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Astaro Services AG Rheinweg 7, CH-8200 Schaffhausen. Supplementary data protection agreement. to the license agreement for license ID: between
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1 Astaro Services AG Rheinweg 7, CH-8200 Schaffhausen Supplementary data protection agreement to the license agreement for license ID: between represented by... Hereinafter referred to as the "Client" and Astaro Services AG, Rheinweg 7, CH-8200 Schaffhausen Hereinafter referred to as the "Contractor"
2 Table of Contents Preamble 3 1 Subject and duration of this agreement 3 2 Categories of affected individuals and types of saved data 4 3 Rights and responsibilities of the Client 4 4 General obligations of the Contractor 5 5 Technical and organizational measures 8 6 Amendment, blocking, and deletion of data 8 7 Deletion of data and return of data carriers 8 8 Damages 9 9 Security interests 9 10 General 9 Appendix I: 10 General technical and organizational measures 10 Page 2 of14
3 Preamble The Contractor was instructed to archive the Client's s. This agreement concluded in this respect shall hereinafter be referred to as the 'principal agreement'. Since the Contractor may be able to access the Client's personal data during the installation and adjustment of the storage system provided by the Contractor and during the subsequent maintenance and administration processes, commissioned data processing forms a part of this assignment. Since the Client is responsible for adhering to data protection regulations, the following must be agreed as a supplement to the principal agreement: 1 Subject and duration of this agreement 1) Insofar as it is relevant to this agreement, the principal agreement between the Contractor and Client relates to the archiving of s. Astaro Mail Archiving ('AMA') offers to archive s as a service provided by Astaro Services AG Rheinweg 7, CH-8200 Schaffhausen ('Astaro'). s which are to be archived are transmitted in encrypted form to the archiving service which can be accessed centrally via the internet. The s are then indexed, archived, and made available at any time for authorized user inquiries by the customer. 2) As a cloud-based solution, AMA offers excellent administration, scalability, availability, and security advantages. Astaro Group companies have many years' experience in internet security-related matters. This extensive expertise particularly enables Astaro to safeguard the data protection, access protection, and availability of AMA. 3) This document explains the internal processes and structure of AMA, as well as protective measures for comprehensive data and access protection. 4) The AMA infrastructure comprises a number of servers which process different types of task. 5) Astaro differentiates here between Production, Backup and Control Clouds whose operations are separated physically and geographically in separate hosting environments. This separation also serves to divide powers in order to maximize access and data protection. 6) This term of this commissioned data processing agreement is the same as that of the principal agreement, including any subsequent agreements, and shall expire on the same date as the principal agreement or the final subsequent agreement. This is without prejudice to the confidentiality obligations agreed in section 4 (2). Page 3 of14
4 2 Categories of affected individuals and types of saved data The areas of activity/services specified in section 1 result may result in access to personal data. However, this is only in encrypted form. 3 Rights and responsibilities of the Client 1) The Client alone is responsible for assessing the legitimacy of the data processed/collected/used and for safeguarding the rights of the individuals affected. The Client remains the owner of the data. 2) As far as access to personal data is concerned, the Client is authorized to issue the Contractor with instructions. The Client's data must be handled only according to the stipulations agreed (in both this agreement and the principal agreement) and the Client's instructions. The Client retains the right to issue instructions with regard to the methods, scope, and processes applied to the data processing and may substantiate this right in individual cases. Any changes to the data to be processed or to the procedures used must be agreed and recorded by both parties. The Contractor may only disclose information to third parties or affected individuals with the Client's prior written consent. The following individuals will act as contacts for the execution of this agreement on behalf of each party: On behalf of the Client:... [Name, contact details] The data protection officer named in section 4 (1) shall act as the central point of contact for the Contractor. Any changes must be immediately notified to the other contracting party. If, in the opinion of the Contractor, an instruction infringes data protection regulations, the Contractor shall immediately inform the Client of this. In this case, the Contractor is entitled not to carry out the particular instruction until it has been confirmed or amended by the employees of the Client.. Page 4 of14
5 3) Inspection rights The Client has the right, in agreement with the Contractor, to carry out the contractual inspections provided for by the law or assign these to other inspectors, to be named at a later date. Before the data is processed and for the duration of this agreement, the Client is also entitled to ensure that the technical and organizational measures agreed upon are being adhered to by the Contractor. To this end, the Contractor shall provide evidence to the Client of the implementation of these technical and organizational measures, as described in the Appendix. Unless it relates only to this specific assignment, this evidence may be provided in the form of a recent certificate, reports or extracts from reports issued by independent authorities (such as financial auditors, data protection auditors, data protection officers, IT security officers, or quality auditors) or appropriate certification from an IT security or data protection audit (in accordance with the basic protection regulations set by the Federal Office for Information Security). The Client has the right to carry out spot checks, for which prior notification should be given, unless this contravenes the purpose of the checks, to ensure that the Contractor's business operations comply with the terms of this agreement. On request, the Contractor shall provide the Client with the information it requires in order to effectively exercise its inspection rights and submit the relevant documents. 4 General obligations of the Contractor 1) The Contractor has appointed a data protection officer who will carry out his duties in accordance with statutory regulations. This is currently: Mr. André Meuser Tel. +49 (89) HYPERLINK The Contractor shall inform the Client in writing should there be a change to the person appointed as data security officer. Page 5 of14
