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TUNIS – 27 July, 2017: On 27 and 28 July, Libyan authorities and civil society organisations, UN entities and other stakeholders have gathered to discuss gaps and opportunities in victim assistance (VA) during the Workshop on Victim Assistance in the Context of Landmines, Cluster Munitions and Other Explosive Remnants of War in Libya. The initiative is co-organized by the United Nations Mine Action Service (UNMAS), the United Nations High Commissioner for Refugees (UNHCR) and Handicap International (HI), and aims at supporting the Libyan Mine Action Centre (LibMAC) and other Libyan authorities in identifying key stakeholders, activities and gaps in victim assistance in Libya.
During two days of presentations and interactive group work sessions, participants will enhance their knowledge on VA as a pillar of humanitarian mine action, as well their knowledge on how to undertake humanitarian responses and development actions on issues relating to health, education, labour, and social welfare.
"I'm looking forward to joining this seminar, as I believe it's a good opportunity to raise the hope inside me and raise the hope of all the war victims. It's true that we are facing a lot of challenges and lack of services, but it makes us happy when we feel that there are people who are concerned about our rights and working to provide the services that we need. Finally, I'm looking forward to representing all of the victims and participating with all of you in this initiative" said Mr. Saeed Abdelaati Saeed, a survivor whom HI assisted through the provision of a wheelchair.
UNHCR, HI and UNMAS will continue partnering with Libyan stakeholders in order to build on the workshop's outcomes, particularly the identification of key responsibilities, good international practices in accordance with international humanitarian law, different types of impairments and disability models, and suitable inter-ministerial multi-stakeholder coordination mechanisms for victim assistance.


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When you join VIGORESS, you have access to four different teams, all of the time. Most athletes start as an "Athlete-in-Training," and work their way up to more intense teams. Those who've had plenty of training experience might join VIGOR(ESS) Strength Athlete or VIGOR(ESS) Competitive Athlete right away. The choice is always yours, and you can move from one team to the next whenever you'd like to.
VIGOR(ESS) Athlete-in-Training (VAT): Phases 1 & 2 create a 12-24 month program focused on fundamental movements and building a solid base of strength. If you've been out of the gym for at least a couple of years, or never trained before, you'll start here.
Training is 4 days per week.
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Training is 4-5 days per week.
I have really enjoyed the VIGORESS program. As a wellness professional and coach myself, you'd think I would workout all on my own. The truth is, after I had my daughter, I needed some direction, accountability and education. VIGORESS is a program I knew I could trust and best of all…see results. There are so many online platforms to choose from these days and it can be confusing. VIGORESS is female friendly and is for all ability levels. I can't recommend it enough.
I love how the workouts are challenging and varied. I can access them easily on my phone and knowing the entire week helps me plan better. This mom, career woman and coach appreciates how organized and user the friendly each workout is. If you don't know a movement is, there is a video right there to watch to gain a clear understanding of how to perform it properly. I mean, it is a full service program!
I worked for Life Time for 18 years and had the opportunity to sit next to Tom. I found out quickly how much knowledge he has with regards to health, fitness, mind, etc…I knew I could ask him anything and he would have some bit of knowledge to share. It was so great!
In May, I purchased Tom's booklet on KETO and on June 18th, I started on the KETO eating plan. I also signed up with Tom for VIGORESS shortly after that.
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This place puts the "Heat On" in Heaton. And by that I mean that it's a 1st and 2nd floor flat and as we all know, heat rises, so let the suckers downstairs pay for your heating! Lolz. Not really – you too should pay for heating otherwise that's basically stealing. I think.
5 beds – all fabulously lovely – and a kitchen / diner / living area that means you can talk to your friends in the living room and watch Strictly Come Dacing whilst heating up your beans on toast. Or if you're one of them posh students, beans on toast with grated cheese on the top.
If you move into this flat, I'll buy you enough beans to last a month. Your flatmates won't thank me for that, so make sure you stand near a window.


No matter what the weather this Easter, grab your friends and soak up all the fun at Aussie World. The latest attraction is water play area Leak'n Logs. It's a great way to cool off on a hot day and if it's wet you may as well get even wetter and have fun at the same time!
Leak'n Logs is jam-packed with unpredictable water surprises including thunder, wind, lightning and rain, a mist shed, random tipping buckets, intermittent and continuous showers, an outback log waterfall, and irregular dancing water jets.
During her recent visit to Aussie World, guest Jade who is a regular visitor to the theme park said she enjoys coming to Aussie World.
"I came here with my school and for my best friend's birthday party," she said.
Aussie World is an all-weather theme park suitable for all ages. There are rides that will get you soaked, rides that will make you green and dizzy, simulators and slides, bumps and coasters….all for the one great entry price.


text:
Study of Threes 3
Bio-Physiological 3s 1
Bicycle History Course
The Devils Advocate 1
Embellished Dichotomies 1
Language Hearing Cognition 1
Sociological 3s 2
Uncertainty Principle Metaphor
New Communism Preface
Diseases of Impoverization
Trelegy
3s in Physics 2
Threesology Research Journal
Anatomical, Biological and Physiological Threes
(Using a philosophy of "threes" as a tool for dissection and comparative analysis)
~ The Study of Threes ~
http://threesology.org
Anatomical, Biological, Physiological threes page 1
Anatomical, Biological, Physiological threes page 10
Using a "threes analysis tool" when studying anatomy, biology and physiology is particularly easy since there are multiple representative examples, and made more so if we adopt a larger holistic philosophy which takes into account the existence of a 1, 2, 3 sequencing development that in some cases we may define as a maturational scenario involving occasions of fusion. No less, we must apply the philosophy across the spectrum of representative examples and not let our conventional labels create barriers to comparison. We must also view our growing list of examples from multiple venues of information as a inter-linked three (or more) multi-dimensional grid instead of looking at everything in a two-dimensional format. Relevant connections may escape our considerations if we don't apply an expansive perception.
