text:
Middleton & Tinsley Law Firm, PLLC
Bankruptcy for Business Debtors
Credit Card Debt Attorney
720 Credit Score
Divorce, and Alimony
Will and Estate Matters
Tupelo, Mississippi Child Custody Lawyer
There are 2 types of child custody under Mississippi law: physical and legal custody. Physical custody concerns who the minor child will live with. Legal Custody grants a party decision-making rights concerning, among other things, medical, educational, and welfare choices for the child.
Courts award custody according to the best interest of the minor children as follows:
physical and legal custody to both parents jointly;
physical custody to both parents jointly and legal custody to either parent;
legal custody to both parents jointly and physical custody to either parent;
physical and legal custody to either parent.
Joint physical custody means the parties share actual custody of the minor child and assures the child frequent and continued contact with both parents.
Joint legal custody means the parents are to exchange information concerning the health, education and welfare of the minor child, and to confer with one another in making decisions for the child.
When physical custody of children is awarded solely to one parent, the non-custodial parent usually is awarded rights of visitation. Most non-custodial parents receive regular overnight visitation as follows:
alternating weekends during the school year;
two or more consecutive weeks during the summer;
alternating holidays; and
one-half of Thanksgiving and Christmas holidays
The award of visitation to the non-custodial parent depends on the unique circumstances of each case and may differ greatly based on the distance the parties live apart from each other and other factors.
Contact us today to speak with a family law attorney about your needs and concerns. We're here to help with:
Child Support.
Don't wait another minute. Set up an appointment for a free consultation today.
At Middleton & Tinsley Law Firm, we understand that one of the most difficult matters in a divorce, separation, or pregnancy is determining who gets custody of the children and what type of visitation is appropriate. This is a deeply emotional matter and it is best to seek guidance from a lawyer. You do not have to be a perfect parent or have been a perfect spouse to receive custody, and often people give up rights because they are so entangled in their situation and do not understand the legal standard for determining custody rights. Let us help you think clearly and determine what is in the best interest of you and your children.
Middleton & Tinsley Law Firm, PLLC
POST OFFICE BOX 3129 TELEPHONE: 662-205-4749
TUPELO, MS 38803-3129 FACSIMILE: 662-269-2424
 TEXT: 662-586-1164
Physical Address: 2604 W. Main St, Suite C, Tupelo, MS
Content copyright © 2015. dm@mlawms.com. All rights reserved.


text:
Aspiring NYC Model Found Brutally Killed in Bushes in Caribbean
Desiree Gibbon was set to return from the island nation on Thursday, according to her family
By Wale Aliyu	• Published November 30, 2017 • Updated on December 1, 2017 at 2:32 pm
Desiree Gibbon, an aspiring NYC model, was visiting the island of Jamaica to scout talent in the area for a friend, family said
The 26-year-old's body was found on the side of a blood-caked road, in the bushes, Saturday morning; her throat had been slit
Gibbon, the oldest of three, had traveled to the country on Oct. 20 and had a 3-month visa to stay in the country; she was due home Thursday
An aspiring actress and model from Queens who was visiting the island of Jamaica was found dead with a slit throat on a rural road a few miles from the country's biggest resort town, according to the woman's family.
Desiree Gibbon's mother and aunt told News 4 New York that the 26-year-old's body was found on the side of a road in Anchovy, a small town near Montego Bay, on Saturday morning.
The Daily News, which first reported Gibbon's death, said when her body was found in the bushes off the road, it was caked with blood. Gibbon's family told News 4 that police said her throat had been slit and it appeared she had fought with her attacker.
"I'm hoping that in her fight she has obtained some DNA, whether under her fingernails, on her body or her teeth, somewhere," said her mother, Andrea Gibbon.
Family said they found out about Gibbon's death after authorities showed her photo to a family member, who owns the Montego Bay hotel where she was staying.
Family members said she was planning on scouting talent in the area for a friend in New York, but she was killed before she got that chance.
It's not clear why she was killed and no arrests have been made in the case.
"God is my witness, whoever did this is going to pay," said the 26-year-old's aunt, Peggy Brunner.
Gibbon, the oldest of three girls, had traveled to the country on Oct. 20 and had a three-month visa to stay in the country, according to the family. The West Virginia University graduate had been working as a bartender in Jamaica to save up to go back to school to become a documentary filmmaker, but was set to come home on Thursday.
Gibbon's family now has to travel to the Caribbean island to retrieve her body. They left Friday morning to catch a flight to the country. Gibbon's mother says an autopsy on her daughter may not be performed until next week and may take even longer to issue a death certificate, which is a necessary step in the process of bringing her body home.
"She's currently being kept at a funeral home," her mother said. "We'll go there. I don't know how that will be. My understanding is that she was beat up pretty bad."
She attended St. Francis Preparatory School and Cardozo High School in Queens and graduated from WVU with a degree in broadcast journalism.
The 26-year-old's aunt created a GoFundMe page to help pay for funeral expenses and to have Gibbons' body brought back to New York City. So far, almost $20,000 has been contributed to the cause.
New YorkNew York CityDarren PriceJamaicaCaribbean

redpajama_set_name: RedPajamaCommonCrawl


text:
From the Institutes
New fluorescent probes to image live cells with super-resolution microscopy
Fluorescent probes for imaging live cells
Scientists introduce new molecular labels for super-resolution microscopy
Researchers in the Department of Chemical Biology at the Max Planck Institute for Medical Research in Heidelberg have developed a new strategy for synthesizing cell-permeable and fluorescent probes for multi-color super-resolution microscopy in live cells. The so-called MaP (Max Planck) probes enable the fluorescence labeling of proteins in living cells with minimal background and excellent specificity, making them powerful tools to study biological phenomena on a molecular level.
Live cells incubated with four different probes and imaged directly without any washing steps.
© MPI for Medical Research
Probes used in life-cell fluorescence imaging must be able to penetrate cells and bind specifically to the protein of interest. Ideally, such probes also increase their fluorescence intensity when binding to their target as this increases the signal to noise ratio. The latter property is called fluorogenicity, as the probe becomes (more) fluorescent when binding to its target. Kai Johnsson and his group specialize in the search for such molecules, and now introduce a generally applicable strategy that represents a major advance in the quest for fluorogenic probes for live-cell imaging.
"Our strategy was to enhance and tune the properties of existing fluorescent probes to increase fluorogenicity and cell permeability," says Lu Wang, first author of the paper and a postdoc in the department of Kai Johnsson at the Max Planck Institute for Medical Research. Lu Wang initially focused on 6-TAMRA, a well-known rhodamine derivative. This fluorophore exists in a hydrophilic and fluorescent zwitterion 'open' form and a hydrophobic 'closed' form that is non-fluorescent but can penetrate cells. However, the equilibrium for regular 6-TAMRA greatly favors the fluorescent and zwitterionic open form and 6-TAMRA derivatives generally show low cell permeability.
By introducing electron-deficient amides into 6-TAMRA, Wang was able to shift the equilibrium towards the closed form, to maximize permeability, while binding to the target shifts the equilibrium back to the fluorescent open form, resulting in fluorogenicity. These new probes, dubbed MaP (Max Planck) probes, showed vastly improved performance in live-cell imaging relative to the widely-used 6-TAMRA derivatives. Motivated by these results, Wang and his colleagues applied this simple strategy to improve the performance of various fluorescent probes that target different structures and proteins in living cells. The resulting MaP probes span the entire spectrum of the visible light and can, for example, be used for live-cell imaging of cytoskeletal proteins such as actin, tubulin and intermediate filaments as well as other cellular structures such as mitochondria or nuclei with confocal as well as super-resolution microscopy.
"A crucial benefit of the new MaP probes is that their high fluorogenicity allows their use without any washing steps and that we have an entire palette of colors available," says Lu Wang. To highlight these features, the scientists in the Department of Chemical Biology conducted an experiment where live cells were incubated with four different probes and imaged directly without any washing steps, providing detailed insights into the dynamic behavior of proteins and cellular structures. "We are excited to provide the scientific community with a new tool to study important biological questions", says Wang. While applying their new MaP probes for applications in live-cell imaging, he and his colleagues will also continue to use their expertise in synthetic chemistry to design other probes for visualizing the central molecules of life.
Prof. Dr. Kai Johnsson
Director, Department of Chemical Biology kai.johnsson@mr.mpg.de
Dr. Lu Wang
Postdoc, Department of Chemical Biology lu.wang@mr.mpg.de
Elisabeth Fuhry
Press Officer +49 6221 486-650 elisabeth.fuhry@mr.mpg.de


Hot or cold, asparagus soup is a spring thing! Our creamy soup celebrates one of the most precious vegetables in a way that is comforting and healthy at the same time.
A main course salad done in the style of Nice that can be adapted to any season. Compose simple ingredients with powerful flavours for an elegant meal that's healthy and satisfying!
Slow browning vegetables is a fuss-worthy culinary technique that releases their natural sugar leaving sweet nutty flavour. Harmony is created in this soup with earthy mushrooms and bright lemon.
Galette is any round or free-form tart enfolding sweet or savoury fillings. Distinctly elegant, this easy rustic pie is a great choice when you find yourself with a bounty of peak season produce.
Elevate the ubiquitous banana bread from bit player to star! Ours is uber healthy while keeping the spongy, moist texture of the regular variety. Feel good about having this ideal snack anytime.
There is no better winter treat than this insane gnocchi classic! These tiny dumplings yield sublime flavour and pillowy texture beyond all pasta plus they double as tatters. Gnocchi is a labour of love and shows anyone you serve them to that they are special.
Cauliflower is all the rage for pseudo starchy creations of the comfort foods we crave. From pizza crust, rice & cream sauces to mashed potatoes, there is no limit to what cauliflower ninjas do with this versatile vegetable. Its mild flavour provides cooks with a blank slate… be a palate pleaser with our gourmet cauli-risotto!
Brussel sprouts are the cilantro of vegetables – you either love them or leave them. Maybe knowing that they are high in protein and fibre will coax your taste buds to come around? Give this combination a whirl.
Assemble your breakfast muffins ahead and bake in the morning for a laid back brunch! They're oh so delicious, excellent protein and perfect for feeding a crowd without much effort.


text:
Elton John, Sting, James Taylor etc. to perform at Rainforest Fund Benefit
25 February, 2012 admin News 0
The lineup for this year's Rainforest Fund benefit concert has been announced, and the list of artists set to perform at New York City's Carnegie Hall is an impressive one.
The performers at the Revlon-sponsored concert include Sting, Elton John, James Taylor, Meryl Streep, Katharine McPhee, Bryn Terfel, Rosanne Cash, Vince Gill and a number of surprise guests.
The Rainforest Fund, founded by Sting and Trudie Styler in 1989, came to life because of a direct request from a Kayapo Indian leader in Brazil attempting to protect his land.
"Sting and I founded the Rainforest Fund after seeing with our own eyes the destruction of one of the world's most precious resources," Styler said. "We're so thankful that artists like Elton John, James Taylor and the many others who are doing their part to support the Rainforest Fund in its quest to raise awareness of the global environmental crisis we're facing. If we all take action, we can help save the planet and prevent further harm to the rainforests and indigenous people who live there."
The Rainforest Fund benefit concert will be held on April 3, 2012.
Source: TheHuffingtonPost.com
PARTICIPATE TO SEE STING
Stanley Clarke and Stewart Copeland


