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Arc flash protective faceshields are critically important in keeping electrical workers safe in low voltage environments where workers are required to wear ArcSafe Apparel and ArcSafe faceshields. Arc flash accidents can cause serious injuries or kill. The casualties resulting from these accidents are almost always devastating to the worker involved and to the worker's family.
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I've seen this tag flying around the place lately and I've just decided to join in.
Would you rather lose all of your mascaras, eyeliners, lipsticks and lip glosses or lose all of your palettes and eye shadows?
I don't use skin make-up are eye shadows so this one is pretty easy. I would keep the mascaras, eyeliners, lipsticks and lip glosses, just because I feel like your everyday make-up doesn't need to be too eccentric so bold, glimmer-y eye shadows aren't, for me, a must have.
Ahh, this one is so hard! My hair is so long and the thought of cutting it all off kills me. However if I never got my hair cut again, split ends would make it look horrendous anyway. I'll go for cut it all off.
Coral has almost a tint of peach in it, I think it blends with the skin a lot better. But pink's common and cute, I think it really depends on the day, the events and my mood. But I'm going to be going for a coral cheek.
OK, I would choose clothes but make-up really does finish off a look. I think, if you're wearing a gorgeous outfit it could just draw attention from your face, unless you look like you just been dragged out of bed. Which I doubt would make a difference, because we're all beautiful.
I don't really see the difference in this one, either way you'd have very uncomfortable eyes or lips. I did know if it's really common to have peachy, pink eyeliners. But black (being the common eyeliner colour) lipsticks are possible. I think applying lipstick to your waterline or around the eyes would be: a) very difficult. b) dangerous. So maybe I'd get away with using eyeliner as a lip liner.
In the UK we don't have Sephora so I'd have to say MAC. When I recently went to Paris in April I went into Sephora and they are huge, full of lots of different brands and just utterly fantastic. If Sephora came to the UK, I'd switch the answer to Sephora.
I don't really use eye shadows, in fact I never use them. So I'd have the lip colour for the rest if my life. I'd definitely want it to be a nice colour, not too vibrant or silly.
Honestly, without hesitation, bright nails. I think that in the summer bright nails spice things up a little, get you in a holiday mood, ect. but even in the winter when every thing's all dark you can still be the one all cheery with your bright nails. I don't like a nail varnish that they say us navy, or purple, or red and it to turn out just to be a dull black.
Definitely give up my lip product (hopefully that's lip make-up, not lip balm). My mascara is my favourite, I love having my eyes stand out. But I couldn't give up my lip balm. I literally carry lip balm with me every where and feel lost with out it. So that question's harder than I thought.
For me, a ponytail is a neater option but I tend to find when I'm working, looking down, it tends to split and falls to my ears. That just looks awkward. I feel like a messy bun is comfy. I actually find it hard to put my hair in a messy bun, although its supposed to be messy it's still got to look decent. So I think I'll go with comfort, the messy bun.
Would you rather never being able to paint your nails again or never use lip gloss?
KEEP NAILS! You'll probably know how much I love my nail polishes and my nail art. I only wears lipsticks and lip balms not lip glosses, I feel they're sticky and don't give as much colour as a lipstick would. But maybe one day, I'll go back to them, we'll have to see.
Wow, shaving them off would be... Different. I would say sharpie them in everyday because I prefer to have eyebrows coloured in, but still have hairs other than just block colour eyebrows.


Although the Lake Mille Lacs area is famous for great Minnesota walleye fishing and its tourism, there is more substance and depth to the area than that. There are eight established communities surrounding the massive lake. Wahkon was the earliest settlement in the Mille Lacs area, beginning its town history in 1885. The communities around the lake exemplify small town living at its best. They have rich histories. Today, our unique communities have fostered a unique lifestyle. The Lake Mille Lacs area is a real community–a good, decent place to live, which makes it a wonderful place to visit.
According to oral traditions, the Ojibwe first lived on the Atlantic coast of North America. About 500 years ago, the ancestors of the Mille Lacs Band began migrating west. By the mid-1700s, the Ojibwe had established themselves in the region around Lake Mille Lacs in what is today Central Minnesota. But it wasn't long before the Mille Lacs Ojibwe's self-sufficient way of life was affected by a new presence in their homeland. Because of diseases and federal policies, by the end of the nineteenth century, only a few hundred Ojibwe remained on the Mille Lacs Reservation. Over the next century, the Mille Lacs Band struggled with poverty and despair. Today, the Mille Lacs Band becomes the first Indian tribe in the country to use casino revenues to back a development bond issue, which raises more than $20 million. The Band uses the money to begin rebuilding its reservation and strengthening its culture. Community improvements to date include 2 new schools, a new medical clinic, 2 new ceremonial buildings, 2 new community centers, water treatment facilities, improved roads and new homes.
The village of Malmo on the northeast shore of Lake Mille Lacs was named after Malmo, Sweden, in honor of the Swedish immigrants who settled there. Like all lake communities, Malmo prides itself on serving seasonal visitors. Two churches, gas stations, cafe, convenience store, a flower shop, a new upholstery facility, a recreational vehicle repair outlet, liquor store, laundromat, boat storage and repair service and nearby resorts and supper clubs are among the facilities available. Malmo Memorial Park offers picnic tables, sand-bottom swimming and a well-maintained public access site.
Cove, is east of Onamia on Highway 27 amid tamarack trees. Watch for maple syrup and public water access.
Wealthwood lies to the northwest, rich in natural beauty, services, and vacation alternatives, including a long curve of sandy beach, the Wealthwood State Forest, a rod and gun club, camping, Minnesota resorts, restaurants, groceries, nightclubs and various cottage industries.


"Venezuela, New Grenada, Equador, and the Guayanas"
This superb reproduction of the original hand-colored steel plate engraving, "Venezuela, New Grenada, Equador, and the Guayanas" (sic), was first published by the noted English cartographer, John Tallis, in his Illustrated Atlas and serves as a fine example of mid-19th century pictorial mapmaking. Generally considered to be the last of the great pictorial mapmakers, Tallis's maps are eagerly sought after by collectors.
Of particular interest here are the splendid vignettes of Fall of Bagota, Paramaribo and Chimborazo. Each is an example of an artistic quality no longer available in contemporary cartographical art.


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Q: Nodejs + db-mysql Segmentation Fault I don't know what causes it, but I have a Node app that keeps crashing. The console says Segmentation Fault, and it looks like it happens when two Mysql objects are instantiated (using db-mysql module), which becomes very common when 10+ users are using my site (I don't post the link to the app because I'm afraid the load would crash it ;) if it can be useful I'll post it).
Do you guys have any clue? My packages are up to date. Do you have a better package to use with Mysql (assuming it's where the problem lies)? Do you also encounter Segfault issues using Nodejs (I guess not, bcs stability is one of the main advantges of Node)?

