Brian Ruffell joined Dr. Johnson's lab as a graduate student in 2002, on a project to investigate the function of chondroitin sulfate addition to CD44. Brian's work examined the function of this post-translational modification in macrophages and T cells where he discovered a major role for chondroitin sulfate in regulating hyaluronan binding by CD44. Dr. Ruffell is currently at the University of California, San Francisco, pursuing his research interests in the role of inflammation in cancer progression and metastasis.
Dr. Johnson became interested in the function of cell surface molecules during her PhD. in Biochemistry at the University of Dundee, under the tutelage of Dr. Peter Garland and Dr. Roger Clegg. In her post-doc, she entered the field of Immunology in Dr. Alan William's lab at the University of Oxford in 1983, where she characterized and cloned the lymphocyte cell surface antigen, CD8. In 1987 she went to the Salk Institute to investigate the function of the leukocyte specific antigen, CD45, in Dr. Ian Trowbridge's lab. It was here where she was inspired by the work of Dr. Jayne Lesley and Dr. Bob Hyman who worked in the adjacent lab, and soon became fascinated by the cell adhesion molecule, CD44. She began her own lab at the University of British Columbia (UBC) in Vancouver in 1991, initially working on the molecular function of CD44 and CD45 in T lymphocytes. Dr. Johnson is now professor and co-director of the Infection, Inflammation and Immunity (I3) research group at UBC and present research interests include: the regulation of innate and adaptive immune responses by CD45; how CD44 interactions with hyaluronan regulate the immune response; the regulation of leukocyte adhesion and migration by CD44; and how CD44 contributes to the inflammation process.
The extracellular matrix glycosaminoglycan, hyaluronan (HA), is an unbranched polymer composed of repeating glucuronic acid and N-acetyl glucosamine disaccharide units. Unlike other glycosaminoglycans that are made as proteoglycans, HA is not linked to a protein core and is instead synthesized by up to three hyaluronan synthases that reside on the inner surface of the plasma membrane. Transportation of HA to the cell surface during synthesis allows up to 25,000 disaccharides to be linked together, creating a polysaccharide with a molecular mass that normally ranges between 106-107 Daltons. HA is a critical component of the extracellular matrix (ECM), demonstrated by the embryonic lethality of hyaluronan synthase-2 knockout mice 1. HA is bound by a number of ECM and cell surface proteins. These HA-binding proteins are termed hyaladherins and share a homologous HA binding domain with the Link protein, which is referred to as the Link homology domain 2. Examples include aggrecan, versican, and tumor necrosis factor alpha stimulated gene 6 (TSG-6, 3). Although there is an endocytic receptor for HA (HARE) present in the liver, spleen, and lymph nodes 4, it does not possess a Link homology domain. The Link homology domain is also absent from SHAP (inter alpha trypsin inhibitor heavy chain), a serum protein that can bind covalently to HA 5. Two transmembrane hyaladherins have been described, and one of these, lymphatic vessel endothelial receptor-1 (LYVE-1) is predominantly expressed by lymphatic endothelium 6. The other, more widely expressed molecule, CD44, has been implicated in a range of processes that are largely, but not exclusively, dependent on its function as an HA-binding protein 7.
CD44, the primary cell surface receptor for HA, is present at varying levels on the majority of cells in the immune system 8. These cells predominantly express the standard (CD44s) or hematopoietic (CD44H) form of CD44. Additional isoforms of CD44 can be expressed by alternative splicing of 10 variably expressed exons, but these are generally a minor component of the total CD44 expressed in leukocytes and will not be further discussed here. Even in the absence of these additional isoforms, there is a high degree of heterogeneity in the structure of CD44 due to extensive post-translational modifications. CD44 can be modified by N- and O-linked glycosylation 9 and glycosaminoglycan addition 9-11, and their addition and composition is dynamically regulated by the cell 12-15. The carbohydrate moieties of CD44 can function independently as blood group antigens on red blood cells 16 and as ligands for E-selectin on neutrophils 17 and E- and L-selectin on human hematopoietic progenitor cells 18,19.
CD44 has an amino terminal Link module homology domain that is responsible for binding HA. Crystallization of the amino terminal region of CD44 with HA shows HA binding to a shallow groove in the Link homology domain in an interaction that is dominated by hydrogen bonds and van der Waals forces 20. Notably, CD44 was crystallized in two conformational states: one with HA bound and one without. In addition, the conformation of the HA oligosaccharide was slightly different when it bound to CD44. In the HA binding conformation of CD44, a number of residues showed a slight shift, including a critical arginine residue that moved to form a closer interaction with HA 20. This raises the interesting possibility that CD44 may exist in two conformational states in cells (a high and low affinity state), which may help explain the different HA binding states of CD44 observed in lymphoid cells 21. However at this point, it is not known whether different conformational states of CD44 exist in cells and if so, whether HA itself or other factors such as the post-translational modifications of CD44 induce or help stabilize a particular conformation of CD44.
The CD44-HA interaction can lead to a transient, rolling interaction under flow conditions or a more stable cell-cell or cell-matrix interaction under static conditions. The extent of HA binding by leukocytes is determined by the complex interplay of multivalent interactions between CD44 and HA that is dependent upon the amount and density of CD44 at the cell surface, as well as its activation state, which is determined by cell specific factors 22. These are discussed in more detail in the next section.
CD44 can exist in three different states in lymphoid cells with respect to HA binding: non-binding, inducible (by the CD44 IRAW14 mAb) and constitutive 23. These states may arise due to differential glycosylation or glycosaminoglycan addition to CD44 12. Expression levels of CD44 can further enhance HA binding once a distinct threshold level has been obtained. In addition to post-translational modifications of CD44, the presentation of HA can affect how these two molecules interact.
