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Mucus-Activatable Shiga Toxin Genotype stx2d in Escherichia coli O157:H7 - Volume 23, Number 8—August 2017 - Emerging Infectious Disease journal - CDC
Volume 23, Number 8—August 2017
Research Letter
Mucus-Activatable Shiga Toxin Genotype stx2d in Escherichia coli O157:H7
Technical Appendices
Technical Appendix
PDF[1.64 MB - 3 pgs]
RIS[TXT - 2 KB]
Sergio Sánchez1 , María Teresa Llorente, Laura Herrera-León, Raquel Ramiro, Sandra Nebreda, María Antonia Remacha, and Silvia Herrera-León1
Author affiliations: Institute of Health Carlos III, Majadahonda, Spain (S. Sánchez, M.T. Llorente, L. Herrera-León, R. Ramiro, S. Nebreda, S. Herrera-León); León University Hospital, León, Spain (M.A. Remacha)
We identified the mucus-activatable Shiga toxin genotype stx2d in the most common hemolytic uremic syndrome–associated Escherichia coli serotype, O157:H7. stx2d was detected in a strain isolated from a 2-year-old boy with bloody diarrhea in Spain, and whole-genome sequencing was used to confirm and fully characterize the strain.
The foodborne zoonotic pathogen Shiga toxin (Stx)–producing Escherichia coli (STEC) is responsible for human diseases ranging from uncomplicated diarrhea to the life-threatening hemolytic-uremic syndrome (HUS) (1). Stx production is the most determining virulence factor implicated in HUS, and the intimin, encoded by eae, is the most common adherence factor in HUS-associated STEC (1). Stx2d is a Stx2 variant in which cytotoxicity is increased (from 35- to 350-fold) by the action of elastase in intestinal mucus (2). This mucus-enhanced toxicity is termed "activation," and activatable Stx2d proteins are designated Stx2dact. Stx2dact production and Stx2dact genotype (stx2d) have been associated primarily with eae-negative STEC and considered a predictor for severe clinical outcome in such infections (3).
All STEC strains received or isolated in the Reference and Research Laboratory of Food and Waterborne Bacterial Infections (Majadahonda, Spain) are routinely tested for stx1 and stx2 subtypes by a PCR subtyping method (4). For serotyping, O antigen is identified with both commercial antiserum and PCR (5), and H antigen is identified by PCR amplification of the fliC gene (6) and further sequencing of the PCR product. During 2012–2016, stx2d was identified in 7 (3%) of 236 STEC strains isolated from patients with HUS and/or diarrhea in Spain (193 eae-positive and 43 eae-negative strains). Six were eae-negative non-O157 STEC belonging to serotypes O73:H18 (2 strains), O91:H21, O148:H8, O181:H49, and ONT:H21. Strikingly, the other stx2d-positive strain identified (CNM-2140/12) belonged to serotype O157:H7 and contained stx2d in combination with stx2c, apart from eae. The strain had been isolated from a 2-year-old boy with bloody diarrhea in July 2012, and it fermented sorbitol after overnight incubation on sorbitol MacConkey agar (Becton Dickinson, Sparks, MD, USA). We confirmed Stx2 production using the enzyme immunoassay kit SHIGA TOXIN QUIK CHEK (TechLab, Blacksburg, VA, USA). The activatable property of the toxin was confirmed by partial sequencing of the stx2 gene (4), analysis of the resulting nucleotide sequence by comparison with the published stx2 reference sequences, and comparison of the resulting amino acid sequences. The nucleotide sequence of stx2d (GenBank accession no. MF094370) was 100% identical to that of strain 06–5231 (O55:H7, GenBank accession no. EF584538). The sequence was translated to amino acids (Technical Appendix[PDF - 219 KB - 1 page] Figure), and the activatable property of Stx2d was confirmed with the combined presence of the "activatable tail," the last 10 aa in the C-terminal end of the A subunit (KSQSLYTTGE), and the END motif at position 14–16 in the N-terminal end of the B subunit (4). Stx-associated bacteriophage insertion sites genotyping was performed (7). According to the Stx-associated bacteriophage insertion genotype nomenclature proposed by Shringi et al. (8), the strain belonged to the SY2c genotype; both sbcB and yehV loci were occupied, the former with a Stx2c-associated bacteriophage, plus the additional insertion of the Stx2d-associated bacteriophage within the yecE locus, as recently reported for stx2d-positive STEC O26:H11 (7).