6 2) Data confidentiality In the course of remote maintenance or on-site intervention, the Contractor undertakes only to access data to the extent required in order for it to carry out the services required and to the extent permitted by the following conditions. The technical and organizational measures taken by the Contractor to process and store the Client's data must be designed to protect the confidentiality of the data at all times. Should any stored data content be disclosed to the Contractor, the Contractor shall ensure that it remains confidential. In fulfilling the agreement for the Client, the Contractor shall adhere to this confidentiality and data secrecy requirement and shall treat as confidential all knowledge of business and trade secrets obtained within the scope of this contractual relationship. The Contractor is required to ensure that all its employees who might come into contact with the Client's data agree in writing to maintain data confidentiality and provide evidence of this to the Client. The Contractor shall ensure that all individuals it assigns to process data or fulfill this agreement comply with the legal provisions applicable to confidentiality requirements and data protection and that any information obtained is not disclosed to third parties or exploited in any other way and to monitor the confidentiality obligations established in this way. These obligations continue to apply after the end of the contractual relationship. 3) Subcontracting The Contractor is permitted to subcontract work to the extent described in order to complete the assignment within the scope of this agreement. Further subcontracting is only permitted with Client's prior written consent. The Subcontractor should be subject to the same data protection requirements imposed on the Contractor by the Client. The Contractor must also ensure that the Client can also exercise its inspection rights over the Subcontractor. Page 6 of14
7 4) Obligation to inform The Contractor acknowledges that there is a statutory obligation to report any loss, unlawful transfer, or disclosure of personal data, should this arise. Should the Contractor suspect or have specific evidence that the Client's personal data has been disclosed to unauthorized third parties, it shall inform the Client of this, regardless of the cause. This obligation shall also apply in the event of serious disruption to operating procedures, any suspected infraction of personal data protection regulations, or other irregularities in the handling of the Client's personal data. The Contractor and the Client must mutually agree appropriate measures to safeguard the data and minimize the potentially negative consequences for the Client or the individuals affected. 5) Economical use of data The Contractor shall restrict all access to the Client's data to the minimum level stipulated in the primary agreement. Should it be absolutely essential to access personal data, the Contractor must inform the Client before this data is accessed. The Client shall decide how to proceed in such an event. Should the Client grant access to certain personal databases, the agreed work may only be carried out on these databases. If personal data is accessed during the performance of the agreed maintenance work, it may not be changed. Any changes made in error must be reported to the client. Changes to system files required in the course of the work must be recorded by the Contractor. At the request of the Client, these changes must be reversed once the work has been completed. 6) Transfer of memory content Should the assignment comprise the copying of saved data, the Contractor should employ a method that prevents the content of the databases from being displayed as far as possible. If original data is extracted for error analysis during the maintenance work, this data must be deleted once the procedure is complete. Page 7 of14
8 5 Technical and organizational measures The Contractor is obliged to implement technical and organizational measures and adhere to them for the duration of this agreement. The Contractor shall document these measures. Should an inspection by the Client reveal the need to adjust these measures, this adjustment shall be carried out immediately by the Parties. These individual measures are set out in Appendix 1 to this agreement. 6 Amendment, blocking, and deletion of data The Contractor may only amend, delete, or block data that is processed during the assignment if instructed to do so by the Client. Should affected individuals contact the Contractor directly and request the amendment or deletion of their data, the Contractor shall immediately report these requests to the Client and, if necessary, support the Client in taking any measures required. 7 Deletion of data and return of data carriers At any time requested by the Client, but at the latest upon the expiry of the agreement, in accordance with data protection regulations, the Contractor shall return to the client or destroy with prior approval all documents, processing and usage results, and databases acquired in the course of the contractual relationship. The same applies to test and waste material. The deletion report must be submitted to the Client without being requested. Documentation demonstrating that data has been processed correctly and in accordance with the agreement must be retained by the Contractor for the appropriate period after the end of the agreement. For the Contractor's convenience, it may be handed over to the Client when the agreement ends. Page 8 of14
9 8 Damages Should the content of databases be disclosed to third parties, in breach of the confidentiality requirements, the Contractor must compensate the Client for any damages that may arise in accordance with the principal agreement. The Contractor shall be liable for the errors of its personnel in the same way that it is liable for its own errors. 9 Security interests Should the Client's property be put at risk by the Contractor through the actions of third parties (such as seizure or confiscation), bankruptcy or insolvency proceedings, or through other events, the Contractor must immediately inform the Client. 10 General 1) Should individual provisions of this agreement be invalid, this shall be without prejudice to the effectiveness of the remaining provisions of the agreement. 2) No ancillary verbal agreements may be made to this agreement. Any amendments or addenda must be made in written form in order to be valid. This shall also apply to the removal of this written form requirement. 3) This Contract is subject to German Law. UN private law and legislation on contracts for the sale of goods. The place of jurisdiction is Karlsruhe. However, Astaro reserves the right to take legal action against the Client at the court with jurisdiction over its registered office, as well...., on (date) (Client) (Contractor) Page 9 of14
10 Appendix I: General technical and organizational measures Private bodies which collect, process, or use personal data themselves or on behalf of a third party must implement the necessary technical and organizational measures to ensure compliance with EU data protection regulations. Measures are only required if the effort involved is in proportion to the protection required. The law defines various types of control that enable legal requirements to be met. These shall be implemented as follows by the Contractor: 1) Access control Access to data processing equipment with which personal data is (or may be) processed or used should be denied to unauthorized persons. The Contractor must therefore ensure that unauthorized persons cannot access areas in which the Client's data is processed or saved and cannot view or access data processing equipment (monitors, printers, and so on) used to process or display the Client's data. Measures taken by the Contactor in order to comply with this obligation: The operator's building is accessed through a door in the entrance hall which can only be opened using a transponder and a PIN. Images of everyone who accesses this room will be captured on video camera. The images from this camera will be recorded and saved for a limited period. Likewise, individual server rooms can only be accessed by specially authorized individuals using a transponder and PIN code. If data is processed at external data processing facilities, access control (such as backup) is provided by means of ID checks and secure access zones protected using code cards and PINs. 2) Access control Unauthorized individuals must not be permitted to access the Contractor's data processing systems. The Contractor must therefore provide individuals employed to carry out the services specified in the principal agreement with a secure user ID. Measures taken by the Contactor in order to comply with this obligation: Access to the computing center resources is restricted through a strict domain access policy. Identification and authentication at the domains is provided through a username and a complex individual password which must be changed regularly. Failed Page 10 of14