For example, if we look solely (as an isolated model) at the single stranded RNA in terms of an "RNA World" hypothesis and not view it as a numerically expressive segment of a larger developmental scenario followed by a double-stranded DNA, the dissection of both may or may not lead us to identify the presence of a 3 to 1 ratio:
3 main components (Triad) of the Biosphere: RNA~ DNA~ Proteins
RNA:
Predominantly single stranded
3 main types: Messenger- Ribosomal- Transfer (mRNA-rRNA-tRNA), (and "small nuclear" is sometimes included as an additional [3 to 1] type)
3-site tRNA model is well established, 2-site model is dead
(Image source: Revelations of a biological abacus)
DNA: Predominantly double-stranded (Double Helix)
3 polymerases (enzymes): DNA Polymerase I- II- III
3-patterned (triplet) codon system
DNA Triple Helix
http://www.cryst.bbk.ac.uk/BBS/whatis/DNAtriple_gr.html
Three noted forms of DNA:
Biochemistry Topics
As far as we know, or at least have labeled our current understanding of genetics, DNA and RNA are the two nucleic acids which are recognized during this period in humanity's level and type of biological investigation. Also, Depending on how one wishes to classify DNA a two-patterned model may be preferred:
"There are two major types of DNA- Genomic DNA and Mitochondrial DNA"
Types of DNA and RNA
It should be of interest for those who like to comparative wide swaths of information from different subject areas, that the lower values of numbers are used again and again, like an infant that is babbling with "reduplications". One must wonder if the mind of humanity is in a stuttering mode of operation need for survival in an incrementally deteriorating Sun-Earth-Moon complex?
Proteins: Predominant triple conformation structure
3 single polypeptide forms: Primary ~ Secondary ~ Tertiary
3 secondary structure regions: Alpha helix ~ Beta sheet ~ Looped
3 tertiary structure domains: Alpha helix ~ Beta sheet ~ Combination of both
(1 multiple polypeptide structure called Quaternary, is a composite)
3 to 1 ratio: {Adenine~ Cytosine~ Guanine} + Uracil = RNA
3 to 1 ratio: {Adenine~ Cytosine~ Guanine} + Thymine = DNA
(Adenine + Thymine) = 2 hydrogen bonds
(Guanine + Cytosine) = 3 hydrogen bonds
(???) + (???) = 1 hydrogen bond???
3 natural forms of DNA: (A ~ B ~ Z), whose origins are related to the conformation of the sugar 2'-endo/ C 3'-endo and the orientation of the base relative to the sugar (syn/anti):
Biological and Physiological 3s page 2
If the early Earth environment had a high incidence of Ultra-violet radiation, we must consider whether this was good or bad for early life processes. If it were bad, this would then suggest that RNA (or whatever occurred first), would have either been partially or totally immune to the effects. In other words, it would have had a tough "hide" that could not be easily branded by the hot sun. Was the single-stranded RNA more susceptible to mutation while the double-stranded DNA, because of its redundancy, created a more difficult situation for mutagenic appearances? Or does a double strand allow for greater "mixing" variability due to redundancy? However, let us not forget that mutations can and do appear, which permits adaptation to environmental changes. Will increased effects of the Sun due to "global warming" cause an increase in mutations... whether or not we are conscious of them because we are only looking for obvious indications and not subtle ones?
First, let's look at some basic information about mutations:
The causes of mutations
Mutations happen for several reasons:
DNA fails to copy accurately
Most of the mutations that we think matter to evolution are "naturally-occurring." For example, when a cell divides, it makes a copy of its DNA — and sometimes the copy is not quite perfect. That small difference from the original DNA sequence is a mutation.
External influences can create mutations
Mutations can also be caused by exposure to specific chemicals or radiation. These agents cause the DNA to break down. This is not necessarily unnatural — even in the most isolated and pristine environments, DNA breaks down. Nevertheless, when the cell repairs the DNA, it might not do a perfect job of the repair. So the cell would end up with DNA slightly different than the original DNA and hence, a mutation
Evolution 101: The Cause of Mutations
The only mutations that matter to large-scale evolution are those that can be passed on to offspring. These occur in reproductive cells like eggs and sperm and are called germ line mutations.
A single germ line mutation can have a range of effects:
No change occurs in phenotype
Some mutations don't have any noticeable effect on the phenotype of an organism. This can happen in many situations: perhaps the mutation occurs in a stretch of DNA with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affecting the amino acid sequence of the protein.
Small change occurs in phenotype
A single mutation caused this cat's ears to curl backwards slightly.
Big change occurs in phenotype
Some really important phenotypic changes, like DDT resistance in insects are sometimes caused by single mutations. A single mutation can also have strong negative effects for the organism. Mutations that cause the death of an organism are called lethals — and it doesn't get more negative than that.
There are some sorts of changes that a single mutation, or even a lot of mutations, could not cause. Neither mutations nor wishful thinking will make pigs have wings; only pop culture could have created Teenage Mutant Ninja Turtles — mutations could not have done it.
Evolution 101: Mutations
Here's a brief reference to the RNA world hypothesis:
The RNA World and the Origins of Life
To fully understand the processes occurring in present-day living cells, we need to consider how they arose in evolution. The most fundamental of all such problems is the expression of hereditary information, which today requires extraordinarily complex machinery and proceeds from DNA to protein through an RNA intermediate. How did this machinery arise? One view is that an RNA world existed on Earth before modern cells arose.
Image From: The RNA World and the Origins of Life
Cover of Molecular Biology of the Cell
Molecular Biology of the Cell. 4th edition.
Alberts B, Johnson A, Lewis J, et al.
New York: Garland Science; 2002.
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter;
Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson.
NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.
According to this hypothesis, RNA stored both genetic information and catalyzed the chemical reactions in primitive cells. Only later in evolutionary time did DNA take over as the genetic material and proteins become the major catalyst and structural component of cells. If this idea is correct, then the transition out of the RNA world was never complete; as we have seen in this chapter, RNA still catalyzes several fundamental reactions in modern-day cells, which can be viewed as molecular fossils of an earlier world.
Second, let's look at an excerpt of some information about specific RNA responsiveness to Ultra-Violet radiation, in order to get some idea of how RNA interacts:
(Though we don't actually know the exact nature of the RNA which existed billions of years ago nor the intensity of the Sun's effects on the early Earth's environment. We simply make educated guesstimations and create experiments like Stanley Miller and Urey.)
