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Home » Celebrities » Emilia Clarke pens an emotional goodbye to Game of Thrones
Emilia Clarke pens an emotional goodbye to Game of Thrones
19/05/2019 Comments Off on Emilia Clarke pens an emotional goodbye to Game of Thrones
'The Mother of Dragons has taken up my whole heart': Emilia Clarke pays tribute to Daenerys Targaryen and thanks her 'magical fans' as she pens a VERY emotional goodbye to Game Of Thrones ahead of finale
Emilia Clarke has penned an emotional tribute to Game of Thrones and its 'magical fans' as she prepares to say goodbye to the series ahead of its finale on Sunday.
The actress, 32, has appeared as the fierce Mother of Dragons Daenerys Targaryen in the HBO fantasy drama since it first hit the small screen in 2011.
After eight seasons, and a deadly penultimate episode, Game of Thrones will finally unveil who will reign victorious in Westeros when the show concludes on Sunday.
Emotional: Emilia Clarke, 32, has penned an emotional tribute to Game of Thrones and its 'magical fans' as she prepares to say goodbye to the series ahead of its finale on Sunday
Taking to her Instagram, Emilia shared a moving tribute to Daenerys who has 'taken up the whole of my heart' alongside a series of behind the scenes shots.
She wrote: 'Finding the words to write this post has left me overwhelmed with how much I want to say but how small words feel in comparison to what this show and Dany have meant to me.
'The mother of dragons chapter has taken up the whole of my adult life. This woman has taken up the whole of my heart.
Co-stars: Taking to her Instagram, Emilia shared a moving tribute to Daenerys who has 'taken up the whole of my heart' alongside a series of behind the scenes shots
Conclusion: After eight seasons, and a deadly penultimate episode, Game of Thrones will finally unveil who will reign victorious in Westeros when the show concludes on Sunday
'I've sweated in the blaze of dragon fire, shed many tears at those who left our family early, and wrung my brain dry trying to do Khaleesi and the masterful words, actions (and names) I was given, justice.'
The Me Before You star explained her time on the show has 'shaped [her] as a woman, as an actor and as a human being'.
Emilia then said she wished her father, who passed away from cancer in July 2016, had been here to see 'how far we've flown'.
Fierce: The actress has appeared as the fierce Mother of Dragons Daenerys Targaryen in the HBO fantasy drama since it first hit the small screen in 2011
She wrote: 'Game of Thrones has shaped me as a woman, as an actor and as a human being. I just wish my darling dad was here now to see how far we've flown.
'But to you, dear kind magical fans, I owe you so much thanks, for your steady gaze at what we've made and what I've done with a character that was already in the hearts of many before I slipped on the platinum wig of dreams. Without you there is no us.'
Emilia ended her tribute with a poignant nod to a line said by Jon Snow (Kit Harington) when he walked away from his command of the Night's Watch.
She wrote: 'Game of Thrones has shaped me as a woman, as an actor and as a human being. I just wish my darling dad was here now to see how far we've flown'
Poignant: Emilia ended her tribute with a poignant nod to a line said by Jon Snow (Kit Harington) when he walked away from his command of the Night's Watch
She said: 'And now our watch has ended.'
Her friends and co-stars have since commented on the post to show the actress support.
John Bradley, who portrays Samwell Tarly in the HBO fantasy drama, said: 'Beautiful'.
Her ex-beau, film director Charlie McDowell also commented on the tribute. He said: 'As you continue to fly, he flies beside you. Congratulations E.'
Support: Her friends and co-stars have since commented on the post to show the actress, who has appeared in the show since its first series aired in 2011, support (Pictured with Iain Glen as Jorah Mormont)
Pals: John Bradley, who portrays Samwell Tarly in the HBO fantasy drama, deemed the message 'Beautiful'
Crazy Rich Asians star Henry Golding said: 'You madam, are a supremely talented bean'.
Her tribute comes ahead of the much-anticipated finale of the epic, which will air on Sunday night.
Emilia recently revealed she suffered and survived two brain aneurysms while filming the fantasy drama, the second of which nearly killed her.
The actress, who first fell ill when she was just 24, made an appearance on CBS Sunday Morning in April where she recalled the dark place she went to after her surgery, and shared images from her hospital stay.
Horrendous: The Game Of Thrones star recently told of how she suffered and survived two brain aneurysms while filming the fantasy drama, the second of which nearly killed her
Hospitalised: The actress made an appearance on CBS Sunday Morning in April where she recalled the dark place she went to after her surgery, and shared images from her hospital stay
During the interview, Emilia also revealed what it was like to return to the set after her brain haemorrhage which happened after filming season one of the show.
She said that once she returned to shoot the second season she had no time to 'question her own mortality' because she was too busy playing a 'bad*** character who walked through fire'.
Emilia said: 'You go on the set, and you play a bad*** character, and you walk through fire, and you speak to hundreds of people, and you're being asked to be – to work as hard as you possibly can.
'And that became the thing that just saved me from considering my own mortality, yeah,' she said of working on the set while dealing with her first aneurysm.
Confession: During the interview, Emilia also revealed what it was like to return to the set after her brain haemorrhage which happened after filming season one of the show
The Solo star was quickly sent for an MRI after the shocking event, where they diagnosed her with a subarachnoid haemorrhage.
This type of hemorrhage is a life-threatening form of stroke, which is caused by bleeding around the brain and a third of patients die immediately or soon after suffering one.
Emilia said: 'In my worst moments, I wanted to pull the plug. I asked the medical staff to let me die. My job—my entire dream of what my life would be—centred on language, on communication. Without that, I was lost.'
Game Of Thrones' season eight finale will air on Sky Atlantic at 2am on Monday in the UK or 9pm Eastern time on Sunday May 19.
The end: Emilia's tribute comes ahead of the much-anticipated finale of the epic, which will air on Sunday night
'I just wish my darling dad was here to see how far we've flown' – Emilia Clarke says goodbye to Game of Thrones ahead of finale
JD McCrary Spills On How He Got His 'Lion King' Role!


The government is for the PEOPLE and the impacts of climate change on are real for all victims of hurricanes, droughts, fires. If we don't want 10x more countries to go the Syria route, we need to our tax dollars to at least make this national security threat visible at the front of the one agency accountable for it. We understand that profits will need to undergo transformation, but cowardice to not evolve should no longer be accepted. There are 1001 ways that this is a great time to be alive, in business, and able to read science and natural disaster facts: we can ACT, like China got its act together to deal with its own environment, forming dynasties around historic efforts to be ready. Let's not give the US "ostrich" reputation, holding on to finance as its only claim to fame on the international stage. Let's truly be innovators in a universe that leaves no forgiveness for risks ignored, and requires evolution for any actors that shall remain standing. I'm nowhere near ready to sit down.


You've arrived at our Mr Al's Get n Go operation conveniently located just west of Interstate 80. If you're traveling on Interstate 80, take Exit #102 and head west on Dewar Drive. We are just a block away on your right hand side. Going hunting or fishing? We've got licenses for both; as well as bait and tackle. Check out our selection of beer, wine or liquor in the liquor store.


I attached a few annotations to my model and tried to view them using google cardboard. The little numbers representing the annotation appears and I can hover and click on them but its just blank without the words I wrote on it. Thanks!
This used to work for me, then the problem you describe appeared and right now I'm not seeing my annotations in VR at all.
Setting annotations_visible to 1 has not helped.
Hello, thanks for reporting the issue.
It's a known issue and the fix should be deployed soon.
I fixed it again, it should be deployed soon.