N and O-linked carbohydrates, as well as glycosaminoglycan addition to CD44 can influence its ability to bind HA 12 and reviewed in 24-26. These modifications can have both positive and negative effects, depending on the type of glycosylation involved and the cell type examined (see Table 1). While all of the five N-glycosylation sites on CD44 reside in the amino terminal Link homology domain, the majority of O-linked glycosylation is thought to occur in the membrane proximal stem-like region of CD44 26, where the site of chondroitin sulfate addition occurs 27,28. Generally, HA binding increases with reduced N-glycosylation, although this is cell type dependent. For example, mutation of N1 and N5 glycosylation sites led to constitutive binding in inducible cell lines, yet carbohydrate addition at these sites did not prevent constitutive HA binding in other cells 12,13,29. This implies that other regulatory mechanisms can overcome the negative regulatory effect of N-glycosylation. At least part of the negative effect of N-linked glycans on CD44 may be due to the presence of terminal sialic acid residues, as their removal enhances HA binding 13,30. Some N-glycosylation sites are close to the HA binding groove, raising the possibility that glycosylation at these sites could directly prevent HA binding by steric hindrance or charge repulsion, or could interact with residues on CD44 close to the HA binding groove and reduce the affinity of CD44 for HA. Alternatively, carbohydrates located away from the binding groove may affect HA binding indirectly, by interacting with CD44 residues and stabilizing an unfavorable HA binding conformation of CD44. Chondroitin sulfate addition to CD44 can also negatively affect HA binding 28. Addition of chondroitin sulfate occurs at serine 180 in human CD44 28, which is located outside the HA binding domain of CD44. It may negatively regulate HA binding either indirectly by affecting CD44 conformation, or directly by competing for HA binding, as chondroitin sulfate modified proteins can bind to the HA binding site of CD44 31,32.
Providing that CD44 is capable of binding HA and that a threshold level of CD44 has been obtained, HA binding increases with increasing CD44 expression 29. HA binding can be augmented by the association of the cytoplasmic domain of CD44 with the actin cytoskeleton 21,33, as disruption of actin polymerization in monocytes 34 and stimulated T cells 35 reduces HA binding. The association of CD44 with the cytoskeleton can occur via an interaction with phosphorylated ERM proteins 36 and this may influence HA binding, at least in some cell types 34. CD44 and phosphorylated ERM proteins co-localize in TNFα-activated monocytes and prevention of ERM phosphorylation by a kinase inhibitor reduces HA binding. An interaction of CD44 with the cytoskeleton may facilitate clustering and enhance the avidity of CD44 for HA, or it may induce a conformational change in CD44 that increases its affinity for HA. In some cell lines, the CD44 IRAW14 mAb, but not its Fab fragment, can induce HA binding 37, suggesting that dimerization or an antibody induced conformational change facilitates HA binding. Fig. 1 illustrates the different mechanisms that may regulate CD44 binding to HA. They may occur alone or in combination, and depend on the activation state of the cell and the cell type. The challenge now is to identify which mechanisms are evoked under physiological conditions.
Potential mechanisms to regulate HA binding by CD44. (A) Steric hindrance by post-translational modifications such as N-glycosylation (N-CHO) or chondroitin sulfate (CS) addition to CD44 may prevent HA binding or reduce the affinity of CD44 for HA. (B) Local increases in CD44 expression caused by clustering may enhance the avidity of the interaction. (C) Post-translational modifications, such as N-CHO or CS may cause conformational changes that have different binding affinities for HA. (D) Increases in CD44 expression can increase HA binding by increasing the avidity of the interaction, provided that an initial threshold level has been met and that negative regulators of HA binding are not preventing the interaction.
Sialic acid residues negatively regulate HA binding in human peripheral blood monocytes (PBM) 38. This was demonstrated during LPS-induced HA binding, as sialidase activity was increased and inclusion of a sialidase inhibitor reduced HA binding 38. TNFα also induces HA binding in human monocytic cells 14,39 and although this correlates with increased carbohydrate sulfation of CD44 15, it has not been proven that this is directly responsible for the induction of HA binding. Likewise, it has not been proven that changes in N-glycosylation contribute to TNFα induced HA binding. However, there is accumulating evidence supporting a physiological role for chondroitin sulfate addition in regulating HA binding. TNFα reduces chondroitin sulfate addition to CD44 and induces HA binding in mouse bone marrow-derived macrophages (unpublished data) and inhibition of glycosaminoglycan addition in both mouse bone marrow-derived macrophages and human monocytic cells 40, or specific prevention of chondroitin sulfate addition by mutation of CD44 in human Jurkat T cells 41 induces HA binding. Therefore, sialic acid and chondroitin sulfate are two physiological regulators of HA binding in monocytes/macrophages. The physiological regulators in other leukocytes remain to be established.
Inflammatory stimuli upregulate HA expression on endothelial cells 42 providing one mechanism to facilitate a CD44-dependent leukocyte interaction at inflammatory sites. Inflammatory conditions also upregulate TSG-6 3, which can bind to HA and enhance the interaction of HA with CD44, possibly by crosslinking HA and increasing the avidity of the interaction 43. SHAP, a protein present in serum, can covalently bind to HA and enhance its binding to CD44 44. This may have physiological consequences, as U937 monocytic cells and peripheral blood mononuclear cells, which do not normally bind significant levels of soluble fluoresceinated HA (Fl-HA), can bind to SHAP-associated HA cables produced by poly I:C stimulation of smooth muscle cells 45,46. In addition, an increase in the expression of SHAP upon LPS-induced hepatic injury was speculated to account for the CD44:HA dependent sequestration of neutrophils in liver sinusoids, as levels of HA did not change and CD44 on neutrophils was not upregulated to bind HA 47. These studies imply that HA binding proteins can influence how HA is presented to CD44, which in turn, may affect the affinity or avidity of the interaction. Together, these data indicate that the CD44:HA interaction can be regulated by modifications to both CD44 and HA.