To confirm the genotypic traits mentioned earlier, we sequenced the strain using an Illumina NextSeq 500 next-generation sequencer system (Illumina, San Diego, CA, USA). We extracted DNA using a QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) and generated a genomic DNA paired-end library using a Nextera XT DNA Sample Preparation Kit (Illumina). A total of 1,371,617,258 bp were obtained, providing ≈250-fold coverage and 9,083,558 reads. The sequencing reads have been deposited in the SRA-NCBI public sequence repository (accession no. SRP107062). From the raw whole-genome sequence data, we confirmed serotype O157:H7 using SerotypeFinder; investigated the virulence gene profile, including stx subtypes, using VirulenceFinder (Table); and identified acquired antimicrobial resistance genes using ResFinder (all publicly available on the Center for Genomic Epidemiology server, https://cge.cbs.dtu.dk//services/all.php). In addition, we detected mutations in the quinolone resistance–determining regions of the gyrA and parCgenes in silico on the whole-genome sequence, and no acquired antimicrobial resistance gene or mutation conferring quinolone resistance were identified.
Despite its usual association with eae-negative STEC, we identified the mucus-activatable Stx genotype also in STEC O157:H7, the most common HUS-associated eae-positive STEC serotype. This rare virulence gene combination (stx2d/eae) was recently described in STEC O26:H11 isolated from several HUS patients (9) and in STEC O80:H2 causing HUS and bacteremia in France (10). Although its clinical implications remain unknown—because the strain in our current study was isolated from a patient with bloody diarrhea but not HUS, whereas those isolated in France originated from patients with HUS—these examples show the potential for acquiring and transferring important STEC virulence factors, which can lead to unusual and potentially more virulent strains. Further studies are needed to better determine whether stx2d-positive O157:H7 and other eae-positive non-O157 STEC strains carrying this stx subtype are statistically significant in humans and to determine their clinical implications.
Dr. Sánchez is a researcher at the National Center for Microbiology, Institute of Health Carlos III. His research interests include virulence mechanisms, molecular epidemiology, and antimicrobial drug resistance of diarrheagenic E. coli, especially STEC.
This work was supported by grants MPY-1042/14 and PI14CIII/00051 from "Fondo de Investigaciones Sanitarias" from the Spanish Ministry of Economy and Competitiveness and S2013/ABI-2747 from the Madrid Regional Government. S.S. performed this work while under a research contract from the Miguel Servet program from "Fondo de Investigaciones Sanitarias" from the Spanish Ministry of Economy and Competitiveness (CP13/00237).
Johnson KE, Thorpe CM, Sears CL. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin Infect Dis. 2006;43:1587–95. DOIPubMed
Melton-Celsa AR, Darnell SC, O'Brien AD. Activation of Shiga-like toxins by mouse and human intestinal mucus correlates with virulence of enterohemorrhagic Escherichia coli O91:H21 isolates in orally infected, streptomycin-treated mice. Infect Immun. 1996;64:1569–76.PubMed
Bielaszewska M, Friedrich AW, Aldick T, Schürk-Bulgrin R, Karch H. Shiga toxin activatable by intestinal mucus in Escherichia coli isolated from humans: predictor for a severe clinical outcome. Clin Infect Dis. 2006;43:1160–7. DOIPubMed
Scheutz F, Teel LD, Beutin L, Piérard D, Buvens G, Karch H, et al. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J Clin Microbiol. 2012;50:2951–63. DOIPubMed
Sánchez S, Llorente MT, Echeita MA, Herrera-León S. Development of three multiplex PCR assays targeting the 21 most clinically relevant serogroups associated with Shiga toxin-producing E. coli infection in humans. PLoS One. 2015;10:e0117660. DOIPubMed
Machado J, Grimont F, Grimont PA. Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Res Microbiol. 2000;151:535–46. DOIPubMed
Bonanno L, Loukiadis E, Mariani-Kurkdjian P, Oswald E, Garnier L, Michel V, et al. Diversity of Shiga toxin–producing Escherichia coli (STEC) O26:H11 strains examined via stx subtypes and insertion sites of Stx and EspK bacteriophages. Appl Environ Microbiol. 2015;81:3712–21. DOIPubMed
Shringi S, Schmidt C, Katherine K, Brayton KA, Hancock DD, Besser TE. Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157. PLoS One. 2012;7:e51572. DOIPubMed
Delannoy S, Mariani-Kurkdjian P, Bonacorsi S, Liguori S, Fach P. Characteristics of emerging human-pathogenic Escherichia coli O26:H11 strains isolated in France between 2010 and 2013 and carrying the stx2d gene only. J Clin Microbiol. 2015;53:486–92. DOIPubMed
Mariani-Kurkdjian P, Lemaître C, Bidet P, Perez D, Boggini L, Kwon T, et al. Haemolytic-uraemic syndrome with bacteraemia caused by a new hybrid Escherichia coli pathotype. New Microbes New Infect. 2014;2:127–31. DOIPubMed
Table. Virulence gene profile of the stx2d-positive Shiga toxin–producing Escherichia coli O157:H7 strain CNM-2140/12
Technical Appendix. Alignment with reference strains 06–5231, 5905, C165–02, and B2F1 of the amino acid sequences of the C-terminal end of the A subunit and the N-terminal end of the B... 219 KB
DOI: 10.3201/eid2308.170570
1These authors contributed equally to this article.


