation per se appears associated with elevated serum Fet-A RR, even in patients with normal renal function, suggesting a role for inflammation in the genesis of these particles.


text:
UncategorizedThe Bold Italic ceases operations
The Bold Italic ceases operations
By Marke B.
Gannett-owned experiment in hyperlocal coverage – with cute illustrations — suddenly shuts down.
APRIL 7, 2015 — A message posted minutes ago to the Bold Italic announced that the conglomerate-owned yet locally operated website will cease operations immediately:
Since our launch, The Bold Italic has strived to faithfully serve San Franciscans near and far, whether born and bred here or having just arrived in the city. We have a great passion for the Bay Area and all that it has to offer — and have had a lot of fun sharing our enthusiasm with you. Together we have built a strong community of followers, contributors, and partners. However, we have made the difficult decision to cease operations. It's been a great run and we supremely thank everyone who has supported us along the way.
Via email, Bold Italic editor Jennifer Maerz confirmed the closure. She wrote: "Today we made the very difficult decision to cease operations at The Bold Italic…. We're proud of the stories we've told about this amazing place where we live and work. While we will stop publishing new stories today, the site will stay live for several months to allow time for [freelancers] to capture and archive content for portfolios or clips."
When Bold Italic launched, it was positioned as a hyperlocal media experiment, attempting to bring local web coverage with a certain personality and broad appeal into the tough SF market.
Gannett, the multinational media company headquartered in Virginia that owns the Bold Italic (the concept was developed with Palo Alto company Ideo in 2009) had been struggling to balance its desire for online penetration with its legacy of newspapers. In August of last year, the company made the decision to spin off almost all of its newspapers, including USA Today. The company made gestures toward rebranding itself as a "broadcast and digital company."
At that same time, I received a curious email from Gannett. (I was publisher of the Guardian at the time.) Originating from the head of the sales department, it informed me that Gannett was offering Bold Italic for sale, to the tune of 5+ million. Welp, I didn't bite. Also at the same time, Bold Italic expanded its coverage to LA. The mixed messages were confusing to say the least.
And then there was the online media outlets' fancy digs in Hayes Valley, which must have cost a dime or two. (The Bold Italic also threw events — usually a good moneymaker – like its "micro hoods" street parties.)
But now, the whimsical maps, sometimes polemical/sometimes eye-rolling articles, broad newcomer appeal, and heroic productivity of editor Maerz (an excellent editor formerly of SF Weekly) and her stable of writers and illustrators at the Bold Italic exist in suspended animation, at least for a few months more.
UPDATE Maerz has posted her own farewell message.
UPDATE As for the precise reason why the Bold Italic shut down, that remains a mystery. Gannett representatives have declined to comment. This SF Gate article fills in some of the background of Gannett's financial struggles.
Marke B.
Marke Bieschke is the publisher and arts and culture editor of 48 Hills. He co-owns the Stud bar in SoMa. Reach him at marke (at) 48hills.org, follow @supermarke on Twitter.
'No Straight Lines' told the hidden history of queer comics—now it's a movie
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Tamara Palmer -
Sweeping in the rain
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The story behind the garden-hose assault outside a North Beach gallery
Sup. Peskin's office has been working for years to get help for this homeless person—and city agencies have completely failed them.