In the not-so-ordinary events in our lives we are called and claimed by the Lord. Like Esther, may we find the courage to answer the call from the Lord. Esther 9.
Praise the Lord, for in our most dire moments, when even our earthly friends may abandon us, He is there for us. Ps. 124.
Wisdom does not interpret the world through rose-colored glasses. Wisdom's vision is straightforward, clear and honest. James 5.
It is especially good to be a part of the family of God. The family of God is grand. When we observe someone who is doing good in Jesus' name, we should rejoice and be glad.
Jesus addresses an expansive, universalistic view of the church. As long as something is being done in the name of Christ they will "by no means lose the reward." Mark 9:41.
Make them days of feasting and gladness, days for sending gifts of food to one another and presents to the poor. Esther 9:22.
Our help is in the name of the LORD, who made heaven and earth. Ps. 124:8.
The prayer of the righteous is powerful and effective. James 5:16.
Whoever is not against us is for us. Mark 9:40.
may love be ours, O Lord.
A song of Love and Compassion, a Song to Set Me Free.
God is My Rock of Salvation, a Beacon for My Soul.
Praise to the Rock and the Well Spring, Creator of My Soul!
Our Help is in the Name of the Lord. Ps. 124 Song. Esther Mui.
the justice and unity of your realm. Amen.
and to those who wander from the truth.
Sunday Sept. 27 – Blood Moon Community Celebration!
FREE Family Activities, Food, FUN!
Saturday Sept. 26 – Treasure Hunt Community Celebration!
What is the best way to describe faith? How does this differ from fear, and what do we mean by "fear" anyway?
The Lord in His World sees the concepts of honor and shame differently than those of us rooted in the material worlds around us. How do we reckon the two, ideal and real?
What character traits make the important woman in our lives so essential?
The key to greatness is to have a servant's heart. Let us have a heart like Jesus.
Whoever wants to be first must be last of all and servant of all. Mark 9:35.
Whoever welcomes one such child in my name welcomes me, and whoever welcomes me welcomes not me but the one who sent me. Mark 9:37.
A capable wife is far more precious than jewels. Prov. 31:10.
To you alone will my spirit yield.
I don't know exactly how sweet heaven will be (will be) I don't know what beauty or what glory I'll see (I'll see) I don't know what I'll behold that morning divine (I only) I know for sure that heaven's really gonna shine It's gonna shine.
Christians have several days within the Liturgical Year to reflect on the Cross used in the Crucifixion of Jesus as representing an Instrument of Salvation. Holy Cross Day has a rich history that dates back to the Crucifixion. This Feast Day is named Yψωσις τοῦ Τιμίου καὶ Ζωοποιοῦ Σταυροῦ in Greek, Воздвижение Креста Господня in Russian, Exaltatio Sanctae Crucis in Latin, Exaltation of the Holy Cross in the Roman Missal, Triumph of the Cross, and Feast of the Glorious Cross.
Lutherans classify this day as a "Lesser Feast Day." We call it Holy Cross Day. We focus on the Gospel message that Jesus Christ grants us the freely given Gift of Salvation through His Death on the Cross for Our Sins. It is not our choice to accept salvation. Salvation is granted to us in the belief that Jesus Christ is our Savior. This belief finding its basis in Scripture is called "Justification." This is our focus on Holy Cross Day.
I take this opportunity to explore our belief in salvation and its loss further. Our salvation is not dependent upon on our good works, although we freely engage good works that are pleasing to our Lord. We aspire to do our best to live good lives, for when we commit sin and persist in sin, we are choosing to turn away from God. This act of persistent turning away from God will result in loss of salvation. The sinner must repent and believe in Christ to be saved. Martin Luther. Lutheran Confessions. Galatians Lectures. Catechism. Smalcald Articles. Book of Concord.
Whoever believes in Him may have eternal life. For God so loved the world that he gave his only Son, so that everyone who believes in him may not perish but may have eternal life. Indeed, God did not send the Son into the world to condemn the world, but in order that the world might be saved through him. John 3:15-17.
In the cross our need meets God's redemption In confidence and hope, we bring the prayers of our hearts for the transformation of the church and the world. Amen.
Sing to the Lord a New Song. Psalm 98.
Accept our prayers, God of mercy, in union with Jesus the Christ, who offered his life for our salvation upon the wood of the cross and who now reigns with you in eternal glory forever and ever. Amen.
I hear the Savior say, "Thy strength indeed is small; Child of weakness, watch and pray, Find in Me thine all in all." Jesus paid it all, All to Him I owe; Sin had left a crimson stain, He washed it white as snow.
For nothing good have I, Whereby Thy grace to claim, I'll wash my garments white, In the blood of Calv'ry's Lamb.
Prayer: Through suffering and rejection, O Lord, you bring forth our salvation, for in Jesus you embrace our humanity and transform our lives by the glory of his cross. Grant that for the sake of the gospel we may rebuke the lure of this world, take up our cross, and follow your Son Jesus Christ. Amen.
Although Peter had just moments before declared Jesus as the Christ, he turned from God's perspective and viewed the situation from man's perspective, which brought about the stern rebuke: "Get behind me, Satan!" Jesus goes on to explain: "You do not have in mind the things of God, but the things of men." Mark 8:33.
Jesus' stern reprimand did not make sense to Peter. However, Jesus' indictment presents a profound message for us. We can easily see that Peter has the wrong perspective of God's plan for Christ's suffering and death. We must also see how easily we can become an unwittingly spokesperson for Satan. This is especially true when we lose sight of God's plan for us. This comes about when our main and ultimate focus is on our careers, our possessions, our security, the things of the world rather than upon sacrifice and service and the proclaiming of God's message. When Peter's focus shifted to his own desires and plans, Jesus rebuked him in order to get him back on track. May our focus always be on God and His plans, that we may never experience a similar rebuke from our Lord. Amen. – Got Questions Ministry.
Get behind me, Satan! Mark 8:33.
God of Justice, Savior to all, Came to rescue the weak and the poor; Chose to serve and not be served.
Jesus, You have called us, Freely we've received, Now freely we will give.
We must go, live to feed the hungry, Stand beside the broken, we must go.
Prayer: O Lord, Wisdom of the universe, you bear the pain of your people. Grant us the gift of wisdom, that we may discern your way and live justly and graciously amid the struggles of this world. Amen.
Prayer: Move us to praise your gracious will, for in Christ Jesus you have saved us from the deeds of death and opened for us the hidden ways of your love. We ask this through Jesus Christ our Lord. Amen.
Prayer: Redeeming Sustainer, visit Your people and pour out your strength and courage upon us, that we may hurry to make you welcome not only in our concern for others, but by serving them generously and faithfully in your name. Amen.
Till we meet till we meet God be with you till we meet again.
Daily I still provide you God be with you till we meet again.
New Horizons "My life is well-rounded and I am becoming a more comfortable version of myself, not the neurotic, boring person that I thought I'd be without drugs." JFT. N.A. Page 266.
Just for Today: I can live a well-rounded, comfortable life, a life I never dreamed existed. Recovery has opened new horizons to me and equipped me to explore them.
(C) 2007. NA Narcotics Anonymous World Services, Inc.
And watch it drown in new horizons, New Horizons.
Cheering and laughing at the dying, While we're riding the line in You.
Addiction is an attractor field, a low vibrational level.
to that highest level to which You call me.
that You call me loudly.
and into a higher level attractor field.
and because you love me so very much.
And so it is. Amen. – Prayerforce.
Every time I get close to a rosebush or a bouquet of flowers, I am unable to resist the temptation to pull a flower toward my nose to savor the fragrance. The sweet aroma lifts up my heart and triggers good feelings within me.
Writing to the Christians in Corinth centuries ago, the apostle Paul says that because we belong to Christ, God "uses us to spread the aroma of the knowledge of him everywhere." 2 Cor. 2:14. Through His strength we can live a victorious life, exchanging our selfishness for His love and kindness and proclaiming the goodness of His salvation. When we do this, we are indeed a sweet fragrance to God.
Paul then switches to a second image, describing Christians as a "letter from Christ." 2 Cor. 3:3. The letter of our lives is not written with ordinary ink, but by the Spirit of God. God changes us by writing His Word on our hearts for others to read.
Both word pictures encourage us to allow the beauty of Christ to be seen in us so we can point people to Him. He is the One who, as Paul wrote in Ephesians 5:2, "loved us and gave himself up for us as a fragrant offering and sacrifice to God." – Lawrence Darmani. Daily Bread.
Lord, let Your splendor fill my life, that I may draw people to You. Help me walk in the way that spreads the fragrance of Your love to others. Amen.
Our actions speak louder than our words. Amen.
We asked His protection and care with complete abandon. – ALCOHOLICS ANONYMOUS, p. 59.
Only then did I start growing! God forgave me. A Higher Power had to have saved me, because the doctors doubted that I would survive. I have forgiven myself now and I enjoy a freedom I have never before experienced. I have opened my heart and mind to Him. The more I learn, the less I know, a humbling fact, but I sincerely want to keep growing. I enjoy serenity, but only when I entrust my life totally to God. As long as I am honest with myself and ask for His help, I can maintain this rewarding existence.
Just for today, I strive to live His will for me, soberly.
Today, life is beautiful! – A.A. Daily Reflections, September 8.