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Tag: Band of Holy Joy
Fascinating Things : Issue 16
Videos of previously covered things have been released so here they are for the Band of Holy Joy single (Issue 11) and a teaser for the Pure Phase Ensemble album (Issue 14)
Fronted by singer Lou Reid, Lola in Slacks exude a sexy garage swagger wrapped in a eurocentric chanson motif. Described by their promo people as an ambitious, literate gang of Francophile bohemians, they originate in Glasgow.
The rest of the band comprises guitarists Brian McFie and Martin Stuart Taggart. keyboard player Villy Karagouni drummer Lesley McLaren and bassist Davy Irvin.
Their debut single 'Tramlines' is released on August 31st on Stereogram Recordings. it was recorded with award-winning producer Sandy Jones at Foundry Music Lab in Motherwell.
Reid's husky vocal tones, and rich lyrics and complimented by a tight early VU sound of ascending chords and crisp rhythms. It will be featured on next weeks Aural Delights. Very tasty.
Bristol in June 2016 sees the return of the Temples Festival which enters its triumphant third year, after two magnificent, sold-out events that brought patrons in contact with some of the most revered artists operating in the spheres of extreme and heavy music. From the international headl-iners (that have included Clutch, Converge, Earth, Electric Wizard, Neurosis, Pig Destroyer, SUNN O))) and Voivod) right down to the gritty native acts, there's a strong sense of cohesion, community and celebration at Temples that evidently goes down a treat with heavy music lovers.
Three days of unforgettable appearances and high volume antics in the heart of Bristol are already set in stone for the dates of June 2nd-5th and with lessons learned, eardrums repaired, and justified excitement, organisers have announced the following eighteen acts for the highly anticipated festival:
Organisers say that there is much more to be announced, including the three main-stage headliners as well as a vast array of bands from the international heavy underground. Tickets are available exclusively through http://www.templesfestival.co.uk, and day tickets will become available as individual day splits are announced.
"Inversum" is the forthcoming new full-length from Finnish psychedelic doom outfit, Dark Buddha Rising. Their fifth studio offering and first under the Neurot Recordings banner, Inversum is a two-track exercise in the blacker end of psychedelia, swallowing its listeners whole into a tense, brooding realm of dark atmospheres, harrowing mysticism, tormented howls and slow, ominous riffing. Considered the opening of the Third Cycle Of Dark Buddha Rising, the record was recorded, produced and mixed by the band in the bowels of the Wastement, the self-proclaimed "asylum of eternal feedback," and welcomes the addition of new Dark Buddha Rising recruits, V. Vatanen (guitar, vocals), J. Saarivuori (keyboards) and M. Neuman (vocals).
As a precursor to its release next month, here is an edited version of opening epic peice, "E S O."
Posted on August 11, 2015 August 12, 2015 by HalfEdgeMediaPosted in Gig News, Ramblings, Video, WebzineTagged Band of Holy Joy, Dark Buddah Rising, Lola In Slacks, Mark Gardener, Pure Phase Ensemble, Ride, Temples Festival. Leave a comment
Sounding a bit like Robert Forster singing with The Smiths Band of Holy Joy have a new download single "Isn't that just the life" out on Stereogram on August 10th. A video accompanies the download and there will be a select number of dates across the UK. An album follows in September.
I have been hand selected by The Working Man (lead by the hardest man in Blackburn) to listen to their new track "Big Bad John". They advise me that they are not going to bore me on how they 'merge different genres' and 'can't be put into any category' – they appreciate , like themselves, that I am very busy. A fascinating melange of differing things makes for a damn fine listen. It comes from a soon to be released album called "Lancashizer". You can catch them at the Bierkellar in that Manchester on July 30th.
Ancient River's new album 'Keeper of the Dawn' stems from band founder James Burreto's love of Italian horror films and the music of David Axelrod – some tracks are led by the vocals, others by the drums. The latest in a string of interesting albums the overall feel is airy psychedelia with space rock, shoegaze, and touches of Americana. Opening track "This is the Time" is a bit of a stunner.
Steve Hauschildt has announced his first new album since the late 2012 release of "Sequitur. Where All Is Fled" . It will be released via kranky on 25th September. Titled "Although Where All Is Fled" it sonically refers back to his earlier albums such as "Rapt for Liquid Minister" and "Tragedy & Geometry", but on detailed listening it slowly becomes apparent that it is also a divergence from those recordings.
"Both the artwork and the music on this new work were heavily inspired by surrealist landscape paintings, early alchemical emblems, and recurring visions Steve had from dreams. The result is a pristine series of cascading melodies, fantastical terrains of layered lattices, and overlapping patterns of synthesizers superimposed with orchestral instrumentation. Hidden in the crevices of the album are processed crowd sounds, re-sampled text-to-speech synthesis, piano, and animal noises which reveal themselves after repeated listens and blur together notions of artificial and natural sound. While slowly unfurling, each sound is given it's own place and space, never hurried, never cluttered."
Helen is a pop group from Oregon featuring members of Grouper and Eternal Tapestry. Liz Harris (vocals/lead guitar), Jed Bindeman (drums/tambourine), Scott Simmons (bass/guitar), and Helen (back up vocals). Originally started with the intention of being a thrash band, it turned into something else entirely. "The Original Faces" was recorded over a period of several years in Portland by the band members, their friends Nick, Chris and, largely, Justin Higgins. The record was written together, with some songs based on Liz and Scott's demos.
And finally for this edition of Fascinating Things
Rock Back for Nepal is the first in a 3-part series of disaster relief compilations being released by U.S. indie label Patetico Recordings. The label is partnering with nearly 60 independent artists and several small indie labels around the globe to make this happen. They have all come together in order to raise funds for victims of the recent catastrophic earthquakes in Nepal. All money raised will go directly to the relief efforts.
The compilations are being supported by several luminaries of the independent rock and shoegaze scene, among others. "Whenever Tom Lugo reaches out to his musical family, you know it's for a crucial and essential cause. The Rock Back for Nepal CD compilation is no exception," explains Dean Garcia of seminal shoegaze bands Curve and SPC ECO. "I'm proud to be part of this relief effort / fund raiser for anyone affected by the recent earthquake disaster in Nepal and if I can help or contribute in some way to aid support and assistance to the many displaced people, I will without question. Please support this cause and donate as generously as you can."
Perhaps one of the most renowned personalities in the shoegaze / noise rock spheres, Oliver Ackermann (A Place To Bury Strangers, Skywave) has also lent his support to the cause. "It is a horrible tragedy this destruction of culture and life. I feel lucky that I can use my craft and contribute a song to this fund to help them raise money for relief. If we can all do something to help and give relief for the people of Nepal we should do it now."
"It's great to be among this sparkling array of brilliant artists, taking action in support of a great cause," says Marc Joy of Welsh outfit Lights That Change. Alexx Kretov of Canadian-Ukrainian duo Ummagma is also hopeful."May the common freebie attitude fall to the wayside for this project. We really hope that people will support a higher cause by spending a few dollars and spreading this music far and wide."
All three volumes are being released at the same time, and will be available through Bandcamp and Amazon.com exclusively, both digitally and on CD.The album artwork and design is by Gardy Perez.
The concept of the Rock Back compilation series was born in 2011 when disaster hit Japan and Tom Lugo, owner of Patetico Recordings, acted on his empathy for charitable causes and non-profit organizations by reaching out to the community of artists in his network. They collectively put together a compilation and the profits were donated to relief efforts. The response was overwhelming, with over 100 artists from across the globe joining in the efforts. What was to be a single CD compilation grew into the 8-disc compilation Rock Back for Japan. The compilation series hit the top 10 Indie Rock releases on Amazon.com's chart for several weeks, with certain volumes holding the number 1 spot.
Posted on July 22, 2015 July 24, 2015 by HalfEdgeMediaPosted in Album Review, Artist Overview, Music Diary, New Releases, Ramblings, Short Reviews, WebzineTagged A Place To BUry Strangers, Ancient River, Band of Holy Joy, Curve, Helen, Lights That Change, Skywave, SPC ECO, Steve Hauschildt, The Working Man, Ummagma. 1 Comment

redpajama_set_name: RedPajamaCommonCrawl


I received an e-ARC of the book from NetGalley and Midnight Ink.
Kathleen began writing stories when she was just 10 years old. At 15 she wrote her first novel and she sold her first novel to a publisher 20 years later! For years she wrote while working at other day jobs. She spent 12 years working at a huge historic site, which was a perfect spot for someone interested in historical fiction. She also developed and scripted instructional videos for public television. Now she writes full-time.
The Lacemaker's Secret starts with curator Chloe Ellefson who heads to Green Bay for a consulting job at Heritage Hill Historical Park, where an old Belgian-American farmhouse is being restored. She hopes it will be a relaxing escape, because she has just discovered a unsettling family secret and her boyfriend, Roelke McKenna has been acting strange lately. She could use a distraction and is looking forward to working on the historical restoration of the Belgian farmhouse and meeting up with her old friend Elise O'Rourke. Elise is a lace scholar, who is also going to Green Bay for her job. She is looking for a rare and valuable piece of lace made by one of the earliest Belgian settlers.
What was supposed to be a delightful assignment, soon turns into a mysterious scene. When driving up to the Belgian Acres B&B, Chloe sees a century-old summer bake oven and she decided to take a look. She discovers a body in the bake oven..
The person in the bake oven is murdered and research suggests that the crime has something to do with the valuable piece of lace Elise is looking for.
The Lacemaker's Secret is written from two perspectives. The first narrative is from Chloe. The second narrative is the story of Seraphine Morreau Lejeune, a Belgian lacemaker and early immigrant from mid-1800. Seraphine's narrative is a story about hope, love, sisterhood, grieve and perseverance.
I adored reading the historical timeline and I really liked the mystery part of the story too. I do think I would have enjoyed the book even more if I'd read the other books in the Chloe Ellefson series too. This is the ninth novel in the series and, although you can definitely read it as a stand-alone, I do think it would give the characters more depth if you read all the books.


Fashion isn't necessarily elusive. It may seem hard if you have a general lack of time. There are some basic things you should know. Keep reading for some great fashion tips.
You can easily dress up your jeans with a fancy top and a nice pair of heels. If you're wearing the latest trend, colored jeans, it's best to go for a more casual look.
Create your own special style. Too many individuals try to copy others; looking great often involves putting your own spin on things. Having a comfortable personality is key, and when you create your style, you'll be praised on it's originality.
There are countless attractive options in accessories for your hair. Some hair accessories you can use are headbands, ponytail holders and things like hair extensions. No matter the length of your hair, be sure to include some hair accessories in your wardrobe for every occasion. For instance, if you desire a sporty look, put on a sporty outfit with a ponytail holder that matches. So before you go out, make sure you're carrying a few accessories with you.
Perfection is almost impossible with a subject like fashion. First, there are no perfect people, and it is an unattainable goal. Also, when you seek perfection, you are trying to hard. Many of the greatest looks of all time come from people who are not afraid to emphasize their flaws.
Clean out your closet periodically. It might appear that more clothing means more options, but that is opposite of the real truth. A cramped, overflowing closet makes it more difficult to choose outfits. Make the time to clear out items that have seen little use or do not fit anymore. Having a few staple pieces in your wardrobe is much more useful than having outdated clothing hanging in your closet.
Fashion is typically viewed as being about clothing. What some fail to consider is how much a bad hair style can hinder an otherwise great ensemble. So if you want to keep things looking fashionable, pay attention to your hair, to the accessories you're wearing, to your shoes, etc. Don't have anything on you that's out of place.
You should wear white any time of year, Labor Day or otherwise! There are year round variations of white and every other color, and it is important to wear colors that flatter you. If you look best in white, by all means, wear it all year 'round. If someone says you're wrong, walk away with a bounce in your step and a smile on your face knowing you look fantastic.
It doesn't take a ton of time to concentrate on improving your fashion. You can quickly and easily bring forth your personal style. This article has shown you the way, now follow the path to high fashion!