text:
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Join the staff!
Keeping in time with the Mills Community since 1917
Campus thefts and vandalism
By Halie Johnson on November 10, 2005
Three suspicious individuals pulling on door handles at the education complex were reported to Public Safety around midnight on Wednesday, Nov. 2 by a residential assistant.
Also, at 5:30 a.m. on Thursday, Campus Facilities reported vandalism and thefts at the corporation yard, the campus facilities grounds behind Founders Commons on the Seminary side of campus.
Niviece Robinson, operations superintendent and interim director of public safety, said the Oakland Police Department reported the incident at the corporate yard at 10:30 a.m.
Seven Mills facilities trucks were vandalized – windows broken, dents made – and tools were stolen from several of the vehicles. Paul Richards, the director of campus facilities, was away for a conference, so there is not yet an assessment of collateral damage.
"The primary areas of concern have been the Education Complex and the CPM [chemistry, physics and mathematics] building, securing the Oval," Robinson said.
She said one of her goals is to increase security at the corporate yard by installing video cameras and upgrading the existing fence to an iron rod fence, making it easier to see when there has been a breach of security.
According to Robinson, the suspects seen at the Education Complex were not identified. A janitor who was working in the building first saw the three and called for backup. A resident assistant assisted her in translating to Public Safety what she saw. The second guard on duty left the front gate to assist the first at the education complex, so for a short time the gate was unguarded.
An Oakland Police detective has been assigned to the case and met with Robinson on Tuesday, Nov. 8 to discuss the investigation.
Robinson said that in the coming months she will be working with individual departments to upgrade security by installing cameras.
Halie Johnson
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text:
China Regulator Says Tesla Recalls More Than 80,000 Cars
The logo of Tesla in Taipei, Taiwan, on Aug. 11, 2017. (Tyrone Siu/Reuters)
November 25, 2022Updated: November 26, 2022
BEIJING—Tesla Inc. is recalling more than 80,000 China-made and imported cars produced from as early as 2013, for software and seat belt issues, a statement by the Chinese market regulator revealed on Friday.
The U.S.-based electric car maker has recalled 67,698 Model S and Model X cars imported to China between Sept. 25, 2013 and Nov. 21, 2020, due to software problems affecting the battery management system in the vehicles.
Tesla said it will upgrade the software of recalled vehicles.
Tesla also recalled 2,736 imported Model 3 cars manufactured between January and November 2019, and 10,127 China-made ones of the same model produced between Oct. 14, 2019 and Sept. 26, 2022. This was due to potentially faulty seat belt installation which Tesla will check and reinstall, it said.
In April, Tesla recalled a total of 127,785 units of Model 3 cars in China, citing potential faults in semiconductor components that might lead to collisions.


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Jargon is a great way to talk about complex issues in a brief way – but only if you understand it. Isn't it frustrating when you're talking to someone and they use some jargon you've not heard of before and you then feel a little shy about asking for an explanation – well that's just how other feel when you do it.
Jargon can be very powerful to communicate when it's between people who understand the concept – at any other time it's just "tecno-babble" that clouds the issue and really shows that you're not really trying to communicate with someone but impress them with your technical word skills.
A good response to someone using techno-babble on is to simply say the jargon word or phrase back in a questioning tone – this will always get a explanation and sends a simple message that the person who's talking to you is not connecting properly and that they need to change their way of talking to one that you both can relate to.
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text:
AMR: EU Commission awards €45,000
EU Health Commissioner Vytenis Andriukaitis, © Audiovisual Services of the EC
Vytenis Andriukaitis, Commissioner for Health and Food Safety, has awarded the EU Health Award for NGOs fighting Antimicrobial Resistance to BEUC for lobbying to reduce the threat of antimicrobial resistance (AMR).
Annually, AMR causes 25,000 deaths and over €1.5bn in healthcare costs and productivity losses in the EU alone. The same number of deaths is reported for the US. However, due to lack of figures, particularly in countries of the global South, the global impact can be only estimated. AMR has become a political priority globally. The European Commission, and the G20 have been supporting the WHO's AMR action plan, and the Commission announced it will update its measures to combat antimicrobial from 1999 by summer 2017. The new Action Plan against AMR will be based on the One-Health approach, which recognises that the health of people, of animals and of the environment are inextricably linked.
The prize aims at rewarding outstanding initiatives by NGOs that significantly reduced the threat of antimicrobial resistance (AMR) to human health. Andriukaitis awarded the €20,000 first prize to BEUC, the European Consumer Organisation, for their campaign "From Farm to You", which has raised awareness about the causes of AMR, such as overuse of antimicrobials in livestock and misuse and overuse of antibiotics in human medicine.
At the award ceremony hosted by the University of Leuven in Belgium, the Commissioner said: "Averting this looming threat before it turns into a public health nightmare is my most pressing priority as Health Commissioner, and as a former doctor. I count on the continued help and commitment of organisations like BEUC."
The second prize of €15,000 went to both the Alliance to save our Antibiotics, Compassion in World Farming and the Soil Association for their campaign to end the routine prophylactic mass-medication of farm animals.
Andriukaitis awarded the third prize of €10,000 to the World Alliance against Antibiotic Resistance for their campaign "Acting to Preserve Antibiotics" aimed at limiting the surge and spread of multi-drug resistant pathogens.
On 24th February, a number of experts from all over Europe will discuss current challenges in the antimicrobials market in Europe during the Berlin Conference on Novel Antimicrobials.
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EC signs prepurchase agreement with Valneva
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Tuition for the entire program is only $325.
The 8-week long Red Zone Challenge has been an exciting part of my offerings over the last three years. We give you all the tools to become a great short game player.
The program starts out with a 90-minute individual session. We first run you through an assessment that covers all of the critical areas involved in the short game.
At the end of the assessment you will have a Short Game Handicap. In the same session we will get right to work on the one area that you personally need the most. After that we get into the group classes. There will be multiple classes scheduled for each of the short game areas. I schedule multiple classes for two reasons; so everyone can fit at least one of each topic into their calendar and so that students can attend a number of classes on the topics that they need the most help.
At the end of the session you will have a chance to retake the assessment to see just how much you improved. Along with the private session and the classes each participant receives an additional private one-hour lesson.
The next session of the RZC will begin this Spring. Take the initial assessment between now and May 9th. Class size is limited so reserve your spot today!


