text:
← THE ADDAMS FAMILY on Stage Tube
Musicals on Brantley's 2009 Top 10 List →
New Minimalist Production of CAMELOT
The trend towards pared down productions that somehow supposedly enhance the audience's insight into the show continues; this time with a new production of Camelot by the Pasadena Playhouse. From Playbill:
"David Lee…has a fresh and exciting take on the material that will give the show new vitality and a much stronger dramatic focus," stated Pasadena Playhouse artistic director Sheldon Epps. "I am very pleased that we will be collaborating on this timeless musical, which could not be more timely than at this very moment in our country's history."
Lee explained in production notes, "In the previous incarnations of Camelot there has always been an emphasis on pageantry, big sets, stunning costumes with lots of armor and ladies in pointy hats, a large chorus of singers and dancers, funny mythical characters and even a dog; much of this to stunning effect. But even though the story has large philosophical resonance, it really is a rather small tale about the relationship among three human beings – Arthur, Guenevere and Lancelot. A couple of years ago, just for fun, I went through the script and eliminated everything that did not contribute directly to telling their story. What I was left with was the same beautifully written tale, but one that now seemed more direct, clearer and more emotionally accessible. Plus it was shortened enough that I could afford to add back music that is often cut."
At least Camelot is a somewhat flawed musical to begin with, so the adaptation is perhaps more justifiable. I'd be interested to see what is cut and what is restored. I'd also very interested to see how it will be designed. It's ironic: a minimalist production would seem to take the emphasis off of the design elements, but the more minimalist productions you see, the more you realise how much more difficult it is to make the production compelling visually; thus, the role of the designer takes on, perhaps, even more importance than it did before.
Let's hope that David Lee has learned from the mistakes of some recent productions that have embraced this trend, to varying degrees: the current minimalist revival of A Little Night Music, which misses the fuller orchestrations of the original and features a set that perhaps doesn't serve the show as well as it should; Signature's recent Show Boat, which thought it was a big deal because it didn't have a boat, but which ultimately looked ugly and actually didn't reveal any depths of the show that had hitherto evaded the theatrically literate; and the currently running revival of Ragtime, which substitutes the original production's opulence for an approach that supposedly made for a more emotionally honest experience despite its still considerable size, making use of a large, single set that doesn't quite work all the way through and creates some awkward staging moments that Marcia Milgrom Dodge (whose repetitive, lacklustre choreography can't hold a candle to the work done on the original production by Graciela Daniele) can't seem to solve and a cast that doesn't even come close to banishing the memory of the uniformly superior original Broadway company.
So, Mr Lee, I hope you've got those notes: get a good orchestra with great orchestrations, a clever designer and an excellent cast. Otherwise you might find your audiences yearning for "one brief shining moment" from some Camelot of the past instead of being transported to the kingdom you've envisioned for your new "revisal" of this classic show.
This entry was posted in Broadway, Musicals and tagged A Little Night Music, Broadway, Camelot, David Lee, Graciela Daniele, Marcia Milgrom Dodge, Musicals, Ragtime, Show Boat. Bookmark the permalink.
1 Response to New Minimalist Production of CAMELOT
Pingback: Listen to that RAGTIME « Musical Cyberspace


text:
Picture by: robertsharp | flickr
Explainer: How does the Tory leadership election work?
Grace Whitehouse
Following the UK prime minister Boris Johnson's resignation, the conservative party is in the process of electing its newest leader. Harbingers' magazine has created this explainer to outline the most important details of the Conservative leadership contest.
Where are the election rules coming from?
Current rules for election of a Conservative Party leader have been approved by the 1922 Committee.
This is a group of Tory backbenchers in parliament, meaning they hold no office in government. It has an eighteen-member executive committee which is voted for by backbenchers and has a chairman who takes charge of key roles of the committee.
They are responsible for orchestrating the leadership contest. Their role includes choosing the threshold for votes in the early rounds and facilitating the parliamentary ballots.
Picture by: michael_swan | flickr
How will the new UK prime minister be elected?
Eligible candidates must have at least twenty MP backers. The 357 Conservative MPs then voted for their chosen candidate on the 13th July, and any candidate who received fewer than thirty votes was eliminated.
Then held another vote on the 14th July and the candidate with the fewest votes was knocked out. This process was repeated on the 18th, 19th, and 20th July until only two remained.
The last election between the final candidates will be open to members of the wider Conservative Party. These members must have joined earlier than the 3rd June, as this date was prior to Boris Johnson's resignation on the 7th June.
The Conservative Party has not revealed the exact number of eligible voters but it should be more than 160,000 people. The voting will be done by either postal ballots or online.
Who are the candidates?
There were originally eight candidates (as of the 12th of July), six of which have already been eliminated leaving only Liz Truss and Rishi Sunak (as of the 11th of August).
Liz Truss has been the minister for women and equalities since 2019, and secretary of state for foreign, commonwealth, and development affairs since 2021.
Rishi Sunak was the chancellor of the exchequer from 2020 before his resignation on the 5th July, following disagreements with Boris Johnson over his approach to the economy.
How will they be campaigning?
The candidates will have the opportunity to gain support at twelve hustings around the country.
These are events attended by members of the conservative party in which the candidates will be questioned about their beliefs and policies.
They take place from the 28th July to the 31st August and are streamed online.
Are young people the biggest losers of the UK Conservative race?
When will the UK have a new prime minister?
The final ballot closes at 17:00 on the 2nd September and the results will be announced on the 5th September.
In the event of one of the candidates dropping out without them being replaced, the remaining candidate will become prime minister and be announced sooner than planned – as there would be no need for a vote.
Theatre reviewer
Brackley, United Kingdom
Born in 2005 in Banbury, United Kingdom, Grace studies at Magdalen College School in Brackley. She plans to study Mathematics at university, and for Harbingers' Magazine she writes theatre reviews.

redpajama_set_name: RedPajamaCommonCrawl


When it comes to buying real estate, particularly the residential sort, choosing one's space can be a perilous task. This is especially true when considering a residence that is half the world away (or just plain not built yet). This is where virtual reality comes in. Thanks to advances in tech, we're seeing more and more buildings and complexes use virtual 360 tours to show prospective buyers just what it's like to walk through their future spaces.
Virtual reality offers for a fresh perspective on custom design by allowing a futuristic display. The unique virtual reality allows buyers to experience the real estate before even being built, or even fully designed. After the virtual reality experience, custom design can be implemented based on buyer preferences, a truly ideal way to create one's dream home. For us Northeastern residents, buying real estate across the country, especially on the West Coast, can be challenging without fitting in a few plane rides. Luckily so many luxury developments are embracing this tech-forward idea of incorporating VR into their marketing strategy. Here are a few leaders in western real estate from California to the Aloha State that are making use of VR in their day-to-day sales.
The Pacific Penthouse Collection, developed by Trumark Urban, and designed by Handel Architects has implemented a full virtual reality build out of one of their penthouse shells. The Penthouse Collection includes four grand penthouses and seven penthouses that can be fully customized in San Francisco, California. Floor plans range from 2,100 square feet to 4,000 square feet. Additionally, multiple penthouses may be purchased for up to 8,000 square feet residence. Designer and contractor recommendations are available. The Pacific is the only new residential development in the Pacific Heights area in several decades. The building itself is located near high-end retail, Michelin-starred restaurants, boasting an exterior which evokes a modern interpretation of classic Victorian aesthetic.
Toscana Country Club, a leader in the virtual reality real estate market, offers upscale, private residential in Indian Wells, California, a town known for hosting some of the country's most prominent tennis tournaments. The residences experience an Italian-influenced architecture and design that range in price from $995,000 to over $4 million. Likewise, Toscana Country Club offers an 18-hole golf course, a performance center, dining facilities, a boutique, and a spa and sports club. Additional amenities to be introduced in fall of 2017 include a casual restaurant, resort-style pool, event lawn, pavilion and two additional tennis courts. Toscana's true appeal is in its commitment to a Tuscan-inspired lifestyle right in the picturesque flats of Indian Wells.
Timbers Kauai – Ocean Club & Residences is a 450-acre resort in Hawaii. When complete, amenities will include a boutique hotel and spa, golf course, and restaurants in addition to a golf course along the Pacific Ocean, shoreline trails, Running Waters Beach, tennis courts, and lagoons. The virtual reality tour offers the Ocean Club and Residencies panoramic tour of Building A at Timbers Kauai and the lagoon-overlooking Building B. The VR experience at Hokuala is perfect for buyers who are interested in purchasing space beyond the West Coast U.S.
The Austin, San Francisco's latest residences, builds on the latest California organic modernism design philosophy and offers tech-focused residences and amenities fit for today's modern professional. This location offers high-tech amenities for mobile professionals such as a Zen-inspired courtyard for relaxation, dog washing facilities, indoor bike storage, and a front desk concierge. Interestingly, the Austin is equipped with conference rooms throughout for an ideal professional environment that transition to dinner tables at night.
Wow, fantastic blog structure! How lengthy have you ever been blogging for? you make blogging look easy. The overall look of your website is excellent, let alone the content!
Hi to all, the contents existing at this site are actually awesome for people knowledge, well, keep up the nice work fellows.


Your employees all carry around, and depend on, many types of devices. Smartphones, Tablets, NetBooks, you name it - they have it. In order to capitalise on this shift in mobile computing, your company needs to put in place a bring your own device policy that protects both your company and your staff. At Attend IT Limited, we have the answer for this necessary integration: Our mobile device management solution.
Enterprise tablet adoption is estimated to grow by approximately 50% per year for the next five years. Clearly larger companies see the benefits of using this mobile technology, but how can small and midsized businesses compete with organisations that can offer these up as a part of their IT budget? By allowing your staff to access your network with their device, your company is able to successfully do what these larger companies are doing, at a fraction of the cost.
Attend IT Limited has a solution for any type of mobile device.
There are many different operating systems that run mobile devices today. To make sure that you are able to facilitate the needs of your BYOD policy, Attend IT Limited provides mobile device management solutions that take this and the variance of service providers into account. We have a central interface that allows administrators to manage devices and data; the core elements of a thorough MDM strategy.
For more information about Attend IT Limited's mobile device management solution, or any of our comprehensive managed IT services, please feel free to call us at 020 8626 4485.


Team Traqmate will be working with Grassroots Motorsports magazine and SafeRacer on a magazine article at the Motorsports Ranch near Fort Worth, TX on Monday and Tuesday, February 25, 26.
Onhand will be pro racers Jason Saini, now with TriPoint Mazda in the SPEED World Challenge series and Andrew Caddell, runnerup in the SCCA Pro Racing MX-5 Cup in 2007.
Look for the article in the magazine sometime before this summer.


