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Category: p38 MAPK
Cells were washed three times with 1 mL of chilly binding buffer and analyzed by circulation cytometry, while described above
Cells were washed three times with 1 mL of chilly binding buffer and analyzed by circulation cytometry, while described above. Calcium mobilization assay Mobilization of Ca2+ from cytoplasmic stores was determined, while described previously [24] using huCCR6-CHO cells. from two self-employed experiments is demonstrated.(TIFF) pone.0157740.s002.tiff (26M) GUID:?EDCB5E09-1D3A-480B-966A-AC4D8CE1F010 S1 Table: Description of the anti-huCCR6 mAbs used in this study. (TIFF) pone.0157740.s003.tiff (1.4M) GUID:?2D4BB1EA-8D3C-45C9-993F-AF38AA7A8E4E S2 Table: Molecular characterization of the generated anti-huCCR6 mAbs. (TIFF) pone.0157740.s004.tiff (1.4M) GUID:?0098DA24-F199-41CE-8869-51B40C1BA9E5 Data Availability StatementAll relevant data are within the paper and its Supporting Info files. Abstract Chemokines and their receptors play an important part in cell trafficking and recruitment. The CCR6 chemokine receptor, selectively indicated on leukocyte populations, has been shown to play a deleterious part in the pathogenesis of various chronic inflammatory diseases and, as such, may constitute Dantrolene sodium a perfect target in the development of immunotherapeutic treatment. However, to day no neutralizing mouse monoclonal antibodies (mAbs) specific for this chemokine receptor have been reported, whereas info on small molecules capable of interfering with the connection of CCR6 and its ligands is definitely scant. Here, we statement the failure to generate neutralizing mouse mAbs specific for human being (hu)CCR6. Immunization of mice with peptides mimicking extracellular domains, potentially involved in CCR6 function, failed to induce Abs reactive with the native receptor. Although the use of NIH-3T3 cells expressing huCCR6 resulted in the isolation of mAbs specific for this receptor, they were not able to block the connection between huCCR6 and huCCL20. Investigation of Dantrolene sodium the anti-huCCR6 mAbs generated in the present study, as well as those commercially available, display that all mAbs invariably identify a unique, non-neutralizing, immunodominant region in the 1st portion of its N-terminal website. Together, these results indicate the generation of potential neutralizing anti-huCCR6 mAbs in the mouse is definitely unlikely to succeed and that alternate techniques, such as the use of additional animal varieties for immunization, might constitute a better approach to generate such a potentially restorative tool for the treatment of inflammatory disease. Intro CCR6 (CD196) is definitely a CC chemokine receptor, involved in sponsor defense and swelling, especially at epithelial surfaces, that has two specific ligands, the chemokine CCL20 and a non-chemokine ligand -defensin-2, an anti-microbial peptide produced by epithelial cells that collection numerous organs [1C9]. CCR6 is definitely expressed in the cell surface of CD4+ interleukin-17 (IL-17)-, IL-22- and TNF–producing T lymphocytes, a human population with strong pro-inflammatory properties referred to as Th17 cells [10C12], as well as all circulating, naive and memory space, but not germinal center, B lymphocytes [13]. CCR6 is also indicated by IL-17 and IL-22-generating innate lymphoid cells [14] and by immature dendritic cells, although its manifestation within the second option cells is lost following their maturation [15]. There is compelling evidence from experimental mouse models, as well as from medical studies in human being, the CCR6/CCL20/Th17 axis is definitely involved in the pathogenesis of Dantrolene sodium various chronic inflammatory and autoimmune diseases, which has been well recorded for Rabbit polyclonal to BMP7 multiple sclerosis and rheumatoid arthritis. In particular, myelin-specific T cell infiltration in the brain was reported to positively correlate with the manifestation of CCL20 in the choroid plexus of humans with multiple sclerosis or mice with experimental autoimmune encephalitis [16]. Moreover, deficient mice are resistant to the induction of disease which is not due to a defect in the differentiation of Th17 cells in brain-draining lymph-nodes after induction of experimental autoimmune encephalitis, but rather the consequence of the failure of these cells to migrate into the inflamed central nervous system [16, 17]. Related results with respect to lymphocyte migration have been acquired in the SKG mouse model of spontaneous experimental arthritis in which a preferential recruitment of Th17 cells to inflamed, CCL20-expressing, synovial bones was observed that may be inhibited having a neutralizing anti-CCR6 antibody [18], whereas polymorphisms in the gene were reported to be associated with rheumatoid arthritis susceptibility [19, 20]. It is important to note that autoimmune, CCR6-expressing, B cells also perform an important part in the pathology of both multiple sclerosis and rheumatoid arthritis. Current biotherapy, specifically focusing on and depleting B cells from your blood circulation with the anti-CD20 mAb Rituximab? was found to result in a reduction of inflammatory mind lesions and medical relapses in individuals with relapsing-remitting multiple sclerosis [21]. Moreover, treatment of individuals with rheumatoid arthritis with Rituximab? also prospects to a significant improvement of their medical indications (Review in [22]). As all functionally mature B cells, like.
The cells were incubated with MTT for 1 h at 37C; the moderate was removed, as well as the blue pigment made by practical cells was solubilized in 100 l/well of 0
The cells were incubated with MTT for 1 h at 37C; the moderate was removed, as well as the blue pigment made by practical cells was solubilized in 100 l/well of 0.5% (w/v) SDS and 25 mM HCl in 90% (v/v) isopropyl alcoholic beverages [29]. Additionally, inhibition of ROS era was evaluated using a luminescence assay for superoxide, or by Amplex Crimson? assay for H2O2 creation, in cell versions expressing particular NOX isoforms. DPI and four analogs (NSCs 740104, 751140, 734428, 737392) highly inhibited HT-29 cell development and ROS creation with nanomolar strength within a concentration-dependent Zamicastat way. NSC 737392 and 734428, which both feature nitro useful groups on the meta placement, got >10-fold higher activity against ROS creation by cells that overexpress dual oxidase 2 (DUOX2) compared to the various other compounds analyzed (IC50 200C400 nM). Predicated on these total outcomes, we tested and synthesized NSC 780521 with optimized potency against DUOX2. Iodonium PLCB4 analogs with anticancer activity, like the initial era of targeted agencies with improved specificity against DUOX2, might provide a book therapeutic method of NOX-driven tumors. proliferation and creation in HT-29 cells. The four preliminary candidate substances that performed optimally based on their solubility and their capability to inhibit tumor cell development and ROS creation were subsequently examined for their results on mitochondrial function and ROS formation (as proven in the tests funnel; Fig. 1D), and because of their NOX isoform selectivity then. A 5th analog (NSC 780521; referred to below) was ready after evaluation from the first four to improve relationship with DUOX2. Substance characterization information are proven below for the 5 business lead compounds; data is certainly available upon obtain the various other analogs. Open up in another home window Fig. 1 Advancement of DPI analogs(A) Buildings of DPI and 35 iodonium-class analogs. The framework for the thirty-sixth analog, chemical substance NSC 780521 (521), is certainly shown in fig. 6A. DPI is certainly shown in vibrant font, as well as the business lead compounds described in today's research are highlighted in greyish. (B) Artificial pathway for the creation of substituted DPI analogs. Reagents: Zamicastat a) I2, KIO3, H2SO4; b) KI. (C) IC50 beliefs for iodonium substance inhibition of HT-29 cell proliferation evaluated using the MTT assay at 48 h. Open up circles indicate materials referred to in the scholarly research. (D) Flowchart demonstrating the verification process of the id of potent iodonium analogs. Open up in another home window Fig. 6 Substance 521The inhibitory ramifications of 521 on HT-29 cell development, whole-cell ROS creation, mobile respiration, and extracellular ROS creation were evaluated using the same strategies referred to above for the various other DPI analogs. (A) Chemical substance framework of NSC 780521. (B, C) Concentration-dependent inhibition of HT-29 cell proliferation after 72-h publicity (B), assessed by MTT assay; and of colony development after 2 h, 6 h, or 10 times of HT-29 cell contact with substance 521 (C). (D) Aftereffect of 24-h treatment with 521 on intracellular ROS creation in HT-29 cells, assessed by analytical cytometry using the redox-sensitive dye CM-H2-DCF-DA. (E) Aftereffect of substance 521 on mobile metabolism pursuing 24-h exposure examined by measuring air consumption prices (OCR) and extracellular acidification price (ECAR), respectively, using the Seahorse Extracellular Flux Analyzer; (F, G) PMA-induced extracellular ROS creation assessed by luminescence assay and Amplex Crimson assay in NOX1 (baseline O2?? creation price = 1.37 10?2 RLU/min/cell) and DUOX2/DUOXA2 overexpression steady HEK293 cells (baseline H2O2 production price = 1.0 10?4 RFU/min/cell), respectively, treated with 521 for 30 min. Data in sections B, C, and E represent the mean SD (mistake pubs) of at least three tests. RLU, comparative light products; RFU, comparative fluorescence products. Dibenziodolium, 3,7-dibromo-, bromide (NSC 740104-T, 104) Mp 202-205 C (decomposes). 1H NMR, DMSO- 9.40-9.39 (d, 1H); 8.68-8.64 (dd, 2H); 8.59-8.57 (dd, 2H); 7.90-7.87 (t, 1H); 7.81-7.79 (t, 1H). Anal. Calcd (C12H7INO2?Cl) C,H,N,Cl,We. Produce: 94 %. Dibenziodolium, 1,9-dinitro-, sodium with bromide (1:1) (NSC 780521, 521) MP 207C209 C (decomposes). 1H NMR, DMSO- 9.02-9.01 (d, 2H); 8.50-8.49 (d, 2H); 8.01-7.98 (t, 2H). Anal. Calcd (C12H6IN2O4 Br) C,H,N,Br,I. Produce: 93%. 2.2 Cell lifestyle HT-29, HL-60, UACC-257, and HEK293 cell lines had been extracted from ATCC (Manassas, VA, USA). Individual HT-29 cancer of the colon cells had been propagated in McCoys 5A moderate supplemented with 10% FBS (Lonza, Walkersville, MD, USA). HL-60 and UACC-257 cells had been harvested in RPMI-1640 moderate formulated with 10% FBS. The stable HEK293 Zamicastat cell range expressing both human DUOXA2 and DUOX2 enzymes was kindly supplied by Dr. William M. Nauseef (College or university of Iowa, Iowa.
For information on statistical strategies, see Desk?S8
July 10, 2021 Gwendolyn Frazier
