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Arja, Katriann
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan. Linköping, .
Sjölander, Daniel
Linköpings universitet, Institutionen för fysik, kemi och biologi, Proteinkemi. Linköpings universitet, Tekniska fakulteten. Linköping, .
Åslund, Alma
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska fakulteten. Linköping, .
Prokop, Stefan
Charite, Germany .
Heppner, Frank L.
Konradsson, Peter
Lindgren, Mikael
Norwegian University of Science and Technology, Norway .
Hammarström, Per
Åslund, Andreas
Enhanced Fluorescent Assignment of Protein Aggregates by an Oligothiophene-Porphyrin-Based Amyloid Ligand2013Ingår i: Macromolecular rapid communications, ISSN 1022-1336, E-ISSN 1521-3927, Vol. 34, nr 9, s. 723-730Artikel i tidskrift (Refereegranskat)
Fluorescent probes identifying protein aggregates are of great interest, as deposition of aggregated proteins is associated with many devastating diseases. Here, we report that a fluorescent amyloid ligand composed of two distinct molecular moieties, an amyloidophilic pentameric oligothiophene and a porphyrin, can be utilized for spectral and lifetime imaging assessment of recombinant A 1-42 amyloid fibrils and A deposits in brain tissue sections from a transgenic mouse model with Alzheimers disease pathology. The enhanced spectral range and distinct lifetime diversity of this novel oligothiopheneporphyrin-based ligand allow a more precise assessment of heterogeneous amyloid morphology compared with the corresponding oligothiophene dye.
Aslund, Andreas
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.
Fluorescent oligo and poly-thiophenes and their utilization for recording biological events of diverse origin-when organic chemistry meets biology.2009Ingår i: Journal of chemical biology, ISSN 1864-6166, Vol. 2, nr 4, s. 161-175Artikel i tidskrift (Refereegranskat)
The technique of using luminescent oligo-thiophenes and luminescent conjugated poly-thiophenes to monitor biological processes has gained increased interest from scientists within different research areas, ranging from organic chemistry and photo-physics to biology since its introduction. The technique is generally straightforward and requires only standard equipment, and the result is available within minutes from sample preparation. In this review, the syntheses of oligo and polythiophenes developed over the last decades are discussed. Furthermore, the utilization of these molecular agents for exploring biological events, e.g., DNA hybridization or protein misfolding events, are covered.
Björefors, Fredrik
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik.
Ekeroth, Johan
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
Borgh, Annika
Liedberg, Bo
Lundström, Ingemar
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi.
Electrochemical Analysis of Self-Assembled Monolayers2001Ingår i: Analysdagarna,2001, 2001Konferensbidrag (Refereegranskat)
4. Blomberg, Eva
Claesson, Per M.
Globotriose- and oligo(ethylene glycol)-terminated self-assembled monolayers: Surface forces, wetting, and surfactant adsorption2006Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 22, nr 24, s. 10038-10046Artikel i tidskrift (Refereegranskat)
A set of oligo( ethylene glycol)-terminated and globotriose-terminated self-assembled monolayers (SAMs) has been prepared on gold substrates. Such model surfaces are well defined and have good stability due to the strong binding of thiols and disulfides to the gold substrate. They are thus very suitable for addressing questions related to effects of surface composition on wetting properties, surface interactions, and surfactant adsorption. These issues are addressed in this report. Accurate wetting tension measurements have been performed as a function of temperature using the Wilhelmy plate technique. The results show that the nonpolar character of oligo( ethylene glycol)-terminated SAMs increases slightly but significantly with temperature in the range 20-55 degrees C. On the other hand, globotriose-terminated SAMs are fully wetted by water at room temperature. Surface forces measurements have been performed and demonstrated that the interactions between oligo( ethylene glycol)-terminated SAMs are purely repulsive and similar to those determined between adsorbed surfactant layers with the same terminal headgroup. On the other hand, the interactions between globotriose-terminated SAMs include a short-range attractive force component that is strongly affected by the packing density in the layer. In some cases it is found that the attractive force component increases with contact time. Both these observations are rationalized by an orientation- and conformation-dependent interaction between globotriose headgroups, and it is suggested that hydrogen-bond formation, directly or via bridging water molecules, is the molecular origin of these effects.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
Petoral Jr., Rodrigo M.
Uvdal, Kajsa
A new route to the formation of biomimetic phosphate assemblies on gold: Synthesis and characterization2006Ingår i: Journal of Colloid and Interface Science, ISSN 1095-7103, Vol. 295, nr 1, s. 41-49Artikel i tidskrift (Refereegranskat)
A biomimetic model system based on long-chain alkanethiols tailored with serine, threonine and tyrosine side-chain groups is created as a platform for the study of phosphorylated amino acids. The phosphorylated analogues are synthesized with protective tert-butyl groups that after assembly on thin polycrystalline gold films are removed in an acidic deprotection solution to form the corresponding phosphate self-assembled monolayers (SAMs). The SAMs are thoroughly characterized with null ellipsometry, contact angle goniometry, infrared reflection–absorption spectroscopy and X-ray photoelectron spectroscopy. The assembly and the subsequent deprotection process are optimized with respect to molecular orientation and chain conformation by varying the incubation time and the exposure time to the deprotection solution. The high quality of the generated SAMs suggests that the present assembly/deprotection approach is an attractive alternative when traditional synthetic routes become demanding because of solubility problems.
Buskas, T
Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden Linkoping Univ, Dept Chem, S-58183 Linkoping, Sweden.
Synthesis of oligosaccharides designed to form micelles, corresponding to structures found in ovarian cyst fluid2000Ingår i: Journal of carbohydrate chemistry, ISSN 0732-8303, E-ISSN 1532-2327, Vol. 19, nr 1, s. 25-51Artikel i tidskrift (Refereegranskat)
The syntheses of alpha-D-GlcpNAc-(1-->4)-beta-D-Galp-(1-->4)-beta-D-GlcNAc-(1-->O)-(CH2)(15)CH3 (1) and fragments thereof, corresponding to structures found in human ovarian cyst fluid, are described. Silver triflate promoted coupling of 3.4,6-tri-O-acetyl-2-azido-2-deoxy-beta-D-glucopyranosyl bromide (12) and galactose acceptor (11) gave a disaccharide donor (13), which was readily transformed into the corresponding bromo-derivative 18. For the synthesis of disaccharide beta-D-Galp-(1-->4)-D-GlcNAc, several differently protected glucosamine accepters were prepared. It was found that cetyl alcohol needed to be introduced after the formation of the P-galactoside bond. Glycosylation of pent-4-enyl 3,6-di-O-benzyl-2-deoxy-2-tetrachlorophthalimido-beta-D-glucopyranoside (30) with (3,4,6-tri-O-acetyl-2-azido-2-deoxy-alpha-D-glucopyranosyl)-(1-->4)-2,3,6-tri-O-benzoyl-alpha-D-galactopyranosyl bromide (18) by use of silver triflate as promoter gave the desired trisaccharide 31. Finally 31 was transformed via coupling to the long alkyl chain aglycon and deprotection into the title compound 1.
Buskas, T.
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
Oscarson, S.
Efficient synthesis of polylactosamine structures through regioselective glycosylations2001Ingår i: Journal of carbohydrate chemistry, ISSN 0732-8303, E-ISSN 1532-2327, Vol. 20, nr 7-8, s. 569-583Artikel i tidskrift (Refereegranskat)
Di-, tri-and tetramers of ß-(1?3)-linked N-acetyllactosamine residues have been synthesised as their methyl glycosides, to be used in ITC binding studies to various galectins. The synthetic strategy involves two types of regioselective glycosylations: couplings of a galactosyl donor to 3,4-diol N-tetrachlorophthalimido glucose acceptors to give the lactosamine monomer building blocks, and subsequent formation of the oligomers through consecutive couplings of lactosamine donors to 2',3',4'-lactosamine acceptors, with high selectivity for the desired products.
Soderberg, E.
Söderberg, E., Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
Fraser-Reid, B.
NPG Research Institute, 710 West Main Street, Durham, NC 27701, United States.
Use of n-pentenyl glycosides as precursors to various spacer functionalities2000Ingår i: Journal of Organic Chemistry, ISSN 0022-3263, E-ISSN 1520-6904, Vol. 65, nr 4, s. 958-963Artikel i tidskrift (Refereegranskat)
Pent-4-enyl ß-D-glucopyranoside and its peracetylated and perbenzylated derivatives are shown to be excellent substrates for preparation of a wide variety of spacer functionalities. The spacer derivatives so obtained are promising substrates for preparing agents such as neo-glycoconjugates, micelies, and liquid crystalline phases, which are of interest for studying various biological and physiological phenomena of carbohydrates.
Campos Melo, Raúl Ivan
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
Wu, Xiongyu
Elgland, Mathias
Novel Trans-Stilbene-based Fluorophores as Probes for Spectral Discrimination of Native and Protofibrillar Transthyretin2016Ingår i: ACS Chemical Neuroscience, ISSN 1948-7193, E-ISSN 1948-7193, Vol. 7, nr 7, s. 924-940Artikel i tidskrift (Refereegranskat)
Accumulation of misfolded transthyretin (TTR) as amyloid fibrils causes various human disorders. Native transthyretin is a neurotrophic protein and is a putative extracellular molecular chaperone. Several fluorophores have been shown in vitro to bind selectively to native TTR. Other compounds, such as thioflavin T, bind TTR amyloid fibrils. The probe 1-anilinonaphthalene-8-sulfonate (ANS) binds to both native and fibrillar TTR, becoming highly fluorescent, but with indistinguishable emission spectra for native and fibrillar TTR. Herein we report our efforts to develop a fluorescent small molecule capable of binding both native and misfolded protofibrillar TTR, providing distinguishable emission spectra. We used microwave synthesis for efficient production of a small library of trans-stilbenes and fluorescence spectral screening of their binding properties. We synthesized and tested 22 trans-stilbenes displaying a variety of functional groups. We successfully developed two naphthyl-based trans-stilbenes probes that detect both TTR states at physiological concentrations. The compounds bound with nanomolar to micromolar affinities and displayed distinct emission maxima upon binding native or misfolded protofibrillar TTR (>100 nm difference). The probes were mainly responsive to environment polarity providing evidence for the divergent hydrophobic structure of the binding sites of these protein conformational states. Furthermore, we were able to successfully use one of these probes to quantify the relative amounts of native and protofibrillar TTR in a dynamic equilibrium. In conclusion, we identified two trans-stilbene-based fluorescent probes, (E)-4-(2-(naphthalen-1-yl)vinyl)benzene-1,2-diol (11) and (E)-4-(2-(naphthalen-2-yl)vinyl)benzene-1,2-diol (14), that bind native and protofibrillar TTR, providing a wide difference in emission maxima allowing conformational discrimination by fluorescence spectroscopy. We expect these novel molecules to serve as important chemical biology research tools in studies of TTR folding and misfolding.