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As we approach the end of Precipice Fund's third round of funding and get ready to announce our Round 4 grantees on December 13, we've been checking in with our current round of grantees to see how their projects are progressing. The update below comes from the team behind the "experimental 'filmed zine'" project My Rock 'n Roll Fantasy in late May 2016. Along with their update, they delivered a trailer for the film.
Jen likes heroin is a 5 minute animation of two teenaged girls' introduction to heroin addiction in Riot grrl era Portland. The animation is part of an experimental documentary hybrid centering around the death of Molly 16, Portland feminist and troublemaker. Molly took her life at the age of 19 after struggling with homelessness and abuse. The story is taken from Molly's zine, Rock n Roll Fantasy, also the name of the film. Jen was a homeless street worker, who offered "Free Drugs" to anyone outside of the all ages rock club one night. 16-year-old Amber and Molly took her up on it.
The animation takes place in front of the X-Ray Cafe and inside the Howling Frog, two long gone haunts in Old Town. Amber has been writing the script with a final deadline of May 31. Willow has drawn initial character and location sketches. Actress Samantha Turret has been contacted in New York and we are all excited to collaborate in motion in June!
To learn more about the project, visit their website or like their page on Facebook.


Victor Manuel Quiles Bernard, 83, of Bethlehem, died October 15 at his home.
Services on Friday, Oct. 21 in Corozal, Puerto Rico. Local arrangements by Nicos C. Elias Funeral Home, Allentown., Arrangements in Puerto Rico by Funeraria Hernandez, Corozal.