For information on statistical strategies, see Desk?S8. Diosmin minimal intron retention in genes regulating the cell routine, which was in keeping with fewer RGCs in S-phase and cytokinesis, alongside extended metaphase in RGCs. In every, we discovered that self-amplifying RGCs will be the cell type most delicate to lack of minimal splicing. Together, these findings give a potential explanation of how disruption of minimal splicing could cause microcephaly in MOPD1. cKO mouse by anatomist loxP sites 1090?bp and 1159 upstream?bp downstream from the gene (Fig.?1A, Fig.?S1). Effective targeting from the loxP sites was verified by long-range nested PCR in targeted embryonic stem (Ha sido) cells (Fig.?S1B,C) and additional validated by the increased loss of the wild-type (WT) allele in mice (Fig.?1A). mice, which demonstrated the current presence of the KO allele that was absent in genomic DNA (Fig.?1A). Quantitative PCR (qPCR) for the WT allele demonstrated 50% decrease in mice weighed against mice (Fig.?1B). Intercrossing mice didn't produce mice (Fig.?1C), indicating embryonic lethality. Open up in another home window Fig. 1. U11 reduction in the developing mouse neocortex causes serious microcephaly. (A) Schematic from the floxed (Flx) allele with positions from the loxP sites (blue triangles), with Diosmin agarose gel picture showing PCR outcomes detecting the upstream (still left) and downstream (best) loxP sites. Below is certainly a schematic from the knockout (KO) allele, verified by PCR. See Fig also.?Table and S1?S7. (B) Outcomes of qPCR detecting the WT allele. See Table also?S7. (C) Desk showing genotype regularity of pups created from crosses of mice. (D) Pictures of P0 in the pallium (Gorski et al., 2002). mutant mice, due to collapse from the cortex and lack of the hippocampus (Fig.?1D). To comprehend how this microcephaly precipitated, we searched for to look for the kinetics of U11 snRNA reduction after ablation. hybridization (ISH) for U11 snRNA revealed a decrease in U11 sign (crimson) in the E10 mutant pallium, in accordance with the control (in the control [19.1 fragments per kilobase per million mapped reads (FPKM)] and mutant (20.3 FPKM). Manifestation of was decreased by 59.2% in the mutant weighed against the control, that was further confirmed by quantitative change transcriptase-PCR (qRT-PCR) (Fig.?4B, Desk?S1). The imperfect lack of U11 manifestation in the mutant most likely reflects (1) contaminants of non-(B) and (C). (D) IF for CC3 (green) and H2AX (magenta) in the E12 control (ctrl) and mutant (mut) pallium, with quantification. (E) IF for H2AX (magenta) and p53 (green) in E11 and E12 ctrl and mut sagittal pallial areas, with quantification. Inset pie graphs display the percentage of H2AX+ cells that upregulated p53 (p53+) (remaining) as well Diosmin as the percentage of p53+ cells which were H2AX+ (correct). (F) IF for H2AX (magenta) and Pax6 (green), Tbr2 (green) or NeuN (green), on sagittal parts of the E12 mut pallium, with quantification. (G) IF for p53 (magenta) and Pax6 (green) in the E12 mut pallium, with pie graphs displaying the percentage of Pax6+ cells from Diosmin the p53+ inhabitants (remaining) and of most DAPI+ cells (ideal). Scale CD4 pubs: 30?m. Quantification data are shown as means.e.m. For information on statistical methods, discover Desk?S8. n.s., not really significant; *and ((and C regulate DNA replication and S-phase development (Desk?S5); therefore, disruption of their function most likely leads to DNA cell and harm loss of life in S-phase, which is in keeping with the noticed mobile defects (Figs?4D-E, ?D-E,5G5G and ?and7B).7B). Inadequate DNA harm repair, due to small intron retention in the 13 MIGs regulating this technique (Desk?S6), would donate to DNA harm build up and the next p53 upregulation further. This pathway may underlie the DNA harm seen in the E11 mutant pallium, ahead of p53 upregulation (Figs?4E and ?and7B).7B). Disrupted function of several of the rest of the cell cycle-regulating MIGs, such as for example and cKO mouse All mouse methods were performed based on the protocols authorized by the College or university of Connecticut Institutional Pet Care and Make use of Committee, which ensures adherence towards the U.S. Open public Health Service Plan on the treating laboratory pets. The cKO mouse was generated from the College or university of Connecticut Wellness Center. An individual targeting create was useful to bring in both loxP sites in to the locus in mouse Sera cells (Fig.?S1A). This create included a 5 loxP site 1090?bp through the gene. Instantly upstream from the 3 loxP site was a phosphoglycerine kinase (PGK)-neomycin (Neo) cassette flanked by Frt sites. Additionally, a PGK-diphtheria was contained by this build toxin A (dTA)-bad selection cassette downstream from the 3 arm of homology. This create was electroporated into 129X1/SvJ mouse Sera cells. Effective targeting was confirmed by G418-mediated Diosmin positive selection. Subsequently, nested long-range PCR was used to confirm effective homologous recombination at both 5 and 3 loxP sites (Fig.?S1C). Two Sera.
(DOCX 1215 kb) Acknowledgments We thank the Translational Primary Facility from the School of Maryland Marlene and Stewart Greenebaum Cancers Middle for analyzing cell migration and invasion through xCELLigence real-time cell program
May 20, 2021 Gwendolyn Frazier
(DOCX 1215 kb) Acknowledgments We thank the Translational Primary Facility from the School of Maryland Marlene and Stewart Greenebaum Cancers Middle for analyzing cell migration and invasion through xCELLigence real-time cell program. Funding This extensive research was backed, in part, with the NIH National Cancer Institute (NCI) R01CA212094 and R00CA149178, as well as the School of Maryland Stewart and Marlene Greenebaum In depth Cancer tumor Middle. element in Head and Throat Squamous Cell Carcinoma (HNSCC) in multiple cisplatin-resistant HNSCC cell lines. Strategies We analyzed its molecular hyperlink with SRC and MEK/ERK pathways and driven the efficiency of either MEK/ERK inhibitor PD0325901 or SRC inhibitor Dasatinib on cisplatin-resistant HNSCC inhibition. Outcomes We discovered that ETS-1 protein appearance levels in most cisplatin-resistant HNSCC cell types had been greater than those within their parental cisplatin delicate partners. Great ETS-1 appearance was within patient-derived, cisplatin-resistant HNSCC cells. While ETS-1 knockdown inhibited cell proliferation, migration, and invasion, it might re-sensitize cells to cisplatin treatment even now. Interestingly, previous research show that MER/ERK pathways could regulate ETS-1 through its phosphorylation at threonine 38 (T38). Although virtually all cisplatin-resistant HNSCC cells we examined demonstrated higher ETS-1 phosphorylation amounts at T38, we discovered that inhibition of MEK/ERK pathways using the MEK inhibitor PD0325901 didn't stop this phosphorylation. Furthermore, treatment of cisplatin-resistant HNSCC cells using the MEK inhibitor totally obstructed ERK phosphorylation but ML418 didn't re-sensitize cells to cisplatin treatment. Furthermore, we discovered that, in keeping with ETS-1 boost, ML418 SRC phosphorylation elevated in cisplatin-resistant HNSCC, and treatment of cells using the SRC inhibitor, Dasatinib, obstructed SRC phosphorylation and reduced ETS-1 appearance. Importantly, ML418 we demonstrated that Dasatinib, as an individual agent, suppressed cell proliferation significantly, migration, and invasion, furthermore to success. Conclusions Our outcomes demonstrate which the SRC/ETS-1 pathway has a crucial function and could be considered a essential therapeutic focus on in cisplatin-resistant HNSCC treatment. Electronic supplementary materials The web version of the content (10.1186/s12885-019-5664-7) contains supplementary materials, which is open to authorized users. beliefs <?0.05 were regarded as statistically significant (*ETS-1 protein levels were examined in the indicated cells by Western blot analysis. The tests had been repeated for 3 x. Take note: (S) signifies awareness to cisplatin and (R) signifies level of resistance to cisplatin. ETS-1 appearance levels were analyzed in cisplatin-sensitive (Individual Identification: 784116) and resistant (Individual Identification: 871537) PDX by Traditional western blot Following, we driven ETS-1 protein amounts in the 5 cell pairs. ETS-1 protein level in individual produced UMSXCC74B cells <a href="http://www.surveyor1.com/history.htm">Rabbit polyclonal to AGAP was higher than that of UMSCC2 cells (Fig. ?(Fig.1b).1b). The three cisplatin-resistant HNSCC cells, including Cal27CP, SCC25CP, and FaDu-CP, also demonstrated much higher appearance of ETS-1 in comparison to their parental partner cells, whereas UMSCC17B-CP demonstrated lower ETS-1 appearance compared to UMSCC17B cells (Fig. ?(Fig.1b).1b). To verify the full total outcomes from lifestyle cells, we wished to examine if ETS-1 appearance in cisplatin-resistant mind and neck cancer tumor tissues is greater than that in cisplatin-sensitive tissue. Tumor lysates from two patient-derived xenografts (PDX) had been acquired from an individual who was not really treated with cisplatin ahead ML418 of surgery and an individual treated with cisplatin before medical procedures. The outcomes demonstrated which the ETS-1 appearance in cisplatin-resistant HNSCC was higher than that in cisplatin-sensitive tissues (Fig. ?(Fig.1c).1c). Our outcomes indicated that ETS-1 protein amounts had been up-regulated in most cisplatin-resistant HNSCC. ETS-1 regulates cell development of cisplatin-resistant HNSCC A prior research by Liu, et al.[18] showed that knockdown of ETS-1 with a siRNA against ETS-1 blocked the signaling and function of platelet-derived development aspect D-chain (PDGF-D). As a result, we wished to see whether ETS-1 also performed a job in cisplatin-resistant HNSCC development utilizing the same ETS-1 siRNA. ETS-1 appearance was knocked down in Cal27CP, SCC25CP, and UMSCC74B cells by ETS-1 ML418 siRNA (Fig. ?(Fig.2a).2a). The amount of cells in ETS-1 knockdown examples was significantly less than control examples three times after siRNA transfection (Fig. ?(Fig.2b).2b). Next, the same variety of cells transfected with nontarget siRNA or siRNA against ETS-1 was seeded in 12-well plates for the colony formation assay. We.
Supplementary MaterialsSupplementary Shape Lgends
Supplementary MaterialsSupplementary Shape Lgends. self-renewing abilities, expressing important ovarian cancer stem cell and epithelialCmesenchymal transition markers, as well as increased CC0651 drug resistance and potent tumorigenicity. Microarray analysis of OVS1-derived sphere cells revealed increased expression of amphiregulin (AREG) and decreased expression of its conserved regulatory microRNA, miR-34c-5p, when compared with the OVS1 parental cells. Overexpression of AREG and decreased miR-34c-5p expression in SKOV-I6 and OVS1 sphere cells were confirmed by PLA2G4A quantitative real-time PCR analysis. Luciferase reporter assay and mutant analysis confirmed that AREG is a direct target of miR-34c-5p. Furthermore, AREG-mediated increase of sphere formation, drug resistance toward docetaxel and carboplatin, as well as tumorigenicity of SKOV-I6 and OVS1 cells could be abrogated by miR-34c-5p. We further demonstrated that miR-34c-5p inhibited ovarian cancer stemness CC0651 through downregulation of the AREG-EGFR-ERK pathway. Overexpression of AREG was found to be correlated with advanced ovarian cancer stages and poor prognosis. Taken together, our data suggest that AREG promotes ovarian cancer stemness and drug resistance via the AREG-EGFR-ERK pathway and this is inhibited by miR-34c-5p. Targeting AREG, miR-34c-5p could CC0651 be a potential strategy for anti-cancer-stem cell therapy in ovarian cancer. Introduction Epithelial ovarian cancer is the most lethal gynecological cancer.1, 2 Its high mortality rate is mainly due to late diagnosis, easy spreading, and rapid development of chemoresistance.1, 2, 3 Cancer stem cells are considered to be in part account for chemoresistance, as well as metastasis and recurrent disease.4, 5, 6 Cancer stem cells are defined as a very small subpopulation of tumor cells possessing the ability to self-renew and differentiate leading to the formation of heterogeneous progeny forming the tumor.4, 7 Although number of unique genes and microRNAs (miRNAs) have been found to regulate ovarian cancer stem cells, effective and clinically applicable inhibitors against ovarian cancer stem cells are yet to be developed.8, 9, 10, 11, 12, 13, 14 Human amphiregulin (AREG) is a glycoprotein composed of 84 amino acids and is one of the ligands for the epidermal growth factor receptor (EGFR), a widely expressed transmembrane protein tyrosine kinase.15, 16 Via binding to EGFR and triggering EGFR signaling, AREG has been reported to have important roles in oncogenesis including inhibition of apoptosis, promotion of proliferation, migration, invasion, angiogenesis, chemoresistance and metastasis through activating various downstream signaling pathways such as MAPK/ERK, PI3K/AKT, sTAT and mTOR pathways.15, 16 Overexpression of AREG continues to be reported in solid tumors including ovarian cancer.15, 16 However, the role of AREG in cancer stemness hasn't been reported. Information regarding rules of AREG manifestation by miRNAs in solid tumors can be scarce.17, 18, 19, 20 The part of miRNAs, a conserved course of small non-coding RNAs comprising 21-25 nucleotides long, in anti-cancer therapy continues to be actively CC0651 pursued lately.21, 22, 23, 24 By binding to the mRNA 3-untranslated region (3UTR) sequences of their target genes, miRNAs have been reported to modulate numerous oncogenes or tumor suppressor genes as well as to positively or negatively regulate cancer stem cells.22, 23, 24, 25 Although several laboratories have explored the suppressor roles of miR-34 family in cancer stem cells of various solid tumors such as colon, breast, pancreas, prostate, glioma and non-small cell lung cancer,24, 26, 27, 28, 29 the role of miR-34 family in ovarian cancer stem cells is still unknown. In present study, we successfully CC0651 enriched ovarian cancer stem-like cells from an established human ovarian cancer cell line (SKOV-I6) and a fresh.