text:
Q: How to fill area with custom color under line in nvd3 linechart ? I'm working with nvd3 and I'm not much hands-on with its styling (css properties) to customize it. I have a line chart with two data lines on it.
The code for drawing the lines is following:
nv.addGraph(function() {
        chart = nv.models.lineChart();

        chart.margin({
            left : 100,
            bottom : 100
        }).useInteractiveGuideline(true).showLegend(true).duration(250);
        chart.xAxis.axisLabel("Date").tickFormat(function(d) {
            var date = new Date(d);
            return d3.time.format("%b-%e")(date);
        });
        chart.yAxis.axisLabel('').tickFormat(d3.format(',.2f'));

        chart.showXAxis(true);

        d3.select('#startupRiskLineChart').datum(
                prepareDataObj(val1_y, val1_x, val2_y, val1_x))
                .transition().call(chart);
        ;
        nv.utils.windowResize(chart.update);
        chart.dispatch.on('stateChange', function(e) {
            nv.log('New State:', JSON.stringify(e));
        });
        return chart;
    });

Is there any way to fill the area under data line with a lighter color?
Any help would be highy apperiated.

A: To get a line chart with a filled area in a specific color:

*

*Add area: true to the data series, as in this example.

*Add this CSS: .nv-area { fill: red }
The opacity of the area is set to 0.5, so filling it with red will actually make it pink:

A: chart.color(["red","yellow","blue"]);

'#00FFFF','#0FF','cyan' all are valid ways to set colors

Another way is set it in data itself
    [{
      values:[{x:1,y:1},{x:2,y:4},{x:3,y:9},{x:4,y:16}],
      key: 'not a Sine Wave',
      color: '#ff7f0e'  //color - optional: choose your own line color.
    }]

duplicate question i guess

A: Add
classed: "my-custom-approx-line"

to config of specific line and add styling:
&.nv-area {
  fill-opacity: 0.2;
}

A: As of commit #becd772
Use .nv-group{ fill-opacity: 1.0 !important; } to fill the area with the same color as the line

redpajama_set_name: RedPajamaStackExchange


text:
'Don't Speak' Gwen Stefani Tells Gavin Rossdale. No Really, Because She's Divorcing Him
By William Twiddlesmith August 4, 2015
Another Hollywood marriage bites the dust. First, Ben Affleck and Jennifer Garner. Now, Gwen Stefani and Bush frontman Gavin Rossdale. It only took 13 years of marriage for Stefani to realize Bush's music sucks balls. They haven't made any good songs ever. They may have had popular songs, but never any good songs.
Stefani cited the old irreconcilable differences excuse in her divorce. So did Rossdale.
Gwen, who's repped by attorney Scott Weston, is asking for joint custody of the couple's 3 kids — 9-year-old Kingston, 6-year-old Zuma and 1-year-old Apollo.
Gavin, who's repped by disso-queen Laura Wasser, filed his response at the same time, and he's also asking for joint custody. It's clear the petition and response were orchestrated to be filed simultaneously.
This next part is a kicker:
Sources tell TMZ there is no prenup, so the 50/50 California community property laws kick in. 45-year-old Gwen is worth a reported $80 million, and 49-year-old Gavin's worth an estimated $35 million. Given the length of the marriage, that will probably all go into one pot before it's divided.
Stefani having $80m to her name, not a surprise with all the No Doubt songs and her gig on The Voice. But Rossdale is worth $35m??? No Bush songs could make him that much money. He must be a drug kingpin, obviously the only plausible explanation. Selling Ecstasy to his fans. That's a joke, please don't sue.
Their joint statement ends by saying the two "are committed to jointly raising our three sons in a happy and healthy environment." This can only mean a house free of horrible alternative rock songs.
#divorces
#gavin rossdale
#gwen stefani
Partner highlights
Gavin Rossdale's Hot Young Model Girlfriend Sophia Thomalla Has a New Hot Young Athlete Boyfriend
Michael Moore Says Donald Trump Ran for President Because Gwen Stefani Made More Money Than He Did
Gavin Rossdale Getting Catfished by Elin Nordegren