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Florida Senate - 2017                      CS for CS for SB 1210
By the Committees on Appropriations; and Education; and Senators
Lee, Mayfield, Steube, Hutson, Artiles, Bean, and Passidomo
576-04166-17                                          20171210c2
1                        A bill to be entitled
2         An act relating to instructional materials for K-12
3         public education; amending s. 1006.28, F.S.; revising
4         the term "adequate instructional materials"; defining
5         terms; requiring each district school board to adopt a
6         process allowing parents or residents of the county to
7         object to the use of specific instructional materials
8         based on specified criteria; requiring the process to
9         include a right to appeal a school district decision;
10         specifying the appeal process; deleting a provision
11         relating to the finality of the school board's
12         decision under certain circumstances; requiring that
13         district school boards provide parents and residents
14         of the county access to certain materials under
15         certain circumstances; amending s. 1006.283, F.S.;
16         revising the requirements for school boards that adopt
17         rules for the implementation of the district's
18         instructional materials program; conforming provisions
19         to changes made by the act; amending s. 1006.31, F.S.;
20         revising the standards that an instructional materials
21         reviewer shall use; amending s. 1006.40, F.S.;
22         revising requirements for use of the instructional
23         materials allocation; revising the types of
24         instructional materials for which a district school
25         board is responsible; revising applicability; amending
26         ss. 1002.20 and 1006.42, F.S.; conforming cross
27         references; providing an effective date.
29  Be It Enacted by the Legislature of the State of Florida:
31         Section 1. Present subsections (1), (2), and (3) of section
32  1006.28, Florida Statutes, are redesignated as subsections (2),
33  (3), and (4), respectively, a new subsection (1) is added to
34  that section, and present subsection (1) and paragraph (a) of
35  present subsection (2), are amended, to read:
36         1006.28 Duties of district school board, district school
37  superintendent; and school principal regarding K-12
38  instructional materials.—
39         (1) DEFINITIONS.—As used in this section, the term:
40         (a) "Adequate instructional materials" means instructional
41  materials that meet the requirements of this section and have a
42  sufficient number of student or site licenses or sets of
43  materials that are available in bound, unbound, kit, or package
44  form and may consist of hardbacked or softbacked textbooks,
45  electronic content, consumables, learning laboratories,
46  manipulatives, electronic media, and computer courseware or
47  software to serve as the basis for instruction for each student
48  in the core subject areas of mathematics, language arts, social
49  studies, science, reading, and literature.
50         (b) "Instructional materials" has the same meaning as in s.
51  1006.29(2).
52         (c) "Legal resident" or "resident" means a person who has
53  maintained his or her residence in this state for the preceding
54  year, has purchased a home that is occupied by him or her as his
55  or her residence, or has established a domicile in this state
56  pursuant to s. 222.17.
57         (2)(1) DISTRICT SCHOOL BOARD.—The district school board has
58  the constitutional duty and responsibility to select and provide
59  adequate instructional materials for all students in accordance
60  with the requirements of this part. The term "adequate
61  instructional materials" means a sufficient number of student or
62  site licenses or sets of materials that are available in bound,
63  unbound, kit, or package form and may consist of hardbacked or
64  softbacked textbooks, electronic content, consumables, learning
65  laboratories, manipulatives, electronic media, and computer
66  courseware or software that serve as the basis for instruction
67  for each student in the core subject areas of mathematics,
68  language arts, social studies, science, reading, and literature.
69  The district school board also has the following specific duties
70  and responsibilities:
71         (a) Courses of study; adoption.—Adopt courses of study,
72  including instructional materials, for use in the schools of the
73  district.
74         1. Each district school board is responsible for the
75  content of all instructional materials used in a classroom,
76  whether adopted and purchased from the state-adopted
77  instructional materials list or, adopted and purchased through a
78  district instructional materials program under s. 1006.283, or
79  otherwise purchased or made available in the classroom.
80         2. Each district school board shall must adopt a policy
81  regarding an objection by a parent or a resident of the county a
82  parent's objection to the his or her child's use of a specific
83  instructional material, which clearly describes a process to
84  handle all objections and provides for resolution. The process
85  must provide the parent or resident the opportunity to offer
86  evidence to the district school board that:
87         a. An instructional material does not meet the criteria of
88  s. 1006.31(2) if it was selected for use in a course or
89  otherwise made available to students in the school district but
90  was not subject to the public notice, review, comment, and
91  hearing procedures under s. 1006.283(2)(b)8., 9., and 11.
92         b. Any material used in a classroom, made available in a
93  school library, or included on a reading list contains content
94  that is pornographic or prohibited under s. 847.012, is not
95  suited to student needs and their ability to comprehend the
96  material presented, or is inappropriate for the grade level and
97  age group for which the material is used.
99  If the district school board finds that an instructional
100  material does not meet the criteria under sub-subparagraph a. or
101  that any other material contains prohibited content under sub
102  subparagraph b., the school district shall discontinue use of
103  the material for any grade level or age group for which such use
104  is inappropriate or unsuitable. The process must also include a
105  right to timely appeal any district decision to the district
106  school board.
107         3. Each district school board shall must establish a
108  process by which the parent of a public school student or a
109  resident of the county may contest the district school board's
110  adoption of a specific instructional material. The parent or
111  resident must file a petition, on a form provided by the school
112  board, within 30 calendar days after the adoption of the
113  material by the school board. The school board must make the
114  form available to the public and publish the form on the school
115  district's website. The form must be signed by the parent or
116  resident, include the required contact information, and state
117  the objection to the instructional material, based on the
118  criteria of s. 1006.31(2). Within 30 days after the 30-day
119  period has expired, the school board must, for all petitions
120  timely received, conduct at least one open public hearing on all
121  petitions timely received, providing at a minimum the procedural
122  safeguards of ss. 120.569 and 120.57 making appropriate
123  provision for appointment of unbiased and qualified hearing
124  officers. A hearing officer may not be an employee, agent, or
125  contractor of the school district and provide the petitioner
126  written notification of the date and time of the hearing at
127  least 7 days before the hearing. all instructional materials
128  contested must be made accessible online to the public at least
129  7 days before a public hearing.
131  The school board's decision after convening a hearing is final
132  and not subject to further petition or review.
133         (b) Instructional materials.—Provide for proper
134  requisitioning, distribution, accounting, storage, care, and use
135  of all instructional materials and furnish such other
136  instructional materials as may be needed. Instructional
137  materials used must be consistent with the district goals and
138  objectives and the course descriptions established in rule of
139  the State Board of Education, as well as with the applicable
140  Next Generation Sunshine State Standards provided for in s.
141  1003.41.
142         (c) Other instructional materials.—Provide such other
143  teaching accessories and aids as are needed for the school
144  district's educational program.
145         (d) School library media services; establishment and
146  maintenance.—Establish and maintain a program of school library
147  media services for all public schools in the district, including
148  school library media centers, or school library media centers
149  open to the public, and, in addition such traveling or
150  circulating libraries as may be needed for the proper operation
151  of the district school system. Within 30 days after receiving a
152  written request, a school district shall provide access to any
153  instructional material or book specified in the request which is
154  maintained in a district school system library and is available
155  for review.
156         (3)(2) DISTRICT SCHOOL SUPERINTENDENT.—
157         (a) The district school superintendent has the duty to
158  recommend such plans for improving, providing, distributing,
159  accounting for, and caring for instructional materials and other
160  instructional aids as will result in general improvement of the
161  district school system, as prescribed in this part, in
162  accordance with adopted district school board rules prescribing
163  the duties and responsibilities of the district school
164  superintendent regarding the requisition, purchase, receipt,
165  storage, distribution, use, conservation, records, and reports
166  of, and management practices and property accountability
167  concerning, instructional materials, and providing for an
168  evaluation of any instructional materials to be requisitioned
169  that have not been used previously in the district's schools.
170  The district school superintendent shall must keep adequate
171  records and accounts for all financial transactions for funds
172  collected pursuant to subsection (4) (3).
173         Section 2. Subsections (1), (2), and (4) of section
174  1006.283, Florida Statutes, are amended to read:
175         1006.283 District school board instructional materials
176  review process.—
177         (1) A district school board or consortium of school
178  districts may implement an instructional materials program that
179  includes the review, recommendation, adoption, and purchase of
180  instructional materials pursuant to the requirements of this
181  section. The district school superintendent shall certify to the
182  department by March 31 of each year that all instructional
183  materials for core courses used by the district are aligned with
184  applicable state standards. A list of the core instructional
185  materials that will be used or purchased for use by the school
186  district shall be included in the certification.
187         (2)(a) If a district school board chooses to implement its
188  own instructional materials program, the school board shall
189  adopt rules implementing the district's instructional materials
190  program which must include its processes, criteria, and
191  requirements for the following:
192         1. Selection of reviewers, at least one-third one or more
193  of whom must be parents with children in public schools who are
194  not and have not been employees of the district.
195         2. Review of instructional materials.
196         3. Selection of instructional materials, including a
197  thorough review of curriculum content.
198         4. Reviewer recommendations.
199         5. District school board adoption.
200         6. Purchase of instructional materials.
201         7. Use of an instructional materials review committee that
202  is subject to s. 286.011 and that is selected by and reports
203  directly to the district school board.
204         (b) District school board rules shall must also:
205         1. Identify, by subject area, a review cycle for
206  instructional materials.
207         2. Specify the qualifications for an instructional