Supplementary MaterialsS1 Fig: Gating strategy of (A) Compact disc4+ and (B) Compact disc8+ T cells
Supplementary MaterialsS1 Fig: Gating strategy of (A) Compact disc4+ and (B) Compact disc8+ T cells. T-cell area.(EPS) pone.0150826.s002.eps (9.3M) GUID:?316E7225-7EF6-4C58-9C92-63F88BF3401F S1 Desk: T-cell differentiation position before kidney transplantation in individuals with or without rejection inside the first three months. (DOCX) pone.0150826.s003.docx (17K) GUID:?04E7C314-793C-4D0A-8BB9-26A2FD6EC27B S2 Desk: Risk ratios for the clinical features with regards to early acute allograft rejection (multivariate evaluation). (DOCX) pone.0150826.s004.docx (14K) Capadenoson GUID:?CF87843F-826F-44FF-A996-8DCBBE508B20 Data Availability StatementAll relevant data are inside the paper and its own Supporting Information documents. Abstract History End-stage renal disease individuals possess a dysfunctional, aged peripheral T-cell system prematurely. Right here we hypothesized that the amount of early T-cell ageing before kidney transplantation predicts the chance for early severe allograft rejection (EAR). Methods 222 Capadenoson living donor kidney transplant recipients were prospectively analyzed. EAR was defined as biopsy proven acute allograft rejection within 3 months after kidney transplantation. The differentiation status of circulating T cells, the relative telomere Capadenoson length and the number of CD31+ naive T cells were determined as T-cell ageing parameters. Results Of the 222 patients analyzed, 30 (14%) developed an EAR. The donor age and the historical panel reactive antibody score were significantly higher (p = 0.024 and p = 0.039 respectively) and the number of related donor kidney transplantation was significantly lower (p = 0.018) in the EAR group. EAR-patients showed lower CD4+CD28null T-cell numbers (p 0.01) and the same trend was observed for CD8+CD28null T-cell numbers (p = 0.08). No differences regarding the other ageing parameters were found. A multivariate Cox regression analysis showed that higher CD4+CD28null T-cell numbers was associated with a lower risk for EAR (HR: 0.65, p = 0.028). In vitro, a significant lower percentage of alloreactive T cells was observed within CD28null T cells (p 0.001). Conclusion Immunological ageing-related expansion of highly differentiated CD28null T cells is associated with a Capadenoson lower risk for EAR. Introduction Loss of renal function leads to retention of uremic molecules and cytokines, which creates oxidative stress and inflammation. [1] The resulting pro-inflammatory uremic environment underlies the dysfunctional T-cell immunity of ABL1 end-stage renal disease (ESRD) patients. [2] The major changes in the peripheral T-cell composition are T-lymphopenia, increased T-cell differentiation and loss of telomere length, the latter indicating a history of enhanced T-cell replication. [3] The T-lymphopenia is largely due to a loss of naive (antigen-inexperienced) T cells, which show signs of increased activation and are more prone to apoptosis. [3] This loss of circulating naive T cells runs in parallel with a decrease in newly formed naive T cells, known as recent thymic emigrants (RTEs, indicating a premature involution of the thymus). In combination with an expanded, more differentiated memory T-cell compartment, this leads to a relatively large decrease in the percentage of circulating naive T cells. [3, 4] The highly differentiated memory space T cells are seen as a a lack of the co-stimulatory molecule Compact disc28, producing them less reliant on co-stimulation to be activated. [5] Furthermore, these cells are recognized to possess a lower life expectancy telomere length with their several cell divisions credited. [3, 6, 7] The uremia-associated adjustments in the structure from the peripheral T-cell area resemble the physiological adjustments in the ageing disease fighting capability of elderly healthful individuals, [8C10] that leads to the idea of ESRD-related early immunological ageing. This is confirmed whenever a mixed evaluation from the thymic result, differentiation position as well as the telomere amount of T cells in ESRD individuals was performed as well as the outcomes were in comparison to healthful individuals over a broad a long time. [3] A regular pattern of early immunological ageing was noticed having a discrepancy of 15C20 years between your immunological age group of T cells of ESRD individuals in comparison to their chronological age group. [3, 11] This prematurely aged T-cell program of ESRD individuals gives at least a incomplete description for the improved susceptibility to attacks [12], decreased vaccination response [13C16], improved prevalence of malignancies [17, 18] and could be considered a non-classical risk element for cardiovascular diseases also. [19C22] A prematurely aged T-cell program resulting in impaired T-cell immunity could also decrease the risk for severe rejection after kidney transplantation, but it has not really been studied systematically. Furthermore, most studies.
Polycythemia vera (PV) is a myeloproliferative disorder mostly associated with mutation
Polycythemia vera (PV) is a myeloproliferative disorder mostly associated with mutation. associated with MPD.[3] However, some patients with classical PV lack mutation.[4] We report a case of young man with recurrent CVST and subsequently diagnosed to have = 5.46-16.20) were raised. Open in a separate window Physique 2 MRI and MR venography brain showing Superior sagittal sinus thrombosis with partial thrombosis of right transverse sinus with a large bleed in left frontal region PDK1 inhibitor causing mass effect and midline shift Patient had comparable episode of headache and vomiting, 3 months back for which he was admitted in a hospital. The patient's NCCT head was done 3 months back which showed multiple hemorrhages in right temporal (largest 67 37 mm), occipital and parietal lobes with associated peri lesional edema and midline shift. CT angiography brain was also carried out 3 months back which showed right transverse and right sigmoid sinus thrombosis with right temporoparietal lobe haemorrhagic infarction with mass effect. The patient was encouraged treatment but he was not compliant. In view of elevated packed cell volume, erythropoietin level was measured and it was low, that is 1.68 (= PDK1 inhibitor 5.4-31 mIU/ml) thus signifying PDK1 inhibitor main polycythemia. Bone marrow aspirate showed leucocytosis with neutrophilia with normoblastic erythroid reaction. mutation was not detected. So, finally the individual was diagnosed as Rabbit Polyclonal to TPH2 (phospho-Ser19) CVST connected with intracranial haemorrhage (ICH) with underlying aetiology of gene.[7] There is no significant difference in the presentation of JAK2-positive and JAK2-negative PV, but JAK2-positive PV has a worse prognosis.[8] Polycythemia causes stasis of blood that result in hyperviscosity leading to the development of thrombosis. Thrombosis of cerebral veins or sinuses results in raised venular and capillary pressure. As local venous pressure rises, there is a decrease in cerebral perfusion causing ischemic injury and cytotoxic edema and capillary rupture culminates in parenchymal haemorrhage.[9] The BCSH criteria are considered the most accurate with the acceptable level of sensitivity and ability to differentiate PV and other causes of erythrocytosis.[10] The management of PV is phlebotomy combined with aspirin. Cytoreductive chemotherapy is recommended in patients in whom phlebotomy is usually poorly tolerated and those with high thrombotic risk.[11] Anti-coagulation (AC) is used almost universally on the rationale of reversing the causal thrombotic process. Owing to the presence of a hemorrhagic element in 40% of CSVT, the administration of anticoagulant treatment still remains controversial,[12] although several studies have exhibited AC treatment to be beneficial rather than hazardous. However, repeating a CT after at least 1 day from onset of symptoms to confirm that ICH is usually regressing or at least not progressing may be advisable before PDK1 inhibitor starting AC.[13] Conclusion Patients PDK1 inhibitor with CVST secondary to PV have a standard worse prognosis compared to various other aetiologies. Hence, this case continues to be provided to sensitize the normal physician towards the normal symptoms which are generally misdiagnosed. Early treatment and diagnosis of CVST can prevent lethal complications. Declaration of affected individual consent The writers certify they have attained all appropriate affected individual consent forms. In the proper execution the individual(s) provides/have provided his/her/their consent for his/her/their pictures and various other clinical information to become reported in the journal. The sufferers recognize that their brands and initials will never be published and credited efforts will be produced to conceal their identification, but anonymity can't be assured. Financial support and sponsorship Nil. Issues of interest There is absolutely no conflicts appealing..
Objectives This study aims to evaluate the partnership between C-reactive protein (CRP)/albumin ratio (CAR) and disease activity, standard of living (QoL), and physical function in arthritis rheumatoid (RA)