11 unauthorized access attempts will be recorded and monitored. If the user is absent, the system is automatically locked. 3) Access control It should be ensured that only individuals working to fulfill the principal contract are able to access the Client's data. In addition, appropriate access rights should be established (profiles, groups, roles, and so on). These rights should be granted purely on a need-toknow basis. Measures taken by the Contactor in order to comply with this obligation: Rights are only granted after approval and purely on a need-to-know basis. As far as technology allows, personal accounts will be used for administration. Every user account is therefore assigned to just one person. Rights are allocated according to roles or, as recommended by Microsoft, nested within resource and authorization groups. This makes both changes to rights and regular monitoring easier. 4) Transfer control The Contractor must ensure that the Client's personal data is not read, copied, altered, or removed by unauthorized individuals during its electronic transfer, transport, or storage on data carriers. Personal data may only be transferred and saved via in encrypted form. Personal data may only be saved, sent, or transported (such as when the data is issued by the Client) on mobile data carriers (DCs, USB sticks, disks, memory cards, and so on) in encrypted form. During remote maintenance or service procedures, the Client's data may only be accessed via secure, encrypted connections. The secure transfer modes and encryption methods should be regularly updated according to the recommendations in the data protection manual issued by the BSI (Federal Office for Information Security). Measures taken by the Contactor in order to comply with this obligation: Data transferred by the client is transported and saved in encrypted form. The relevant areas of the data carriers are encrypted using data and hard drive encryption Page 11 of14
12 software. Only encryption procedures considered secure by the BSI are used to do this (such as AES256). 5) Input control The Client must ensure that it is possible to verify and establish at a later date whether and by whom personal data was input, edited, or removed from the data processing system. Appropriate reporting systems must therefore be in place for these activities. Measures taken by the Contactor in order to comply with this obligation: The Contractor uses reporting systems that record and safeguard data access, storage, and data protection. 6) Order control The Contractor must ensure that the Client's personal data is only processed according to the Client's instructions. If the Contractor employs a subcontractor, the subcontractor must be obliged to follow the instructions and comply with the data protection requirements in the same way as the Contractor. Measures taken by the Contactor in order to comply with this obligation: Appropriate processing and change processes have been established to ensure that customer orders are handled correctly. Changes are only carried out in a controlled procedure and with notification from the Contractor. Page 12 of14
13 7) Availability control The Contractor must ensure that the Client's personal data cannot be lost or destroyed. This requires the following safety measures, in particular: - Implementation of appropriate data protection methods such as tape backup, data mirroring, snapshots, and so on. - Physical separation of the backup data. - The use of uninterrupted power supplies to ensure that data is not lost during storage or transfer. - Implementation of security measures that prevent access by unauthorized third parties (virus protection, firewall, spyware detection, packet filters, and DMZs, for example). - The creation of emergency plans. Specific measures taken by the Contactor in order to comply with this obligation: In order to provide the highest possible levels of availability, the Contractor uses a variety of technologies and processes. So that data can be restored in an emergency, data is regularly backed up in archive networks in different geographical locations to the storage centers. Data stored in the archive is always saved using redundant systems. To ensure an uninterrupted supply of power to the system, redundant power supply units are built into the systems wherever possible. The power supplied to the systems is backed up using uninterrupted power supplies. Security measures are in place to prevent intrusion from unauthorized third parties. All links to unsafe networks (such as the internet) are protected using firewall systems. The relevant security measures are regularly updated in line with developments in technology. 8) Separation The Client must ensure that data extracted for different purposes is also processed separately. The data must be separated in such a way that it cannot be confused with data belonging to other contractual partners or clients of the Contractor or be accessed by unauthorized third parties (even unintentionally). Page 13 of14
14 Should data belonging to other contractual partners or clients of the Contractor be accessed or confiscated by the authorities, it must be ensured that the Client's data is not affected. The Client's data may not be used for test purposes. Measures taken by the Contactor in order to comply with this obligation: To separate network traffic created by different clients, all data is encrypted using an encryption certificate that is unique to each client. It is therefore not possible to access data belonging to one client directly from data belonging to another client. Furthermore, the encryption certificates are only kept in the main memory of the processing system during the processing period and cannot be viewed by third parties. Page 14 of14
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text:
It was so touching, when the mother gorilla decided to show off her baby to the crowd hugging and kissing him warmly
An incredible scene in the zoo shocked the whole world. The mother gorilla approached the crowd and introduced her baby. It was such a sweet moment when
The orphaned gorilla hugs his carer everytime he sees him, as if wanting to express his endless gratitude for everything he does for him
This is a very precious moment, which undoubtedly deserves the attention of all of us. The photo you see on your screens has a very exciting backstory. There'
The sweet gorilla started showing off her baby to visitors, then showering him with warm kisses
What happened seemed so unbelievable. That scene will remain forever in everyone's memory. The mother gorilla suddenly started showing her baby to
It was such a touching moment when the man started consoling the poor gorilla who just lost his mom
Recently, a heart-wrenching photo began to spread quickly on the whole Internet, which attracted the attention of many people from all over the world.
The mass-rescued gorilla has an incredible friendship with a small wild bush baby
There have been several incredible couples over the years, and those that include wildlife are truly breathtaking. Take, for example, the companionship
The gorilla reunited with the person who raised him five years later
Many of you may have heard of John Damian about Androcles Aspinal. He is an English multimillionaire, a great lover of animals, especially gorillas.
Gorilla mama saves 3-year-old boy
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redpajama_set_name: RedPajamaCommonCrawl