text:
Warrants Detail Sex, Drugs in Death of Utah Teen
A Utah couple implicated in the death of their teenage babysitter was apparently involved in a drug-fueled, salacious relationship with the 16-year-old girl, and after something went awry, devised a plot to get rid of her body, according to court records released Tuesday.
October 25, 2011: Eric Millerberg stands next to his public defender Randall Marshall during his sentencing for a drug violation at the 2nd District Court in Ogden, Utah.(AP)
SALT LAKE CITY – A Utah couple implicated in the death of their teenage babysitter was apparently involved in a drug-fueled, salacious relationship with the 16-year-old girl, and after something went awry, devised a plot to get rid of her body, according to court records released Tuesday.
Alexis Rasmussen vanished Sept. 10 after babysitting for Eric and Dea Millerberg in North Ogden, about 45 miles north of Salt Lake City, police said. Her body was found Oct. 18 off a nearby interstate after authorities received a tip from a confidential informant.
Last week, Weber County prosecutors named the couple "persons of interest" in Rasmussen's death, but have declined to elaborate. Dea Millerberg's attorney Michael Bouwhuis has said he's been told his client would likely be charged in the case with counts ranging from desecration of a body to capital murder. Authorities have declined to comment.
In court records released Tuesday, and obtained by KTVX-TV and the Standard-Examiner of Ogden, witnesses say Rasmussen was paid for her babysitting services with drugs on the night she vanished. One witness told authorities that a 16-year-old girl had sought sex from the couple. It was not immediately clear from the news accounts whether the witness was referring to Rasmussen.
The search warrants were filed Tuesday morning in 2nd District Court in Ogden, but were later ordered sealed by a judge. In the Oct. 9 search of the Millerberg's home, police said they found traces of human blood in an area where carpet had been removed, according to the records.
More On This...
Couple named 'persons of interest' in teen's death
"Dea knows everything that happened. The girl is buried somewhere in the state, she's not gone, but not alive," a witness told authorities shortly before Rasmussen's body was discovered, the records indicate.
The warrants also say witnesses told investigators they believed the Millerbergs were involved in Rasmussen's death, and that both participated in a plot to dispose of her body.
Neither of the Millerbergs' attorneys returned calls from The Associated Press seeking comment. Rasmussen's father also didn't return a telephone call Tuesday evening. Rasmussen's step-grandfather, Mike Miera, said the family had no immediate comment.
Dea Millerberg is jailed on unrelated prescription drug fraud charges pending a Nov. 16 hearing. Her husband has since been sent to prison for violating probation in a 2010 fraud case after authorities say he was found with heroin and Oxycontin and had failed to report to his probation officer.
Weber County Attorney Dee Smith has only said that Rasmussen died in a house on or around Sept. 11 and that her body was moved to a grave near Interstate 84, about 35 miles from North Ogden.
Rasmussen's mother, Dawn Miera, has said her daughter was babysitting the Millerberg's two young children on the night she disappeared. At about 10:30 p.m. that night, the teen was seen with Dea Millerberg on surveillance video at a nearby all-night pharmacy, Dawn Miera said.
She said she also received text messages from her daughter about an hour later indicating she was back at the couple's home, and still babysitting because the Millerbergs had left to resume their date. It wasn't clear why they had returned to go to the pharmacy.
Miera said she told her daughter to stay the night because it was so late. The girl vanished soon after.
The Millerbergs told police that Rasmussen left their home before midnight to meet a friend, but police said that person never saw her.
Senate votes down controversial House budget
New Jersey School Bus Driver Accused of DUI
Reports of sea lion shootings on rise in Calif.
Calif AG: Man with beard can't become prison guard
Stocks soar after Fed pledges low rates into '13
GOP open to discussing concerns on TN Shariah bill