CD44 is expressed on early hematopoietic progenitor cells (including the hematopoietic stem cell) and has been implicated in the homing of hematopoietic stem cells after bone marrow transplantation 48,49. Despite this and evidence that HA is a component of the stem cell niche in the bone marrow 50, CD44 null mice appear to develop normally 51,52. CD44 is expressed on early thymocyte progenitors in the thymus and is down regulated during thymocyte development. Initial blocking studies with CD44 mAbs suggested a role for CD44 in thymic progenitor homing 53, but this was not apparent in the CD44 null mice 51, leaving the functional significance of CD44 on these early T cell progenitors unknown. CD44 is expressed at low levels on naïve T cells exiting the thymus in C57Bl/6 mice, although expression levels vary with the strain of mouse (reviewed in 8). Again, initial studies in the human suggesting a role for CD44 in lymphocyte homing 54 were not substantiated in the CD44 null mouse 51 and competition experiments between wild type and CD44-deficient lymphocytes show similar trafficking patterns in naïve recipients 55. Thus, CD44 appears to be dispensable for normal immune cell development and homeostasis in the mouse, although it is possible that the lack of CD44 may be compensated for in the CD44 null mouse by other adhesion molecules or by RHAMM, as has been suggested 56. Although CD44 is expressed on most cells of the immune system to varying degrees, the majority of cells do not bind detectable levels of Fl-HA under normal homeostatic conditions. CD44 levels are increased in monocytes upon exposure to inflammatory agents 39 and in T cells upon antigen-induced activation in C57Bl/6 mice, making high levels of CD44 a marker for activated and memory T cells 57. As immune cells have to be activated to bind Fl-HA, perhaps it is not surprising that the functional consequences of HA binding were not revealed until an immune response was initiated.
Factors that induce HA binding in primary, ex vivo leukocytes are summarized in Table 2.
Data is restricted to primary ex vivo immune cells and doesn't include data from cell lines. HA binding is measured using soluble Fl-HA and flow cytometry. Dendritic cells express HA on their surface as detected by Pep-172.
Stimulation of human peripheral blood monocytes with LPS, IFN , IL-1, TNFα, and GM-CSF all upregulate CD44 expression and induce HA binding 39,58, although TNFα produced in response to IL-1 and GM-CSF was responsible for the induction of HA binding in these cases. IL-4 and IL-13 inhibited TNFα-induced HA binding, and inhibition of N-glycosylation and chondroitin sulfate addition both prevented the IL-4 induced downregulation of HA binding 40. Although IL-10 is considered an anti-inflammatory cytokine, it also induced HA binding in human monocytes 40.
Neutrophils do not bind detectable levels of soluble Fl-HA following exposure to inflammatory stimuli and do not exhibit HA dependent rolling in vitro 59, yet the absence of CD44 or HA reduced neutrophil adherence and emigration in a chemokine-induced model of leukocyte recruitment 59. Neutrophil adhesion was also reduced at liver sinusoids in the CD44 null mouse upon LPS-induced hepatic injury 47. This apparent discrepancy may be explained if HA binding is too low to be detected by soluble Fl-HA or if HA binding in vivo is different from binding soluble Fl-HA in vitro. In support of the former, effector Th1 and Th2 cells that have very little detectable Fl-HA binding can roll in an HA-dependent manner 60. In support of the latter, two studies have shown that CD44 expressing cells bind more HA when other HA binding proteins, such as SHAP and TSG-6, are present 43,44.
An increase in CD44 expression and a transient induction in HA binding were observed in a percentage of both CD4+ and CD8+ T cell populations in vivo upon activation of an immune response 61,62. However, is not clear why HA binding is only induced in a percentage of activated T cells or why the binding is transient. Although all the CD8+ effector cytotoxic T cells were present in the HA binding population, neither blocking by HA or CD44 mAbs prevented cytolysis 61. However, other studies with human cytotoxic T cells, NK cells and NKT cells showed that CD44 crosslinking can enhance their effector function 63-65, and data from CD44 and LFA-1 double null mice show reduced conjugate formation in IL2-activated NK cells 66. HA binding also marks the most functionally active T regulatory cells 67 and high molecular mass HA can enhance the suppressive ability of these cells 68.
Antibody cross-linking of CD44 provides co-stimulatory activity during T cell activation 8, however, only one study with human peripheral T cells has shown that HA enhances CD3-mediated T cell activation 69. HA binding by CD44 may help mediate the interaction between dendritic cells and T cells 70,71 as HA is synthesized and expressed by dendritic cells 72 and reduced conjugate formation is observed in vitro when CD44 null T cells are mixed with mature dendritic cells 70,71. However, since naïve T cells do not bind detectable levels of Fl-HA, it is not clear to what extent this interaction is important for naïve T cell activation. HA and HA oligosaccharides can also induce dendritic cell maturation, but this occurs in a CD44-independent, toll-like receptor-4 (TLR-4)-dependent manner 73. To date, the best defined role for CD44 on activated T cells is its ability to mediate rolling on HA and on activated endothelial cells under flow conditions 74. This is discussed in more detail in the next section.
The function of CD44 in the immune response began to emerge with the investigation of mouse models of disease using either CD44 antibodies or CD44 null mice (reviewed in 75,76). Each approach has its limitations, with the CD44 mAb, IM7, able to induce rapid depletion of neutrophils from the circulation 77, while possible compensation effects may occur in the CD44 null mice 56. Nevertheless, both approaches have provided valuable insights into the in vivo function of CD44 and HA during an immune response. While the majority of evidence indicates a pro-inflammatory role for CD44 59,62, some disease models reveal a role for CD44 in the resolution of inflammation 78. Here we describe the functions of CD44 in the development and termination of an inflammatory immune response. These are summarized in Table 3.