Plain Condoms - These condoms are for that Sensual & Close Feeling.
Dotted Condoms - These condoms have specially Moulded dots on the outside to stimulate and heighten the pleasure of love making.
Ribbed Condoms - These condoms have delicately textured ribs designed for extra sensation.
Multi Texture (3 in 1 Condoms ) - These condoms have unique combination of 3 textures to provide extra stimulation for both partners.
Ultra Thin Condoms - Ultra thin condoms are super fine condoms providing greater sensitivity.
Deep Dotted - Deep Dots on the condom for extra stimulation.
Inspiral - These condoms are having spring action for creating that extra pleasure feeling to both partners.
Coloured Condoms - For visual pleasure.


text:
Privacy Policy for Radio Free Fargo
We use Your Personal data to provide and improve the Service. By using the Service, You agree to the collection and use of information in accordance with this Privacy Policy. This Privacy Policy has been created with the help of the Privacy Policy Generator.
Application means the software program provided by the Company downloaded by You on any electronic device, named Radio Free Fargo
Company (referred to as either "the Company", "We", "Us" or "Our" in this Agreement) refers to Radio Free Fargo LLC, 103 North University Drive.
Country refers to: North Dakota, United States
Service refers to the Application.
Information from Third-Party Social Media Services
The Company allows You to create an account and log in to use the Service through the following Third-party Social Media Services:
If You decide to register through or otherwise grant us access to a Third-Party Social Media Service, We may collect Personal data that is already associated with Your Third-Party Social Media Service's account, such as Your name, Your email address, Your activities or Your contact list associated with that account.
You may also have the option of sharing additional information with the Company through Your Third-Party Social Media Service's account. If You choose to provide such information and Personal Data, during registration or otherwise, You are giving the Company permission to use, share, and store it in a manner consistent with this Privacy Policy.
By visiting this page on our website: radiofreefargo.org


text:
Home News Julie almost lands herself in a Ukrainian jail!
Orphan News
Julie almost lands herself in a Ukrainian jail!
Here I am on the airplane from Amsterdam heading home to Atlanta, and I want to write down a little memory of camp before I forget it. It's so hard to think of individual moments because the entire week at Kompas Park was momentous.
I remember it was my team's time to go to the beach. When we got down to the river, I was asked if I knew how to kayak. Sure! Two of the girls, Larissa, and Marina and I, went out in the kayak. Once we got out from shore a little, we noticed these pretty yellow flowers floating in the water. We decided to pick a few. Larissa said, "for Alona" - one of our Ukrainian leaders. So, we really got into it - zig-zagging around the river picking flowers. "There's one" and we were having so much fun — until a boat came up next to us. It was Vasya, the head lifeguard, and he told us we weren't allowed to pick the flowers, as it was illegal! I remembered this as soon as he said it. Oh dear. I felt bad but I couldn't stop laughing because it really was innocent and so much fun trying to pick flowers for Alona.
When we all got back to the landing, I was asked if everything was ok since we were swerving all over the place in the kayak. Apparently, folks on the beach were worried! I know it's really not funny because I'm sure the flowers preserve the eco structure, but it felt like a "Lucille Ball" moment for sure. When I told Cyndee about it, she said she would get me out of jail. But the point is, that it was priceless that the girls wanted to collect flowers for Alona and so it was so much fun doing this together. The girls were so excited about giving flowers to Alona. I'm glad I shared this with them, even if I accidentally broke the law.
Community News Orphan News
There's no place like home… especially for an Orphan
Kompas Park: a magical land of strange costumes, endless mosquito bites, injury-inducing games that you know you shouldn't play but you do anyway, (speaking for a friend...) kids screaming at you for the 100th time to jump on the trampoline, and you have to explain "I'm too old now" in broken Ukrainian, scattered belongings, sunshiny days, tearful goodbyes, and where time moves much faster than normal. There is truly no place like it.
Orphan News Shpola Orphans
Unloved boys do not Grow
Depending on which "official statistics" you believe, there are between 70,000 – 100,000 orphans in Ukraine living in about 750 institutions. This is a huge number. The Ukrainian government is doing its best to reduce it and is in the process of developing a foster care system.
But life is not about numbers; rather the children behind those numbers. Children who need our help. Children like Andrei.
Shpola Grads
Elevate Scholar Update!
How is school going? That's a great question and one that I am asked frequently since I left Shpola orphanage school and started high school at Sophia School as an Elevate Scholar. Well, by nature I am very lazy, and I got used to doing very little schoolwork at the orphan school. Even though I was the top pupil in my class, I realize now that I have huge gaps in my education and there are many subjects I need to catch up on. Now, I am a senior at Sophia School, and even though the dear principal Natalia tries to motivate me, I have not had much success. So, I decided that I can't do this on my own, and I prayed about it and today I received an answer.
Sign up to receive regular email news updates and volunteer opportunities in Ukraine
Who cares about Care Givers?
Motherhood is bittersweet
A piece of my heart remains in Ukraine
Charlie comes back for more at Kompas Park!
Remembering God's Perfect Love in a World of Broken Love
What are You Doing this Summer?
Christian Journeys
Shpola Orphans
Sophia School
Hope Now Ministries
2090 Dunwoody Club Drive
info@hopenowusa.org
    ✉
© Copyright 2020 Hope Now Ministries. All rights reserved.
Stay Informed about our work
Hope Now Ministries is a 501(c)(3) tax-exempt charity.
Federal Tax ID# 75-2067420.
All contributions designated for specific projects shall be applied to those projects.
Occasionally we receive more contributions for a given project that can be wisely applied to that project. When that happens, we use these funds to meet an urgent need.


Finding homes for sale in Old Palo Alto can be quite difficult, for one reason: once residents move in, they never want to leave! The vast majority of the homes are owner-occupied and residents range from young professionals raising new families to elderly couples. Old Palo Alto's greatest asset is its pedestrian-friendly atmosphere, with tree-shaded streets and homes that are all singularly elegant.


text:
Mom sues when son doesn't make varsity team
The mother of an unnamed Ladue junior who did not make the varsity cut does not think that's fair. She believes her son, who already played on the JV team, is good enough play JV again.
By | October 1, 2018 at 9:09 AM CDT - Updated October 1 at 9:22 AM


Bethany Beach is in between OC and Rehoboth, but far enough away that it's quiet. Actually, there's a nature preserve in between Bethany and OC, so the noisiest thing we experienced after getting there was the road.
Overall, the setting and the company were pleasing. Although we were there via the invitation of our friends Ian and his wife Alida, the house was also shared with three single mothers and their assorted collection of 6 children. As the weather worsened, cabin fever set in, and the children became less and less bearable. We all survived (including the children) but for a time it was close.
My normal medical difficulties did not present themselves; instead, other difficulties loomed. For example, the climate control in the shul where we prayed was also defective, and I was nearly overcome with heatstroke. I returned to our place and slept most of Friday away, recovering from my exhaustion...leading some others to lay a diagnosis of a sleep disorder on me. Yay.
All in all, it was a good trip. Prayer was performed. Food (especially milk products) were consumed. And Ian introduced me to Johnny Walker Green. Mmm...green. I truly had a good time, and we think we will do it again next year, despite the weather and occasional difficulties with the company.
We came back via a different route (we avoided 404) and to my scheduled shift without significant difficulty. I was pleased to see that although there had been computer problems while I was gone, that the problems had been resolved in my absence. And there was much rejoycing. Next time, however, I'm taking Sunday off so that we don't have to rush back.


text:
Q: Remove font using iText I have a problem with my PDFs. They have a default font, Helvetica. This font is unused, but I need to develop a script that automatically deletes this font.
After asking two questions:
Unset PDF font with script
xhtml2pdf doesn't embed Helvetica
I finally discover iText. I've been trying to work with this library, and I made a few successful tests with Unembeding Fonts (example). But I can't find anything about deleting a whole font from de PDF.
Thanks a lot

redpajama_set_name: RedPajamaStackExchange


BLOG - Katie Jade Portfolio - Help Me Choose A Winner!!
Help Me Choose A Winner!!
The photographs you shared with me for the #KatieJadePhotoChallenge were fantastic! I'm so proud of everyone of you who took part. Each photograph was unique and thought provoking, which shows you all really thought about the themes very creatively.
Changes - I saw a lot of personal changes, whether it would be about a person and their body or outlook, changes in their environment or steps people are taking to make changes. It was a beautiful collection to start the challenge!
Character - This theme was taken in two directions. The first was a human characteristic or fictional character. The second was a characteristic of furniture and architecture. There was a really nice mix of the two.
Temptation - This theme was a lot of fun. A lot of people looked at giving in to food or buying something they wanted. Others looked at the negativity of temptation, reflecting weaknesses in humans. The difference in the type of imagery was quite dramatic!
Light - I really liked seeing the photos for this week. Light could take on so many forms, the images I saw were so varied. There was some of different types of lighting (bulbs, fairylights, etc). Some people looked at how light can transform natural surroundings. Others looked at the word "light" and what it meant to them. Another week of fantastic photography!
In To The Future - This was a tricky theme, I admit, but I really wanted to get you thinking creatively… and you did! It was nice to see a lot of you thinking about your own future and where you would like to see yourself. There was a lot of positive imagery which was a fantastic way to end the challenge.
So… who won? Well that's the thing, I have found it so difficult to decide on a winner. I looked through and pulled out my favourite photo from each week, and I thought to myself "Everyone has spent so much time and effort to take part in this challenge, so why don't I make the choosing of the winner even more exciting, fantastic and nail-biting… hmmm"… So that is what I have done.
Voting closes at 23:59pm on the 9th September 2015.