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Keeping up with the digital natives
Neil Hitchcock - Navitas
Paris Cowan | April 17, 2015
Neil Hitchcock
Forget about MOOCs, for Navitas CIO Neil Hitchcock the real digital threat is coming from a customer base that can't remember a time before Facebook.
It might be hard to believe, but just a few years ago, analysts and insiders alike were predicting the downfall of the university system at the hands of massive open online courses.
These days, the fire has all but gone out of the MOOC hype.
In California, UCLA was recently forced to absorb a US$7 million loss from its investment into an 'all digital' campus it had hoped would attract students from around the world. Elsewhere, studies have shown that online learners underperform against their face-to-face peers.
Navitas CIO Neil Hitchcock has 19 years of experience under his belt at the multinational educational provider, and has watched the tides come and go for the education industry.
He says he doesn't think MOOCs are in "any way whatsoever" a game changer for the education sector - and never will be.
Hitchcock offered four points to back up his argument: one, there is no economic value in a MOOC - "at some stage even a university that gets government funding needs to have some sort of revenue coming in," he says.
Two, MOOCs have huge drop out rates - up to 93 percent of starters.
Three, universities are instrumental in the transition from being a child to being a self-determining adult, he says, something you can't get from a MOOC.
And finally, the issue of accreditation - "how do you prove that person who has taken that assessment or who has been doing that work is in fact the person who signed up?"
However, Hitchcock doesn't see the concept of open online learning as completely useless.
"They are a marketing opportunity - they can attract 10,000 or 100,000 people and get a brand out there. They are also an opportunity for institutions to test their online content," he says.
"A lot of universities now are using them as a quick way to change their course materials to be more meaningful in a face-to-face context."
So what is the real disruption shaking up Navitas?
"It is our students," Hitchcock says.
"Unlike other workplaces where you might have a range of people who are 15 to 70 buying a service, we have a bunch of digital natives who expect to do things differently."
It means Navitas needs to be able to offer everything on a mobile device.
"This is a big disruption in education where there are still old legacy learning management systems that only show data on a nice 1024 x 750 screen. They've got to change."
The CIO knows this customer base is not going to tolerate the kind of clunky, siloed services that are often the hallmark of an ageing back-end.
His team is therefore currently working on an integration platform that will connect the enterprise across its 120 colleges and operations in 27 countries.
Based on IBM's Websphere enterprise service bus, Hitchcock expects the new back-end will allow him and his team to add and remove new applications "almost like lego blocks" without losing the continuity of student information required to seamlessly manage their study and enrolment.
"It is perhaps one of the largest things that we are doing right now," he said.
"From the point a person is interested in enrolling right through to when they become a a student, to when they're going on to become alumni, each of these different stages use a different management system.
"What is important is that we use the integration platform to make sure this whole experience is still seamless.
"It also gives us the ability to use best of breed tools for any part of the process. We don't have to be tied to old legacy systems that demand we use the whole stack."
Hitchcock is also readying the foundations for the day that education providers using data analytics to tailor the learning process becomes standard practice.
"The paradigm we have got at the moment says a student has to be in class for 14 weeks a semester, and must do a certain number of credit hours to be certified. It is a really old paradigm," Hitchcock said.
"Education providers are starting to question whether we should be putting 35 people in a room, and telling them to sit there for 14 weeks and learn content.
"We have the tools now to analyse assessments so we can see what that student doesn't understand and automatically send them down an alternative learning path. We can say 'you didn't really understand that concept, let's revisit it in a little more depth'."
Already at some of the Sydney colleges operated by Navitas, work is being done with the local learning management systems to leverage assessment and progress data to guide students through their study, but Hitchcock concedes that the work is still in an "embryonic stage".
He does, however, foresee this sort of bespoke approach to higher education, where students are allowed to complete courses at their own pace, as the inevitable path the sector is going down - much more of a sure thing than the MOOC-led apocalypse ever was.