Objectives This study aims to evaluate the partnership between C-reactive protein (CRP)/albumin ratio (CAR) and disease activity, standard of living (QoL), and physical function in arthritis rheumatoid (RA). element SKF38393 HCl (RF) was positive in 56.2% from the individuals. The median ESR was 19.0 mm/hour [interquartile array (IQR): 19.median and 5] CRP was 5.6 (IQR: 9) mg/L. The median CAR was 1.3 (IQR: SKF38393 HCl 2.1) and median DAS 28-ESR was 3.6 (IQR: 1.5). There is a substantial but weak relationship between CAR and DAS28-ESR [p<0.001, Spearmans correlation coefficient (rs)=0.327]. Furthermore, CAR was correlated with ESR, CRP, and VAS doctors global evaluation (p<0.001, rs=0.497; p<0.001, rs=0.987, p<0.001, rs=0.401, respectively). Nevertheless, no relationship was noticed between CAR, HAQ, and RAQoL. In the RF positive group, there is a substantial but weak relationship between CAR and RF titers (p=0.016, rs=0.292). Summary We noticed an optimistic but weakened relationship between DAS28-ESR and CAR, ESR, as well as the RF titer. Further research are warranted to research the part of CAR in RA. Keywords: Albumin, C-reactive proteins, standard of living, rheumatoid arthritis Intro Arthritis rheumatoid (RA) is certainly a systemic autoimmune disease seen as a synovial inflammation ultimately resulting in joint deformities. It includes a symmetric polyarticular participation from the tactile hands and foot aswell seeing that certain extra-articular manifestations.[1] Early medical diagnosis and treatment of RA may restrain or decelerate joint harm in up to 90% of patients.[2] Therefore, both early medical diagnosis and strict follow-up are obligatory for stopping irreversible disability. Furthermore to physical evaluation and scientific evaluation, the biomarkers are essential in medical diagnosis and stick to- up of RA. The existing classification requirements of RA partly rely on severe stage reactants (APRs) such as for example C-reactive proteins (CRP), erythrocyte sedimentation price (ESR) and autoantibodies including rheumatoid aspect (RF) and antibodies against citrullinated proteins (ACPAs).[3] Treating to focus on strategy in RA recommends measuring disease activity regularly and changing therapy accordingly to optimize the results. Nes The primary objective is capturing circumstances of scientific remission by evaluating the condition activity with usage of amalgamated indices to monitor the severe nature of the condition. The condition activity rating 28 (DAS28- ESR) may be the most broadly applied device in both scientific studies and daily practice.[4] It includes tender and enlarged joint counts (TJC, SJC) on a complete of 28 joints, patient's global assessment of health and wellness on the 100 mm visual SKF38393 HCl analog size (VASglobal), and ESR. Various other commonly used amalgamated indices consist of Simplified Disease Activity Index (SDAI) and Clinical Disease Activity Index (CDAI).[5] Patient-reported outcome (PRO) measures and evaluation of physical features may also be important adjunct disease activity indicators.[6] Acute stage reactants are important in both diagnosis and follow-up. The inclusion SKF38393 HCl of CRP and ESR into composite indices is usually fully justified by their face and content validity.[7] Of these, serum CRP is a representative APR which is mainly synthesized by hepatocytes under the control of proinflammatory cytokines, particularly interleukin-6 (IL-6).[8] In SKF38393 HCl a study on patients with RA, it was stated that serum CRP was the most useful biochemical marker for evaluation of disease activity among various APR assessments including ESR, haptoglobin, ferritin, and fibrinogen.[9] CRP to albumin ratio (CAR) is a novel inflammatory marker which may not only present the inflammatory aspect, but also the nutritional status. CAR was shown to have a prognostic value in lung cancer with significant correlation to overall survival.[10] In a recent retrospective study on 160 patients with RA and 159 healthy controls, CAR was found to be higher than controls and positively correlated with DAS28-ESR. Therefore, CAR was proposed to be a novel inflammatory marker for monitoring disease activity in RA.[11] As far as we are concerned, the association between CAR and other clinical parameters including physical status and quality of life (QoL) in patients with RA has not been investigated in virtually any cross-sectional style before. Therefore, in this scholarly study, we directed to judge the partnership between disease and CAR activity, QoL, and physical function in RA. Strategies and Sufferers Within this cross-sectional research, 121 sufferers with RA (22 men, 99 females;.
Supplementary MaterialsAdditional document 1: Amount S1
October 15, 2020 Gwendolyn Frazier
Supplementary MaterialsAdditional document 1: Amount S1. assays. Immunohistochemistry and Xenografts analyses were conducted to look for the ramifications of ZBTB16 on tumorigenesis in vivo. NNT1 The precise systems of ZBTB16 had been looked into using American blot further, qRT-PCR, luciferase assay, and co-IP. Outcomes was often downregulated in breasts cancer tumor cell lines in relationship using its promoter CpG methylation position. Recovery of ZBTB16 appearance resulted in induction of G2/M stage apoptosis and arrest, inhibition of invasion and migration, reversal of EMT, and suppression of cell proliferation, both in vitro and in vivo. Furthermore, ectopically portrayed ZBTB16 produced heterodimers with ZBTB28 or BCL6/ZBTB27 and exerted tumor suppressor results through upregulation of ZBTB28 and antagonistic activity on BCL6. Conclusions Low appearance of is connected with it is promoter recovery and hypermethylation of ZBTB16 inhibits tumorigenesis. ZBTB16 functions being a tumor suppressor through upregulating ZBTB28 and antagonizing BCL6. Our results also support the chance of ZBTB16 being truly a prognostic biomarker for breasts cancer. or epigenetic and Palmitic acid [7C10] occasions [11] such as for example non-coding RNAs [12]. Surgery remains the principal treatment choice [13C15], but hasn't seen very much advancement for a few best period. For the time being, developments in non-surgical healing choices have got improved the success prices of sufferers significantly, with a documented 7.3% upsurge in the 5-year success rate in China from 2000 to 2014 [16]. Despite significant improvement in treatment strategies over modern times, prognosis of some breasts cancer types, specifically, triple-negative breast cancer tumor, continues to be unsatisfactory [4, 5, Palmitic acid 7C10]. In-depth analysis on the systems root etiology and pathogenesis should facilitate the id of more dependable diagnostic and prognostic markers that may help out with the introduction of book targeted therapeutic medications to improve individual final Palmitic acid results. Zinc finger and BTB domain-containing 16 (is situated on chromosome 11q23 and is one of the zinc finger and BTB/POZ (poxvirus and zinc finger) domain-containing proteins (ZBTB) family members. The gene provides three transcripts (Ensembl Gene Identification:ENST00000335953, ENST00000392996, ENST00000310883), all encoding useful proteins. The translated proteins includes nine C-terminal Krppel-type sequence-specific zinc finger domains, an N-terminal BTB/POZ domains, and three servings from the RD2 subdomains. The C-terminal zinc finger domains promote binding of sequence-specific DNA to its focus on gene to execute a transcriptional repressor function [18]. N-terminal BTB/POZ domains function in Palmitic acid DNA bicycling, proteins dimerization transformation, and proteins/proteins connections to create multi-protein complexes that play significant assignments [19 physiologically, 20]. The RD2 domains is much less well characterized compared to the BTB/POZ domains although mutations in this area have been proven to affect the transcriptional activity of [21]. ZBTB16 is normally portrayed in a variety of regular tissue broadly, including CNS cells, hematopoietic cells, respiratory epithelial cells, cardiac muscles, and skeletal muscles cells [22, 23]. The proteins is necessary for preserving the self-renewal capability of early progenitor and spermatogonial cells [24, 25]. Prior reports show Palmitic acid that gene is normally under-expressed or silenced in multiple tumor tissue or matching cells in a variety of cancer types, such as for example prostate cancers [26], principal malignant melanoma tumors [27], intrusive malignant mesothelioma [28] extremely, hepatocellular carcinoma [29], lung cancers [30, 31], pancreatic cancers, and thyroid carcinoma [32, 33]. Nevertheless, the specific function of ZBTB16 in breasts cancer remains to become set up. ZBTB27 (BCL6) and ZBTB28 (BCL6B) may also be members from the ZBTB family members. continues to be characterized simply because an oncogene and.
Supplementary Materialsijms-21-04166-s001
Supplementary Materialsijms-21-04166-s001. externally from the collagen fibril. The close closeness from the C-telopeptide as well as the MHC1 site of type I collagen to fibronectin, discoidin site receptor (DDR), and collagenase cleavage domains most likely facilitate the discussion of ligands and receptors linked to mobile immunity as well as the collagen-based Extracellular Matrix. = 40) as well as the distance region can be 1.2 0.2 GPa (= 40). These measurements align with those previously established using the nanoindentation technique (Desk 1). Open up in another window Shape 1 Elasticity and adhesive behavior of collagen dietary fiber dependant on atomic power microscopy (AFM) nanoindentation. (A) cIAP1 Ligand-Linker Conjugates 2 The spot appealing with distance and overlap (OL) had been designated where nanoindentation was performed; (B) Normal force-distance curves of collagen materials documented at overlap and distance zones in indigenous and treated areas, respectively, having a ~20 nm indentation. The storyline can be annotated with the procedure of indentation (iCv) through the AFM tip nearing the sample to indenting and liberating after adhesion. Data through the potent cIAP1 Ligand-Linker Conjugates 2 power curves are accustomed to calculate transverse elasticity and adhesion power; (C) Transverse elasticity coefficient and Youngs modulus ( 0.05. 3.4. X-Ray Diffraction Little Position X-ray diffraction (SAX) set up and data integration had been performed in the Biophysics Collaborative Gain access to Group (BioCAT), at Argonne Country wide Lab, Chicago, IL, USA, as reported [19] previously. Approximately 15 purchases of diffraction from the fibrillar collagen type I meridional design were documented for the control (indigenous) as well as the antibody-labeled rat tail tendons. The amplitudes (rectangular root of strength) of purchases 1C10 (to provide an isomorphous quality of 6.7 nm) were scaled following those reported in Antipova and Orgel 2010 [18]. The indigenous rat tail tendon data had been subtracted through the antibody-labeled rat tail tendons data which difference derivative data combined with native collagen stages were utilized to calculate a notable difference Fourier map of 00L = 1C10 (after Orgel et al. (2000), Antipova and Orgel (2010), Orgel et al. (2014) [3,19,20]). We remember that isomorphous labeling can only just likely happen upon the top of fibril/s which decreases the maximum feasible sign from difference Fourier or Pattersons in accordance with full level of the fibril. 3.5. Molecular Visualization Molecular visualization was performed as referred to [7,9,10,20,27]. Molecular surface types were rendered with crucial and PyMol receptor binding sequences/features color coded for simple reference. The collagen molecular coordinates had been from 3HR2 and OSCAR from 5CJB from the RCSB data source [28]. OSCAR was docked by overlaying molecular coordinates from a collagen-like peptide from 5CJB using the fibril surface area structure made up from 3HR2, as well as the GPO series of 1 microfibril in the collagen fibril framework. OSCAR was rotated across the collagen substances lengthy axis while keeping connection with the binding areas, and energy reduced with PyMols optimize (regional optimization, 1000 measures, MMFF94s, conjugate gradients achieving ?1524 Kcal/mol) to very clear molecular collisions. 4. Immunoglobulin-Like Collagen ReceptorConclusions Previously, it's been observed how the GPO5 repeat series is, unlike additional collagen-receptor sequences Mouse monoclonal to CD34.D34 reacts with CD34 molecule, a 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 43A1 belongs to the class II epitope. * CD34 mAb is useful for detection and saparation of hematopoietic stem cells in the fibril, extremely highly available (Shape 7) [20,29]. Much like GPVI receptor binding, the collagen binding series of OSCAR enables the receptor to dock using the fibril without the obstructions. It cIAP1 Ligand-Linker Conjugates 2 really is interesting to notice, the need for the tyrosine residues from the receptor to binding the GPO series [15]. It really is intriguing a concentration of the few tyrosine residues in the C-terminal telopeptide may be the just place tyrosine residues are located in such focus (if) in the sort I collagen series. Actually, the C-telopeptide can be in a limited turn configuration to create many of these tyrosine residues into one carefully spaced area [2,3]. Nevertheless, the physiological.