Cooking demos are held in a classroom style which consists of a lecture and cooking demonstration and followed by a food testing. Our chefs will also share techniques and helpful hints to prepare delicious and beautiful food in a way that even the average cook will find useful.
Min 10 pax in a group required per demo session. Call us at 604-8811 797 Ext 303 for more information.


text:
				<?php print_r($con); ?>

				 Consignments

				00,000

				 Assigned

				<?php print_r($cus); ?>

				 Registrations

				<?php print_r($car); ?>

				 Carriers

redpajama_set_name: RedPajamaGithub


text:
| Katalog
| Hintergrund
This disc is noted for the all-tube equipment in the recording chain up to the point when the analog signals captured initially by the Neumann M49 tube microphones (the music) is converted to digital and normalized to the so-called Red Book CD Standards so as to be played as regular CDs. It should be noted that these classic Neumann M49 tube microphones were developed in 1949 and manufactured around 1951. From the top, to my ears this orchestra just got rid of all romanticized vibrato and played with pure tone, and as a result the instruments sound much older than they really are. The music's execution thus shows a small chamber orchestra becoming in touch with the past; it is a modern instrument orchestra that sounds like a period instrument ensemble. Changing to period bowing styles makes a world of difference and there is no question about it, the tempi are fast and there is nothing ponderous in these enlightened performances. On this disc nobody gets in the way of Vivaldi, nor interferes with him either, and all seem to be Vivaldi's advocates!
The Four Seasons from the Opus 8 is a unique set of four violin concertos by Antonio Vivaldi (1678-1741) composed in 1723 and first published as scores for solo violin and string quartet in 1725. The present version is a modern transcription with different instrumentation for a small chamber orchestra including a harpsichord providing the basso continuo part. These four concertos were written by Vivaldi to go along with four sonnets and they are, therefore, true tonepoems, and an early example of programmatic music. Do yourself a favor and follow the music with the text of the sonnets and see how much more sense the whole thing makes. The text for the sonnets can be found at www.baroque-music-club.com/vivaldiseasons.html
On listening to this Four Seasons there is a physical sensation that can, as in this recording, be transformed into an emotional sensation that comes through the sounds and the expert manipulation of stringed instruments' acoustics; instrumental acoustics is not the same as hall acoustics. Instrumental acoustic balancing control is exemplary throughout but none more so than in Tracks No. 8 (Adagio molto), No. 14 (Adagio) and No. 17 (Adagio). Moreover, the strings are supremely strong and precise throughout while observing a warm pure tone as in Track No. 10's an Allegro molto violin solo with cellos and contrabass accompaniment which in turn moves swiftly to the marvelous violin pizzicatos of Track No. 11's Largo with their perfect syncopation, and the remaining strings providing pure tonal harmony.
The music in this disc is not about anyone, not even about Daniel Gaede - the violin soloist - the music is about Vivaldi with a very lean and taut sound. The present orchestra jells together very well their modern instruments and period sound with their lilting fast allegros, sharply-executed phrasing in the slow middle movements, and the joyous final prestos. There is energy and commitment from the orchestra's parts, the soloist and the conductor as evidenced by their precise entrances and of all things no discernible mistakes. All this I am saying about the Four Seasons can be transposed to the other two violin concertos on this disc just as well.
The "tubes only" sound is adequate but nothing on the spectacular side; I am not convinced that "tubes only" regular CDs have much to contribute and I would rather see (or hear) a "tube only" SACD. On this CD imaging is excellent but dynamics are just rather flat, save for an occasional entrance of the cellos and/or the contrabass when playing very loud. My very subjective opinion is that an SACD recording would have been a much better choice. In this "tube only" incarnation the sound is less than…
Final words: fortunately the artistry of the performance amply surpasses the just average sound and that could be a good enough reason to justify this disc.
John Nemaric


An adult deer can eat more than six pounds of food every day. So if your food plot isn't producing, your coveted animals will go elsewhere. But planting a food plot isn't always simple, and many who want to attract deer and other critters are hunters as opposed to farmers.
Long story short, it's important to make it as easy as possible to create a productive food plot. Enter Ragan & Massey's PlotSpike® line of high-quality seed blends. From cold-tolerant to early season, shady blends to no-till varieties, there is a PlotSpike product that will help your food plot, and the animals it attracts, thrive.
But don't take our word for it. Here's evidence of PlotSpike in action.
Jason Langdon of the Northern Adirondack Outdoorsman's Page tells of his PlotSpike experience. Jason used our PlotSpike® Clover Blend and Quick Stand varieties. Of Clover Blend he says, "Six days since planting it, with 100% wall-to-wall germination." Quick Stand, meanwhile, offered complete coverage in three and a half weeks. Neither variety was planted in ideal conditions: "This was all during a drought season, plus it's in high elevations and in the middle of the woods," Jason says of his thriving food plot in northern New York.
See Jason's original post here!
All Ragan & Massey seeds are sourced, produced and packed by Ragan & Massey. We know the origin of our seeds and the genetic capabilities of our varieties. With several varieties developed exclusively for our customers, we are confident that our pure, proven and premium seeds work, with no fillers or unnecessary coatings. That's why all our seeds for food plots are 100 percent guaranteed.
Click below to find the PlotSpike seed that is right for your food plot.


text:
Q: Verilog file naming question I just started to learn Verilog in college so please forgive me if this is an elementary question. I know there can be multiple modules in one .sv file. But in that case, what is the naming convention for that file? For example, if I have a Verilog file with module Test1 and Test2, what should I name this .sv file? Because normally when the professor demonstrates writing Verilog there is only one module in each file so he just uses the module name for the file name.

A: Most Verilog compilers have an automatic search capability that look for modules that you have instantiated but have not compiled yet. It will take the name of the module and append .v to the filename for Verilog files and .sv for SystemVerilog file. Then search through specified directories for that filename. You do need to be careful of file compilation order dependancies with timescales and macros.
Whether you use that capability or not, it is a good file naming convention.

redpajama_set_name: RedPajamaStackExchange


Thornton's Bakehouse & Butchers is a third-generation family-run Company owned by John Thornton who lives in Coxwold and is the son of Geoff of Thornton's Butchers of Easingwold, which stood in Easingwold market place.
Thornton's specialises in handcrafted pork pies, pastries and high-quality local meats. They offer a full bakery and butchery service to wholesale and retail customers alike. Orders can be made by calling 01347 868 190 and orders can be delivered free locally or collected from Husthwaite. Treat us as you would your local Butchers!
The bakery produces hand-made pies and pastries baked daily in Husthwaite. Thornton's takes great pride in producing delicious, hearty, traditional products which taste as good as they look, and which uphold the exceptional Thornton's reputation developed over decades of serving our customers. The Company also offers unique Bespoke Pork Pies which are perfect for a special present or celebration.
Thornton's Butchery has been in the family for generations and has developed strong relationships with local farmers and suppliers. They continue to be passionate about providing local meat bred in North Yorkshire.
The Fauconberg Arms in the village often features Thornton's products on their menu.
Katie Turner of Thornton's runs the Coxwold Village Market on the last Saturday of every month, where Thornton's is a permanent feature. She also attends the quarterly Husthwaite Village Market and the Sherriff Hutton Village Market on the third Sunday of every month.


text:
Q: Spark Cassandra Connector has bad read performance, produces a lot of disk writes by kworker Some followups at the bottom
I have a test installation of Spark and Cassandra where I have 6 nodes with 128GiB of RAM and 16 CPU cores each. Each node runs Spark and Cassandra. I set up my keyspace with the SimpleStrategy and a replication factor of 3 (i.e., fairly standard).
My table is very simple, like this:
create table if not exists mykeyspace.values (channel_id timeuuid, day int, time bigint, value double, primary key ((channel_id, day), time)) with clustering order by (time asc)

time is simply a unix timestamp in nanoseconds (the measuring devices the values come from are that precise and this precision is wanted), day is this timestamp in days (i.e., days since 1970-01-01).
I now inserted about 200 GiB of values for about 400 channels and tested a very simple thing - calculate the 10-minute average of every channel:
sc.
  cassandraTable("mykeyspace", "values").
  map(r => (r.getLong("time"), r.getUUID("channel_id"), r.getDouble("value"))).
  map(t => (t._1 / 600L / 1000000000L, t._2) -> (t._3, 1.0)).
  reduceByKey((a, b) => (a._1 + b._1) -> (a._2 + b._2)).
  map(t => (t._1._1 * 600L * 1000000000L, t._1._2, t._2._1 / t._2._2))

when I now do this calculation, even without saving the result (i.e., by using a simple count()) this takes a VERY long time and I have a very bad read performance.
When I do top on the nodes, Cassandra's java process takes about 800% CPU, which is OK because this is about half the load the node can take; the other half is taken by Spark.
However, I noticed a strange thing:
When I run iotop I expect to see a lot of disk read, but I see a lot of disk WRITE instead, all of which comes from kworker.
When I do iostat -x -t 10, I also see a lot of writes going on.
Swap is disabled.
When I run a similar calculation directly on the CSV files the data came from, which are stored in HDFS and loaded via sc.newAPIHadoopFile with a custom input format, the process finishes much faster (the calculation takes about an hour with Cassandra but about 5 minutes with files from HDFS).
So where can I start troubleshooting and tuning?
Followup 1
With the help of RussS (see comments) I discovered that logging was set to DEBUG. I disabled this, set logging to ERROR, and also disabled GC logging, but this did not change anything at all.
I also tried keyBy as the very same user pointed out, but this also did not change anything.
I also tried doing it locally, I tried it once from .net and once from Scala, and here, the database is accessed as expected, i.e., no writes.
Followup 2
I think I got it. For once, I didn't see the forest for the trees, because the hour I stated earlier for 200GiB is still about 56 MiB/s throughput. Since the hardware I run my installation on is far from optional (it is a high performance server which runs Microsoft HyperV which in turn runs the nodes virtually, and the hard disks of this machine are quite slow) this is indeed a throughput I expect. Since the host is just one machine with one RAID array where the disks of the nodes are virtual HDDs, I can't expect the performance to magically go through the roof.
I also tried to run Spark standalone which improves the performance a bit (I now get about 75 MiB/s), and also the constant writes are gone with this - I only get occasional spikes I expect because of shuffling.
For the CSV files being much faster, the reason is that the raw data in CSV is about 50 GiB and my custom FileInputFormat that reads it, does it line by line, and is also using a very fast string-to-double parser which only knows the US format but is faster than Java's parseDouble or Scala's toDouble. With this special tweaking I get about 170MiB/s in YARN mode.
So I suppose I should, for once, improve my CQL queries to limit the data that gets read, and try to tweak some YARN settings.