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I've been working on my Bobble Sheep Pillow pattern for several weeks now, and I am very close to being able to publish it here on the blog! About a week ago, I finally finished the first sheep, and I love how it turned out. Excuse the awkward face I'm making in this photo–I still don't know how to model my makes without making strange faces!
I knew from the start that I wanted to have two sheep pillows: a creamy white sheep and a beautiful black sheep. After I finished the first sheep, the second one went pretty quickly–until I ran out of yarn!
The main problem I had was that you can kind of see the pillow behind the bobble stitches. This is hard to avoid, given the size of the hook and yarn that is used. It didn't matter for the white sheep, but the black sheep made the white pillow inside much more noticeable.
The solution? Make a pillowcase!
Now, I've designed these Bobble Sheep Pillows so that you don't have to add a zipper. The outer bobble casing completely encloses the pillow inside, so my pillowcase didn't have to be pretty.
Which is a good thing, because mine turned out ugggggly.
Even though I have a pretty decent entry-level sewing machine and some rudimentary sewing skills, I really didn't have the patience to try and set it all up. So, naturally, I grabbed my Skip-Stitch rotary cutter, some fleece remnants, and some chocolate to keep me going!
If you want a proper tutorial on how to make a circle pillow case, I recommend this one. If you're at all interested in how I manhandled some fleece into a pillowcase, read on!
This is not really a tutorial on how to make a pillowcase. But if you're in a bind, this method will work okay-ish! It'd work even better if you can cut even circles and are better at measuring than I am!
To start, I grabbed some black fleece from my collection of fabric scraps. This was leftovers from backing my Star Trek lapghan! Yay for recycling!
Next, I moved the pillow aside and laid my fleece "hourglass" out flat on my cutting surface. You can see in the photo that my cutting was terrible…I'd like to blame it on the dull scissors, but most of the problem was my impatience!
Using my Skip-Stitch rotary cutter, I unskillfully carefully made some cuts about half an inch from the outer edge. This fleece was pretty thick, so I had to press my cutter pretty hard against the fleece in order to cut all the way through.
After I'd gone all the way around, I folded the fleece in half again and inserted a size G crochet hook through the holes in BOTH sides of the fabric at one end of the pillow case. Then, using worsted-weight yarn in black, I pulled up a loop and continued to crochet through the holes around the edge of the pillowcase.
I'll admit, it was not pretty. But I continued in this method until I was about halfway around the pillowcase. Then I inserted my pillow and continued to crochet around the last half of the pillowcase, fastening off at the end.
Not pretty, am I right? I barely even wove in the tail ends of my yarn. Who's going to see this pillowcase outside of the pillow? No one, that's who! But after I put this pillow inside the outer bobble stitch casing, you can't even tell!
And, most importantly, you can't see any white bits of the pillow poking through the bobble stitches. Mission accomplished!
I've finished the head for this Bobble Sheep Pillow, but I haven't had a chance to sew it on yet. I am planning to do that tonight. Then I'll snap some much better photos than the one at the top of this post and get the pattern posted tomorrow!
I love the white sheep and awesome idea on the pillow case. So looking forward to your next post! BTW! What is your chocolate go to? Ferro Rocher has helped me through some serious Entrelac noodling!
Oh, I have SO many chocolates that I love! But if I'm having a super stressful day, I always choose Lindor chocolate truffles. Yum yum!