Ederth, Thomas
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
Ekblad, Tobias
Pettitt, Michala E
University of Birmingham.
Conlan, Sheelagh L
Newcastle University.
Du, Chun-Xia
Callow, Maureen E
Callow, James A
Mutton, Robert
Clare, Anthony S
D`Souza, Fraddry
TNO Science and Industry.
Donnelly, Glen
Bruin, Anouk
Willemsen, Peter R
Su, Xueju J
University of Dundee.
Wang, Su
Zhao, Qi
Hederos, Markus
Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms2011Ingår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 3, nr 10, s. 3890-3901Artikel i tidskrift (Refereegranskat)
Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.
Linköpings universitet, Institutionen för fysik, kemi och biologi.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad Fysik. Linköpings universitet, Tekniska högskolan.
Electrochemical Evaluation of the Interfacial Capacitance upon Phosphorylation of Amino Acid Analogue Molecular Films2001Ingår i: Analytical Chemistry, ISSN 0003-2700, Vol. 73, nr 18, s. 4463-4468Artikel i tidskrift (Refereegranskat)
An approach based on electrochemistry to differentiate between phosphorylated and nonphosphorylated amino acid analogues adsorbed on gold is presented. Analogues of serine, threonine, and tyrosine, containing thiohexadecyl headgroups, were synthesized and assembled on gold, and the surface capacitance was evaluated using electrochemical impedance spectroscopy. A procedure for deprotection of tert-butyl phosphate protecting groups, on the monolayer, is also described. Characterizations of the assembled analogues by cyclic voltammetry, infrared spectroscopy, and ellipsometry are used to confirm the insulating properties of the monolayers and the outcome of surface modifications. The results from cyclic voltammetry show good insulating properties for the monolayers even after phosphate deprotection. The infrared measurements reveal well-ordered monolayers, and the thickness from ellipsometry is in good agreement with expectations from molecular modeling. The impedance experiments show a capacitance increase up to 0.6 μF/cm2 as phosphate groups are introduced. The results in this study indicate the possibility of using a surface chemical and impedance spectroscopy approach to detect the kinase/phosphatase activity and kinetics involved in phosphorylation reactions.
Synthesis and Monolayer Characterization of Phosphorylated Amino Acid Analogs2002Ingår i: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 254, nr 2, s. 322-330Artikel i tidskrift (Refereegranskat)
The synthesis of a series of thiols containing phosphorylated and non-phosphorylated serine, threonine, and tyrosine amino acid residues is described. The synthesized molecules, based on 3-mercaptopropionic acid, were assembled onto gold and subsequently characterized using infrared reflection-absorption spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and contact angle goniometry. The ellipsometric analysis indicates that they form densely packed and well-oriented monolayers on gold, with thicknesses that are in good agreement with estimated values from space-filling models. The bulky and space-demanding phosphorylated threonine analog was, however, found to be an exception. The increase in layer thickness when adding a phosphate group to the threonine is only 35% of that observed for the two other analogs. A detailed infrared examination of the influence of cation coordination to the phosphorylated serine analog using calcium and magnesium reveals structural similarities to those of the inorganic phosphate compound calcium hydroxy apatite. We furthermore discuss the application of these monolayers as soft templates for biomineralization.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska högskolan.
Monitoring the interfacial capacitance at self-assembled phosphate monolayers on gold electrodes upon interaction with calcium and magnesium2002Ingår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 74, nr 9, s. 1979-1985Artikel i tidskrift (Refereegranskat)
Electrochemical impedance spectroscopy has been used to evaluate the change in interracial capacitance upon calcium and magnesium coordination to a phosphate-modified electrode. The phosphate electrode was prepared via immobilization of phosphorylated, thiol-containing, serine analogues onto gold. Upon subjection to calcium and magnesium, a substantial drop in capacitance was observed. Magnesium displayed the largest influence on the capacitance: a 27% capacitance drop was observed upon introduction of a 1 mM solution of magnesium ions. The lowered capacitance is a result of a change in the potential and charge distribution at the film/electrolyte interface as the cations coordinate to the phosphate groups. Moreover, the relationship between electrode potential and capacitance has been investigated and reveals a significant difference between monovalent and divalent cations. As complementary information, infrared reflection absorption spectra of the phosphorylated monolayer having different counterions are presented. The results reported in this paper indicate that the phosphorylated amino acid analogue monolayers could be used in investigations of the biochemically important coordination of calcium and magnesium to phosphates and phosphorylated amino acids.
Hook, F.
Höök, F., Department of Applied Physics, Chalmers University of Technology, Göteborg University, SE-412 96 Göteborg, Sweden.
Bivalent-ion-mediated vesicle adsorption and controlled supported phospholipid bilayer formation on molecular phosphate and sulfate layers on gold2002Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 18, nr 21, s. 7923-7929Artikel i tidskrift (Refereegranskat)
Strategies to form supported lipid assemblies on organophosphate- and organosulfate-monolayer-modified gold surfaces are described. By varying surface treatment and the Mg2+ (Ca2+) content in a solution containing phosphatidylcholine vesicles, we demonstrate (i) efficient formation of supported phosphatidylcholine bilayers (SPBs), (ii) formation of supported nonruptured phosphatidylcholine vesicles, and (iii) reduced phosphatidylcholine vesicle adsorption. Thus, by simply varying the solution conditions, the system can be tuned to controlled formation of either a SPB, supported nonruptured vesicles, or a surface with fairly low coverage of nonruptured vesicles. The profound effects induced on the system by Mg2+ and Ca2+ are assigned to a combination of ion-coordination to the surface, ion-association to the lipid headgroups, and osmotic pressure.
Svedhem, S
Linkoping Univ, Dept Chem, SE-58183 Linkoping, Sweden Chalmers Univ Technol, Dept Phys Chem, Gothenburg, Sweden.
Hook, F
Formation and functionalization of surface-supported lipid structures: Building artificial cell membranes on a gold surface.2002Ingår i: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 224, s. 178-COLL-Konferensbidrag (Övrigt vetenskapligt)
Nordeman, P.
Fyrner, Timmy
Antoni, G.
beta-Configured clickable [F-18] FDGs as novel F-18-fluoroglycosylation tools for PET2017Ingår i: New Journal of Chemistry, ISSN 1144-0546, E-ISSN 1369-9261, Vol. 41, nr 18, s. 10231-10236Artikel i tidskrift (Refereegranskat)
In oncology and neurology the F-18-radiolabeled glucose analogue 2-deoxy-2-[F-18]fluoro-D-glucose ([F-18]FDG) is by far the most commonly employed metabolic imaging agent for positron emission tomography (PET). Herein, we report a novel synthetic route to beta-configured mannopyranoside precursors and a chemoselective F-18-fluoroglycosylation method that employ two b-configured [F-18]FDG derivatives equipped with either a terminal azide or alkyne aglycon respectively, for use as a CuAAC clickable tool set for PET. The b-configured precursors provided the corresponding [F-18]FDGs in a radiochemical yield of 77-88%. Further, the clickability of these [F-18]FDGs was investigated by click coupling to the suitably functionalized Fmoc-protected amino acids, Fmoc-N-(propargyl)-glycine and Fmoc-3-azido-L-alanine, which provided the F-18-fluoroglycosylated amino acid conjugates in radiochemical yields of 75-83%. The F-18-fluoroglycosylated amino acids presented herein constitute a new and interesting class of metabolic PET radiotracers.
Ericsson, Emma
Bui, Lan
Enander, Karin
Oriented Protein Immobilization by Chelate Associated PhotochemistryManuskript (preprint) (Övrigt vetenskapligt)
We demonstrate herein the synthesis, characterization and application of a novel chelateassociated photochemistry (CAP) for oriented and robust attachment of biomolecular ligandsto sensing surfaces. The chelation agent is nitrilotriacetic acid (NTA) which is capable ofcoordinating two histidine (His) molecules in the presence of Nickel. Therefore a ligandmodified with a His-sequence can be attached to NTA to form an oriented assembly ofligands on the sensor surface. The ligand is then covalently bound to the surface via a nearbyphotolabile benzophenone (BP) which attacks C-H bonds upon UV light activation. Theligand is then available for analyte interaction. The synthesized compounds used in this studyare based on the well-known organosulphur surface chemistry for proper attachment to goldsurfaces. Besides the two BP and NTA alkane thiols/disulphides we also synthesized a fillermolecule with an oligo (ethylene glycol) (OEG) tail to fine tune the surface composition andto reduce non-specific binding. Results from surface plasmon resonance (SPR) measurementsusing a Biacore 3000 instrument indicate that up to 55% larger analyte response is obtainedwith CAP as compared to the response obtained with the random orientation achieved byphotoimmobilization alone.
Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52074 Aachen, Germany.