text:
70-year-old tortoise recovering after California man charged with animal abuse
By Azenith Smith
Man arrested after tortoise abused
A San Jose man is facing felony animal abuse and vandalism charges after police said he broke into a preschool and badly abused the school's pet tortoise. He's named Michelangelo after the character from the Teenage Mutant Ninja Turtles. Azenith Smith reports
SAN JOSE, Calif. - A San Jose man is facing felony animal abuse and vandalism charges after police said he broke into a preschool and badly abused the school's pet tortoise.
Michelangelo, the 65-pound African sulcata tortoise named after the character from the Teenage Mutant Ninja Turtles, is estimated to be 70 years old. He is not moving much these days, recovering at a pet hospital from a horrific attack.
San Jose man arrested for animal abuse, vandalism
"It was a horrific sight," said Tammy Lariz, the administrator at Play 'N' Learn Preschool. "The beating must have been bad. There were several items they used to beat him with. Knowing his shell is meant to withstand a lion's attack in Africa you know he had to be hitting that tortoise really hard."
Around 11:30 a.m. Saturday, police said 40-year-old George Robles, listed as unhoused in San Jose, broke into the preschool and beat Michelangelo.
George Robles mugshot. Photo: San Jose police dept.
Police said Michelangelo was found with a piece of wood stabbed through his outer shell.
The school's administrator fears Michelangelo would have bled to death had a neighbor not called the police.
"It was pretty brutal," said Dr. Tal Solomon of Archvet Animal Hospital. "The first thing I thought of was it takes such a special person to cause such harm to an animal that can't even get away."
Archvet treated Michelangelo right away. Solomon called it the worst case of abuse he's seen. Michelangelo underwent surgery to repair his shell and to remove the wood and glass shards. The doctor used a special apparatus to help raise his shell.
None of Michelangelo's organs were damaged. The hospital is doing everything for free.
"In my book, there's no option," said Solomon. "He needed help. She couldn't afford it so you end up doing the right thing."
The preschool took the tortoise in four years ago from a rescue group. The kids have taken turns riding on his back.
The pet hospital plans to keep Michelangelo for now and hopes he'll be back at the school in two months.
"We will all be super excited when he's fully healed," said Lariz.
A lot of people want to help with Michelangelo's recovery so the pet hospital is taking donations to help other abused or hurt animals. Venmo username: @archvet1.
The suspect is undergoing a mental health evaluation.
As for how to tell the kids, the preschool plans to talk to them at their level tell them their pet is at the hospital and he's getting a bandage.
Online petition demands release of Texas brothers accused of killing stepfather over alleged abuse of sister


text:
Returning to campus: January 2021
UCB Portal
Courses starting in March 2021
A-Z of all courses
Online and part-time
Not sure what course is right for you? Try our course finder
Experience what it's like to study at University College Birmingham
Health and safety on campus
Living in Birmingham
UCBloggers
University of Birmingham accreditation
Our governance and management
Academic staff directory
Meet and greet service
International tuition fees
International UCBloggers
hired@UCB – careers, employability and placement support
Helping you start your business
Goosefoot scoop top prize for best vending snack
Share post: Share on Facebook Tweet
Read time: approx 2 mins
Final-year Culinary Arts Management students staged an exhibition of healthy vending snacks for the Automatic Vending Association's (AVA) Culinary Product Development Challenge.
Working in teams, the UCB students were tasked with developing a delicious new range of products to meet calls for healthier vending alternatives. The results were then judged by representatives from across the food, health and vending sectors.
The judging panel, which included representatives from Unilever, Mondelez and the NHS, selected Goosefoot because of its strong understanding of the product and how it would fit into the vending industry's business sector.
The judges said Goosefoot showed "absolute passion" for their product, which met all of the target criteria of being a vending snack product that was nutritionally-balanced, tasty, well thought-out and under 250 calories.
Goosefoot's winning concept consisted of two quinoa bars self-contained in carbon-friendly packaging with one bar being sweet and one savoury. The team was made up of six students: Simon Whitby, Amelia Pratten, James Wood, Chris Luscombe, Michael Chatwin and Gavin Youngson. They developed their idea for a quinoa bar after spotting a gap in the vending market for a healthy snack bar that was free from allergens and artificial ingredients.
The other five teams were Big Momma's Kitchen, Super Pops, Synergy, Fill Me Up Cups and Posh Nosh.
Bernhard Schumacher, Module Leader for Culinary Arts Management at UCB, said: "We are extremely proud of all the teams. The creativity, culinary talent and professionalism they showed for the project was amazing. The students made their learning visible by developing a variety of healthier product concepts for the vending industry. The level of teamwork and attention to detail was incredible."
Tim Franklyn Hays, head of the culinary service team at Unilever and a judge, said: "It was evident that the students had worked very hard on their projects over the last few weeks. They had clearly taken on board the feedback was given to them at the mid-point of the project and the sheer amount of enthusiasm for their projects during the final presentations was very impressive. These guys really believed in their products and the presentation skills were brilliant and well-practised. All the students are a credit to UCB and their future industry. I look forward to being involved in this project next year."
Jonathan Hilder, CEO of the AVA, concluded: "As well as being trialled across Bristol NHS Trust, the winning team will join the rest of the vending industry at our biennial event, AVEX International 2015, next June where they will have a stand in the health and well-being area and show their product and also showcase the University.
"We are excited by the results of this year's challenge, and what we hope will be the start of a new generation of healthier products available through vending."
College term dates
Postgraduate term dates
Information for parents (College students)
Access the UCB Portal
Data protection resources
Higher education student handbook
College student handbook
Disability statement
© Copyright 2021 University College Birmingham. All rights reserved.


In March 2010 the Box Hill Hawks Football Club announced the first 48 official "Locker Legends" of the Club. In 2011 a further four players were recognised as "Locker Legends".
These players have played over 100 Senior games for the Club, been selected in B.H.F.C.'s "Team of the Century" announced in 2000 or been drafted from the Club and played at least one A.F.L. game.
Lockers in the players' rooms at Box Hill City Oval commemorate the names and achievements of these Club champions on the locker carrying the number of their playing jumper.
To date 14 players have been welcomed back to the Box Hill Hawks Football Club to be officially inducted as 'Locker Legends'.


Dewalt DWS780 Double Bevel Sliding Compound Miter Saw, 120 V, 15 A.
One of the most handy inventions for woodworkers, miter saws are a must have for most DIYers. At PlumbersStock, you can save on all the best power tools and that includes the miter saw (also known as chop saws). Buy one and cut wood at any angle you need or browse our other power saws.
Get great deals on all your tools online, whether you need miter saw parts or any number of products from the most trusted manufacturers: Milwaukee, Ridgid, and Greenlee.
Miter saws are mechanically powered circular saws that offer accurate cuts on-the-go. From miter and bevel cuts to compound cuts and crosscuts, miter saws are the perfect tools to tackle new home improvement projects head on.
Operating with a blade mounted on a swing arm that pivots left or right, these saws produce angled cuts that be used for applications such as cutting crown molding, picture frames, door frames, and window casings. There are three types of miter saws: basic, compound (dual or single), and sliding compound.
Basic Miter Saws – These kinds of saws are used for crosscuts and miter cuts. They have no bevel option but have the benefit of working easier, faster, and more accurately than using manual miter boxes and a handsaw. Combined with a low price tag and models that provide a more comfortable operation, this saw is a must-have for performing simple home improvement tasks.
Compound Miter Saw – There are two options in this category, single compound and dual compound. While both single and dual compound miter saws are used for cross-cuts, miter cuts, bevel cuts, and compound cuts (miter + bevel), only dual compound saws can tilt in two directions. This allows the user to quickly create bevels at any angle without having to flip your workpiece.
Sliding Compound Miter Saw – These saws create the same types of cuts as regular compound miter saws but offer greater versatility. With help from the sliding head, this saw allows the user to cut through wider pieces by allowing you to move the blade forward and backward. This provides the increased length of cut that is necessary in so many projects.
Miter saws come with many optional features to assist you in your project. When shopping for the right miter saw, it's important to keep these features in mind for enhanced efficiency and ease-of-use.
Wide Miter Range – Most miter saws offer maximum cuts that range from 50" to more than 60". If your project requires long cuts, check the specifications and ensure your saw offers a wide enough miter range.
Sliding Fence – A sliding fence provides full-height support for miter cuts and slides out of the way for bevel cuts.
Adjustable Handle – Some models offer multiple handle positions that you can adjust to give your grip maximum comfort and control.
Laser Guide – Some models feature laser guides that help you line your blade up with the intended cut line. Saws with a single laser show where the cut starts, while saws with dual lasers show the entire width of the cut.
Dust Port – Dust ports let you connect a dust bag to your saw so you can collect sawdust as you work. This helps when you're cleaning up your workspace after you're done and allows you to maintain a clear environment.
Electric Brake – A model with an electric brake heightens safety by stopping the blade automatically as soon as you release the trigger.
Easy-Change Blade – Some models offer one-step blade changes that enhance the user's safety. If you plan on swapping out blades frequently, choosing a saw that makes the process easy and quick can decrease downtime to let you get back to work faster.
Stands – Stands can be purchased to give your miter saw better portability. If you cut long stock, some stands provide extensions to support longer work pieces.
Save on the best saws available anywhere online, when you buy miter saws wholesale at PlumbersStock. If you have any trouble locating the right tool, please contact our customer service team.


text:
TODAY ON VOICES: Help Us Build Voices, US Out of Iran, The Irishmen, Just Mercy and Goop!
Dear Voice Listeners
Thank you for supporting our program for more than 15 years. Like you we always want a New Year's focus and innovation. This year our primary objective is to build a Voices from the Frontlines Organizing Community. What we mean by that is to keep expanding "listener movement radio" more than "listener sponsored radio."
1) Please write us early and often about feedback for the show, suggestions for future programming, books and films you want reviewed or publicized. I always ask during the show, "please email us at eric@voicesfromthefrontlines.com Many of you do and that is great. We also are working on a volunteer to help me make sure I respond to the emails I do receive. I will try to acknowledge each email on the following show and read from some of them.
2) Please support the causes that we highlight on the show. When I say, "this group is doing great work, please contact them at their website, it means a lot if groups get back to me to say, "I got 10 contributions, 5 people volunteered, 15 signed up for our email list.
3) Can you get 5 friends to become Voices listeners. First, by signing up on our website–VoicesfromtheFrontlines.com so they get our weekly email letter. Then remember, you can listen to our show at any time–we love you from 3 to 4 every Tuesday but thanks to our website and our podcast Voices is on 24/7.
4) Help us build our podcast I still have ideas about doing a shorter 15 or 20 minute podcast but the show itself is a podcast so you can download the show any time on Apple Podcasts, TuneIn, SoundCloud, Stitcher, and Google Play
5) Please like us and follow us on Facebook & Twitter: @EricMannSpeaks and help us build our audience on Instagram: @voicesfromthefrontlines. Help us get the word out. I will talk about this more today on the show and every show it will be an actual part of the programming–how to build The Voices From the Frontlines organizing community.
Today on Voices from the Frontlines:
U.S. hands off Iran. Reparations to the people of Iran
January 7, 2020 @3pm PST
U.S. hands off Iran. Reparations to the people of Iran for the murder of Iranian General Qassem Soleimani by president Donald Trump.
This latest of so many murders and assassinations is part of the long traditions of the barbarism of U.S. assassinations of political opponents. This Democratic and Republican tactic, raised to a horrible crescendo by President Barack Obama, is rooted in the U.S. imperialists' effective ideology that the U.S. is always under attack. This in turn is deeply rooted in the mindset of the imperialist white settler state and the white settlers themselves. Sadly this ideology and consciousness is now widespread among people of all races inside the U.S. imperialist white settler state. How do we build a real movement against the U.S. imperialist white settler state, its ideology, and its deep roots inside all of us! Reparations to the people of Iran. No drone attacks. UN Sanctions against the U.S. No U.S. attacks on Iran, Iraq, Afghanistan and the world.
Consumer Advice Column: Gwyneth Paltrow's : "luxury lifestye" Goop give's vacuous careerists a bad name.
Short Film Review: Martin Scorcese's The Irishman—a worthless piece of garbage!
Film Review: Just Mercy: about the life of civil rights attorney Brian Stevenson, a wonderful film about courage, the racism of U.S. society, the cruel and unusual punishment of the death penalty, and the will to fight on. A MUST SEE for everyone. Great thanks to Black Lives Matter, many community groups, Warner Brothers, and the Cinemark Theater in the Crenshaw Mall (one block away from our Strategy and Soul Movement Center 3546 Martin Luther King Jr Blvd at Crenshaw. Jamie Foxx was profoundly moving as death row prisoner Walter McMillan, Michael B. Jordan, very impressive in an understated Stevenson, Brie Larson as Eva Ansley, the type of anti-racist white southerner there used to be more of and a credit to her race (credits to Damu Smith for that line) and Tim Blake Nelson as white prisoner Ralph Myers on whose turnaround to hopefully do the right thing so much of the outcome is based. If the Battle of Algiers is the only 100 I'll ever give a film and 0 is the rating for The Irishman than Just Mercy is beyond any number, a very fine film that every voices listener should become a viewer.
Listen to Voice from the Frontlines today at 3pm PST on KPFK 90.7fm
OR use the button below to Stream the show live on KPFK.org