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text:
Ponca Tribe of Indians of Oklahoma
Business Committee
Ponca Tribe Tax Commission
Tribal District Court
Burial Assistance
FDPIR (Food Distribution Program)
Indian Child Welfare
Office of Environmental Management
Ponca Pathways Program
Ponca Tribal Housing
Project Eagle
Substance Abuse & Suicide Prevention
Social Development Center
Tribal Youth Program
White Eagle Transit
Victims Services
Ponca Language
White Eagle Health Center
Community Health Rep
Diabetes Program
Injury Prevention Program
Purchased Referred Care (PRC)
Ponca Custom T's & Embroidery
Ponca Enterprise Gaming
Ponca Scholars
Ponca Achievements
Ponca Tribal Member or Descendant Graduates of High school, General Education, College, Graduate School, any Certification, or accomplishment demonstrates a willingness to succeed and overcome any obstacles that may hinder progress. Strengthening the Ponca Tribe lies within our own members in order to work together for the proficiency of advancement. Below is a list of Ponca Member or Ponca Descendant recognitions. It is important to show support of achievement for Tribal Member or Descendant accomplishments.
Dominique "Sache" Primeaux-Shaw
She obtained a Bachelor of Arts in History and a Bachelor of Arts in Political Science from Oklahoma City University and a Masters in Public Administration from the University of Central Oklahoma. Mrs. Shaw was also a former Miss Indian University of Central Oklahoma (UCO).
Graduate of Owasso High School, received a scholarship from OSU-Engineering-OSU School of Music and will be marching with the OSU Marching Band. Daughter of Ceasar Williams Jr and Elizabeth Williams, Granddaughter of Ceasar Williams and Dorcas Kent Williams
Congratulations Tyra Williams on achieving your Bachelor of Science in Health Care Administration from Northeastern State University (NSU) in Broken Arrow, OK and will soon finish her Masters in Health Care Administration. Daughter of Ty and Debbie Williams. Granddaughter of Ceasar Williams and Dorcas Kent Williams. Tyra is the current Tulsa Pow-Wow Princess.
Storm'in Bible
Proud of Storm'in for obtaining an Associate of Arts in Education-Elementary from Northern Oklahoma College. She is the daugher of Jana Grass and Kim Grass. Storm'in is also certified in Human Resources.
Sara Buffalo Head-Bell
She obtained her Bachelor of Science degree in Psychology with a minor in Communications from Oklahoma Wesleyan University. Mrs. Bell is also a certified Yoga Instructor. Daughter of Dwight and Judy Buffalo Head. Granddaughter of Roy and Dorothy Buffalo Head.
Congratulations to Wazhinguda Horinek, member of the International Indigenous Youth Council, on receiving the Robert F. Kennedy Human Rights Award of 2018 in Washington D.C. This award is based on individuals or groups that stand up to oppression at grave personal risk in the nonviolent pursuit of human rights. He is of Ponca and Otoe tribal descent, son of Mekasi Horinek and Francis McIntosh. Grandson of Casey Camp Horinek, current Council Women of the Ponca Tribe of Oklahoma.
American Indian Movement Member Dwain Camp, Ponca Nation Elder provided a Remembrance
Remembrance of WK "73"
Wounded Knee, South Dakota was the site in 1890 of a massacre killing nearly 300 native women and children by the United States military forces. Not a battle as it has often been called but a well documented mass murder of unarmed civilians. This atrocity receives scarcely a footnote, if that, in our nation's history books. It deserves much more.
Wounded Knee S.D. was the historically appropriate site, in 1973, of an occupation by native warriors that also deserves not to be forgotten. February 27th of this year marks the 45th year since the American Indian Movement entered and made their stand for 71 days at this small village. I remember I was there.
As a native man born during the depths of the Great Depression in an Indian Health Service hospital, I was raised on a federal reservation and educated at a Bureau of Indian Affairs boarding school. To add another dimension, immediately after leaving boarding school, I traveled, worked, married, and had children while living in the world of the dominant society thus having one foot in our ancient culture and one foot firmly planted in the new millennium. Through living my dual cultures, I had a birds eye view of a deeply ingrained racial inequality. I came to understand that the United States was built on land stolen by force and by broken treaty. I learned that the European philosophical rationalism of "Doctrine of Discovery" and "Manifest Destiny" simply means, "might makes right." I also learned that our native nations, oppressed for 300 years, ravaged by new diseases to near or complete extinction, survived, greatly diminished in number, only to become victims of United States genocidal policies. The inequities list goes on, long and tragic. Then, in 1973, I joined the new warrior society of native people, the freedom fighters of the American Indian Movement (AIM) at the occupation of Wounded Knee, S.D., ("WK73"). This life changing experience began my 45 year journey down the Red Road of education and the struggles of indigenous activism. I proudly stood with my brothers and sisters at WK73. I proudly fought from the bunkers beside my own brothers Craig Camp and Carter Camp. We all learned and we grew in strength and number to become a part of the unmistakable resurgence of native nations within the United States. This progression from subjugation of a disenfranchised people to today's Native Nations, has been one of the most exciting and important chapters in recent history. Indigenous people, against all odds, with diligence, intelligence, strength and courage, salvaged the remnants of a sovereignty denied for centuries and thus embarked upon the miraculous renaissance of a historically resilient people. The native people of our generation, coming of age in a country with rampant racism, when a President was assassinated and other great leaders murdered, were swept up in a turbulence that did not include easily won social change.
It's been said that the occupations of Wounded Knee in 1973 was possibly the longest civil disorder with armed conflict in the U.S. history since the Civil War. Certainly it brought national attention to the oppression of Indian people and it brought changes in federal Indian policy. The enactment of the Indian Self-Determination Act, the Indian Higher Education Act and later the Indian Gaming Regulatory Act, became the catalysts of change unimaginable before the unprecedented siege. Legions of nameless, native heroes and heroines died or went to prison during those formative years. Many more went on to work the rest of their days, with pride and dignity, for their reborn tribal nations, exercising newly restored treaty rights including self governance.
Importantly, we elders must pass on this knowledge gained and time is not on our side. Our Earth Mother is sick. All mankind is threatened. Modern technology plus man's avarice and greed have caused an impending apocalypse. We, who have always been there, whose bones of untold generations of our ancestors enrich the soil, are the stewards, the caretakers of this land. Joining hands will all kindred spirits, native, and non-native, we can give future generations a place in the sun. Our children must learn how imperative is this truth as we teach continuity of culture, tradition, and our true history. To help overcome five hundred years of generational trauma, historical trauma, we elders must impart this knowledge to our young ones. Giving them a positive ancestral foundation helps engender our children with a strong, positive self-image. A pride in themselves and as natives will help future generations be more capable of making good life decisions. Brother Carter Camp, founder of Oklahoma AIM, said, "As time has passed and I see so many of our young people taking part in a traditional way of living and believing, I know our fight was worth it and those we lost died worthy deaths."
So, we will continue to give thanks to Wakonda.
We will heal with Mother Earth.
And Our Journey Will Continue. It Always Has. It Always Will.
AND WE WON'T FORGET WOUNDED KNEE
Dwain Camp, Ponca Nation Elder
shongaska2@gmail.com
List of Contact Numbers below for departments
and programs within the Ponca Tribe of Oklahoma
Ponca Tribal DirectoryPonca Tribal Directory
20 White Eagle Drive
Ponca City, OK 74601
Copyright © 2018 Ponca Tribe of Indians of Oklahoma. All Rights Reserved.