Controlled Orientation and Covalent Attachment of Proteins on Biosensor Surfaces by Chelation Assisted Photoimmobilization2013Manuskript (preprint) (Övrigt vetenskapligt)
This report presents a novel method for uniform orientation and covalent attachment of proteins to sensing surfaces, termed Chelation Assisted Photoimmobilization (CAP). Alkanethiols terminated with either nitrilotriacetic acid (NTA), benzophenone (BP) or oligo(ethylene glycol) were synthesized and mixed self-assembled monolayers (SAMs) were prepared on gold and thoroughly characterized by infrared reflection absorption spectroscopy (IRAS), ellipsometry and contact angle goniometry. In the process of CAP, NTA chelates Ni2+ and the complex coordinates a His-tagged ligand in an oriented assembly. The ligand is then photoimmobilized via BP, which forms covalent bonds upon UV light activation. The CAP concept was demonstrated using human IgG-Fc modified with C-terminal hexahistidine tags (His-IgGFc) as the ligand and protein A as the analyte.
In the development of affinity biosensors, uniform orientation of ligand molecules where all analyte binding sites are accessible is often preferred to random orientation. In order to monitor the effect of ligand orientation on analyte response, the ligand-analyte interaction was quantified by surface plasmon resonance analysis, both in the case of CAP and when the ligand was attached by conventional amine coupling on surfaces presenting NTA. Responses were adjusted for differences in ligand immobilization level using IRAS. The normalized analyte response with randomly oriented ligand was 2.5 times higher than that with ligand immobilized by CAP, probably due to molecular crowding effects on the surface and the fact that His-IgGFc is bivalent for protein A. This is a reminder that many other factors than orientation alone may play a decisive role in analyte binding on biosensor surfaces.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
Site-Specific and Covalent Attachment of His-Tagged Proteins by Chelation Assisted Photoimmobilization: A Strategy for Microarraying of Protein Ligands2013Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 29, nr 37, s. 11687-11694Artikel i tidskrift (Refereegranskat)
A novel strategy for site-specific and covalent attachment of proteins has been developed, intended for robust and controllable immobilization of histidine (His)-tagged ligands in protein microarrays. The method is termed chelation assisted photoimmobilization (CAP) and was demonstrated using human IgG-Fc modified with C-terminal hexahistidines (His-IgGFc) as the ligand and protein A as the analyte. Alkanethiols terminated with either nitrilotriacetic acid (NTA), benzophenone (BP); or oligo(ethylene glycol) were synthesized and mixed self-assembled monolayers (SAMs) were prepared on gold and thoroughly characterized by infrared reflection absorption spectroscopy (IRAS), ellipsometry, and contact angle goniometry. In the process of CAP, NTA chelates Ni2+ and the complex coordinates the His-tagged ligand in an oriented assembly. The ligand is then photoimmobilized via BP, which forms covalent bonds upon UV light activation. In the development of affinity biosensors and protein microarrays, site-specific attachment of ligands in a fashion where analyte binding sites are available is often preferred to random coupling. Analyte binding performance of ligands immobilized either by CAP or by standard amine coupling was characterized by surface plasmon resonance in combination with IRAS. The relative analyte response with randomly coupled ligand was 2.5 times higher than when site-specific attachment was used. This is a reminder that also when immobilizing ligands via residues far from the binding site, there are many other factors influencing availability and activity. Still, CAP provides a valuable expansion of protein immobilization techniques since it offers attractive microarraying possibilities amenable to applications within proteomics.
Ericsson, Emma M
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska högskolan.
Controlled orientation and covalent attachment of proteins on biosensor surfaces by Chelation Assisted Photoimmobilization2013Konferensbidrag (Övrigt vetenskapligt)
In the context of surface chemistry for affinity biosensor chips, it is widely accepted that uniform orientation of the immobilized recognition element (ligand) is preferred over random orientation. However, this assumption has often been based on studies where differences in ligand immobilization level have not been taken into account. In this contribution, we present a novel two-step method for homogenous orientation and covalent attachment of proteins to sensing surfaces, called Chelation Assisted Photoimmobilization (CAP). Careful quantification of the effect of ligand orientation on analyte responses was performed by comparing this strategy to immobilization by conventional amine coupling.
In CAP, the chelation agent is nitrilotriacetic acid (NTA) which chelates Ni2+. A His-tagged ligand forms an oriented assembly when binding Ni2+-NTA and is then covalently bound to the surface via photolabile benzophenone (BP), which attacks C-H bonds upon UV light activation. We relied on a surface chemistry based on self-assembled monolayers (SAMs) of oligo(ethylene glycol) (OEG)-containing alkanethiolates on gold. Alkanethiols terminated with either NTA, BP or OEG were synthesized and mixed SAMs were characterized by infrared reflection absorption spectroscopy (IRAS), ellipsometry and contact angle goniometry. IRAS was also used to quantify ligand immobilization levels obtained either by CAP or by amine coupling via the carboxyl groups of an NTA-presenting surface. The model ligand was human IgG-Fc modified with a C-terminal 6xHis-tag and the analyte was Protein A. The ligand-analyte interaction was quantified by a surface plasmon resonance biosensor.
Analyte responses were normalized with respect to the ligand amounts obtained by the two immobilization strategies. Interestingly, the normalized analyte response with randomly oriented ligand was >2 times higher than that with ligand immobilized by CAP. This shows that oriented ligand immobilization is not necessarily a means of increasing the sensitivity of a biosensor. Factors that may influence performance include the valency of the ligand and constraints related to the surface chemistry used for orientation.
Aili, Daniel
Nanyang Technology University, Singapore .
Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten. Linköping, .
Synthesis of oligo(lactose)-based thiols and their self-assembly onto gold surfaces2013Ingår i: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 105, s. 187-193Artikel i tidskrift (Refereegranskat)
The ability to produce monomolecular coatings with well-defined structural and functional properties is of key importance in biosensing, drug delivery, and many recently developed applications of nanotechnology. Organic chemistry has proven to be a powerful tool to achieve this in many research areas. Herein, we present the synthesis of three oligo(lactosides) glycosylated in a (1 → 3) manner, and which are further functionalized with amide-linked short alkanethiol spacers. The oligosaccharides (di-, tetra-, and hexasaccharide) originate from the inexpensive and readily available lactose disaccharide. These thiolated derivatives were immobilized onto gold surfaces, and the thus formed self-assembled monolayers (SAMs) on planar gold were characterized by wettability, ellipsometry and infrared reflection–absorption spectroscopy. Further, the ability of these SAMs to stabilize gold nanoparticles in saline solutions was also demonstrated, indicating that the oligosaccharides may be used as stabilizing agents in gold nanoparticle-based assays.
Modified surfaces using tailor-made oligosaccharides having orthogonal attachments2011Ingår i: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 241, s. 107-CARB-Artikel i tidskrift (Övrigt vetenskapligt)
The ability of fabricating surfaces with low non-specific protein adhesion is of great interest in material science. Studies have shown that self-assembled monolayers (SAMs) covered with carbohydrates moieties have protein resistant characteristics and even marine anti-fouling properties.[1,2]The presented results describe the synthesis of oligosaccharides originating from lactose and mannose. We have also set out to investigate if these orthogonally protected oligosaccharides can be used as spacer molecules and their performance in protein adsorption studies. Using the selected divergence enables incorporation into various surface systems e.g. gold nanoparticles, vesicles or as a conjugate between different macromolecules.
[1] Hederos, M.; Konradsson, P.; Liedberg, B.; Langmuir, 2005, 21, 2971-2980.[2] Fyrner, T.; Konradsson, P.; et. al.; Submitted, 2010.
Lee, Hung-Hsun
Mangone, Alberto
University of Birmingham, UK.
Newcastle University, UK.
Newcastle Universitym, UK.
Saccharide-Functionalized Alkanethiols for Fouling-Resistant Self-Assembled Monolayers: Synthesis, Monolayer Properties, and Antifouling Behavior2011Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 27, nr 24, s. 15034-15047Artikel i tidskrift (Refereegranskat)
We describe the synthesis of a series of mono-, di-, and trisaccharide-functionalized alkanethiols as well as the formation of fouling-resistant self-assembled monolayers (SAMs) from these. The SAls,,Is were characterized using ellipsometry, wetting measurements, and infrared reflection absorption spectroscopy (WAS). We show that the structure of the carbohydrate moiety affects the packing density and that this also alters the alkane chain organization. Upon increasing the size of the sugar moieties (from mono- to di- and trisaccharides), the structural qualities of the monolayers deteriorated with increasing disorder, and for the trisaccharide, slow reorganization dynamics in response to changes in the environmental polarity were observed. The antifouling properties of these SAMs were investigated through protein adsorption experiments from buffer solutions as well as settlement (attachment) tests using two common marine fouling species, zoospores of the green macroalga Ulva linza and cypris larvae of the barnacle Balanus amphitrite. The SAMs showed overall good resistance to fouling by both the proteins and the tested marine organisms. To improve the packing density of the SAMs with bulky headgroups, we employed mixed SAMs where the saccharide-thiols are diluted with a filler molecule having a small 2-hydroxyethyl headgroup. This method also provides a means by which the steric availability of sugar moieties can be varied, which is of interest for specific interaction studies with surface-bound sugars. The results of the surface dilution study and the low nonspecific adsorption onto the SAMs both indicate the feasibility of this approach.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska fakulteten.
Magnusson, Karin
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biokemi. Linköpings universitet, Tekniska högskolan.
Derivatization of a bioorthogonal protected trisaccharide linker: towards multimodal tools for chemical biology2012Ingår i: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 23, nr 6, s. 1333-1340Artikel i tidskrift (Refereegranskat)
When cross-linking biomolecules to surfaces or to other biomolecules, the use of appropriate spacer molecules is of great importance. Mimicking the naturally occurring spacer molecules will give further insight into their role and function, possibly unveil important issues regarding the importance of the specificity of carbohydrate-based anchor moieties, in e.g., glycoproteins and glycosylphosphatidylinositols. Herein, we present the synthesis of a lactoside-based trisaccharide, potentially suitable as a heterobifunctional bioorthogonal linker molecule whereon valuable chemical handles have been conjugated. An amino-derivative having thiol functionality shows promise as novel SPR-surfaces. Furthermore, the trisaccharide has been conjugated to a cholesterol moiety in combination with a fluorophore which successfully assemble on the cell surface in lipid microdomains, possibly lipid-rafts. Finally, a CuI-catalyzed azide-alkyne cycloaddition reaction (CuAAC) confirms the potential use of oligosaccharides as bioorthogonal linkers in chemical biology.