text:
The Devil's Advocate • March 30, 2021 • https://www.ridgedevilsadvocate.com/sports/2021/03/30/extra-runners-in-extra-innings/
Extra Runners in Extra Innings?
Evan Woo '22
One of the MLB's most controversial rules will be back in 2021. Recently, it was announced that the MLB and the MLBPA agreed to once again start all extra innings with a runner on second base [1]. The rule, introduced last year to prevent long games in a schedule that was jam-packed due to the delay from the pandemic, sparked much debate from players and fans alike.
Many fans felt that it artificially brought games to an end when neither team had earned a victory, and many pitchers voiced their displeasure with the fact that they would be essentially set up to fail in extra innings through no fault of their own. Others thought it would result in mundane endings to exciting games, with bunts and sac flies enough to steal a win. In Spring Training 2020, numerous Yankees pitchers commented on the situation. Said reliever Chad Green, "I don't know who wants to pitch with a runner on second with nobody out in an extra inning game. It's not something that you necessarily look forward to when you're about to run out there." Adam Ottavino put it bluntly. "It's just not real baseball." [2] Despite the rules unpopularity, the league went forward with it as part of their "2020 only" rule changes .
For those opposed to the rule, their nagging suspicion about the rule appears to have come true – presenting the rule as a 2020 only exception was just a way to introduce it to the game. However, in 2020 the rule served its intended purpose surprisingly well. 92 percent of extra inning games in 2020 ended by the end of the second extra inning, compared to 69 percent in 2018-2019 [3]. Because of its effectiveness in keeping games to a reasonable length, some around the league who were initially opposed warmed up to the rule. Dodgers manager Dave Roberts, who had mixed feelings on the rule going into the season, said afterward that he sees how "it could still be a benefit going forward." [3] Diamondbacks catcher Stephen Vogt explained that he sees the rule as a necessity for keeping games short in a world where Covid can upend the schedule and result in lost off days later on down the road. "I would love to get back to traditional baseball ASAP, but I like them for the 2021 season", he explained, continuing the familiar mantra of "it's just one season"[4]. Whether the rule survives the 2022 collective bargaining agreement remains to be seen, but like it or not, it will be a part of the game in 2021.
https://www.bleachernation.com/cubs/2021/01/15/the-runner-on-second-base-rule-and-7-inning-doubleheaders-are-expected-to-return-in-2021/
https://www.newsday.com/sports/baseball/yankees/yankees-extra-inning-rule-2020-adam-ottavino-1.47331931#:~:text="It's%20just%20not%20real%20baseball,rule%2C%20to%20be%20honest."&text="I%20don't%20know%20who,in%20an%20extra%2Dinning%20game.
[3] https://www.truebluela.com/2021/2/8/22273366/mlb-rule-extra-innings-second-base-doubleheaders-seven-innings
https://www.foxsports.com/midwest/story/mlb-runner-second-base-seven-inning-doubleheaders-2021-020821