The Lyme disease spirochete Borrelia burgdorferipossesses 15 silent vls cassettes and a vlsexpression site (vlsE) encoding a surface-exposed lipoprotein. Segments of the silent vls cassettes have been shown to recombine with the vlsE cassette region in the mammalian host, resulting in combinatorial antigenic variation. Despite promiscuous recombination within the vlsE cassette region, the 5′ and 3′ coding sequences of vlsE that flank the cassette region are not subject to sequence variation during these recombination events. The segments of the silent vlscassettes recombine in the vlsE cassette region through a unidirectional process such that the sequence and organization of the silent vls loci are not affected. As a result of recombination, the previously expressed segments are replaced by incoming segments and apparently degraded. These results provide evidence for a gene conversion mechanism in VlsE antigenic variation.
Borrelia burgdorferi, the agent of Lyme disease, possesses an elaborate genetic system, designated vmp-like sequence (vls), on a 28-kb linear plasmid (lp28-1) (41). The presence of lp28-1 correlates with the high-infectivity phenotype in strains of B. burgdorferi, and homologous plasmids are present in low-passage, infectious strains of the Lyme disease spirochetes Borrelia afzelii and Borrelia garinii (41). Thevls system has been characterized in B. burgdorferi B31 clone 5A3 (B31-5A3) (41), and in this strain consists of a vls expression site (vlsE) located near the right telomere of lp28-1 and 15 silent vlscassettes upstream. vlsE encodes a surface-exposed lipoprotein with a predicted molecular mass of 34 kDa. The coding sequence of vlsE in B31-5A3 contains a vlscassette region in the middle and two stretches of 5′ and 3′ flanking sequences which will be called noncassette regions hereafter. The central vlsE cassette region is separated from the noncassette regions by a 17-bp direct repeat sequence, which also separates the 15 silent vls cassettes (41).
The vlsE cassette region of B31-5A3 has up to 92% DNA sequence identity with the silent vls cassettes. The 15 silent cassettes begin ∼200 bp upstream of vlsE and are oriented in the opposite direction, away from the telomere. They form a nearly contiguous, 8-kb open reading frame interrupted by only one stop codon and two frameshifts, but they lack promoter sequences and apparently are not expressed. The silent cassettes are 474 to 594 bp in length, and each is delimited by the same 17-bp direct repeat found in the vlsE expression site (with the exception of the 5′ and 3′ ends of the entire silent cassette locus). In this manner, thevls silent cassettes closely resemble the middle portion ofvlsE.
Most sequence differences among the vls cassettes are confined within six highly variable regions (41). DNA segments of the silent cassettes are able to recombine in an apparently random manner into the vlsE cassette region in C3H/HeN mice throughout the course of infection (41, 42). Sequence results are consistent, with roughly 6 to 11 recombination events with multiple silent vls cassettes during the first 28 days of infection (41). The promiscuous recombination events at thevlsE site lead to extensive genetic and antigenic variation in VlsE variants.
A recent publication by Kawabata et al. (16) shows that a similar, yet divergent, vls system exists in B. burgdorferi 297. Comparisons of patient and tick isolates from New York also indicate that the vls sequences of some strains closely resemble those of B. burgdorferi B31-5A3, whereas others are quite different (14). The sequence information available for comparison is incomplete at this point, and further analysis will be needed to provide a more complete picture of the heterogeneity of the vls system among Lyme disease isolates.
The vls system resembles a previously characterized genetic system encoding surface-exposed variable major proteins (VMP) in the relapsing fever agent Borrelia hermsii (2).B. hermsii has at least 26 vmp genes on multiple linear plasmids; only 1 vmp gene, located at avmp expression site near one end of a linear plasmid, can be expressed by a single organism at a given time (2). Recombination events between the expressed and silent vmpgenes lead to antigenic variation and thus evasion of the host immune response during the course of mammalian infection (3, 25,28).
Antigenic variation of surface-exposed proteins has been identified as an important immune evasion mechanism in a number of additional pathogenic bacteria and parasites. In most cases, antigenic variation results from gene conversion events between the expressed genes and silent or nonexpressed genes or copies (5, 35). For example, nonreciprocal replacement of the expressed vmp sequence by silent vmp genes is the primary mechanism for vmpantigenic variation in B. hermsii (2), although other mutations and recombination events can also occur (29,30). A similar mechanism appears to be responsible for recombination between the expressed pilin gene (pilE) and silent copies (pilS) in Neisseria gonorrhoeae(8, 10, 43). Antigenic variation of variable surface glycoprotein (VSG) in African trypanosomes also results in part from gene conversion events in which the silent vsg genes recombine into the telomeric expression sites (5).
In the present study, we analyzed the vlsE and silentvls cassette loci of B. burgdorferi M1e4A and M1e4C (41) obtained from a C3H/HeN mouse infected 28 days previously with the parental strain B31-5A3. The results indicate that the silent vls cassette locus and the vlsEnoncassette regions are preserved during the course of vlsEvariation, findings consistent with a gene conversion mechanism in which segments of the silent vls cassettes replace corresponding regions in the vlsE expression site.
Spirochete strains. B. burgdorferi B31 clone 5A3 (B31-5A3) was isolated from the infectious low-passage strain B31 and identified as a high-infectivity strain by Norris et al. (27). B31-5A3 was previously used to characterize thevls system and vlsE antigenic variation (41). B. burgdorferi clones M1e4A and M1e4C were isolated from a single ear biopsy specimen from a C3H/HeN mouse infected 28 days previously with B31-5A3 (Fig.1) (41). The spirochetes were cultured in BSK II medium as described previously (26). Ear biopsy isolate 1396 was obtained from a C3H/HeN mouse 28 days postinfection with M1e4C; clone 1396D was cultured from isolate 1396 by subsurface colony plating (27).
Derivation of B. burgdorferi B31 clones in C3H/HeN mice. Clone B31-5A3 was the parental strain used in this study. Clones M1e4C and M1e4A were cultured from a single ear biopsy from a C3H/HeN mouse at 28 days postinfection with B31-5A3 (41). Similarly, clone 1396D was derived from a skin biopsy of a mouse inoculated with 105 M1e4C 28 days previously.
PCR techniques.All PCR amplifications were carried out in a Minicycler thermal cycler (MJ Research, Watertown, Mass.) with the Thermalase PCR kit (Amresco, Solon, Ohio). All primers were obtained from GenoSys Biotechnologies, Inc. (Woodlands, Tex.) and dissolved to a final concentration of 100 μM in H2O as stocks. The entire coding regions of vlsE alleles were amplified by PCR by using forward (+ strand) primer F4224 and reverse (− strand) primer R4225 at a final concentration of 20 μM (Table 1). Primers F4224 and R4225 are located at the 5′ and 3′ noncoding regions ofvlsE, respectively (41).
The silent vls cassette sequences in B. burgdorferi M1e4C and 1396D were PCR amplified with vlscassette-specific primers at a final concentration of 20 μM. The sequences and combinations of these primers used are described in Table1 and in the Results section, respectively. B. burgdorferi plasmid DNA was prepared according to the method of Hinnebusch et al. (12) and used as a template. The PCR parameters utilized included 35 cycles of denaturation at 95°C for 40 s, with annealing at 64°C for 40 s followed by extension at 72°C for 2 min in a reaction volume of 100 μl. The final cycle was followed by an additional extension at 72°C for 5 min.
Southern hybridization. B. burgdorferi plasmid DNA was prepared with stationary-phase organisms as previously described (12). B. burgdorferi total DNA was prepared with stationary-phase organisms by proteinase K digestion and phenol-chloroform extraction according to the method of Walker et al. (40). B. burgdorferi DNA was digested with restriction enzymes, separated by agarose gel electrophoresis, and transferred to nylon filters as described previously (41).
Oligonucleotides R4232, F4234, F4289, and R4290 were used as probes for Southern hybridization. The locations and sequences of these oligonucleotides are shown in Fig. 2 and Table 1, respectively. Portions (30 pmol) of oligonucleotides were radiolabeled by using T4 polynucleotide kinase (Promega, Madison, Wis.) and [γ-32P]ATP (Amersham Life Sciences, Arlington Heights, Ill.) in a total volume of 25 μl by standard methods (1). The radiolabeled probes were then purified through STE Select-D G-25 columns (5 Prime→3 Prime, Boulder, Colo.) and hybridized with the B. burgdorferi DNA blots at 65°C for 16 h as described previously (41). The blots were washed sequentially with 0.5% sodium dodecyl sulfate in 2× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) at 65°C for 30 min, 1× SSC at 65°C for 30 min, and 0.1× SSC at room temperature for 20 min (34). Autoradiography was carried out by using X-Omat film (Kodak, Rochester, N.Y.) with enhancing screens.
Nucleotide sequence comparison between the parentalvlsE allele (vlsE1) and two derivative alleles,m1e4A and m1e4C. Identical nucleotides and gaps are marked as dashes and dots, respectively; nonidentical nucleotides are shown as letters. The 17-bp direct repeats flanking thevlsE cassette region are shaded. The predicted ribosome binding site (RBS), putative start codon (M), and putative stop codon (∗) are indicated. Locations and directions of oligonucleotides used in this study are marked with arrows.
DNA sequence analysis.PCR products were purified by using Wizard columns (Promega, Madison, Wis.), and the remaining salts were removed in Microcon-100 columns (Millipore, Bedford, Mass.). DNA sequences were determined with an ABI 377 DNA sequencer (Perkin-Elmer/ABI, Foster City, Calif.) at the University of Texas—Houston Microbiology and Molecular Genetics DNA Core Facility. Sequences were analyzed with Genetics Computer Group (Madison, Wis.) programs as previously described (41). PILEUP and BOXSHADE programs were used to produce graphic output of sequence alignments.
Nucleotide sequence accession numbers.DNA sequences of thevlsE allele (vlsE1) and silent vlscassettes in the parental strain B31-5A3 are available under the GenBank entries U76405 and U76406, respectively. The complete coding sequences of vlsE alleles m1e4A andm1e4C are contained in the updated versions of U84554 andU84556, respectively. The vlsE cassette sequence of clone 1396D was deposited in GenBank under accession entry AF030082.
The 5′ and 3′ noncassette regions of vlsE remain unchanged.It has been shown previously that considerable sequence variation occurred within the vlsE cassette region ofB. burgdorferi during infection of C3H/HeN mice (41). However, the sequences outside the cassette region were not determined in variant clones, so it was not known whether the 5′ and 3′ noncassette regions of vlsE are affected during recombination events. To determine the sequences of the noncassette regions following infection in mice, we chose two B. burgdorferi clones, M1e4C and M1e4A, both of which were isolated from the same C3H/HeN mouse 28 days postinfection with the parental clone B31-5A3 (Fig. 1). The cassette sequences of vlsEalleles m1e4A and m1e4C have been previously determined (41).
The entire coding regions of vlsE for both strains were PCR amplified by using flanking primers F4224 and R4225 localized in the 5′ and 3′ noncoding regions (Table 1), and the PCR products were sequenced directly. Sequence analysis revealed that the 5′ and 3′ noncassettevlsE sequences for progeny clones M1e4A and M1e4C were identical to those of the parental strain B31-5A3 (Fig. 2). In contrast, both vlsE alleles exhibited numerous nucleotide sequence changes within the vlsE cassette region (Fig. 2), resulting in extensive changes in the predicted amino acid sequences (41). Thus, neither the 5′ nor the 3′ noncassette region is altered during vlsE cassette region recombination in the mammalian host.
The 5′ and 3′ noncassette regions of vlsE are present only at a single vlsE site.In a previous study, we identified a vlsE site in B. burgdorferi clone B31-5A3 by cloning and sequencing analysis (41). Multiplevsg expression sites have been shown in African trypanosomes, although only one of them appears to be expressed at a given time (5). To determine whether B. burgdorferi possesses multiple vlsE sites forvls recombination, the oligonucleotides F4289 and R4290 were used as markers of vlsE to probe the plasmid DNA blots ofB. burgdorferi B31-5A3, M1e4A, and M1e4C by Southern hybridization. Oligonucleotides F4289 and R4290 (Table 1) are located at the 5′ and 3′ noncassette regions of vlsE, respectively (Fig. 2).
In the parental strain and the two variants, only one major DNA band corresponding to vlsE was detected by both the 3′ probe R4290 (Fig. 3) and 5′ probe F4289 (data not shown). The sizes of the hybridizing fragments matched those predicted from the nucleotide sequence and were identical for the two probes except for the RsaI and Sau3AI digests, for which the probes are predicted to hybridize with fragments of different sizes. The presence of weakly hybridizing bands is most likely due to cross-hybridization with other unrelated sequences. These results indicated that the 5′ and 3′ noncassette regions ofvlsE are not present in other B. burgdorferiplasmids. Similar results were observed when using B. burgdorferi total DNA blots and the entire noncassette regions as probes (data not shown). In these experiments, the 5′ and 3′ noncassette regions were amplified by using primer sets of F4289-R4084 and F4219-R4225 and then used as probes (Fig. 2; Table 1). The extreme 5′ end of vlsE was not included in the probe because it is partially homologous to the 5′ end of the silent cassettevls2 (41). These results provide evidence that only one vlsE locus on linear plasmid lp28-1 is present in the parental strain B31-5A3 and the two progeny variants M1e4A and M1e4C. They also show that the region surrounding vlsE does not undergo extensive rearrangement during recombination, as demonstrated by the nearly identical patterns obtained with the parent strain and the two variants.
Detection of the vlsE noncassette sequences. Southern blots of restriction enzyme-treated plasmid DNA from the parental strain B31-5A3 and two progeny strains M1e4A and M1e4C were hybridized with oligonucleotide probe R4290, representing the 3′ noncassette region of vlsE. The molecular sizes of DNA bands are indicated in kilobases on the left side.
Segments of silent vls cassettes are duplicated into the vlsE site.Our previous study (41) indicated that segments of the silent vls cassette sequences replaced portions of the vlsE cassette region. However, the mechanism by which the segments recombined into the vlsEcassette region was not known. There are at least two possibilities involving reciprocal and nonreciprocal recombination mechanisms (35). Reciprocal recombination would result in exchange of sequences between the silent vls cassettes andvlsE. In this case, the sequences of the silentvls cassettes would be altered. Conversely, nonreciprocal recombination would allow duplication of the silent vlscassette sequences into vlsE, preserving the sequence and structure of the silent vls cassette locus. To test these possibilities, several oligonucleotide probes representing variablevls segments were hybridized with the blots of the plasmid DNA from the parental strain B31-5A3 and its derivative clones digested with restriction enzymes.
Sequence analysis revealed two sequences specific for the silentvls cassettes. The sequence corresponding to the 23-mer R4232 (Table 1) was present in the vlsE expression site of M1e4C but was found only in the silent cassette vls11 of the parental strain B31-5A3 (Fig. 2 and 4A), indicating that this vls11 sequence had recombined into thevlsE gene of M1e4C. Similarly, the sequence represented by the 30-mer oligonucleotide F4234 (Table 1) is present only in the variable region 3 (VR-III) of the silent cassette vls10(41). The specific region of vls10 represented by oligonucleotide F4234 had recombined into the vlsE cassette region of mouse isolate M1e4A (41). These probes thus served as specific markers of the vls11 and vls10sequences located either in the silent cassette loci or in both the silent cassette region and vlsE.