208  materials reviewer and the process for selecting reviewers; list
209  a reviewer's duties and responsibilities, including compliance
210  with the requirements of s. 1006.31; and provide that all
211  instructional materials recommended by a reviewer be accompanied
212  by the reviewer's statement that the materials align with the
213  state standards pursuant to s. 1003.41 and the requirements of
214  s. 1006.31.
215         3. State the requirements for an affidavit to be made by
216  each district instructional materials reviewer which
217  substantially meet the requirements of s. 1006.30.
218         4. Comply with s. 1006.32, relating to prohibited acts.
219         5. Establish a process that certifies the accuracy of
221         6. Incorporate applicable requirements of s. 1006.31, which
222  relates to the duties of instructional materials reviewers.
223         7. Incorporate applicable requirements of s. 1006.38,
224  relating to the duties, responsibilities, and requirements of
225  publishers of instructional materials.
226         8. Establish the process by which instructional materials
227  are adopted by the district school board, which must include:
228         a. A process to allow student editions of recommended
229  instructional materials to be accessed and viewed online by the
230  public at least 20 calendar days before the school board hearing
231  and public meeting as specified in this subparagraph. This
232  process must include reasonable safeguards against the
233  unauthorized use, reproduction, and distribution of
234  instructional materials considered for adoption.
235         b. An open, noticed school board hearing to receive public
236  comment on the recommended instructional materials.
237         c. An open, noticed public meeting to approve an annual
238  instructional materials plan to identify any instructional
239  materials that will be purchased through the district school
240  board instructional materials review process pursuant to this
241  section. The district school board shall hold this public
242  meeting must be held on a different date than the school board
243  hearing.
244         d. Notice requirements for the school board hearing and the
245  public meeting that must specifically state which instructional
246  materials are being reviewed and the manner in which the
247  instructional materials can be accessed for public review.
248         9. Establish the process by which the district school board
249  shall receive public comment on, and review, the recommended
251         10. Establish the process by which instructional materials
252  are will be purchased, including advertising, bidding, and
253  purchasing requirements.
254         11. Establish the process by which the school district
255  notifies will notify parents and residents of the county of
256  their ability to access their children's instructional materials
257  through the district's local instructional improvement system
258  and by which the school district will encourage parents and
259  residents of the county to access the system. This notification
260  must be displayed prominently on the school district's website
261  and provided annually in written format to all parents of
262  enrolled students.
263         (4) Instructional materials that have been reviewed by the
264  district instructional materials reviewers and approved shall be
265  must have been determined to align with all applicable state
266  standards pursuant to s. 1003.41 and the requirements in s.
267  1006.31. The district school superintendent shall annually
268  certify to the department that all instructional materials for
269  core courses used by the district are aligned with all
270  applicable state standards and have been reviewed, selected, and
271  adopted by the district school board in accordance with the
272  school board hearing and public meeting requirements of this
273  section.
274         Section 3. Subsection (2) of section 1006.31, Florida
275  Statutes, is amended to read:
276         1006.31 Duties of the Department of Education and school
277  district instructional materials reviewer.—The duties of the
278  instructional materials reviewer are:
279         (2) EVALUATION OF INSTRUCTIONAL MATERIALS.—To use the
280  selection criteria listed in s. 1006.34(2)(b) for instructional
281  materials reviewers under a state approval process or for
282  instructional materials reviewers under a district approval
283  process under s. 1006.283 and recommend for adoption only those
284  instructional materials that are aligned with the Next
285  Generation Sunshine State Standards provided for in s. 1003.41.
286  Instructional materials recommended by each reviewer shall be,
287  to the satisfaction of each reviewer, accurate, objective,
288  balanced, noninflammatory, current, and suited to student needs
289  and their ability to comprehend the material presented.
290  Reviewers shall consider for recommendation materials developed
291  for academically talented students, such as students enrolled in
292  advanced placement courses. When recommending instructional
293  materials, each reviewer shall:
294         (a) Include only instructional materials that accurately
295  portray the ethnic, socioeconomic, cultural, religious,
296  physical, and racial diversity of our society, including men and
297  women in professional, career, and executive roles, and the role
298  and contributions of the entrepreneur and labor in the total
299  development of this state and the United States.
300         (b) Include only materials that accurately portray,
301  whenever appropriate, humankind's place in ecological systems,
302  including the necessity for the protection of our environment
303  and conservation of our natural resources and the effects on the
304  human system of the use of tobacco, alcohol, controlled
305  substances, and other dangerous substances.
306         (c) Include materials that encourage thrift, fire
307  prevention, and humane treatment of people and animals.
308         (d) Require, when appropriate to the comprehension of
309  students, that materials for social science, history, or civics
310  classes contain the Declaration of Independence and the
311  Constitution of the United States. A reviewer may not recommend
312  any instructional materials that contain any matter reflecting
313  unfairly upon persons because of their race, color, creed,
314  national origin, ancestry, gender, religion, disability,
315  socioeconomic status, or occupation.
317  1006.40, Florida Statutes, are amended to read:
318         1006.40 Use of instructional materials allocation;
319  instructional materials, library books, and reference books;
320  repair of books.—
321         (3)(a) Except for a school district or a consortium of
322  school districts that implements an instructional materials
323  program pursuant to s. 1006.283 Beginning with the 2015-2016
324  fiscal year, each district school board shall use at least 50
325  percent of the annual allocation for the purchase of digital or
326  electronic instructional materials that align with state
327  standards and that are included on the state-adopted list,
328  except as otherwise authorized in paragraphs (b) and (c).
329         (b) Up to 50 percent of the annual allocation may be used
330  for:
331         1. The purchase of instructional materials, including
332  library and reference books and nonprint materials;, not
333  included on the state-adopted list and for the repair and
334  renovation of textbooks and library books.
335         2. The purchase of materials that are not provided under
336  subparagraph 1. and that have intellectual content that assist
337  in the instruction of a subject or course. These materials may
338  be available in bound, unbound, kit, or package form and may
339  consist of hardbacked or softbacked textbooks, novels,
340  electronic content, consumables, learning laboratories,
341  manipulatives, electronic media, computer courseware or
342  software, and other commonly accepted instructional tools as
343  prescribed by district school board rule; and
344         3. The repair and renovation of textbooks and library books
345  and replacements for items that were part of previously
346  purchased instructional materials.
347         (c) District school boards may use 100 percent of that
348  portion of the annual allocation designated for the purchase of
349  instructional materials for kindergarten, and 75 percent of that
351  instructional materials for first grade, to purchase materials
352  not on the state-adopted list.
353         (5) Each district school board is responsible for the
354  content of all instructional materials used in a classroom,
355  whether purchased from the state-adopted instructional materials
356  list or through a district instructional materials program under
357  s. 1006.283 an adoption process or otherwise purchased or made
358  available in the classroom. Each district school board shall
359  adopt rules, and each district school superintendent shall
360  implement procedures, which that:
361         (a) Maximize student use of the district-approved
362  instructional materials, whether purchased from the state
363  adopted instructional materials list or purchased through a
364  district instructional materials program under s. 1006.283.
365         (b) If the instructional materials are purchased from the
366  state-adopted instructional materials list, provide a process
367  for public review of, public comment on, and the adoption of
368  instructional materials which that satisfies the requirements of
369  s. 1006.283(2)(b)8., 9., and 11.; or, if purchased through a
370  district instructional materials program, implement a program
371  that fully complies with s. 1006.283.
372         (8) Subsections (3), (4), and (6) do not apply to a
373  district school board or a consortium of school districts which
374  that implements an instructional materials program pursuant to
375  s. 1006.283 except that, by the 2015-2016 fiscal year, each
376  district school board shall use at least 50 percent of the
377  annual instructional materials allocation for the purchase of
378  digital or electronic instructional materials that are aligned
379  align with state standards adopted by the State Board of
380  Education pursuant to s. 1003.41.
381         Section 5. Paragraphs (b) and (c) of subsection (19) of
382  section 1002.20, Florida Statutes, are amended to read:
383         1002.20 K-12 student and parent rights.—Parents of public
384  school students must receive accurate and timely information
385  regarding their child's academic progress and must be informed
386  of ways they can help their child to succeed in school. K-12
387  students and their parents are afforded numerous statutory
388  rights including, but not limited to, the following:
389         (19) INSTRUCTIONAL MATERIALS.—
390         (b) Curricular objectives.—The parent of each public school
391  student has the right to receive effective communication from
392  the school principal as to the manner in which instructional
393  materials are used to implement the school's curricular
394  objectives, in accordance with s. 1006.28(4)(a) the provisions
395  of s. 1006.28(3)(a).
396         (c) Sale of instructional materials.—Upon request of the
397  parent of a public school student, the school principal shall
398  must sell to the parent any instructional materials used in the
399  school, in accordance with s. 1006.28(4)(c) the provisions of s.
400  1006.28(3)(c).
401         Section 6. Section 1006.42, Florida Statutes, is amended to
402  read:
403         1006.42 Responsibility of students and parents for
404  instructional materials.—All instructional materials purchased
405  under the provisions of this part are the property of the
406  district school board. When distributed to the students, these
407  instructional materials are on loan to the students while they
408  are pursuing their courses of study and are to be returned at
409  the direction of the school principal or the teacher in charge.
410  Each parent of a student to whom or for whom instructional
411  materials have been issued, is liable for any loss or
412  destruction of, or unnecessary damage to, the instructional
413  materials or for failure of the student to return the
414  instructional materials when directed by the school principal or
415  the teacher in charge, and shall pay for such loss, destruction,
416  or unnecessary damage as provided under s. 1006.28(4) s.
417  1006.28(3).
418         Section 7. This act shall take effect upon becoming a law.