redpajama_set_name: RedPajamaCommonCrawl


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Opinion: New Pope a 'Rebel with a Cause'
Argentine Cardinal Jorge Mario Bergoglio, selected as the new head of the Catholic Church, will be a Pope who embraces a conservative doctrine in theory, yet not necessarily in practice, at least based on his time as a cardinal in Argentina.
Tony Castro, in an opinion piece at Voxxi.com, writes, "Pope Francis may not look it. But make no mistake, he is a 76-year-old rebel – he is a Jesuit, after all — and on Wednesday he became a rebel with a cause: Making the church relevant again and bringing it into the 21st century. And as an Argentine, he brings a tradition immersed in a heritage that is uniquely Hispanic and that has influenced the history and culture of the Americas."
The new pope, Castro says, is officially against gay marriage but not necessarily actively opposed. "It has been on Bergoglio's watch that Argentina became the first Latin American country to legalize gay marriage, while its president, Cristina Fernandez, promoted free contraception and artificial insemination. Bergoglio couldn't stop that tide of modern civilization, and you have to wonder if he really tried despite his rhetoric against such contemporary developments as gay adoptions," Castro wrote.
Pope Francis is also on the record as criticizing the church's clerics. Castro wrote about a speech the new pontiff made where he "accused fellow church officials of hypocrisy for forgetting that Jesus Christ bathed lepers and ate with prostitutes." According to Castro, Pope Francis said, "In our ecclesiastical region there are priests who don't baptize the children of single mothers because they weren't conceived in the sanctity of marriage," preferring instead to lose the women and their children as followers. "These are today's hypocrites," the Pope said in that speech, "Those who separate the people of God from salvation."
Photo (c) The Vatican


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Praosiri Charusalaipong
Marketing Praosiri Charusalaipong
Praosiri, from Thailand, is a Marketing postgraduate research student at Strathclyde. Find out below what she enjoys most about studying here and what she hopes to do after her studies.
Why did you choose Strathclyde for your postgraduate research study?
I chose Strathclyde because of the reputation of the University and my department (Marketing). I had been to Glasgow many times from when I studied my Masters degree in England. I do love Glasgow and Scotland!
What is the nature of your research?
My research is in the area of service marketing and dark tourism. I study the experience offerings and visitor experiences in war-related sites such as battlefields.
What's the Strathclyde research community like?
The research community is not only the interaction among colleagues in the same department. We also engage in other departments in our Business School. And sometimes we try to help other researchers from other schools, such as being an interviewee or a respondent in their studies. We also setup an online Facebook page to share our studies or knowledge which we think it's useful for any research.
What are the Strathclyde facilities like?
The facilities at the University are awesome. In the library, I can find any articles and books I want or even use the inter-library loan service. The sports centre is excellent. All the staff are friendly and helpful. The IT service is good as well... very helpful.
How do you find the support from your supervisor and the wider Strathclyde team?
I meet my supervisors regularly and also contact via emails. For other things I need, I go to McCance Building or send an email to the international student team.
What's the best thing about Strathclyde?
'The place of useful learning' I love this sentence and it's definitely true. I enjoy the campus location, it's near everything in Glasgow.
What would you like to do after your PhD?
I plan to find a full-time job in Glasgow and remotely work for my family business in Thailand.
Find out more about our PhD programme and the areas of research on offer in the Department of Marketing.
We've a thriving international community at the University of Strathclyde, with students coming here to study from countries across the world. Find out more.