Evidence for unidirectional recombination of silentvls cassette segments into vlsE. (A) Diagram of locations of oligonucleotide probes in vlsE and the silentvls cassettes in the parental strain B31-5A3 and its derivative strains M1e4A and M1e4C. (B) Hybridization of oligonucleotide probe R4232 with the blots of restriction enzyme-digested plasmid DNA preparations from B31-5A3, M1e4A, and M1e4C. In all lanes, the probe hybridized to a single band corresponding to a region containing the silent cassettevls11 in both strains B31-5A3 and M1e4A. However, for strain M1e4C, two bands were detected in each digest that corresponded to thevlsE region with the second band (arrows). The molecular sizes of DNA bands are indicated in kilobases on the left side.
Based on the restriction sites present in vlsE and the silent vls cassettes, PstI, RsaI,Sau3A, and SpeI would produce restriction fragments for vlsE that are distinct from those of the silent vls cassettes. These enzymes were chosen to digest plasmid DNA from B31-5A3, M1e4C, and M1e4A for Southern blots. The blots were hybridized with radiolabeled oligonucleotides F4234 and R4232.
Only the silent cassette vls11 contains sequence of oligonucleotide R4232 in clones B31-5A3 and M1e4A (Fig. 2 and 4A); therefore, only a single band was detected for all of the lanes (Fig.4B). In contrast, in strain M1e4C, PstI, RsaI, and SpeI digestions yielded two hybridizing bands with the same probe (Fig. 4B). These results showed that strain M1e4C contains two copies of the probe sequence, one in vlsE and the other in the silent cassette vls11. Sau3AI digestion of thevlsE allele m1e4C was predicted to produce a 429-bp DNA fragment, while the Sau3AI fragment of the silent cassette vls11 is 489 bp in length. Therefore, the hybridization band in the Sau3AI lane in strain M1e4C presumably represented two comigrating DNA fragments (Fig. 4B). Similarly, the vls10-specific oligonucleotide F4234 hybridized with both vls10 and vlsE bands in the restriction pattern of strain M1e4A and with only the silentvls10 copy in B31-5A3 and M1e4C Southern blots (data not shown).
Except for the extra vlsE bands for recombinant strains M1e4A and M1e4C, the hybridization patterns for all three strains were identical, indicating that vls recombination does not result in gross DNA rearrangement in the silent vls cassette locus. Thus, the overall structure of the silent vls cassettes appears to be conserved during vls recombination. In terms of the recombination mode, segments of the silent vlscassettes appear to be simply duplicated into the vlsEcassette region. Therefore, a unidirectional recombination mechanism seems to be operative in vls recombination.
The sequence of the silent vls cassette locus is preserved.The oligonucleotide hybridization results described above suggested that the overall structure of the silent vlscassette locus is not altered during vlsE recombination (Fig. 4B). However, these hybridization experiments only showed the presence of these vls segments and did not provide further information about possible sequence changes in other regions of the silent vls cassette locus. Due to considerable sequence redundancy in the silent vls cassette locus, it is difficult to identify unique primer sets to amplify and verify the sequences of the silent cassettes by standard PCR methods.
We were able to PCR amplify and sequence portions of several silentvls cassettes in strain M1e4C and its variant 1396D, derived as shown in Fig. 1. A 697-bp region of vls5 was amplified by using primers F4265 and R4277 (Table 1). Similarly, primers F4234 and R4232 allowed us to amplify a 720-bp fragment covering the 3′ region ofvls10 and the 5′ region of vls11. Finally, a 249-bp region of vls11 was amplified with primers F4280 and R4279. These silent vls cassettes in strains M1e4C and 1396D were identical to those in the parental strain B31-5A3 in terms of DNA sequence (data not shown), although sequences from these regions had recombined into the vlsE expression site in these variant progeny. These results provide further evidence that the silentvls cassette locus does not undergo sequence variation during vlsE recombination.
The previously expressed vls segments are lost.The regions of the vlsE cassette that are replaced during recombination could be conserved by progeny. This could occur either by retention of lp28-1 plasmid copies within the same cell that did not undergo recombination or by transfer of those sequences to another, undefined site. In B. hermsii, an expressed vmpgene is degraded and lost when it is replaced by a silentvmp gene (17, 25, 28). To test whether the expressed vls segments are removed or retained in theB. burgdorferi genome after being replaced by othervls segments, oligonucleotide R4232 was radiolabeled and used to probe the restriction enzyme-digested total DNA of B. burgdorferi clone 1396D. Clone 1396D was derived from strain M1e4C during infection of a C3H/HeN mouse (42) (Fig. 1) and had lost part of the oligonucleotide R4232 sequence from thevlsE site.
In contrast to the presence of two copies of probe R4232 in the parental strain M1e4C (Fig. 4B), the same probe detected only a single band in each restriction digest in the progeny strain 1396D, representing the silent vls11 cassette in all lanes (Fig.5). These results indicated that the displaced vls segments from the vlsE locus are not preserved elsewhere in the B. burgdorferi genome, a finding consistent with a gene conversion and loss of the replaced sequences.
Loss of the previously expressed vls cassette segments. The total DNA blot of M1e4C-derived clone 1396D was hybridized with oligonucleotide probe R4232. In all lanes, the probe hybridized to a single band containing the silent cassettevls11. The molecular sizes of DNA bands are indicated in kilobases on the left side.
The results presented in this study have provided evidence for a gene conversion mechanism in vlsE genetic and antigenic variation. First, we showed that genetic duplication of vlssilent cassette segments into the vlsE expression site appears to be responsible for the extensive sequence variation within the vlsE cassette region (41) (Fig. 4B). Second, sequence and Southern blot hybridization analyses indicated that the sequence and organization of the silent vls cassettes were conserved during recombination in the three isogenic B. burgdorferi B31 strains examined. Finally, direct comparison of restriction patterns between the parental and progeny strains revealed that the vlsE cassette sequences are degraded and are not retained elsewhere in the genome following recombination events.
Programmed gene rearrangements (4) or genetic variation (31) have been described in both prokaryotic and eukaryotic organisms. The classic example of programmed gene rearrangement in eukaryotes is the V(D)J rearrangement and isotype switching that occur in immunoglobulin and T-cell receptor expression (7, 19). This process involves site-specific recombinases that recognize inverted nonamer and heptamer sequences at either side of the gene segment "joints" in the case of V(D)J rearrangement or switch-site specific sequences upstream of each constant region locus in isotype switching (19). Although the vls recombination process involves replacement rather than deletion, it is possible that the 17-bp direct repeats at either end of the expressed and silentvls cassette regions or other conserved sequences are involved in site-specific recognition by the putative recombinase(s) responsible for this activity.
Our studies thus far have suggested that B. burgdorferi has an elaborate system to ensure this unidirectional recombination. The unidirectional and segmental recombination features of vlsEantigenic variation in B. burgdorferi resemble those of the pilin antigenic variation in N. gonorrhoeae. N. gonorrhoeaepilin encoded by pilE is the main subunit of the surface-exposed pili, which have been shown to promote gonococcal infection (36). Segments of several silent pilin gene copies (pilS) scattered on the gonococcal chromosome can replace the existing sequences in pilE through unidirectional recombination events (8, 10, 43). The resulting changes in the amino acid sequence of the pilin protein can lead to antigenic variation (37) and variation in human tissue tropism (15). The molecular mechanisms of gonococcal pilin antigenic variation are still unclear, although the RecA protein has been shown to be essential (18). Homologous sequences in pilin genes have been shown to be important, including theSmaI-ClaI region (38, 39) and a conserved cys2 region (13).
A site-specific invertase responsible for inversion of a DNA segment and pilin phase variation in Moraxella lacunata has been characterized (22, 23). This invertase apparently recognizes specific 19-bp repeat sequences (32) at the inversion sites. A putative N. gonorrhoeae recombinase (gcr) was able to recognize the same M. lacunata sequences and to catalyze the pilin gene inversion in a surrogate Escherichia coli system, but it is still unclear whether gcr plays a role in gonococcal pilin variation or virulence (33). Gonococcal mutants deficient in pilin antigenic variation have been generated recently by transposon mutagenesis (24). These mutants had undetectable pilin gene recombination at thepilE expression site. Based on these observations, we believe that conserved sequences of vlsE and silentvls cassettes are necessary for vlsE antigenic variation and that one or more site-specific proteins are involved in the recombination process. The required sequences may include the 17-bp direct repeats that flank the cassette regions of vlsE and the silent vls cassettes. The genome sequence of B. burgdorferi (6) contains several plasmid-associated genes encoding putative "transposase-like proteins," but these genes most closely resemble transposases associated with insertion sequences and also contain frameshifts. Therefore, these genes are unlikely to encode the vls recombinase protein(s), and other candidate genes have not as yet been identified.
There are also notable differences between vlsE and gonococcal pilin antigenic variation systems. Unlike the silent pilin genes which are scattered across the gonococcal chromosome (9), the silent vls cassettes are organized in a compact, head-to-tail array in lp28-1 (41). In addition,N. gonorrhoeae is capable of taking up extracellular DNA, which has been implicated as a source for pilin antigenic variation (11). However, natural competence in DNA transformation has not been reported in B. burgdorferi, although lateral transfer of genetic information has been suggested based on the sequence heterogeneity of several B. burgdorferi genes (20, 21). These distinctions may reflect other differences in the molecular mechanisms of the two antigenic variation systems.
The 5′ and 3′ noncassette regions of three vlsE alleles examined did not vary in the mouse isolates examined, despite extensive sequence variation with the vlsE cassette region (Fig. 2). Site specificity of a proposed recombinase may be a factor in this respect. It is possible that both noncassette regions are required for an as-yet-unknown function of the VlsE protein, so that there is a selection pressure against sequence variation in these regions. Finally, lack of sequence variation in the noncassette regions may simply be due to lack of corresponding silent copies in the genome.
Like "germline" immunoglobulin and T-cell receptor loci of vertebrates, the silent vls cassette locus appears to serve as a stable reservoir for vlsE antigenic variation. By multiplying the number of possible amino acids at each variable position in the silent vls cassettes, the number of possible amino acid combinations has been estimated to be over 1030(unpublished data). This estimate assumes that recombination can occur at any location within the vlsE cassette and that all possible combinations are permissible for the survival and growth ofB. burgdorferi. Even with these potential limitations,B. burgdorferi may have a nearly inexhaustible capacity forvlsE sequence variation.
We thank Daimin Zhao, Jerrilyn Howell, John Hardham, and Dachun Wang for providing technical assistance and helpful suggestions.
This work was supported by grant AI37277 from the National Institutes of Health.
(1996) Current protocols in molecular biology. (John Wiley & Sons, New York, N.Y).
(1990) Antigenic variation of a relapsing fever Borrelia species. Annu. Rev. Microbiol. 44:155–171.
(1991) Variable antigen genes of the relapsing fever agent Borrelia hermsii are activated by promoter addition. Mol. Microbiol. 5:489–493.
(1987) Programmed gene rearrangements altering gene expression. Science 235:658–667.
(1995) Mechanisms of antigenic variation in Borrelia hermsii and African trypanosomes. J. Biol. Chem. 270:7783–7786.
(1992) Molecular analysis of V(D)J recombination. Annu. Rev. Genet. 22:425–446.
(1986) The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion. Cell 44:107–115.
(1992) Silent pilin genes of Neisseria gonorrhoeae MS11 and the occurrence of related hypervariant sequences among other gonococcal isolates. Mol. Microbiol. 20:197–208.
(1985) Intragenic recombination leads to pilus antigenic variation in Neisseria gonorrhoeae. Nature 315:156–158.
(1996) Limited variation and maintenance of tight genetic linkage characterize heteroallelic pilE recombination following DNA transformation of Neisseria gonorrhoeae. Mol. Microbiol. 20:507–518.
(1990) Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi. Mol. Microbiol. 4:811–820.
(1996) The size and position of heterologous insertions in a silent locus differentially affect pilin antigenic variation in Neisseria gonorrhoeae. Mol. Microbiol. 22:509–522.
(1998) Conservation and diversity among Borrelia burgdorferi isolates from upstate New York, abstr. D-139. Abstracts of the 98th General Meeting of the American Society for Microbiology 1998. (American Society for Microbiology, Washington, D.C), p 233.
(1994) Sequence changes in the pilus subunit lead to tropism variation of Neisseria gonorrhoeae to human tissue. Mol. Microbiol. 13:403–416.
(1998) Genetic and immunological analyses of Vls (VMP-like sequences) of Borrelia burgdorferi. Microb. Pathog. 24:155–166.
(1990) Juxtaposition of expressed variable antigen genes with a conserved telomere in the bacterium Borrelia hermsii. Proc. Natl. Acad. Sci. USA 87:6077–6081.
(1987) Effects of recA mutations on pilus antigenic variation and phase transitions in Neisseria gonorrhoeae. Genetics 117:391–398.
(1997) The origins of V(D)J recombination. Cell 88:159–162.
(1990) Identification, cloning, and sequencing of piv, a new gene involved in inverting the pilin genes of Moraxella lacunata. J. Bacteriol. 172:4370–4377.
(1988) Pilin gene phase variation of Moraxella bovis is caused by an inversion of the pilin genes. J. Bacteriol. 170:3032–3039.
(1997) Random shuttle mutagenesis: gonococcal mutants deficient in pilin antigenic variation. Mol. Microbiol. 23:1121–1131.
(1985) Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia. Cell 41:403–409.
(1992) Low-passage-associated proteins of Borrelia burgdorferi B31: characterization and molecular cloning of OspD, a surface-exposed, plasmid-encoded lipoprotein. Infect. Immun. 60:4662–4672.
(1995) High- and low-infectivity phenotypes of clonal populations of in vitro-cultured Borrelia burgdorferi. Infect. Immun. 63:2206–2212.
(1985) Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsii. Nature 318:257–263.
(1994) Antigen diversity in the bacterium B. hermsii through "somatic" mutations in rearranged vmp genes. Cell 78:867–876.
(1994) Activation of a vmp pseudogene in Borrelia hermsii: an alternate mechanism of antigenic variation during relapsing fever. Mol. Microbiol. 13:287–299.
(1992) Genetic variation in pathogenic bacteria. Trends Genet. 8:422–427.
(1991) Interesting sequence differences between the pilin gene inversion regions of Moraxella lacunata ATCC 17956 and Moraxella bovis Epp63. J. Bacteriol. 173:4000–4006.
(1997) Inversion of Moraxella lacunata type 4 pilin gene sequences by a Neisseria gonorrhoeae site-specific recombinase. J. Bacteriol. 179:2382–2388.
(1988) Genetic mechanisms of bacterial antigenic variation. Microbiol. Rev. 52:327–336.
(1989) Mechanisms for variation of pili and outer membrane protein II in Neisseria gonorrhoeae. in Mobile DNA. eds Berg D. E., Howe M. M. (American Society for Microbiology, Washington, D.C), pp 743–761.
(1983) Monoclonal antibodies to gonococcal pili: studies on antigenic determinants on pili from variants of strain P. J. Gen. Microbiol. 129:1965–1973.
(1994) A conserved DNA sequence is required for efficient gonococcal pilin antigenic variation. Mol. Microbiol. 13:75–87.
(1997) Analysis of protein binding to the Sma/Cla DNA repeat in pathogenic Neisseriae. Nucleic Acids Res. 25:1362–1368.
(1995) Physical map of the genome of Treponema pallidum subsp. pallidum (Nichols). J. Bacteriol. 177:1797–1804.
(1997) Antigenic variation in Lyme disease borreliae by promiscuous recombination of vmp-like sequence cassettes. Cell 89:275–285.
(1998) Kinetics and in vivo induction of genetic variation of vlsE in Borrelia burgdorferi. Infect. Immun. 66:3689–3697.