FACS profiles for cell cycle synchronization of HCT116 cells pulsed with EdU. Asynchronous (AS) HCT116 cells were treated with CDK4/6i (Palbociclib, 2 μM) for 20 hours to arrest in G0/G1 (middle panel), followed by a wash in fresh media for 8 hours to release into S phase (right panel).
In vitro inhibition of mouse corticotroph tumor cells by R-roscovitine. (A) Treatment of ACTH-secreting AtT20 cells with R-roscovitine (1-2 × 10-5 μM) led to decreased number of viable cells at 24 and 48 h, as depicted by Wst-1 proliferation assay (mean ± SE; **P < 0.01). (B) Western blot of protein extracts derived from AtT20 cells treated with vehicle or R-roscovitine. (C) R-roscovitine treatment (10 μM) for 48 h induced senescence as indicated by increased β-gal expression. (D) ACTH concentration by radioimmunoassays of culture medium from AtT20 cells treated with vehicle or R-roscovitine (mean ±SE; **P < 0.01 and ***P < 0.001). (E) Western blot of protein extracts derived from AtT20 cells treated with R-roscovitine. Vehicle is 0.2% DMSO.
SNS-032 (BMS-387032) has firstly been described as a selective inhibitor of CDK2 with IC50 of 48 nM in cell-free assays and is 10- and 20-fold selective over CDK1/CDK4. It is also found to be sensitive to CDK7/9 with IC50 of 62 nM/4 nM, with little effect on CDK6. Phase 1.
For MTT assays, cells (2,000 ~ 5,000 cells/well) were subcultured into 96-well plates according to their growth properties. Cell proliferation was assayed at 72 hr after treatment of SNS-032 by adding 20 μl of 5 mg/ml 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution per 100 μl of growth medium. After incubating for 3-4 h at 37°C, the media were removed and 150 µl/well of MTT solvent (either absolute DMSO or isopropanol containing 4 μM HCl and 0.1% Nonidet-40) was added to dissolve the formazan. The absorbance of each well was measured by ELx808 (BioTek, Winooski, VT) or Wallac Victor2 (Perkin-Elmer Life Sciences, Boston, MA) Microplate Reader. Viable cells are presented as percent of control, vehicle-treated cells.
(a) Western blot for RALB-GTP, total RALB and GAPDH proteins in KG1 AML cells treated for 8 h with DMSO or dinaciclib (representative of three independent experiments).
Flavopiridol (Alvocidib) competes with ATP to inhibit CDKs including CDK1, CDK2, CDK4, CDK6, and CDK9 with IC50 values in the 20-100 nM range. It is more selective for CDK1, 2, 4, 6, 9 versus CDK7. Flavopiridol is initially found to inhibit EGFR and PKA. Phase 1/2.
(A) After mitotic arrest and treatment with the proteasome inhibitor MG132, HeLa cells were treated with the indicated kinase inhibitors The cells were harvested, and the lysates were separated by SDS-PAGE and immunoblotted for phosphorylated RV[S/T]F (p-RV[S/T]F, top). The blot was quantified using ImageJ software and normalized to the mitotic sample (bottom). MLN8054, Aurora A and Aurora B inhibitor; ZM447439 and hesperadin, Aurora B inhibitors; BI2536, PLK1 inhibitor; flavopiridol, CDK1 inhibitor. n = 3. **P < 0.01, paired Student's t test.
AZD4573 is a potent inhibitor of CDK9 (IC50 of <0.004 μM) with fast-off binding kinetics (t1/2 = 16 min) and high selectivity versus other kinases, including other CDK family kinases.
Atuveciclib (BAY-1143572) is potent and highly selective PTEFb/CDK9 inhibitor with IC50 values of 13 nM for CDK9/CycT and the ratio of IC50 values for CDK2/CDK9 is about 100. Outside the CDK family, It inhibits GSK3 kinase with IC50 values of 45 nM and 87 nM for GSK3α and GSK3β respectively.
Abemaciclib is a cell cycle inhibitor selective for CDK4/6 with IC50 of 2 nM and 10 nM in cell-free assays, respectively.
NU2058 is an inhibitor of CDK2 with IC50 value of 17 μM in an isolated enzyme assay. It also potentiates melphalan (DMF 2.3), and monohydroxymelphalan (1.7), but not temozolomide or ionising radiation.
AT7519 is a multi-CDK inhibitor for CDK1, 2, 4, 6 and 9 with IC50 of 10-210 nM. It is less potent to CDK3 and little active to CDK7. Phase 2.
Lysates of MDM treated with five-fold dilutions of the indicated compounds (starting concentration: AT7519, 0.5 mmol/l, roscovitine 4 mmol/l) were subjected to SDS-PAGE, transferred, and immunoblotted with antiphopho-SAMHD1, anti-SAMHD1 and anti-Hsp90 antibodies. MDM, monocyte-derived macrophage; SAMHD1, sterile a motif and HD domain-containing protein-1; SD, standard deviation.
JNJ-7706621 treatment of tamoxifen-resistant cell lines leads to arrest in the G2 cell cycle phase. M phase cells from the upper right quadrants were quantified relative to total G2/M phase cells. Statistical significant differences from vehicle-treated cells are denoted by asterisks; *P<0.05, **P<0.01.
AZD5438 is a potent inhibitor of CDK1/2/9 with IC50 of 16 nM/6 nM/20 nM in cell-free assays. It is less potent to CDK5/6 and also inhibits GSK3β. Phase 1.
(a) HEK-293 cells expressing V5-EXO1 were synchronized in different phases of the cell cycle. EXO1 was immunoprecipitated (IP) with anti-V5 antibody and Western blotted with anti-phospho-Ser (p-SP) or anti-phospho-Thr (p-TP) CDK substrate antibodies, as indicated. "+AZD" indicates cells pre-treated with the CDK inhibitor AZD5438 prior to analysis. (b) HEK-293 cells expressing V5-EXO1, synchronized in G2, were pre-treated with CDK inhibitors, AZD5438 or Roscovitine, prior to analysis of EXO1 phosphorylation by IP-Western.
PHA-793887 is a novel and potent inhibitor of CDK2, CDK5 and CDK7 with IC50 of 8 nM, 5 nM and 10 nM. It is greater than 6-fold more selective for CDK2, 5, and 7 than CDK1, 4, and 9. Phase 1.
J558 cells were treated with PHA793887 (0.02–5 umol/L) for 6 hours, after which Western blot analysis was performed to monitor XBP-1s expression. Tub, tubulin.
BS-181 HCl is a highly selective CDK7 inhibitor with IC50 of 21 nM. It is more than 40-fold selective for CDK7 than CDK1, 2, 4, 5, 6, or 9.
Relative sensitivity of UKF-NB-3 and UKF-NB-3rSNS-032300nM to the CDK2, 7, and 9 inhibitor seliciclib, the CDK7 inhibitor LDC000067, the CDK9 inhibitor BS-181, or the CDK 1,2,4,6,7, and 9 inhibitor alvocidib.
SJSA cells were treated with the alternate CDK4/6 inhibitors Ribociclib (LEE011) and Abemaciclib (LY2835219) for 30 h, alone or in combination with Nutlin at 20 µM for 6 h. Immunoblot analysis was performed as in (a). Like Palbociclib (PD0332991), the combination of Nutlin with alternate CDK4/6 inhibitors diminished p53 target gene expression when compared to Nutlin treatment alone.
BMS-265246 is a potent and selective CDK1/2 inhibitor with IC50 of 6 nM/9 nM in a cell-free assay. It is 25-fold more selective for CDK1/2 than CDK4.
PHA-767491 is a potent ATP-competitive dual Cdc7/CDK9 inhibitor with IC50 of 10 nM and 34 nM in cell-free assays, respectively.It displays ~20-fold selectivity against CDK1/2 and GSK3-β, 50-fold selectivity against MK2 and CDK5, 100-fold selectivity against PLK1 and CHK2.
Viability at 250 nM and CI vs. Fa for U2932 following 24 hours exposure to ABT-199, additional drugs with activity against CDK9, or the combinations. Mean of quadruplicates ± SEM.
R547 is a potent ATP-competitive inhibitor of CDK1/2/4 with Ki of 2 nM/3 nM/1 nM. It is less potent to CDK7 and GSK3α/β, while inactive to other kinases. Phase 1.
IB analysis of whole cell lysates derived from mouse CT26 or 4T1 tumor cell lines treated with or without the CDK4/6 inhibitor, ribociclib.
NU6027 is a potent ATR/CDK inhibitor, inhibits CDK1/2, ATR and DNA-PK with Ki of 2.5 μM/1.3 μM, 0.4 μM and 2.2 μM, enter cells more readily than the 6-aminopurine-based inhibitors.
P276-00 is a novel CDK1, CDK4 and CDK9 inhibitor with IC50 of 79 nM, 63 nM and 20 nM, respectively. Phase 2/3.
Predicted anticancer drugs inhibit the growth of neuroendocrine tumor cells. Multi-tyrosine kinase inhibitors sorafenib, sunitinib, regorafenib and cabozantinib effectively inhibit GOT1 cell growth with IC50 values in the micromolar range. Inhibitors of AKT (MK-2206), HDAC (vorinostat), HSP90 (alvespimycin) and CDK4/9 (P276-00) also inhibited GOT1 cell growth at micromolar concentrations, whereas inhibition of PARP1 (veliparib) had no effect. GOT1 cells were treated with anticancer drugs at various concentrations for 4 days. Cell viability was estimated using AlamarBlue®. Data points are the mean values of three individual experiments carried out in triplicate (n=9). Fitting of curves was done in GraphPad Prism software v6.04 using log (inhibitor) vs response nonlinear fit with variable slope. IC50 was interpolated at Y=50 and bars denote ±s.d.
THZ1 is a covalent CDK7 inhibitor which has the unprecedented ability to target a remote cysteine residue located outside of the canonical kinase domain, providing an unanticipated means of achieving selectivity for CDK7.
One and three days after differentiation induction, protein level of Myh3 and Myogenin were analyzed by western blot.
TG003 is a potent and ATP-competitive Cdc2-like kinase (Clk) inhibitor with IC50 of 20 nM, 200 nM, and 15 nM for Clk1, Clk2, and Clk4, respectively. No inhibitory effect on Clk3, SRPK1, SRPK2, or PKC.
HeLa cells were untreated or treated with either colcemid (10 μg/ml for 16 h), RO-3306 (9 μM for 16 h), or both colcemid and RO-3306, as indicated. Cells were then lysed, and proteins were detected by Western analysis.
LDC4297 is a novel CDK7 inhibitor (IC50=0.13±0.06 nM for CDK7 versus IC50s between 10 nM and 10,000 nM for all other analyzed CDKs).
Senexin A is a potent and selective inhibitor of CDK8 and its nearest relative, CDK19 with Kd values of 0.83 μM and 0.31 μM for CDK8 and CDK19 ATP site binding, respectively.
MSC2530818, a CDK8 inhibitor with the IC50 of 2.6 nM, displays excellent kinase selectivity, biochemical and cellular potency, microsomal stability, and is orally bioavailable.
Wogonin, a natural and biologically-active flavonoid found in plants, is an inhibitor of CDK9 and does not inhibit CDK2, CDK4 and CDK6 at doses that inhibit CDK9 activity; Also inhibits N-acetyltransferase.
Effect of Wogonin on the translocation and transcriptional activity of NFATc1. U2OS cells stably transfected with EGFP-NFATc1, RANKL (100 ng/ml) and different concentrations of Wogonin (5.0, 1.0, 0.1 and 0.0 mM) were added and incubated for 24 h. The IN Cell™ Analyzer 1000 was used to observe the translocation of NFATc1. (A) DMSO was used as a control. RANKL (100 ng/ml) significantly stimulated the translocation of NFATc1; Wogonin (5 mM) significantly decreased the translocation of NFATc1 induced by RANKL (magnification, x200). (B) Statistics showed that Wogonin could decrease the translocation of NFATc1 into the nucleus induced by RANKL in a concentration-dependent manner. RAW264.7 cells were transfected with pGL3-promoter-NFATc1-RE, and then RANKL (100 ng/ml) and different concentrations of Wogonin (5.0, 1.0, 0.1 and 0.0 mM) were added and incubated for 24 h. (C) RANKL significantly increased the transcriptional activity of NFATc1 and Wogonin decreased the transcriptional activity of NFATc1 induced by RANKL in a concentration-dependent manner. n=3; *P<0.05 and ***P<0.001 compared with the RANKL group. NAFTc1, nuclear factor of activated T cells c1; EGFP, enhanced green fluorescent protein; DMSO, dimethylsulfoxide, RANKL, receptor activator of nuclear factor κB ligand; RE, response element.
(A)HeLa cells were treated with Nocodazole for 8 hours and then fixed. Before the cells were stained with phospho-specific antibody against S119 of Ajuba, they were pre-incubated with PBS (no peptide control), or non-phosphorylated (control) peptide, or the phosphorylated peptide used for immunizing rabbits. CDK1 inhibitors Purvalanol A (10 μM) together with MG132 (25 μM) were added 2 h before the cells were fixed (bottom two rows).(B)Experiments were done similarly as in (A) with phospho-specific antibody against Ser175 of Ajuba.
LY2857785 is a type I reversible and competitive ATP kinase inhibitor against CDK9(IC50=0.011 μM) and also inhibits other transcription kinases CDK8(IC50=0.016 μM) and CDK7 (IC50=0.246 μM).
K03861 is a type II CDK2 inhibitor with Kd of 50 nM, 18.6 nM, 15.4 nM, and 9.7 nM for CDK2(WT), CDK2(C118L), CDK2(A144C), and CDK2(C118L/A144C), respectlvely.
Kenpaullone is an ATP-competitive inhibitor of cyclin-dependent kinases (CDKs). It also inhibit glycogen synthase kinase 3β (GSK3β) with IC50 of 0.23 µM.
Effect of Kenpaullone on GSK-3β and CDKs. (A and B) Naïve CD4+ T cells were stimulated with anti-CD3/CD28 (TCR), TGF-β (5 ng/ml) and IL-6 (20 ng/ml) in the presence of the indicated Kenpaullone (Kenp) concentrations for 30 min (A) or 24 h (A and B).
Skp2 inhibitor C1 (SKPin C1) is a specific and selective small-molecule inhibitor of Skp2-mediated p27 degradation by reducing p27 binding through key compound-receptor contacts.
ON123300 is a potent and multi-targeted kinase inhibitor with IC50 of 3.9 nM, 5 nM, 26 nM, 26 nM, 9.2 nM and 11nM for CDK4, Ark5, PDGFRβ, FGFR1, RET, and Fyn, respectively.
LDC000067 is a highly selective CDK9 inhibitor with IC50 of 44 nM, 55/125/210/ >227/ >227-fold selectivity over CDK2/1/4/6/7.
Inhibition of CDK9 leads to a decrease in RNApol II activity and repetitive element expression. HEK293T cells were treated with DMSO control and the indicated amounts of LDC000067, a CDK9 inhibitor, for a total of 3 h. (A) Immunoblots showing the levels of phosphorylated RNApol II largest subunit (pRPB1), total RPB1 and GAPDH, in response to different doses of CDK9 inhibitor. (B) Quantification of immunoblots from 2 independent experiments in which each condition was performed in duplicates, and in which pRPB1 and total RPB1 protein levels were normalized to GAPDH or as pRPB1/Total RPB1 ratio. (C, D) qRT-PCR analyses show a significant response to CDK9 inhibition of repetitive elements belonging to the LTR (C) and SINE (D) classes. Statistical analyses were performed to compare all groups using a One-way ANOVA followed by Bonforreoni's multiple comparison test, *P < 0.05, **P < 0.01, #P < 0.0001.
AT7519 HCl is a multi-CDK inhibitor for CDK1, 2, 4, 6 and 9 with IC50 of 10-210 nM in cell-free assays. It is less potent to CDK3 and little active to CDK7. Phase 2.
(c) Effect of AT7519 treatment on RNA pol II occupancy at the PRCC gene. MM1.S cells were treated with either DMSO vehicle (blue) or 2 μM AT7519 (brown) for 6 h, followed by RNA pol II ChIP-seq analysis. Twenty-fold magnifications of the rpm/bp scale of these gene tracks are shown in the right panel to show the difference in reads for elongating RNA pol II. TR, RNA pol II traveling ratio. (d) Genome-wide binding average RNA pol II (ChIP-seq) on active promoters and gene bodies following treatment of MM1.S cells with DMSO vehicle (blue) or 2 μM of AT7519 (brown) for 6 h. Magnification of the rpm/bp scale at gene bodies is shown in the inset. The inset includes RNA polymerase II traveling ratio distributions (TR, mean) derived from MM1.S cells treated with DMSO (blue) or 2 μM AT7519 (red). (e) Chemical structures of the pan-CDK inhibitor AT7519 and its biotinylated counterpart bio-AT7519. (f) In vitro kinase assays with recombinant cyclin T-CDK9 complex in the presence of increasing concentrations of AT7519 or bio-AT7519. The derived IC50 values for each compound are shown. (g) Effect of AT7519 and bio-AT7519 on MM1.S cell proliferation. Cells were treated with varying concentrations of drug for 72 h as indicated. The derived EC50 values for each compound are shown.
XL413 (BMS-863233) is a potent and selective cell division cycle 7 homolog (CDC7) kinase inhibitor with IC50 of 3.4 nM, showing 63-, 12- and 35-fold selectivity over CK2, Pim-1 and pMCM2, respectively. Phase 1/2.
The protein levels of p-MCM2 and p-ERK in NOK, HN6 and Cal27 cells treated with 0, 10 and 20 μMXL413 were detected by western blotting.