A: I [think] I was definitely doing something wrong: cerating a new MySQL object and connecting to the DB every time I had a reaquest. Instead, I stored the MySQL object and run a single query for each... query. Working fine so far.

redpajama_set_name: RedPajamaStackExchange


This white paper is written for nance professionals who are considering investment in accounts payable (AP) automation software for Microsoft Dynamics® AX. It will discuss best practices, provide an overview of AX AP functionality, discuss software that can extend AX to deliver the desired best practices and provide an overview of the Kofax® AP AgilityTM accounts payable automation solution for AX.
The stakeholder engagement paradigm has changed. Not only are organizations communicating with customers, suppliers, partners, and other constituents through additional channels, but the dynamics of those communications have been altered. The relationship itself is gaining value and is increasingly dependent on immediacy, transparency, and bidirectional communication via the preferred medium.
At the same time, enterprises continue to focus efforts on improving the productivity of their line-of-business knowledge workers in order to reduce the time they spend on an activity and to improve the quality and reliability of the decisions they make. Because these activities and decisions play a key role in the stakeholder relationship and ultimate business outcomes, strategies to streamline communication processes and perfect key data exchanges are essential.
Organizations frequently turn to business process automation to reduce cycle time and error rates. However, many processes still result in a significant number of exception situations that require manual handling by line-of-business workers. In addition, exception resolution may require workers to reach back to customers or other stakeholders, essentially restarting the process from the beginning, resulting in frustration and inefficiency on all sides.
The solution is a tightly integrated system that extends between where the stakeholder request originates and where the decision-making systems begin. The system must be able to accurately extract critical data from documents submitted via multiple channels with minimal or no stakeholder intervention. Furthermore, it must be able to consistently perfect documents and data before they are entered into the proper systems of record for high-value/high-cost decision-making processes.
Every day, organizations receive high volumes of various types of documents from different channels that launch or continue internal business processes. Reviewing and interpreting these documents, entering data from them, and forwarding them on to others for further processing consumes company time and resources, which increases operating costs and negatively impacts customer and supplier responsiveness. A digital mailroom powered by enterprise-level capture and business process management (BPM) technology solves these problems by converting incoming documents—whether originating from mail, fax, email, multi-function peripherals (MFPs), web portals, or mobile devices and tablets—into structured electronic information that feeds multiple business processes and systems across the enterprise.
In almost every organization there are accounting, human resources, purchasing, contract management and customer service processes. In addition, there are vertical market-special processes, such as insurance claims processing, mortgage processing, electronic health records management, new bank account opening, case management in government, and so on. Business process management (BPM) and dynamic case management (DCM) are used to orchestrate and automate the complex sequence of steps required to complete these processes, and these processes frequently require the capture of incoming business documents as part of the process.
Smart capture solutions capture and streamline the flow of business critical information throughout an organization in a more accurate, timely and cost effective manner. This white paper overviews how Smart capture can transform traditional incoming document reception and processing into an efficient "digital mailroom" that helps organizations reduce costs, drive process efficiency, and improve customer service and market competitiveness to better grow their businesses.
as an important step toward a new DMS: a document management system for the administration, preservation, storage and retrieval of documents within the organization.
According to Bellekom, the new DMS is part of the broad front of ce that municipalities currently have or plan to build. The front of the organization is composed of a website with electronic forms, a call center (Client Contact Center) and a mailroom where, in the case of Gouda, about 140,000 items come in annually.
The items have to be sorted and opened regardless," said Bellekorn. "They might be complaints, letters, application forms or opinions. Employees read and assess the contents, then register and classify the content using their experience, thereby setting the processes of the organization in motion. That work can be automated. For example, we already have experience with the digitalization of incoming invoices and Social Services documents. This expertise can now be used with the implementation of the digital mailroom.
While increasing sales is a natural goal for improving the bottom line, significant revenue can be found by optimizing AP automation. Improving workflows at every key milestone reduces costs of manual intervention, all while increasing early payment discounts. Below are ways your organization can implement best-in-class AP practices.
A modern financial system is the foundation for automation. Complete accounts payable automation solutions are a relatively new product category for financial operations professionals. Advanced technologies such as mobile, social networking, and intelligent dashboards are very slowly being integrated into offerings and adopted by enterprises.
Finance professionals are cautious by nature and do not always view AP automation solutions as being strategic to an enterprise. However, in the past 10 to 15 years, companies have automated components of their AP process, doing it in phases with a slow adoption rate.
Let's look at the current phases for incorporating an automated solution into AP systems, as well as the current state of adoption, and predictions about where the industry is headed.
Electronic signatures, or e-signatures, have become a standard part of many business processes today. When deployed across mobile and traditional devices, e-signature technology significantly improves your organization's efficiency resulting in a better customer experience.
In this three-part blog series, we'll discuss how deploying e-signature technology across your entire organisation benefits your bottom line by boosting productivity, driving customer engagement and enriching the customer experience with omni-channel availability.
While the term "operational excellence" can vary in meaning depending on the organisation, when businesses gather metrics from key performance indicators (KPIs) to their descriptions of operational excellence, real progress is made. Whatever the definition, the most-cited goal of achieving operational excellence is to exceed customers' expectations with every transaction, every day.
As management guru Peter F. Drucker asserted, "The purpose of business is to create and keep a customer." E-signature technology can help your organisation become more efficient with friction-free, fluid processes that enhance the customer experience, enabling you to create and keep them. The right e-signature technology improves the operational efficiency of your organization in part because of the ubiquity of transactions, which require signatures.
From onboarding a new customer and opening an account to completing a loan application and more, signatures are required to complete many transactions. The signature captures your customers' intent and signifies that they have agreed to the presented terms and conditions. Customers accept the need for the process and value the implied security, but there is no denying that the traditional paper-based process can be cumbersome. In addition to delivering a less than ideal customer experience, paper-based processes slow down business, costing time and productivity.
Faster customer acquisition: Workflows streamlined by the adoption of e-signature technology result in a more efficient customer turnaround time and reduce the risk of cancellation.
Cost reduction: Implementing a paperless process reduces the following expenditures: cost of paper products, courier and shipping charges and storage.
Straight-through processing: The 100 percent digital process removes the time-consuming task of re-keying information and eliminates data entry errors.
Easy retrieval and archiving: E-signed documents are archived automatically and retrieved easily when needed.
Customer-friendly process: Electronically signing on a mobile device such as a smartphone or tablet offers an easy and technically familiar process that customers trust.
E-signature technology helps your organization become more efficient with friction-free, fluid processes, enriching the customer experience and increasing customer retention.
More companies, across a variety of industries are embracing e-signature technology as an opportunity to transform the customer experience. However, in a move to reduce operating costs, too many organizations are relying heavily on automated interactions. But reducing or obscuring in-person transactions can degrade the sales experience, sometimes even prompting customers to move to a competitor. Use digital to augment – not only replace – your face-to-face transactions.
How do you "go digital" without losing the personal touch? Leveraging e-signature technology makes digital transactions personal, and serves your clients anywhere, anytime, and on their preferred device.
Business customers are savvier than ever before and expect certain transparency at each stage of the buying cycle and beyond.
1. Ease of Use – Select an e-signature solution that allows your customers to use self-service technology at their own pace, such as allowing them to review documents before signing or completing the workflow without installing additional software.
2. Device-friendly – Customers expect transactions to be secure and accessible from any web-enabled device (computer, tablet or smartphone) at any time.
3. Security first – E-signing isn't new. Customers should find it natural to transition to a digital process. However, organisations must be able to create tamper-evident documents. Being able to produce an audit trail of electronic evidence that demonstrates the signer understood and accepted contract conditions at the time of e-signing, builds trust with customers. And improves your sales efficiency.
4. More interactive assisted process –Using in-branch devices such as tablets, that can be handed back and forth with the customer is an example of a collaborative transactional process. It's a guiding example of ways leading e-signature technology can deepen and improve customer interactions.
5. "Traffic Light" Navigation – Digital transaction processes that include your employee or end customer to complete one step before they move to the next help to reinforce consistent sales interactions from sales and customer service teams.
Maximise sales effectiveness and maintain your competitive edge, enrich – don't just replace – in-person customer experiences with efficient digital processes.
Customers expect a seamless, consistent experience across all channels, supporting the case for implementing e-signature technology across your enterprise. Not only do e-signatures play a pivotal role in digital transaction management, they are also an important factor when delivering a unified omni-channel experience to customers.
Part of this comprehensive, omni-channel approach is e-signatures. Asking your customer to leave the digital world to sign with wet ink is like asking them to leave your brick-and-mortar location and come back another day to make a purchase – an irritating hurdle interrupting an otherwise smooth transaction. Enterprise-wide digital management technology avoids gaps in customer service and helps correct inconsistent or cumbersome processes.
The e-signing process and the user interface should be similar, whether the customer is signing online or in-branch.
Support for multiple signature types keeps the process digital. Offer your customers the ability to apply handwritten e-signatures on a mobile device or click "I Agree" to e-sign. In other words, remove friction from the process by providing a number of e-signing options.
If you have read this complete blog series, you are undoubtedly committed to building a better customer engagement model and questioning how to achieve optimal efficiency and sales effectiveness in this digital era. A personalized and consistent user experience is the foundation for your omni-channel strategy, and e-signature technology is the adhesive that keeps your process digital.
When you embark on an e-signature project, you'll have a long list of requirements. The following questions will likely be top of mind: Are the resulting e-signatures legal. Can I easily integrate the solution within my infrastructure? Do I need an on premise, or a cloud solution? These are all important considerations.
Validating an e-signature's integrity is critical, now and in the future. The ability to analyze a document and determine whether or not it has been tampered with, makes using e-signing technology arguably more secure than a traditional pen-on-paper process. If you can't perform that kind of analysis, the solution will eventually lose much of its value.
Before I dive into the importance of standards, let's step back, and explain the value of e-signing versus a paper process – the key is the ability to confirm the integrity of the document, at any time.
Applying a wet ink signature onto a piece of paper places a representation of your signature onto that document. There are no mechanisms in place to prevent someone from adding or modifying existing text within the document. The document itself can be easily tampered with after the signing takes place. Even the signature itself can be manually copied and placed at different locations within a document.
An analogous issue can be inherent in an electronic signing solution, too. Solutions that insert a pure image of your signature into a document – instead of a real standards-based electronic signature – suffer exactly the same disadvantages as the traditional paper-based process described above.
Specifically, the signature can be easily copied, tampered with, and reused and you have no way to detect this has occurred. Obviously, this defeats the purpose and is not desirable. What you need is a solution that is safer and more secure than the traditional paper process.
Optimally, an electronic signing solution should make it obvious the document was tampered with. If you are using e-signatures, but cannot guarantee the integrity of the document, you are missing one of the key benefits and advantages of using e-signing as opposed to wet ink paper signing (besides the obvious efficiency and cost benefits). Although most e-sign solutions guarantee the integrity of their signed documents, many don't guarantee it in a standards-based manner.
I'll argue that by relying on such solutions, you run the risk of being left with a database full of non-verifiable documents. This is because you will be dependent on your solution provider for signature validation. So how can we resolve this issue? To me the solution is obvious, the consequent use of standards for the electronic signatures that you place into valuable documents and contracts.
The ISO standard will solve your long-term e-sign validation issues. The Portable Document Format (PDF) is specified as a digital form for representing documents in the ISO 32000-1 standard. This format enables users to exchange and view electronic documents easily and reliably. The ISO 32000-1 standard identifies the ways in which an electronic signature, in the form of a digital signature, may be incorporated into a PDF document to validate the integrity of the document's content. The use of electronic signatures for PDF documents following ISO 32000-1 is specified in "Electronic Signatures and Infrastructures (ESI); PDF Advanced Electronic Signature Profiles" (ETSI TS 102 778).
Your e-signature solution should supports the ISO 32000-1 standard. As a result, any document e-signed using a handwritten, biometric, or click-to-sign signature, can be validated with any standard, compliant PDF Reader. Not only can your organization validate electronic signatures independently, but your own customers will also appreciate the ability to validate their copy of the e-signed PDF as well.
The use of proprietary software that locks you into one particular vendor is a thing of the past. Demand support for ISO 32000-1 and ensure your e-signed documents can be validated, anytime, without locking you into a solution – or, worst, locking you out of your e-signed documents.
Efficiency Leaders can help you transform your organisation with our range of superior products, innovative solutions and capability for seamless integration. Let's talk!