Leukocyte extravasation to inflammatory sites involves a series of discrete steps: tethering and rolling, adhesion, diapedesis and migration (reviewed in 79-82). Firstly, leukocytes are captured from the blood by an initial tethering and rolling interaction. This reduces the velocity of the cell, allowing a greater opportunity for additional interactions with immobilized chemokines on the endothelial cell, which together with the rolling interaction, signal to upregulate integrin function. This leads to firm adhesion and cell arrest, followed by diapedesis and migration into the inflamed tissue. Selectins and their ligands, as well as CD44 and HA have been implicated in the initial rolling interaction leading to integrin-mediated adhesion (reviewed in 83,84 and see Fig. 2). While selectin expression on endothelial cells can be induced within hours of an inflammatory stimulus, and integrins activated by chemokine signals within minutes, the induction of HA binding by CD44 on T cells and monocytes is typically a slower process, requiring protein synthesis and up to three days to achieve maximal HA binding on the majority of cells. Although this slower upregulation may make some sense for T cells, which are first activated in the lymph node to proliferate prior to becoming effector T cells and migrating to the site of infection, the significance of the slower kinetics in monocytes is not understood. One would predict that optimal HA binding for monocytes would occur in the tissue, not in the blood.
T lymphocyte rolling on endothelium can be mediated by CD44. The interaction between CD44 on T cells and hyaluronan on the surface of the endothelium is sufficient for the initial "rolling" interaction, which is required for the extravasation to tissues. This rolling interaction, together with chemokines ( ) facilitates firm adhesion, mediated by the integrins ( ) and their counter receptors ( ) on endothelial cells which then leads to diapedesis and migration to the site of inflammation and infection.
The mechanisms and molecules used to recruit leukocytes to inflammatory sites differ depending on the organ and the leukocyte being recruited 85,86. In some organs, there is evidence to suggest that CD44 and HA can exert a pro-inflammatory role by facilitating leukocyte recruitment to inflammatory sites, thereby promoting chronic inflammatory disease.
Siegelman's group has contributed significantly to this area, making the initial key observation that T cells can roll on endothelial cells in a CD44- and HA-dependent manner 74,87. Inflammatory agents upregulate the expression of CD44 and HA on the surface of microvascular endothelial cells and induce HA binding, thereby allowing endothelial cell CD44 to present HA to the T cell and mediate CD44 dependent rolling 42,88. This and previous data 14 led to the proposal of a "sandwich" interaction model: CD44-HA-CD44 (see Fig. 3).
The "sandwich" model. A CD44:HA:CD44 mediated cell:cell interaction. Here CD44 expressed on activated T cells binds to hyaluronan presented on the surface of endothelial cells by CD44. The level of hyaluronan expressed by endothelial cells is increased in inflammatory conditions.
Siegelman's group then went on to demonstrate an effect of CD44 and HA in the in vivo extravasation of superantigen activated T cells to the peritoneum 62. CD44 was also required for subsequent firm adhesion mediated by the α4β1 integrin (VLA-4) and this required the cytoplasmic domain of CD44 89. This work implied that activated T cells could roll on HA induced on inflamed endothelial cells to facilitate extravasation to the inflamed site. Kubes' group was the first to show CD44- and HA-dependent rolling and adherence in vivo with Th1 and Th2 cells in a mouse model of TNFα-activated endothelium 60. Consistent with the sandwich model, CD44 was required on both the endothelial cell and the T cell.
A role for CD44 and HA in leukocyte extravasation had also been suggested by analysis of CD44 null mice and CD44 antibody studies in several models of chronic inflammatory diseases (reviewed in 75). The CD44 mAb, IM7 does not bind directly to the HA binding site of CD44 90, yet this antibody has dramatic effects in vivo. Given the recent finding that in vivo administration of IM7 can lead to leukocyte depletion 77, one should be cautious in the interpretation of studies using this antibody. However, in some mouse models of chronic inflammation, data from CD44 null mice and CD44 antibody studies both support a pro-inflammatory function for CD44 in T cell recruitment to sites of chronic inflammation (see Table 4). Early studies from Mikecz and colleagues identified an inhibitory function for IM7 in leukocyte infiltration in a mouse model of rheumatoid arthritis 91 and further studies in a mouse model of collagen-induced arthritis showed that CD44 null mice had significant reductions in both the incidence and severity of the disease 92. Using adoptive transfer to analyze T cell trafficking patterns, it was found that CD44 negative T cells had delayed entry into the joints of arthritic mice, suggesting that CD44 was affecting arthritis by facilitating leukocyte traffic to the site of inflammation 55,92.
In models of experimental autoimmune encephalomyelitis (EAE), administration of antibodies against CD44 and the VLA-4 integrin reduced T cell trafficking to the spinal cord 93 and brain 94 and prevented the development of EAE. Interestingly, as CD44-dependent extravasation to superantigen-inflamed peritoneum requires firm adhesion mediated by VLA-4 89,95, it raises the possibility that a cooperative interaction between CD44 and VLA-4 may be involved in T cell recruitment to the central nervous system.
CD44 may also be important for the recruitment of other leukocytes to sites of inflammation 59,96,97. Neutrophil accumulation in the tissues is reduced in CD44 null mice in a chemokine model of recruitment 59 and within liver sinusoids of endotoxemic mice 47. Reduced recruitment of CD44 null macrophages to atherosclerotic lesions 96 and into inflamed lungs following LPS inhalation 98 has also been observed, and HA can recruit macrophages to the site of damage 99. However, a defect in the recruitment of neutrophils or macrophages is not observed in CD44 null mice during bleomycin-induced lung inflammation 78 and a persistent increased neutrophil recruitment occurs in the inflamed lungs of CD44 null mice after Mycobacterium tuberculosis infection 100 or upon Escherichia coli-induced pneumonia 101.
How CD44 and HA influence neutrophil recruitment to inflammatory sites may differ from that of T cells. Unlike T cells, neutrophils and macrophages are activated in the tissues, and unstimulated neutrophils and monocytes present in the blood do not bind significant levels of soluble Fl-HA, suggesting that CD44 and HA may not be involved in the initial recruitment. Besides a defect in adhesion, migration deficiencies in CD44 null neutrophils 59,102 and macrophages 98 have been reported in vitro, and for macrophages 98 but not neutrophils 59 in vivo. Thus a defect in migration may contribute to reduced recruitment. Other possibilities are that leukocyte CD44 is required for firm adhesion, perhaps via its effect on VLA-4, or perhaps endothelial cell CD44 helps immobilize chemokines, which trigger firm adhesion and lead to subsequent migration. Proteoglycan-modified CD44 or proteoglycans bound to HA on endothelial cells may immobilize chemokines, thereby promoting integrin activation and leukocyte adhesion 103. MIP-1β for example, can bind to endothelial cells and to the proteoglycan form of CD44 and this promotes T cell adhesion 104.