Svensson, Stefan C.T.
Synthesis of tri-, penta-, and hepta-saccharides, functionalized with Orthogonally N-Protected Amino residues at the reducing and non-reducing ends2012Ingår i: Tetrahedron, ISSN 0040-4020, E-ISSN 1464-5416, Vol. 68, nr 33, s. 6712-6720Artikel i tidskrift (Refereegranskat)
The synthesis of four bifunctionalized orthogonally N-protected oligosaccharides derived from lactose and mannose, intended as cross-linking derivatives, is described. The amino sugar at the non-reducing end is derivatized with an N-Boc-protected glycine moiety, and further connected to either a mannose (1→6) disaccharide or (1→3) lactose units (one, two or three) resulting in tri-, penta-, or heptasaccharides. All of the synthesized oligosaccharides have an Nbenzyloxycarbonyl-aminoethyl residue at the reducing end. The fully orthogonal N-Boc/N-Cbz protection group pattern enables further conjugation/derivatization and results in a hydrophilic cross-linking molecule. It was found that the order of the final synthetic steps were crucial to avoid acyl migration. A suitable amide coupling protocol has been applied to introduce the NBoc-protected glycine moiety in alcoholic solvent. The synthesized oligosaccharides will provide a model system to investigate the influence of length, structure and flexibility. The function of the cross-linked substituents thereby provide valuable insights into the role as a spacer molecule.
Gabrielsson, Roger H
Milczarewic, Grzegorz
Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Poland.
Nagarajuc, D. H
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
Inganäs, Olle
Hydrogels of polypyrrole and self doped PEDOT for porous electrodes and supercapacitors2012Manuskript (preprint) (Övrigt vetenskapligt)
The aim of this work is to extend the knowledge of the mechanism of electropolymerization of pyrrole and PEDOT-S by means of in situ electrochemical quartz microbalance with dissipation studies (EQCM-D), which allow us to evaluate the chemical and physical processes during electrochemical deposition of these conductive polymer composites. Meanwhile, the relationship between the morphology of the films and the mechanism of the electropolymerization of pyrrole in presence of PEDOT-S will be discussed. The resulting material is electroactive, black and conducting. This material is a polymer composite where doped polypyrrole chains are found in an environment of doped PEDOT-S chains. They can be identified through the cyclic voltammetry studies of the composite, through element composition and through their optical signatures in electrochromism. The composite has properties suitable for a supercapacitor electrode, and capacitance of up to 650 F/g has been obtained.
Garegg, P.J.
Arrhenius Laboratory, Department of Organic Chemistry, Stockholm University, S-106 91 Stockholm, Sweden.
Johansson, K.-J.
Trumpakaj, Z.
Department of Chemistry, University of Gdansk, PL-80-952 Gdansk, Poland.
Transglucosidation of methyl and ethyl D-glucopyranosides by alcoholysis2002Ingår i: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 337, nr 6, s. 517-521Artikel i tidskrift (Refereegranskat)
The transglucosidations of methyl 4-O-methyl-a- and -ß-D-glucopyranoside in ethanolic camphor-10-sulfonic acid, and of ethyl 4-O-methyl-a- and -ß-D-glucopyranoside in methanolic camphor-10-sulfonic acid, have been studied. Samples were removed at intervals and the proportions of the glucosides determined by GC of their acetates. The results show that the anomer with the inverted configuration predominates in the initially formed product (˜59-70%). This indicates that all the studied reactions proceed via the same mechanism, involving exocyclic C-O cleavage and formation of a glucopyranosylium ion, but that the eliminated alcohol exerts some steric hindrance, which favors the approach of the other alcohol from the opposite side. © 2002 Published by Elsevier Science Ltd.
Simon, Rozalyn
Nyström, Sofie
A Fluorescent Pentameric Thiophene Derivative Detects in Vitro-Formed Prefibrillar Protein Aggregates2010Ingår i: BIOCHEMISTRY, ISSN 0006-2960, Vol. 49, nr 32, s. 6838-6845Artikel i tidskrift (Refereegranskat)
Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of their likely role in the mechanism of fibril formation and because of the growing awareness that these species are likely to play a critical role in the pathogenesis of protein deposition diseases. Herein, we explore the use of an anionic oligothiophene derivative, p-FTAA, for detection of prefibrillar protein aggregates during in vitro fibrillation of three different amyloidogenic proteins (insulin, lysozyme, and prion protein). p-FTAA generally detected prefibrillar protein aggregates that could not be detected by thioflavine T fluorescence and in addition showed high fluorescence when bound to mature fibrils. Second, the kinetics of protein aggregation or the formation of amyloid fibrils of insulin was not extensively influenced by the presence of various concentrations of p-FTAA. These results establish the use of p-FTAA as an additional tool for studying the process of protein aggregation.
Efficient routes to ethyl-2-deoxy-2-phthalimido-1-β-D-thio-galactosamine derivatives via epimerization of the corresponding glucosamine compounds2005Ingår i: Journal of carbohydrate chemistry, ISSN 0732-8303, E-ISSN 1532-2327, Vol. 24, nr 3, s. 297-320Artikel i tidskrift (Refereegranskat)
Short synthetic routes to protected ethyl 2-deoxy-2-phthalimido-1-β-D- thio-galactosamine derivatives via epimerization of the corresponding glucosamine compounds are described. Starting from D-glucosamine hydrochloride, the epimerizations were performed by displacement of presynthesized triflates with nitrite anions and by an oxidation/reduction route. The latter method involved Moffatt oxidation to the corresponding 4-ketohexoses and subsequent reduction using sodium borohydride/ tetrabutylammonium borohydride, zinc borohydride, or lithium tri-sec-butyl borohydride in THF. The displacement route was found to be the preferred method for epimerization of 3-O-acyl (benzoyl) derivatives. For glucosamine compounds with 3-O-etheral- (allyl or benzyl) and 6-O-benzyl protecting groups, the oxidation/reduction route was the most convenient procedure to achieve corresponding galactosamine compounds. The produced galactosamine derivatives will be useful building blocks in the synthesis of antifreeze glycoproteins substances and analogues thereof.
Synthesis of the core tetrasaccharide of Trypanosoma cruzi glycoinositolphospholipids: manp(α1→6)-Manp(α1→4)-6-(2-aminoethylphosphonic acid)-GlcNp(α1→6)-myo-Ins-1-PO42005Ingår i: Journal of Organic Chemistry, ISSN 0022-3263, E-ISSN 1520-6904, Vol. 70, nr 18, s. 7196-7207Artikel i tidskrift (Refereegranskat)
Synthesis of the core tetrasaccharide Manp(α1→6)-Manp(α1→4)-6-(2-aminoethylphosphonic acid)-GlcNp(α1→6)-myo-Ins-1-PO4, found in glycoinositolphospholipids of Trypanosoma cruzi parasites, is described. The key building block, 6-O-(2-azido-3-O-benzyl-6-O-((2-benzyloxycarbonylaminoethyl)phosphonic acid benzyl ester)-2-deoxy-α-d-glucopyranosyl)-1-di-O-benzylphosphoryl-4,5-O-isopropylidene-2,3-O-(d-1,7,7-trimethyl[2,2,1]bicyclohept-6-ylidene)-d-myo-inositol, was synthesized using a partially protected glucosyl d-camphorinositolphosphate and a (2-benzyloxycarbonylaminoethyl)phosphonic acid derivative in a regioselective phosphonate esterfication. Elongation with ethyl 2-O-benzoyl-3,4,6-tri-O-benzyl-α-d-mannopyranosyl-(1→6)-2,3,4-tri-O-benzyl-1-α-d-thiomannopyranoside using dimethyl(methylthio)sulfonium trifluoromethanesulfonate gave a fully protected tetrasaccharide which was successfully deprotected subsequently with sodium methoxide, sodium in liquid ammonia, and aq hydrochloric acid to give title compound.
Synthesis of the Trypanosoma cruzi LPPG heptasaccharyl myo-inositol2006Ingår i: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 128, nr 10, s. 3414-3419Artikel i tidskrift (Refereegranskat)
Synthesis of the heptasaccharyl myo-inositol found in Trypanosoma cruzi lipopeptidophosphoglycan was accomplished using a convergent assembly of three building blocks. The target compound is the first complete 2-aminoethyl phosphonic acid substituted glycan related to the glycosylphosphatidylinositol anchor family to be synthesized. The order of assembly enables synthesis of phosphoinositol oligosaccharides related to other glycosylinositolphospholipids in Tr. cruzi, the protozoan parasite causing Chagas' disease, which is endemic in South America.
Mimicking the properties of antifreeze glycoproteins: synthesis and characterization of a model system for ice nucleation and antifreeze studies2005Ingår i: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 109, nr 33, s. 15849-15859Artikel i tidskrift (Refereegranskat)
Synthesis of β-D-Gal-(1 → 3)-β-D-GalNAc coupled to HOC2H4NHCOC15H30SH is described. This compound was coadsorbed at various proportions with C2H5OC2H4NHCOC15H30SH to form statistically mixed self-assembled monolayers (SAMs) on gold in an attempt to mimic the properties of the active domain in antifreeze glycoproteins (AFGPs). The monolayers were characterized by null ellipsometry, contact angle goniometry, X-ray photoelectron spectroscopy, and infrared reflection−absorption spectroscopy. The disaccharide compound adsorbed preferentially, and SAMs prepared at a solution molar ratio >0.3 displayed total wetting. The mixed SAMs showed well-organized alkyl chains up to a disaccharide surface fraction of 0.8. The amount of gauche conformers in the alkyls increased rapidly above this point, and the monolayers became disordered and less densely packed. Furthermore, the generated mixed SAMs were subjected to water vapor at constant relative humidity and the subsequent ice crystallization on a cooled substrate was monitored via an optical microscope. Interestingly, rapid crystallization occurred within a narrow range of temperatures on mixed SAMs with a high disaccharide content, surface fraction >0.3. The reported crystallization temperatures and the ice layer topography were compared with results obtained for a much simpler reference system composed of −OH/−CH3 terminated n-alkanethiols in order to account for changes in topography of the water/ice layer with surface energy. Although preliminary, the obtained results can be useful in the search for the molecular mechanism behind the antifreeze activity of AFGPs.