Soooooooo. Yeah. A reader asked me to write a post about the one thing I hate most about my body. While a few years ago I definitely would have answered with "my hair", I think I'm over that now. People sometimes encourage me to smile a little brighter on my blog, but I felt I always had a good reason not to. I hate my teeth. Even though a lot of people tell me they never even noticed it (seriously, it took my friend Susan 3 years to see it…), I am always aware of "The Gap". Whenever I have to laugh out loud, I'll place my hand in front of it, afraid to get more of those meant-funny-but-hurting jokes people made about it in the past. My family couldn't afford braces when I was younger, so throughout high school I was mocked with both my hair and teeth. A few years ago my mom went out working again and we finally went to see an orthodontist. She told me that I could get (invisi)braces and in two years the gap would be gone. But because I had a tooth pulled on my upper right side when I was 8, the gap would probably be back in no time. the orthodontist basically said that the only two options I have is to either get all my teeth pulled and wear fakes or wear braces for the rest of my life. Both not gonna happen, haha! Today I am learning to make peace with these two uglies, but I'm having a hard time. Maybe I should go to one of those laughing classes?
PS: please note that I know that there are more terrible things in this world than having a gap between your two front teeth. I myself have been through much worse things than this, like I'm sure many of you have as well. This blog is a place where I normally only share the good things that happen, because frankly, it's just no fun to share sad stories. Secondly, you know that thing people say when someone get's mad? Go to a happy place? For me one of those happy places is my blog. This blog means something to me and I sincerely hope no one will see this post as a way to get to me personally.
I think your smile is cute 🙂 I've actually started to notice that gap-toothed smiles are embraced rather than being perceived as a flaw. Lara Stone and Lindsey Wixston have similar smiles and they are loved for them.
i can't believe your dentist only gave those two options! i think your teeth look great. 🙂 also i can't believe you'd ever dislike your gorgeous red hair!
And I always think your photos are lovely – much more adventurous than my photos, I have the same pose every single day, haha. I should take tips from you!
I think the gap is cute…it adds character 🙂 I love your outfit, and I think these photos came out great!
How can you possibly dislike your hair? I love it! And the gap in your teeth is super cute. Really nothing to worry about!
Een spleetje tussen de tanden is als een statement, het maakt deel uit van wie je bent en dat hoef je niet te veranderen door dure (en misschien pijnlijke?) ingrepen. Je bent mooi zoals je bent en zelfvertrouwen hebben is het belangrijkste 😉 Het zijn hele leuke foto's!
Ps: Ik vind je haarkleur geweldig!
I know that feeling! Not with the gap, which I did have when I was younger, but being nervous about your smile. I grew up without doctors or dentists, and I took horrible care of my mouth, so I lost all of my teeth before I even turned 18. I got my first pair of dentures around 16-17 and they were made at home. :/ Because of that, and because they've been with me for a long time now (I only take them out to clean them), they don't fit right and holy hell do they hurt!!!
One day I hope I'll have enough to go to the dentist, get a good examine and replace these dentures with brand new, proper fitting ones!
Just beautiful! I think the gap adds character. Love it.
These photos are seriously cute. And I personally think the gap gives your smile a wonderful personality. I think that the things we dislike about ourselves are things other people don't notice or they can't imagine us without. Anyway, I think you are beautiful and you have beautiful hair and a beautiful smile!
Nikki, you are always inspiring to me! and you have a lovely smile! don't be too hard on yourself, I feel like a blog is a story and it changes as your life evolves. Taking photos and figuring out how to look and pose in them is a challenge for sure, but you do a wonderful job at it! I guess for myself what I'm learning is to try and take photos that enhance the things I like most about myself, or to share some emotion…or enhance the best features in others that I photograph. I love the background in these photos. I think your photos always have a creative, inspiring, genuine quality.
I want to steal this whole outfit!! You're adorable!
I think your teeth are adorable! I really like this look, too. That dress looks so comfy and that scarf so cozy. Great look for winter.
Leuke outfit! En de foto's waarop je lacht zijn heel leuk!
And yes…I agree that writing/sharing sad stories is not fun…we all have personal stories that are not so happy and to share them ALL the time would be less than fun. But, it's definitely a good reminder that we all have them.
I'm glad you've made peace with your hair because I think it's incredibly beautiful…and your smile too! But, of course, we all have those things that we hate about ourselves but which others never notice.
And I definitely need to get more creative with my poses as well – so this is like a little reminder to myself.
I finally just have to note that I'm terribly envious of how well you pull off a belted cardigan. I can never quite manage that.
Love you style. I think you have a gorgeous hair color and who cares what other people think right. It's your blog. I think you did lovely at posing. My poses are a bit boring.
Oh Nikki!!!I like so much this post about you!!!
One of my best friends has "the gap" too,but it is just a peculiarity that she only has!!!!So don't even try to hide it!
I'm also not very creative in posing, and I also have to admit, posing is not the most important thing for me: apart from that, the outfit matters, the writing and of course the person behind the blog, the personality. And there are many great and famous bloggers who (not saying that I'm able to pose) don't have many "different" poses, a blog should be like you say a little place where the blogger shares something of his life, something where the blogwriter feels comfortable with, and no competition. Just something to connect with people that have the same interests!
And what concerns your teeth: I can totally understand that (not saying that I would have noticed the gap without reading your post), but well, doesn't everybody have something about his/her body he/she doesn't like? For my part, it's a rather strange thing: I don't like to be photographed with my head turned to the left (aka to the right in photos), I really can't explain why, and nobody understands that, but I just don't feel comfortable with this side of my face.
And I think your outfit is pretty cute, I love this dress!
No no no! I got Invisalign here in America over 5 years ago. I corrected a horrible set of teeth and had one of the front bottom teeth removed. The invisalign fixed my teeth and I have a wire on the back of my remaining bottom teeth. I wear my clear invisalign trays every night (they are like very thin teeth whitening trays). I'm sad that your dentist didn't say he could correct your teeth. I am so much happier now and I love smiling when I laugh.
You look beautiful when you smile, don't ever let the gap stop you!
I have a larger than normal front tooth gap too, but really, no one notices as much as you do, and it's not worth being tight lipped over.
I'll admit that it really surprised me both to hear that you (at one time?) didn't like your hair because I genuinely find it Gorgeous. But then, being teased about physical things is so hard. I was also surprised to hear you had a gap in your teeth. I'm sure this doesn't really change how you feel about them, but looking at your photos here in this…. I think I rather like the gap. Like how really top models have physical things about them that are kinda quirky or different- not perfection- I think that's the gap. It adds character. Um, if that makes sense…. Okay, quitting rambling. Instead I'll just say that it is rather brave of you to come onto a place where people can comment and admit something like these insecurities. Good for you!
On a completely different note the very first thing I noticed about these pictures was how blue is just a fantastic color for you. Makes your skin look great and really brings out your eyes.
Hmmm… lastly, about the poses. I do a lot of silly poses too (leaning back on my heels, twirling, leaning down etc.) to expeirment. And some look really, really dumb. But its all exploring and learning. I also keep a pinterest board for photograph ideas and some pins are about poses. Though, I like the poses here just fine btw.
I think it's adorable and adds character. I too have messed up teeth, even after having braces for many many years. I think it's fine, who cares ya know?
and I love your hair color.
Golly, girl! I think you are so gorgeous and although we are are all hardest on yourselves I hope you can really take that in and love yourself, perceived flaws and all. Your photos are gorgeous, too! I live in a super populated and busy area and with it raining and getting dark so early it's extra hard to get good photos during the week. You seem to have access to an amazing backdrop for your photos. They are beautiful!
I think you have a beautiful smile. And that you are a strong woman for sharing one of your weaknesses with the world. I like to keep my blog a positive place too, and while I don't sugar coat things, I don't always talk about things that are bumps in the road – and I don't see anything wrong with that. I love your blog, and really enjoyed these pictures in this magical forest today!
You know, I never actually noticed you had a gap! I love smiles that have personality, and I think your gap just makes you even cuter 🙂 It's funny, I had braces when I was younger but because my teeth were so large and my mouth so small, one of my teeth moved back to being all crooked and turned, and my bottom teeth are super crooked! I used to be mad about it, but think it gives my smile personality now.
As for posing tips? I think I need those too, I always tend to do the same things!
I'm interested to see the outfit photo advice. I've steered away from outfit posts because I'm so awkward in front of a camera. I could definitely use some tips.
thanks for sharing! love the honesty, and love the outfit!
I actually like it when people have imperfect teeth-it gives their face more character! Perfect American teeth are soooo not for me!
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i think you look fabulous! i don't love my teeth much either because of a tooth that broke and I can't afford to get it fixed but I've found that bright lipstick helps a ton lol. Glad I found your blog!
I love your hair, the color is sooooo beautiful and your smile is great. I have one to it gives me character. Just keep being you, we like you as you are!!!
what a cutie! I want a scarf just like that. It's perfect with anything!
Oh my word, would never have given a second thought to your gap…look at Lara for heaven's sake 🙂 And love the dress you've got on, that crinkled texture fabric is really pretty.
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You are as cute as a button and the gap between the teeth?? isn't there a couple of famous models with gaps?
gosh Nikki, the backdrop in these snaps is awesome. i love the way it almost looks like a painting, how the trees are awash of fuzzy green and the burnt orange leaves trail back behind you. really charming and so are YOU lades!!! love the outfit.
you are so very pretty, i adore your soft and shiny ginger hair!! embracing the things we fixate on as faults is pretty tough stuff, we're always our own worst critics and man alive do i ever empathize with you on what a toll being made fun of in school takes on your own self image. eeps, for reals! your gap is something i never really noticed either, gotta say though, i think it's pretty darn cute! reminds me of Lauren Hutton right away not to mention Georgia Jagger who's fully embraced her gap with gusto.
i was made fun of in school for my braces (had them on for almost 3 years) and hardly ever smiled because of it. one day, at the back of class (cos that's where i'd sit to try and not draw attention) a boy who i thought was mighty hot turned around and said simply: "you should smile more, you've got a nice smile". it tripped me out, but from then on i consciously made more of an effort to smile and push the other harsh critics out of my mind. all it takes, sometimes, is one person to encourage you. i hope you've found that too because you are lovely in spirit and in beautiful presence.
LOVE this outfit! So cute! You have beautiful hair!
I love the gaps–they give your face a delightful personality! I don't think that you should change a thing. Many models love their gaps.I did want to mention that if you ever wanted to make a change, you wouldn't need braces forever, but would need to wear a "retainer" at night to have the teeth hold their positions. And, never would they pull the teeth! To make changes without orthodontia, they might do veneers (caps on the front) or add some composite material called "bonding" to fill in the spaces if you wanted (the easiest fix).