While most chronic inflammatory models of disease indicate a pro-inflammatory role for CD44 and HA, most models of acute inflammation involving tissue injury indicate a role for CD44 in the resolution of inflammation (see Table 4).
In a bleomycin-induced lung injury model of acute inflammation in which mice normally recover, 75% of CD44 null mice die within 2 weeks 78. The increase in death was attributed to a failure to resolve the inflammation, characterized by reduced activation of transforming growth factor-β1 (TGF-β1), impaired removal of apoptotic neutrophils, and accumulation of both high molecular mass and fragmented hyaluronan. Active TGF-β1 is crucial for downregulating the immune response and promoting tissue repair in many experimental systems 105, including lung injury 106. TGF-β1 is secreted in a latent form that is activated by proteolysis 107. In keratinocytes, MMP-9 localization to the cell surface is CD44 dependent and can lead to TGF-β1 activation 108, raising the possibility that CD44 may help promote TGF-β1 activation. Indeed, CD44 promoted TGF-β1 activation and directional migration in fibroblasts 109, both of which promote wound healing.
HA can be cleaved by hyaluronidases and reactive oxygen/nitrogen species, and this, combined with increased hyaluronan synthesis, leads to the accumulation of HA and HA fragments at inflammatory sites (reviewed in 110,111). The majority of HA is normally turned over in the tissues by removal via the lymphatic system. However, local turnover of HA can account for over 25% of HA degradation 112. CD44 expression by alveolar macrophages has been implicated in the local turnover of HA in the lungs 113,114. Consistent with this, HA accumulation in the lungs of CD44 null mice treated with bleomycin was reduced in chimeric CD44 null mice reconstituted with CD44+ bone marrow 78. As fragmented HA can activate cells through TLR-2 and -4 115, these studies suggest an important role for CD44 in the removal of inflammatory HA fragments and restoration of HA homeostasis. CD44 can also negatively regulate TLR-4 signaling 116,117, suggesting another means to down-regulate the inflammatory response.
Although CD44 has been suggested to be a phagocytic receptor for CD44 antibody coated beads 118, subsequent work suggested that CD44 mAbs have an indirect effect on phagocytosis by activating the complement receptor-3 driven phagocytic pathway 119. Other work has shown that incubating macrophages with an anti-CD44 mAb can increase their phagocytosis of apoptotic neutrophils 120, raising the possibility that CD44 may indirectly affect the efficient removal of apoptotic neutrophils, a necessary step in the resolution of an inflammatory response.
There is also evidence that CD44 is involved in activation-induced cell death (AICD). AICD is responsible for the removal of activated T cells and restoration of immune homeostasis 121. High molecular mass HA can induce AICD in a subpopulation of activated T cells in vitro 41. In vivo, the lack of CD44-dependent AICD has been associated with increased severity of a delayed type hypersensitivity response and concanavalin A-induced hepatitis in CD44-deficient mice 122,123. The absence of CD44 also exacerbates the lymphoproliferative symptoms found in mice that do not express Fas, the receptor primarily responsible for AICD 124. Thus, despite the prominent role that Fas plays in AICD and a report that CD44 crosslinking can increase surface expression of Fas in primary human T cells 125, data from primary mouse T cells 124 and human Jurkat T cells 41 shows that CD44-mediated AICD occurs by a Fas-independent mechanism.
HA can increase AICD in CD44+, but not CD44 negative T cells activated in vitro 41. In addition, the extent of HA-induced cell death in human Jurkat T cells is dependent upon the extent of HA binding: high HA binding cells are more susceptible to death than low HA binding cells and high but not low molecular mass HA, enhances AICD. This suggests that the strength or degree of HA binding by CD44 can affect the biological outcome. Furthermore, as high molecular mass HA is a characteristic of normal, undamaged, uninfected tissue, it makes sense that it can trigger the death of activated T cells to promote tissue homeostasis.
The interaction between CD44 and its multivalent ligand HA is unique in that it can range from a low affinity, low avidity interaction to a high affinity, high avidity interaction. There is some evidence to suggest that the strength of the interaction may affect the biological outcome. For example, Th1 and Th2 cells with low HA binding avidity can roll on HA whereas high HA binding on activated T cells can trigger cell death. Although we cannot exclude a role for CD44 and HA in immune cell development and homeostasis, most of the evidence to date has pointed towards the importance of the interaction in regulating the immune response. This is supported by the fact that the majority of immune cells don't bind HA until they are activated by an antigenic or inflammatory agent. There is evidence that CD44 and HA can play a pro-inflammatory role in facilitating leukocyte recruitment to inflammatory sites, although the mechanism may differ between T cells and neutrophils. The extent to which CD44 and HA are important in this process may be organ and disease specific. CD44 can also play a role in the resolution of inflammation and restoration of HA and tissue homeostasis. CD44 on activated macrophages may assist in the removal of inflammatory HA fragments, and the binding of high molecular mass HA to activated T cells may facilitate their removal and the restoration of tissue homeostasis.
Our research on CD44 was supported by the Heart and Stroke Foundation of B. C. and Yukon, the Canadian Institutes of Health Research and the National Science and Engineering Council of Canada. Brian Ruffell was funded by a graduate fellowship from the Heart and Stroke Foundation of Canada.
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Q: Unable to convert Javascript code to JQuery Like some of the beginners, i have been trying to learn JQuery by transforming the javascript code to jquery.
Now here is a part of code which use HTML Canvas and allows user to draw and delete the content in Canvas using Jquery:
function init() {
canvas = $('#can');
ctx = canvas.getContext("2d");
w = canvas.width;
h = canvas.height;