Synthesis and self-assembly of galactose-terminated alkanethiols and their ability to resist proteins2005Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 21, nr 7, s. 2971-2980Artikel i tidskrift (Refereegranskat)
The synthesis of two galactose-terminated alkanethiols with the structural formula X−OC2H5NHCO(CH2)15SH (X = 2,3,4,6-tetra-O-methyl-β-d-Gal or β-d-Gal) is described. Single-component and mixed self-assembled monolayers (SAMs) of the methylated and nonmethylated compounds were prepared on gold and subsequently characterized with ellipsometry, contact angle goniometry, and infraredreflection−absorption spectroscopy. Studies of the irreversible protein adsorption onto the SAMs using ex-situ ellipsometry revealed very low levels of fibrinogen and lysozyme adsorption onto mixed SAMs displaying advancing water contact angles between 24° and 45° and below 45°, respectively. A monomethylated compound (X = 6-O-methyl-β-d-Gal) was also synthesized and assembled on gold. This particular compound was found to possess wettability properties corresponding to the low adsorption regime of the mixed SAMs, and the results from the same set of fibrinogen and lysozyme adsorption experiments showed very low levels of protein adsorption. Our findings suggest that the protein rejecting properties rely on a fine balance between the surface energy and/or hydrogen bond donating/accepting properties of the SAM surface.
Herland, Anna
Björk, Per
Nilsson, K. Peter R.
Electroactive luminescent self-assembled bio-organic nanowires: Integration of semiconducting oligoelectrolytes within amyloidogenic proteins2005Ingår i: Advanced Materials, ISSN 0935-9648, Vol. 17, nr 12, s. 1466-1471Artikel i tidskrift (Refereegranskat)
Olsson, Johan D. M.
Synthesis of a regioregular zwitterionic conjugated oligoelectrolyte, usable as an optical probe for detection of amyloid fibril formation at acidic pH2005Ingår i: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 127, nr 7, s. 2317-2323Artikel i tidskrift (Refereegranskat)
Changes of the optical properties of conjugated polyelectrolytes have been utilized to monitor noncovalent interactions between biomolecules and the conjugated polyelectrolytes in sensor applications. A regioregular, zwitterionic conjugated oligoelectrolyte was synthesized in order to create a probe with a defined set of optical properties and hereby facilitate interpretation of biomolecule−oligoelectrolyte interactions. The synthesized oligoelectrolyte was used at acidic pH as a novel optical probe to detect amyloid fibril formation of bovine insulin and chicken lysozyme. Interaction of the probe with formed amyloid fibrils results in changes of the geometry and the electronic structure of the oligoelectrolyte chains, which were monitored with absorption and emission spectroscopy.
Arrhenius Laboratory, Dept. Organ. Chem., Stockholm U., Stockholm, Sweden.
Department of Chemistry, University of Gdansk, PL-80-952, Gdansk, Poland.
Transglucosidation of methyl and ethyl D-glucofuranosides by alcoholysis2001Ingår i: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 332, nr 1, s. 33-39Artikel i tidskrift (Refereegranskat)
The acid catalyzed ethanolysis of methyl 5-O-methyl-a- and -ß-D-glucofuranoside and the analogous methanolysis of ethyl 5-O-methyl-a- and -ß-D-glucofuranoside have been investigated. For all four reactions, the primarily formed transglycosylation product was a single glycoside that had the opposite anomeric configuration to the starting material. This strongly indicates that a D-glucose methyl ethyl acetal is first formed and is then ring closed by a nucleophilic attack by HO-4, giving either the starting material or a transglycosylation product with the opposite anomeric configuration. Low percentages of the methyl ethyl acetals and of dimethyl acetals were also observed in the reaction product during the methanolysis reactions. © 2001 Elsevier Science Ltd.
Karlsson, Anna-Carin
Linköpings universitet, Institutionen för fysik, kemi och biologi, Zoologi. Linköpings universitet, Tekniska högskolan.
Jensen, Per
Laur, Katriann
Laska, Matthias
Red junglefowl have individual body odors2010Ingår i: JOURNAL OF EXPERIMENTAL BIOLOGY, ISSN 0022-0949, Vol. 213, nr 10, s. 1619-1624Artikel i tidskrift (Refereegranskat)
Olfaction may play an important role in regulating bird behavior, and has been suggested to be involved in feather-pecking. We investigated possible differences in the body odors of red junglefowl females by using an automated olfactometer which assessed the ability of trained mice to discriminate between the odors of uropygial gland secretions (the main carrier of potential individual odors in chickens) of six feather-pecked and six non-pecked birds. All mice were clearly able to discriminate between all individual red junglefowl odors, showing that each bird has an individual body odor. We analyzed whether it was more difficult to discriminate between the odors of two feather-pecked, or two non-pecked birds, than it was to discriminate between the odors of two randomly selected birds. This was not the case, suggesting that feather-pecked birds did not share a common odor signature. Analyses using gas chromatography and mass spectrometry showed that the composition of aliphatic carboxylic acids in uropygial gland secretions differed consistently between individuals. However, chemical composition did not vary according to feather-pecking status. We conclude that red junglefowl have individual body odors which appear to be largely based on differences in the relative abundance of aliphatic carboxylic acids, but there is no evidence of systematic differences between the body odors of pecked and non-pecked birds.
Karlsson, Roger H.
Hamedi, Mahiar
Iron Catalyzed Polymerization of Alkoxysulfonate-Functionalized EDOT gives2007Ingår i: Chemistry of Materials, ISSN 0897-4756, E-ISSN 1520-5002Artikel i tidskrift (Refereegranskat)
Karlsson, Roger
Wigenius, Jens
Liu, Xianjie
Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska högskolan.
Fahlman, Mats
Iron-Catalyzed Polymerization of Alkoxysulfonate-Functionalized 3,4-Ethylenedioxythiophene Gives Water-Soluble Poly(3,4-ethylenedioxythiophene) of High Conductivity2009Ingår i: Chemistry of Materials, ISSN 0897-4756, E-ISSN 1520-5002, Vol. 21, nr 9, s. 1815-1821Artikel i tidskrift (Refereegranskat)
Chemical polymerization of a 3,4-ethylenedioxythiophene derivative bearing a sulfonate group (EDOTS) is reported. The polymer, PEDOT-S, is fully water-soluble and has been produced by polymerizing EDOT-S in water, using Na2S2O8 and a catalytic amount of FeCl3. Elemental analysis and XPS measurements indicate that PEDOT-S is a material with a substantial degree of self-doping, but also contains free sulfate ions as charge-balancing counterions of the oxidized polymer. Apart from self-doping PEDOT-S, the side chains enable full water solubility of the material; DLS studies show an average cluster size of only 2 nm. Importantly, the solvation properties of the PEDOT-S are reflected in spin-coated films, which show a surface roughness of 1.2 nm and good conductivity (12 S/cm) in ambient conditions. The electro-optical properties of this material are shown with cyclic voltammetry and spectroelectrochemical experiment reveals an electrochromic contrast (similar to 48% at lambda(max) = 606 nm).
40. Lahmann, M
Garegg, PJ
Oscarson, S
Synthesis of a polyphosphorylated GPI-anchor core structure2002Ingår i: Canadian journal of chemistry (Print), ISSN 0008-4042, E-ISSN 1480-3291, Vol. 80, nr 8, s. 1105-1111Artikel i tidskrift (Refereegranskat)
Using a linear assembly approach a highly differentially protected derivative of the common GPI-anchor core structure (alpha-D-Man-(1-->6)-alpha-D-Man-(1-->2)-alpha-D-Man-(1-->4)-alpha-D-GlcNH(2)-(1-->6)-D-myo-inositol) has been synthesized. All mannose donors were prepared from a common thioglycoside precursor (1), and coupled to GlcN(3)-myo-inositol acceptor 5 in a linear five-step glycosylation-deprotection sequence in 49% overall yield, to give the key intermediate 10, with orthogonal temporary protecting groups at the 6", 2", 6', and 2 positions of the trimannoside motif and at the 1 and 2 positions of the inositol part. Consecutive removal of the temporary protecting groups in the trimannoside moiety followed by phosphorylation, gave a tetraphosphosphate derivative in 60% overall yield. Removal of a camphor acetal afforded a 1,2-inositol diol, which was converted to a 1,2-cyclic phosphate using commercial methyl dichlorophosphate (-->17, 95%). One-step deprotection using sodium in liquid ammonia afforded the target polyphosphorylated core structure 18 (60%), which will be tested for metabolic insulin action.
Li, Feng
Wageningen University.
Martens, Aernout A
de Wolf, Frits A
Cohen Stuart, Martien A
Sudholter, Ernst J R
Delft University Technology.
Marcelis, Antonius T M
Leermakers , Frans A M
Formation of nanotapes by co-assembly of triblock peptide copolymers and polythiophenes in aqueous solution2009Ingår i: SOFT MATTER, ISSN 1744-683X , Vol. 5, nr 8, s. 1668-1673Artikel i tidskrift (Refereegranskat)
Nanotapes are formed by the co-assembly of triblock peptide copolymers with an amino acid-substituted polythiophene derivative (PTT). The driving force for the assembly is ionic interaction (complex coacervation). These nanotapes were visualized by atomic force microscopy and confocal laser scanning microscopy. The interactions between the triblock peptide copolymers and the PTT are also expressed in the steady state and time resolved fluorescence spectra. The steady-state spectra indicate that upon interaction with the peptide copolymer, the backbone of the PTT adopts a rather twisted, and definitely less, aggregated conformation. The time-resolved fluorescence decay studies further confirm this interpretation. The structure of these nanotapes at the mesoscopic scale depends, among other physical chemical parameters, on the concentrations of its constituents.