text:
Lampard agent confirms Chelsea summer departure
January 7, 2013 May 15, 2015 Andrew Tuft
The agent of Frank Lampard has confirmed that the Chelsea midfielder will definitely leave Stamford Bridge when his contract expires at the Premier League club, Sky Sports has reported.
It had been speculated in the Press over the weekend that Chelsea owner Roman Abramovich was having second thoughts as to the future of Lampard after the supporters' reaction to the prospect of the England international leaving the club, but Steve Kutner, Lampard's agent, has revealed that Chelsea have made their stance on Lampard clear.
"Chelsea executives told Frank in Japan during the Club World Cup then again reconfirmed with me after the Everton victory that in no circumstances will he be offered a new contract to stay at the club after the end of this season," Kutner said. Lampard scored both of Chelsea's goals in the 2-1 win at Goodison Park and was on target again in the 5-1 FA Cup victory at Southampton on Saturday, converting a penalty.
Kutner added: "Nothing since has changed in any respect. Frank has had to accept that and just wants to carry on playing football for Chelsea so as to finish the season as successfully as possible for the club that he loves."
Lampard has been at Chelsea since 2001, moving across London from West Ham United but has been linked with a switch to Major League Soccer side LA Galaxy.
See what the expert tipsters at OLBG are tipping on Stoke v Chelsea
Stoke Focus – Has the Michael Owen gamble failed to pay off?
Suarez criticised after handball goal in Liverpool FA Cup win
August 24, 2020 James Hill
Can Chelsea Be a Serious Title Contender Next Season?
Chelsea's season may have not ended the way Blues fans wanted but there is no denying...
June 10, 2020 James Hill
What Will Happen to Chelsea's Current Strikers if Timo Werner Signs?
This week, news came out that RB Leipzig striker Timo Werner has agreed personal terms with...
Chelsea Europe Premier League