798 is changing, so much of it was missing from my 2012 trip that it was very sad to see what is happening to what is singlehandedly the most amazing place in China if you love modern art. In so many ways Beijing can be a crowded sterile city with small pockets of live amidst the high rise buildings. 798 defies everything you thought about china from the time you walk in the area, to the time you leave every sense from touch to taste (awesome food and drinks) to visual art to everything that surrounds you invades your mind and you will leave changed. 798 is a unique place in Beijing, there might be other places like it, but 798 is a legend.
The interesting part is what you can find there. Everything from all art forms are there. The wall art is the most special and amazing to me. It is simply world class, and would not be surprised to find a Banksy or other world class street artist working out of the spaces there. The other interesting part is that there is art there that can be found on the streets of Seattle. When you see a slap tag on a wall in 798 on a USPS priority mail label, you know that there is communication and sharing between Chinese slap taggers and American slap taggers. I almost fell over to see something like that, and would love to know more about how they communicate and how they share stuff happens. I would love to see some Chinese slap tags floating around Seattle.
It does look like the area is subcuming to the pressures of urbanization. Significant portions of 798 were abandoned this year. Pressure of high rises and other mass housing are everywhere in the area, and that has to be putting pressure on the galleries and artists that live and work there. I have no idea where they will go once 798 is gone, but I hope to find it when they do finish moving to their new digs. China faces incredible population pressures that Americans can only guess at. In a land that is 5000 years old, in a culture that has seen a little bit of everything, 798 manages to stand out as unique. It would be a shame if the area was eventually bulldozed for more high rise housing that few people can honestly afford. (One of my Chinese friends was telling me that it is 50,000 RMB per square meter of an apartment in a high rise, and you only own it for 50 years not forever. $8,000 roughly for those who want to do a quick conversion in your head).
798 deserves to live, but that is up to the Chinese government, and the artists that need to be there to help the community thrive. In all though, for as long as it exists, this is a vibrant and amazing place to go visit, you should soon, because it might not be there for very much longer.


We're going back to the basics. Watch this short, easy-to-digest, and actionable 15-minute on-demand webinar to learn the fundamentals of digital marketing. We'll define the acronyms, translate the buzzwords, and give you the foundation you need to understand and implement an effective digital marketing strategy.


text:
New Revised Standard Version Updated Edition (NRSVUE)
New Revised Standard Version Updated Edition
The Command to Sacrifice Isaac
22 After these things God tested Abraham. He said to him, "Abraham!" And he said, "Here I am." 2 He said, "Take your son, your only son Isaac, whom you love, and go to the land of Moriah and offer him there as a burnt offering on one of the mountains that I shall show you."(A) 3 So Abraham rose early in the morning, saddled his donkey, and took two of his young men with him and his son Isaac; he cut the wood for the burnt offering and set out and went to the place in the distance that God had shown him. 4 On the third day Abraham looked up and saw the place far away. 5 Then Abraham said to his young men, "Stay here with the donkey; the boy and I will go over there; we will worship, and then we will come back to you." 6 Abraham took the wood of the burnt offering and laid it on his son Isaac, and he himself carried the fire and the knife. And the two of them walked on together.(B) 7 Isaac said to his father Abraham, "Father!" And he said, "Here I am, my son." He said, "The fire and the wood are here, but where is the lamb for a burnt offering?"(C) 8 Abraham said, "God himself will provide the lamb for a burnt offering, my son." And the two of them walked on together.
9 When they came to the place that God had shown him, Abraham built an altar there and laid the wood in order. He bound his son Isaac and laid him on the altar on top of the wood.(D) 10 Then Abraham reached out his hand and took the knife to kill[a] his son. 11 But the angel of the Lord called to him from heaven and said, "Abraham, Abraham!" And he said, "Here I am." 12 He said, "Do not lay your hand on the boy or do anything to him, for now I know that you fear God, since you have not withheld your son, your only son, from me."(E) 13 And Abraham looked up and saw a ram, caught in a thicket by its horns. Abraham went and took the ram and offered it up as a burnt offering instead of his son. 14 So Abraham called that place "The Lord will provide,"[b] as it is said to this day, "On the mount of the Lord it shall be provided."[c]
22.10 Or to slaughter
22.14 Or will see; Heb traditionally transliterated Jehovah Jireh
22.14 Or he shall be seen
22.2 : 2 Chr 3.1; Heb 11.17
22.6 : Jn 19.17
22.7 : Jn 1.29, 36; Rev 13.8
22.9 : Heb 11.17–19
22.12 : Gen 26.5; 1 Sam 15.22
New Revised Standard Version, Updated Edition. Copyright © 2021 National Council of Churches of Christ in the United States of America. Used by permission. All rights reserved worldwide.


text:
Ice hockey goalkeeper Martijn Oosterwijk from Groningen wants to promote with Orange. "We belong to this level"
9 months ago Richard Powell
Martijn Oosterwijk in front of Martinitoren Photo: Siese Veenstra
Goalie Martijn Oosterwijk (31) of Groningen starts his seventh Ice Hockey World Cup in Croatia on Monday. "I think we belong to a higher level."
Hi Martin, how are you already on the way?
,,Welcome! Yes, on the way, but not yet to Croatia. I am now driving from Groningen to Tilburg for the last training session with the Dutch team. I'm going 500 more miles today. We look forward to it. Especially because of course no international matches have been played in the last two years. "
She will play in Division 2-A next week against Spain, China, Croatia and Israel. Not exactly the best countries in ice hockey, I think?
No, not that, ha ha. The Netherlands is now playing the fourth tier. When we get promoted, we play Division IB. Above that are Division I and Division I, which includes major countries such as Canada, Finland, and the United States. Of course we can't match it, but Division IB, which we've played in before, should really be possible. I think we belong to that level."
What are your chances of promotion?
It's still a bit hard to appreciate, because international ice hockey has also been silent for about two years. We have to win the group, then we are promoted. I think China will be the strongest opponent. This team also played as the host country at the Winter Olympics of course. After that comes Croatia and then Spain and Israel, I think."
See also Ukraine sends a full delegation to the 2022 Winter Paralympics
With UNIS Flyers Heerenveen, I recently won the BeNe-League, the Netherlands and Belgium Championships, after the national championships went to HIJS Hokij. Do you still have a good year?
"Yes, you can say that. We are especially happy that everything has started again. Nice that it is possible again, for a full stadium to play ice hockey. I am also very excited about it."
Are you a professional athlete as an international ice hockey player?
"No, if only that were true. I completed my studies in sports management last year and recently started working in a watch and jewelry store on the city's Stoeldraaierstraat. That's fine."
Richard Powell
Tv fanatic. Freelance thinker. Social media enthusiast. Total bacon lover. Communicator.
Previous Bo Bendsneyder starts fifth at the Indonesian Grand Prix, Zonta van den Goorbergh 25 | other sports
Next Bo Bendsneyder starts fifth at the Indonesian Grand Prix, Zonta van den Goorbergh 25 | other sports
Lionel Messi's Argentina career
Porsche explains why the deal with Red Bull fell through, "The RB19 will be the evolution of the RB18" | GP fan summary
1 month ago Richard Powell
Cruciate ligament rupture, participation in the World Cup is very unlikely
Bridger Joris van Lankveld: One card difference and we won't win the European Championship
The PSG president believes that Messi and Mbappe will remain at the club


Integral View provides coaching, presentations and education programmes to executives and organisations that are seeking insight and inspiration to meet the complex challenges of our time.
Personally, as organisations and as whole societies, we are finding ourselves 'in over our heads' in situations that are calling for psychological growth towards more sustainable behaviour – but the spreading of awareness of how this internal process operates is still in its infancy. Over the last few years, leading research from developmental psychologists at such places as Harvard University has started to synthesise into a greater understanding of how this process takes place and how it can be facilitated – called Integral Theory. This has been the basis for such groundbreaking works as Frederic Laloux's 'Reinventing Organisations'.
Working with this understanding brings deep insight to guide more efficient action towards developing a sustainable world. Our contact is Jonathan Dover who works as an Integral Leadership Coach and Consultant.