Miller-Urey Bong, 2010
RNA self-cleavage activated by ultraviolet light-induced oxidation
Ascensión Ariza-Mateos, Samuel Prieto-Vega, Rosa Díaz-Toledano, Alex Birk, Hazel Szeto, Ignacio Mena, Alfredo Berzal-Herranz, Jordi Gómez
Nucleic Acids Research, Volume 40, Issue 4, 1
February 2012, Pages 1748–1766
https://doi.org/10.1093/nar/gkr822
A novel UV-C-light-induced ribozyme activity was discovered within the highly structured 5'-genomic regions of both Hepatitis C Virus (HCV) and the related Classic Swine Fever Virus (CSFV). Cleavage is mediated by exposure to UV-C light but not by exogenous oxygen radicals. It is also very selective, occurring at base positions HCV C 79 and CSFV A 45 in some molecules and at the immediately adjacent 5'-positions HCV U 78 and CSFV U 44 in others. Among other reaction products, the majority of biochemically active products detected contained 3'-phosphate and 5'-phosphate-end groups at the newly generated termini, along with a much lower amount of 3'-hydroxyl end group. While preservation of an E-loop RNA structure in the vicinity of the cleavage site was a requisite for HCV RNA self-cleavage, this was not the case for CSFV RNA. The short size of the reactive domains (~33nt), which are compatible with primitive RNA motifs, and the lack of sequence homology, indicate that as-yet unidentified UV-activated ribozymes are likely to be found throughout structured RNAs, thereby providing clues to whether early RNA self-cleavage events were mediated by photosensitive RNA structures.
A significant number of novel functions, many of which have proven to be important in the study of gene function and expression, have been attributed to RNA. This, in turn, has led to speculation among experts regarding the evolutionary role of RNA in early biological systems. Most of these non-canonical functions for RNA molecules take place in specific regions containing higher order and evolutionarily conserved structural elements. NMR and X-ray crystallographic analyses have provided invaluable insight into some of these tertiary structures and, in the case of certain ribozymes, have revealed their active sites. One class of RNA that is particularly rich in non-Watson:Crick interactions is the Internal Ribosome Entry Site (IRES) domain located at the 5'-ends of several single-stranded RNA viruses such as Hepatitis C Virus (HCV) and the related animal pestivirus Classical Swine Fever Virus (CSFV) ( Figure 1 ).
Secondary structure diagrams of the 5'-terminal regions of HCV and CSFV RNAs. Structural organization of the 5'-untranslated region (~400 bases long) of HCV and CSFV RNAs. The main differences between the two viral RNAs are that HCV lacks stem–loop 1b of pestiviruses, and domain II is slightly longer in HCV than in the pestiviruses. A remarkable similarity is the presence of an E-loop structural motif in the stem–loop IIb. ( a ) Bases 1–402 of HCV RNA; ( b ) Bases 1–380 of CSFV RNA. Each RNA's 5'-end is on the left; major common structural domains are depicted as domains I–IV in HCV and I to III in CSFV. Domain I ('I') is bipartite in CSFV ('Ia' and 'Ib'). A UV-crosslinkable element previously described in HCV RNA is marked with a double arrowhead in stem–loop IIa. A characterized E-loop in the immediate vicinity, as well as an analogous motif at the same site in CSFV RNA, is drawn in bold. An uncharacterized internal loop in stem–loop Ib of CSFV RNA is also in bold. The position of AUG is indicated. Arrowheads locate the different transcripts size: 1–130, 1–249, 1–402 and 68–100 for HCV RNA and 1–218 and 34–63 for CSFV RNA. Cleavage site is indicated with an undulate arrow.
It is known that both DNA and RNA chains are affected by direct exposure to ultraviolet (UV) light, which in the case of DNA can result in the formation of pyrimidine dimers or random breaks. The effects of UV light on RNA are relatively unknown, although UV-C irradiation has been shown to induce mainly pyrimidine dimers and uridine hydrates and, less frequently, unspecific chain breaks. Base–radicals are major reactive intermediates of RNA UV-mediated degradation. Using an oligoribonucleotide with a position-induced base–radical model, the mechanism of RNA oxidative degradation has recently been elucidated in two consecutive studies. These studies indicated that the cleavage occurred internal to the initial radical generation site, referred to as intra-nucleotidyl strand scission, and at the adjacent 5' nt, referred to as inter-nucleotidyl strand scission, as a consequence of migration of the radical from its original base to the upstream base. Nevertheless, a part of unspecific degradation it should be emphasized that UV-C can also induce specific covalent bonds between bases in certain RNA regions containing structural motifs known as E-loops, an effect which has been utilized for the structural study of RNAs, including HCV RNA.
The biochemical mechanisms employed by RNA are surprisingly diverse. Nevertheless, the known chemistry promoted by natural RNAs alone is limited to transesterification and phosphate-bond hydrolysis, grouped together as nucleophilic cleavage reactions, or their reverse ligation reactions. One interesting exception, albeit in a DNA model, is the known ability of synthetic DNAs to promote self-repair of crosslinked thymine bases in a UV-dependent manner. It has been shown that a structural element involving four deoxy-G-residues absorbs UV-light activating crosslink repair.
Whilst performing UV-C irradiation experiments on HCV RNA transcripts, we found that, somewhat surprisingly, UV-C site-selectively cleaved HCV genomic RNA in the presence of Mg2+. We report here that UV-induced cleavage corresponds to a self-cleavage event specifically promoted by UV irradiation at a very specific location of HCV RNA. We also report the subset of key experiments which indicate that self-cleavage also occurs in the RNA of CSFV, thus making UV-C-induced self-cleavage a putatively general property of RNA.
This is the first evidence of a structure-dependent UV-activated selective self-cleavage of RNA in the absence of a photosensitizer.
Third, let's now move on to some structural elements of cells:
With respect to cells and the differences between animals and plants, we can begin with generalities and move on to more complex issues, without attempting to foray too deeply into cytological considerations which present specialized research interests. In other words, for the present course of presenting generalities of design, one key difference is to point out the presence of triplet microtubules in the animal cell because of the important role the structure plays.