Yay, this website is safe. There's a kitemark and everything. What's not plugged in?
The UK's digital minister Ed Vaizey has floated the idea of adding kitemarks to websites that have strong security measures in place, following the attack on TalkTalk's business last week.
In many cases, businesses set out extremely detailed terms and conditions, but the idea that they are consumer-friendly is wide of the mark.
If I can take, as it were, the spirit of her question [Labour MP, Gisela Stuart], some kind of kitemark to denote companies that have robust cyber-security procedures in place would be something worth exploring.
Vaizey, a politico who is big on self-regulation, seemed to be suggesting that such a badge would allow customers to think that the website they are using poses no or little security risk.
But offering Brits such a false sense of security, as the fallout from the TalkTalk attack continues, is at best naive and at worst a total failure to adequately jump to the defence of consumers – the real victims in this whole mess.
'Misinformation' on government views on encryption? Fancy that!
During the debate, Vaizey also responded to questions about encryption, after he was asked if companies should use such technical safeguards to help secure customer data.
"It has to be said that companies should encrypt their information. There has been some misinformation that the government are somehow against encryption," the minister said, without elaborating further.
Over the weekend, TalkTalk boss Dido Harding stated that her company was under no legal obligation to encrypt customer data, much to the chagrin of the budget telco's customers.
The Information Commissioner's Office told The Register on Monday that current UK data protection law spelled out clear rules to companies handling customer info.
"All organisations must have appropriate security measures in place to prevent the personal data they hold being accidentally or deliberately compromised. Any measures put in place should prevent security breaches or limit the damage if they do occur," we were told by the data watchdog.
The Register has created a timeline of TalkTalk's contradictory comments following on from the initial announcement of a website outage.


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Would A Travel Ban Save More People From Ebola?
By Jessica BerardinoBy Mike Pintek October 6, 2014 at 4:39 pm
Filed Under:Dr. Amesh Adalja, Ebola, Jessica Berardino, Mike Pintek, travel ban
Doctors in protective gear work on March 31, 2014, inside the medical humanitarian group Medecins Sans Frontieres (Doctors without Borders) isolation ward in the southern Guinean town of Gueckedou. Aid organisation Doctors Without Borders said on March 31 an Ebola outbreak suspected of killing dozens in Guinea was an 'unprecedented epidemic' as Liberia confirmed its first cases of the deadly contagion. Guinea's health ministry this year has reported 122 'suspicious cases' of viral haemorrhagic fever, including 78 deaths, with 22 of the samples taken from patients testing positive for the highly contagious tropical pathogen. AFP PHOTO / SEYLLOU(Photo Credit: SEYLLOU/AFP/Getty Images)
PITTSBURGH (NewsRadio 1020 KDKA) – A UPMC doctor believes through all the knowledge he's obtained and his work experience, the Ebola virus cannot become an airborne virus.
Dr. Amesh Adalja can say wholeheartedly and with confidence that this virus is not as contagious as everyone is acting it is. He also adds you cannot contract the virus easily.
Dr. Adalja is a UPMC Infectious Disease Physician and Senior Associate at the UPMC Center for Health Security says that if this virus were to become airborne like depicted in the movie, "Hot Zone," it would come with a give and a take.
"Even if it could become airborne it would be a trade off probably between the way it transmits and how deadly it is," said Dr. Adalja. " So it's not a one for one thing that's all of a sudden going to be airborne and be a super virus, I don't think that's possible, and even if it was we wouldn't be getting the same disease."
Dr. Amesh Adalja Part 1
(Photo Credit: ThinkStock)
If Pittsburgh was to received our first Ebola patient, he believes that UPMC is ready. He described a detailed manual given to their hospitals describing everything that they would need to do to treat and take care of the patient. The instructions begin with the patient's first arrival, and even into disposing of his waste.
He adds how important it is to include your travel history with every doctor's visit. He said that is just as important as telling the doctor what allergies you have. For people who are traveling in and out of the country or even state to state can contract a unique virus to that area and might bring it back with them.
Now with the possibility of closing the transportation and quarantining victims of Ebola, there rises the possibility of people lying when they are questioned about their travel before they are cleared to travel home. But the doctor assures the public that the question portion is just one part and there are extensive physical examines as well.
Dr. Adalja stresses that Ebola is not going to spread like an epidemic and there won't be a general concern about it. He adds that a travel ban would be worse than better for containing the virus. This is because you have to attack the virus from it's source, so people and equipment need to be able to get in and out of that area. If you stop travel you could crumble their infrastructure.
"You have to remember that people are scared of Ebola, it is not very contagious, it's only spread through blood and body fluids, a travel ban will end up doing more harm than good," said Dr. Adalja. "And in the end it may paradoxically make the outbreak worse because we won't be able to get resources there and you will actually isolate that country and make it a dead zone."
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Logan Correctional Center is located in Lincoln Illinois. It is a multi-custody level female institution that can house up to 2,067 female offenders. This facility focuses on the rehabilitation of inmates through education and skill building, as well as supporting the importance of family relationships. Inmates are encouraged to participate in adult basic education classes, pre-GED courses, and to obtain their GED while incarcerated.
Vocational activities provided to build an inmate's skills include cosmetology, hair braiding, nail technologies, horticulture, culinary arts, and a variety of construction disciplines. Correctional industries are available at Logan Correctional Center that include working in a recycling shop and the helping paws program that allows inmates to work with animals. A large number of programs are available to female offenders in a wide array of subjects from domestic violence, sexual assault and abuse, to anger and stress management and substance abuse.
General population inmates have visiting everyday from 9am-8pm. Processing of visitors stops at 6:30pm so you must arrive before this time to enter into visitation. Visits have a maximum visit duration of four hours, however if space is not available or overcrowding becomes an issue the visits may be shortened. On weekdays if space permits visitation may be extended past four hours.
Segregation inmates have visiting Monday thruogh Friday only from 9am-2pm. You must arrive and be processed by 12:30pm or you will not be allowed to visit. All visits are one hour in length. You can visit a segregation inmate on weekends and holidays but must have advanced prior approval from the administrative officer.