It's the end of a long summer of grilling. Now that you've had your fill of hamburgers, give salmon burgers a try! All it takes is a little cutting, measuring and mixing for them to be hot off the grill in less than 20-minutes!
[#image: /photos/57d8ebfe46d0cb351c8c7604]|||||| ____Then stir the dill into the Greek yogurt and season it with salt & fresh ground pepper. Set the spread aside for use later.
[#image: /photos/57d8ebfe50778cef321a6edd]|||||| ____Now it's time to get your hands a little dirty. The salmon needs to be cut into small cubes. I start by sharpening a long knife - if the knife is sharp it makes it much easier to cut the fish neatly. First I cut the salmon crosswise into thin strips.
[#image: /photos/57d8ebffd3276fe232948c80]|||||| ____Then I cut it lengthwise so that the fish ends up in small cubes (some pieces near the thicker end of the salmon fillet may need to be cut in half again to achieve an equal cube size).
[#image: /photos/57d8ec004b76f0f832a103fb]|||||| ____And mix it up so the ingredients are distributed evenly throughout the salmon.
[#image: /photos/57d8ec0150778cef321a6edf]|||||| ____Form the mixture into large patties and place them on a nonstick grill sheet which has also been sprayed with cooking spray or rubbed with a small amount of oil.
[#image: /photos/57d8ec014b76f0f832a103fc]|||||| ____Grill the salmon burgers over medium heat for about 6-8 minutes on each side. You have to do a low heat, slow cook for these burgers. If you cook them on too high a heat they will burn on the outside and be raw on the inside.
[#image: /photos/57d8ec0246d0cb351c8c7605]|||||| ____Enjoy the salmon burgers on a whole wheat bun which has been spread with the Greek yogurt and dill mixture!