redpajama_set_name: RedPajamaCommonCrawl


Perfection: the condition, state, or quality of being free or as free as possible from all flaws or defects.
The push was an accident. It was one of those times when you're cautiously walking over something dangerous, and the person behind you grabs you and fake jolts you near the edge. You scream and freeze, but soon relax when you recognize you're still on safe ground.
This scenario is normal for most people.
I, however, have a complete lack of balance, and suddenly found myself falling, and eventually sinking, into a sucking mud-pit from an Alaskan Glacier.
I have to admit, I was legitimately stuck.
Every time I tried (with both hands) to drag each of my legs out of the mud, the vacuum seal of the mud would sink them down even further.
At this point, I was legitimately scared and started to stress out.
I was in fight-or-flight mode. Nothing was more important than getting myself out. All of my energy and attention was focused on myself.
OMG, I really can't get myself out!
But the reality of the situation- in hindsight of course ("20:20")- was that I had help. I wasn't alone.
Sahand put his phone away so he could help hoist me out. With an extra set of hands and some re-angling of my legs, I was able to break the vacuum seal that was keeping me tethered to the mud.
I didn't drown, and I didn't have to find a solution solely on my own.
Safe on the walkway, I was able to let out a big breath and finally laugh!
This Alaskan experience was a great metaphor for the dangers we may fall victim to in our quest for Perfection.
I'm guilty of striving for Perfection. It's what our society obsesses over, and I'm guilty of obsessing over it too.
This is my daily trudge. I convince myself that by following these self-directed guidelines, I will be on my way to achieving Perfection in my physique, health, linguistics, and career.
Things usually go smoothly week to week in a fairly consistent and routine way. That is, until life happens, and something out of your control knocks you off balance.
For me, it was the monthly avenge of being a female. I felt bloated, swollen, and completely out of shape. Clothes I normally had no problem fitting into were suddenly a struggle to button. Even workout shorts were becoming suctioned to my thighs. Talk about discouragement from wanting to hit the gym! Not to mention the added bonus of hormonal emotions…!
I was anxious, discouraged, insecure, and emotional.
I tried to get out of my funk by exercising, but the act of even changing into workout clothes was disheartening. I was self critical of my workout appearance, performance, and muscle soreness during the following days of recovery.
I became self-critical of my eating habits. Sure, I was eating healthy, but my cravings had me snacking throughout the day and late into the night, perpetuating the feeling of lack of control, lack of willpower, and a sense of self-neglect.
Despite my best attempts to beat the bloat, counter the cravings, and persevere through my poor work-out performances, I felt pretty defeated. I needed help.
Date night became my therapy session. It was pivotal in rebuilding my motivation, demolishing my self-hate attitude, and re-energizing my positive outlook on self-care.
The following day, I laced up my sneakers and took a break from my HIIT workouts. I put on my headphones and walked a few miles around the neighborhood listening to some low-key playlists on Pandora.
The weather was sunny and warm with a light and soothing breeze. After a few miles, the effects of endorphins started to creep in with a similar shift in my overall outlook. A little "Zooming Out," a little help from others, and some small changes in my outlook were key to recognizing that my submersion in the Perfection Pit was only transient.
After my walk, I came back to my apartment feeling lighter and happier.
I wanted to use this experience as new motivation for self-growth, positive change, and to build a healthier outlook on my life.
Looking back at the emotional rollercoaster I put Sahand through over the past few days, I had to laugh….
Whatever push Life gives you into the Perfection Pit, recognize that it's only temporary!
Don't allow yourself to wallow, reach out to friends/ family, and don't forget to laugh when you come out on the other side!


text:
Q: Getting strings inside a split string I am loading strings from a text file, eg;
Sunset Blvd 1950.ogg,Sunset Blvd,Paramount Pictures,1950,110,Billy Wilder,4,William Holden,Gloria Swanson,Erich von Stroheim,Nancy Olson

Now I have a class setup that extends from 2 parent classes (Media > Video > Avi/Ogg/etc). And that class holds the following variables;
 public Avi(String title, String fileName, int releaseYear, String studio, String director, String castNames, double runtime, int cast) {
        super(title, fileName, releaseYear, studio, director, castNames, runtime, cast);
    }

Now I load the text file in using a buffer reader and a loop, but heres the problem, the cast names (Which come last in the text file, are also separated with commas but since I am using a splitter already I am not sure how to get every cast member into a simply string such as "Larry Davis,Eddy Murphy,Etc Etc" that can be returned later on. Also using a different splitter for cast names is not an option

A: if your cast starts at William Holden, you can do
line.split(",", 8);

A: I assume that by splitter you mean the String method "split".
If so, does your text file always have the same structure ?
Meaning is there always the same number of elements before the cast names ?
Because the String method "split" can take a second parameter specifying the number of elements to retrieve (cf. link to String API)

A: None of these solutions worked but heres what I came up with that worked:
String castNames = "";
                    int splitLength = split.length - 7;
                    for (int i = 0; i < splitLength; i++) {
                        castNames += split[7 + i] + ",";
                    }
                    Avi avi = new Avi(split[1]/*title*/
                            , split[0]/*filename*/
                            , Integer.parseInt(split[3])/*releaseyear*/
                            , split[2]/*studio*/
                            , split[5]/*director*/
                            , castNames/*castnames*/
                            , Double.parseDouble(split[4])/*runtime*/
                            , Integer.parseInt(split[6])/*cast*/);
                return avi;

A: Having a symbol as the string separator as well as being valid data is not a good idea and results in code that is prone to errors. Of course you can work around that - some people before me have suggested ways to do it - but I strongly recommend that you change your input and remove the ambiguity.

redpajama_set_name: RedPajamaStackExchange


Presenting , most romantic & melodies INSTRUMENTAL music track from the short film "BOKHATE" in the beautiful voice of SAJIB RANA & SALMA, composed by AHMMED HUMAYUN and written & tuned by SWARAJ DEB.
There is only one happiness in this life, to love and be loved. However difficult life may seem, there is always something you can do and succeed at.


text:
I Could Use Other Photos on Tuesdays, But Why?
By Genevieve Burgess | Miscellaneous | January 27, 2015 |
The Chase on GSN at 8:00pm ET. Fourth season premiere. Exciting news, game show fans! A friend of the site will be competing on this tonight, so watch to support your fellow snarky pop culture fan! And seethe with jealousy, obviously.
Parks & Recreation on NBC at 8:00pm ET.
Marvel's Agent Carter on ABC at 9:00pm ET. Who do we have to talk to about a fashion tie-in? Scandal got one. Come on, guys, I look SO GOOD in hats and it is so hard to find proper ones for women these days. The off the rack numbers are often shapeless and too small for my giant alien head.
Justified on FX at 10:00pm ET.
Rebel Without a Kitchen on Cooking Channel at 10:00pm ET. Series premiere. So normally this is where I'd put the synopsis of the show so that you cooking show junkies could figure out if it was your particular brand of food porn, but I got as far as "Join host Matt 'Fidel Gastro' Basile" and went "NOPE!" so you're just going to have to do your own googling on this one. But, you know, how different are any of these food shows? You have like, three models; competitive, kitchen oriented, and restaurant/eating out oriented, and the differences within those models are minimal at best. This is the third kind, so now you know that much.
Genevieve Burgess watched a lot of Carmen Sandiego as a kid and may still hold some fashion dreams regarding red hats and matching coats.
Ladies and Gentlemen, Meet Your New Ghostbusters
Check Out These Amazing Easter Eggs Hidden Inside the 'Fantastic Four' Trailer!


There's no agnosticism that adopting livestock is a assisting business but it has to be taken serious. Adopting livestock is a abounding time job that requires adherence and a lot of investing. You will accept to absorb money on affairs your livestock, feed, equipment, land, activity and veterinarian expenses. But that shouldn't abash you from adopting livestock because the rewards will awning all those costs and accord you profits.
Almost all types of livestock can accompany you profits if done appropriate but in this commodity we will awning how to accession chickens, goats and beasts just to get you started.
Chickens are no agnosticism one of the easiest livestock to accession which is why a lot of humans are adopting them. Organic craven meat and eggs accept a top appeal in the markets. Craven articles can calmly be marketed to bounded aliment and awash to accompany or neighbors.
The nice affair about adopting chickens is that you don't charge a lot of amplitude to accession them and they aswell eat beneath if compared to added livestock. Just body them a simple craven accommodation area they can beddy-bye and lay some eggs. And aswell accomplish abiding you accord them aliment and baptize on circadian bases.
Goat meat is eaten by about 70% of the world's population. Dupe meat is aswell in appeal in the United States which mostly imports the meat. This agency that there is allowance for bounded farmers to accession goats for meat and ample the need.
But not alone can you accession goats for meat, dupe milk is top in appeal as well. Reason getting it has a lot of proteins again cow milk and is acclimated to aftermath a lot of articles like candy, yogurt and soaps. The fur can be awash as able-bodied to aftermath cloths and bags.
Raising goats requires that you aboriginal body a pen for them to blow and be protected. Some agriculture acreage is appropriate as able-bodied for them to eat grass and added plants. Aswell accomplish abiding you accomplish approved checkups on them for illnesses and abundant ones.
There's no agnosticism that beasts accept a ample bazaar not alone in the United States but all over the world. They are almost low-maintenance livestock with their organically aloft beef and milk in top demand.
In adjustment to accession beasts you accept to accept some ample acreage for them to abrade and eat grass. You aswell accept to accumulation them with lots of baptize because they alcohol a lot of baptize not alone to survive but to abstract the aliment they eat.


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» Are different parenting styles an issue after divorce?
Are different parenting styles an issue after divorce?
On Behalf of Laura Dale & Associates, P.C. | Apr 4, 2022 | Child Custody
Each parent has their own style. When you live together as a married couple, you likely compromise your true styles for compatibility with each other. Yet when you divorce, there is less pressure to do so.
Does that mean you can now parent exactly how you want with complete disregard for the other parent's style? Of course not.
You still need to parent together
Co-parenting, as the name suggests, is a team effort. You can certainly gain more freedom to parent in your house in the style you prefer, but if your styles are polar opposites, your child will be confused. Besides, some parental decisions need to be taken together.
How much freedom do you have?
Kids adapt to differences between places pretty well. For example, they know they can talk and behave one way when hanging out with their buddies at the skatepark but instantly switch when they get back home and switch again when they go to Grandma's.
It's the contradictory differences that matter. For example, your co-parent says they need to be home straight from school to do their homework and can only go out on weekends to the skatepark. You, on the other hand, feel it is up to them when they do their homework and if they can balance having fun with their friends every night with keeping on track at school, then good for them.
These sorts of differences need discussing. Otherwise, the parent whose views the child least agrees with may find their kids no longer want to come and stay. It could lead to further problems if that parent accuses the other of failing to uphold the custody schedule.