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A step back toward the anti-drilling Obama era may cause the energy industry to cut jobs, lower investment in energy and reduce our GDP.
Hiking the tax on carried interest capital gains would discourage entrepreneurs who invest their time, energy and expertise in American businesses.
Former White House special counsel Ken Starr on the political fallout from the Mueller report.
Woodford Reserve Master Distiller Chris Morris and 2019 Derby Bottle artist Keith Anderson on being the official bourbon of the Kentucky Derby.
Hedge fund manager Jonathan Hoenig and Altium Wealth Portfolio Manager Michael Lee on the Pinterest and Zoom IPOs, the lawsuit against Lyft by an investor and the outlook for the U.S. economy.
Louisville, Kentucky, Syracuse, New York, Chicago, San Diego and Miami will each receive $3 million dollars.
The billionaire investor wants to make public education and economic opportunity better in the state.
James Patterson is America's highest paid author.
Americans don't realize that they need money for their own health care during retirement, said Chris Hogan.
Today's retirees are living it up. Their secret? They started retirement planning early on.
Federated Investors Senior Equity Strategist Linda Duessel on the upcoming March jobs report, the state of the U.S. economy and the outlook for stocks.


text:
Step by step: Making your own movie trailer
Compile material for your trailer
Select a trailer template and add content
Edit your trailer in more detail (optional)
Create your film trailer and share it with the world
Please select ... Compile material for your trailer Select a trailer template and add content Edit your trailer in more detail (optional) Create your film trailer and share it with the world
Our product: Movie Studio Platinum
Create your own film trailer with Movie Studio Platinum
– here's how it's done.
Want to make a movie trailer? Keep reading to learn how.
Start Movie Studio Platinum and under the "Templates> tab in the Media Pool, open the film and cutting templates.
Pick a template for your trailer. Load the template using the Import icon or simply drag and drop the template into the project window.
Drag video scenes or still images from the Media Pool onto a placeholder and add a title.
Export the trailer as an MP4 or upload it directly to your Vimeo or YouTube account.
Movie Studio 2023 Platinum
Create film trailers with Movie Studio Platinum, the video editing program for ambitious movie makers:
Massive selection of trailer templates
Use as many video scenes and photos as you want in your movie trailers
Easy-to-use Trailer Wizard
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Trailers: From exciting movie teasers to stand-alone video clips
Film trailers are smaller video clips that are designed to create interest in a full-length film. They appear on social networks and websites or are shared via email to create excitement about a movie and attract viewers. But trailers aren't just used to promote a feature film or TV series – they can be used for all kinds or events and occasions too. For instance, a trailer can be created to provide video commentary for current events, or simply as an end in itself.
Trailers are short – usually not longer than 2-3 minutes. They usually contain a captivating intro, fast cuts, music score, a few titles that fade in and occasionally a voice-over. A film trailer doesn't have to be complete as the focus is not on providing information – it's solely to spark interest and make people want to see the film itself.
At the start of any movie trailer production, you have to choose which material you want to include in the trailer. So, when you're producing a movie trailer, it's best to look for scenes from the film that will capture people's interest. You might already have favorite passages in mind that you think would be perfect for making a trailer. For an interview, for example, look for key statements or stand-out exchanges in the conversation. But remember, no one likes a trailer that gives too much away – your trailer should be free of spoilers. Even if your trailer has been made for purposes other than promoting a film, you'll still need video material or photos for your trailer.
Start by creating a kind of material vault where you'll store all your movie clips, still shots or audio passages. Trailer creation is simple when you use Movie Studio video editing software. Movie Studio delivers a whole range of trailer templates that you can use to easily create professional movie trailers using your content. You can use the Trailer Wizard to easily swap out the existing placeholders with your own footage. As a result, you'll be able to make a great trailer with the right design and tempo in no time at all.
If you want to spend a bit more time on your trailer, you can edit it further. For instance, you can change the music included in the trailer template to another track of your choice or record a dedicated commentary. In the editing mode in Movie Studio, you've got access to all the functions provided by this comprehensive video editing software.
Want to make your own trailer from scratch without using a trailer template? No problem. You can combine any number of videos and photos, cut them to the perfect length and have them fade in and out of each other exactly the way you want. Enjoy total creative freedom when it comes to using effects and image animation, too: Have movie scenes fly in from off-screen, shrink them, zoom in on them, move them straight from one end of the screen to the other. If you can imagine it, you can do it.
Keep reading to learn more about trailer creation with Movie Studio through our practical step-by-step guide.
Create a film trailer in Movie Studio
Step 1: Compile material for your trailer
Start by compiling all the video scenes, still shots or photos that you want to use in your movie trailer. To cut out scenes from a longer film, proceed as follows:
Load the video file with the film to a Movie Studio project. Drag and drop the file from the Media Pool into the project window.
Right-click the Mouse Mode button in the toolbar and select Split and trim.
Drag the pointer slowly over the video object. You can follow the video scenes that the pointer moves over in the preview monitor.