Using exciting vectorized graphics, this highly kinetic games brings a whole new dimension to the tower defense. Devastate creeps with blasters, lasers and missiles and watch their energy debris swirl through the gravity wells of your vortex towers. Now it's time to figure out how to put that absorbed energy to good use….


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You can apply for The Organization of American States (OAS) Academic Scholarship Program for degrees studied at Marconi International University. The program grants undergraduate, graduate and master's scholarships every year to successful applicants belonging to the 35 independent states of the Americas for the pursuit of higher education studies.
Scholarships for Master's degrees
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The OAS scholarship will cover 60% of the total cost of tuition and is applicable only to the value of the program of study.
Other fees such as the payment of the initial fee, translation, and notarization or authentication of documents, study materials, procedures for obtaining the degree, and any other similar expenses are not covered by this Scholarship.
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Rosebud Battlefield State Park
This National Historic Landmark on the rolling prairie of eastern Montana preserves the site of the June 17, 1876 battle that was a harbinger to the Battle of Little Bighorn.
The Battle of the Rosebud, known to the Northern Cheyenne as "Where the Girl Saved Her Brother", took place during the Campaign of 1876. Brigadier General George Crook, along with his Crow and Shoshone scouts, had come north from Wyoming with approximately 1000 troops looking for the Sioux and Northern Cheyenne villages of Chief Sitting Bull. On the morning of June 17, 1876, near the headwaters of Rosebud Creek, Crook was unprepared for the organized attack of an equal or even greater number of warriors lead by Sioux Chief Crazy Horse and Cheyenne Chiefs Two Moon, Young Two Moon and Spotted Wolf. The presence of thousands of warriors and soldiers on the rolling hills of Southeastern Montana made the eight-hour engagement one of the largest battles of the Indian wars. This battle was also exceptionally significant because the Native Americans fought as an army with great intensity to defend their traditional land. Crook was stopped in his advance and the Native Americans were emboldened by the success.
Eight days later, because Crook's troops were withdrawn from the war zone to resupply, they were not available to support Colonel Custer and his troops at Little Bighorn. The Lakota and Northern Cheyenne warriors overtook Colonel George Custer and his 263 soldiers at Little Bighorn. This defeat shocked the nation celebrating its Centennial and ultimately led to a counter attack and to the Lakota's loss of the Black Hills.
Remote, quiet and undeveloped, the 3052 acre state park was made a National Historic Landmark in 2008 commemorating the Battle of the Rosebud, "Where the Girl Saved Her Brother." The site remains much the same as it was during the time of the battle in the 1870's, though the park does not cover the entire battle grounds.
Also within the park boundaries, the land has a full and long history going back before the battle as well as afterwards. For generations before, many peoples used the Rosebud Valley for hunting and gathering, including a cliff site used as a "buffalo jump" still marked with petroglyphs. These can be observed with a short hike in the gap to the cliffs.
In the years after the battle, this area was opened to homesteading and Rosebud Creek went through an era of ranching families grazing and plowing much of the land. In 1978, the State of Montana acquired the property to set aside for a state park.
Rosebud Battlefield is a wonderful place of solitude to reflect on America's history, take a picnic, enjoy a hike and look for wildlife. Bring your camera, hiking boots and plenty of time to appreciate the history and the sacredness of this place. Mowed paths for walking are maintained to the Kobold Buffalo Jump and up to the top of Crook's Hill, but feel free to explore across the grassy hills of the entire park. See the beautiful view from Crook's Hill, then wander over to Conical Hill to get the Native American perspective. Trek up to Van Vliets Ridge and imagine the cavalry charge down the slopes.
Please be aware there are restrictions on the use of metal detectors, digging, collecting or removal of artifacts, and bikes are allowed on existing roadway only. The leash law for pets is in effect from 4/1 until the opening day of upland game bird season. From April 1st to September 1st, no discharge of firearms is allowed. Visitors must watch for rattlesnakes in this area. The area is undeveloped and is adjacent to the Crow Indian Reservation. To reach the park, one must drive through private property—please respect the privacy and property of others. Contact the park office at Tongue River Reservoir State Park regarding hunting regulations and other questions.
The Park is 3052 acres at an elevation is 4,300 feet.
Camping and other recreational activities are available at nearby Tongue River Reservoir State Park 13 miles south. For other nearby historical opportunities and amenities, Sheridan, Wyoming is 40 miles south and Little Big Horn Battlefield National Monument is 48 miles to the northeast.
Some amenities are seasonal. Check with the park for availability.
Interpretive Display
Pack-in/Pack-out
Toilets (Vault)
Seasons & Hours
Hours listed below are normal operating hours and may not apply when there is a special restriction or closure. Check Alerts and Closures in the tab below.
Open all year/ Day Use Only
Alerts & Closures
Restrictions and Closures
See a current list of Restrictions and Closures on State Parks and other FWP lands that may be affected by floods, fire, drought or major maintenance activities.
Visit the State Parks Fees & Regulations page for park rules.
Contact the park manager for open volunteer positions at Rosebud Battlefield State Park.
For complete position descriptions, application forms, and details about Montana State Parks volunteer programs, visit the Volunteers page.
Montana Residents
Montana residents who pay the $9 state parks fee with their annual vehicle registration have no daily entrance fees to state parks. For residents who don't include this in their vehicle registration, non-resident day use fees apply.
Day use entrance fee with a vehicle: $8
Day use entrance fee as a walk-in, bicycle or bus passenger: $4
With a Nonresident Entrance Pass: Free
c/o Tongue River Reservoir
Decker, MT 59025
Email: raymond.schell@mt.gov
Tips to stay safe while getting outdoors
To keep our state parks safe and accessible to all, make sure you know before you go, practice physical distancing, plan ahead, play it safe, explore locally, leave no trace, respect wildlife, and do your part to build an inclusive outdoors.
Visit the Recreate Responsibly page for more information
HC 42, Box 642
Busby, MT 59016
Latitude/Longitude:
(45.22031 / -106.97590)
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View the park map to learn more about amenities, parking and camping details
Park fees
Learn about fees and regulations for all Montana State Parks
View Fees >
Meet The Park Manager
Raymond Schell
Raymond Schell, Park Manager at Tongue River and Rosebud Battlefield state parks, had over 20 years' experience working in parks in Arizona and Alaska before joining the Montana State Parks system in 2017. Prior to moving to Tongue River, Raymond managed White Tank Mountain Regional Park, a popular 30,000 acre recreation area west of Phoenix, AZ. Before that, he worked for 8 years at Denali National Park, AK.
Tongue River Reservoir