text:
UofSC ranks No. 1 in online graduate nursing program
College of Nursing has two Top 10 rankings in latest U.S. News report
Posted on: January 24, 2022; Updated on: January 24, 2022
By Megan Sexton, msexton@mailbox.sc.edu, 803-777-1421
The University of South Carolina's College of Nursing retained the No. 1 national ranking for its online graduate nursing program, according to U.S. News and World Report's annual online program rankings released Tuesday (Jan. 25).
The university's online master's in nursing administration broke into the Top 10, moving up five spaces from the previous year and now ranks as the nation's 9th best. The nursing school also kept its No. 1 spot for the second consecutive year as the top online nursing graduate program for veterans.
Online graduate programs are particularly important in nursing because they offer flexibility to the many students who already are practicing in the field. UofSC's success in this area demonstrates both its academic excellence and its commitment to providing flexible degree pathways.
"The College of Nursing continues with its steadfast momentum of excellence in the preparation of nursing professionals to advance their careers," Dean Jeannette Andrews says. "Working nurses, now more than ever, need flexibility and convenient alternatives to traditional classroom instruction, with expectations for rigor and highest standards expected for graduate education. The online 'classroom' — along with clinical rotations and other experiential learning in their own regional health systems — provides graduate nursing students an opportunity to continue to advance their education, which otherwise may be limited."
The College of Nursing has placed in the top 10 of the online graduate rankings for the past eight years. The college also is in the top 1 percent in the country for pass rates on the NCLEX, the national exam for licensing nurses.
"Our world-class faculty and student community service during the pandemic has been extraordinary, yet not unexpected, as they are trained to advocate on behalf of their patients and communities. Our team truly 'walks the talk' to keep the public healthy and safe and are exemplary role models and leaders," Andrews says.
Other UofSC highlights in the U.S. News rankings include:
The College of Education improved to No. 20 in the best online graduate education programs, moving up three spots from the previous year.
USC Aiken moved up 15 spots to No. 87 in the best online bachelor's programs, and the campus was also ranked among the best online MBA for veterans (No. 90).
"As the state's flagship university, we continue to prioritize accessibility, so that any student who wants to earn a college degree has a pathway to do so," Interim President Harris Pastides says. "I'm proud to see our top-ranked online nursing programs recognized for providing high-quality academic experiences and preparing the health care professionals we need in South Carolina."
Topics: Faculty, University Leadership, University President, Academics, Graduate Studies, Recognition, Health Sciences, Careers, College of Education, College of Nursing, University System


text:
De bende van de Witte Veer is een Nederlands jeugdboek uit 2017 dat geschreven is door Paul van Loon en geïllustreerd is door Hugo van Look. Het boek is een vervolg op Raveleijn.

Achtergrond
De bende van de Witte Veer was van origine een roversbende die zich in de 17e eeuw vestigde in Kaatsheuvel en omstreken. De bende maakte dankbaar gebruik van de grens tussen Holland en Brabant om vervolging te ontlopen. Aan beide zijden van de grens van Staats-Brabant en Holland gold een andere jurisdictie, waardoor ze minder gemakkelijk gepakt konden worden.

Nederlands kinderboek
Werk van Paul van Loon

redpajama_set_name: RedPajamaWikipedia


This recipe can be made with ground beef or chicken instead of turkey, but this is our take on eating a little healthier. If you can't find a chipotle in adobo sauce, feel free to just use a jalapeño and chop it up and add a little barbecue sauce to compensate for the lost adobo.
assembled and placed back in the fridge it ensures the burgers stay together better.
large bowl. Use your hands to mix well and form into 8 patties.
Preheat either your Silverbac, Kong, or Grilla to 375 degrees.
patties, you must cook them until they reach 165 to avoid any issues.
Just before the patties are removed from the grill, top with the cheese and serve.
n Bold is a perfect pairing with these burgers as well.


Treat yourself to a light but luxuriously rich body lotion that helps nourish itchy, dry skin and maintain softness. Best-selling formula absorbs quickly without feeling greasy or sticky and is made with nutrient-rich Dead Sea water, aloe vera and smoothing Witch Hazel. Replenish skin with 21 essential minerals, including magnesium, calcium, and potassium. Unique aromatherapetic scent featuring pineapple and white peach. Formulated for daily use to elevate and lock in skin's moisture.


text:
Building a Corporate Culture with Play at the Core
By Elizabeth Cushing
FOLLOW :: Human Resources | Leadership | Management
Seminar: Developing Your Emotional Intelligence
Seminar: Building Better Work Relationships: New Techniques for Results-Oriented Communication
Seminar: Conflict Management Workshop
Organizational culture is the sum of the values and behaviors that contribute to the unique psychological and social environment of an organization. Play permeates our culture at Playworks, which I believe has helped us navigate challenges and opportunities over the past 22 years.
Our organization partners with elementary schools to build healthy school cultures through play, and this year we will reach 1.25 million kids through our efforts. Researchers have closely evaluated our impact and play's extraordinary ability to bring out the best in kids. Less visible is how play brings out the best in our staff.
The same programming that we bring to kids—the emphasis on play as a tool for promoting trust and rapport—is an important part of how the Playworks staff interacts. Getting out from behind our screens to play together helps us practice greater empathy, better understand our peers, and sharpen our social and emotional skills, such as conflict resolution and collaboration. That's exactly what play does for children.
"It Pays to Play," a report by BrightHR, explores the benefits of corporate play in a three-generation workforce and how having fun trickles down to productivity. The study revealed that playing at work not only motivates individuals as they feel a greater sense of purpose in their companies but also strengthens skills and teamwork, decreases stress, positively transforms office morale, and creates a baseline of emotional safety, all of which collectively helps to drive efficiency.
Corporate social responsibility is shifting
The 21st-century civil rights movement is changing corporate social responsibility. Not only are companies taking a stand on societal issues, such as climate change, sexual harassment, gender pay equality, and gun violence, but they're now refocusing on the health and well-being of the heart and soul of their brands—their employees.
Studies show that employees who feel valued are more productive and work better together. And companies that authentically support their employees' professional, social, and emotional needs have higher retention rates. According to a recent survey Playworks commissioned on behalf of US Bank with adults not affiliated with either brand, 98% of participants think play is valuable for adults, as play increases physical activity, brings people together, gives individuals a brain break, and fosters creativity. The survey revealed that nearly two in five Americans say they would ideally like to participate in active play at least once a day, but 38% report financial stress prevents them from engaging in playful activities.
At Playworks, we walk our talk. As the president, I am aware that how I show up to play has an enormous impact on how I am received as a leader. Our staff watches how I pay attention, how I work with our teams, and how I handle mistakes. Through playing, our leadership is able to articulate and model our organization's expectations, philosophies, and values.
Playing in the workplace
We start every meeting with a random question. Check-in questions—such as "What was the first concert you attended?" or "What celebrity would you be friends with and why?"—remind us of our shared humanity and the three-dimensional nature of our lives. They give individuals opportunities to practice sharing and presenting to teams. Throughout meetings, we facilitate playful improvisation activities to foster collaboration and creativity. This enhances the brainstorming process and ultimately allows us to problem solve, develop, and test ideas in a safe, healthy, and engaging way.
Our Corporate Playbook offers examples of office games in four categories:
Icebreakers are effective and fun ways to quickly build community among co-workers (example: check-in questions)
Minute moves and energizers are short, simple games that energize the office (examples: Indoor Marco Polo and Hot Potato)
Cooperative indoor games provide great opportunities for team building and having fun (examples: Tic-Tac-Toe Toss and Charades Relay)
Cooperative outdoor games allow staff to get some fresh air and take a work break (examples: Sardines and Relay Lock Race)
Remember that playing must be safe and engaging for all. Modify games so that everyone feels welcome to participate. Leverage competition as a healthy way to reinforce working toward shared goals and encourage others not to give up and to do their best, win or lose. Doing so not only enhances your organization but will be a lot of fun in the process.
As a collaborative leader, you can break down silos, energize your team, and inspire optimal performance.
Elizabeth Cushing is the president of Playworks, the leading national nonprofit leveraging the power of play to transform children's social and emotional health.


Wizkid ft Teni Pansa Mp3 Download. Starboy Wizkid took to his Instagram live to tease his fans with snippet of his forthcoming track called Pansa which he had with the DDE song bird Teni.
Fans has been expecting this tune since Wizkid teases it. Pansa is also said to be a take off track from his soon to be released produced Made In Lagos EP which features other top Nigerian singers.


text:
/ Electronic Components
Cleartronic, Inc. (CLRI) Breaks 40 Million in Radio Transmissions as Both Customer Base and Transmissions Continue Rapid Growth
(businesspress24) - BOCA RATON, FL -- (Marketwired) -- 04/27/15 -- Cleartronic, Inc. (OTCQB: CLRI) announced today that ReadyOp™ users have now sent over 40 million radio transmissions via the . Cleartronic, through its subsidiaries, is the owner of patented radio over IP technology enabling true interoperability between radio talk groups regardless of frequency, manufacturer, location or device, including laptops, personal computers and smartphones using the ReadyOp DT application.
"With our installed base of users in the United States and internationally, we now are seeing the radio traffic exceeding 100,000 transmissions daily," said Marc Moore, a director of Cleartronic, Inc. and Inc., a wholly owned subsidiary of Cleartronic. "This is solid proof of the strength and reliability of our ReadyOp communications platform and our patented radio to IP gateways. We are also seeing increased daily use of our ReadyOp DT smartphone application, especially by first responders and emergency operations personnel."
"We are pleased at the continuing growth and use of ReadyOp by our clients. The 40 million milestone for radio communications, plus the monthly growth in total text messages, alerts, emails and other voice communications provided by ReadyOp, shows that ReadyOp has become a integral part of our clients'' daily operations as well as their incident and emergency response plans," .
ReadyOp is a secure, web-based platform providing organizations with a single site for planning, response, communications and documentation of personnel, tasks, assets and activities. ReadyOp is in use by federal, state and local government agencies, hospitals, schools and universities, ports and airports as well as corporations. ReadyOp provides simple, flexible communications capabilities, including true interoperability for radio talk groups for personnel in multiple organizations. ReadyOp supports daily operations and special event planning plus incident management, emergency response, recovery and continuity of operations (COOP).
About Cleartronic, Inc.
(OTCQB: CLRI) is a technology holding company that creates and acquires operating subsidiaries to develop, manufacture and sell products, services and integrated systems to government agencies and business enterprises. ReadyOp Communications, Inc. is a wholly owned subsidiary of Cleartronic,Inc. -- .
This press release may include predictions, estimates, opinions or statements that might be considered "forward-looking" under the provisions of the Private Securities Litigation Reform Act of 1995. Such statements generally can be identified by phrases such as the Company or its management "believes," "expects," "anticipates," "foresees," "forecasts," "estimates," or other, similar words or phrases.
American Media Group, Inc.
3032 East Commercial Blvd., Suite 18
clri, 911, emergency-call, police, fire-department, ambulance, first-responders, public-saftey, florida, sos,
Firma: Cleartronic
Stadt: BOCA RATON, FL
More PressReleases in Electronic Components
Isabellenhütte receives type examination certification from PTB Braunschweig
As a specialist for shunt measurement technology, Isabellenh the designs of their applications. The basis of the type examination certification was the successfully conducted metrological measurement series (accuracy class B), the operation of the softwa ...
iDTRONIC's RFID BLUEBOX Micro IA | Cylindrical Reader M30: Optimized Demo Software Features for an even more effective Application
► AUTOMATIC READOUT OF MEMORY UNITS ON TRANSPONDERS The automatic readout of memory banks on transponders or tags is now possible . Two different speeds can be selected for continuous recording. The data is sent as BLUEBOX telegram or ASCII form ...
UltraSoC furthers academic support with Europractice partnership
CAMBRIDGE, UK – 4 November 2019 UltraSoC has announced a partnership with Europractice, to bring UltraSoC debug and trace IP for open source RISC-V development to a wider community and to make the company's IP more readily and freely available for academi ...
Electrical Bushings Market Top Companies and Competitive Analysis Report
The global electrical bushings market is expected to display higher growth rate in the upcoming years. Rapid surge in the market is credited to the rapid advancement in power generation industry and growing power demands across the globe. Rapid urbanizati ...
UltraSoC announces next-generation hardware-based cybersecurity products
CAMBRIDGE, UK and SAN JOSE, CA – 8 October 2019 - UltraSoC today announced next-generation hardware-based cybersecurity products that can be used to detect, block and record cyber-attacks in a broad range of applications – from vehicles and factory robots ...