(A microtubule is a) tubular structure of indefinite length, constructed from globular proteins called tubulins, which are found only in eukaryotic cells. Microtubules have several functions. For example, they provide the rigid, organized components of the Cytoskeleton that give shape to many cells, and they are major components of cilia and flagella (cellular locomotory projections). They participate in the formation of the spindle during cell division (mitosis). They also assist the movement of organelles (e.g., mitochondria), as well as the movement of vesicles from the cell bodies of neurons to the axonal tips and back to the cell bodies.
Source: "Microtubule." Encyclopædia Britannica Ultimate Reference Suite, 2013.
Centriole
A morphologically complex cellular organelle at the focus of centrosomes in animal cells and some lower plant cells. Prokaryotes, some lower animal cells, higher plant cells, and a few exceptional higher animal cells do not have centrioles in their centrosomes. Centrioles typically are not found singly; the centrosome of higher animal cells contains a pair of centrioles (together called the diplosome), arranged at right angles to each other and separated by a distance ranging from 250 nanometers to several micrometers.
Centrioles are typically 300–700 nm in length and 250 nm in diameter. Although they can be detected by the light microscope, an electron microscope is required to resolve their substructure. At the electron microscopic level, a centriole consists of a hollow cylinder of nine triplet microtubules in a pinwheel arrangement (see illustration). Within each triplet, one microtubule (the A tubule) is a complete microtubule, while the others (the B and C tubules) share a portion of their wall with the adjacent tubule. In some cells these nine triplet microtubules are embedded in a densely staining cylindrical matrix that is spatially distinct from the pericentriolar material of the centrosome. Structures found in the lumen or core of the centriole include linkers between the triplets, granules, fibers, a cartwheel structure at one end of the centriole, and sometimes a small vesicle.
The Free Dictionary by Farlex: Centriole
Centrosome
An organelle located in the cytoplasm of all animal cells and many plants, fungi, and protozoa that controls the polymerization, position, and polar orientation of many of the cell's microtubules throughout the cell cycle. There is usually one centrosome per cell, located near the cell's center; it doubles during interphase, so there are two when the cell divides. At the onset of mitosis, each centrosome increases the number of microtubules it initiates. These mitotic microtubules are more labile and generally shorter than their interphase counterparts, and as they rapidly grow and shrink they probe the space around the centrosome that initiated them. When the nuclear envelope disperses, the microtubules extend from the centrosome into the former nucleoplasm where the chromosomes have already condensed. Some of these microtubules attach to the chromosomes, while others interact with microtubules produced by the sister centrosome, forming a mitotic spindle that organizes and segregates the chromosomes. During anaphase, sister centrosomes are forced apart as the spindle elongates, allowing each daughter cell to receive one centrosome to organize its microtubules in the next cell generation.
The shapes of centrosomes differ widely between organisms. The centrosomes of animal cells usually contain a pair of perpendicular centrioles including a parent centriole formed in an earlier cell generation and a daughter centriole formed during the most recent interphase. Centrioles can serve as basal bodies for the initiation of a cilium or flagellum in the cells that make them. They appear to be essential for the formation of these motile appendages, so centriole inheritance by both daughters at cell division is analogous to the transmission of a gene.
Microtubules can be viewed as part of an overall 3-to-1 arrangement of the Cytoskeleton, though reference to "four" major cytoskeletal filaments appears to be a common expression, though one might argue for another pattern such as 2 X 2 or 2 X 1 X 1, depending on how one cares to characterize structure and or functionality. However, instead of using enumeration, one might opt for words such as singularity, bilarity and trilarity instead of quadrilineal.
Four major types of cytoskeletal filaments are commonly recognized: actin filaments, microtubules, intermediate filaments, and septins. Actin filaments and microtubules are dynamic structures that continuously assemble and disassemble in most cells. Intermediate filaments are stabler and seem to be involved mainly in reinforcing cell structures, especially the position of the nucleus and the junctions that connect cells. Septins are involved in cell division and have been implicated in other cell functions. A wide variety of accessory proteins works in concert with each type of filament, linking filaments to one another and to the cell membrane and helping to form the networks that endow the Cytoskeleton with its unique functions. Many of these accessory proteins have been characterized, revealing a rich diversity in the structure and function of the Cytoskeleton.
Source: "Cell." Encyclopædia Britannica Ultimate Reference Suite, 2013.
Note: by adding "septins", we have created a 3 to 1 ratio.
Cytoskeletal elements and architecture
The existence of an organized fibrous array or cytoskeleton in the structure of the protoplasm was postulated in 1928 by Koltzoff. The cytoskeleton can be defined as a cytoplasmic system of fibers which is critical to cell motility. It is dynamic three-dimensional scaffolding contained within a cell's cytoplasm and is made of protein. The ability of eukaryotic cells to adopt a variety of shapes and to carry out coordinated and directed movements depends on the cytoskeleton. The cytoskeleton was known to be unique to eukaryotic cells. Recent research has found cytoskeletal elements in bacteria showing that it has evolved early in evolution. Several proteins that are involved in cell division, cell structure and DNA partitioning have been found to form highly dynamic ring structures or helical ?laments underneath the cell membrane or throughout the length of the bacterial cells. The cytoskeleton can also be referred to as cytomusculature, because, it is directly involved in movements such as crawling of cells on a substratum, muscle contraction and the various changes in the shape of a developing vertebrate embryo; it also provides the machinery for cyclosis in cytoplasm. The main proteins that are present in the cytoskeleton are tubulin (in the microtubules), actin, myosin, tropomyosin and other (in the microfilaments) and keratins, vimenti , desmin, lamin and others (in intermediate filaments). Tubulin and actin are globular proteins, while subunits of intermediate filaments are fibrous proteins. The use of high-voltage electron microscopy on whole cells has helped to demonstrate that there is a highly structured, three-dimensional lattice in the ground cytoplasm. Figure 1 gives an overview of the cytoskeletal system. The primary types of fibers comprising the cytoskeleton are:
Intermediate filaments
They are classified based on their size, function and distribution within the cell. The differences among the three cytoskeletal elements is given in Table 1 and are individually explained in the following subsections.