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Foreign minister attending Brussels NATO meeting this week ({{contentCtrl.commentsTotal}})
Foreign minister Sven Mikser (SDE). Source: Priit Mürk/ERR
Foreign minister Sven Mikser (SDE) is in Brussels this week, attending a meeting with counterparts from other NATO countries.
Topics on the table at the meeting, which takes place on Tuesday and Wednesday, include transatlantic relations and security, covering issues concerning the Intermediate-Range Nuclear Forces Treaty (INF), events on NATO's southern flank (especially the recent Kerch Strait incident), and the situation in the Western Balkans, spokespersons for the Ministry of Foreign Affairs said.
Ukrainian and Georgian partners are to join the meeting at its opening, discussing regional security. Estonia has already condemned Russia's naval attack against Ukrainian vessels in the Kerch Strait, which took place on 25 November.
Gross breach of international law
"This is another gross breach of international law by Russia as well as an attack against the sovereignty and territorial integrity of Ukraine," Mr Mikser said ahead of the NATO meeting.
Immediate release of the Ukrainian crew and their ships, together with free access via the Kerch Strait and in the Sea of Azov which the strait separates from the Black Sea, in accordance with international law, is paramount, according to Mr Mikser.
The security situation in Afghanistan is also due for discussion, including with partners of the NATO-led training, advice and assistance Resolute Support Mission (RSM), which Estonia is to continue contributing to, having already sent an infantry unit serving as part of a UK contingent in Kabul in May, as well as the several Estonian civic experts and mine clearance instructors present in Afghanistan. A bill concerning the extension of Estonia's participation is also currently going through parliament.
sven mikserministry of foreign affairsnatoestonia in natoestonian ministry of foreign affairskerch strait incident
Mikser: EU must respond to Russia's activities with concrete steps
NATO open door policy set to continue says foreign minister
Mikser in Brussels: NATO allies united in support of Ukraine and Georgia
Estonia stance on Kerch Strait amongst clearest, says Ukrainian portal
Estonia to continue development cooperation with Afghanistan
Riigikogu drafts statement in support of Ukraine
Mikser wants additional sanctions on Russia
Ministry summons Russian ambassador over Kerch Strait incident
Estonia condemns Russian attack on Ukrainian vessels in Kerch Strait


We are very pleased to announce our new planned group trips to India in 2018. The first one will lead you to the most famous attractions of Kerala and Tamil Nadu as well as off the beaten track places where you will have direct contact with the local population, like artisan villages, gypsy communities, special need schools to name a few. At the same time you have ample opportunities to explore old Hindu temples like the famous Meenakshi Amman temple of Madurai, seek darshan with Amma, one of the most prominent modern day saints in the Amiritamayi Ma ashram, explore the Jew town of Mattancherry in Fort Cochin, dip into the Arabian sea at Varkala beach, relax on a houseboat floating through the tranquil backwaters and spot wildlife in the Periyar Tiger Park in the Western Ghats. On this 20-day tour, you will palpate the fascinating millenarian culture of India traveling like an insider! A large part of the proceeds of this tour will directly benefit some of the most disadvantaged sectors of the population of Tamil Nadu.
Get USD 100 off our tour price if you book by 31st March 2017. Limited spaces. Tour bookings are open until max. 15th September 2017.