canvas.addEventListener("mousemove", function (e) {
    findxy('move', e)
}, false);
canvas.addEventListener("mousedown", function (e) {
    findxy('down', e)
}, false);
canvas.addEventListener("mouseup", function (e) {
    findxy('up', e)
}, false);
canvas.addEventListener("mouseout", function (e) {
    findxy('out', e)
}, false);}

This code was initially in Javascript, i have just changed the Selectors to use it as Jquery. But I am getting the error as in the 3rd line of this code as:
Uncaught TypeError: undefined is not a function
Could you please guide why i am getting this. Thanks

A: getContext is not a jQuery property, therefore you need to access the underlying DOM element and call it on that:
ctx = canvas[0].getContext("2d");

Also, jQuery convention is to use the $ prefix on a variable containing a jQuery object. width and height are also methods, so should have trailing brackets. Finally, if you want to use jQuery, use it to attach your events too:
function init() {
    var $canvas = $('#can');
    var ctx = $canvas[0].getContext("2d");
    var w = $canvas.width();
    var h = $canvas.height();

    $canvas
        .on("mousemove", function(e) { findxy('move', e); })
        .on("mousedown", function(e) { findxy('down', e); })
        .on("mouseup", function(e) { findxy('up', e); })
        .on("mouseout", function(e) { findxy('out', e); });
}

A: Try using canvas = $('#can')[0];

A: This is because any jQuery selectors will return a jQuery object not a JS element instance. If the selector returns only 1 element, it is always in the 0 index of the returned object:
canvas = $('#can')[0];
ctx = canvas.getContext("2d");

redpajama_set_name: RedPajamaStackExchange


text:
Ksenija Đurović
Ksenija is a cultural worker based in Belgrade focusing on organisation and production models in the independent art scene. Holds a BA from the Faculty of Dramatic Arts, Belgrade where she was also undergraduate PA (2008-2010).
Since 2011 has worked as project coordinator with Station Service for contemporary dance, an organisation working adamantly for the development of this field in Serbia. Coordinated several projects in Station which involved developing new practices for work with children, mentoring and other educational programmes for critical writing, residencies, international cooperation and coproduction, advocacy etc. Ksenija is active in Magacin, a self-governed, independent cultural centre which is (still) illegal after attempts by the City of Belgrade to close it.
Ksenija produces independently in both contemporary performing and visual arts. She works with the younger generation of choreographers like Dragana Bulut, Igor Koruga, Ana Dubljević, Jovana Rakić and Marko Milić. She has produced an international group exhibition Invisible Violence with curator Zoran Erić (MoCAB) and an exhibition by Katarina Popović (Remont).
In November 2018 she co-founded BRINA with curator Mirjana Dragosavljević in hopes to develop their own feminist, inclusive, critical work in visual/performing arts starting with their exhibition in the Belgrade Cultural Centre - Migrations (July 2019).
Ksenija went through different informal educational programmes like Socialism studies (CPE, Belgrade), is currently attending Studies of the Commons (zajedničko.org) and is a former fellow of Akademie Schloss Solitude, Stuttgart. Since October 2018 she is the programme producer of Bitef, one of the region's oldest theatre festivals.
Ksenija is a Managing Board member of the Association Independent Cultural Scene of Serbia where she works on projects involving participative governance, monitoring of public spending and decentralisation of culture in Serbia.
How to make open, inclusive and flexible governance models?

redpajama_set_name: RedPajamaCommonCrawl


WILSON, N.C. — Furry or feathered, large or small, pets and their families are invited to join the Barton College community for a "Blessing of the Animals" service to be held on Sunday, Oct. 13, at 3 p.m. There is no charge, and the surrounding community is invited to attend the service.
Barton's "Blessing of the Animals" service, to be led by the Reverend Jamie Eubanks, will last approximately 20 minutes and will take place on center campus. All pets are welcomed, but please consider the wisdom of bringing aggressive animals. Please leash all pets as appropriate. Large animals also are welcomed, but they must be led onto center campus and not ridden. If you are unable to bring your pet, you may bring a photograph of your pet. Clean-up supplies and water will be available.
For additional information or questions, please contact Rev. Eubanks at 252-399-6368 or email: jceubanks@barton.edu.


text:
L'Ur-Paarl nach Klosterart, che letteralmente sta per "l'originario pane di segale doppio alla maniera del convento", è la variante più antica del tipico Vinschger Paarl della Val Venosta.

Descrizione
La forma tradizionale è una specie di "otto" schiacciato, ottenuta unendo due pani rotondi e piatti. Per questo si chiama paarl, che significa coppia. La dimensione è variabile: il diametro va dai 10 ai 30 centimetri e lo spessore da 2 a 3 centimetri.

I depositari della ricetta madre sono i monaci benedettini dell'Abbazia di Monte Maria, sopra Burgusio nel comune di Malles Venosta. La riscoperta dell'Ur-Paarl è avvenuta grazie al recupero dell'antica ricetta custodita dall'ultimo monaco fornaio, fratel Alois Zöschg, ripresa da un gruppo di fornai dell'Alta Val Venosta, i quali producono i piccoli pani con farina di segale, farina di farro e lievito madre.

Secondo la tradizione l'Ur-Paarl veniva preparato in occasione di matrimoni, per cui la sua forma particolare significherebbe l'unione degli sposi.

Ognuno personalizza la ricetta aggiungendo dosi variabili di semi di finocchio, cumino selvatico e trigonella caerulea, erbe raccolte sui pascoli della valle.

Con l'aggiunta di albicocche, pere e frutti di bosco diventa un pane dolce: lo Früchtebrot.