Thank you for visiting my galleries. Here you will find categorized photos from different shoots. Just let your mouse hover over the word "GALLERIES" in the menu above to begin exploring the memories I've captured!


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Adolf Matteoschat (* 1. September 1866 in Kaltecken, Kreis Tilsit, Preußisch Litauen; † 18. Oktober 1947 in Landskrona, Schonen) war ein deutsch-schwedischer Industrieller.

Leben
Matteoschat besuchte ein Gymnasium in Tilsit, wahrscheinlich das Tilsiter Realgymnasium. Nach dem Abitur studierte er an der Albertus-Universität Königsberg Chemie. Im Sommersemester 1887 wurde er wie Richard von Schaewen Mitglied des Corps Masovia. Als Inaktiver wechselte er 1889 an die Albert-Ludwigs-Universität, wo er Verkehrsgast bei Rhenania Freiburg war. 1891 wurde er in Freiburg zum Dr. phil. promoviert. Erste Anstellungen fand er 1891/92 in der Zuckerfabrik in Schonowitz bei Zülz in Oberschlesien und im (chemischen) Handelslaboratorium in Breslau. An der Schlesischen Friedrich-Wilhelms-Universität betrieb er mikroskopische Untersuchungen. Im oberschlesischen  Kruppamühle war er 1892–1894 Chemiker in der Fabrik, die für die Kaiserliche Marine Schießwolle herstellte. Er zog dann nach Schweden und war 1894–1900 Betriebsingenieur bei den Schießpulverfabriken Skånska bomullskrutfabriks AB und AB Svenska krutfaktorierna in Annelöv bei Landskrona. Ab 1900 war er dort Betriebsleiter.  Seit 1919 schwedischer Staatsbürger, wurde er 1920 als Vorstandsmitglied berufen. Er wohnte in Dösjebro, Kävlinge.

Anmerkungen

Einzelnachweise

Chemiker (19. Jahrhundert)
Chemiker (20. Jahrhundert)
Industrieller
Corpsstudent (19. Jahrhundert)
Absolvent der Albert-Ludwigs-Universität Freiburg
Deutscher
Schwede
Geboren 1866
Gestorben 1947
Mann