This interesting portrait features a Regency lady in a garden setting, her arm resting on the base of a rose-entwined stone column. She is wearing a blue empire-line dress edged with lace with a white tucker fill-in. She has hooped pearl earrings and a pearl necklace; her dark hair has been curled and swept up.
What makes this portrait particularly interesting is that, in addition to being signed – J. W. Childe / 1817 – in the bottom left corner, is that it also has a preparatory pencil sketch of the same lady on the reverse side together with a fuller signature – J.W. Childe Pinxt / 1817 / 125 Strand / London.
The portrait is painted in watercolour on card and is presented in a decorative giltwood frame that is also glazed on the reverse to reveal the sketch with the inked signature.


text:
Application of polymeric microfluidic devices for separation of single-stranded DNA
Shawn Llopis, Louisiana State University and Agricultural and Mechanical CollegeFollow
Microsystems targeted for DNA sequencing, especially those focused on electrophoretic separations, are rapidly proving their viability to genomic research, mimicking the progress made when capillary electrophoresis developed from miniaturizing slab gel electrophoresis techniques. Being the more recent electrophoretic separation platform, the commercial availability of microchip electrophoresis devices remains relatively limited. To this extent, high-aspect ratio microstructures formed in thermo plastics have been developed using rapid fabrication methods from molding tools designed for mass replication of high-aspect ratio microfeatures. In this work, the choice and compatibility of poly(methylmethacrylate) (PMMA) – the primary substrate for DNA separations in this work – was investigated for use with our fluorescence lifetime detection instrument. The accuracy and precision of the fluorescence lifetime values of dye-labeled primers used for construction of single-stranded DNA (ssDNA) sequencing tracts was determined to discern the influence of PMMA as a substrate material to the discrimination method. The separation performance of ssDNA was evaluated for potential use of the polymer-based microchip electrophoresis devices as a platform for rapid, high-throughput DNA sequencing. To enhance these separations, a scheme to modify the surface of PMMA employing chemical and photochemical methods was developed. Once optimized, a linear polyacrylamide-modified PMMA surface demonstrated an electroosmotic flow, which varied from chip to chip, lowered by two orders of magnitude and demonstrated increased efficiencies for the separation of ssDNA fragments. As part of a modular system for the analysis of DNA material being developed in our labs, a purification device fabricated in polycarbonate was used to reversibly immobilize DNA sequencing fragments. The purified ssDNA was collected and analyzed by capillary electrophoresis to evaluate the device's efficiency in removal of contaminants from fragments constructed with dye-labeled primers. One significant result showed the necessity for a down-stream concentration method. Thus, we have investigated the use of a thermally responsive polymer, poly-N-isopropylacrylamide (pNIPAAm) grafted onto the surface of PMMA to serve as a concentration medium for the purified fragments. Results suggest pNIPAAm will be effective in concentrating and releasing fragments when changing the temperature from above its critical temperature (32°), where it exhibits a hydrophobic nature, to below it where it becomes hydrophilic.
Llopis, Shawn, "Application of polymeric microfluidic devices for separation of single-stranded DNA" (2006). LSU Doctoral Dissertations. 2064.
Steven Soper
Chemistry Commons