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Paula Patton Dives Into Her Character for New BET + Original Series 'SACRIFICE'
Jiggy Jones | November 18, 2021
Paula Patton is back as the head woman in charge in the latest BET+ series, "SACRIFICE." In the BET original movie turned series, Patton plays the uncanny role of prominent entertainment lawyer, Daniella Hernandez. Visit streaming.thesource.com for more information A relentless nature is a trait that Patton's character displays throughout the series. Hernandez is on […]
Alfonso Ribeiro Speaks Candidly About Being Rejected From The Black Community
Miss2Bees | August 2, 2021
Alfonso Ribeiro is best known for his role as Carlton Banks on the beloved 1990s sitcom The Fresh Prince of Bel-Air starring Will Smith. Visit streaming.thesource.com for more information Carlton's character is important because he represents a non-stereotypical member of the Black community who isn't considered "Black enough." Ribeiro opened up about being typecast for […]
Cardi B Says She Has A Special Announcement This Week
Nailah Spencer | July 14, 2021
It looks like we can expect news from rapper Cardi B this week. Early Wednesday morning, the rapper posted a video to Twitter that left fans waiting in anticipation. The Bronx rapper made an announcement in a video about a surprise she has in store for fans. Visit streaming.thesource.com for more information "I gotta little […]
Twitter Reacts to BET Awards Best Female Hip-Hop Artist Nominees
Nailah Spencer | May 28, 2021
The BET Awards recently released their nominees for "Best Female Hip Hop Artist". According to the list Cardi B. Coi Leray, Doja Cat, Megan Thee Stallion, Latto, and Saweetie have all been nominated for the award. While all of these artists have had an impact on the hip hop scene this year, there are a […]
BET Awards Returns For An In-Person Event This Summer
One of Black Hollywood's iconic award shows is returning with an in-person audience this summer. Nearly a year after being one of the first official award show to take place during the pandemic, the BET Awards will return next month with a vaccinated audience. Visit streaming.thesource.com for more information The Black entertainment awards ceremony announced […]
BET to Honor DMX with Live Exclusive Coverage of His Homegoing Celebration This Sunday
BET is set to televise and stream BET Remembers DMX, which will include live exclusive coverage of his homegoing celebration on Sunday, April 25 at 2:30p.m. ET on BET and its YouTube channel here. In addition to the homegoing celebration, BET will air special programming that day to remember Simmons including a selection of films […]
[WATCH] True Crime Series "American Gangster: Trap Queens" Premieres Jan. 14 On BET
Sha Be Allah | January 8, 2021
BET announces the return of its hit unscripted series American Gangster: Trap Queens – a true crime series detailing the criminal chronicles of some of America's most notorious female gangsters. Narrated by female hip hop icon Kimberly Jones a.k.a. Lil' Kim, season two presents new and fascinating true stories of fierce and savvy women who hustled hard and solidified their […]
H.E.R., Chris Brown Lead 2020 Soul Train Awards Nominations
The 2020 Soul Train Awards will hit BET on Sunday, November 29, highlighting the best in R&B and Hip-Hop. The 2020 nominations are led by H.E.R. at eight nominations, she is closely followed by Chris Brown at seven. Visit streaming.thesource.com for more information Additional nominations for the show include Beyoncé and Young Thug tied at […]
BET, Nas and Swirl Films to Release New Documentary 'Smoke: Marijuana + Black America'
Shawn Grant | October 28, 2020
BET has announced a new original documentary, Smoke: Marijuana + Black America, which will premiere on November 18 at 10 pm ET. The new documentary is produced by Swirl Films and narrated and executed produced by Nas. Visit streaming.thesource.com for more information The two-hour documentary highlights marijuana's cultural, social, economic, and legal impact on American […]
Quinn Coleman, DJ, Music Executive and Former BET President Debra Lee's Son, Dead At 31
Sha Be Allah | August 18, 2020
According to several confirmed reports, Quinn Coleman, son of longtime BET President Debra Lee, passed away on Sunday. He was 31 years old. Visit streaming.thesource.com for more information The cause of Coleman's death has not yet been confirmed. Quinn, who went by the name DJ Spicoli, served as A&R Senior Director at Capitol Records at […]
Today in Hip-Hop History: Syndicated Hip Hop Video Show 'Rap City' Premiered On BET 31 Years Ago
On this date in 1989, BET debuted Hip Hop's youngest syndicated video show, known best under the name Rap City, which was a spin-off of Rap Week hosted by long time BET VJ Alvin Jones aka "The Unseen DJ." Rap City was an underdog competitor to MTV's Yo! MTV Raps, however, the appeal of Rap […]
BET Awards Viewership Raises by Over 50%
The 2020 BET Awards viewership put up big numbers. The simulcast on BET and CBS led to a 54% viewership increase, TV Line reports. Visit streaming.thesource.com for more information The show brought in just under 2 million viewers on CBS, making a total of 3.7 million viewers across all of ViacomCBS networks. The show was […]
BET Premieres Upcoming Ruff Ryders Docu-Series Coming This Fall
Jiggy Jones | June 30, 2020
"What y'all really want?" The Ruff Ryders docu-series is coming soon. Visit streaming.thesource.com for more information The BET Awards was more than a celebration of the network's 40th birthday. It was a premiere for a number of future projects that Black Entertainment Television is set to produce, including the 5-part Ruff Ryders docu-series. The DMX-led […]
BET Announces Five-Part Docuseries About Master P's No Limit Records, 'No Limit Chronicles'
Miss2Bees | June 29, 2020
BET delivered an epic, social distance-friendly award show Sunday night and set the bar for all virtual shows moving forward. Visit streaming.thesource.com for more information Fresh off the heels of an enjoyable 20th-anniversary ceremony, the network announced their upcoming docuseries called, No Limit Chronicles. The five-part series will be centered around Master P and his […]
Amanda Seales to Host the 2020 BET Awards
Roman White | June 16, 2020
BET has been rolling out their annual awards show on social media revealing the host for this year. The 2020 BET Awards will be hosted by comedian Amanda Seales. Visit streaming.thesource.com for more information The I Be Knowin' comedian posted the news on her Instagram Monday for the show going down June 28th on BET. […]
Jefferson Davis Statue Comes Down in Richmond as America is Forced Into Atonement
Rhett Butler | June 11, 2020
As the protest movement roils on, America's forced atonement is now targeting its greatest sin: slavery. Visit streaming.thesource.com for more information A statue of Confederate President Jefferson Davis was torn down in Richmond, Virginia along Monument Avenue on Wednesday night. The statue of the Confederacy State's President lies in the former capital of the southern […]
[WATCH] BET Founder Bob Johnson Calls for U.S. to Pay $14T in Reparations for Slavery
Sha Be Allah | June 2, 2020
It's about time someone addressed the root of the economic plight that many are protesting around the country today. Visit streaming.thesource.com for more information Black Entertainment Television founder Bob Johnson took to CNBC's Squawk Box to call on the U.S. government to offer African Americans and the descendants of slaves in the United States a […]


Type I fibers are what people tend to think of as slow twitch muscle fibers, whereas both variations of type II are fast twitch muscle fibers. Let's take a closer look at each type of fiber, and what activities they are best suited for.... Type I fibers are what people tend to think of as slow twitch muscle fibers, whereas both variations of type II are fast twitch muscle fibers. Let's take a closer look at each type of fiber, and what activities they are best suited for.
Muscle-fiber types range from larger ones designed for power activities and strength, to smaller and endurance-based slow-twitch fibers. The larger, fast-twitch muscle fibers store a lot of carbohydrates and have physique implications.... Fast-twitch muscle fibers refer to any muscle fiber that contracts relatively rapidly, utilized especially in actions requiring a maximum effort of short duration, such as sprinting while slow-twitch muscle fibers refer to a muscle fiber that contracts relatively slowly and is resistant to fatigue.
The percentage of fast, slow and intermediate twitch muscle fibers varies from person to person. The proportion is determined by genetics but can change with physical conditioning. Certain hormones, such as human growth hormone (HGH), testosterone and thyroid hormones can also stimulate the metabolism and size of muscle fibers.
For instance, someone born with a certain amount of fast twitch and slow twitch fibers can have slow twitch muscle fibers that exhibit some characteristics of fast twitch muscle fibers through training such as sprinting or heavy weightlifting.
And in the case of targeting different muscle fiber types, we've been conditioned to focus on our fast-twitch fibers because of the role they play in getting us bigger and stronger. That's fine, but as a result we've marginalized the usefulness of our slow-twitch muscle fibers and their related energy systems.
Muscles are divided between slow and fast twitch muscle fibers. Slow twitch muscle fibers are called type I fibers, while fast twitch muscle fibers consist of type IIa and type IIb.


While Tropical Storm Emily seems to be lessening in strength and may not effect Florida in any way, the existence of Tropical Storm Emily shows that hurricane season is here and by all estimates, this hurricane season is expected to be active. There are a bevy of available guides that outline hurricane preparedness and what to do before the storm hits but few of these guides outline how to psychologically survive the environmental and financial effects of being hit. The loss of property, personal heirlooms, and independence that stems from surviving a hurricane is devastating enough but they, unfortunately, can be the tip of the iceberg. Many of these stressors outlined in this article are not considered until the situation arises. It is our hope that someone may read this article and find some solace in the guidelines presented.
In this era of seemingly endless multimedia distractions, many the kids of today are ill equipped for the harsh conditions of the mundane outside world. For the parent who just witnessed the irreversible destruction of their home, personal possessions, and other fruits of their lifelong investment, the inane banter of children whining about being bored can add immense unnecessary stress to an already unbelievably stressful situation. The best solution to this problem is a deck of cards. While Uno, Crazy Eights, and other specialized card games exist, in this reality, space is everything so a simple pack of playing cards can be employed for long term entertainment. The versatility of a standard deck of playing cards is unmatched by any specialized cards and can bring a much needed escape from the harsh post storm conditions.
The financial burden of caring for a pet in a devastated economy can wreak psychological havoc amongst pet owners when they find themselves competing for the same food and water resources as their pet. The pet owners surely has the best intention for the well being of the pet but when the worst has happened and the owner is forced to choose between self preservation and pet preservation, often times it is the pet who suffers. The best solution is to prevent this scenario from happening. Find a pet shelter, find a pet boarding facility, or make arrangements to move the pet to a friend or family member's home - in an unaffected area - before the storm hits. The immeasurable psychological damage of this scenario is easy to stave off with simple prevention.
If you are in an affected area, you need to move to a shelter, friend or relative's house, or to a hotel room where available. While the macho concept of protecting the homestead is honorable and appreciated, the lack of food, clean water, and the constant image of loss is too much on even the toughest psyche. Nothing adds insult to injury better than hunger pains and communicable diseases obtained in a disaster. The need or desire to protect your nonfunctioning and completely unusable 60" HD LCD TV is foolish, it can be replaced... pick up and move on. The best defense against loss is photographic or video evidence. BEFORE the approaching storms hits, photo catalog all of your possessions, heirlooms, expensive electronics, and vehicles; scan all sensitive documents and pictures to your PC; and make a text file of all your Internet usernames and password and then burn all this evidence to a robust medium like DVD. The contents of said DVD can be used for insurance claims, restoration or recreation of lost heirlooms, and is small enough to be easily protected and transported. While other backup methods, including remote backup exist and are reasonable, recovery from these methods can fail when there is no Internet activity, no electricity, no working computers, and your overly stressed mind cannot recall your name let alone Internet passwords. This DVD can put your mind at ease.
In a time of disaster the adage of "Cash is king" is never more apparent. All of the modern luxuries of credit cards, debit cards, and electronic bill payment fail when there is no electricity and no ability to withdraw money from an ATM. The solution is cash. You should maintain a cash reserve in your home for emergencies. This reserve does not need to be large, in fact, too much cash would make you a target so a small coin collection or paper currency reserve should be enough to help you get to an unaffected area in order to regroup and survive.
Surviving a disaster is trying enough. The strategies outlined in this article are presented in the hope that this information helps someone who suffers such challenge survive and recover with as little displacement, loss, and psychological scarring as possible.


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Q: input signal out of range error for high resolution on linux My computer is dual boot with a windows10 and an ubuntu. Once I undeliberately striked the case, and a noise started from it. It seemed that this noise is from cpu fan. I restarted the computer, the noise stopped. But after that every time I try to boot on a linux (even live usb) the monitor shows input signal out of range, unless I set nomodeset or boot on a basic graphic configuration.
But the strange thing is that computer can run Windows 10 with high resolution without any problem. I can't understand whether hardware or software has a problem.