Palaniswamy, Ganesan
de Jong, Menno R.
Biomade Technol Fdn.
Marcelis, Antonius T. M.
Sudholter, Ernst J. R.
Delft University of Technology.
Cohen Stuart, Martien A.
Leermakers, Frans A. M.
Nanowires Formed by the Co-Assembly of a Negatively Charged Low-Molecular Weight Gelator and a Zwitterionic Polythiophene2010Ingår i: ChemPhysChem, ISSN 1439-4235, E-ISSN 1439-7641, Vol. 11, nr 9, s. 1956-1960Artikel i tidskrift (Refereegranskat)
Conjugated organic nanowires have been prepared by co-assembling a carboxylate containing low-molecular weight gelator (LMWG) and an amino acid substituted polythiophene derivative (PTT). Upon introducing the zwitterionic polyelectrolyte PTT to a basic molecular solution of the organogelator, the negative charges on the LMWG are compensated by the positive charges of the PTT. As a result, nanowires form through co-assembly. These nanowires are visualized by both transmission electron microscopy (TEM) and atomic force microscopy (AFM). Depending on the concentration and ratio of the components these nanowires can be micrometers long. These measurements further suggest that the aggregates adopt a helical conformation. The morphology of these nanowires are studied with fluorescent confocal laser scanning microscopy (CLSM). The interactions between LMWG and PTT are characterized by steady-state and time-resolved fluorescence spectroscopy studies. The steady-state spectra indicate that the backbone of the PTT adopts a more planar and more aggregated conformation when interacting with LMWG. The time-resolved fluorescence decay studies confirm this interpretation.
Lindberg, Jan
Efficient synthesis of phospholipids from glycidyl phosphates2002Ingår i: Journal of Organic Chemistry, ISSN 0022-3263, E-ISSN 1520-6904, Vol. 67, nr 1, s. 194-199Artikel i tidskrift (Refereegranskat)
New efficient routes to enantiopure phospholipids, starting from (S)-glycidol, are described. Lysophosphatidic acids and phosphatidic acids were obtained in good overall yields from (S)-glycidol, in only three and four steps, respectively. Moreover, the strategy can also be used to produce phosphatidylcholines in three steps. Using dialkylphosphoramidites, (S)-glycidol was phosphorylated to give (R)-1-O-glycidyl dialkyl phosphates. Regiospecific epoxide opening, using hexadecanol or cesium palmitate, followed by phosphate deprotection, provided lysophosphatidic acids. 2-O-Esterification prior to phosphate deprotection provided 1,2-O-diacyl and 1-O-alkyl-2-O-acyl phosphatidic acids. Phosphorylation of (S)-glycidol using phosphorus oxychloride followed by in situ treatment with choline tosylate produced (R)-glycidyl phosphocholine. Subsequent nucleophilic opening of the epoxide using cesium palmitate produced 1-O palmitoyl-sn-glycero-3-phosphocholine, which has been used in syntheses of phosphatidylcholines.
Ohberg, L.
Öhberg, L., Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.
Efficient routes to glucosamine-myo-inositol derivatives, key building blocks in the synthesis of glycosylphosphatidylinositol anchor substances2002Ingår i: Tetrahedron, ISSN 0040-4020, E-ISSN 1464-5416, Vol. 58, nr 7, s. 1387-1398Artikel i tidskrift (Refereegranskat)
Short synthetic routes to protected derivatives of 2-amino-2-deoxy-a-D-glucopyranosyl-(1?6)-D-myo-inositol are described. Various 2-azido-2-deoxy-glucosyl donors were synthesized, starting from D-glucal or glucosamine hydrochloride. Derivatives of 1,2- and 2,3- D-myo-inositol-camphanylidene acetals were prepared to function as glycosyl acceptors. The subsequent glycosylations produced useful building blocks for the synthesis of GPI-anchor substances. © 2002 Elsevier Science Ltd. All rights reserved.
Svensson, Stefan
Pahlsson, P.
Synthesis of galactoglycerolipids found in the HT29 human colon carcinoma cell line2002Ingår i: Tetrahedron, ISSN 0040-4020, E-ISSN 1464-5416, Vol. 58, nr 25, s. 5109-5117Artikel i tidskrift (Refereegranskat)
Synthesis of three galactoglycerolipids (3-O-(ß-D-galactopyranosyl)-1-O-hexadecyl-2-O-palmitoyl-sn-glycerol, 3-O-(a-D-galactopyranosyl-(1?4)-ß-D-galactopyranosyl)-1-O- hexadecyl-2-O-palmitoyl-sn-glycerol, 3-O-(a-D-galactopyranosyl-(1?4)-ß-D-galactopyranosyl)-1-O- hexadecyl-sn-glycerol), and the corresponding glycerolipid (1-O-hexadecyl-2-O-palmitoyl-sn-glycerol) is described. The first two compounds were recently identified in the human colon carcinoma cell line HT29. The three-carbon synthon (S)-glycidol was used for construction of the glycerol moiety. Glycosylation of (S)-glycidol with protected galactosyl and digalactosyl donors produced galactosyl and digalactosyl glycidols. Lewis acid catalyzed opening of the epoxide produced protected galactosyl and digalactosyl glycerolipids. Deprotection, or palmitoylation followed by deprotection, yielded the target compounds. The corresponding glycerolipid was synthesized analogously and an oxidation-reduction procedure for tritiation was developed. The synthesized compounds will be used in studies of the role of galactosyl glycerolipids in differentiation and colon cancer development. © 2002 Elsevier Science Ltd. All rights reserved.
Malysheva, Lyuba
Bogolyubov Institute for Theoretical Physics, 03680, Kiev, Ukraine.
Onipko, Alexander
Valiokas, Ramūnas
Department of Nanoengineering, Center for Physical Sciences and Engineering, Savanoriu 231, LT-02300 Vilnius, Lithuania.
Spectroscopic Characterization and Modeling of Methyl- and Hydrogen-Terminated Oligo (ethylene glycol) Self-Assembled Monolayers2012Ingår i: The Journal of Physical Chemistry C, ISSN 1932-7447, E-ISSN 1932-7455, Vol. 116, nr 22, s. 12008-12016Artikel i tidskrift (Refereegranskat)
Two series of oligo (ethylene glycol) (OEG) thiol compounds HS-(CH2CH2O)nR with R = CH3, H and n = 5, 6, 7, have been synthesized and used to form self-assembled monolayers (SAMs) on gold. The data from null ellipsometry, infrared reflection-absorption spectroscopy and ab initio calculations of this type of OH- and CH3-terminated OEG SAMs are used to examine the rarely addressed in-SAM orientation of oligo (ethylene glycols) and to provide detailed assignments of infrared bands in the fingerprint and CH-stretching regions. Based on these results, a new spectral band has been observed at 2947 cm-1 and identified by the firstprinciple calculations as localized vibrations that are specific for hydrogen-terminated OEG thiolate SAMs. This band can be used as an indicator of a high crystalline like ordering. It is further more stressed that theory agrees with the experimentally obtained CH-stretching spectra remarkably well if, and only if, the OEG helix axis within studied SAMs is tilted by about 20o with respect to the surface normal.
Ikenberg, Kristian
University Hospital of Zurich.
Fransson, Sophia
Röcken, Christoph
Christian-Albrechts-University, Kiel.
Moch, Holger
Aguzzi, Adriano
Structural typing of systemic amyloidoses by luminescent-conjugated polymer spectroscopy.2010Ingår i: The American journal of pathology, ISSN 1525-2191, Vol. 176, nr 2, s. 563-574Artikel i tidskrift (Refereegranskat)
Most systemic amyloidoses are progressive and lethal, and their therapy depends on the identification of the offending proteins. Here we report that luminescent-conjugated thiophene polymers (LCP) sensitively detect amyloid deposits. The heterodisperse polythiophene acetic acid derivatives, polythiophene acetic acid (PTAA) and trimeric PTAA, emitted yellow-red fluorescence on binding to amyloid deposits, whereas chemically homogeneous pentameric formic thiophene acetic acid emitted green-yellow fluorescence. The geometry of LCPs modulates the spectral composition of the emitted light, thereby reporting ligand-induced steric changes. Accordingly, a screen of PTAA-stained amyloid deposits in histological tissue arrays revealed striking spectral differences between specimens. Blinded cluster assignments of spectral profiles of tissue samples from 108 tissue samples derived from 96 patients identified three nonoverlapping classes, which were found to match AA, AL, and ATTR immunotyping. We conclude that LCP spectroscopy is a sensitive and powerful tool for identifying and characterizing amyloid deposits.
Olsson, Johan
Enantiomeric substituents determine the chirality of luminescent conjugated polythiophenes2004Ingår i: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 37, nr 17, s. 6316-6321Artikel i tidskrift (Refereegranskat)
Chiral isomers of 3-substituted polythiophenes with amino acid functiontionalized side chains are compared. The polymers show pH-dependent absorption, emission, and circular dichroism spectra in buffered aqueous solution. At pH equal to pI of the amino acid, the backbones adopt nonplanar helical conformations, and the polymer chains are separated from each other. Increasing pH leads to more planar conformations of the backbones and an aggregation of the polymer chains occurs. A lower pH will also lead to more planar conformation of the backbones, but aggregation of the polymer chains appears to be absent. The nonplanar to planar transition of the polymer backbone and the separation/aggregation of different polymer chains is not affected by stereochemistry of the zwitterionic side chain. The two isomers have almost identical pH-dependent absorption and emission spectra. However, the chirality of the zwitterionic side chain is reflected in the conformation of the polymer backbone, giving rise to a right-handed and left-handed helical form of polythiophene chains since the induced circular dichroism patterns of the two polymers are mirror images.
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
Stabo-Eeg, Franz
The Norwegian University of Scinece and Technology.