text:
'They Think You're a Joke': RNC Turns Viral CNN Segment Into New Pro-Trump Ad
Lawrence Richard
The Republican Party is turning a viral CNN segment into a new pro-Trump campaign ad, encouraging Republican voters to turn out with big numbers in November to re-elect President Trump.
The new ad, called "They Think You're A Joke," is centered around a recently aired segment with CNN host Don Lemon and his panel of guests mocking Trump and his supporters as illiterate and uneducated.
Anti-Trump "conservative" Rick Wilson and CNN contributor Wajahat Ali joined Lemon on the segment and are featured in the new ad. Hillary Clinton, Joe Biden, and Pete Buttigieg are also in the new ad, with their own criticisms of Trump's supporters.
BizPac Review reports:
The clip began with 54-year-old Wilson mocking the president's alleged lack of intelligence and labeling his base the "incredulous boomer rube demo" …
His condescending remarks were then contrasted alongside the equally condescending "deplorable" remarks of failed 2016 presidential nominee Hillary Clinton.
Also included were clips of:
Current Democrat candidates Pete Buttigieg and Joe Biden labeling the president's supporters racists.
MSNBC's Chris Matthews describing Trump's base as "low information."
Ali suggesting Trump voters don't know basic geography.
Wilson suggesting Trump voters can't perform basic math or read.
Throughout the whole segment, Lemon laughed and laughed and laughed.
Don Lemon, CNN & the rest of the leftwing elites aren't even hiding the hatred they have for Trump supporters anymore. They smear us, mock us & belittle us because they still can't accept that Crooked H lost in 2016.
Lets send the liberal elites a LOUD message in Nov! #Trump2020 pic.twitter.com/cpQH9EFZ68
— Donald Trump Jr. (@DonaldJTrumpJr) January 29, 2020
The new ad looks similar to a previous ad the Republican Party put forth highlighting "the extremist ideologies that have taken over the Democrat party, which threaten the freedoms of all Americans," the Daily Wire reports.
The ad features, per the report:
Audio of news reports talking about ANTIFA violence.
Alexandria Ocasio-Cortez saying the world will end in 12 years.
Beto O'Rourke saying that Democrats are going to confiscate American's guns.
Elizabeth Warren saying she wants to eliminate cars and electricity.
Bernie Sanders saying that "you cannot go too far" on pushing radical climate agendas.
Joe Biden saying he wants to shut down coal-burning plants
Bernie Sanders saying it's a good thing when people line up in bread lines
Beto O'Rourke saying he will have law enforcement go to people's homes to confiscate guns.
Joe Biden agreeing that "a Biden administration means they're going to come for my guns."
Ted Lieu saying "I would love to be able to regulate the content of speech, but the First Amendment prevents me from doing so."
Beto O'Rourke agreeing with the statement, "religious institutions should lose tax-exempt status if they oppose same-sex marriage."
Or, yet another, which shows various members of the Democrat Party speaking about impeachment.
New RNC ad uses Democrats' own words on impeachment against them pic.twitter.com/GDm8xgJ0wR
— Ryan Saavedra (@RealSaavedra) November 21, 2019