Note

Voci correlate

 Vinschger Paarl

Prodotti tipici dell'Alto Adige
Pane italiano

redpajama_set_name: RedPajamaWikipedia


text:
Police seek clues in New York blast that hurt 29; no terrorism links found
No international group had claimed responsibility "but it is very, very early in the investigation," the state governor told reporters.
Sep 18, 2016, 22:55 PM IST
New York: New York Governor Andrew Cuomo said on Sunday there was still no indication that an explosion that injured 29 people in Manhattan on Saturday was linked to international terrorism, while investigators scoured the scene of the blast.
FBI investigators were to examine remnants of the bomb plus an unexploded device found four blocks away and another device that exploded about 80 miles (130 km) away in New Jersey on Saturday to see if they were connected, Cuomo said.
No international group had claimed responsibility "but it is very, very early in the investigation," the state governor told reporters, reiterating what New York City Mayor Bill de Blasio had said hours after the blast.
"A bomb exploding in New York is obviously an act of terrorism, but it`s not linked to international terrorism. In other words, we find no ISIS connection, et cetera," Cuomo said, referring to the Islamic State.
With world leaders due to arrive in the city ahead of the U.N. General assembly, some 1,000 additional state police and National Guard officers were being sent to the city to patrol transportation hubs as a precaution, Cuomo said.
Heads of state and government begin six days of speeches in New York on Tuesday, with some dignitaries arriving early for preliminary events.
A sweep of the neighborhood following the blast had turned up another device four blocks away consisting of a pressure cooker with wires attached to it and connected to a cell phone.
Pressure cooker bombs were used in the 2013 Boston Marathon bombing that killed three people and wounded more than 260.
The New Jersey explosion came from a pipe bomb, officials said. A U.S. official familiar with information circulating inside the government said the motive remained unknown and insufficient evidence had been gathered to link the two New York bombs. There was no evidence to connect them to the New Jersey blast, said the source, who spoke on condition of anonymity.
"From what we know now, and it`s limited, almost anybody could have fabricated these bombs and used cellphones as timed detonators," said another U.S. official familiar with the inquiry. "There are instructions all over the internet, and the crudity, positioning, and relative ineffectiveness of these does not suggest that a more sophisticated group played any role in this."
The U.S. Federal Bureau of Investigation (FBI) was taking evidence from all three devices to its special crime laboratory in Quantico, Virginia, said Cuomo, adding that he had been briefed by "all the relevant agencies."
"We will find who planted these explosives and they will be punished. ... We will not allow these type of people and these type of threats to disrupt our life in New York," Cuomo said.
Most of the injured were treated for minor injuries including shrapnel wounds and released, though police listed one injury as serious.
"When you see the damage, I think we were fortunate that there were no fatalities," Cuomo said.
New York police, the FBI and the U.S. Bureau of Alcohol, Tobacco, Firearms and Explosives converged on the site for their first daylight view of the explosion, closing several blocks of West 23rd Street to traffic.
Investigators cordoned off the area with yellow crime scene tape and placed dozens of numbered evidence markers on the ground. Neighborhood residents who could show identification were allowed back into their homes.
The blast, described by one neighbor as "deafening," reverberated through the Chelsea district of Manhattan around 8:30 p.m. (0030 GMT Sunday) on 23rd Street, a block of mostly commercial spaces at street level with residential floors above. The Flatiron Building, a New York landmark, is about 500 yards (meters) away.
It happened outside the Associated Blind Housing facility at 135 W. 23rd Street which provides housing, training and other services for the blind. The same block is home to a Roman Catholic Church with a neoclassical facade, a Basque restaurant, a showroom for exercise cycles and a small hotel, among other businesses.
The explosion quickly became an issue in the presidential race. Republican candidate Donald Trump told an audience, "We better get very tough, folks." Democratic rival Hillary Clinton said in a statement she had been briefed but would wait until she had more information before commenting further.
US policeNew YorkNew York Governor Andrew CuomoManhattan
Russian holds parliament vote with little threat for Putin