Joy Unspeakable was among some of the first titles published by Covenant People's Books and has the distinction of being the only paperback published as a second edition. This special version features three expository sermons covering the entire books of 1st Peter, the Epistle of James and fragments of 2nd Peter II. This reissue even showcases some early concept art and computer graphics that ultimately lead up to this definitive and final printing.


Sen. Greg Albritton, R-Range, filed a lottery bill this week that would establish a state lottery and limit it to paper-based games — a move that has drawn opposition in the past from senators who say it could give a major gambling entity in Alabama a unique advantage over others.
"It's a simple lottery," Albritton said on Wednesday afternoon. "It gives people the right to vote as to whether to have a lottery or not. Straight paper lottery. That's it."
The bill will be in a Senate committee Thursday morning, along with other proposals filed by Sen. Jim McClendon, R-Springville, who has filed a bill that would give the state's four dog tracks access to the same gaming machines that the federally recognized Poarch Band of Creek Indians, who operate casinos in Atmore, Montgomery, and Wetumpka could obtain if a lottery came to Alabama.
"Once we get my bill through, owners and operators will get the confidence to open tomorrow and next week and next year, there's going to be job opportunities for Alabamians," he said.
"The reality is a lottery is a Class III game," Singleton said. "If we allow Class III gaming they will go full casino, as opposed to bingo," Singleton said.
Robert McGhee, the director of government relations for the Poarch Band of Creek Indians, said he hadn't looked at all Albritton's bill in detail, but said it would give "the people of Alabama what they want," an opportunity to vote on a lottery.
"The point is you still have to have a negotiated compact with the state," he said. "We do not operate outside of a compact."
Albritton's district includes Atmore, where the Poarch Band operate Wind Creek Casino.
"I speak to my constituents, the Poarch Band included, about this particular bill," he said, adding that "I haven't gotten a yes or a no."
Senate President Pro Tem Del Marsh, R-Anniston, said there will be a public hearing Thursday morning but no vote. McClendon said he expects a vote on both bills on Wednesday. Marsh had expressed doubts about McClendon's legislation, and seemed supportive of McClendon's legislation.
"I take it to be the most straight, simple lottery bill there is," Marsh said. "If that's the case, I think it's the one with the best case to get out of the legislature."
21 comments. Last comment 10 days ago by Coin Toss.
Maybe attempt #3 will come up. I hate the mentality of do as I say, not as I do...assuming some of these bozos cross state lines to play lotteries. Paper lottery sounds like PB/MM to me.
It must drive them bonkers with the several Indian Casinos there. Especially the one right on I-65. They can't regulate them on sovereign land.
I feel like this article is posted on LP and reposted every other month. They either get the lottery or THEY DON'T. Does it have to go through this many rounds of legislation?
The Alabama legislators can't make up their mind on what type of lottery they want. With all the successful states lotteries they could simply point at the best and say "that's what we want".
Some of those Southern States love to play hardball when it comes to the lottery. If it's not some pastors having a fit over gambling in their backyard, it's politicians not being able to make up their minds.Lots of panic down there, more like dazed and confused.
It's not just Southern states, consider Utah for instance.
Also it's interesting that in the parking lots of casinos on the Nevada -Utah border there are plenty of vehicles with Utah license plates.
Coin Toss, when l posted " Southern States " l meant those particular States that have or are thinking of having a State lottery. Utah & Nevada do not nor are contemplating having one, at this time. Mississippi & Alabama got strong resistance from the religious leadership community, as you well know. I don't think l was being unfair in my assessment.
For a very long time it was believed Tennessee would never get a lottery but we know what happened there.
Those who run any kind of gambling believe a player will always find 'action'..........as an example when Players (since bought out by Harrah's) came to Southern Illinois a lot os the staff were from Nevada........casino people.......and they found themselves patronizing American Legion bingo.
On bad weather days a lot of construction workers hit the casino there, and on snow days, school teachers....."well the buses can't get the kids to school but I can make it to the casino".
AS a side note, when casinos expanded and started out with river boats located in small towns, a lot of the resistance from religious leaders in the community was coming from Catholic churches that were worried about their bingo games having competition.
So you do agree with me CT - those States politicians & religious leaders you mentioned, joined the happy band of being dazed and confused. There is no daylight between these two groups.Whether the one is talking about bingo competition or gambling is a tax on the poor, its all vanilla. Glad we have come to an accord.
You're into one of those debates comparing apples to onions again!
It should be obvious to anybody that religion is one of the underlying reasons Alabama is having difficulties creating a state run lottery. Never read where the legislators (for or against?) believed construction workers and school teachers in Alabama will flock to stores to buy lottery tickets on inclement days. Have no idea how casino gambling is even related to you offering your opinion why Alabama is having problems creating a lottery.
And speaking of Utah and unrelated discussions, did you see where they finally joined the 21st century by repealing their unconstitutional 1973 law.
l have no problem with religion existing, what l have a problem with is religious leaders telling us what we should & should not do. Encroaching on people's desires or how they should spend their money etc etc. Prostitution existed in the time of the Christ, yet there no scripture of any of the disciples or Christ himself attempting to stamp it out. They were more interested in spreading the word, religious leaders should stick to what they think they know, which should be minding their own business... just saying.
Who do those religious leaders think they are???? How dare they think prostitution is wrong! Upholding family values and respecting women and telling people there are other options to selling your body.
Lol The only people who yell about prostitution not being a crime, are serial killers, pimps, and clients. But I guess publishing the names of the "Johns" can make those clients unhappy.
And Jesus did tell the woman caught in adultery to go and sin no more. And most people with any sort of an understanding of any basic religious principles, understands giving money to the poor. How dare those churches suggest helping the less fortunate .
Well, They need to mind their own business!. Of course, Jesus never spoke of helping the poor , your neighbor, etc.
I must have missed where you said "prostitution isn't or shouldn't be a crime"; maybe Craft said it. But I forgot some people believe it's necessary to make it looks like you said that.
While religion is one of many hurdles in trying to create an Alabama Lottery, bet the biggest is how the law makers will spend the profits. Now trying to figure out how adultery applies to the lottery news.
Please let's not talk about family values- lots of the folks who claim family values had no problem pulling the lever for you *know who, to be their leader. I will not get drawn out into a protracted debate , but suffice to say, One of Christ ancestors was a prostitute, shocked? Do research. No one is saying prostitution is right, on the contrary, l am saying the main focus of the Christ was not to condemn.Lastly, despite what you may think or say, your knowledge of Christianity is no match for mine.
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Model Jasmine Sanders says she's no longer 'scared' to embrace her body: 'You're going to see my cellulite, you're going to see stretch marks'
Kerry Justich
August 2, 2021, 5:23 PM ·4 min read
Jasmine Sanders talks body insecurities and being unfiltered. (Photo: Getty Images; designed by Quinn Lemmers)
It Figures is Yahoo Life's body image series, delving into the journeys of influential and inspiring figures as they explore what body confidence, body neutrality and self-love means to them.
Jasmine Sanders rocked a teeny bikini on the cover of Sports Illustrated Swimsuit in 2020 as she became the fourth Black woman to snag the coveted spot. Despite her comfortability in front of the camera for the sexy shoot, however, the 30-year-old tells Yahoo Life that she used to feel her most insecure in a piece of swimwear.
"I would not get out of my jeans and never really felt comfortable being in a bikini next to some of my girlfriends, because I was just the girl with a flat chest," she says of her younger self. "And mind you, that took forever to get past."
Sanders explains that she grew up in the South, where it's "extremely hot," which seemingly made her unwillingness to show off different parts of her body even more apparent.
"I had insecurities like every other girl. I wanted to look cute in my outfits. I wanted to fit in at school. And I was dealing with people bullying me for being too skinny and too athletic," she explains. "My dad used to joke with me all the time and be like, 'Hey, why are you always in jeans? Get out of your jeans. It's a million degrees outside.' And I was scared to show my legs."
The model and influencer was born in Germany to a German mother and Black father before moving to Columbia, S.C. where she struggled to figure out who she was and where she fit in. She says that race also played a role in that process.
"I wanted to be friends with everybody. I'm a social butterfly, but I was dealing with different kids that were kind of bullying me about whether I could have white friends or Black friends or if I had too many," Sanders recalls. "So I was dealing with racial things in the South and also trying to figure out my body and figure out being a teenager."
Despite not having the utmost confidence in herself, Sanders was discovered by a local modeling agency when she was a teenager. By 13, she found herself working to change an industry that abided by exclusive and unrealistic beauty standards in order to maintain her own happiness.
"Being in the industry at a young age, you're dealing with a lot of negativity. I was one of the shorter girls, I wasn't the go-to runway model. But I just tried to make a name for myself and get people to understand that my body's beautiful as well, even if I wasn't the sample size at the time," she explains. "I can work out as much as I can and eat right and do all of those things, but it's not until I really focus on loving myself and loving spending time with myself — spending time with myself in front of the mirror in a different way, saying my affirmations — that I'm going to feel most confident."
While Sanders felt that she was a "tomboy" that didn't fit into the "cookie cutter" image of what she believed a model to be, she found inspiration from women around her who also felt they were outside of the mold but exuded a confidence that Sanders envied. With the moniker "Golden Barbie" and a following of 4.5 million people on Instagram alone, Sanders tries to pass that inspiration on to others. One of the best ways to do so, she says, is by being unfiltered.
"If you're going to see me on a runway in a bikini, you are going to see my cellulite, you're going to see stretch marks because my body does fluctuate like everyone else's. You might see me eating tacos and relaxing by the pool, and I don't want to constantly put out super photoshopped photos," she shares. "I know that in the industry that I've come from a lot of things for a long time have been super photoshopped, but at this point in my life, I just want to embrace everything that God has allowed my body to do and become and I don't want to hide it from anybody."
While Sanders now says with confidence "I absolutely love my body and have learned to embrace every bit of change and every curve," she acknowledges that experiencing that type of security still comes in waves. She also credits turning 30 for her current "no f***s given" state of mind.
Most importantly, she reflects on what she'd tell her younger self about letting go of the insecurities that once held her back. "I would tell her to go for it and actually put a bikini on and go and have fun and not to sit back and miss out on pool parties," Sanders says. "Just live your life, embrace everything that comes your way. And I swear, you'll have a lot more fun."
The former star of TLC's "19 Kids and Counting" has had a change in her beliefs since leaving the church.
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Inside cookbook author Grace Young's work to revitalize Chinatown businesses hit by pandemic: 'The most meaningful work I've ever done'
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The 56-year-old actress left little to the imagination on Wednesday night.