Walk-in Examination every FRIDAY at 9:00 AM, except holidays at the CMU Office of Admissions, Scholarships, and Placement.
The said exam is intended for the Second Semester SY 2016-2017.
Note: Foreign students can take the examination ——— Monday to Friday at 9AM & 2PM.
Walk-in Monday to Friday at 9:00 AM, except holidays at the CMU Office of Admissions, Scholarships, and Placement until the end MAY 2016 only.
Walk-in Monday to Friday at 9:00 AM and 2PM, except holidays at the CMU Office of Admissions, Scholarships, and Placement.


text:
using System;
using System.CodeDom.Compiler;
using System.ComponentModel;
using System.Diagnostics;
using System.IO;
using System.ServiceModel;

namespace Alchemy4Tridion.Plugins.Clients.CoreService
{
	public interface IUploadRequest
	{

	}
}

redpajama_set_name: RedPajamaGithub


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In general, the resistance of carbon steel and other alloys to High temperature corrosion is determined by the ________ content of the material.
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As with the case of the picture-winged fly, this is one of those small arthropods that are so common and humble, I've never bothered to learn about them. But now I'm glad to have looked them up!
So today I'd like to introduce you to Tetragnatha! I'm pretty sure the species is T. elongata (though I'm not ruling out the similar T. extensa—apparently there's a lot of variability in the drab coloration and patterning in both species). Also, the photos on this post show three different individual Tegragnathans, and they might not all be the same species, anyway.
Pronunciation: Do you suppose it's pronounced TET-rag-NATH-ah, or TET-rah-NATH-ah? Gnathos is the Greek word for "jaw," and I gather the g is silent—but then I was taught that the group of "jawless fishes" (including the lampreys), Agnatha, is pronounced "ag-NATH-ah," with a hard g. If anyone knows how to say this, please chime in!
Some folks call these "stretch spiders," because when at rest, or when frightened, they strike a uniquely "elongated" or "extended" pose, with the two long, skinny front legs stretched straight out before them, and the hindmost pair stretched behind. With their drab tan or gray costumes and narrow bodies, they look like an insignificant little twig or piece of grass.
The third pair of legs are shorter than the other pairs, and these are used to grasp whatever they're resting on—a cattail blade, the underside of a dock railing, or their web.
Now, most spiders that spin orb webs (you know—those beautiful, intricate wheels of gossamer to net their prey with) make them "vertical"—they're upright, like the wheel of your car, when it's on your car.
Well, Tetragnatha seems to usually spin its web horizontally—parallel to the ground—like a tractor tire that's being used as the boundary of a child's sandbox.
But Tetragnatha's web is not usually positioned over the ground. You'll find their webs most often over water—lakes, ponds, streams. I see them commonly on the edges of docks, where a right-angle bend makes a nice V-shaped nook for them to build in.
Now, why in the world do they position their webs like this? Are they trying to catch jumping minnows—? No.
At this point, it's time to talk about the life history of the mayfly! Mayflies, like many insects, undergo metamorphosis from unwinged juveniles to winged adults.
And the juveniles of mayflies (and dragonflies, too) are aquatic. Without the wings, they are odd-looking armored, legged insects with antennae-like "tails" that creep around on the bottoms of lakes and ponds, acting like "bottom feeders," for the months and even years it takes for them to mature.
So the mayflies say goodbye to their murky aquatic existence, crawl out of the water, and transform into their equivalent of "butterflies"—delicate insects capable of flying through the bright, clear air. It's just like when cicadas emerge from years of living in the soil to be reborn as glorious flying, singing creatures.
But right over the spot where the mayfly's wings are just now hardening, in the morning sun, into the machinery of flight, guess who's cast her big hunting net? Yep. Tetragnatha. The mayfly opens its wings a few times, stretching its newly discovered flying muscles, then leaps into the air for its very first flight . . .
Now, now—don't be crying over the mayflies! They are famously numerous, sometimes occurring in swarms. Some people absolutely despise them and the greasy mess their swarms make. In their adult form, mayflies are also famously short-lived (the name of their order is Ephemeroptera—the ephermeral insects). And anyway, if you eat meat and eggs, then you can't talk—cows, pigs, chickens, lambs, all have more "going on upstairs" than the pin-headed mayfly.
By the way, another common name for spiders in this family is "long-jawed orb weaver." The genus name Tetragnatha itself means "four-jawed spider"—which although literally untrue (they have just two fangs like all the other spiders), does colorfully describe the fact that the fangs are much longer than those of many other spiders!
In taking my pictures, I didn't go so far as to harass (much less kill) any of these spiders just to show you the full extent of their unfolded fangs (chelicerae—chuh-LISS-er-ee)—but if you search the "Web" (ha ha) for pictures, you'll find close-ups of the face, of the fangs, and even of the fangs "in action": When a male and female Tetragnatha "get together," they lock chelicerae. They, too, are "pin-headed." The nice long "arms" of the fangs hold off the hungry female, preventing her from devouring the male before his "mission" is complete.
Sooooo . . . the next time you're on a dock, or walking along the edge of a lake hunting for a nice fishin' spot, or even taking a walk to the "island" at Columbia's wonderful Stephens Lake Park (where many of these pictures were taken), keep an eye out for skinny little Tetragnatha, and greet her by name.
Great photos and a great read! I enjoyed learning more about this spider and the mayflies as well! Thanks for sharing this info!
Thanks, Gretchen, for your kind comment. I might have gotten better pictures of the spider on the railing if I had poked and prodded her, but I found her on a chilly morning after a day of rain, so I decided to give her a break!
On mayflies: you might enjoy a recent post I did on the natural history writer Edwin Way Teale and his book "Journey into Summer." In it he describes the huge swarms of mayflies over Lake Erie in northern Ohio--an annual event. Here's the URL: http://opulentopossum.blogspot.com/2011/07/edwin-way-teales-journey-into-summer.html.


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Q: Passing array as parameter in filter function with spring-data-mongodb I have this collection of documents:
[
    {
        "name": "name1",
        "data": [
            {
                "numbers": ["1","2","3"]
            }
        ]
    },
    {
        "name": "name2",
        "data": [
            {
                "numbers": ["2","5","3"]
            }
        ]
    },
    {
        "name": "name3",
        "data": [
            {
                "numbers": ["1","5","2"]
            }
        ]
    },
    {
        "name": "name4",
        "data": [
            {
                "numbers": ["1","4","3"]
            }
        ]
    },
    {
        "name": "name5",
        "data": [
            {
                "numbers": ["1","2"]
            }
        ]
    }
]

I want to get all documents of this collection when an array passed as a parameter is a subset of data.numbers.
This is the aggregation that I'm using.
db.testing.aggregate(
    [
        { "$match" : { "data.numbers" : { "$exists" : true } } },
        { "$project" : { "is_subset" : { "$filter" : { "input" : "$data", "as" : "d", "cond" : { "$setIsSubset" :[ ["1"],"$$d.numbers"] } } } } },
        { "$match" : { "is_subset.0" : { "$exists" : true } } }]
);

I'm trying to reproduce the above aggregation in Spring Data MongoDB.
How to pass an array as parameter in $filter and $setIsSubset functions?
 operations.aggregate(
                    newAggregation(Testing.class,
                            match(where("data.numbers").exists(true)),
                            project().and(
                                    filter("data")
                                            .as("d")
                                            .by(???))
                                    .as("is_subset"),
                            match(where("is_subset.0").exists(true))
                    ), Testing.class);

A: I solve my issue.
operations.aggregate(
                newAggregation(Testing.class,
                        match(where("data.numbers").exists(true)),
                        project("id", "name").and(
                                filter("data")
                                        .as("d")
                                        .by(context -> new Document("$setIsSubset", Arrays.asList(numbers, "$$d.numbers"))))
                                .as("is_subset"),
                        match(where("is_subset.0").exists(true))
                ), Testing.class);

I created a Document with the content that I needed in the $filter condition.
new Document("$setIsSubset", Arrays.asList(numbers, "$$d.numbers"))

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