text:
Property scam targets stars
SPORTING superstars have been unwittingly sucked into suspected property scams that are linked to a convicted conman who disappeared in a mysterious plane crash earlier this year.
By Ted Jeory
PUBLISHED: 00:00, Sun, Dec 14, 2008
Footballer Gareth Barry was an Ocean View investor
Football great John Barnes, tennis legend Pat Cash, cricket's Darren Gough and rugby's Martin Corry and Martin Offiah are just some of the high profile figures who were promised luxury apartments if their names could be used to promote overseas developments that many fear will never be built.
The promises were made by a Spanish company, which was closely involved with Sean Woodhall, a 43-year-old fraudster who disappeared after a light aircraft carrying him and other debt-ridden businessmen crashed in a Brazilian forest in May.
The sportsmen have been offered holiday homes at the proposed "five-star" resort in Tafedna Bay, Morocco, which does not have planning permission, where not a spade has been turned and yet on which sales agents have already been taking cash from British customers.
Former England rugby captain Corry this week cancelled his involvement in the project after police warned him about the potential fraud.
"He no longer wants anything to do with it," his spokesman said.
Pat Cash is also reconsidering his contract.
In another closely related suspected scam, a group of well-known footballers, including one ex-England international, are preparing to sue Midlands-based Ocean View Properties, which the Sunday Express exposed last week as having left hundreds of customers millions out of pocket.
The company is also believed to have conned two serving police officers.
Ocean View, which has debts of more than £100million, this week took down its website "for maintenance".
Both police and Government fraud agencies are now investigating the company and a multimillion-pound black hole.
The suspected swindles involve four central characters and their cross-border labyrinth of companies, which lured people to invest in "off-plan" holiday-homes in Spain, Morocco and the Dominican Republic.
Buy-to-let millionaire Colin Thomas is the owner of Ocean View Properties; Woodhall, whose body has never been recovered, was Mr Thomas's dealmaker in Spain; Adam Sargent was Mr Thomas's expert salesman in the sports world; and Spaniard Ricardo Miranda was the supposed developer in all three countries.
Mr Thomas formed Ocean View in 2001, the year that Woodhall moved to Spain after being convicted for a franchise fraud in Birmingham.
Woodhall found sites in the Costa del Sol, while in Britain Mr Thomas created a network of franchise agents to sell off-plan apartments.
These agents were part of Ocean View's "one-stop shop" property service, which allowed it to control cashflows and direct customers to its own recommended mortgage brokers, travel agents and Spanish conveyancing lawyers-a practice criticised by the buy-to-let industry's own trade body.
Among these agents was Mr Sargent, a financial adviser to star footballers.
He helped Ocean View sponsor Aston Villa, West Bromwich Albion, and Leicester City.
They told cash rich footballers there were guaranteed rental yields and high capital profits abroad.
Among the many footballers to invest in discount deals were Villa's Gareth Barry, Newcastle United's Alan Smith and Shola Ameobi, and ex-Liverpool midfielder Steve Staunton.
In return they allowed their names to be used on promotional material.
Similar deals were also struck with England cricketers Duggie Brown and Paul Nixon, who still advertise their involvement with Ocean View on the Professional Cricketers' Association website.
Customers would exchange contracts with a Spanish developer and hand over typical deposits of £70,000 to Ocean View in Britain.
Mr Thomas said he would then transfer the bulk of the deposit to the developer.
Many of the properties were built, but some were sub-standard, according to customers.
However, cracks surfaced when the Costa del Sol property market collapsed amid a widespread corruption scandal in 2006.
It then emerged that Ocean View and Mr Miranda's development company, Sungolf, had been taking deposits for the proposed Estepona Beach and Country Club near Malaga, a site that did not have planning permission and which has yet to be built.
That was not allowed under Spanish law, legal sources have said.
They have also said that Ocean View's practice of charging customers Spanish VAT on the full purchase price rather than on just the initial deposit was also illegal.
Customers, many of them preparing legal cases, are now claiming they were deliberately duped.
Last week, Ocean View's owner Colin Thomas said because Sungolf had all the cash, it would be starting a refund scheme in February.
However, Mr Miranda's spokeswoman denied that was the case, claiming they had "proof" the money had already been "returned" to Ocean View.
Meanwhile, Ocean View has also been telling unhappy customers they can transfer their contracts to another of Mr Miranda's developments-Morocco's Tafedna Bay, a site where Mr Sargent has been touting for business through his new company, Zenith Overseas Investments.
The links between the businessmen can also be traced to Mr Miranda's Punta Perla development in the Dominican Republic, an empty site where Newcastle's Alan Smith has also invested.
Last week, Ocean View's Mr Thomas said he had no involvement there, but the Sunday Express has obtained a sales document, which states that cheques for deposits in Punta Perla should be sent to his company in Staffordshire.
Mr Thomas also said last week that he had severed relations with conman Woodhall in 2004.
However, Ocean View was acting as agents for Woodhall's now-defunct Punta Perla Caribbean company from late 2005.
Two weeks ago Woodhall's other company, Worldwide Destinations, was expelled from the industry's trade body, the Association of International Property Professionals, for allegedly selling non-existent mortgages in Egypt.
Mr Thomas, Mr Sargent, and Mr Miranda have all strongly denied any wrongdoing.
Mr Sargent said: "I took on an Ocean View franchise for five years and recommended it as a pension replacement and pension supplement scheme to my clients.
"The idea was to get people to buy properties, then get them built, and put them into a rental programme with its own travel arm.
"Generally, they've done that to a degree. The travel arm wasn't as successful as everybody had hoped, but we've managed to put people into a long term rental contract and I've been a lot more hands on than most in the sense that I've tried to manage the clients expectations."
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text:
Daugård er en by i Østjylland med , beliggende nær Vejle i Daugård Sogn. Daugård har foruden dens stationsby givet navn til Daugård Mark og Daugårdstrand i dens lille opland. Postnummeret 8721 dækker også Ørum Sogn, som indgår i et fælles områdesamarbejde. Daugård hører til Hedensted Kommune og dermed Region Midtjylland.

Byen nævnes første gang i historiebøgerne i 1399 under navnet Daghægord .
I Daugård ligger den større gård Williamsborg, Daugård Kirke, en multihal med idrætsforening, en købmand med postbutik, og nogle erhvervsbygninger. Desuden findes der en folkeskole samt gymnasiet Vejlefjordskolen syd for byen. Byområdet er under udvidelse og vokser nordpå mod Hedensted.

Daugård er desuden hjemsted for flere berømte danskere. Såvel rapperen Tue Track som den tidligere danske landsholdsspiller Thomas Gravesen stammer fra byen. Fodboldtræneren Peter Sørensen er bosat i byen.

Eksterne henvisninger
 Lokalrådets hjemmeside med oversigt over foreninger mv.
 Radioprogram fra DR fra Daugård

Kilder

Byer i Hedensted Kommune


text:
'Instinct': Marc Webb To Direct CBS Drama Pilot Starring Alan Cumming
Nellie Andreeva
Co-Editor-in-Chief, TV
@DeadlineNellie
More Stories By Nellie
'SpongeBob SquarePants' Renewed For Season 13 By Nickelodeon On Heels Of Strong Ratings For Anniversary Special
'Nailed It!' Breaks Into Emmys With Nom For Outstanding Competition Program As 'Project Runway' Streak Ends
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Marc Webb is set to direct and executive produce Instinct, CBS' drama pilot starring and executive produced by Alan Cumming.
Written by Michael Rauch based on the upcoming book by James Patterson, Instinct (fka Killer Instinct and Dr. Death), centers on a former CIA operative (Cumming) who has since built a "normal" life as a gifted professor and writer but is pulled back into his old life when the NYPD needs his help to stop a serial killer on the loose.
Rauch, Cumming, Patterson and Webb executive produce alongside Bill Robinson and Leopoldo Gout of James Patterson Entertainment, as well as Kurtzman and Heather Kadin of Secret Hideout. CBS Television Studios — where Rauch and Secret Hideout are under overall deals and James Patterson Entertainment has a first-look deal — is the studio.
For Webb, the gig stems from his overall deal at CBS Studios. He executive produces the studio's CW dramedy series Crazy Ex-Girlfriend. Webb has directed three pilots to date, which all have gone to series, Crazy Ex-Girlfriend, CBS/CBS Studios' Limitless and Fox/20th TV's Lone Star. The 500 Days of Summer and Amazing Spider-Man director is repped by CAA.
This article was printed from	https://deadline.com/2017/02/instinct-marc-webb-direct-cbs-pilot-alan-cumming-1201905857/