Tommy Hilfiger brings out this classical metal frame with a plastic rubberised arm for extreme comfort and durability. With the Hilfiger logo stamped on the multi-toned arm as a homage to the brand, this is the ideal masculine frame for everyday use.
Very satisfied! Great product backed up by excellent comms from the company.
I'm very happy with these glasses, they fitted me perfectly, definitely worth the money!!


text:
Nicktoons (the channel) News and Discussion Thread 2
Thread starter Neo Yi
Mikurotoro92
Perhaps they are trying to revert back to the original Nicktoons network & this is their way of testing the waters to see if that would work?
The Loud House is too new to have a marathon plus unlike FOP, SBSP, & SJC it doesn't have enough episodes to support one!
I get it...
It's Memorial Day so Viacom wants people to remember when Nicktoons network didn't have any live-action shows on it
superkeegan9100
YTPer, Cartoon Analyst, Artist
Who wants to bet the rest of the summer is live-action marathons?
jaylop97
At least they do a bit of a better job on variety than Disney XD.
D-nice is the man. said:
From zap2it. Memorial Day Weekend is a Marathon of nothing but cartoons. On Saturday, May 27th there's a marathon of the Fairly OddParents from 6 am-10 pm. SpongeBob SquarePants the same day from 10 pm-6 am. On Sunday, May 28th a Sanjay and Craig marathon from 6 am-8 pm, and then there's a marathon of The Loud House from 9 pm-6am. Schedule returns back to normal Memorial Day, May 29th.
unfortunately change has been made: Sat 10PM-6AM has Loud House marathon instead of SpongeBob.
Sun 9PM-6AM SpongeBob marathon, Mon May 29 6AM-6PM Fairly OddParents marathon, rest unchanged marathon still stands
Aspiring animator/filmmaker and Ugliest Man Alive
Bedford, OH
According to Nick and More, 2 new episodes of Rabbids premiere tomorrow 3p-4p
Reactions: 1 person
RDG said:
since we're informed at last minute today for new Rabbids tomorrow and next week Friday, has Nicktoons made promotion for new Rabbids return? It's unknown if new Rabbids will continue Fri Jun 9 since after Mon June 5 schedule is blank (same applies for TeenNick and NickJr), and also we don't know how many episodes in season 3..
That is surely really fast to see.
Premieres of Rabbids are also scheduled next Friday 3p-4p. Also starting today, Yu-Gi-Oh! Arc-V has been removed from Fridays and Saturdays. If you ask me, Nicktoons is giving up the broadcast rights to that show.
According to tvzap2it, I'm not sure if it's an error, but the guide shows on Sun Jun 4 8:25PM-8:30PM TMNT - Teenage Mecha Ninja Turtles, and 8:55PM-9:00PM has TMNT - Don vs Raph. Both are marked as NEW, but I think it's typo
http://tvschedule.zap2it.com/tvlistings/ZCSGrid.do?stnNum=30420&channel=83&aid=tvschedule
kanc said:
5 minutes for an episode definitely would give me a typo vibe.
4Kids4EverFandom
Greenwood,IN USA
Just heard from a Nick Rep about the ARC-V Situation...
Remaining episodes will return in the fall.
Season 2 is still being negotiated.
So now ARC-V's Remaining Episodes of Season 1 will now return in the fall on nicktoons... smh... Unacceptable.
As For season 2 if being negotiated means it will air on Nicktoons or it's rather being negotiated by a different network (LIke Disney XD)?
4Kids4EverFandom said:
I wouldn't be surprised if it did move to another network. Its treatment on Nicktoons has been terrible.
@kanc @jaylop97
Don vs. Raph is a 6-minute TMNT short that Nick debuted at SDCC 2016 - http://nickalive.blogspot.com/2016/07/teenage-mutant-ninja-turtles-don-vs.html
Ghostbuster said:
OK and what about Teenage Mecha Ninja Turtles? Is it a short too?
I can't find any info about it, but it'll probably be a short. Nick will also be debuting a new TMNT short titled "Strike Hard" at Annecy in June.
@kanc Here's Nick's promo for the shorts.
D-nice is the man.
The Only Schedule Changes for Next Week is on Wednesday, June 7th there's the Fairly OddParents reruns from 1-4 pm, I believe it replaces Sanjay and Craig in that slot. Yu-Gi-Oh! Arc-V reruns return Friday at 4 pm and Saturday at 12 pm. The Fifth Season of TMNT reruns encores begin Sunday, June 11th at 8 pm.
Wed Jun 7 8PM Nicktoons airs Nickelodeon's Sizzling Summer Camp Special, 9pm-10:30PM FOP - Channel Chasers
Starting Fridays 9pm-11pm movies, and encore movie at 12am
Sundays 8PM-9pm will air TMNT shorts as well as TMNT reruns as mentioned
Have you noticed that Nick sometimes replaces Nicktoons' bumpers with their own? That's so lazy and confusing!
^Though it definitely saves them from wasting money on a channel that doesn't pick up to many viewers.
I've always thought Nicktoons had better bumpers than Nick itself
Nick Jr News And Discussion Thread Part 2
Toons Fan
Does anyone feel like nicktoons channel is lacking variety these days?
Santana434
JMTV Studios
"Marvel's What If...? (Disney+)" News & Discussion Thread, Part 2 (Spoilers)
RoyalRubble
The Marvel Animation Forum
Perla Magica
Francisque
GermanGem


text:
Even with 18,000 new votes on its roll courtesy of RNC,
Keadaan Terkawal di Lembah Pantai
So says our 'defection proof' MP
with thanks to Malaysian Insider
In Lembah Pantai, Nurul Izzah fights Nong Chik with 'flowers'
BY EMILY DING AND BOO SU-LYN
KUALA LUMPUR, April 17 — A residents' initiative planting coloured flags to support Nurul Izzah Anwar is now part of the incumbent Lembah Pantai MP's campaign to defend her seat against Umno strongman Datuk Raja Nong Chik Zainal.
Miniature blue, white, and red flags — the PKR vice-president's party colours — as well as electoral reform group Bersih's bright yellow were planted at a Bangsar roundabout as part of residents' "Malaysian Spring" campaign for political change over the weekend.
But the same group had to stand up to Kuala Lumpur City Hall (DBKL) officers who had plucked the "flowers" hours later on Sunday, citing transgression of city by-laws.
The residents later replanted the flags, after criticising the City Hall officers for ignoring similar transgressions by Nong Chik's Barisan Nasional (BN) campaign officials with their billboards and flags.
Nurul Izzah said the idea to make the "Malaysian Spring" as part of her campaign came after "one of the residents called me and said, 'We're going to fight for this place'."
"Then the suggestion came from resident lawyers — they said one of the ways to mitigate this is to adopt it formally as part of my campaign material. So if you (DBKL) would like to remove them (the flags), you first and foremost have to remove the other paraphernalia belonging to Barisan Nasional," the 32-year-old lawmaker told The Malaysian Insider.
Nurul Izzah — the eldest daughter of Opposition Leader Datuk Seri Anwar Ibrahim — is facing interim federal territories and urban wellbeing minister Nong Chik in the May 5 polls, the second minister she is contesting against after defeating then-women, family and community development minister Datuk Seri Shahrizat Abdul Jalil in Election 2008.
Several residents described the incumbent Lembah Pantai lawmaker as "approachable".Several Bangsar residents whom The Malaysian Insider spoke to called Nurul Izzah an "approachable" politician, while some — who were not even living in Bangsar — said they chose to volunteer for the "Malaysian Spring" campaign to end corruption and government abuse of power.
To date, 18,400 "flowers" have been planted, with Malaysian Spring organisers led by landscape architect Ng Seksan planning to plant a total of 28 million "flowers" in the country.
"It shows that she actually cares what we think as residents. She's adopting a residents' initiative, not the other way round," said pharmacist Mithra Singam.
Mithra also pointed out that DBKL's actions might have tipped some voters towards Pakatan Rakyat (PR).
"Some people may be neutral at first, but they see what happened and they get agitated and irritated and that's when they start reacting and favour the opposition more," she said.
Her sister, Simitha, an activist, said Nurul Izzah was a very helpful MP.
"Everything is just one tweet away with her. You know, there's a pothole, you tweet her, you send her the picture and she'll get it fixed," said Simitha, referring to Nurul Izzah's Twitter account, @n_izzah.
Retired landscape architect Carolyn Lau said she was happy that Nurul Izzah had made "Malaysian Spring" part of her campaign.
"She hasn't had the financial goldmine, the piggy bank that the other party has, and she's had to do things as effective and efficiently as she can," said the 48-year-old.
Lembah Pantai, which has 72,553 voters, is a constituency of sharp contrasts, comprising the wealthy Bangsar where urbanites eat expensive macaroons, along with the low-cost homes in Kampung Pantai Dalam and Bukit Kerinchi.
Since his appointment as FT minister in 2009, Nong Chik has worked on various projects to uplift the constituency that includes the upscale Pantai and Bangsar neighbourhoods, as well as poorer sections in Brickfields and Pantai Dalam.
But businesswoman Shariffah Jamalah, who has been running a despatch service in Bangsar for 25 years, dismissed 60-year-old Nong Chik as "arrogant", compared to the "humble" Nurul Izzah.
"Raja Nong Chik only appears when election is coming," she told The Malaysian Insider yesterday.
"I was a BN supporter for three to four terms. I became a polling agent. But when I talk to them about my problems, they don't listen. You don't listen and you want us to support you," Shariffah, 56, said.
Shariffah pointed out that Rela members were only deployed in Bangsar recently to fight a spate of crime in Lucky Garden in Bangsar, like snatch theft and robberies, saying: "Don't do it just during election time."
Pak Zawi Friday, 19 April 2013 at 11:11:00 GMT+10
Way to go the people of Lembah Pantai and Izzah. Down with the plunderer.
Way to go Nurul! Looking forward to the spread of the Malaysian Spring fever to other constituencies soon!
Malchindian Friday, 19 April 2013 at 13:52:00 GMT+10
We concur wholeheartedly to a peaceful evolution of Malaysia's political landscape! Go Nurul Go!
Pak Idrus Friday, 19 April 2013 at 14:24:00 GMT+10
Steadylah brother. Tak balik mengundi!


text:
A statue of John Batchelor stands in The Hayes, Cardiff, Wales in recognition of the businessman and Liberal politician, John Batchelor (1820 – 1883). The statue was erected in 1886.