text:
Hexatoma juliana är en tvåvingeart som beskrevs av Alexander 1937. Hexatoma juliana ingår i släktet Hexatoma och familjen småharkrankar.

Artens utbredningsområde är Kuba. Inga underarter finns listade i Catalogue of Life.

Källor

Småharkrankar
juliana

redpajama_set_name: RedPajamaWikipedia


text:
Scott H Brady, MS, MCP
Manager, Office of Travel Monitoring
The Office of Travel Monitoring conducts approximately 5,000 traffic counts per year, a combination of motorized vehicles and increasingly the bicycle and pedestrian non-motorized modes. Since adding bicycle and pedestrian counting to an arsenal of data collection tools, the program has established a network of permanent counting stations for use in seasonal factor development, project before / after counts for project evaluation, and most recently a cyclical bicycle counting program to provide a systematic method for tracking usage trends. Together with database and GIS staff, web viewers have been developed and advanced for the dissemination of count data to stakeholders and the public.
Beyond work at the commission, Mr Brady Chairs the Bicycle & Pedestrian Data Subcommittee of TRB and is a member of the Highway Travel Monitoring and Urban Transportation Data committees. He has led the bicycle / pedestrian topic tract on the organizing committee for the last two NATMEC conferences. He was a member of the FHWA Expert Panel which guided development of the latest edition of the Travel Monitoring Guide, the first to include a chapter on non-motorized data collection. He has also participated in an FHWA sponsored TPCB Peer Exchange.
His education includes a Master of Science in Transportation and a Master of City Planning, both from the University of Pennsylvania. He has a BA from the University of Washington.
Panel - Data Moves Government - Collecting, Using and Presenting Data to Advance Your Mission DATA & TECHLIMITED


How do they shoot those silky-smooth timelapse travelling shots? Or a precisely moving, gliding tabletop product shot?
Sliders are great, but they're only as smooth as the finesse with which you can operate them. For really smooth slider moves, the trick is to be sure you're using the new Ronford-Baker Motorised Slider.
Its controller is a zoom control-sized hand unit that you can program for feathered starts and stops.
The motor moves a 4-keyed Mitchell mount along the slider with a toothed belt at speeds from fast 1 ft/sec to slow 1 ft/min. You can add the Motorised Unit to existing Ronford Sliders, or order the complete set. It's available in lengths from 25" to 96", and custom lengths as well.
Easily fitted onto any Ronford-Baker slider.
Minimum height: 4 ¾" (12 cm).
Overall width of slider: 10" (25 cm).


text:
Tag: Stix hooper
LIGHTHOUSE 68″ ~ JAZZ CRUSADERS LIVE ! CELEBRATING 50 YEARS…STILL FUNKY FRESH
posted by Robert J. Carmack
The Lighthouse Café is a nightclub located at 30 Pier Avenue in Hermosa Beach, California. It has been active as a jazz showcase since 1949 and, under the name "The Lighthouse", was one of the central West Coast jazz clubs from the 1950s through the late 1970s. Purely talking Jazz years, It has been a long time, since I personally use to frequent these digs as a very young budding saxophonist growing up in the early 1960s Los Angeles. Listening to two jazz stalwarts on the radio at the time in KBCA FM 105.1 & the KNOB "JAZZ KNOB" out of Long Beach. hipster Jocks ruled the radio waves back then LA. Tommy Bee, Jammin' Jai Rich, Rick Holmes, Chuck Niles, Les Carter, Tollie Strode with "Slow traffic to the right" , "while travelin' in my Electro-Magnetic Bag."
AllMusic rated the album with 4 stars noting: "Feel is what dictates the material and its execution on this set, without unnecessary attention paid to crowd or recording apparatus. This is one the most intimate jazz shows captured on tape during the 1960s. It gives record buyers the sound of a band in full possession of their considerable capabilities, celebrating them in a relaxed environment, playing their own brand of grooved-out '60s jazz". I actually had the opportunity to catch this very lively and "Funky as it wanted to be band" ,to borrow a catch-phrase from the era describing the feel and sound of the times. Borrowing heavily from the fresh pop culture and songs the crusaders were able to marry the hard bop edginess and the approachable groove-based chart-topping style. Jazz Crusaders were starting to dominate the west coast appeal and the young fans like myself. I was all of 17 in the fall of 1967, when this was recorded, but released in early 1968.
The best part about growing up in the early 60s and loving live jazz , You could actually go see who was playing the music you heard on radio. Because of the policy for "under -21" fans who obeyed the rules and "maintained their cool" under the fire of the electrifying bands that appeared at the beach venue.. IMHO, You might as well considered the Crusaders the House band, they appeared hundreds of times over the years, but recorded almost all of their most classic of all records with that great Lighthouse crowd over the microphones too. All of which gave it that "Flavor" like a great Gumbo meal. I would be here all day trying review all the great music from the pen or horns of Jazz crusaders, starting in 1962 , one could catch the very best in music, bar none, and be on the "hippest scene" while doing it at the Lighthouse.
I invite you to pick up a copy of this great album or whatever choice of media format is today vinyl, CD or downloads. GET IT!!
Join me ,along with some very special musicians in Los Angeles June 22 Friday night at 9pm as we turn back the clock 50 years and perform the best of the jazz crusaders: the early years up to late 70s. I have culled simply the best compositions and recordings for the featured band Crusader Legacy 5 Plus.. hand-picked stellar musicians for this event at the World Stage Performance Gallery 4321 Degnan Blvd. L.A. California 90008 – $25 –
Go online at http://www.eventbrite.com under title of Street Life: The Magic and Music of the Jazz Crusaders ….
"Ooga-Boo-Ga-Loo" (Stix Hooper) – 6:39
"Eleanor Rigby" (John Lennon, Paul McCartney) – 7:32
"Native Dancer" (Buster Williams) – 8:52
"Never Had It So Good" (Joe Sample) – 7:15
"The Emperor" (Williams) – 8:50
"Impressions" (John Coltrane) – 6:12
"Cathy the Cooker" (Wayne Henderson) – 6:22 Bonus track on CD reissue
"Shadows" (Williams) – 4:03 Bonus track on CD reissue
"Tough Talk" (Henderson, Hooper, Sample) – 8:01 Bonus track on CD reissue
"Third Principle" (Wilton Felder) – 8:45 Bonus track on CD reissue
Wayne Henderson – trombone
Wilton Felder – tenor saxophone
Joe Sample – piano
Buster Williams – bass
Stix Hooper – drums
Posted on May 30, 2018 May 30, 2018 Categories HALL of SOUL, happenings, jazz event, Jazz Hall of Fame event, People to Watch, Poetry / LiteratureTags #blues2jazzguy, #robertjcarmack, avant Garde, Baby Boomer, blue music, composers, entertainment, festivals, Joe Sample, spiritual jazz, Stix hooper, straight ahead, travel, Wayne Henderson, Wilton Felder, world musicLeave a comment on LIGHTHOUSE 68″ ~ JAZZ CRUSADERS LIVE ! CELEBRATING 50 YEARS…STILL FUNKY FRESH
STREET LIFE: MAGIC & MUSIC JAZZ CRUSADERS ~ LIMITED DISCOUNTED TICKETS ON SALE NOW !!
CALL 951-840-7120 or email blues2jazz2003@yahoo.com Regular price is $25 online at http://www.Eventbrite.com
Special discounted price Save up to $7.25 off Online price including fees
Contact Robert regarding Direct sales Must pay Cash or money Order for 5 or more tickets @ $20 per ticket
Starting Limited period May 29th ENDS June 10th ONLY $20 per ticket when you buy (5)FIVE or more from one of our reps.
951-840-7120 for general info or closest rep to you.
Hipster Sanctuary.Com
Street Life: Magic & the Music of Jazz Crusaders
a Musical and Poetic Tribute
featuring The Crusader Legacy 5 Plus Band
Robert J. Carmack Poet, Teodross Avery Saxophones, Alvin Starks Trombone, Don Littleton Drums,Theo Saunders-Piano,Mike Alvidrez Bass
JUNE 22nd Friday Night – 9PM World Stage Performance Gallery 4321 Degnan Blvd. LA , Calif. 90008 $25 at http://www.eventbrite.com
Robert J. Carmack – Producer/Poet/Host
Teodross Avery
Alvin Starks
Don Littleton
Theo Saunders
Mike Alvidrez
Posted on May 30, 2018 May 30, 2018 Categories jazz event, Jazz Hall of Fame, People to Watch, Poetry / LiteratureTags #blues2jazzguy, #robertjcarmack, blue music, Buster Williams, California, classic jazz, Composer, entertainment, festivals, hard Bop, Jazz Crusaders, Joe Sample, Live at Light house Jazz Cafe, performance, Stix hooper, straight ahead, travel, Wayne Henderson, Wilton Felder, world music1 Comment on STREET LIFE: MAGIC & MUSIC JAZZ CRUSADERS ~ LIMITED DISCOUNTED TICKETS ON SALE NOW !!
COMING JAZZ APPRECIATION MONTH APRIL: TRIBUTE TO JAZZ CRUSADERS BAND & LEGACY
COMING TO LOS ANGELES ONLY
JAZZ APPRECIATION MONTH APRIL 2018
a Robert J. Carmack Production
Details February during Black History month, follow-up on www.hipstersanctuary.com as we celebrate our 20th year in March 2018. April Live Jazz concert and special awards presentation to surprise guests, also follow-up @blues2jazzguy.
Posted on January 29, 2018 Categories "HAPPENINGS", concerts, jazz event, Jazz Hall of Fame event, People to WatchTags #blues2jazzguy, #robertjcarmack, Afro-Carribean sounds, blue music, Blue thumb records, Buster Williams, California, Chisa, classic jazz, classic soul, composers, drums, entertainment, Hugh Masakela, Jimmy Bond, Joe Sample, Leroy Vinnegar, Old School music, Pacific Jazz, Stewart levine, Stix hooper, Wayne Henderson, Wilton Felder, world musicLeave a comment on COMING JAZZ APPRECIATION MONTH APRIL: TRIBUTE TO JAZZ CRUSADERS BAND & LEGACY
SOUTH AFRICAN MUSICIAN HUGH MASAKELA JOINS THE ANCESTORS-RIP 1939-2018
It seems that a bevy of greats have left the stage and building since January of 2017. I realize that's just life as we know it. Nothing to say about it case closed. However, I did not want to allow the sudden death of a great man and musician go by without saying anything about it. First, my exposure to Hugh Masakela goes back beyond 50 years(1966). I lived in Los Angeles and was studying music in high school and two off campus jazz bands too. Soon summer 1966 arrived and I was quite anxious because, word had it, the very first WATTS FESTIVAL was coming to reality. Heavy announcements of Music, Art and Pageantry to replace all the violence and melee that happened only one year prior.
The opening act was this new guy we had been hearing about from Africa that was making a lot of noise in New York.
Hugh Masakela was the Kick-off concert at Jordan High school gym that launched the 1966 Watts Festival & Cultural events. I can remember like yesterday as me and a group of guys who loved jazz, was quite excited about the possibilities and the fact it would be my first time seeing anyone from Africa that was not a cliché of Hollywood racists attitudes about portraying ,anyone from the motherland. That night was very special in more ways than the obvious. I was 16 and thought I was a grown man…the other was coming from a sociopolitical viewpoint. Black people were making a transition from being negro or colored people to Black people or Afro-American (first popped up as a description of black people at this time). Anyway, back to the music, Hugh was every bit an image and role model for us young men. he had a very interesting hair-style or "Natural", wore full African regalia, including "NO Shoes" as he went through the recently released album cuts of 1966 "The Americanization of Ooga-Booga." Which I know now, was a title given and sanctioned by the marketing department at the record company. I assure you, mine and most of us were concentrating on the musical style of his trumpet playing and the rhythms being crafted by the unit led by Hugh. Larry Willis on piano, Henry Jenkins on Drums, Henry Franklin on Bass and Percussionist Big Black pounding out the beats on African drums and Congas.
Hugh was a master of blending the American style of jazz bop and blues idioms juxtaposition with African Rhythms. The eclectic mix of originals showcased his masterful composing skills . He introduced a whole generation of black folks and others to "South-Africanized" jazz. which was quite different in what we had heard by Dizzy Gillespie, Art Blakeley or Randy Weston and their interpretations. He was bringing it "Straight with No Chaser".
Some of the highlights of the evening's performances was a composition by Herbie Hancock, Cantaloupe Island. Two other originals jumped out at the crowd which spawn several standing ovations when they ended.. Hale Se Di Li Kanna(the Dowry song) and Bajabula Bonke (the Healing Song).
The influence of Dizzy Gillespie and Freddie Hubbard can be heard, along with McCoy Tyner in the playing of pianist Larry Willis, and he shows his debt to John Coltrane as an inspiration on "Mixolydia" as well as his affinity for Brazilian music on "Mas Que Nada." But the core sound was what Masekela called "township bop" — his short trumpet bursts, sometimes seemingly approaching micro-tonal territory, are engrossing celebrations of the melodies of his repertory, which is mostly of South African origin. The buzz after the concert was so loud and the cultural wave became a Tsunami of positive vibes for brother Hugh as he was affectionately called after that night.
By the fall ,I was still hearing rumblings about that summer concert.. only to find out that the very same group was scheduled to perform at our school sometime before the Christmas break. Man! what a blessing! Twice in less than two months. By the time they appeared at our school, most of us was sporting Naturals and wearing sandals, some even wore traditional Dashiki garb and begun learning more about the continent of Africa, particularly, South Africa. I became a life long Hugh Fan, even as he became more and more commercial in his albums, he always brought it back home with a solid menu of fan favorites like Bajabula Bonke, and Cantaloupe Island at the Live concerts.
I will always believe to my dying breath, he believed he was put here to bring joy from the motherland and shine a light on freedom and respect for every one. His Nelson Mandela anthem (Bring him back Home) was globally huge and played a strong role in keeping the fire to the feet of the world powers. I know I will miss him and his musical spirit, but the whole world will miss his humanity. Rest in Heavenly Peace Brother Hugh!
Posted on January 27, 2018 Categories happenings, Jazz Hall of Fame, trendingTags Baby Boomer, Big Black(Dan Rey), classic jazz, classic R&B, classic soul, composers, Dizzy Gillespie, entertainment, festivals, Freddie Hubbard, hard Bop, Henry Franklin, Herbie Hancock, Hugh Masakela, Jazz Crusaders, John Coltrane, Larry Willis, National R&B Society, New York, Old School music, performance, producers, Robert J. Carmack, spiritual jazz, Stix hooper, straight ahead, travel, world music4 Comments on SOUTH AFRICAN MUSICIAN HUGH MASAKELA JOINS THE ANCESTORS-RIP 1939-2018