Cell Structure and Function page 1
Figure 1: The cytoskeletal system
Table 1: Differences among cytoskeletal elements
Biology 301: Human Physiology, Cell Structure and Function
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Updated Posting: Sunday, 13th-May-2018... 6:50 AM
Your Questions, Comments or Additional Information are welcomed:
Herb O. Buckland
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redpajama_set_name: RedPajamaCommonCrawl


An icon in the heart of downtown Sarasota's cultural district, the Sarasota Municipal Auditorium stands out as a premier event space for formal and corporate events. From elegant weddings to business meetings, seminars, and tradeshows, the auditorium offers 10,000 square feet of unobstructed floor space and excellent acoustics.
Built in 1938 and designed in an amazing Art Deco style by Chicago architects Thomas Reed and Clarence A. Martin, the Municipal Auditorium has been painstakingly remodeled to maintain its iconic charm while providing the infrastructure needed for modern events. The tall ceilings and stunning hardwood floors create an elegant aesthetic while enhancing the quality of acoustics. Audio, visual, and lighting equipment upgrades were designed and executed to create the ideal atmosphere for both personal and professional events. The facility boasts a 39-foot ceiling, and seating can be planned to accommodate both small groups and large parties.
We offer our hosts and their guests free on-site parking, in-house concessions, and an ATM located within the facility. The auditorium's location grants easy access to downtown Sarasota's finest theaters, museums, hotels, and restaurants. To help your event run smoothly, we offer a front breezeway for ticket sales or registration along with a staging area for catering.
Our event space offers the versatility of planning your event the way you want, as our clients are free to design the space to suit their specific event needs. With the help of our management team and support staff, you'll enjoy a stress-free planning process that ensures a successful event. Our well-equipped spaces provide the foundation you need for a truly spectacular event. We also offer other facilities that specialize in smaller events. The John Chidsey Bayfront Community Center is a 2,000-square-foot space attached to the west side of the Sarasota Municipal Auditorium. It can accommodate 80 guests for a sit-down dinner or up to 175 with theater-style seating. In downtown Sarasota, our Payne Park Auditorium is 4,000 square feet and is ideal for weddings.
The Sarasota Municipal Auditorium has hosted the Sarasota Youth Orchestra's Debutante Ball, the Miss Florida pageant, and the renowned West Coast Symphony. Within the walls of its modern design hide whispers of the past. From numerous high school proms and elaborate receptions to concerts, fundraisers, trade shows, exhibits, and business meetings, our staff will meet your event needs and exceed all expectations. Theatre, banquet, trade show, and open floor plans are all available.
If you're interested in viewing this dynamic event space in action, consider attending the Vintage & Collectables Fair or contact us to schedule a visit. Let us help you make your next event or expo a success! Inquire online today so that we can get started.
The "John Chidsey" Bayfront Community Center is attached to the west side of the historic Sarasota Municipal Auditorium. This quaint little venue has stunning views of the Sarasota Bay and offers a 2,000 square foot facility that is ideal for dances, church socials, meetings, parties and seminars. The space can accommodate 80 guests for a sit down catered function or up to 175 theater seating style!


When you start your own art business, you quickly learn that when you sell a painting, you have to figure out how you're going to get the artwork to your client. Packaging is really important for two reasons: 1) you want to make a great first impression and 2) you want to protect the artwork when it's being transported.
Most pre-cut mats come in a clear protective sleeve, which can be reused to protect your painting. However, if you have to cut your own mat, you'll want to wrap the painting in a clear protective film. This film can be purchased from most art stores. I always cut out a piece of foam board to add stability and hide the taped painting on the back of the mat board. It creates a nice sandwich effect, placing your painting between the mat board and foam board. Always put the painting face down on the clear protective film so you can tape the edges to the back.
I like to show appreciation to others who support the arts (and me!) My thank you cards and business cards are professionally printed with examples of my artwork and logo. Great looking cards show your level of professionalism and can help showcase your brand. You can include extra business cards so the client can share them with their friends.
Once your artwork is matted and protected, it's time to ship it. I typically send artwork in a box filled with shipping peanuts, bubble wrap or air filled shipping bags.


text:
NCBI MeSH
Disease relevance of Chorion
High impact information on Chorion
Biological context of Chorion
Anatomical context of Chorion
Associations of Chorion with chemical compounds
Gene context of Chorion
Analytical, diagnostic and therapeutic context of Chorion
MeSH Review:
Chorion
Zhang, H. et al., Iatrou, K. et al., Hunter, P.J. et al., Claycomb, J.M. et al., Schulz, R.A. et al., et al.
Gallicchio, Mitola, Valdembri, Fantozzi, Varnum, Avanzi, Bussolino, Patel, Clifton, Chwalisz, Challis,
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We measured total, active, and inactive (pro-) renin in maternal serum, fetal arterial and venous blood, and chorion homogenate in eight normotensive term patients and 18 patients with pregnancy-induced hypertension [1].
The expression of activin beta A subunit and activin receptor type II and type IIB mRNAs was assessed by reverse transcriptase-PCR on specimens of amnion and chorion collected from patients delivering at term or undergoing preterm labor [2].
CONCLUSION: In the near-term ovine fetus, hypoxia induced vascular endothelial growth factor messenger RNA expression in the amnion, chorion, and placenta [3].
PRL resistance developing subsequent to this chorion laeve receptor defect might produce the excessive amniotic fluid volume characteristic of polyhydramnios [4].
The aim of this study was to assess apoptosis of the amnion and the chorion in relation to ROM and chorioamnionitis (CAM) [5].
This phenomenon may reflect the separation of the chorion from the decidual layer of the uterus, with the release of intact or degraded chorionic components of the extracellular matrix into the cervical and vaginal secretions [6].
To determine if the sequences bound by the particles play a role in RNA processing, we have correlated the nascent transcript morphology of Drosophila chorion s36-1 and s38-1 genes with their nucleotide sequences [7].
Clones corresponding to two distinct A1 and A2 chorion genes have been isolated from a cDNA library in Drosophila melanogaster and characterized by hybrid-selected translation and blotting-hybridization analysis [8].
Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene [9].
Mash-2 transcripts are found in the female germ line and in the embryo throughout preimplantation development, but are highly expressed later only in the ectoplacental cone, the chorion and their derivatives in the placenta [10].
Two major developmentally regulated isoforms of the Drosophila chorion transcription factor CF2 differ by an extra zinc finger within the DNA binding domain [11].
Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition [12].
Mutations in the Drosophila dE2F1 and dDP genes affect the origin recognition complex (DmORC) and initiation of replication at the chorion gene replication origin [13].
The role of ACE3 in Drosophila chorion gene amplification [14].
Drosophila origin recognition complex (ORC) localizes to defined positions on chromosomes, and in follicle cells the chorion gene amplification loci are well-studied examples [15].
In a minority of VCAM-1-deficient embryos, the allantois was able to fuse with the chorion, but the allantoic mesoderm was abnormally distributed over the chorionic surface [16].
Furthermore, it inhibits endothelial cell morphogenesis on Matrigel and VEGF-A-dependent vascularization of chick chorion allantoid membrane [17].
Within the placenta, expression was particularly high in trophoblast giant cells but moderate levels were also observed in trophoblast cells of the chorion at embryonic day 8.5, and later in the labyrinth which arises from the attachment of the chorion to the allantois (a process called chorioallantoic fusion) [18].
No statistically significant changes were found in the arachidonic acid content of the other lipid fractions of either amnion or chorion laeve [19].
MMP-9 protein and mRNA were co-localized in amnion epithelium, underlying macrophages and chorion laeve trophoblast and decidual cells after labor [20].
Bromodeoxyuridine, origin recognition complex, and the elongation factors minichromosome maintenance proteins (MCM)2-7 and proliferating cell nuclear antigen were precisely localized, and the DNA copy number along the third chromosome chorion amplicon was quantified during multiple developmental stages [21].
The Hc protein sequence has evolved from a family of more ordinary chorion genes, in large part through fixation of mutations leading to enhanced cysteine content [22].
The mutation of a highly conserved tyrosine residue in the ETS DNA-binding domain of the Elg gene product demonstrates that normal gene function is required for proper follicle cell migration, chorion formation, and nurse cell-chromosome decondensation during Drosophila oogenesis [23].
Cholesterol levels were found to be significantly higher in female twins when data was combined over chorion type [24].
We have previously shown that one type of high-cysteine silkmoth chorion protein (Hc-A) has evolved from the A family of chorion proteins by radical modifications of the NH2-terminal and COOH-terminal polypeptide arms: most of the arm sequences have been deleted, while short cysteine- and glycine-containing repeats have expanded into long arrays [25].
In the ovary, the sub-nuclear distribution of ORC1 shifts during a developmentally regulated switch from endoreplication of the entire genome to amplification of the chorion gene clusters [26].
The parent construct supported 18- to 20-fold amplification, and contained the 320 bp ACE3, the approximately 1.2 kb S18 chorion gene and the 840 bp ori-beta [27].
Antibody to the origin recognition complex subunit 2 (ORC2) recognizes large foci that localize to the endogenous chorion gene loci and to active transgenic constructs at the beginning of amplification [27].
Combined activities of Gurken and decapentaplegic specify dorsal chorion structures of the Drosophila egg [28].
E2f2 mutant females display a 50% reduction in chorion gene amplification, and lay poorly viable eggs with a defective chorion [29].
Sequence analysis of the 5' end of the E1 gene has identified an intron near the end of the signal peptide-encoding region, a feature shared with other chorion genes [30].
A system of nomenclature for all chorion proteins is presented, based on their separation on sodium dodecyl sulfate and isoelectric focusing gels [31].
The basal output of 13,14-dihydro-15-keto-PGF2alpha was lower in placenta or chorion collected at spontaneous labor than in that obtained at elective cesarean section [32].
In situ hybridization indicated that the expression of both PGHS mRNAs increased in the epithelial and the mesenchymal cells of the amnion and the chorion laeve at term [33].
The expression of inhibin/activin alpha, beta A, and beta B subunit messenger RNA (mRNA) and the localization of immunoreactive material were evaluated in amnion and chorion collected at term pregnancy by a Northern blot analysis and by immunohistochemistry [34].
Increased fetoplacental active renin production in pregnancy-induced hypertension. Brar, H.S., Kjos, S.L., Dougherty, W., Do, Y.S., Tam, H.B., Hsueh, W.A. Am. J. Obstet. Gynecol. (1987) [Pubmed]
High levels of fetal membrane activin beta A and activin receptor IIB mRNAs and augmented concentration of amniotic fluid activin A in women in term or preterm labor. Petraglia, F., Di Blasio, A.M., Florio, P., Gallo, R., Genazzani, A.R., Woodruff, T.K., Vale, W. J. Endocrinol. (1997) [Pubmed]
Prolonged hypoxia upregulates vascular endothelial growth factor messenger RNA expression in ovine fetal membranes and placenta. Matsumoto, L.C., Bogic, L., Brace, R.A., Cheung, C.Y. Am. J. Obstet. Gynecol. (2002) [Pubmed]
Chronic polyhydramnios is a syndrome with a lactogen receptor defect in the chorion laeve. Healy, D.L., Herington, A.C., O'Herlihy, C. British journal of obstetrics and gynaecology. (1985) [Pubmed]