The Norwegian University of Science and Technology.
Chiral Recognition of a Synthetic Peptide Using Enantiomeric Conjugated Polyelectrolytes and Optical Spectroscopy2005Ingår i: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 38, s. 6813-6821Artikel i tidskrift (Refereegranskat)
Berg, Ina
Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biokemi.
Stabo-Eeg, Frantz
Westermark, Gunilla
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för biomedicin och kirurgi, Avdelningen för medicinsk cellbiologi.
Lannfelt, Lars
Nilsson, Lars N G
Imaging distinct conformational states of amyloid-β fibrils in Alzheimer's disease using novel luminescent probes2007Ingår i: ACS Chemical Biology, ISSN 1554-8929, Vol. 2, nr 8, s. 553-560Artikel i tidskrift (Refereegranskat)
Using luminescent conjugated polyelectrolyte probes (LCPs), we demonstrate the possibility to distinguish amyloid-β 1-42 peptide (Aβ1-42) fibril conformations, by analyzing in vitro generated amyloid fibrils of Aβ1-42 formed under quiescent and agitated conditions. LCPs were then shown to resolve such conformational heterogeneity of amyloid deposits in vivo. A diversity of amyloid deposits depending upon morphology and anatomic location was illustrated with LCPs in frozen ex vivo brain sections from a transgenic mouse model (tg-APPswe) of Alzheimer's disease. Comparative LCP fluorescence showed that compact-core plaques of amyloid β precursor protein transgenic mice were composed of rigid dense amyloid. A more abundant form of amyloid plaque displayed morphology of a compact center with a protruding diffuse exterior. Surprisingly, the compact center of these plaques showed disordered conformations of the fibrils, and the exterior was composed of rigid amyloid protruding from the disordered center. This type of plaque appears to grow from more loosely assembled regions toward solidified amyloid tentacles. This work demonstrates how application of LCPs can prove helpful to monitor aggregate structure of in vivo formed amyloid deposits such as architecture, maturity, and origin.


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Contact Us | (877) 318-7273
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Parent Divorce Associated with Academic Struggles in College Students
Home College dropout Parent Divorce Associated with Academic Struggles in College Students
College dropout
Research shows that parent divorce can have an effect on a child's academic performance, regardless of their age. Younger children may have trouble concentrating in class and display "unexplained" emotional outbursts. Teenagers may struggle to complete assignments, especially if their parents have shared custody, homework monitoring is inconsistent, or access to school supplies depends on whose house they are staying at. Although they may live outside the home, college students are not the exception to this. Young adults may continue to experience the effects of their parents' divorce in college through academic struggles and relationship struggles.
How Does Divorce Affect One's Academic Experience?
One study suggests that children of divorced parents are more likely to be held back a grade, receive poor grades, and have fewer postsecondary education goals. Possible explanations may be that children of divorced parents are more likely to experience depression or that the stress of the divorce may affect one's motivation and self-esteem. Perceived parent support may also affect one's decision to pursue a college career, especially if divorced parents have different values around education or one's career path.
Aren't College Students Less Likely to Experience the Effects of Their Parents Divorce?
It's a mistake to think college students are completely separated from parents. They're still very dependent on their parents for economic support, emotional support, advice and guidance. Ideally, students would like to come and go from being dependent to being independent, and gradually have that be under their control. But divorce can take away that sense of control for many young adults.
Young adults who are on the verge of adulthood are under a lot of pressure to fit into the role society has in store for them. Young adulthood is a criticial period of establishing independence, from entering college and finding a job to starting their own family and purchasing a home, that can bring up a lot of self-doubt and pressure to succeed. Because of this, many young adults fail to launch into adulthood.
Learning differences, mental health struggles, and high stress levels often go under the radar when families are managing complex family dynamics. When these issues are misunderstood or unaddressed, young adults may continue to face barriers to independence and struggle with reaching out to their parents as their support system.
Parent Coaching in Transitional Programs for College-Aged Students
Foundations Asheville functions as a supportive community of students, Therapists, Educators, Community Liaisons, and Mentors who collaborate to establish achievable goals for each young adult in the program, all the while working side-by-side toward realizing those goals. In addition to individualized support, parent coaching helps family members get on the same page when it comes to supporting their child's academic and career goals.
Many young adults struggle with communicating their needs and asking for support from their parents, as they've internalized narratives that they are meant to ride solo and take care of themselves. They may be used to picking and choosing the highlights of what they want to share with their parents and hiding other more personal details. While it is expected for communication styles to change between parents and their children in young adulthood, many families become disconnected from each other during this transition.
Transitional programs guide families in setting boundaries and changing their expectations as our professionals work closely with families and their young adults to determine the appropriate level of support they may need. Our parent coaching model is client-centered, that is, conversations revolve around how to empower students to succeed. This involves a degree of resolving family conflict by improving communication styles, but is also focused on how to move forward in rebuilding relationships and trust.
Foundations Asheville Can Help
Foundations Asheville is a program for young adults ages 18-24 who are struggling to find the motivation to launch into adulthood. Many of our students struggle with anxiety, depression, social skills, and learning difficulties. This program is committed to helping young adults develop and sharpen the skills they need to be successful in the real world. There is a focus on teaching students how to enter the workforce, develop vocational trades, and functional living skills. Foundations Asheville gives young adults the opportunity to gain confidence, find their purpose, and learn useful skills that will help them navigate through the adult years.
For more information on how to motivate your struggling young adult, call 18773187273. We can help your family today!
Higher Levels of Stress Among College Dropouts Whose Parents Have Degrees
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Company foundationsasheville Internet Source: Webform Internet Source Description: Blog Program: foundationsasheville Lead Source SEO
Foundations Asheville is a young adult transition community. The program serves young men and young women ages 18-24, who are looking to acquire the tools to make the transition from adolescence to adulthood.

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Deadline: February 1
Applicants must
- Be a graduating senior from or graduate of Guilford High School or a Mendota Township High School;
- Have a GPA of at least 2.0/4.0;
- Have financial need; and
- Be pursuing a vocational career.
Susan Munson Gilmore was born in Mendota, Illinois and graduated from Mendota High School and the University of Illinois. She began her teaching career in 1966 and taught Home Economics at Guilford High School. She had two children, Margaret and Tom, and enriched the lives of those around her.
Taking into consideration the background and criteria of this scholarship, why are you the best candidate to receive it? You may want to discuss your past academic or personal achievements, leadership skills, community and school involvement, or obstacles you've overcome.
Would you like to support the Susan Kay Munson Gilmore Memorial Scholarship? You can make a donation directly to this fund, to help grow its impact and ability to provide educational resources for students in our community — forever!
How to Register & Apply
Scholarships Main Page
Scholarship Eligibility & FAQs
Scholarship Resources
Scholarship Flyer Directory
Counselors & References
Deadlines:
Unless otherwise noted, scholarships have a deadline of February 1 at 5 p.m.
Contact Ciara Stahly
Email Ciara


In addition to nearby shopping and dining options at Park Meadows Mall , our hotel is in the heart of Denver Tech Center and near Comcast Communications and other corporate locations. Our hotel is within 10 miles of Cherry Creek Reservoir Trail for hiking and recreation. Just minutes away you'll find Top Golf, a sprawling entertainment venue with a high-tech driving range and an entertaining lounge with drinks and games.
A fun family atmosphere with family and friends. Golf driving range, games and fun.
Enjoy a relaxing day fishing, boating, nature hike and more.


Giving out green light, this 200mW 532nm Focus Green Beam Light Laser Pointer Pen with 18650 Rechargeable Battery aims at providing you with high-performance light effect. Equipped with high-grade circuit, this laser pointer pen can work regularly. The focus design enables better light effect. So this laser pointer pen is perfect for teaching, guiding and so on. This power-saving laser pointer pen can be used for long lasting time due to its rechargeable battery. For this reason, this laser pointer pen can meet your demands perfectly.


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Home Schools Riverchase's Turney named Distinguished Principal for Alabama District 4
Riverchase's Turney named Distinguished Principal for Alabama District 4
by JON ANDERSON
Photo by Jon Anderson.
Riverchase Elementary School Principal Alice Turney greets kindergartner David Juarez in the hallway before school Dec. 3.
Riverchase Elementary School Principal Alice Turney has been named the 2021 Distinguished Principal for District 4 of the Alabama Association of Elementary School Administrators.
Turney has been principal at Riverchase since 2014, when she moved up from the assistant principal spot to fill the position of retiring Principal Dianne Baggett.
Since that time, Riverchase Elementary was named a Blue Ribbon School of Excellence by the U.S. Department of Education in 2018 and achieved science, technology, engineering and math certification from Cognia, a national accreditation agency, in 2019.
The school also has received more than $20,000 in grant money from the Hoover City Schools Foundation during her tenure.
Turney has served as a principal mentor for the Council for Leaders in Alabama Schools in 2019 and 2021 and this year is the District 4 president of the Alabama Association of Elementary School Administrators. She now is in contention for the statewide Distinguished Principal award.
The Distinguished Principal Award is given to principals who are respected and a force for positive change, contribute to the well-being of the educational community, help create a positive environment, motivate and inspire others, go beyond what is expected, serve the community, show strong leadership, operate an orderly and purposeful school, and set high expectations that lead to increased student achievement.
Turney grew up in Apison, Tennessee, near Chattanooga. She graduated from Ooltewah High School, earned a bachelor's degree in elementary education and master's degree in educational leadership from the University of West Georgia and a doctorate in educational leadership from Argosy University. She is currently pursuing a certificate in school management and leadership from the Harvard Graduate School of Education.
She began her career as a teacher and student support specialist at Eastside Elementary in the Metro Atlanta school system from 1997 to 2002, then was an assistant principal at New Manchester Elementary, also in Metro Atlanta, from 2002 to 2005 before moving to the Hoover school system in 2005.
She started as an assistant principal at both Greystone and Riverchase elementaries but in January 2006 was dedicated as an assistant at just Riverchase.
Turney said she got into education because of her love for learning.