The development of an efficient prophylactic HIV vaccine has been one of the major challenges in infectious disease research during the last three decades. Here, we present a mini review on strategies employed for the development of HIV vaccines with an emphasis on a well-established vaccine technology, the killed whole-virus vaccine approach. Recently, we reported an evaluation of the safety and the immunogenicity of a genetically modified and killed whole-HIV-1 vaccine designated as SAV001 . HIV-1 Clade B NL4-3 was genetically modified by deleting the nef and vpu genes and substituting the coding sequence of the Env signal peptide with that of honeybee melittin to produce an avirulent and replication efficient HIV-1. This genetically modified virus (gmHIV-1 NL4-3 ) was propagated in a human T cell line followed by virus purification and inactivation by aldrithiol-2 and γ-irradiation. We found that SAV001 was well tolerated with no serious adverse events. HIV-1 NL4-3 -specific polymerase chain reaction showed no evidence of vaccine virus replication in participants receiving SAV001 and in human T cells infected in vitro. Furthermore, SAV001 with an adjuvant significantly increased the antibody response to HIV-1 structural proteins. Moreover, antibodies in the plasma from these vaccinations neutralized tier I and tier II of HIV-1 B, A, and D subtypes. These results indicated that the killed whole-HIV vaccine is safe and may trigger appropriate immune responses to prevent HIV infection. Utilization of this killed whole-HIV vaccine strategy may pave the way to develop an effective HIV vaccine.
Despite proactive HIV/AIDS education and availability of effective combination antiretroviral therapy (cART), the AIDS pandemic continues. WHO and UNAIDS estimate that close to 37 million people are currently living with HIV infection and approximately 40 million HIV related deaths have occurred since the discovery of HIV-1 . However, vaccine development against HIV continues to struggle due to unresolved questions on the exact components of the human immune response that confer protection against HIV infection. Similar to multiple effective anti-viral vaccines, a safe, effective, and globally accessible HIV vaccine must be developed to end the AIDS pandemic.
The first human clinical trial of Vaxgen's AIDSVAX was based on a recombinant HIV-1 gp120. This trial showed that 5.7% of 3330 vaccinated high-risk volunteers contracted HIV while 5.8% of the 1679 placebo control group contracted HIV . The monomeric form of recombinant gp120 induced antibodies but failed to neutralize the virus. We now know that the native conformational epitopes of the trimeric form of gp120 are key to generating neutralizing antibodies [4, 5].
The second HIV vaccine human clinical trial was conducted by Merck. This trial was known as the STEP study or Phambili Trial which utilized a recombinant adenovirus 5 (Ad 5) vector carrying HIV-1 gag, pol, and nef genes . Merck's recombinant Ad 5 vaccine was neither effective in preventing infection nor in reducing viral loads. Trial results showed that 3.2% of 741 vaccinated volunteers became infected, but only 2.7% of the 762 individuals in the placebo group became infected with HIV-1 during the STEP study . Merck discontinued STEP study in September 2007. The reason for the higher rate of infection among the vaccine group was believed to be a result of preexisting Ad 5 antibodies in these subjects.
In September 2009, the US National Institutes of Health announced the results of a Phase III human clinical trial, known as RV144, conducted in Thailand. RV144 consisted of a recombinant Canary poxvirus carrying HIV-1 gag, pol and env genes as a prime vaccine known as ALVAC developed by Sanofi Pasteur followed by a boost vaccine of gp120 based AIDSVAX. The results of this third human trial showed that 51 of 8197 (0.6%) vaccinated individuals became infected while 74 of 8198 (0.9%) placebo control group contracted the HIV . Thus, the vaccine cut the risk of infection by 31.2% after 42 months [8–10]. While the RV144 vaccine did not induce any broad spectrum neutralizing antibodies, it did induce non-neutralizing antibodies presenting potent antibody-dependent cellular cytotoxicity (ADCC) activity which could have decreased transmission [11, 12]. Even though these results showed potential for an HIV vaccine, it was not enough to overcome the HIV epidemic and develop a commercialized product. There will be a further large scale HIV vaccine trial conducted in South Africa with this vaccine. Indeed, the HVTN 702 trial will be an extension of the RV144 clinical trial using the canarypox-based vaccine with bivalent gp120 protein. This study will enroll 5400 HIV-uninfected men and women volunteers (NIAID, NIH). It will be interesting to see whether or not the HVTN702 trial will meet expectations.
Finally, the fourth vaccine trial used a combination of a DNA-based vaccine followed by a recombinant Ad 5-based vaccine boost (known as the HVTN 505 trial conducted by the US NIAID) which showed that 41 of 1250 individuals (3.3%) in the vaccine group and 30 of 1244 individuals (2.4%) in the placebo group contracted HIV during the trial . Like Merck's STEP trial, this DNA priming followed by a recombinant Ad 5 boosting combination vaccine also had a higher rate of infection among the vaccinated group compared to the non-vaccinated control group.
There are three types of commercially available vaccines. These include attenuated live virus vaccines, recombinant protein based subunit vaccines, and killed whole-virus vaccines. Although there have been many successful live attenuated viral vaccines developed against many human and animal viral diseases, the attenuated live virus vaccine strategy is not an option for HIV, because the proviral DNA of live attenuated HIV will be integrated into the host chromosomal DNA to establish a persistent infection.
With respect to the subunit vaccine strategy, two successful viral subunit vaccines have been developed. The 22 nm virus-like particles (VLP) of human hepatitis B virus S antigen and the L1 protein-based VLP of the human papilomavirus vaccine are effective subunit vaccines with proven efficacy. VaxGen has already attempted recombinant gp120-based vaccine for HIV. However, the AIDSVAX of VaxGen failed to provide protective immunity due to its monomeric form of gp120 which cannot generate neutralizing antibodies. Thus, scientists have tried to express the trimeric form of gp120 in order to induce broadly neutralizing antibodies [14, 15].
The third commercially available antiviral vaccine is the killed-whole virus vaccine. This vaccine strategy has prevented many viral diseases such as polio, influenza, rabies, and hepatitis A [16–19]. In addition, there are at least 16 licensed killed whole-virus vaccines available to protect against various viral diseases in animals. However, the killed whole-virus vaccine approach has been neglected for HIV vaccines due to risks associated with incomplete inactivation of HIV and other technical challenges. Interestingly, only one group (Remune) previously attempted a killed whole-HIV vaccine strategy. However, the lack of gp120 on the virion surface of the Remune vaccine and other safety concerns led to the discontinuation of Remune's project .
The killed whole-HIV-1 vaccine approach has great merit as it has the potential to present multiple viral antigens to the immune system in their native conformation. Thus, two groups have suggested that it is time for another look at inactivated (killed) HIV vaccine for prevention of HIV infection [21, 22]. The challenges in developing a killed whole-HIV vaccine include the lack of techniques for high level virus production and the safety issues associated with virus production in large quantities. We overcame these problems by deleting the nef gene which contributes to AIDS pathogenesis [23–26] and substituting the envelope glycoprotein signal peptide gene to achieve high level virus replication [27, 28].
We have shown that the natural HIV-1 Env protein signal peptide is highly inefficient for intracellular processing of HIV glycoproteins , however replacement of the HIV-1 Env protein signal peptide with the signal peptide of honeybee melittin results in enhanced protein expression, glycosylation, folding, intracellular transport, and processing [27, 28]. Furthermore, due to the overlapping reading frame of the vpu gene with that of the HIV-1 natural signal peptide gene, our killed whole-HIV-1 (SAV001) has a deletion of the vpu gene as well. The genetically modified HIV-1 NL4-3 (gmHIV-1 NL4-3 ) was propagated in the A3.01 human T cell line, purified through sucrose gradient and inactivated by aldrithiol-2 [29, 30] and 30 kGy of gamma irradiation . This purified SAV001 showed intact virion morphology under electron microscopy and its capability to aggregate AA2 and A3.01 human T lymphocytes in culture indicate that functional gp120 peplomers are present on the purified virions .
The major concern for the killed whole-HIV vaccine is the incomplete inactivation of HIV. One viable HIV virion during the virus inactivation process is indeed one too many for a killed whole-virus vaccine. To ensure the safety of the vaccine, we deleted the nef gene for attenuation, and used both chemical (aldrithiol-2) and physical (γ-irradiation) inactivation to provide complete killing. The test for in vitro replication of SAV001 using sensitive methods showed that virus replication was absent even after ten consecutive passages in human T lymphocytes .
Furthermore, following immunization, highly sensitive, external-nested polymerase chain reaction (PCR) amplifications using vaccine virus-specific primers on the viral RNA in plasma confirmed the lack of any virus genome originating from the vaccine. In contrast, the external-nested PCR amplification by generic HIV-1 primer sets amplified HIV-1 RNA despite undetectable viral loads by the less sensitive Roche Amplicor assay (<50 copies/ml). Pyrosequencing of these PCR products before and after immunization with SAV001 again confirmed the lack of HIV-1 NL4-3 in over 71,000 HIV sequence reads and the presence of only patient-specific HIV-1 strains. These results provide very strong evidence that aldrithiol-2 treatment followed by gamma irradiation (30 kGy) completely inactivate HIV-1 and ensure the safety of the vaccine recipients .
Despite the complete inactivation of gmHIV-1 NL4-3 virus used in our study, we only enrolled HIV-1 positive asymptomatic volunteers based on discussions with the US FDA. Therefore, even though the study was designed to evaluate safety and tolerability, the nature of the study limited our ability to assess the immunogenicity of the vaccine formulations. Previous studies have shown that HIV-1 infection elicits antibody responses to proteins encoded by HIV-1 gag, pol and env genes, and the antibody response to various proteins appears at different stages of infections . As expected, most of the subjects in our study had a high baseline antibody titer against viral structural proteins. However, with a single SAV001 intramuscular vaccination, the humoral immune response was significantly increased by boosting the secondary anti-HIV antibody responses in vaccinated groups suggesting a strong immunogenicity of the SAV001 vaccine.
The importance of the design of immunogens, especially the trimeric form of the envelope glycoproteins, capable of inducing broadly neutralizing antibodies is the major focus of current HIV-1 vaccine research [33, 34]. Even though our killed whole-gmHIV-1 NL4-3 virus was purified and completely inactivated through combined chemical and physical inactivation processes, its Env glycoproteins were not readily shed and were still functional. Our results suggested that SAV001 vaccine can mimic natural infection through its native viral structure, especially the native form of envelope glycoprotein which is crucial for eliciting broadly neutralizing antibodies. Indeed, when compared to placebo control, our data supported this hypothesis as SAV001 was able to stimulate anti-gp120 antibodies in plasma that could recognize trimeric Env glycoproteins at the surface of infected cell and mediate ADCC. Although most of subjects in our trial were infected with HIV-1 subtype B, sera from these subjects neutralized not only subtype B but also subtypes D and A which is consistent with the notion that HIV-1 superinfection is not a common event [35–37]. Thus, an HIV-1 vaccine based on one subtype may be able protect against infections of other subtypes.
Vaccination with SAV001, the genetically modified and killed whole-HIV-1 vaccine, could enhance humoral immune responses including broadly neutralizing antibody production in HIV-negative individuals. Therefore, SAV001 represents a promising starting point for the development of a safe and effective prophylactic HIV-1 vaccine using the killed whole virus approach. This approach could be easily adaptable to include different subtypes of HIV-1.
CYK designed, conducted research on killed whole-HIV vaccine, and wrote the article. YG conducted research on immune responses. Both authors read and approved the final manuscript.
We thank the NIH AIDS Reagent Program for their supply of reagents. We thank Dr. Jimmy Dikeakos for editing this manuscript.
The authors' research is supported by the National Research Council of Canada Industrial Research Assistance Program and by Sumagen Canada Inc., as well as CIHR (HBF143165) and NIAID NIH (R01-AI084816).
AIDS vaccine blueprint. In: A challenge to the field, a roadmap for progress. New York: International AIDS Vaccine Initiative-IAVI; 2008 https://www.iavi.org.
Orchestra Therapeutics discontinues its HIV vaccine development program. 2007. http://www.biospace.com/News/orchestra-therapeutics-discontinues-its-hiv/63327.