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By Laura van de Vorst Cause & Company, Food & Nutrition June 24, 2014
Kulinary Kindness | 3 Delicious, Nourishing & Social Brands
Delicious, Nourishing & Social
Being a curious and conscious health freak, I recently came across several kind-hearted brands that I would love to share with you. Brands that produce all-natural, gluten-free and healthy foods WITH a social mission. Whether they are co-creating and sourcing in rural communities; empowering women in developing countries; creating economic opportunities; tackling global malnutrition; or improving public health – these brands are breaking with business conventions and try to achieve a real societal change with tasty, wholesome, and beautifying products.
After a few months serving in the Peace Corps in Niger, Lisa Curtis experienced early signs of maltnutrition. Someone told her to take some Moringa leaves. This superplant requires little water and flourishes in harsh climates. It made her feel better.
Upon return, Lisa wanted to continue to support women in West Africa to grow more moringa and utilize it to improve the health of their families and communities. Together with a few friends she started to experiment and research to make an impact on global nutrition through Moringa. The idea for the Kuli Kuli bar was born.
"We source sustainably harvested Moringa from women's cooperatives in West Africa", Lisa explains in Kuli Kuli's Indiegogo Campaign film. Kuli Kuli supports West African women to grow more Moringa and creates economic opportunity.
Besides Moringa, Kuli Kuli bars contain simple all natural ingredients, such as dates, black cherry, almond, honey, and chocolate. This promising healthy gluten-free and raw snack is filled with fiber, protein, vitamins and antioxidants. The ambition and commitment of the founders is great. At the moment they only have the capacity to sell at local farmers' markets in the US. "The thing is, we can't keep up with the demand. It takes three of us six hours to make 200 bars — the same amount of bars that a factory could make in less than 2 minutes. So we need your help to produce our first manufacturing run of these bars and bring them to the world."
Hereunder you can find how the first 50,000$ is invested to scale the social and economic impact of the young start-up.
We often experience that moment – on-the-go or during working hours – in which one cannot find a single unprocessed or healthy snack around. And sometimes in the rush of tasks and deadlines, time doesn't allow you to prepare a responsible-and-good-for-the-body meal to take with wherever you're heading. A problem that for Daniel Lubetzky became a light bulb moment.
Lubetzky is founder of Peaceworks Inc., Peaceworks Foundation's OneVoice Movement and co-founder at Maiyet. A social entrepreneur whom TIME Magazine recognized in 2009 among "25 Responsibility Pioneers" and BusinessWeek named among "America's Most Promising Social Entrepreneurs." Unhappy with unhealthy snacking options, and concerned with the rising obesity and diabetes epidemic in America, he decided to launch KIND Snacks. "KIND to your body, your taste buds and the world."
Do the KIND Thing from KIND Healthy Snacks on Vimeo.
The products are very tasty in my opinion, and leave you with a no guilt. In the natural food industry (NPEE), KIND got awarded "Best Product of the Year" and is the fastest-growing healthy snack in the nutrition bar category. Since 2003, the product range has developed from fruit and nut bars, to nuts and spices, granolas and to KIND Plus bars with nutritional boosters. All consisting of wholesome gluten-free ingredients and superfoods. Ten years ago a fresh and now a still very welcome breeze in a food category often associated with high amounts of wheat, chemical additives and an overdose of sugar.
Besides your body and taste buds, KIND is good to the world too. With marketing campaigns such as KINDAHOLICS, Do The KIND Thing and #KindAwesome, unexpected acts of kindness are encouraged resulting thousands of stories of selfless efforts.
And let's not forget the "empowering & beautifying wonder brand" Aduna. Just as Kuli Kuli, also Aduna product range is based on African flora – The Baobab Tree. Baobab is a nutrient-dense, raw wholefood with one of the
highest antioxidant capacities of any fruit in the world. Sourcing from community-owned trees in 32 countries all over Africa, Andrew Hunt and Nick Salter bring this Feel Good Fruit to the international market.
The Baobab – or The Tree of Life – grows an extraordinary fruit with outstanding qualities. "Baobab fruit contains fourteen essential vitamins and minerals. It is almost fifty percent fibre and has one of the highest antioxidant capacities of any fruit in the world. Baobab is an excellent source of vitamin C, calcium, potassium, thiamin and a good source of vitamin B6. These nutrients gives it an amazing range of benefits, supporting with energy metabolism, the immune system and promote good skin health." For centuries the women of Africa have turned to the baobab tree as a source of natural wellbeing, benefiting skin, hair and general health.
Aduna has a positive impact in rural communities; creating demand for under-utilised but remarkable natural products from small-scale producers in Africa. On top of that Aduna invests part of the profit in the Aduna Foundation, investing in strategically relevant social projects, helping small-scale producers increase their quality of the production and processing and supporting them to overcome exclusion by EU strict laws for health and beauty products.
Unlike other fruits, baobab dries naturally on the branch; it is simply harvested, then the
seeds removed and sieved into a powder. It is a 100% natural product. Aduna has made this
pure, nutritious fruit pulp available as a loose powder or as convenient single-serve stick
packs; ideal for adding to smoothies or juices, or using as an alternative to sugar for
sweetening yogurt, muesli or cereal. Capsules are also available for on-the-go ease.
What healthy and conscious foods do you indulge in?
Photo Courtesy: Aduna & Kuli Kuli
AdunaAfricaAndrew HuntBaobabBarCo-CreationCommunitiesConsciousDaniel LubetzkyEconomicEmpowermentFairtradegluten freeGranolaHealthKINDkuli kuliLisa CurtisMissionMoringaNaturalNick SalterNutritionOpportunityPositive ImpactSocialSocial EntrepreneurshipSuperfoodSuperfruitWholesome
Laura van de Vorst is nutritionist, speaker, blogger and co-founder of healthcoachFX. Laura and her team help women (and men) become the best version of themselves with a personal Nourish-Love-Grow approach. She further inspires and connects likeminded health rebels with her community and online magazine The Green Tribe. Adventures, yoga and sparkling conversations fuel her positive life energy and put the biggest smile on her face.
Latest Posts By Laura van de Vorst
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Health Heroes


The first BHS Ireland camp of 2018 is at Castle Leslie Estate, Co Monaghan from 16 to 18 February 2018.
We are privileged to have as our celebrity coach Eric Smiley FBHS. Eric is a Fellow of the British Horse Society and has competed in the equestrian sport of eventing at the highest level. He thrives on keeping things simple but effective and loves to develop all levels of horse and rider.
This is a great opportunity to get 2018 started in the best way for you and your equine best friend! What could be better than indulgent accommodation in the Village Cottages for you, luxurious stabling for your horse and superb instruction with state of the art facilities?
The facilities include a large modern indoor school, compact out door and a 100 acre cross-country field with a wide variety of fences to suit most levels of horse and rider. Of course there is also access to accompanied rides through the 25km of pathways on the estate.
You will eat delicious food, laugh and chat and exchange stories and valuable information amongst long standing and new friends!
Luxurious accommodation in the Village Cottages.
5 lessons or one lesson may be replaced by an accompanied hack on Castle Leslie Estate, which has 25 km of paths.
Also on offer is access to the Health Spa at The Lodge, treatments can be booked directly.
Spaces are limited so book quickly. The cost is £550 per person or the equivalent in euro. (Please note that a minimum of 16 paid up places is required for a camp to take place).
Having spent nearly ten years in a Cavalry Regiment he decided, at the age of 28, to take up a professional career with horses. Whilst in the Army he trained with Ben Jones, who was Equitation Officer at Melton Mowbray. Ben Jones represented G.B. in the Tokyo Olympics and won team gold medal at the Mexico Olympics. Of Eric, Ben was to comment, "He has a great natural flair for equitation. When jumping he has that gift of rhythm and timing on any type of horse". He trained at the world famous Talland School of Equitation achieving his British Horse Society Instructor qualification before being asked to return to Ireland to start up an equestrian establishment specialising in bringing on and competing event horses.
Eric is also an FEI International Judge who regularly acts as a Ground Jury member and is Director of the International Eventing Forum (IEF). He was Team Coach to the Belgian eventing team for the London Olympics 2012 and the European Championships 2011.
Pre-booking is essential. You may pay by Paypal.


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Home » News » Amotekun: We will use ancestors' powers to defend South West ―Hunters
Amotekun: We will use ancestors' powers to defend South West ―Hunters
On January 15, 2020 12:47 amIn Newsby Rasheed Sobowale
Some Amotekun personnel during the launching ceremony
By Ola Ajayi – Ibadan
As the newly launched security outfit tagged Western Nigeria Security Network (tagged: Operation Amotekun) begins operations, one of its personnel, Soludero Hunters' Association has said the outfit doesn't rely on guns to function well but would use their ancestors' powers to ward off any aggression against Yoruba indigenes in the zone.
The National President of the hunters, Oba Nureni Ajijola Anabi who spoke with Vanguard said since the outfit was launched, they had resuscitated all "powers used by our ancestors to conquer their aggressors and that's what we will use in protecting our zone".
"We promise to be civil and law-abiding in our operations. We will never disappoint our governors who initiated the security outfit.
Also, the Ooni of Ife, Oba Eniitan Adeyeye Ogunwusi, the Alaafin of Oyo, Oba Lamidi Adeyemi, other prominent Yoruba kings, governors in the South-West zone, the Chairman of Road Transport Employers' Association of Nigeria, Prince Kehinde Adeyemo, Chairman of Oyo State Road Transport Management Agency, Mogaji Akin Fagbemi, Prof Adebanji Akintoye, Yoruba leader, should be rest assured that Amotekun will live up to expectation."
ALSO READ: Don't allow sycophants hijack Operation Amotekun, cleric warns state Govs
On the fears of Miyetti Allah that Amotekun was created against them, Ajijola who is also the Defence Minister of the RTEAN, Oyo State branch, noted that herders should not entertain any fear so far they "don't come to our lands and be terrorising our people.
We will not tolerate that any longer. We are not against any group provided they don't pose threats to our lives and property".
"Anyone who has the mind to kidnap, kill or rape our women should have a rethink. If not, we hunters in the South West will prove to them the stuff we are made of."
"It is only the people or group who want to kill, maim, kidnap and rape our women, destroy our livelihoods that should be afraid.
"We will match them strength with strength. We can't watch them commit atrocities on our lands. They can't take our lands from us.
READ ALSO: Buhari appoints DSS ex-DG, A.A. Gadzama as NISS Chairman
"We promise all Yorubas that they are secure anywhere in the zone because we have more than 500,000 hunters in each of the six states. We will not betray you
"As for the use of weapons, that may not be necessary. The powers of our ancestors are enough weapons. We don't need any approved weapon to operate. It was the powers that our forefathers used to win many battles in the past. They are still very much with us. We only need to revive them.
"All of us (personnel) in hunters' association will swear by god of iron. The meaning is that you don't betray, else god of iron will strike.
"Recently, thunders struck in some places and we all saw the effects. We can employ the services of these deities and they will answer us speedily
"We thank the governors for their courageous move to protect our people. In fact, our hunters are enough to check criminality. Our kings in the past engaged the services of hunters to protect their territories and their people were safe", he said.
South West's 'Amotekun' is illegal security outfit ―FG declares
Updated: Supreme Court sacks Ihedioha, declares Uzodinma winner