"I loved going to school and loved being in school and challenging myself to learn new things," she said. "I wanted to pass that onto kids and afford them opportunities to have their own passions and get them excited about learning."
It's like the ripple effect of a pebble in a pond, she said.
"I may not be able to change the entire world, but I certainly want to do something about my little section of it and make it better," Turney said.
As a principal, she loves having the opportunity to work with families and teachers to find ways to do things better, and she feels like it is a job where she is able to make a difference, she said.
She especially loves working at Riverchase Elementary, she said. When she moved from metro Atlanta when her husband got a job in the Birmingham area, she never thought she would find a place she loved as much as she loved Douglasville, Georgia, but "I was so wrong," she said.
"I'm definitely very blessed to work here at Riverchase," Turney said. "There's just a lot of community support. There's a lot of parental support, and the teachers are phenomenal."
Everyone does everything they can to make learning exciting for the kids, she said.
Alice Turney Riverchase Elementary School Dianne Baggett Blue Ribbon School of Excellence Alabama Association of Elementary School Distinguished Principal award Hoover City Schools Foundation Council for Leaders in Alabama Schools


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White canes a welcome gift for sight-impaired children
Victoria White Cane Society provides canes to school-aged children, an expense not covered by health plans
CNIB orientation and mobility specialist Mary Kay Kennedy
Ann Jackson tears up as she recalls meeting dozens of partially sighted and blind children for the first time at the CNIB holiday celebration last December.
"It made me very happy that I was there and our club was able to help," she said.
Since she started losing her vision 12 years ago, Jackson has become an aggressive advocate for the blind as president of the Victoria White Cane Society, a non-profit support group for people dealing with vision impairment.
The society, which celebrates its 66th anniversary this month, recently partnered with the CNIB's Victoria office to provide canes to school-aged children, an expense not covered by provincial health plans.
"Most of the children that we see have some vision, but many of the children need assistance," said Mary Kay Kennedy, CNIB orientation and mobility specialist.
One of the key mobility tools for children is a cane, Kennedy said, used by children as young as two-and-a-half.
"A cane is designed to grow with your size. So, as the children get taller, the cane gets taller," she said.
Vision can also change as a child gets older, making it necessary to purchase different types of mobility canes along the way.
Kennedy is one of three specialists who provides assessment and mobility services to the Island's estimated 75 partially sighted and blind students.
"That number actually might be greater than that, those are the children we know of at CNIB in the school system," she said.
Jackson said her cane serves as a safety belt, helping her navigate her daily activities with more confidence.
"It's saved my life many times," she said. "It's not just for the children, it's for anybody who is blind or partially sighted."
The Victoria White Cane Society will celebrate it's 66th birthday at the CNIB on Wednesday (Sept. 12), providing cake and musical entertainment for its roughly 50 members.
For more information on the society, call 250-385-3707. To donate to the CNIB, visit cnib.ca.
PHOTO GALLERY: 145th Saanich Fair
Panorama elder program growing
'We are unarmed, they have guns,' protesters chanted on Tuesday morning


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People of Ukraine
Russian Aggression
Hybrid War
Peace to Ukraine
Putin's new "passport policy": facts and consequences
2019/05/08 - 09:23 • Crimea, War in the Donbas
Russia's new "passport policy" will be applied to all citizens that were born and had permanent residence in Crimea.
In late April, Russian President Vladimir Putin signed a decree on a simplified procedure allowing residents of the so-called "DNR/LNR republics" to obtain Russian passports. Ukrainian President Petro Poroshenko called this step Russia's preparation for the "annexation of Ukraine's Donbas and the creation of a Russian enclave in Ukraine". However, Moscow's imperial plans did not end with this decree.
On May 1, Putin signed a new decree – "On defining categories of persons entitled to apply for citizenship of the Russian Federation for humanitarian purposes". One of these categories included Ukrainian citizens who were born and had permanent residence in Crimea and Sevastopol, but had left after the invasion and occupation of the Crimean peninsula; the decree includes their children, spouses and parents.
Kseniya Kirillova (opinion)
It can be assumed that Putin views native Crimeans as potentially loyal people who may become "agents of influence" on mainland Ukraine. He believes that many Crimeans who, due to different circumstances, no longer resided in Crimea as of March 2014, would not have refused a Russian passport. Having acquired the coveted document, these newly minted "Russian citizens" and their families can return to their places of permanent residence, where they will act as yet another "fifth column".
Following Zatulin's* precepts
*(Konstantin Fyodorovich Zatulin is a Russian politician, first deputy chairman of the committee of the State Duma on CIS Affairs and relations with Russian nationals abroad. On June 6, 2006, Konstantin Zatulin was banned from entering Ukraine. The Ukrainian government accused Zatulin of invoking ethnic violence and working against the territorial integrity of Ukraine. In July–August 2008, he was again declared persona non grata in Ukraine-Ed.)
In fact, Russia's new passport policy did not come as a complete surprise. On April 22, as soon as the preliminary results of the second round of the presidential elections in Ukraine were announced, the first deputy chairman of the Russian State Duma Committee on CIS Affairs Konstantin Zatulin appeared on "60 minutes" – a talk show on Russia 1 channel – where he stated firmly that, regardless of Zelensky's policy, Russia must "fulfill its promise to the Donbas people" and grant Russian citizenship not only to residents of the occupied territories, but also to all Ukrainian citizens, without them having to renounce Ukrainian citizenship. Two days after that announcement, Putin signed the decree concerning Donbas residents, and on April 27 he declared that he was thinking about issuing Russian citizenship through a simplified procedure.
Experts at the Russian Institute for Strategic Studies (RISS), previously a division of the Foreign Intelligence Service, and now formally subordinate to the Presidential Administration, went even further:
"Moscow can even apply this simplified procedure on the territory of other states. After all, the Russian-speaking foreign diaspora is large and numbers about 40 million people. Such a decision will be carried out within the framework of a generally accepted practice. " suggested Sergei Galiev, an analyst at RISS.
In turn, president-elect of Ukraine, Volodymyr Zelensky advised the Russian authorities not to waste time trying to tempt citizens of Ukraine with Russian passports.
"Perhaps some people may do it in order to earn more money or escape from criminal investigations… We know perfectly well what a Russian passport actually means – the right to be arrested for taking part in peaceful protests, and the right not to have free and competitive elections…" said Zelensky in his statement.
Zelensky added that, as an example of democracy for post-Soviet countries, Ukraine was ready to extend protection and Ukrainian citizenship "to anyone ready to fight for freedom".
Russia's "passport aggression" and its consequences
Paul Goble, well-known American expert on Eurasia and former analyst for the CIA and the US State Department says that Putin's decision will create problems primarily for Moscow.
"I think this decision will cost Putin dearly. In order to obtain Russian citizenship, a person must reside on the territory of the Russian Federation, and very few people will decide to actually move there. The people who go for this, will move to other parts of Russia and create bigger problems there."
Paul Goble. Photo: delfi.ee
However, Goble believes that Putin is using this decree to test the ground for more serious measures, such as granting Russian citizenship to all citizens of former Soviet countries, which might create many more problems. He also thinks that the United States and Germany might be included in the "other states" that RISS expert Sergei Galiev mentioned.
"I think Putin may go for it, because he clearly does not like the fact that so many ethnic Russians have obtained citizenship in these countries, that most of them will never come back and will be lost forever for Russia. Furthermore, their children often don't even try to obtain Russian citizenship."
Evgeniy Gontmakher, professor at the Faculty of Social Sciences at the Higher School of Economics in Moscow, believes that finding thousands of "new citizens" will not create significant problems for the Russian budget.
"This next year and in the following years, it might actually reach about 100 billion… not in one year, of course, but in a few years. This won't be at all critical for us, and will not put into question the fulfillment of our obligations to Russian pensioners."
Russian propagandists from the News Front information agency even stated that for Russia the anticipated influx of about 4.5 million new citizens will be a "mere drop in the ocean", while for Ukraine it will lead to a "national catastrophe", and the country will simply "cease to exist".
However, let's look at more realistic prospects and not dwell on the Kremlin's long-term dreams about the collapse of Ukraine. At a meeting with representatives of the Russian diaspora in California, the famous Russian satirist, journalist and playwright Viktor Shenderovich suggested that if Putin cannot implement the scenario of Belarus' accession to Russia, the only way for him to remain in power after 2024 will be through imposition of martial law. Shenderovich also warned of the following:
"Now that he's actively distributing Russian passports abroad, he'll have a new excuse for war under the guise of "protecting Russian citizens.".
Translated by: Christine Chraibi
Source: Qha.ua
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Tags: Crimea, Donbas war (2014-present), Russian passports, Vladimir Putin
About the Source
Kseniya Kirillova
Kseniya Kirillova is a Russian journalist that focuses on analyzing Russian society, political processes in modern Russia and the Russian-Ukrainian conflict. She writes for Radio Liberty and other outlets and is an expert of the Center for Army, conversion, and disarmament studies and the Free Russia foundation.
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Wines of great growth on the hills of Adro, a Chardonnay and the noble Pinot Nero of Franciacorta, acquire fascination and capture the attention. The perfume and the immediate freshness of the bubbles rejuvenate the soul and the palate. The constancy in the first months of fermentation in steel tanks followed by a long period of rest in magical as beautiful and refined refermental bottles, is refined and matures in the silence of the Cellar. An intriguing experience, let yourself be conquered by bubbles.
The Sommelier: A Franciacorta Brut, the blend of Chardonnay and Pinot Noir, a golden yellow color with greenish reflections. Brilliant sparkling wine with citrus aromas and complex flavors. I have a strong, fresh taste, and the prolonged stay on the lees has made it fragrant and variegated with aromas of dried fruit and dried figs. Balanced sparkling wine with great body with alcohol 12.5% vol.
The bubbles are very fine and refined elegance. The finish is long and in perfect balance.
Suggested pairings: Let yourself be taken by a very versatile sparkling wine, excellent with the whole meal and served with seafood salads.
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