Increased apoptosis of human fetal membranes in rupture of the membranes and chorioamnionitis. Kataoka, S., Furuta, I., Yamada, H., Kato, E.H., Ebina, Y., Kishida, T., Kobayashi, N., Fujimoto, S. Placenta (2002) [Pubmed]
Fetal fibronectin in cervical and vaginal secretions as a predictor of preterm delivery. Lockwood, C.J., Senyei, A.E., Dische, M.R., Casal, D., Shah, K.D., Thung, S.N., Jones, L., Deligdisch, L., Garite, T.J. N. Engl. J. Med. (1991) [Pubmed]
RNP particles at splice junction sequences on Drosophila chorion transcripts. Osheim, Y.N., Miller, O.L., Beyer, A.L. Cell (1985) [Pubmed]
Chorion cDNA clones of D. melanogaster and their use in studies of sequence homology and chromosomal location of chorion genes. Griffin-Shea, R., Thireos, G., Kafatos, F.C., Petri, W.H., Villa-Komaroff, L. Cell (1980) [Pubmed]
Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene. Spradling, A.C., Waring, G.L., Mahowald, A.P. Cell (1979) [Pubmed]
Essential role of Mash-2 in extraembryonic development. Guillemot, F., Nagy, A., Auerbach, A., Rossant, J., Joyner, A.L. Nature (1994) [Pubmed]
Sequence discrimination by alternatively spliced isoforms of a DNA binding zinc finger domain. Gogos, J.A., Hsu, T., Bolton, J., Kafatos, F.C. Science (1992) [Pubmed]
Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition. Takemura, M., Kimura, T., Nomura, S., Makino, Y., Inoue, T., Kikuchi, T., Kubota, Y., Tokugawa, Y., Nobunaga, T., Kamiura, S. J. Clin. Invest. (1994) [Pubmed]
DNA replication control through interaction of E2F-RB and the origin recognition complex. Bosco, G., Du, W., Orr-Weaver, T.L. Nat. Cell Biol. (2001) [Pubmed]
The role of ACE3 in Drosophila chorion gene amplification. Orr-Weaver, T.L., Johnston, C.G., Spradling, A.C. EMBO J. (1989) [Pubmed]
DNA topology, not DNA sequence, is a critical determinant for Drosophila ORC-DNA binding. Remus, D., Beall, E.L., Botchan, M.R. EMBO J. (2004) [Pubmed]
Targeted disruption of the murine VCAM1 gene: essential role of VCAM-1 in chorioallantoic fusion and placentation. Gurtner, G.C., Davis, V., Li, H., McCoy, M.J., Sharpe, A., Cybulsky, M.I. Genes Dev. (1995) [Pubmed]
Inhibition of vascular endothelial growth factor receptor 2-mediated endothelial cell activation by Axl tyrosine kinase receptor. Gallicchio, M., Mitola, S., Valdembri, D., Fantozzi, R., Varnum, B., Avanzi, G.C., Bussolino, F. Blood (2005) [Pubmed]
Mrj encodes a DnaJ-related co-chaperone that is essential for murine placental development. Hunter, P.J., Swanson, B.J., Haendel, M.A., Lyons, G.E., Cross, J.C. Development (1999) [Pubmed]
Mobilization of arachidonic acid from specific glycerophospholipids of human fetal membranes during early labor. Okita, J.R., MacDonald, P.C., Johnston, J.M. J. Biol. Chem. (1982) [Pubmed]
92-kd type IV collagenase (matrix metalloproteinase-9) activity in human amniochorion increases with labor. Vadillo-Ortega, F., González-Avila, G., Furth, E.E., Lei, H., Muschel, R.J., Stetler-Stevenson, W.G., Strauss, J.F. Am. J. Pathol. (1995) [Pubmed]
Visualization of replication initiation and elongation in Drosophila. Claycomb, J.M., MacAlpine, D.M., Evans, J.G., Bell, S.P., Orr-Weaver, T.L. J. Cell Biol. (2002) [Pubmed]
Origin of evolutionary novelty in proteins: how a high-cysteine chorion protein has evolved. Rodakis, G.C., Kafatos, F.C. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
Ets oncogene-related gene Elg functions in Drosophila oogenesis. Schulz, R.A., The, S.M., Hogue, D.A., Galewsky, S., Guo, Q. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
Effects of chorion type on variation in cord blood cholesterol of monozygotic twins. Corey, L.A., Kang, K.W., Christian, J.C., Norton, J.A., Harris, R.E., Nance, W.E. Am. J. Hum. Genet. (1976) [Pubmed]
DNA sequence transfer between two high-cysteine chorion gene families in the silkmoth Bombyx mori. Iatrou, K., Tsitilou, S.G., Kafatos, F.C. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
E2F mediates developmental and cell cycle regulation of ORC1 in Drosophila. Asano, M., Wharton, R.P. EMBO J. (1999) [Pubmed]
Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori-beta origin elements. Zhang, H., Tower, J. Development (2004) [Pubmed]
Combined activities of Gurken and decapentaplegic specify dorsal chorion structures of the Drosophila egg. Peri, F., Roth, S. Development (2000) [Pubmed]
Drosophila E2f2 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells. Cayirlioglu, P., Bonnette, P.C., Dickson, M.R., Duronio, R.J. Development (2001) [Pubmed]
Nucleotide sequence of an unusual regionally expressed silkmoth chorion RNA: predicted primary and secondary structures of an architectural protein. Regier, J.C., Pacholski, P. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
Silkmoth chorion proteins. Their diversity, amino acid composition, and the NH-terminal sequence of one component. Regier, J.C., Kafatos, F.C., Kramer, K.J., Heinrikson, R.L., Keim, P.S. J. Biol. Chem. (1978) [Pubmed]
Steroid regulation of prostaglandin dehydrogenase activity and expression in human term placenta and chorio-decidua in relation to labor. Patel, F.A., Clifton, V.L., Chwalisz, K., Challis, J.R. J. Clin. Endocrinol. Metab. (1999) [Pubmed]
Prostaglandin endoperoxide H synthase (PGHS) activity and PGHS-1 and -2 messenger ribonucleic acid abundance in human chorion throughout gestation and with preterm labor. Mijovic, J.E., Zakar, T., Nairn, T.K., Olson, D.M. J. Clin. Endocrinol. Metab. (1998) [Pubmed]
Inhibin and activin in human fetal membranes: evidence for a local effect on prostaglandin release. Petraglia, F., Anceschi, M.M., Calzá, L., Garuti, G.C., Fusaro, P., Giardino, L., Genazzani, A.R., Vale, W. J. Clin. Endocrinol. Metab. (1993) [Pubmed]
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Trovare Condominiums look and feel like single family homes so don't let the name fool you. These are wonderful full sized homes with 2 car garages.