redpajama_set_name: RedPajamaWikipedia


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Hwang Yongdae
Warrant Officer Hwang Yongdae was born in Iryeong-ri, Jangheung-myeon, Yangju-gun, Gyeonggi-do, Sep. 10, 1929. He was assigned to 1st Company, 1st Regimment, Tiger Division and was dispatched to Vietnam; stationed in the area of Binh Dinh Province. Warrant Officer Hwang Yongdae died Jul. 2, 1970 in Cat Nhon(BR 997445), from wounds caused by a booby trap explosion. He was buried at 106-ㅅ-058 graveyard in Seoul National Cemetry.
Cho Namchun
Sergeant Cho Namchun was born in Palseong-ri, Saenggeuk-myeon, Eumseong-gun, Chungcheongbuk-do, Apr. 9, 1946. He was assigned to 1st Company, 1st Regimment, Tiger Division and was dispatched to Vietnam; stationed in the area of Binh Dinh Province. Sergeant Cho Namchun died Mar. 6, 1967 in Phu Cat, Cat Hung (CR 032575), from wounds caused by a booby trap explosion during a road reconnaissance. He was awarded the Order of Military Merit Hwarang. He was buried at 02-3-3208 graveyard in Seoul National Cemetry.
Park Yongwoo
Sergeant Park Yongwoo was born in Dae-u-ri, Sangwol-myeon, Nonsan-si, Chungcheongnam-do, Jan. 1, 1948. He was assigned to 1st Company, 1st Regimment, Tiger Division and was dispatched to Vietnam; stationed in the area of Binh Dinh Province. Sergeant Park Yongwoo was killed Jul. 19, 1970 in Phu Phong(BR 731573) during an military operation. He was awarded the Order of Military Merit Hwarang. He was buried at 02-3-3207 graveyard in Seoul National Cemetry.
Kim Yeongjong
Staff Sergeant Kim Yeongjong was born in Mok-dong, Yeongdeungpo-gu, Seoul-si, Apr.23, 1946. He was assigned to 1st Company, 1st Regimment, Tiger Division and was dispatched to Vietnam; stationed in the area of Binh Dinh Province. Sergeant Kim Yeongjong was killed Jul. 20, 1970 in Phu Cat(BR 729568) during a search action. He was awarded the Order of Military Merit Hwarang. He was buried at 02-3-3205 graveyard in Seoul National Cemetry.
Kim Hosu
Staff Sergeant Kim Hosu was born in Hajapo-ri, Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Jul. 4, 1947. He was assigned to 1st Company, 1st Regimment, Tiger Division and was dispatched to Vietnam; stationed in the area of Binh Dinh Province. Staff Sergeant Kim Hosu was killed Jul. 20, 1970 in Phu Cat(BR 729568) during a search action. He was buried at 02-3-3198 graveyard in Seoul National Cemetry.


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Wouldn't you like sometimes to just give up? I would. I do. All of the time. When all is said and done what is left at the end of the day when you go into deep sleep but the Absolute? Another word for the Absolute is Consciousness.
We can call it Absolute Consciousness. It's where we came from. We knew it as babies. One day we will return to it. But we can also know it now – all of the time. It's the ultimate high, the deepest rest and our final destination.
Absolute Consciousness is very accessible. It's merged within the cells of your body, the thoughts in your mind and your everyday awareness. If you relax just a bit, lose a few layers of holding patterns and allow your senses to widen then you can feel this Consciousness. Loosen up just a bit more and you will be deep within. Eventually you will fully merge and you will not go anywhere else. There is nothing quite like home. Infinity, eternity and absolute merging are your home.
Come as you are and leave quite different. Attend the live event if you can but if not book the MP3 or Replay.
The MP3 & streaming Replays of the Sunday Live Transmissions will be around 2 hours in duration.


text:
Texas A&M snaps Rice's win streak to ring in New Year
By Cheryl Coward
Photos © Robert Franklin, all rights reserved.
No. 24 Texas A&M (10-4) rang in the New Year snapping Rice's six-game winning streak with an 80-57 victory.
The Aggies came out hot on a 27-9 run. Led by Kelsey Bone, who earned her fifth double-double of the season, the Aggies made 11 of their first 12 shots. They scored 49 points in the first 20 minutes for a new season high in first half points. Rice (8-5) had 30 at the break.
"The offense in the first 15 minutes was as good as I've seen it all season," said Texas A&M head coach Gary Blair. "We had all five players contributing. It's starting to come together hopefully at the right time. I'm pleased with our effort. Bone and (Karla) Gilbert were terrific inside."
Rice did not roll over in the second half and used a 12-4 run to get within 15 but that was the closest they would get to Texas A&M.
In addition to Bone, three other Aggies were in double figures. Kristi Bellock and Gilbert finished with 12 points each. Adrienne Pratcher scored 11 points and dished out nine assists.
Jessica Kuster led the Owls with 18 points. Megan Palmer scored 15 points and grabbed four rebounds.
The Aggies will return to Reed Arena to open SEC play against Alabama on Thursday. The Owls return to the court on January 10 at East Carolina, to open C-USA play.
Tags: Adrienne Pratcher, Gary Blair, Jessica Kuster, Karla Gilbert, Kelsey Bone, Taj McWilliams-Franklin


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