Two of the four sites – Inner Temple and Middle Temple – were located centrally in the capital below the Strand to the east of Temple Bar, while Lincoln's Inn was just north of the Strand. Gray's Inn was positioned further north on a north-south axis along the western edge of Gray's Inn Road.
The students at the four Inns of Court also often organised and acted in plays and masques at winter festival times at the Inn's hall, priding themselves on their 'rhetorical' powers of persuasion, an essential part of a good lawyer's professional equipment. The tradition is still carried on today when Inn members create and perform in Christmas shows. However, in the Shakepearean period all four sites should be considered authentic ShaLT performance locations in their own right, since their indoor halls were used as performance spaces for visiting playing companies. Most famously, in 1594 the Lord Chamberlain's Men put on Shakespeare's The Comedy of Errors at Gray's Inn, whilst his Twelfth Night was staged at Middle Temple in 1602.


Digital Human Library: What is the Digital Human Library?
What is the Digital Human Library?
The Digital Human Library (dHL) is an online database of people who have agreed to share their expertise, knowledge, skills and experiences on a wide range of topics in a variety of subject areas. Using only a computer, a webcam, and Skype (a free software application), people in our community and communities around the world are connecting with teachers and students in classrooms to share their ideas, teach lessons, answer questions, and collaborate with educators to build a classroom program that better meets the needs of our children. Think of the Digital Human Library as you would a library, but instead of books, the resources that can be signed-out are the participants (people). These participants have agreed to be contacted by classroom teachers to collaborate on an activity, lesson, or presentation that would then be shared at an agreed upon time using Skype. So, in addition to the use of books and other classroom resources, students will now be able to engage with real people in real time using video calling software to learn curriculum based concepts and skills in a new and exciting way. The Digital Human Library brings the field trip experience into the classroom!


Looking for direction on adding 2 new DS4243 shelves into our existing FAS2040 environment. Focusing on properly distributing the addition of the 48 new disks.
If I understand things I've read, the larger the RAID size to better the performance is because the data is striped across more disks but if a disk fails then rebuilding will take much longer.
Is there any kind of charts for layout of disks based on the disk size?
16TB limit is not raw, it is usable, so e.g. the maximum spindle count for 1TB drives is 23 for ONTAP 7.3.4.
Bigger RAID groups are not necessarily better for performance (an aggregate can span multiple RGs, so can a volume), but there is less capacity overhead (fewer parity drives).
Thank you for all the documentation to read, TR and community posts. I will soak them in during the next couple of days. A quick question though, can the RAID group size be changed on an aggregate without data lost? If so, what is the proper way of doing so and what are the negatives of making such change. Thanks.
While you cannot subtract from a raidgroup you can modify the maximum size and add. It will warn you if certain raidsizes are not supported in earlier ontap releases.
I'd imagine its much easier using your gui of choice.
In general I prefer to decide on what my ideal raidsize is when I initially create the aggregate. Diverging from this setting shouldn't have any affect if one rg is 1-2 disks larger than the other and you are topping out its maximum size.
I usually think about it as trade off between rebuild times and performance or max usable capacity.
Changing raid group size won't affect existing raid groups unless you also add disks to aggregate.