The statue became Grade II listed in 1975.

Background
John Batchelor was born in Newport, the son of a Congregationalist timber merchant and shipbuilder. He was apprenticed in ship building and set up in business with his brother in Cardiff. With his Liberal politics, Batchelor was a successful opponent of the dominant Marquesses of Bute in politics and business. He was an eloquent orator. Batchelor became a councillor and mayor of Cardiff when the Liberals took control of Cardiff Town Council in 1853. He helped bring a clean water supply and sewerage system to the town.

Batchelor's ship building business eventually failed after being impeded and undermined by the Bute Estate, who dominated Cardiff Docks. Local non-conformists raised £3,700 to support Batchelor. Batchelor promoted development of Penarth Docks, including proposals for a barrage across the River Taff (which the Butes also opposed). Batchelor retired as a broken man and died on 29 May 1883.

Statue
Shortly after his death, £1,000 had been raised to raise a statue to John Batchelor. An application to site the memorial statue on The Hayes was approved by the Town Council in January 1885. The statue was designed by James Milo Griffith and was unveiled in The Hayes, Cardiff, on 16 October 1886, in a ceremy performed by prominent businessmen and Members of Parliament. The ceremony was arranged for a Saturday to allow Cardiff's working population to attend. The statue was poignantly located in front of Cardiff's Free Library, which Batchelor had campaigned for.

The statue is cast in bronze, Batchelor standing with one leg in front of the other and his right arm raised. The statue stands on a tall stone pedestal with the words "The Friend of Freedom" written on the front. The statue is  high and the pedestal is  in height. Batchelor's relatives were said to approve of the likeness. The statue was originally much closer to the library than it is today.

Within a few months of its unveiling the local Conservatives had launched a campaign to remove the statue. A petition was rejected by the Town Council but, all the same, Conservative William Thorn defaced the statue with yellow paint and tar. It was defaced for a third time, with red and blue paint, in January 1887. Conservative solicitor, T. H. Ensor, wrote a scathing piece in the Western Mail, suggesting the words "Friend of Freedom" be replaced with "traitor to the Crown... hater of the clergy... sincerely mourned by unpaid creditors". He was sued unsuccessfully for libel.

In 2010 Conservative member of the Welsh Assembly, David Melding, argued that the statue should be moved elsewhere, replaced by someone of national rather than local importance in the centre of Cardiff, such as David Lloyd George. Russell Goodway of Cardiff Council responded by saying there were more important priorities than relocating statues.