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Sports & Recreation in San Francisco, CA
Boat Charters & Rentals
Guns & Gunsmiths
Gymnastics Instruction
Hunting & Fishing Sites
The Pad Studios
1690 Union St, San Francisco, California, 94123
AcroSports
639 Frederick Street, San Francisco, California, 94117
Rematch Sports
1567 Pacific Ave, San Francisco, California, 94109
735 Market St, San Francisco, California, 94103
Bay City Bike Rentals and Tours
501 Bay Street, San Francisco, California, 94133
Satori Yoga Studio
40 1st Street 2nd floor, San Francisco, California, 94105
Brett Larkin Yoga & Myofascial Release
The Eagle Pass
Mitch Lowe Golf
10th Planet Jiu Jitsu San Francisco
333 Valencia St Suit 100, San Francisco, California, 94103
840 Brannan St, San Francisco, California, 94103
Vima Dance Studio
560 3rd St, San Francisco, California, 94107-1805
Informed Body
389 Grove St, San Francisco, California, 94102-4308
Movement For Evolution Pilates
3556 sacramento st, San Francisco, California, 94118
Brio Pilates
3209 Pierce St., San Francisco, California, 94123
SalsaCrazy Mondays
406 Clement Street, San Francisco, California, 94118
San Francisco Marine Products
350 Bay Street #247 A, San Francisco, CA 94133, San Francisco, California, 94133
American School of Dance and American Dance Training Camp
P.O. Box 14922, San Francisco, California, 94114
Spoke & Hub
1042 Columbus Avenue, San Francisco, California, 94133
Laurie Harden Personal Stylist
1177 California Street, San Francisco, California, 94108
World Class Boxing Gym
1943 Mission St, San Francisco, California, 94110
Yoga Garden of San Francisco
286 Divisadero St, San Francisco, California, 94117
Ferry Building Bike Rentals
1 Sausalito - San Francisco Ferry Building, San Francisco, California, 94111
Pioneer Renewer
4501 18th St, San Francisco, California, 94114


Title: Congratulating Bishop Mark C. Tolbert for a successful 2012 KC Black Expo.
Congratulating Bishop Mark C. Tolbert for a successful 2012 KC Black Expo.
BE IT FURTHER RESOLVED that this Resolution be spread upon the Minutes of the Council in testimony thereof and that a copy hereof be presented to Bishop Mark C. Tolbert with the appreciation of the Mayor and Council for the opportunities presented to the community to improve and enrich their lives.


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Katherine Clark
Rep. Katherine Clark's 2016 Report Card
Representative from Massachusetts's 5th District
Serving Dec 12, 2013 – Jan 3, 2021
These statistics cover Clark's record during the 114th Congress (Jan 6, 2015-Jan 3, 2017) and compare her to other representatives also serving at the end of the session. Last updated on Aug 24, 2017. The statistics were updated on Jan 20, 2017 and Aug 24, 2017 to improve how we counted enacted laws. Originally published on Jan 7, 2017.
A higher or lower number below doesn't necessarily make this legislator any better or worse, or more or less effective, than other Members of Congress. We present these statistics for you to understand the quantitative aspects of Clark's legislative career and make your own judgements based on what activities you think are important.
Got bicameral support on the most bills compared to Massachusetts Delegation
The House and Senate often work on the same issue simultaneously by introducing companion bills in each chamber. 6 of Clark's bills and resolutions had a companion bill in the Senate. Working with a sponsor in the other chamber makes a bill more likely to be passed by both the House and Senate.
Those bills were: H.R. 1258: Pet and Women Safety Act ...; H.R. 1462: Protecting Our Infants Act of ...; H.R. 3067: Child Sexual Abuse Awareness and ...; H.R. 3235: Bringing Postpartum Depression Out of ...; H.R. 4043: Higher Education Access and Success ...; H.R. 4599: Reducing Unused Medications Act of ...
Compare to all Massachusetts Delegation (89th percentile); House Sophomores (84th percentile); House Democrats (86th percentile); All Representatives (85th percentile).
Got bipartisan cosponsors on the most bills compared to Massachusetts Delegation
In this era of partisanship, it is important to see Members of Congress working across the aisle. 11 of Clark's 20 bills and resolutions had both a Democratic cosponsor and a Republican cosponsor in the 114th Congress.
Was most present in votes compared to Massachusetts Delegation
Clark missed 0.7% of votes (9 of 1,325 votes) in the 114th Congress. View Clark's Profile »
Compare to all Massachusetts Delegation (0th percentile); House Sophomores (8th percentile); All Representatives (10th percentile).
Introduced the most bills compared to Massachusetts Delegation (tied with 1 other)
Clark introduced 20 bills and resolutions in the 114th Congress. View Bills »
Compare to all Massachusetts Delegation (78th percentile); House Sophomores (56th percentile); House Democrats (62nd percentile); All Representatives (65th percentile).
Held the fewest committee positions compared to Massachusetts Delegation (tied with 1 other)
Clark held a leadership position on 0 committees and 0 subcommittees, as either a chair (majority party) or ranking member (minority party), at the end of the session. For comparison to other Members of Congress, we assigned a score giving five points for each full committee leadership position and one point for each subcommittee leadership position. View Clark's Profile »
Compare to all Massachusetts Delegation (0th percentile); House Sophomores (0th percentile); House Democrats (0th percentile); All Representatives (0th percentile).
Got the 2nd most cosponsors on their bills compared to House Sophomores
Clark's bills and resolutions had 743 cosponsors in the 114th Congress. Securing cosponsors is an important part of getting support for a bill, although having more cosponsors does not always mean a bill will get a vote. View Bills »
Compare to all Massachusetts Delegation (89th percentile); House Sophomores (97th percentile); House Democrats (93rd percentile); All Representatives (93rd percentile).
Joined bipartisan bills the 2nd least often compared to Massachusetts Delegation
Of the 434 bills that Clark cosponsored, 25% were introduced by a legislator who was not a Democrat. View Cosponsored Bills »
Compare to all Massachusetts Delegation (11th percentile); House Sophomores (51st percentile); House Democrats (24th percentile); All Representatives (63rd percentile).
Ranked 4th most liberal compared to House Sophomores
For more, see our methodology. Note that because on this page only legislative activity in the 114th Congress is considered, the ideology score here may differ from Clark's score elsewhere on GovTrack.
Compare to all Massachusetts Delegation (11th percentile); House Sophomores (4th percentile); House Democrats (18th percentile); All Representatives (8th percentile).
Ranked the 9th top leader compared to House Democrats
For more, see our methodology. Note that because on this page only legislative activity in the 114th Congress is considered, the leadership score here may differ from Clark's score elsewhere on GovTrack.
Cosponsored the 54th most bills compared to All Representatives
Clark cosponsored 434 bills and resolutions introduced by other Members of Congress. Cosponsorship shows a willingness to work with others to advance policy goals. View Cosponsored Bills »
Compare to all Massachusetts Delegation (78th percentile); House Sophomores (81st percentile); House Democrats (73rd percentile); All Representatives (88th percentile).
Supported government transparency the 45th most often compared to All Representatives (tied with 21 others)
GovTrack looked at whether Clark supported any of 40 government transparency, accountability, and effectiveness bills in the House that we identified in this session. We gave Clark 5 points, based on one point for cosponsoring and three points for sponsoring any of these bills.
Clark sponsored H.R. 6340: Presidential Accountability Act
Clark cosponsored H.R. 430: DISCLOSE 2015 Act; H.R. 20: Government By the People Act ...
Compare to all Massachusetts Delegation (67th percentile); House Sophomores (81st percentile); House Democrats (70th percentile); All Representatives (85th percentile).
Clark introduced 1 bill that became law, including via incorporation into other measures, in the 114th Congress. Keep in mind that it takes a law to repeal a law. Very few bills ever become law. View Enacted Bills »
Those bills were: H.R. 1462: Protecting Our Infants Act of ...
Most bills and resolutions languish in committee without any action. Clark introduced 2 bills in the 114th Congress that got past committee and to the floor for consideration.
Those bills were: H.R. 1462: Protecting Our Infants Act of ...; H.R. 4599: Reducing Unused Medications Act of ...
4 of Clark's bills and resolutions in the 114th Congress had a cosponsor who was a chair or ranking member of a committee that the bill was referred to. Getting support from committee leaders on relevant committees is a crucial step in moving legislation forward.
Those bills were: H.R. 1258: Pet and Women Safety Act ...; H.R. 1462: Protecting Our Infants Act of ...; H.R. 2602: Prioritizing Online Threat Enforcement Act ...; H.R. 6340: Presidential Accountability Act


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El Seven Sudamericano Femenino del 2010 se disputó en Mar del Plata, Argentina del 5 al 7 de enero. El equipo de Brasil se coronó campeón luego de vencer en la final a Colombia por 17 a 0. Como es habitual se jugó paralelamente al Seven Masculino y además, en esta oportunidad, también con el Seven Internacional de Mar del Plata 2010.

Equipos participantes

 Selección femenina de rugby 7 de Argentina (Las Pumas)
 Selección femenina de rugby 7 de Brasil (Las Vitória-régia)
 Selección femenina de rugby 7 de Chile (Las Cóndores)
 Selección femenina de rugby 7 de Colombia (Las Tucanes)
 Selección femenina de rugby 7 de Paraguay (Las Yacarés)
 Selección femenina de rugby 7 de Perú (Las Tumis)
 Selección femenina de rugby 7 de Uruguay (Las Teras)
 Selección femenina de rugby 7 de Venezuela (Las Orquídeas)

Clasificación

Grupo A

Posiciones

Nota: Se otorgan 3 puntos al equipo que gane un partido, 1 al que empate y 0 al que pierda

Resultados

Grupo B

Posiciones

Nota: Se otorgan 3 puntos al equipo que gane un partido, 1 al que empate y 0 al que pierda

Resultados

Play-off

Semifinales

Semifinales de Bronce

Semifinales de Oro

Finales

7º puesto

Final de Bronce

Final de Plata

Final de Oro

Posiciones finales

Véase también
 Sudamericano de Rugby A 2010
 Sudamericano de Rugby B 2010
 Sudamericano Juvenil A de Rugby 2010
 Sudamericano Juvenil B de Rugby 2010
 Seven Sudamericano Masculino 2010

Referencias

Enlaces externos
  CONSUR - Reglamento de Torneo Campeonato Seven Sudamericano 2010
  CONSUR - Seven Sudamericano e Internacional Mar del Plata 2010
  CONSUR / Seven Sudamericano y Seven Internacional - Resultados

F2010
Rugby
Deporte en Mar del Plata
Deporte en Argentina en 2010
Torneos y campeonatos de rugby de Argentina
Rugby en 2010

redpajama_set_name: RedPajamaWikipedia