Trovare is a community with 86 homes having 2, 3 or 4 bedrooms. Floor plans vary a lot but start at 2400 square feet and go to just over 3200. Built in 2003, standard features where high so upgrades from there wasn'tvery necessary. All the homes are beautiful with kitchens that look custom designed with higher end cabinets and granite or quartz counters.These may not need updating at all if purchased today.Private courtyards are designed, landscaped and maintained by each property owner and the ones I've seen are very nice with waterfalls and private spas.


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Online dating sites and apps have transformed how we find romantic partners. You can now look at the profiles of hundreds of potential partners before even speaking to one of them. With so much choice – there are over dating sites worldwide – finding the best online dating site that gives you great matches can take time and cost a lot too.
1. · 2. eharmony · 3. Match Affinity · 4. Plenty of Fish · 5. Elite Singles · 6. Lovestruck · 7. · 8.
Hailey Bustos has been charged with murder in the death of Adam Hilarie, whom she met on a dating site. There was no reason for Adam Hilarie to believe anything but the obvious: Interested had just been on a promising first date fish a pretty girl. He'd picked her up and they went local in Fish Haven, Fla. A few hours later, the pair ended up at his place, where they talked a bit more and met pound local his roommate.
Bustos sent the single father a text afterward: She'd had a good dating, she wrote to Hilarie, and wanted to meet up the following night at his place. Bustos, 18, brought three woman with her to Hilarie's place, including two career criminals on probation for violent felonies. At least one plenty the pound, police said, had a gun.
When Hilarie, 27, responded to the knock on the door, the men overpowered him and dashed inside, police said.
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The RWS PISTOL MATCH SR offers an optimal mix of hardly noticeable recoil and very good and soft shooting performance. Due to a very uniform burning rate, it produces an identical, slight muzzle jump. There is a reduced short muzzle flash. This cartridge is softer due to less primer ignition material and a new propellant powder.


2016 is a year of many firsts. For London, it will be the first time that AFROPUNK graces the city with its glorious melanin-infused essence. After eleven years at its original location of Brooklyn, New York City, AFROPUNK festival makes the jump across the pond to grace the British with an incredible festival experience, while being positive catalyst for change as well a safe diverse yet inclusive space for all black people. Black excellence from across the United Kingdom and the world will descend on London for the inaugural arrival of AFROPUNK London on Saturday, September 24th, at Alexandra Palace. Bazodee will be there to cover the festival, capturing all the sights and sounds across all our social media channels during the event.
This is not the festival's first time on European shores. AFROPUNK first travelled to Paris, France in 2015 before setting its sights on London the following year. For London, AFROPUNK promises the true festival experience, marrying anti-establishment ethos to diverse musical content and undeniable beauty of black culture. London is a centre of creativity, individuality and diversity, a true centre and showcase of multiculturalism so naturally, AFROPUNK should fit in perfectly. The 2016 AFROPUNK festival lineup is nothing short of electrifying; it boasts the iconic Grace Jones as a headliner act, along with Cakes Da Killa, The Noisettes, Laura Mvula, SZA, Lady Leshurr, and Skinny Girl Diet. Festival-goers will also be exposed to some new acts, such as WORD SOUND POWER who are exclusive to AFROPUNK as well as others like Jorja Smith and the Nova Twins. These diverse local and international performers showcase that while AFROPUNK has moved away from its punk roots in terms of its sound, it surely has not lost the plot in term of multiculturalism and the way that the black experience is widespread, diverse and multifaceted, especially showcased in its musical tastes.
AFROPUNK is the flower that grew and blossomed from concrete. It highlights, celebrates and showcases an alternative scene that emerged from the African diaspora. It combines the art, fashion and music from African, Afro-American and Afropean cultures, combining them in a cohhoblopot (Barbadian term for a stew consisting of variety or medley of ingredients) that is said to produce fantastic sounds and awe-inspiring sights coupled with a phenomenal experience. AFROPUNK will be sure to find a welcome home in London, the perfect ground for the rampant, vivid individuality that is present at every AFROPUNK festival. Black punks, fashionistas, black academics and black thought leaders will all be side by side in this black space with the freedom to express themselves and just be. The thought alone of that is simply refreshing. Inclusivity and diversity coupled with individuality, black self expression and black culture will all be the order of the day tomorrow at AFROPUNK London, and from all accounts it is not a festival to be missed.
Want to know more? Make sure to check out the AFROPUNK website.


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Lost Worlds share video for latest single "What You Say"
Lost Worlds are a noisey rock outfit from the wilderness of Australia, specifically Queensland. They formed around two years ago back in 2016, with their merging two different styles, with early 2000's Garage-Rock mixed with video game style electronica, and you can easily here that in their latest offering, "What You Say", which is lifted from their debut LP. It sounds like it's right from a FIFA Soundtrack, it's urgent with an abundance of hooks and keeps you in with it's memorable choruses.
Speaking about their debut album [Fire Fire] the band's Lee McRae says - " ... Making "Fire Fire" was like throwing it all on black. The gamble that doesn't make sense at all. I wouldn't feel right if we didn't do it and I'd always regret it. This could be the only album that we get to make, so we didn't hold anything back."


The hotel is located in Selcuk, the town formerly known as Ephesus, just below the sixth century castle and only 3.5 km from the ancient site of Ephesus. Restaurants, bars and shops can be found within around 400 metres, and local attractions such as the Basilica of St. John and Ephesus Archaeological Museum are all within a few minutes' walk of the hotel. Local transport passes in front of the premises with links to the large city of Izmir. Izmir-Adnan Menderes International Airport is around 60 km from the hotel. This charming hotel is built in a Turkish mansion style with a main building and 2 blocks set in a lovely garden with an outdoor pool and sun-bathing area. The small reception houses, antiquities and local artefacts create a mystique and unique ambience.


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Just a quick little update to let you all know I will soon be moving over to my new website, www.nomadsontheroad.com. Which is why I haven't updated in a while. But fear not I have been working hard and have lots of great articles ready to update as soon as the site goes live.
I am also planning my next adventure, I have received my IEC visa for Canada and will head out to the second largest country in the world in October. A year of mountain and wilderness living awaits me. I will be updating a few times a week from the mountain town of Fernie.