The fattening room is an ancient practice in calabar which gradually going extinct . The fattening room is a place young women are prepared for womanhood. In ancient times, fat was viewed as a sign of prosperity, fertility and beauty. Young girls are usually taken to the fattening room during puberty. Acceptance into the fattening room was viewed as a privilege as it was a demonstration of virtue, sexual purity and Proved virginity. The ability of the young girl to gain weight in the fattening room was a sign that she possessed all the above mentioned qualities.
A young girl due to enter the fattening room is usually mandated by her father to do so as the girls chastity is viewed as her father's responsibility. The father of the girl also invest by paying what is called "Eme" (coral beads) to appease "Nku" (the river goddess of the house) before she is accepted into the fattening room.The concept is to show that the parents of the girl are wealthy enough to give their daughter a good life.
Once in the Fattening room the girls are kept away from their family members and friends, the only visitors allowed are the elderly women in the community who come to pass on lessons on marital etiquette and acceptable social customs and behavior. The girls are also handfed Heavy meals rich in carbohydrate and fat, Sometimes the girls do not find this process pleasant as they have to consume the food regardless of their appetite. They are also given all-round beauty treatment from head to feet, using what is called ndom (native chalk) and other massage oils made from natural plants. The training and beauty therapy is carried out over a period of one month or more while the girls are each housed in seclusion away from the public as they undergo preparation for marriage and womanhood.Before the end of their stay in the fattening room the girls are circumcised by their mothers this is to ensure she has limited sexual activity and remains chaste till marriage, it also helps her remain faithful in her marriage.
At the end of the Nkugho the girl is ceremoniously revealed to the community to show how big and beautiful she is now , well wishers and potential suitors are also invited to watch her dance.The Ibibio people of Akwa Ibom State also practice the fattening room tradition. The tradition is somewhat similar to that of the Efiks, but the Ibibios call the fattening room "Mbopo".


text:
View cart "Broken DVD 2017 (Original)" has been added to your cart.
John Berger or The Art of Looking (2016)
Art, politics and motorcycles – on the occasion of his 90th birthday John Berger or the Art of Looking is an intimate portrait of the writer and art critic whose ground-breaking work on seeing has shaped our understanding of the concept for over five decades. The film explores how paintings become narratives and stories turn into images, and rarely does anybody demonstrate this as poignantly as Berger.
THE HAPPY PRINCE tells the story of the last days of Oscar Wilde. This great man of letters, once one of the most famous authors in England, is now a superstar on the skids. As Oscar lies on his death bed, the past comes flooding back to him, transporting him to other times and places.
SS-GB
Adapted from Len Deighton's 1978 alternate history novel. A British detective investigates a murder in German-occupied Britain during World War II.
[EX-RENTAL] The Lady In The Van DVD (Original)
The true story of the relationship between Alan Bennett and the singular Miss Shepherd, a woman of uncertain origins who 'temporarily' parked her van in Bennett's London driveway and proceeded to live there for 15 years.
[EX-RENTAL] My Week With Marilyn DVD 2011 (Original)
Sir Laurence Olivier is making a movie in London. Young Colin Clark, an eager film student, wants to be involved and he navigates himself a job on the set. When film star Marilyn Monroe arrives for the start of shooting, all of London is excited to see the blonde bombshell, while Olivier is struggling to meet her many demands and acting ineptness, and Colin is intrigued by her. Colin's intrigue is met when Marilyn invites him into her inner world where she struggles with her fame, her beauty and her desire to be a great actress.
The Boy With The Topknot DVD 2017 (Original)
Born to traditional Punjabi parents and growing up in Wolverhampton, Sathnam Sanghera moves to London after graduating from Cambridge University. Now in his late 20s he is planning to reveal to his family that he will defy expectations of an arranged marriage – but instead learns a painful family secret.
Trauma Series 1 DVD 2018 (Original)
Awaking from a coma to discover his wife has been killed in a car accident, Ben's world may as well have come to an end. A few weeks later, Ben's out of hospital and, attempting to start a new life, he moves home and is befriended by a beautiful young neighbour Charlotte. His life may be turning around but all is not what it seems and, haunted by visions of his dead wife, Ben starts to lose his grip on reality.
Born Equal DVD 2017 (Original)
Four characters living in one neighborhood in London – all living dramatically different lives, all of them on the edge – see their stories unfold.
Williams Blu-Ray 2017 (Original)
This sports documentary tells the story of the Williams Formula 1 team founded by the legendary Sir Frank Williams
Williams DVD 2017 (Original)
Broken DVD 2017 (Original)
Three suburban English families' lives intertwine with tragic consequences.
Mindhorn DVD 2017 (Original)
A washed up actor who played Mindhorn, a secret agent with a bionic eye, returns to the Isle of Man, the area where his most famous role was set, to help catch a killer.


text:
var React = require('react')
var chroma = require('chroma-js')
var sample = require('lodash').sample
var randomHexColor = require('random-hex-color')

var Identicon = React.createClass({
  displayName: 'Identicon',
  getDefaultProps: function() {
    return {
      size: 120,
      colors: [],
      symmetrical: true
    }
  },

  render: function() {
    var size = this.props.size
    var fill = this.props.fill
    var viewBox = [0, 0, size, size].join(' ')
    var colorBoxSize = size / 6

    var randomColor = this.props.colors.length ? sample(colors) : randomHexColor()
    var baseColors = [randomColor, getComplementaryColor(randomColor)]
    var scale = chroma.scale(baseColors).domain([0, 100, 200, 300, 400])
    var identiconColors = baseColors.concat([scale(100).hex(), scale(200).hex(), scale(300).hex(), scale(400).hex()])

    var paths = []
    for(var i = 0; i <= this.props.size / 2; i += colorBoxSize) {
      for(var j = 0; j <= this.props.size; j += colorBoxSize) {
        var color = sample(identiconColors)
        var mirrorColor = this.props.symmetrical ? color : sample(identiconColors)
        paths.push(React.createElement('path', { d: getBoxPath(i, j, colorBoxSize), fill: color, key: '' + i + j }))
        paths.push(React.createElement('path', { d: getBoxPath(this.props.size - i - colorBoxSize, j, colorBoxSize), fill: color, key: '' + i + j + 2 }))
      }
    }

    return (
      React.createElement('svg', {
          xmlns: 'http://www.w3.org/svg/2000',
          viewBox: viewBox,
          width: size,
          height: size,
        },
        paths
      )
    )
  }
})

module.exports = Identicon

function getBoxPath(x, y, z) {
  return [
    'M', x, y,
    'L', x, y + z,
    'L', x + z, y + z,
    'L', x + z, y,
    'L', x, y
  ].join(' ')
}

function getComplementaryColor(color) {
  var colorAsHex = color.replace('#', '0x') ^ '0xfffff'
  var complement =  ('000000' + colorAsHex.toString(16)).slice(-6)
  return '#' + complement
}


Beautiful original tray can be used to display candies, fruits, or other small items, or just to decorate your table in your living room or kitchen. You can use wire, plants, recycled newspapers to make this leafy tray. Rolling recycled newspaper tubes to make such a tray is not only fun, but also green.
1. Hold a few wires in one hand and wrap them with another wire to form the base.
2. Weave along to make the tray.
3. Adjust the tubes to make it like the tray a leafy shape.
4. Tie the end to complete the tray.
5. Paint to the colors you like.


Our veterinarians perform routine surgeries as well some specialized orthopedic surgeries, which are performed by Dr. Boardman.
Dr. Boardman routinely performs ACL surgeries, which involve repairing the anterior cruciate ligament in the knee.
All three veterinarians perform routine surgeries Monday through Friday.


text:
Louis-Raymond-Félix Solier, francoski general, * 1888, † 1967.

Glej tudi
 seznam francoskih generalov

Zunanje povezave
 Generals.dk

Rojeni leta 1888
Umrli leta 1967
Francoski generali
Veterani druge svetovne vojne