One you have found a scene that you would like to use in your trailer, in the video object in the track, click once before the scene and once after it to cut it. You don't need to cut in the exact position, since the cuts you make can be trimmed later using the Trailer Wizard. At this point we just want to create rough cuts of the most important movie scenes. And also, don't worry about changes to your film file, as it's only cut "virtually". The video data on your hard drive will remain unchanged.
Your Movie Studio now contains a number of cut video objects, which you can now export one-by-one as video files. Set a range for each object by placing the range above it. To do this, left-click above the first track to set the beginning of a range and right-click further right after the object to set the end of the range. The range will appear as a blue bar that runs between these two positions. Again, a rough selection is fine here – the range used in the trailer can be adjusted at a later point.
Tip: If you want to set an exact range over a cut object, move the playback marker to the object start using the shortcut Alt + Q, press the I key (for in-point),to set the start of a range, then set the playback marker at the object end using Alt + W and press the O key (for out-point).
Go to "File" > "Export movie" > "Video as MAGIX Video" and in the export dialog, activate the option "Only export selected range". The MXV format is recommended for file transfer within the program. Export all ranges one-by-one as MXV files in this way.
If you want to include still shots in your trailer as well as scenes from your movie, position the playback marker at the location where you want to insert the still shot and then select "File" > "Export movie" > "Single frame as BMP file...".
Step 2: Select a trailer template and add content
In the "Templates" tab, open the film and cutting templates. Film templates contain complete trail templates with transition effects, intros, outros and background music. Editing templates contain cutting patterns and background music. In general, all elements can be modified, deleted, swapped out, or you can add content to an element. This way, the editing templates can be used as a basis when you want to create your own movie trailer.
Hover over a template in the Media Pool. In the pop-up window that appears, click on the middle arrow symbol to play a preview of the template. Using the previews, try to find a template that most closely resembles the idea you have in mind.
To use a template, click on the left arrow symbol in the pop-up window that appears. Or simply drag and drop the template into the project window. This loads it as an additional film into the current project and opens the Template Wizard.
Drag & drop the scenes and photos from their storage location to this placeholder. Virtually all video formats (e.g. MXV, MP4, M2S) and image formats (JPG, BMP) can be used.
If you want to make things even easier, you can select multiple scenes at once while holding down the Ctrl key and dragging then into the editing area below. This automatically fills the placeholder.
Spare placeholders cannot be deleted, as this would muddle up the audio track. It's best to fill all placeholders in a template with video material. If you still want to delete some placeholders, switch to the normal editing view, which includes all the functions you need (see Step 3 below).
After inserting content you can modify the scene section. You can use the info window below right for this. Move the movie strip in the info window until the section "sits" in position. This way, you can use a longer scene for several placeholders be assigning a different section of the scene to each placeholder.
Title placeholders display the label "text". You can enter your own text for the scene below on the right.
Once you're happy with your trailer creation, you can skip the next step and export the trailer as a video (see Step 4 below).
Step 3: Edit your trailer in more detail (optional)
If the trailer's not quite the way you imagined, you can switch to detailed editing mode.
Click the tick above right in the info window to close the Wizard and to open your trailer in the standard Movie Studio editing mode.
Scroll the view down using the scroll bar on the right so you can see the elements on the lower track. Sometimes as many as 50 tracks contain objects. You can enlarge the track range by positioning the mouse at the upper border and dragging the range upwards.
To delete empty placeholders or the music, select the objects you want to remove and then delete them. The audio object with the music is easily recognized. This is the longest object, stretching from the beginning of the trailer to its end. You may need to deactivate the lock symbol, located on the left in the track header, beforehand – if active, this protects all objects on the track from accidental changes.
To load different music, open a Media Pool folder containing audio files and drag the audio file you want to an empty track. To trim the audio, use the mouse to move the end or beginning of the audio object on the track.
To adjust cuts between scenes, move the mouse to the interface between two objects until the mouse pointer turns into a double arrow. You can now fix the borders between the objects.
If you want to record commentary over the scene, click on the red recording button underneath the preview monitor to activate audio recording. To record commentary, you'll need a microphone that is set up and ready for use.
Additional media can be easily dragged from the Import tab of the Media Pool to the position you want in the trailer. The "Effects" tab contains a wide range of effects, not just for image and sound optimization, but for creating image collages or animating photos.
Step 4: Create your film trailer and share it with the world
Once you play your trailer in Movie Studio and are happy with how everything looks, you can export the trailer as a video file or load it directly to your YouTube account from the project.
Under "File" > "Export movie", you'll see the full list of files formats supported by Movie Studio. If you're not sure which format to use, "Video as MPEG-4" is usually a good choice.
Above right, you will see the dialog "Finish movie" top right. You can now transfer the trailer to a connected smartphone, for instance, or upload it directly to your YouTube account.
Movie Studio Platinum: One of a kind – just like your movies.
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How to make your own trailer