References

External links

1886 establishments in Wales
Batchelor, John
Batchelor, John


text:
Antibody catalysis of some organic and biochemical reactions: Dissertation
Jari Yli-Kauhaluoma
Research output: Thesis › Dissertation › Collection of Articles
Antibodies are immunoglobulins that bind to stable ground-state molecules and recognise their respective antigens with high affinity and high specificity. Enzymes in turn are natural catalysts that bind and stabilise selectively the transition-state of the reaction and accelerate the rate of a (bio)chemical reaction by lowering the free energy of activation. Enzymes are also able to act as "entropy traps" in reducing the rotational and translational degrees of freedom that are prerequisites for the formation of the activated complex between the reactants. In addition, enzymes use general acid and base catalysis, nucleophiles and co-factors in enhancing the rates of reactions. It was nearly fifty years ago when Linus Pauling first proposed in his lecture entitled "Chemical Achievement and Hope for the Future" that antibodies binding the ground state molecules might act as enzyme-type catalysts for chemical reactions. Twenty-one years later, William Jencks suggested that it should be possible to obtain an antibody with enzymatic properties by raising it against the antigen that resembles the transition-state of the reaction. It is only eleven years ago that the first reports of the catalytic antibodies emerged from the laboratories of Richard Lerner and Peter Schultz. Indeed, the antibodies elicited against stable, natural or synthetic transition-state analogues of numerous reactions have been found to possess enzyme-like activities. These catalytic antibodies generally display the Michaelian type saturation kinetics, competitive inhibition by the transition-state analogue, selective binding to the transition-state and remarkable substrate specificities. In the present investigation, structurally different antigens (haptens) were used to study whether it was possible to obtain antibody catalysts for the acyl-transfer, Diels Alder and peptidyl-prolyl cis-trans isomerisation reactions. Acyl-transfer reactions, such as hydrolytic reactions are important transformations both in bio-chemistry and synthetic organic chemistry; the Diels Alder reaction is synthetically useful in constructing substituted cyclohexenes; and the peptidyl-prolyl cis-trans isomerisation reaction is a highly substantial biochemical reaction which plays a significant role in protein folding, transport and transmembrane signalling. Monoclonal antibodies were raised against two a-keto amide moiety containing antigens. They were anticipated to induce antibodies for hydrolytic acyl-transfer reactions, i.e. ester and amide hydrolyses. a-Keto amide substructures found in natural macrolides such as FK506, rapamycin and cyclotheonamide A are known to mimic the twisted amide bond that is one possible transition-state for the amide bond hydrolysis. During the study, a new, synthetically useful concurrent alkylative de-carbonylation and decarboxylation reaction of methoxy-substituted 3-phenyl-2-oxo-propanoic acids was discovered. It turned out to be a viable method for the preparation of isopropyl anisoles and veratroles, producing them in high yields. The elicitation of antibodies against the freely-rotating, lipophilic and highly aromatic ferrocene haptens as loose transition-state mimics was successful. Both endo and exo selective antibodies catalysing the Diels Alder reaction between 4-carboxy-benzyl trans-1,3-butadiene-1-carbamate and N,N-dimethylacrylamide were found. High regio-, diastereo- and enantioselectivities and no product inhibition were observed. Moreover, the found Diels Alderases had effective molarities comparable to those of antibodies elicited against the constrained bicyclo[2.2.2]octene haptens. The dicarbonyl moiety in natural products FK506 and rapamycin and less complex pyruvylamides adopts an orthogonal conformation and possibly serves as a twisted-amide mimic. The a-keto Val-Pro-Phe hapten was anticipated to induce anti-body binding sites that were complementary to the twisted a-keto amide functionality and of hydrophobic character. Indeed, two antibodies were found to catalyse the cis to trans isomerisation of the fluorophoric tripeptides and the 4-nitroanilide substrates as characterised using both direct fluorescence quench and chymotrypsin-coupled assays, respectively. Both catalyst showed competitive inhibition by the antigen derivative, and the product inhibition, i.e. binding to the trans isomer, did not appear to be significant. In catalysis and binding the peptide substrates, factors other than simple hydrophobic interactions are possibly involved, such as transition-state stabilisation and ground-state destabilisation.
Doctor Degree
University of Helsinki
Janda, Kim D., Supervisor, External person
Haptens
Isomerization
Catalytic Antibodies
Ground state
Anisoles
Cyclohexenes
Carbonylation
Nucleophiles
Dies-Alder reactions
cis-trans-isomerases
Yli-Kauhaluoma, J. (1997). Antibody catalysis of some organic and biochemical reactions: Dissertation. Espoo: VTT Technical Research Centre of Finland.
Yli-Kauhaluoma, Jari. / Antibody catalysis of some organic and biochemical reactions : Dissertation. Espoo : VTT Technical Research Centre of Finland, 1997. 125 p.
@phdthesis{42169333246348d18bb66a603427e3c6,
title = "Antibody catalysis of some organic and biochemical reactions: Dissertation",
abstract = "Antibodies are immunoglobulins that bind to stable ground-state molecules and recognise their respective antigens with high affinity and high specificity. Enzymes in turn are natural catalysts that bind and stabilise selectively the transition-state of the reaction and accelerate the rate of a (bio)chemical reaction by lowering the free energy of activation. Enzymes are also able to act as {"}entropy traps{"} in reducing the rotational and translational degrees of freedom that are prerequisites for the formation of the activated complex between the reactants. In addition, enzymes use general acid and base catalysis, nucleophiles and co-factors in enhancing the rates of reactions. It was nearly fifty years ago when Linus Pauling first proposed in his lecture entitled {"}Chemical Achievement and Hope for the Future{"} that antibodies binding the ground state molecules might act as enzyme-type catalysts for chemical reactions. Twenty-one years later, William Jencks suggested that it should be possible to obtain an antibody with enzymatic properties by raising it against the antigen that resembles the transition-state of the reaction. It is only eleven years ago that the first reports of the catalytic antibodies emerged from the laboratories of Richard Lerner and Peter Schultz. Indeed, the antibodies elicited against stable, natural or synthetic transition-state analogues of numerous reactions have been found to possess enzyme-like activities. These catalytic antibodies generally display the Michaelian type saturation kinetics, competitive inhibition by the transition-state analogue, selective binding to the transition-state and remarkable substrate specificities. In the present investigation, structurally different antigens (haptens) were used to study whether it was possible to obtain antibody catalysts for the acyl-transfer, Diels Alder and peptidyl-prolyl cis-trans isomerisation reactions. Acyl-transfer reactions, such as hydrolytic reactions are important transformations both in bio-chemistry and synthetic organic chemistry; the Diels Alder reaction is synthetically useful in constructing substituted cyclohexenes; and the peptidyl-prolyl cis-trans isomerisation reaction is a highly substantial biochemical reaction which plays a significant role in protein folding, transport and transmembrane signalling. Monoclonal antibodies were raised against two a-keto amide moiety containing antigens. They were anticipated to induce antibodies for hydrolytic acyl-transfer reactions, i.e. ester and amide hydrolyses. a-Keto amide substructures found in natural macrolides such as FK506, rapamycin and cyclotheonamide A are known to mimic the twisted amide bond that is one possible transition-state for the amide bond hydrolysis. During the study, a new, synthetically useful concurrent alkylative de-carbonylation and decarboxylation reaction of methoxy-substituted 3-phenyl-2-oxo-propanoic acids was discovered. It turned out to be a viable method for the preparation of isopropyl anisoles and veratroles, producing them in high yields. The elicitation of antibodies against the freely-rotating, lipophilic and highly aromatic ferrocene haptens as loose transition-state mimics was successful. Both endo and exo selective antibodies catalysing the Diels Alder reaction between 4-carboxy-benzyl trans-1,3-butadiene-1-carbamate and N,N-dimethylacrylamide were found. High regio-, diastereo- and enantioselectivities and no product inhibition were observed. Moreover, the found Diels Alderases had effective molarities comparable to those of antibodies elicited against the constrained bicyclo[2.2.2]octene haptens. The dicarbonyl moiety in natural products FK506 and rapamycin and less complex pyruvylamides adopts an orthogonal conformation and possibly serves as a twisted-amide mimic. The a-keto Val-Pro-Phe hapten was anticipated to induce anti-body binding sites that were complementary to the twisted a-keto amide functionality and of hydrophobic character. Indeed, two antibodies were found to catalyse the cis to trans isomerisation of the fluorophoric tripeptides and the 4-nitroanilide substrates as characterised using both direct fluorescence quench and chymotrypsin-coupled assays, respectively. Both catalyst showed competitive inhibition by the antigen derivative, and the product inhibition, i.e. binding to the trans isomer, did not appear to be significant. In catalysis and binding the peptide substrates, factors other than simple hydrophobic interactions are possibly involved, such as transition-state stabilisation and ground-state destabilisation.",
keywords = "antibodies, catalysts, biochemical analysis, haptens, Dies-Alder reactions, acyltransferases, cis-trans-isomerases",
author = "Jari Yli-Kauhaluoma",
isbn = "951-38-4957-0",
series = "VTT Publications",
school = "University of Helsinki",
Yli-Kauhaluoma, J 1997, 'Antibody catalysis of some organic and biochemical reactions: Dissertation', Doctor Degree, University of Helsinki, Espoo.
Antibody catalysis of some organic and biochemical reactions : Dissertation. / Yli-Kauhaluoma, Jari.
Espoo : VTT Technical Research Centre of Finland, 1997. 125 p.
T1 - Antibody catalysis of some organic and biochemical reactions
T2 - Dissertation
AU - Yli-Kauhaluoma, Jari
N2 - Antibodies are immunoglobulins that bind to stable ground-state molecules and recognise their respective antigens with high affinity and high specificity. Enzymes in turn are natural catalysts that bind and stabilise selectively the transition-state of the reaction and accelerate the rate of a (bio)chemical reaction by lowering the free energy of activation. Enzymes are also able to act as "entropy traps" in reducing the rotational and translational degrees of freedom that are prerequisites for the formation of the activated complex between the reactants. In addition, enzymes use general acid and base catalysis, nucleophiles and co-factors in enhancing the rates of reactions. It was nearly fifty years ago when Linus Pauling first proposed in his lecture entitled "Chemical Achievement and Hope for the Future" that antibodies binding the ground state molecules might act as enzyme-type catalysts for chemical reactions. Twenty-one years later, William Jencks suggested that it should be possible to obtain an antibody with enzymatic properties by raising it against the antigen that resembles the transition-state of the reaction. It is only eleven years ago that the first reports of the catalytic antibodies emerged from the laboratories of Richard Lerner and Peter Schultz. Indeed, the antibodies elicited against stable, natural or synthetic transition-state analogues of numerous reactions have been found to possess enzyme-like activities. These catalytic antibodies generally display the Michaelian type saturation kinetics, competitive inhibition by the transition-state analogue, selective binding to the transition-state and remarkable substrate specificities. In the present investigation, structurally different antigens (haptens) were used to study whether it was possible to obtain antibody catalysts for the acyl-transfer, Diels Alder and peptidyl-prolyl cis-trans isomerisation reactions. Acyl-transfer reactions, such as hydrolytic reactions are important transformations both in bio-chemistry and synthetic organic chemistry; the Diels Alder reaction is synthetically useful in constructing substituted cyclohexenes; and the peptidyl-prolyl cis-trans isomerisation reaction is a highly substantial biochemical reaction which plays a significant role in protein folding, transport and transmembrane signalling. Monoclonal antibodies were raised against two a-keto amide moiety containing antigens. They were anticipated to induce antibodies for hydrolytic acyl-transfer reactions, i.e. ester and amide hydrolyses. a-Keto amide substructures found in natural macrolides such as FK506, rapamycin and cyclotheonamide A are known to mimic the twisted amide bond that is one possible transition-state for the amide bond hydrolysis. During the study, a new, synthetically useful concurrent alkylative de-carbonylation and decarboxylation reaction of methoxy-substituted 3-phenyl-2-oxo-propanoic acids was discovered. It turned out to be a viable method for the preparation of isopropyl anisoles and veratroles, producing them in high yields. The elicitation of antibodies against the freely-rotating, lipophilic and highly aromatic ferrocene haptens as loose transition-state mimics was successful. Both endo and exo selective antibodies catalysing the Diels Alder reaction between 4-carboxy-benzyl trans-1,3-butadiene-1-carbamate and N,N-dimethylacrylamide were found. High regio-, diastereo- and enantioselectivities and no product inhibition were observed. Moreover, the found Diels Alderases had effective molarities comparable to those of antibodies elicited against the constrained bicyclo[2.2.2]octene haptens. The dicarbonyl moiety in natural products FK506 and rapamycin and less complex pyruvylamides adopts an orthogonal conformation and possibly serves as a twisted-amide mimic. The a-keto Val-Pro-Phe hapten was anticipated to induce anti-body binding sites that were complementary to the twisted a-keto amide functionality and of hydrophobic character. Indeed, two antibodies were found to catalyse the cis to trans isomerisation of the fluorophoric tripeptides and the 4-nitroanilide substrates as characterised using both direct fluorescence quench and chymotrypsin-coupled assays, respectively. Both catalyst showed competitive inhibition by the antigen derivative, and the product inhibition, i.e. binding to the trans isomer, did not appear to be significant. In catalysis and binding the peptide substrates, factors other than simple hydrophobic interactions are possibly involved, such as transition-state stabilisation and ground-state destabilisation.
AB - Antibodies are immunoglobulins that bind to stable ground-state molecules and recognise their respective antigens with high affinity and high specificity. Enzymes in turn are natural catalysts that bind and stabilise selectively the transition-state of the reaction and accelerate the rate of a (bio)chemical reaction by lowering the free energy of activation. Enzymes are also able to act as "entropy traps" in reducing the rotational and translational degrees of freedom that are prerequisites for the formation of the activated complex between the reactants. In addition, enzymes use general acid and base catalysis, nucleophiles and co-factors in enhancing the rates of reactions. It was nearly fifty years ago when Linus Pauling first proposed in his lecture entitled "Chemical Achievement and Hope for the Future" that antibodies binding the ground state molecules might act as enzyme-type catalysts for chemical reactions. Twenty-one years later, William Jencks suggested that it should be possible to obtain an antibody with enzymatic properties by raising it against the antigen that resembles the transition-state of the reaction. It is only eleven years ago that the first reports of the catalytic antibodies emerged from the laboratories of Richard Lerner and Peter Schultz. Indeed, the antibodies elicited against stable, natural or synthetic transition-state analogues of numerous reactions have been found to possess enzyme-like activities. These catalytic antibodies generally display the Michaelian type saturation kinetics, competitive inhibition by the transition-state analogue, selective binding to the transition-state and remarkable substrate specificities. In the present investigation, structurally different antigens (haptens) were used to study whether it was possible to obtain antibody catalysts for the acyl-transfer, Diels Alder and peptidyl-prolyl cis-trans isomerisation reactions. Acyl-transfer reactions, such as hydrolytic reactions are important transformations both in bio-chemistry and synthetic organic chemistry; the Diels Alder reaction is synthetically useful in constructing substituted cyclohexenes; and the peptidyl-prolyl cis-trans isomerisation reaction is a highly substantial biochemical reaction which plays a significant role in protein folding, transport and transmembrane signalling. Monoclonal antibodies were raised against two a-keto amide moiety containing antigens. They were anticipated to induce antibodies for hydrolytic acyl-transfer reactions, i.e. ester and amide hydrolyses. a-Keto amide substructures found in natural macrolides such as FK506, rapamycin and cyclotheonamide A are known to mimic the twisted amide bond that is one possible transition-state for the amide bond hydrolysis. During the study, a new, synthetically useful concurrent alkylative de-carbonylation and decarboxylation reaction of methoxy-substituted 3-phenyl-2-oxo-propanoic acids was discovered. It turned out to be a viable method for the preparation of isopropyl anisoles and veratroles, producing them in high yields. The elicitation of antibodies against the freely-rotating, lipophilic and highly aromatic ferrocene haptens as loose transition-state mimics was successful. Both endo and exo selective antibodies catalysing the Diels Alder reaction between 4-carboxy-benzyl trans-1,3-butadiene-1-carbamate and N,N-dimethylacrylamide were found. High regio-, diastereo- and enantioselectivities and no product inhibition were observed. Moreover, the found Diels Alderases had effective molarities comparable to those of antibodies elicited against the constrained bicyclo[2.2.2]octene haptens. The dicarbonyl moiety in natural products FK506 and rapamycin and less complex pyruvylamides adopts an orthogonal conformation and possibly serves as a twisted-amide mimic. The a-keto Val-Pro-Phe hapten was anticipated to induce anti-body binding sites that were complementary to the twisted a-keto amide functionality and of hydrophobic character. Indeed, two antibodies were found to catalyse the cis to trans isomerisation of the fluorophoric tripeptides and the 4-nitroanilide substrates as characterised using both direct fluorescence quench and chymotrypsin-coupled assays, respectively. Both catalyst showed competitive inhibition by the antigen derivative, and the product inhibition, i.e. binding to the trans isomer, did not appear to be significant. In catalysis and binding the peptide substrates, factors other than simple hydrophobic interactions are possibly involved, such as transition-state stabilisation and ground-state destabilisation.
KW - antibodies
KW - catalysts
KW - biochemical analysis
KW - haptens
KW - Dies-Alder reactions
KW - acyltransferases
KW - cis-trans-isomerases
M3 - Dissertation
SN - 951-38-4957-0
T3 - VTT Publications
CY - Espoo
Yli-Kauhaluoma J. Antibody catalysis of some organic and biochemical reactions: Dissertation. Espoo: VTT Technical Research Centre of Finland, 1997. 125 p.
http://www.vtt.fi/inf/pdf/publications/1997/p299.pdf