Great Plains Purchase Order Processing (POP) module makes up one-third of the core Great Plains Inventory and Order Processing group (the other two being Inventory Management and Sales Order Processing). POP seamlessly integrates with other Great Plains modules (GL, PM, FA, SOP, etc.) allowing for a tightly-knit information system. Gone are the days when invoices from purchases have to be asked from other departments. Great Plains POP module empowers its users by allowing access to mission-critical information including audit control and purchase activity analysis (with proper security permission, of course).
Great Plains prides itself of being able to empower its users in streamlining business processes by being in control. It also improves accuracy and increases employee productivity by automating routine purchasing tasks. POP lets you establish long term contracts using blanket purchase orders to record and track extended purchases, giving you greater negotiating power with your vendors and simplifying contract purchase processes. The latest versions include capabilities to accurately record and track landed costs as well as auto-generation of purchase orders.
? Multiple Ship-To Addresses ? Enter a ship-to address for each line item on a purchase order, reducing documentation for items shipped to multiple addresses.
? Mapping ? Map purchase order line numbers to line items, automatically, to expedite purchase orders.
? Alerts? Establish alerts that prevent employees from issuing invalid purchase orders.
? Flexible Control of Processes ? Control purchasing processes with cost variance tracking, easy transfer of information, receiving/invoicing against multiple purchase orders, comprehensive receipt information, posting, and 1099 reporting.
? Line-Item Level Visibility ? Manage purchase orders at the line-item level with automated roll-up of similar items, free on-board (FOB) designation, individual line item release to vendor dates, and multi-site delivery.
? Purchase Order Tracking - Get instant answers with complete tracking of open or historical purchase order and receiving documents by order number, item number, or date.
? Audit Control? Maintain audit control with cancelled item tracking, line-item ordering and status information, document revision tracking, and customized landed cost definition.
? Multi-Currency Transactions ? Enter, track, and report multicurrency transactions in the originating or functional currency, translated using exchange rate tables, with special functionality for European Union customers.
? Drop Shipment ? Drop-ship orders directly from supplier to customer and bypass the typical inventory update process.
? Reports ? Analyze purchase activity with Purchase Order Status, Invoice Cost Variance, and posting reports. Integrate with Microsoft Great Plains modules such as Crystal Reports for further report flexibility and power.
? Requisition Management for Microsoft Business Portal ? Eliminate paper-based requisitions by creating requisitions via a Web browser, with automatic approval routing and e-mail notification. View purchase requisitions in process as they route from employees to supervisors and purchasing, with customized tracking and validation of commitments against budgets.
? PO Generator ? Automate purchasing through order point or min/max settings. PO Generator determines quantities needed based on the stock situation in relation to the stock goals and generates a suggested purchase order that can be reviewed and edited prior to release. Also, automatically create purchase orders in response to quantity shortages on customer orders and manage these documents from within POP.